JPH0840B2 - Active oxygen suppressing composition, method for producing the same, and blood pressure suppressing agent - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a composition for suppressing active oxygen, a method for producing the same, and a blood pressure suppressing agent. The present invention
Various diseases caused by active oxygen (O ₂ ⁻ ), for example,
Diseases caused by blood flow disorders (myocardial infarction, stroke, high blood pressure, menstrual cramps, stiff shoulders, neuralgia, back pain, hangover, etc.), adult diseases / internal diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty / skin diseases (stains, freckles) , Rough skin, chills, constipation, wrinkles, atopic dermatitis, etc.) are widely used for treatment and improvement.

[0002]

2. Description of the Related Art Superoxide dismutase (SOD
Say. ) Is a superoxide produced by one-electron reduction of oxygen molecule
-Oxide radical
cal, O₂ ⁻) Disproportionation reaction (equation below)
It catalyzes at a high speed and intracellular O₂ ⁻Enzyme that reduces concentration
Is. O₂ ⁻+ O₂ ⁻+ 2H⁺→ H₂O₂+ O₂  In recent years, O₂ ⁻If is harmful to the living body, it is its removal enzyme
This SOD exists to protect the living body from active oxygen toxicity.
This active oxygen is the cause.
From the perspective that it is useful for diseases that are thought to occur
Have studied the reaction mechanism, physiological mechanism, etc.
Oxygen-Molecular mechanism of generation, elimination and action in living organisms "
Second edition, published by Kyoritsu Shuppan Co., Ltd., edited by Minoru Nakano et al.) 2
23-230)]. In addition, active oxygen suppression activity in cancer cells
The fact that the SOD is low, and moreover, the direct relationship between SOD and carcinogenesis
The causal relationship is not clear, but SOD or SOD-like substances
It is reported that the injection of cancer cells into cancer cells suppresses their growth.
(P.64).

[0003]

If there is a food (composition) that is safe and has an active oxygen suppressing action, it will be extremely useful for human health and beauty, and there is a great need for it. In particular, in a situation where stress is high and many diseases are occurring as in recent years, the necessity thereof is great.

The present invention is a safe and excellent method in which a water-soluble green tea extract is contained in a fermentation aqueous solution obtained by fermenting rice bran and soybeans as raw materials at a pH of 7.5 to 10. Another object of the present invention is to provide a composition having an active oxygen suppressing action, a method for producing the same, and a blood pressure suppressing agent.

[0005]

From the above viewpoints, the inventors of the present invention diligently investigated whether a composition containing this active oxygen-inhibiting effect and a blood pressure-suppressing agent utilize a safe fermented product. As a result of various studies, the present invention has been completed by discovering that the purpose can be achieved by adding a water-soluble green tea extract to the above fermented aqueous solution. The active oxygen inhibiting composition of the first invention contains rice bran, soybeans and water, and has a pH of 7.5 with an alkaline agent.
Inoculation of Bacillus subtilis or Bacillus subtilis into a liquid medium adjusted to -10, culturing in an aerobic atmosphere, and a fermentation aqueous solution produced by filtration or an evaporation residue of the fermentation aqueous solution, at least water and ethanol. It is characterized by containing a water-soluble green tea extract extract obtained by extraction with a solvent containing one kind or an evaporation residue of the water-soluble green tea extract extract.
The third invention is a method for producing an active oxygen-suppressing composition in the form of an aqueous solution, and the seventh invention uses the active oxygen-suppressing composition of the first invention as a blood pressure suppressor.

The "green tea extract" is extracted from tea leaves with a solvent containing at least one of water and ethanol to make it water-soluble, and the concentration of the extracted components is 5 to 50. It can be about% by weight. Then, the mixing ratio of the green tea extract is, as shown in the second, sixth and eighth inventions, 1 to 50 parts by weight in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation aqueous solution. It can be a department. This is because if it is less than 1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 50 parts by weight, it becomes difficult to taste and drink.

Further, a composition in which flavoid is contained in the fermentation aqueous solution or the evaporation residue can be prepared. This "flavonoid" is widely distributed in the plant kingdom, and two phenyl groups have a structure similar to or similar to the pyran ring.
So-called C ₆ -bonded by sandwiching carbon atoms
It is a compound group consisting of C ₃ -C ₆ carbon skeleton. This flavonoid is 1 in the evaporation residue in the green tea extract.
It is contained in an amount of 3 to 14% by weight. And, the blending ratio of this flavonoid is 0.1 to 7 relative to 100 parts by weight of the fermented aqueous solution (for example, when the extract concentration is 40 to 50% by weight).
It can be about parts by weight. This is because if the amount is less than 0.1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 7 parts by weight, it becomes difficult to taste and drink.

The above-mentioned "rice bran" means rice germ, defatted rice germ, rice bran, defatted rice bran and the like, and "soybeans" means defatted soybean, quina flour, soybean flour, soybean dregs and hydrolysates thereof. Etc. Usually, these are added in an amount of 10 to 20 parts by weight of soybeans when the rice bran is 100 parts by weight. Further, a nutrient source such as a carbon source such as glucose usually used for fermentation can also be used. This fermentation condition is usually p
H is 7.5 to 10 (especially around 9), culture temperature is 40 to 4
It is about 5 ° C. In addition, protease can be used as a medium material. This case is useful because it further decomposes the soybean peptide.

The above-mentioned "fermentation aqueous solution" may be a solution obtained by culturing a cultured and fermented aqueous solution as it is, a solution obtained by post-treatment such as decolorization, or a concentrated solution obtained by concentrating the solution. As described above, the evaporation residue (solid content) of this fermentation aqueous solution may be used.

Further, a water-soluble green tea extract or a composition in which at least one of phytic acid and its salt is contained in the evaporation residue can be prepared. The above-mentioned "phytic acid" may be synthetic phytic acid or phytic acid extracted and isolated. Further, as "the salt", a non-toxic salt is used, and a salt with a metal, an organic base, a basic amino acid, an organic ester residue or the like is used, and examples thereof include potassium, sodium, arginine, ornithine, lysine, histidine. , Glucamine, monoethanolamine and the like. Since this phytic acid exhibits acidity, a salt thereof having a pH of about 6 to 8 is usually used. Usually, this salt is water-soluble. Further, this phytic acid and its salts are contained in large amounts in the above-mentioned fermented aqueous solution, and when converted from the phosphorus concentration, 100 parts by weight of this evaporation residue,
It contains 6 to 7 parts by weight of phytic acid equivalent. Therefore, it is considered that even if a predetermined amount of green tea extract is contained in phytic acid or the like, the same effect can be surely obtained as in the case of the above invention.

[0011]

The present invention will be described in detail with reference to the following examples. (1) Production of Fermentation Aqueous Solution and Preparation of Active Oxygen Suppressing Composition and Blood Pressure Suppressing Agent Test Product First, a fermentation aqueous solution is produced by the following method. That is, defatted rice bran; 30 kg, soybean meal (or defatted soybean);
3 kg, bittern, 5 kg, water; 500 kg were used.
The pH is around 9. The above medium was sterilized at 121 ° C. for 30 minutes, then cooled, and then Bacillus natto (manufacturer;
Naruse Fermentation Research Institute) inoculated with 0.05 kg, 40-4
The cells were cultivated by aeration and stirring at 5 ° C. for about 48 hours.

Then, this culture was squeezed and filtered, treated with activated carbon and perlite to deodorize and decolorize, and an almost transparent fermentation aqueous solution (fermented extract) was obtained. As the activated carbon, various types such as powdered activated carbon (activated carbon S, activated carbon K, etc.) and granular activated carbon (activated carbon SG, etc.) can be used, and as the perlite, “Perlite No. 4180” (Daikaline Orient Co., Ltd.) Manufactured) was used. This fermented aqueous solution is a colorless or pale yellow transparent liquid, has a flavor of fermentation, water content of 92 to 94% (according to a normal pressure heating drying method), and phosphorus of about 300 to 400 mg / 100 g (according to a vanad molybdenum analysis method). .), General live bacteria, E. coli,
Mold, yeast and arsenic, lead, cadmium and tin were not detected.

As the test product 1 of the present active oxygen-suppressing composition and blood pressure suppressing agent, 3 parts by weight of green tea extract (water or ethanol extract, solid content concentration; 48% by weight, specific gravity; 1.07 to
1.09) was blended (evaporation residue ratio: fermentation aqueous solution content / extracted extract content = 100: 20). The solid content concentration was determined as a Brix value, and the concentration of ethanol and glycerin (mainly ethanol) was about 50% by weight, and the concentration of water was about 2.6% by weight.

(2) Performance Evaluation (A) Regarding Active Oxygen Suppression Action A test on H ₂ O ₂ under the following conditions (Table 1,
Table 2), a test for OH (Table 3), and a test in an O ₂ -xanthine system (Table 4) were performed, and the results are shown in each table. As a test product, a 5-fold diluted solution with water was used as the test product 1.

[0015]

[Table 1]

[0016]

[Table 2]

[0017]

[Table 3]

[0018]

[Table 4]

As shown in Tables 1 to 4 above, this test product 1
Shows that the active oxygen suppressing action is superior to that of the control product. From the above, it can be said that the active oxygen inhibition activity is 460 units / ml (1 cup 10 ml). Furthermore, the following tests were conducted for the synergistic effect of the fermented aqueous solution and the green tea extract. That is, each sample (No. A to C) having the following blending ratio was prepared. Each of the above samples was measured for active oxygen inhibition activity by the measurement method shown below.

[Measurement Method of Active Oxygen Suppression Activity] This method is a method using an EPR (electron spin resonance) apparatus, and has recently been carried out as a test item by the Japan Food Analysis Center; It is a method that came to be known. That is, first, each homogenized sample 1
0.1 M phosphate buffer (pH 7.8) is added to g to extract sufficiently to obtain an extract. Then, the following reagents are mixed. 2 mM hypoxanthine / phosphate buffer 50 μl 5.5 mM DETAPAC (diethnetr
iaminepentaacetic acid) / phosphate buffer 35 μl extract or standard SOD (superoxide dismutase) 50 μl DMPO (5,5-dimethyl-1-pyrro)
line-1-oxide) 15 μl 0.4 units / ml xanthine oxidase / phosphate buffer 50 μl Then, this mixed solution is sucked into a special flat cell (manufactured by JEOL Ltd., capacity: about 130 μL), and ESR device (JEOL) (Manufactured by JES-FR80, manufactured by Co., Ltd.), the sweep was started 40 seconds later, and the measurement was performed under the ESR operating conditions shown below. In addition, this active oxygen suppression activity measurement is a measurement of the solution one day after the sample preparation. [ESR operation conditions] temperature room temperature microwave output 8 mW magnetic field 334.7 mT ± 5 mT modulation 100 kHz, 0.79 × 0.1 mT amplification factor 3.2 × 100 response time 0.1 seconds sweep time 2 minutes

Then, the ratio of the peak height of Mn ^{2+ to} the peak height of the measurement signal is determined. Then the standard SOD (0-
15 units / ml), a calibration curve was prepared, and the values obtained from the extract were interpolated to calculate the erasing activity in the sample. The results are as follows. The unit is UNIT / g. “Activity additive theoretical value” is the sample No. It is simply integrated by using the respective active oxygen inhibition activity values of A and B. No. containing the fermented aqueous solution and the green tea extract In C, the actual active oxygen suppression activity value is 1.15 times the theoretical value of active addition, indicating that there is a synergistic effect.

(B) Fluctuations in blood pressure during elevated blood pressure in rats Under the following conditions, 100 parts by weight of the fermentation aqueous solution was mixed with 1.5 parts by weight of the green tea extract to give a test product 2. The change in blood pressure due to the administration of this test product 2 was evaluated. The result is shown in FIG.

[Test conditions] Animal: rat; 3 males for control, 4 males for test product,
7 in total. The age of this rat is 6 weeks, and its blood pressure is 150 mmHg or higher. It should be noted that this rat has a genetic property that blood pressure increases from 3 weeks of age (hereinafter referred to as SH
Called R. ). Test administration method: Free intake by water supply pin. Indoor: Temperature 23 ± 1 ° C, humidity 50% ± 10%. Beverage: Glucose and citric acid were added to a 10% solution of the test product 2 (corresponding to SOD) to make it easy to drink. Blood pressure measurement; once every 2 weeks. Feed used: Common feed (“MF” manufactured by Oriental Yeast Co., Ltd.) was used in all cases. The results show that the use of this test product 2 has an effect of suppressing an increase in blood pressure as compared with the control group.

(C) Fluctuation of blood pressure in the hypertension completion stage of rats The fluctuation of blood pressure due to administration of Test Product 2 was evaluated under the same conditions as above except the following conditions. The result is shown in FIG. [Test conditions] Animal: rat (SHR above); 4 males for control, 4 males for test product, 8 in total. The age of this rat is 12 to 15 weeks, and its blood pressure is 180 mm.
It is Hg or more. The other conditions are the same as above. According to this result, as shown in FIG. 2, it has an effect of suppressing hypertension and, at the same time, an excellent suppressing effect with the passage of administration time.

(D) Monitor test 60 parts by weight of the above fermented aqueous solution, 1.2 of the above green tea extract
The following good results were obtained as a result of a monitor test in which a test product (10 ml portion) containing 10 parts by weight of oligosaccharide and mainly other parts of purified water was used for humans. A (58 years old, female) [Symptoms before the start of the monitor] His face was dark, the climbing of stairs was very difficult, his blood pressure was 165, and he was constantly taking a depressant. [Results after 2 drinks daily] My legs became lighter in about 1 week. The antihypertensive drug was stopped and the blood pressure became 130 in about 3 weeks. After two months, my face turns white so much that I can't see it. The 10-year-old started to look younger.

B (70 years old, male) [Symptoms before the start of the monitor] Even in everyday life, my cardiorespiratory function was weak, so when I climbed the stairs, I felt out of breath and dragged my legs. [Results after 3 daily drinks (morning, noon, evening)] In about 2 weeks, the body begins to feel light. After about a month, I can't breathe out even when I climb the stairs. Four months later, when I was asked to see cardiopulmonary function at the hospital, it was much better. For the first time this winter, I was fine without having to be hospitalized without catching a cold.

C (52 years old, female) [Symptoms before starting monitoring] He was suffering from diabetes and constipation due to high blood pressure. The blood pressure is 180 at the top and 95 at the bottom. [Results after 2 daily drinks (for 2 months) then 1 daily drink]
From the 4th day, urine is about twice as much as before and my body is feeling better. The stools will also appear once every three days. Then every two days. Two months later, my doctor tells me that I don't have to take blood pressure lowering drugs. His blood pressure was 145 on the upper side and 78 on the lower side, and he no longer needed medicine.

D (23 years old, female) [Symptoms before the start of monitoring] Constipation was severe 1 from an early age.
She had a flight once every two weeks from the 0th day. There were always small pimples on the face, and the skin was dry.
It was rather slender, but the lower abdomen was protruding like an infant figure, and I was considerably worried. [Results after 5 drinks for the first 2 days and 2 drinks every day] The following morning, there was a bowel movement. There are daily flights than that. After a month and a half, my face was completely smooth and my skin was shiny. The makeup glue has improved very much.

E (70 years old, female) [Symptoms before the start of monitoring] He was hospitalized for high blood pressure of 200. [Results after 2 daily drinks (morning and evening)] Blood pressure dropped in about 10 days and I was able to be discharged from the hospital. Currently 140.

F (68 years old, female) [Symptoms before the start of monitoring] My body was tired and tired and my body was stiff. [Results after 2 daily drinks (morning and evening)] Moisturized skin and improved makeup paste from the 2nd day. I was less tired than in the second week and I started to move as I wanted.

G (male, 45 years old) [Symptoms before starting monitoring] Diabetes was frequently hospitalized for 2 to 3 weeks, and the liver was hurt during the treatment. [Results after 2 daily drinks] After 2 months, my doctor told me that my liver had completely improved, and after 4 months I had better diabetes, and I was able to get back to work by returning to work. There is no need for hospitalization.

(E) Summary of Effects of Examples As described above, the main fermentation aqueous solution is safe because harmful bacteria and heavy metals are not recognized and the fermented aqueous solution is a natural fermented product of completely natural raw materials. Further, as shown in the above results, since the active oxygen suppressing effect is excellent, the results show that the blood pressure suppressing effect is excellent as described above. Since it is excellent in this active oxygen suppressing effect, it can be said that, in addition to the blood pressure suppressing effect, the effects resulting from this active oxygen suppressing effect are expected in the same manner. Furthermore, the present inventors believe that this fermented aqueous solution has a function of effectively incorporating the green tea extract into the body. Regarding this point, it is necessary to wait for further detailed research, but in any case, if the main fermentation aqueous solution and the green tea extract are used in combination, the active oxygen suppression effect is excellent, and it can be safely consumed by humans and Very useful for Further, the fermentation aqueous solution (or its evaporation residue) and the water-soluble green tea extract (or its evaporation residue) are
Since all of them are water-soluble, they can be dissolved in water to be used as an aqueous beverage, food, etc. as shown in the above-mentioned test, and therefore can be said to be excellently absorbed in the body. Further, as shown in the monitor test of (D) above, a portion (refers to a small container shape containing fresh milk).
As being tested as a beverage (food) in which the test product (aqueous solution) is placed in a mold container, various aqueous solution products (beverages, drinks, etc.) can be easily prepared.

The present invention is not limited to the specific examples described above, and various modifications may be made within the scope of the present invention depending on the purpose and application. That is,
The composition consisting of the above fermented aqueous solution and the water-soluble green tea extract, etc. is a powder product impregnated with a liquid-absorbent powder, a granulated product, a tablet prepared by blending with other powder components (filler etc.),
Alternatively, it can be used as a microcapsule, a soft capsule, a hard capsule, a candy, a jelly, a biscuit or the like filled or blended. Further, the product form obtained by filling these compositions in a predetermined container is not particularly limited, and may be, for example, a potion type as mentioned in the above examples, or a container having another shape. It may be a powdery product filled in a stick-shaped container (bag), or may be a conventional soft drink, a drink,
You may mix and disperse in a dairy product, an oil-ized product, etc., and may use it. The dispersion may be water-in-oil type or oil-in-water type.

[0034]

The composition of the present invention has an excellent effect of suppressing active oxygen. Therefore, by using this active oxygen suppressing composition,
Various diseases are active oxygen causes, for example, diseases caused by blood flow disorders (myocardial infarction, stroke, high blood pressure, menstrual cramps, shoulder stiffness, neuralgia, low back pain, hangover, etc.), adult diseases, medical diseases (cancer, nephritis, hepatitis , Diabetes, etc., beauty / skin diseases (stains,
It is considered to exert an excellent effect on the treatment and improvement of freckles, rough skin, coldness, constipation, wrinkles, atopic dermatitis, etc.). Further, according to the production method of the present invention, a water-soluble composition having an excellent effect of suppressing active oxygen can be easily produced and prepared. The blood pressure inhibitor of the present invention has an excellent blood pressure inhibitory effect. Further, since the present invention is a water-soluble active oxygen-suppressing composition and a blood pressure-suppressing agent, it has excellent absorption effects and can be applied to a wide range of fields such as water-soluble beverages and foods.

In particular, since the fermented aqueous solution used as the main component in the present invention is a natural fermented product of completely natural raw materials, harmful bacteria and heavy metals are not observed, and it is safe and originally it has a function of suppressing active oxygen. Since rice bran and soybean products useful for blood pressure control are used as raw materials and they also contain useful nitrogen compounds (amino acids, etc.), ash, phosphorus compounds, etc., a very well-balanced active oxygen suppressing composition and blood pressure suppressing agent. Is. Furthermore, since the green tea extract also uses a natural product, the active oxygen suppressing composition and the blood pressure suppressing agent containing it are excellent in safety.

[Brief description of drawings]

FIG. 1 is a graph showing changes in blood pressure during SHR blood pressure elevation in an example.

FIG. 2 is a graph showing fluctuations in blood pressure in the SHR hypertension completion period in Examples.

Claims (8)

[Claims] 1. A liquid medium containing rice bran, soybeans and water, and having a pH adjusted to 7.5 to 10 with an alkaline agent, inoculated with Bacillus subtilis natto or Bacillus subtilis and cultured in an aerobic atmosphere, At least one of water and ethanol is added to the fermentation aqueous solution produced by filtration or the evaporation residue of the fermentation aqueous solution.
A composition for suppressing active oxygen, comprising a water-soluble green tea extract extract extracted with a solvent containing seeds, or an evaporation residue of the water-soluble green tea extract extract. 2. The green tea extract is 1 in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquor.
The active oxygen suppressing composition according to claim 1, which is mixed in an amount of -50 parts by weight. 3. A liquid medium containing rice bran, soybeans and water, and having a pH adjusted to 7.5 to 10 with an alkaline agent, inoculated with Bacillus natto or Bacillus subtilis and cultured in an aerobic atmosphere, A fermented aqueous solution is produced by filtering, and the fermented aqueous solution is mixed with a water-soluble green tea extract extract produced by extraction with a solvent containing at least one of water and ethanol to suppress the active oxygen in the form of an aqueous solution. A method for producing an active oxygen-suppressing composition, which comprises producing the composition. 4. The blending ratio of the soybeans is the rice bran 1
The method for producing an active oxygen suppressing composition according to claim 3, wherein the amount is 10 to 20 parts by weight when the amount is 00 parts by weight. 5. The method for producing an active oxygen suppressing composition according to claim 3, wherein the pH during culture is 8 to 10. 6. The green tea extract is 1 in terms of evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation broth.
The method for producing the active oxygen suppressing composition according to any one of claims 3 to 5, which is mixed in an amount of -50 parts by weight. 7. A liquid medium containing rice bran, soybeans and water and adjusted to pH 7.5 to 10 with an alkaline agent is inoculated with Bacillus subtilis natto or Bacillus subtilis and cultured in an aerobic atmosphere. At least one of water and ethanol is added to the fermentation aqueous solution produced by filtration or the evaporation residue of the fermentation aqueous solution.
A blood pressure suppressor comprising a water-soluble green tea extract extract extracted with a solvent containing seeds, or an evaporation residue of the water-soluble green tea extract extract. 8. The green tea extract is 1 in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation broth.
The blood pressure suppressing agent according to claim 7, which is mixed in an amount of -50 parts by weight.