JP5294372B2 - How to store microorganisms - Google Patents
How to store microorganisms Download PDFInfo
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- JP5294372B2 JP5294372B2 JP2007098933A JP2007098933A JP5294372B2 JP 5294372 B2 JP5294372 B2 JP 5294372B2 JP 2007098933 A JP2007098933 A JP 2007098933A JP 2007098933 A JP2007098933 A JP 2007098933A JP 5294372 B2 JP5294372 B2 JP 5294372B2
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- powdery mildew
- braun
- takamatsu
- var
- nomura
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Landscapes
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Abstract
Description
本発明は、微生物の保存の分野に関する。より具体的には、本発明は、人工培養が不可能である微生物の保存方法に関する。 The present invention relates to the field of preservation of microorganisms. More specifically, the present invention relates to a method for preserving microorganisms that cannot be artificially cultured.
従来より、細菌等の微生物を保存する技術として、（１）植え継ぎ法、（２）凍結保存法および（３）凍結乾燥保存法等が知られている（例えば、非特許文献１および２）。（１）の植え継ぎ法は、生育の早い栄養培地で寒天斜面培地あるいは斜面のない固体培地を作り、画線あるいは穿刺で植え継いでゆく方法である。（２）の凍結保存法は、微生物を１０〜２０％のグリセロール含有凍結保存液またはスキムミルク含有凍結保存液として懸濁し、これを−８０℃に凍結するものである。（３）の凍結乾燥保存法は、分散液に懸濁した微生物をアンプルに収容し、これを凍結乾燥機により真空処理して凍結状態のまま水分を直接昇華させる方法である。 Conventionally, as a technique for preserving microorganisms such as bacteria, (1) planting method, (2) cryopreservation method, and (3) freeze-drying preservation method are known (for example, Non-Patent Documents 1 and 2). . The planting method of (1) is a method in which an agar slant medium or a solid medium without a slant is made with a fast-growing nutrient medium and planted by streaking or puncture. In the cryopreservation method (2), microorganisms are suspended as a 10-20% glycerol-containing cryopreservation solution or skim milk-containing cryopreservation solution and frozen at −80 ° C. The freeze-drying preservation method (3) is a method in which microorganisms suspended in a dispersion are accommodated in an ampoule and vacuum-treated with a freeze-dryer to directly sublimate moisture in a frozen state.
いずれの方法においても、微生物の長期保存方法は、人工培養できる微生物を対象として開発されてきた。なぜなら、上記（１）植え継ぎ法が微生物の人工培養を必要とするのは、当然のことではあるが、（２）凍結保存法および（３）凍結乾燥保存法においても、保存する材料として培養した微生物を用いることが必須だからである。従って、人工培養不可能な微生物の長期保存方法については、開発されていなかった。 In any of the methods, a method for long-term preservation of microorganisms has been developed for microorganisms that can be artificially cultured. This is because the (1) transplanting method requires artificial culture of microorganisms, but it is natural that (2) the cryopreservation method and (3) the freeze-drying preservation method are also cultured as materials to be preserved. This is because it is indispensable to use microorganisms. Therefore, a method for long-term storage of microorganisms that cannot be artificially cultured has not been developed.
そのため、従来は、人工培養不可能な微生物であって、その微生物が宿主細胞が存在する場合にのみ生育可能である場合は、その宿主細胞を育成して、植え継ぐ必要があった。例えば、うどんこ病菌の病原菌Ｅｒｙｓｉｐｈｅ ｐｏｌｙｇｏｎｉおよびＳｐｈａｅｒｏｔｈｅｃａ ｆｕｌｉｇｉｎｅａ、Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｔｈｉｉ （Ｃａｓｔａｇｎｅ） Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ、Ｏｉｄｉｕｍ ｓｕｂｇｅｎ．Ｐｓｅｕｄｏｉｄｉｕｍ、および、Ｏｉｄｉｕｍ ｓｕｂｇｅｎ． Ｒｅｔｉｃｕｌｏｉｄｉｕｍは、絶対寄生菌であり、生きた植物にしか寄生できない。また、うどんこ病の病原菌については、人工培養系が確立されていない。そのため、うどんこ病の病原菌を各種研究に使用するためには、絶えず生きた宿主植物を育成して、植え継いで行く必要がある。寄生性の比較検討や薬剤耐性菌の検定など、一度に多数の菌株を扱う研究においては、菌を植え継ぐ宿主植物と接種に用いる植物を大量に育成する必要がある。個々の菌株を隔離して植え継ぐことも必要である。うどんこ病のような病原菌を維持するためには、相当の広範なスペースが必要であるとともに、植物と菌株の育成、管理に必要な労力も多大である。また、多数の菌株を扱う研究の期間が長期化すると、菌株相互のコンタミネーションの危険性が増す。そのため、長期間、安全に同一施設で維持することは、ほとんど不可能である。 Therefore, conventionally, when a microorganism that cannot be artificially cultured and can grow only when the host cell exists, the host cell must be grown and planted. For example, the powdery mildew pathogens Erysiphe polygoni and Sphaerotheca furiginea, Podophaera xanthii (Castagne) Braun & Shishoff, Oidium subgen. Pseudoidium and Oidium subgen. Reticuloidium is an absolute parasite and can only infest living plants. In addition, no artificial culture system has been established for the powdery mildew pathogen. Therefore, in order to use the powdery mildew pathogen for various studies, it is necessary to cultivate a live host plant continuously and plant it. In studies dealing with a large number of strains at once, such as comparative examination of parasitic properties and testing for drug-resistant bacteria, it is necessary to grow a large number of host plants to be used for inoculation and plants used for inoculation. It is also necessary to isolate and transfer individual strains. In order to maintain pathogenic bacteria such as powdery mildew, a considerable amount of space is required, and the labor required for the cultivation and management of plants and strains is also great. In addition, as the period of research dealing with a large number of strains increases, the risk of contamination between strains increases. For this reason, it is almost impossible to maintain the same facility safely for a long time.
そのため、人工培養が不可能な微生物の長期保存法の開発が望まれている。 Therefore, it is desired to develop a long-term preservation method for microorganisms that cannot be artificially cultured.
この出願の発明に関連する先行技術文献情報としては、次のものがある。
本発明の課題は、人工培養が不可能な微生物の長期保存方法およびその方法のための組成物を提供することである。 An object of the present invention is to provide a method for long-term preservation of microorganisms that cannot be artificially cultured and a composition for the method.
従来の微生物長期保存方法は、微生物を人工培養する工程を必要とするため、人工培養可能な微生物のみが長期保存の対象であった。本発明者らは、人工培養が不可能である微生物について、その微生物が感染した宿主組織および／またはその微生物が共生した共生体の状態で凍結保存することによる微生物の長期保存法を確立して、本発明を完成した。さらに発明者らは、微生物が感染した宿主組織より微生物の分生子を単離し、その分生子を凍結保存することによる微生物の長期保存法を確立した。 Since conventional microorganism long-term storage methods require a step of artificially culturing microorganisms, only microorganisms that can be artificially cultured have been subject to long-term storage. The present inventors have established a method for long-term preservation of microorganisms by cryopreserving microorganisms that cannot be artificially cultured in a host tissue infected with the microorganisms and / or symbiotic organisms. The present invention has been completed. Furthermore, the inventors have established a method for long-term preservation of microorganisms by isolating conidia of microorganisms from host tissues infected with microorganisms and freezing the conidia.
従来は、微生物の無性世代である分生子や菌糸は、凍結などの過酷な環境では生存し難いと認識されていたので、無性世代を長期保存のために使用することは検討されていなかった。この点においても、本発明は、予想外の効果を奏するものである。 Conventionally, conidia and mycelia, which are asexual generations of microorganisms, were recognized as difficult to survive in harsh environments such as freezing, so the use of asexual generations for long-term storage has not been studied. It was. In this respect as well, the present invention has an unexpected effect.
従って、本発明は、以下を提供する。
（項目１） 微生物を保存する方法であって、以下：
ａ）微生物が感染した宿主生物の組織を乾燥する工程；および
ｂ）該乾燥組織を凍結する工程、
を包含する、方法。
（項目２） 項目１に記載の方法であって、さらに、前記工程（ａ）の前に、微生物が感染した組織を小片にする工程を包含する、方法。
（項目３） 項目２に記載の方法であって、前記微生物が感染した組織を小片にする工程によって調製される小片が、５〜７ｍｍ×５〜１０ｍｍの大きさである、方法。
（項目４） 項目１に記載の方法であって、ここで、前記（ａ）乾燥工程が乾燥剤存在下において室温で３時間以上行われる、方法。
（項目５） 項目４に記載の方法であって、ここで、前記（ａ）乾燥工程が乾燥剤存在下において室温で３時間〜１２時間行われる、方法。
（項目６） 前記乾燥剤が塩化カルシウムである、項目５に記載の方法。
（項目７） 項目１に記載の方法であって、ここで、前記（ｂ）凍結する工程が、以下：（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）液体窒素中で−１６５℃で凍結する工程
からなる群から選択される、方法。
（項目８） 前記微生物が、うどんこ病の病原菌、べと病のべと病菌、さび病のさび病菌、および、菌根菌からなる群から選択され、宿主生物が、該病原菌に感染した植物である、項目１に記載の方法。
（項目９） 前記微生物が、うどんこ病の病原菌であり、宿主生物が、該うどんこ病に感染した植物である、項目１に記載の方法。
（項目１０） 前記微生物が、Ｅｒｙｓｉｐｈｅ ｐｏｌｙｇｏｎｉ、Ｅｒｙｓｉｐｈｅ ｐｉｓｉ、Ｅｒｙｓｉｐｈｅ ｐａｅｏｎｉａｅ、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅｒｕｍ、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅｒｕｍ ｖａｒ．ｃｉｃｈｏｒａｃｅｒｕｍ、Ｓｐｈａｅｒｏｔｈｅｃａ ｐａｎｎｏｓａ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｌｉｇｉｎｅａ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｉｌｉｐｅｎｄｕｌａｅ、Ｓｐｈａｅｒｏｔｈｅｃａ ｂａｌｓａｍｉｎａｅ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｓｃａ、Ｕｎｃｉｎｕｌａ ｓｉｍｕｌａｎｓ、Ｏｉｄｉｕｍ ｂｅｇｏｎｉａｅ ｖａｒ．ｍａｃｒｏｓｐｏｒｕｍ、Ｏｉｄｉｕｍ ｓｐ．、Ｓｐｈａｅｒｏｔｈｅｃａ ｈｕｍｕｌｉ、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｅｒｙｓｉｐｈｅ：ａｃｔｉｎｏｓｔｅｍｍａｔｉｓ Ｂｒａｕｎ［ゴキヅル・ミヤマニガウリうどんこ病］、ａｑｕｉｌｅｇｉａｅ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ａｑｕｉｌｅｇｉａｅ［オダマキうどんこ病］、ａｑｕｉｌｅｇｉａｅ ｖａｒ．ｒａｎｕｎｃｕｌｉ（Ｇｒｅｖｉｌｌｅ）Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［デルヒニュウム・ラナンキュウラス・トリカブト類・アキカラマツうどんこ病］、ｂｒａｕｎｉｉ Ｎｏｍｕｒａ［アサマヒゴタイうどんこ病］、ｂｅｔａｅ（Ｖａｎｈａ）Ｗｅｌｔｚｉｅｎ［アリタソウ・ケアリタソウうどんこ病］、ｃａｕｃａｓｉｃａ Ｓｉｍｏｎｉａｎ ｖａｒ．ｃｏｒｃｈｏｒｉ Ｔａｎｄａ［モロヘイヤうどんこ病］、ｃｅｌｏｓｉａｅ Ｔａｎｄａ［ノゲイトウうどんこ病］、ｃｈｌｏｒａｎｔｈｉ（Ｇｏｌｏｖｉｎ ｅｔ Ｂｕｎｋｉｎａ）Ｂｒａｕｎ［フタリシズカうどんこ病］、ｄｉｅｒｖｉｌｌａｅ Ｍｉｙａｂｅ ｅｘ Ｂｒａｕｎ［ハコネウツギ類うどんこ病］、ｇｌｙｃｉｎｅｓ Ｔａｉ ｅｍｅｎｄ Ｚｈｅｎｇ ｖａｒ．ｇｌｙｃｉｎｅｓ［ヤブマメ・ナツフジ・ヤブハギ類うどんこ病］、ｇｌｙｃｉｎｅｓ ｖａｒ ｌｅｓｐｅｄｅｚａｅ（Ｚｈｅｎｇ ｅｔ Ｂｒａｕｎ）Ｂｒａｕｎ ｅｔ Ｚｈｅｎｇ［ケハギ・メドハギうどんこ病］、ｇｒａｃｉｌｉｓ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ ｖａｒ．ｇｒａｃｉｌｉｓ［アラカシ・シラカシ・ウラジロカシうどんこ病］、ｇｒａｃｉｌｉｓ ｖａｒ．ｌｏｎｇｉｓｓｉｍａ Ｎｏｍｕｒａ［アカガシ・ツクバネガシうどんこ病］、ｈｅｒａｃｌｅｉ ｄｅ Ｃａｎｄｏｌｌｅ［ニンジン・パセリうどんこ病］、ｈｏｍｍａｅ Ｂｒａｕｎ［ナギナタコウジュ・ナミキソウ類うどんこ病］、ｈｕａｙｉｎｅｎｓｉｓ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［ヤマハッカうどんこ病］、ｌｙｔｈｒｉ Ｊｕｎｅｌｌ［ミソハギ・エゾミソハギうどんこ病］、ｍａｃｌｅａｙａｅ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［タケニグサうどんこ病］、ｍａｌｌｏｔｉ Ｃｈｅｎ ｅｔ Ｇａｏ［アカメガシワうどんこ病］、ｍａｙｏｒｉｉ Ｂｌｕｍｅｒ ｖａｒ．ｊａｐｏｎｉｃａ Ｂｒａｕｎ ｅｔ Ｎｏｍｕｒａ［アザミ類うどんこ病］、ｍａｙｏｒｉｉ Ｂｌｕｍｅｒ ｖａｒ．ｍａｙｏｒｉｉ［ノアザミうどんこ病］、ｏｔａｎｉｉ Ｎｏｍｕｒａ［ムカゴイラクサうどんこ病］、ｐａｅｏｎｉａｅ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［シャクヤクうどんこ病］、ｐｉｌｅａｅ Ｂｒａｕｎ［ミズ類うどんこ病］、ｐｉｓｉ ｄｅ Ｃａｎｄｏｌｌｅ［エンドウ・キバナオウギ・クララ・ルピナスうどんこ病］、ｐｌｅｃｔｒａｎｔｈｉ Ｓｈｉｎ ｅｔ Ｌａ［オオヤマハッカうどんこ病］、ｐｏｌｙｇｏｎｉ ｄｅ Ｃａｎｄｏｌｌｅ［ソバ・タデ類うどんこ病］、ｐｕｓｉｌｌａ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［マルバネコノメうどんこ病］、ｒｏｄｇｅｒｉｓｉａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ［ヤグルマソウうどんこ病］、ｓｔｅｐｈａｎｉａｅ Ｔａｎｄａ［タマザキツヅラフジうどんこ病］ ｔａｋａｍａｔｓｕｉ Ｎｏｍｕｒａ［ハスうどんこ病］、ｕｌｍａｒｉａｅ Ｄｅｓｍａｚｉｅｒｅｓ［シモツケソウうどんこ病］、ｖｉｃｉａｅ−ｕｎｉｊｕｇａｅ（Ｈｏｍｍａ）Ｂｒａｕｎ［ナンテンハギうどんこ病］、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｍｉｃｒｏｓｐｈａｅｒａ：ａｂｅｌｉｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アベマキうどんこ病］、ａｋｅｂｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アケビうどんこ病］、ａｌｐｈｉｔｏｉｄｅｓ（Ｇｒｉｆｆｏｎ ｅｔ Ｍａｕｂｌａｎｃ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏｉｄｉｕｍ ｑｕｅｒｃｉｎｕｍ Ｔｈｕｍｅｎ ｖａｒ．ｇｅｍｍｉｐａｒｕｍ Ｆｅｒｒａｒｉｓ、クヌギ・ミズナラ・スダジイうどんこ病］、ｂｅｒｂｅｒｉｄｉｃｏｌａ Ｔａｉ［ホソバヒイラギナンテン・メギうどんこ病］、ａｓｔｒａｇａｌｉ ｄｅ Ｃａｎｄｏｌｌｅ［モメンヅルうどんこ病］、ｂａｕｍｅｌｅｒｉ（Ｍａｇｎｕｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クサフジ・ツルフジバカマ・ヨツバハギうどんこ病］、ｂｅｒｂｅｒｉｄｉｃｏｌａ（Ｆ．Ｌ．Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ホソバヒイラギナンテンうどんこ病］、ｂｅｒｂｅｒｉｄｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ａｓｉａｔｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒロハヘビノボラズ・メギうどんこ病］、ｂｅｒｃｈｅｍｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クマヤナギ類うどんこ病］、ｂｌａｓｔｉ（Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオモジ・クロモジうどんこ病］、ｃａｌｏｃｌａｄｏｐｈｏｒａ（Ａｔｋｉｎｓｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アメリカシロナラうどんこ病］、ｃｌｅｔｈｒａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［リョウブうどんこ病］、ｃｏｒｉａｒｉａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ドクウツギうどんこ病］、ｃｏｒｙｌｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハシバミ・ツノハシバミうどんこ病］、ｄｅｕｔｚｉａｅ（Ｂｕｎｋｉｎａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ウツギうどんこ病］、ｅｒｌａｎｇｓｈａｎｅｎｓｉｓ（Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ミヤマウグイスカグラうどんこ病］、ｆｒｉｅｓｔｉｉ Ｌｅｖｅｉｌｌｅ ｖａｒ．ｄａｈｕｒｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クロツバラ・クロウメモドキうどんこ病］、ｈｅｌｗｉｎｇｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハナイカダうどんこ病］、ｈｏｍｍａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハシバミ類うどんこ病］、ｉｔｏａｎａ（Ｎｏｍｕｒａ， Ｔａｎｄａ ｅｔ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オケラうどんこ病］、ｉｚｕｅｎｓｉｓ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツツジ類うどんこ病］、ｊｕｇｌａｎｄｉｓ（Ｇｏｌｏｖｉｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クルミ・サワグルミうどんこ病］、ｋａｔｕｍｏｔｏｉ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバイボタうどんこ病］、ｌｉｇｕｓｔｒｉ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イボタノキうどんこ病］、ｌｏｎｉｃｅｒａｅ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ｌｏｎｉｃｅｒａｅ［スイカズラうどんこ病］、ｍａｇｎｉｆｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［モクレン・コブシうどんこ病］、ｍａｇｎｏｌｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ホオノキうどんこ病］、ｍａｙｕｍｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［マユミうどんこ病］、ｍｅｌｉｏｓｍａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アワブキうどんこ病］、ｍｅｎｉｓｐｅｒｍｉ Ｈｏｗｅ ｖａｒ．ｄａｈｕｒｉｃａ（Ｂｒａｕｎ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コウモリカズラうどんこ病］、ｍｅｎｉｓｐｅｒｍｉ Ｈｏｗｅ ｖａｒ．ｓｉｎｏｍｅｎｉｉ（Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツヅラフジうどんこ病］、ｍｉｕｒａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［キンギンボク・チシマヒョウタンボクうどんこ病］、ｍｉｙａｂｅａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［エゴノキうどんこ病］、ｍｕｌｔｉａｐｐｅｎｄｉｃｉｓ （Ｚｈａｏ ＆ Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒロハヘビノボラズうどんこ病］、ｎｏｍｕｒａｅ Ｂｒａｕｎ［サワフタギうどんこ病］、ｏｒｉｘａｅ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コクサギうどんこ病］、ｐｅｎｉｃｉｌｌａｔａ（Ｗａｌｌｒｏｔｈ）Ｌｉｎｋ［ケヤマハンノキ・ミヤマハンノキ・ヒメヤシャブシうどんこ病］、ｐｈｙｌｌａｎｔｈｉ（Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コバンノキうどんこ病］、ｐｉｃｒａｓｍａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニガキうどんこ病］、ｐｓｅｕｄｏｌｏｎｉｃｅｒａｅ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオツヅラフジうどんこ病］、ｐｕｌｃｈｒａ（Ｃｏｏｋ ｅｔ Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｐｕｌｃｈｒａ［ハナミズキ・ヤマボウシ、ミズキうどんこ病］、ｒｏｂｉｎｉｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハリエンジュうどんこ病］、ｒｕｓｓｅｌｉｉ（Ｃｌｉｎｔｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カタバミ類うどんこ病］、ｓｃｈｉｚａｎｄｒａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［マツブサうどんこ病］、ｓｅｃｕｒｉｎｅｇａｅ（Ｔａｉ ＆ Ｗｅｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒトツバハギうどんこ病］、ｓｈｉｎａｎｏｅｎｓｉｓ（Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カンボクうどんこ病］、ｖｉｂｕｒｎｉ Ｄｕｂｙ［ガマズミ類うどんこ病］、ｓｔａｐｈｙｌｅａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ミツバウツギうどんこ病］、ｓｕｂｔｒｉｃｈｏｔｏｍａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハリエンジュうどんこ病］、ｓｙｒｉｎｇａｅ−ｊａｐｏｎｉｃａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ライラック類うどんこ病］、ｔｉｌｉａｅ（Ｅｌｉａｄｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバボタイジュうどんこ病］、ｔｒｉｆｏｌｉｉ Ｇｒｅｖｉｌｌｅ ｖａｒ．ｔｒｉｆｏｌｉｉ［アカクローバうどんこ病］、ｖａｎｂｒｕｎｔｉａｎａ Ｇｅｒａｒｄ ｖａｒ．ｓａｍｂｕｃｉ−ｒａｃｅｍｏｓａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニワトコうどんこ病］、ｗａｌｌｒｏｔｈｉｉ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［スノキ類うどんこ病］、ｗｅｉｇｅｌａｅ−ｄｅｃｏｒａｅ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニシキウツギうどんこ病］、ｙａｍａｄａｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ケンポナシうどんこ病］、Ｍｉｃｒｏｓｐｈａｅｒａ ｌｏｎｉｃｅｒａｅ−ｒａｍｏｓｉｓｓｉｍａｅ Ｔａｎｄａ［コウグイスカグラうどんこ病］、ｑｕｅｒｃｉ Ｓａｗａｄａ［ナラ類・ウバメガシうどんこ病］、ｓｃｈｉｚａｎｄｒａｅ Ｓａｗａｄａ ｆ．ｂｒｅｖｉｓｅｔａ Ｔａｎｄａ ＆ Ｎｏｍｕｒａ［チョウセンゴミシうどんこ病］、ｓｉｎｅｎｓｉｓ Ｙｕ［クリうどんこ病］、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｕｎｃｉｎｕｌａ：ａｃｔｉｎｉｄｉａｅ（Ｍｉｙａｂｅ ｅｘ Ｊａｃｚｅｗｓｋｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ａｃｔｉｎｉｄｉａｅ［マタタビ・サルナシうどんこ病］、ａｃｔｉｎｉｄｉａｅ ｖａｒ．ａｒｇｕｔａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サルナシ・マタタビうどんこ病］、ａｄｕｎｃａ（Ｗａｌｌｒｏｔｈ）Ｆｒｉｅｓ ｖａｒ．ａｄｕｎｃａ［バッコヤナギうどんこ病］、ａｄｕｎｃａ ｖａｒ．ｍａｎｄｓｈｕｒｉｃａ（Ｍｉｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヤナギ類うどんこ病］、ａｍａｎ
ｏｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒメヤシャブシうどんこ病］、ａｕｓｔｒａｌｉａｎａ（ＭｃＡｌｐｉｎｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サルスベリうどんこ病］、ｂｅｔｕｌｉｎａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カンバ類うどんこ病］、ｂｉｆｕｒｃａｔａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コナラうどんこ病］、ｂｕｃｋｌｅｙａｅ（Ｎｏｍｕｒａ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツクバネうどんこ病］、ｃａｒｐｉｎｉｃｏｌａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シデ類うどんこ病］、ｃｅｄｒｅｌａｅ（Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チャンチンうどんこ病］、ｃｌａｎｄｅｓｔｉｎａ Ｂｉｖｏｎａ−Ｂｅｒｎａｒｄｉ［ニレ類うどんこ病］、ｃｌｉｎｔｏｎｉｏｐｓｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｃｕｒｖｉｓｐｏｒａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イヌブナうどんこ病］、ｅｈｒｅｔｉａｅ（Ｋｅｉｓｓｌ．）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔａｉｗａｎｅｎｓｉｓ（Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チシャノキうどんこ病］、ｆｒａｘｉｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｈｙｄｒａｎｇｅａｅ（Ｃｈｅｎ ＆ Ｇａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノリウツギうどんこ病］、ｋｕｓａｎｏｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｋｕｓａｎｏｉ［エノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ａｐｈａｎａｎｔｈｅｓ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムクノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ｚｅｌｋｏｗａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ケヤキうどんこ病］、ｌａｔａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニシキウツギうどんこ病］、ｌｊｕｂａｒｓｋｉｉ ｖａｒ．ａｄｕｎｃｏｉｄｅｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオイタヤメイゲツうどんこ病］、ｌｊｕｂａｒｓｋｉｉ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｌｊｕｂａｒｓｋｉｉ［イロハモミジ・ヤマモミジうどんこ病］、ｍａｔｓｕｎａｍｉａｎａ（Ｎｏｍｕｒａ， Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヤマハゼうどんこ病］、ｍｉｙａｂｅｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハンノキ類うどんこ病］、ｍｏｒｉ（Ｍｉｙａｋｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ｎｅｃａｔｏｒ［ブドウうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ａｍｐｅｌｏｐｓｉｄｉｓ（Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノブドウうどんこ病］、ｎｉｓｈｉｄａｎａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｏｌｅｏｓａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｏｌｅｏｓａ［シナノキうどんこ病］、ｏｌｅｏｓａ ｖａｒ．ｚｈｅｎｇｉｉ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバボダイジュうどんこ病］、ｐｉｃｒａｓｍａｅ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニガキうどんこ病］、ｐｓｅｕｄｏｃａｒｐｉｎｉｃｏｌａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サワシバうどんこ病］、ｓａｌｉｃｉ−ｇｒａｃｉｌｉｓｔｙｌａｅ Ｈｏｍｍａ［カワヤナギ・ネコヤナギうどんこ病］、ｓａｌｍｏｎｉｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｓｃｈｉｚｏｐｈｒａｇｍａｔｉｓ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イワガラミうどんこ病］、ｓｅｎｇｏｋｕｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツルウメモドキうどんこ病］、ｓｅｐｔａｔａ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ＊［コナラ・カシワうどんこ病］、ｓｉｍｕｌａｎｓ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｓｉｍｕｌａｎｓ［ノイバラうどんこ病］、ｓｉｍｕｌａｎｓ ｖａｒ．ｔａｎｄａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノイバラうどんこ病］、ｔｏｇａｓｈｉａｎａ ｖａｒ．ｒｉｇｉｄａ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバアサガラうどんこ病］、ｔｏｇａｓｈｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔｏｇａｓｈｉａｎａ［ハクウンボクうどんこ病］、ｖｅｒｎｉｃｉｆｅｒａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ウルシ・ヤマハゼ・ヌルデ・ケムリノキうどんこ病］、ｖｉｂｕｒｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガマズミ類うどんこ病］、Ｕｎｃｉｎｕｌａ ａｓｔｅｒｉｓ Ｓａｗａｄａ［ノコンギクうどんこ病］、ｃａｒｐｉｎｉ−ｃｏｒｄａｔａｅ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［サワシバうどんこ病］、ｍｉｙａｂｅｉ ｖａｒ．ｈｅｒｍａｐｈｒｏｄｉｔｉｃａ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［ヒメヤシャブシうどんこ病］、Ｇｏｌｏｖｉｎｏｍｙｃｅｓ：ａｄｅｎｏｐｈｏｒａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［ツリガネニンジン・ソバナうどんこ病］、ａｒａｂｉｄｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［エゾハタザオうどんこ病］、ａｒｔｅｍｉｓｉａｅ（Ｇｒｅｖｉｌｌｅ）Ｇｅｌｊｕｔａ［ヨモギ・オオヨモギ・オトコヨモギうどんこ病］、ｂｉｏｃｅｌｌａｔｕｓ（Ｅｈｒｅｎｂｅｒｇ）Ｇｅｌｊｕｔａ ｖａｒ．ｍｏｎａｒｄａｅ〔モナルダうどんこ病〕、ｂｒａｕｎｅｏｐｕｎｃｔａｔａ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ミゾホオズキうどんこ病］、ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ［ノコギリソウ類・アザミ類・ヒヨドリバナ類・ハンゴンソウ・ノゲシ・ホオズキなどナス科・キク科植物の多数のうどんこ病］、ｌａｐｏｒｔｅａｅ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ムカゴイラクサうどんこ病］、ｓｏｒｄｉｄｕｓ（Ｊｕｎｅｌｌ）Ｇｅｌｊｕｔａ［オオバコうどんこ病］、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅａｒｕｍ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ｌａｔｉｓｐｏｒａ Ｂｒａｕｎ［ブタクサうどんこ病］、Ｎｅｏｅｒｙｓｉｐｈｅ：ｃｕｍｍｉｎｓｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ［モミジガサ・メタカラコウ・テバコモミジガサうどんこ病］、ｇａｌｅｏｐｓｉｄｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｂｒａｕｎ［オドリコソウ・ジャコウソウ・イヌゴマ・ヤマハッカ・ヒキオコシうどんこ病］、ｇｅｒａｎｉｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ［ゲンノショウコ・チシマフウロ・イチゲフウロ・エゾフウロ・ハクサンフウロうどんこ病］、Ｐｈｙｌｌａｃｔｉｎｉａ：ａｉｌａｎｔｈｉ（Ｇｏｌｏｖｉｎ ｅｔ Ｂｕｎｋｉｎａ）Ｙｕ［ニワウルシうどんこ病］、ａｌｎｉ Ｙｕ ＆ Ｈａｎ［ハンノキ類うどんこ病］、ａｍｐｅｌｏｐｓｉｄｉｓ Ｙｕ ＆ Ｌａｉ［サンカクヅルうどんこ病］、ｂｒｏｕｓｓｏｎｅｔｉａｅ−ｋａｅｍｐｆｅｒｉ Ｓａｗａｄａ［コウゾ裏うどんこ病］、ｆｒａｘｉｎｉ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｆｕｓｓ［トネリコ類裏うどんこ病・マサキうどんこ病］、ｇｕｔｔａｔａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ［ハシバミ類・マンサク・コブシ・マタタビ・裏うどんこ病・カンバ類・ハンノキ類・サワシバ・イヌシデ・フサザクラ・ニガキ・オオバアサガラ・ネジキ・ジャケツイバラうどんこ病］、ｊｕｇｌａｎｄｉｓ Ｔａｏ ＆ Ｑｉｎ［オニクルミ・サワグルミうどんこ病・シナサワグルミ裏うどんこ病］、ｋａｋｉｃｏｌａ Ｓａｗａｄａ［カキうどんこ病］、ｍａｇｎｏｌｉａｅ Ｙｕ ＆ Ｌａｉ［コブシ類裏うどんこ病］、ｍａｌｉ（Ｄｕｂｙ）Ｂｒａｕｎ［ナシ・セイヨウナシうどんこ病］、ｍｏｒｉｃｏｌａ（Ｈｅｎｎｉｎｇｓ）Ｈｏｍｍａ［クワ裏うどんこ病］、ｐｔｅｒｏｓｔｙｒａｃｉｓ Ｙｕ ＆ Ｌａｉ［オオバアサガラ裏うどんこ病］、ｒｏｂｏｒｉｓ（Ｇａｃｈｅｔ）Ｂｌｕｍｅｒ［クリ・アラカシ裏うどんこ病］、ｓａｌｍｏｎｉｉ Ｂｌｕｍｅｒ［キリうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｐｏｄｏｓｐｈａｅｒａ：ｃｅｒｃｉｄｉｐｈｙｌｌｉ Ｔａｎｄａ ＆ Ｎｏｍｕｒａ［カツラうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ａｕｃｕｐａｒｉａｅ（Ｅｒｉｋｓｓｏｎ）Ｂｒａｕｎ［ナナカマドうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ｃｌａｄｅｓｔｉｎａ［シモツケ類うどんこ病］、ｃｕｒｖｉｓｐｏｒａ Ｎｏｍｕｒａ［ナナカマドうどんこ病］、ｌｅｕｃｏｔｒｉｃｈａ（Ｅｌｌｉｓ ＆ Ｅｖｅｒｈａｒｔ）Ｓａｌｍｏｎ ｖａｒ．ｌｅｕｃｏｔｒｉｃｈａ［不完全世代：Ｏｉｄｉｕｍ ｆａｒｉｎｏｓｕｍ Ｃｏｏｋｅ、リンゴ・ズミうどんこ病］、ｌｏｎｇｉｓｅｔａ Ｓａｗａｄａ［ウワミズザクラ・ザイフリボクうどんこ病］、ｍｙｒｔｉｌｌｉｎａ（Ｓｃｈｂ．Ｆｒ）Ｋｕｎｚｅ ｖａｒ．ｍｙｒｔｉｌｌｉｎａ［クロウスゴうどんこ病］、ｓｐｉｒａｌｉｓ Ｍｉｙａｂｅ［ハルニレうどんこ病］、ｔｒｉｄａｃｔｙｌａ（Ｗａｌｌｒｏｔｈ）ｄｅ Ｂａｒｙ ｖａｒ．ｔｒｉｄａｃｔｙｌａ［不完全世代：Ｏｉｄｉｕｍ ｐａｓｓｅｒｉｎｉｉ Ｂｅｒｔｏｌｏｎｉ、モモ・スモモ・アンズ・ウメ・ユスラウメ・ニワウメ・サクラうどんこ病］、ｖｉｂｕｒｎｉ Ｂｒａｕｎ［ガマズミう類どんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｐ．［カマツカうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｓｐｈａｅｒｏｔｈｅｃａ：ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ａｐｈａｎｉｓ［不完全世代：Ｏｉｄｉｕｍ ｒｕｂｏｒｕｍ Ｒａｂｅｎｈｏｒｓｔ、イチゴうどんこ病］、ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ｖａｒ．ｈｙａｌｉｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［キジムシロうどんこ病］、ａｓｔｉｌｂｉｃｏｌａ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカショウマ・トリアシショウマうどんこ病］、ｂａｌｓａｍｉｎａｅ（Ｋａｒｉ ｅｘ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏｉｄｉｕｍ ｂａｌｓａｍｉｎａｅ Ｒａｊｄｅｒｋａｒ、ホウセンカ・キツリフネ・ツリフネソウうどんこ病］、ｃａｌｌｉｃａｒｐａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムラサキシキブうどんこ病］、ｃａｒｄａｍｉｎｅｓ Ｎｏｍｕｒａ［タネツケバナうどんこ病］、ｃａｒｉｃａｅ−ｐａｐａｙａｅ（Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｃａｒｉｃａｅ Ｎｏａｃｋ、パパイヤうどんこ病］、ｃｕｃｕｒｂｉｔａｅ（Ｊａｃｚｅｗｓｋｉｉ）Ｚｈａｏ［キュウリ・カボチャ・セイヨウカボチャ・ヒョウタンのうどんこ病］、ｄｉｃｌｉｐｔｅｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハグロソウうどんこ病］、ｅｐｉｌｏｂｉｉ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカバナ類うどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｅｌｉｏｓｃｏｐｉａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トウダイグサうどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｉｒｔａｅ（Ｂｒａｕｎ ＆ Ｓｏｍａｎｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［エノキグサうどんこ病］、ｆｅｒｒｕｇｉｎｅａ ｖａｒ．ａｌｂｉｆｌｏｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シロバナトウウチソウうどんこ病］、ｆｅｒｒｕｇｉｎｅａ（Ｓｃｈｌｅｃｈｔｅｎｄａｈｌ ｅｘ Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｆｅｒｒｕｇｉｎｅａ［ワレモコウうどんこ病］、ｆｕｇａｘ（Ｐｅｎｚｉｇ
＆ Ｓａｃｃａｒｄ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ゲンノショウコうどんこ病］、ｆｕｓｃａ（Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［不完全世代：Ｏ．ｃｉｔｒｕｌｌｉ Ｙｅｎ ＆ Ｗａｎｇ、キュウリ・カボチャ・ユウガオ・トウガン・ゴボウ・フキ・コスモス・ヒマワリ・キクイモ・ペチュニア・オオキンケイギク・シオン・ダリア・リュウゼツサイ・ルドベキアうどんこ病］、ｈｉｂｉｓｃｉｃｏｌａ Ｚｈａｏ［フヨウうどんこ病］、ｉｎｔｅｒｍｅｄｉａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クサギうどんこ病］、ｍｏｒｓ−ｕｖａｅ（Ｓｃｈｗｅｉｎｉｔｚ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［スグリ類うどんこ病］、ｐａｎｎｏｓａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）ｄｅ Ｂａｒｙ［不完全世代：Ｏ．ｌｅｕｃｏｃｏｎｉｕｍ Ｄｅｓｍａｚｉｅｒｅｓ， バラ・ハマナスうどんこ病］、ｐｈａｓｅｏｌｉ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アズキ・ヤブツルアズキうどんこ病］、ｐｓｅｕｄｏｆｕｓｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワクサうどんこ病］、ｓｉｂｉｒｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クガイソウ・コシオガマうどんこ病］、ｓｐａｒｓａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガガイモ・オオカモメズルうどんこ病］、ｓｐｉｒａｅａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｂｏｔｒｙｏｉｄｅｓ（Ｃｏｒｄａ）Ｃｅｓａｔｉ、シジミバナ・ユキヤナギ・オニシモツケうどんこ病］、ｓｔｅｐｈａｎａｎｄｒａｅ（Ｊａｃｚｅｗｓｋｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コゴメウツギうどんこ病］、ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［ウリ類・ヒマワリ・ヒャクニチソウ・フキ・ゴボウ・アメリカセンダングサ・キンセンカ・ベニバナ・アキノノゲシ・ヤマニガナ・ガーベラ・フヨウ・ナス・ヤブガラシ・エノキグサなど多数のうどんこ病］、Ｃｙｓｔｏｔｈｅｃａ：ｗｒｉｇｈｔｉｉ Ｂｅｒｋｅｌｅｙ ＆ Ｃｕｒｔｉｓ［カシ類紫かび病］、ｌａｎｅｓｔｒｉｓ（Ｈａｒｋｎｅｓｓ）Ｍｉｙａｂｅ［ナラ類紫かび病］、Ｔｙｐｈｕｌｏｃｈａｅｔａ：ｊａｐｏｎｉｃａ Ｉｔｏ ＆ Ｈａｒａ［ナラガシワ・ミズナラ・コナラ・トネリコうどんこ病］、Ｓａｗａｄａｅａ：ｂｉｃｏｒｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｍｉｙａｂｅ［カエデ類うどんこ病］、ｐｏｌｙｆｉｄａ（Ｗｅｉ）Ｚｈｅｎｇ ＆ Ｃｈｅｎ ｖａｒ．ｊａｐｏｎｉｃａ Ｂｒａｕｎ ＆ Ｔａｎｄａ［モミジ類・カエデ類うどんこ病］、ｔｕｌａｓｎｅｉ（Ｆｕｃｋｅｌ）Ｈｏｍｍａ［カエデ類うどんこ病］、ｚｈｅｎｇｉｉ Ｎｏｍｕｒａ［エンコウカエデうどんこ病］、Ｂｌｕｍｅｒｉａ：ｇｒａｍｉｎｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｓｐｅｅｒ［ムギ類うどんこ病］、Ｐｌｅｏｃｈａｅｔａ：ｓｈｉｒａｉａｎａ（Ｈｅｎｎｉｎｇｓ）Ｋｉｍｂｒｏｕｇｈ ＆ Ｋｏｒｆ［エノキ・ムクノキうどんこ病］、Ｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｓｅｕｄｏｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｌａｓｍｏｐａｒａ ｓｐｐ．、Ｂｒｅｍｉａ ｓｐｐ．、Ｂｒｅｍｉｅｌｌａ ｓｐｐ．、Ｃｒｏｎａｒｔｉｕｍ ｓｐｐ．、Ｍｅｌａｍｐｓｏｒａ ｓｐｐ．、Ｐｈａｋｏｐｓｏｒａ ｓｐｐ．、Ｕｒｅｄｉｎｏｐｓｉｓ ｓｐｐ．、Ｂｌａｓｔｏｓｐｏｒａ ｓｐｐ．、Ｇｙｍｎｏｓｐｏｒａｎｎｇｉｕｍ ｓｐｐ．、Ｐｕｃｃｉｎｉａ ｓｐｐ．、Ｕｒｏｍｙｃｅｓ ｓｐｐ．、Ａｅｃｉｄｉｕｍ ｓｐｐ．、Ｃａｅｍａ ｓｐｐ．、Ｐｅｒｉｄｅｒｍｉｕｍ ｓｐｐ．、Ｕｒｅｄｏ ｓｐｐ．、Ｐｈｒａｇｍｉｄｉｕｍ ｓｐｐ．、Ｓｔｅｒｅｏｓｔｒａｔｕｍ ｓｐｐ．、Ｔｒｉｃｈｏｌｏｍａ ｍａｔｓｕｔａｋｅ（Ｓ．Ｉｔｏ ｅｔ Ｉｍａｉ）Ｓｉｎｇ．、Ｔｕｂｅｒ ｓｐｐ．、Ｓｕｉｌｌｕｓ ｂｏｖｉｎｅｓ、Ｒｈｉｚｏｐｏｇｏｎ ｒｕｂｅｓｃｅｎｓ、および、ＶＡ菌根からなる群から選択される、項目８に記載の方法。
（項目１１） 前記微生物が、Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｈｔｉｉ （Ｃａｓｔａｇｎｅ） Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ、Ｏｉｄｉｕｍ ｓｕｂｇｅｎ． Ｒｅｔｉｃｕｌｏｉｄｉｕｍ、Ｇｏｌｏｖｉｎｏｍｙｃｅｓ ｓｐｐ．、Ｏｉｄｉｕｍ ｓｕｂｇｅｎ． Ｐｓｅｕｄｏｉｄｉｕｍ、および、Ｅｒｙｓｉｐｈｅ ｓｐｐ．からなる群から選択される、項目１０に記載の方法。
（項目１２） 前記宿主生物が、オシロイバナ、スイートピー、シャクヤク、ケシ類ホウズキ、シオン、キク、バラ、ガーベラ、シネラリア、キョウカノコ、ホウセンカ、ダリア、ノイバラ、ベゴニア、ペチュニア、宿根カスミソウ、カエデ類、アラカシ、シラカシ、マテバシイ、コナラ、サクラ類、サルスベリ、ハナカイドウ、ハナミズキ、モミジ類、ヤナギ類、アオキ、ヒイラギナンテン、サルスベリ、イチゴ、および、キュウリからなる群から選択される、項目９に記載の方法。
（項目１３） 前記宿主生物が、キュウリである、項目１２に記載の方法。
（項目１４） 項目１に記載の（ａ）乾燥工程、および（ｂ）凍結工程によって得られた凍結組織。
（項目１５） 微生物を保存する方法であって、ここで、該微生物は、共生者と共生しており、該方法は、以下：
ａ）該微生物とその該共生者との該共生体を、乾燥する工程；および
ｂ）上記工程（ａ）の乾燥物を凍結する工程、
を包含する、方法。
（項目１６） 前記共生が、相利共生、片利共生、および寄生からなる群から選択される、項目１５に記載の方法。
（項目１７） 項目１５に記載の方法であって、さらに、前記工程（ａ）の前に、共生者の組織を小片にする工程を包含する、方法。
（項目１８） 項目１７に記載の方法であって、前記共生者の組織を小片にする工程によって調製される小片が、５〜７ｍｍ×５〜１０ｍｍの大きさである、方法。
（項目１９） 項目１５に記載の方法であって、ここで、前記（ａ）乾燥工程が乾燥剤存在下において室温で３時間以上行われる、方法。
（項目２０） 項目１９に記載の方法であって、ここで、前記（ａ）乾燥工程が乾燥剤存在下において室温で３時間〜１２時間行われる、方法。
（項目２１） 項目２０に記載の方法であって、ここで、前記乾燥剤が塩化カルシウムである、方法。
（項目２２） 項目１５に記載の方法であって、ここで、前記（ｂ）凍結する工程が、以下：
（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）液体窒素中で−１６５℃で凍結する工程
からなる群から選択される、方法。
（項目２３） 項目１５に記載の（ａ）乾燥工程、および（ｂ）凍結工程によって得られた凍結共生体。
（項目２４）微生物を保存する方法であって、以下：
ａ）微生物が感染した宿主生物から分生子を採集する工程；
ｂ）該分生子を乾燥する工程；および
ｃ）該分生子を凍結する工程、
を包含する、方法。
Accordingly, the present invention provides the following.
(Item 1) A method for preserving microorganisms, the following:
a) drying the tissue of the host organism infected by the microorganism; and
b) freezing the dried tissue;
Including the method.
(Item 2) The method according to Item 1, further comprising a step of making a tissue infected with a microorganism into small pieces before the step (a).
(Item 3) The method according to Item 2, wherein the small piece prepared by the step of making the tissue infected with the microorganism into a small piece has a size of 5 to 7 mm x 5 to 10 mm.
(Item 4) The method according to Item 1, wherein (a) the drying step is performed at room temperature in the presence of a desiccant for 3 hours or more.
(Item 5) The method according to Item 4, wherein (a) the drying step is performed at room temperature in the presence of a desiccant for 3 hours to 12 hours.
(Item 6) The method according to item 5, wherein the desiccant is calcium chloride.
(Item 7) The method according to Item 1, wherein the step (b) of freezing is as follows: (i) after pre-freezing at -70 ° C to -80 ° C, in liquid nitrogen -165 Freezing at ° C; and
(Ii) freezing at −165 ° C. in liquid nitrogen
A method selected from the group consisting of:
(Item 8) A plant in which the microorganism is selected from the group consisting of powdery mildew pathogen, downy mildew fungus, rust rust fungus, and mycorrhizal fungus, and the host organism is infected with the pathogenic fungus The method according to item 1, wherein
(Item 9) The method according to Item 1, wherein the microorganism is a powdery mildew pathogen and the host organism is a plant infected with the powdery mildew.
(Item 10) The microorganism may be Erysiphe polygoni, Erysiphe pisi, Erysiphe paeoniae, Erysiphe cichocererum, Erysiphe cichoracerum var. cichoracerum, sphaerotheca pannosa, sphaerotheca furiginea, sphaerotheca filipendulae, sphaerotheca balsamine bumamine, sphaerotheca fuscaulc. macrosporum, Oidium sp. , Sphaerotheca humuli, Erysiphe section Erysiphe: actinostematis Braun [Gokiru Miyamanigauri powdery mildew], aquilegiae de Candole var. aquilegiae [Audamaki powdery mildew], aquilegiae var. ranunculi (Greville) Zheng et Chen [Derhinium ranunculus, aconite, Akikara pine powdery mildew], braunii Nomura [Asahi goitou powdery mildew], betae (Vanha) Weltzien vice . corchori Tanda [Morohaya powdery mildew], celosiae Tanda [Nogateto powdery mildew], chloranthi (Golovin et Bunkina) Braun [Futarisuka powdery gourd disease] glycines [Yabamame, Natsufuji, Yabuhagi powdery mildew], glycines var lespedezae (Zheng et Braun) Braun et Zheng [Kehagigi Medhagi powdery mildew], gracilis Zhengvet gracilis [Aracasi, Shirakasi, Vulgaris mildew], gracilis var. longisima Nomura [red-eyed tsukubagasashi powdery mildew], heraclei de Candole [carrot, parsley powdery mildew], hommae Braun [naginata-jutsu-namiki-shon-powder-disease], huainensis Misohagi, Ezomisohagi powdery mildew], macleayae Zheng et Chen [takenigusa powdery mildew], malloti Chen et Gao [Akamegasiwa powdery mildew], mayorii Blumer var. japonica Braun et Nomura [thistle powdery mildew], mayorii Blumer var. mayorii [Noasami powdery mildew], otanii Nomura [Mungougoura powdery mildew], paeoniae Zheng et Chen [Peonies powdery mildew], pileae Braun [Mizudo powdery mildew], pile basil Powdery mildew], plectranthi Shin et La [Prunus serrata powdery mildew], polygoni de Candole [soba-tade powdery mildew], psilla Tanda et Nomura [Mulban conodium powdery mildew], rodgerisula Disease], stefaniae Tanda [Tamazakitsu rafuji powdery mildew] takamatsusui Nomur [Lotus powdery mildew], ulmariae Desmazeres [Pleurotus powdery mildew], Biciae-unijugae (Homma) Braun [Nantenhagi powdery mildew] & Takamatsu [Akebi powdery mildew], alphaitoides (Griffon et Maublanc) Braun & Takamatsu [Incomplete generation: Oidium quarcinum Thumen var. gemmiparam Ferraris, Kunugi Mizunara Sudazii powdery mildew], berberidicola Tai [Hobas holly guinea tenge powdery mildew], astragali de Candole Disease], berberidicola (F.L. Tai) Braun & Takamatsu [mushroom powdery mildew], berberidis (de Candole) Level var. asiatica (Braun) Braun & Takamasu [Hiroha snake noboraz barberry powdery mildew], berchemiae (Sawada) Braun & Takamatu [Black-bellied powdery mildew], blasti (Tai) Braun ) Braun & Takamatsu [American white powdery mildew], clethrae (Braun) Braun & Takamatsu [Rybu powdery mildew], coriarie (Nomura) Braun & Takamatsu [Dokutsugi] Disease], de tziae (Bunkina) Braun & Takamatsu [Utsugi powdery mildew], erlangshanensis (Yu) Braun & Takamatsu [Miyama nightingale Kagura powdery mildew], friestii Leveille var. dahurica (Braun) Braun & Takamatsu [black rose rose buckthorn powdery mildew], helwiniae (Sawada) Braun & Takamatsu [Hanaika powdery mildew], hommae (Braun) et Braun) Braun & Takamatsu [Okella powdery mildew], izuensis (Nomura) Braun & Takamatsu [Azalea powdery mildew], juglandis (Golovin) Braun & Takamasu disease Takamatsu [Popular powdery mildew] , Ligustri (Homma) Braun & Takamatsu [privet powdery mildew], lonicerae de Candolle var. lonicerae [honeysuckle powdery mildew], magnifica (Braun) Braun & Takamatsu [mokuren kobushi powdery mildew], magnoliae (Sawada) Braun & Takamasu mum ] Meliosmae (Tanda & Nomura) Braun & Takamatsu [Awabuki powdery mildew], menispermi Howe var. dahurica (Braun & Nomura) Braun & Takamatsu [Bats powdery mildew], menispermi Howe var. Sinomenii (Yu) Braun & Takamasu [Tsurafuji powdery mildew]; Braun & Takamatsu [Hiroha snake noboraz powdery mildew], nomurae Braun [Sawafutagi powdery mildew], orixae (Braun & Tanda) Braun & Takamatu ], Phyllant hi (Tanda & Braun) Braun & Takamatsu [Pinpodium powdery mildew], picrasmae (Sawada) Braun & Takamatsu [Nigaki powdery mildew], pseudorucera (Salmon) Braun & Braun Braun & Takamatsu var. pulchra (dogwood, wild bovine, dogwood powdery mildew), robinicola Braun & Takamatsu (harienju powdery mildew), russerii (Clinton) Braun & Takamatsu (skinny powdery mildew) ], Securinegae (Tai & Wei) Braun & Takamatsu [Popular powdery mildew], Shinanoensis (Tanda) Braun & Takamatsu [Pumbo powdery], viburni Duby's disease Honeybee powdery mildew], s Ubrichochoma (Braun) Braun & Takamatsu [Harienju powdery mildew], syringae-japonicae (Braun) Braun & Takamatu [Lilac powdery mildew], tiliae (Eliade) Braun disease. trifolii [red clover powdery mildew], vanbruntiana Gerard var. Sambuci-racemosae (Braun) Braun & Takamatsu [Elderberry powdery mildew]; Yamadai (Salmon) Braun & Takamatsu [Kemponashi powdery mildew], Microsphaera lonicerae-Romasissimae Tanda [Kouisukagura powdery mildew], Querci Sawada brevista Tanda & Nomura [Chrysanthemum powdery mildew], sinensis Yu [Chestnut powdery mildew], Erysiphe section Uncinula: actinidiae (Miyabe ex Jaczewski) Braun & Tamura. actinidiae [Matatabi / sarnashi powdery mildew], actinidiae var. argutae (Nomura) Braun & Takamatsu [Sarnashi Matatabi powdery mildew], adunaca (Wallroth) Fries var. adunca [Bacco willow powdery mildew], adunca var. mandhurica (Miura) Braun & Takamatsu [willow powdery mildew], aman
oi (Nomura) Braun & Takamatsu [Popular powdery mildew], australiana (McAlpine) Braun & Takamatsu [Umbrella powder]; Disease], buckleyae (Nomura Tanda) Braun & Takamatsu [tsukubane powdery mildew], carpinicola (Hara) Braun & Takamatsu [sideworm powdery mildew], cedrelae (Tai) Braun & tak -Bernardi [D Kind powdery mildew], clintoniopsis (Zheng & Chen) Braun & Takamatsu [Sterculiaceae powdery mildew], curvispora (Hara) Braun & Takamatsu [Fagus japonica powdery mildew], ehretiae (Keissl.) Braun & Takamatsu var. taiwanensis (Tanda) Braun & Takamatu [Chishanoki powdery mildew], fraxinicola Braun & Takamasu [green potato powdery], hydrangeae (Chen & Gao) Braun & Takao. . Kusanoi [Enoki powdery mildew], Kusanoi var. aphananthes (Braun) Braun & Takamatsu [Mukunoki powdery mildew], kusanoi var. zelkowae (Hennings) Braun & Takamatsu [keyberry powdery mildew], lata (Nomura & Tanda) Braun & Takamatsu [Nishikitsugi powdery mildew], ljubarskii var. aduncoides (Zheng & Chen) Braun & Takamatsu [Ooitama Meigetsu powdery mildew], ljubarskii Braun & Takamatsu var. ljubarskii [Bloem & Bun], Matsunamiana (Nomura, Tanda & Braun), Braun & Takamatsu [Yamahose powdery mildew], Miyabei [Mulberry powdery mildew], necker Schweintz var. necator [grape powdery mildew], necator Schweintz var. ampelopsis (Peck) Braun & Takamatsu [no grape powdery mildew], nisidana (Homma) Braun & Takamatsu [Aogiri powdery mildew], oleosa (Zheng & Chen) Braun & Takam. oleosa [linden powdery mildew], oleosa var. zhengii (Braun) Braun & Takamatsu [Popular powdery mildew], picrasmae (Homma) Braun & Takamatsu [Nigaki powdery mildew], pseudocarpinicola (Nomura). River willow, cat willow powdery mildew], salmonii (Sydow) Braun & Takamatu [ash powdery mildew], schizophragmatis (Tanda & Nomura) Braun & Takamasu Key powdery mildew], septata (Salmon) Braun & Takamatsu * [Quercus Kashiwa powdery mildew], simulans (Salmon) Braun & Takamatsu var. simulans [Neubara powdery mildew], simulans var. tandae (Braun) Braun & Takamatsu [Neubara powdery mildew], togashiana var. rigida (Braun & Tanda) Braun & Takamatsu [Plump powdery mildew], togashiana (Braun) Braun & Takamatsu var. Togashiana [Hakuunboku powdery mildew], verniciferae (Hennings) Braun & Takamatsu [Urushi yamahaze, Nurde-Kemlino powdery mildew], viburnicola Braun & Tamamatsuu carpini-cordatae Tanda et Nomura [Sawasiba powdery mildew], Miyabei var. Hermaphroditica Tanda et Nomura [Hymeya Shabushi powdery mildew], Golovinomyces: adenophora (Zheng & Chen) Geljuta [Turiganinjin sobana powdery mildew]・ Two Artemisia, Paramecium powdery mildew], biocellatus (Ehrenberg) Geljuta var. monadae (monalda powdery mildew), brauneopuncta (Braun) Geljuta [Mizohozuki powdery mildew], cichoracerum (de Candole) Geljuta [achilleas, thistles, hydrangeas Powdery mildew], laporteae (Braun) Geljuta [Plumpy powdery mildew], sordidus (Junell) Geljuta [Plumpy powdery mildew], Erysiphe cichoracerum de Candole var. latispora Braun [Ragweed powdery mildew], Neoerysiphe: cumminsiana (Braun) Braun [Momigasa, Metakarako, Tebakomomigusa powdery mildew], galeopsidis (de Candole) geranii (Nomura) Braun [Gennoshouko, Chishimafuuro, Ichigefuuro, Ezofuuro, Hakusanfu powdery mildew], Phyllactinia: ailanthii (Golovin et Bunkina) Yu [Niwaurushi] & Lai [Sankakudon powdery mildew], br oussonetiae-kaempferi Sawada [Urban powdery mildew], fraxini (de Candole) Fuss [Glass powdery mildew / Masaki powdery mildew], gutata (Wallroth: Fries) Powdery mildew, birch, alder, sawnhorn, Insode, Fusazakura, Nigaki, Oobaasagara, Neki, Jukutsuibara powdery mildew, juglandis Tao & Qin [Oyster powdery mildew], magnoliae Yu & Lai [Kobushi back powdery mildew], mary (Duby) Braun [Nasis, pear udon] Disease], moricola (Hennings) Homma [back mulberry powdery mildew], pterostyracis Yu & Lai [Obaasagara back powdery mildew], roboris (Gachet) Blumer [chestnut arakashi back powdery mildew], salmoni ], Posphaera section Podophaera: cercidylphyli Tanda & Nomura [Katsura powdery mildew], cladestina (Wallroth: Fries) Level bar. aucupariae (Eriksson) Braun [Nanakamado powdery mildew], cladestina (Wallroth: Fries) Levelile var. cladestina [Pleurotus mushroom powdery mildew], curvispoora Nomura [rowan powdery mildew], leukotricha (Ellis & Everhart) Salmon var. leucotrica [incomplete generation: Oidium farinosum Cooke, apple / sumi powdery mildew], longiseta Sawada [waiwazuzakura zifriboku powdery mildew], myrtillina (Schb. Fr) Kunze var. myrtillina [Crousgo powdery mildew], spiralis Miyabe [harunile powdery mildew], tridactyla (Wallilloth) de Barry var. tridactyla [incomplete generation: Oidium passeriniii Bertononi, peach, peach, apricot, ume, eusloume, niwaume, cherry powdery mildew], viburni Braun [burdock powdery mildew], Podosphaera sp. [Kamatsuka powdery mildew], Podophaera section Sphaerotheca: aphanis (Wallillo) Braun & Takamatsu var. aphanis [incomplete generation: Oidium ruborum Rabenhorst, strawberry powdery mildew], aphanis (Wallilloth) Braun var. hyalina (Braun) Braun & Takamatsu [Kojimushi white powdery mildew], astilbicola (Zhao) Braun & Takamatsu [Red-skinned mumps], balsaminoe (Kari ex Braun) Spinach, tsutsurifune, tsutsufunesou powdery mildew], callicarpae (Tanda & Nomura) Braun & Takamatsu [purple powdery mildew], cardamines Nomura [rae] : O. caricae Noack, papaya powdery mildew], cucurbitae (Jaczewskii) Zhao [cucumber, pumpkin, pumpkin, leopard powdery mildew], diclipterae (Nomura) Braun & Tamagrospo [Red-bellied powdery mildew], euphorbiae-helioscopiae (Tanda & Nomura) Braun & Takamasu [euphorbia powdery mildew], euphorbiae-hirtae (Braun & Soma). albiflorae (Nomura) Braun & Takamatsu [powdery mildew powdery mildew], ferruginea (Schletchendahl ex Fries) Braun & Takamatsu var. ferruginea (pox powdery mildew), fugax (Penzig
& Saccard) Braun & Takamatsu [geno-shoko powdery mildew], fusca (Fries) Braun & Shishoff [Incomplete generation: O.D. citrulli Yen & Wang, cucumber, pumpkin, yugao, tougan, burdock, burdock, cosmos, sunflower, chrysanthemum, petunia, pachytia, zion, dahlia, ryuzesai, rudbeckia powdery mildew, hibiscicola Braun) Braun & Takamatsu [Wagashi powdery mildew], mors-uvae (Schweinitz) Braun & Takamatsu [currant powdery mildew], pannosa (Wallroth: Fries) de Bary [Incomplete generation: O. leucoconium Desmazeres, Rose Hamanus powdery mildew], phaseoli (Zhao) Braun & Takamatsu [Azuki, Abutsuki Azuki powdery mildew], pseudofusca (Braun) braun & taku・ Koshio Gama powdery mildew], sparsa (Braun) Braun & Takamatsu [Gaigamo okamuzuru powdery mildew], spiraea (Sawada) Braun & Takamatsu [Incomplete generation: O. botryoides (Corda) Cesati, rainbow trout, snowy canopy, powdery mildews Numerous powdery mildews such as calendula, safflower, akinonogeshi, yamanana, gerbera, buoy, eggplant, sorghum, enokigusa], Cytotheca: wrighti Berkeley & Curtis [purple mold fungus], lanestris H Disease], Typhlochaeta: japonica to & Hara [Quercus Aliena, Japanese oak, Quercus, ash powdery mildew], Sawadaea: bicornis (Wallroth) Miyabe [maple such powdery mildew], polyfida (Wei) Zheng & Chen var. japonica Braun & Tanda [momiji, maple powdery mildew], tulasnei (Fuckel) Homma (maple powdery mildew), zhengii Nomura [enkookae powdery mildew], Blumeria: praminisum Disease], Pleochaeta: shiraiana (Hennings) Kimbrough & Korf [Enoki Mukino powdery mildew], Peronospora spp. , Pseudoperonospora spp. Plasmopara spp. Bremia spp. Bremiella spp. Cronartium spp. Melampsora spp. Phakopsora spp. Uredinopsis spp. Blastosporia spp. Gymnosporangium spp. , Puccinia spp. Uromyces spp. , Aecidium spp. Caema spp. , Perdermium spp. , Uredo spp. Phragmidium spp. , Stereostratum spp. , Tricholoma matsutake (S. Ito et Imai) Sing. , Tuber spp. 9. The method of item 8, wherein the method is selected from the group consisting of: Silllus bovines, Rhizopogon rubescens, and VA mycorrhiza.
(Item 11) The microorganism may be Podsphaera xanhti (Castagne) Braun & Shishoff, Oidium subgen. Reticuloidium, Golovinomyces spp. Oidium subgen. Pseudoidium and Erysiphe spp. The method according to item 10, wherein the method is selected from the group consisting of:
(Item 12) The host organisms are oysters, sweet peas, peonies, poppy physalis, zion, chrysanthemum, roses, gerberas, cineraria, mushrooms, spinach, dahlia, neubara, begonia, petunia, perennial gypsophila, maple, arakashi, deer 10. The method according to item 9, wherein the method is selected from the group consisting of: Matebashii, Quercus, Cherry, Crape myrtle, Hanakaidou, Dogwood, Maple, Willow, Aoki, Holly nanten, Crape myrtle, Strawberry, and Cucumber.
(Item 13) The method according to item 12, wherein the host organism is cucumber.
(Item 14) A frozen tissue obtained by (a) a drying step and (b) a freezing step according to Item 1.
(Item 15) A method for preserving a microorganism, wherein the microorganism is symbiotic with a symbiotic person, the method comprising:
a) drying the symbiosis of the microorganism and its symbiosis; and
b) a step of freezing the dried product in the step (a),
Including the method.
(Item 16) The method according to item 15, wherein the symbiosis is selected from the group consisting of mutualism, commensalism, and parasitism.
(Item 17) The method according to item 15, further comprising a step of cutting the symbiotic tissue into pieces before the step (a).
(Item 18) The method according to item 17, wherein the small piece prepared by the step of making the symbiotic tissue into small pieces has a size of 5 to 7 mm x 5 to 10 mm.
(Item 19) The method according to Item 15, wherein (a) the drying step is performed at room temperature in the presence of a desiccant for 3 hours or more.
(Item 20) The method according to item 19, wherein (a) the drying step is performed at room temperature in the presence of a desiccant for 3 hours to 12 hours.
(Item 21) The method according to item 20, wherein the desiccant is calcium chloride.
(Item 22) The method according to Item 15, wherein (b) the step of freezing is as follows:
(I) pre-freezing at -70 ° C to -80 ° C and then freezing at -165 ° C in liquid nitrogen; and
(Ii) freezing at −165 ° C. in liquid nitrogen
A method selected from the group consisting of:
(Item 23) A frozen symbiosis obtained by (a) a drying step and (b) a freezing step according to item 15.
(Item 24) A method for preserving microorganisms, comprising:
a) collecting conidia from host organisms infected with microorganisms;
b) drying the conidia; and
c) freezing the conidia;
Including the method.
本発明によって、従来長期保存が不可能であった微生物を長期保存することが可能になる。 According to the present invention, it is possible to store microorganisms that could not be stored for a long period of time.
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
本明細書において使用する場合、用語「微生物」とは、微小であり、肉眼では観察できないよいうな生物をいう。微生物としては、例えば、ウイルス、細菌、真菌（カビおよび酵母を含む）、単細胞植物、単細胞動物を包含するが、これらに限定されない。本発明における微生物は、好ましくは細菌および真菌、より好ましくは真菌、さらにより好ましくはカビである。 As used herein, the term “microorganism” refers to a good organism that is microscopic and cannot be observed with the naked eye. Examples of microorganisms include, but are not limited to, viruses, bacteria, fungi (including mold and yeast), unicellular plants, and unicellular animals. The microorganisms in the present invention are preferably bacteria and fungi, more preferably fungi, and even more preferably mold.
本明細書において使用する場合、用語「カビ」とは、真菌類に属する糸状菌をいい、生活環の中で、分生子および／または菌糸を形成するものをいう。 As used herein, the term “mold” refers to filamentous fungi belonging to fungi and forms conidia and / or mycelium in the life cycle.
本明細書において使用する場合、用語「真菌類」とは、菌類であって、５界説における菌界から、細胞性粘菌類とラビリンツラ類を除く菌類の総称である。真菌類には、カビおよび酵母が含まれる。 As used herein, the term “fungi” is a general term for fungi and excluding cellular slime molds and labyrinths from the fungal kingdom in the 5th-century theory. Fungi include mold and yeast.
本発明方法の保存対象としての微生物は、好ましくは、真菌類および細菌類、より好ましくは真菌類、さらにより好ましくはカビである。本発明方法の保存対象としての微生物は、好ましくは、宿主細胞に感染するか、または共生者と共生する微生物である。共生としては、相利共生、片利共生、および、寄生が挙げられる。本発明の微生物が共生者と共生する場合、共生体を生成する。 The microorganisms to be stored in the method of the present invention are preferably fungi and bacteria, more preferably fungi, and still more preferably mold. The microorganism to be stored in the method of the present invention is preferably a microorganism that infects a host cell or symbiotic with a symbiotic person. Symbiosis includes mutualism, symbiosis, and parasitism. When the microorganism of the present invention coexists with a symbiotic person, a symbiotic organism is generated.
本発明の微生物としては、例えば、以下が挙げられるが、これに限定されない：
Ｅｒｙｓｉｐｈｅ ｐｏｌｙｇｏｎｉ、Ｅｒｙｓｉｐｈｅ ｐｉｓｉ、Ｅｒｙｓｉｐｈｅ ｐａｅｏｎｉａｅ、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅｒｕｍ、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅｒｕｍ ｖａｒ．ｃｉｃｈｏｒａｃｅｒｕｍ、Ｓｐｈａｅｒｏｔｈｅｃａ ｐａｎｎｏｓａ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｌｉｇｉｎｅａ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｉｌｉｐｅｎｄｕｌａｅ、Ｓｐｈａｅｒｏｔｈｅｃａ ｂａｌｓａｍｉｎａｅ、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｓｃａ、Ｕｎｃｉｎｕｌａ ｓｉｍｕｌａｎｓ、Ｏｉｄｉｕｍ ｂｅｇｏｎｉａｅ ｖａｒ．ｍａｃｒｏｓｐｏｒｕｍ、Ｏｉｄｉｕｍ ｓｐ．、Ｓｐｈａｅｒｏｔｈｅｃａ ｈｕｍｕｌｉ、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｅｒｙｓｉｐｈｅ：ａｃｔｉｎｏｓｔｅｍｍａｔｉｓ Ｂｒａｕｎ［ゴキヅル・ミヤマニガウリうどんこ病］、ａｑｕｉｌｅｇｉａｅ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ａｑｕｉｌｅｇｉａｅ［オダマキうどんこ病］、ａｑｕｉｌｅｇｉａｅ ｖａｒ．ｒａｎｕｎｃｕｌｉ（Ｇｒｅｖｉｌｌｅ）Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［デルヒニュウム・ラナンキュウラス・トリカブト類・アキカラマツうどんこ病］、ｂｒａｕｎｉｉ Ｎｏｍｕｒａ［アサマヒゴタイうどんこ病］、ｂｅｔａｅ（Ｖａｎｈａ）Ｗｅｌｔｚｉｅｎ［アリタソウ・ケアリタソウうどんこ病］、ｃａｕｃａｓｉｃａ Ｓｉｍｏｎｉａｎ ｖａｒ．ｃｏｒｃｈｏｒｉ Ｔａｎｄａ［モロヘイヤうどんこ病］、ｃｅｌｏｓｉａｅ Ｔａｎｄａ［ノゲイトウうどんこ病］、ｃｈｌｏｒａｎｔｈｉ（Ｇｏｌｏｖｉｎ ｅｔ Ｂｕｎｋｉｎａ）Ｂｒａｕｎ［フタリシズカうどんこ病］、ｄｉｅｒｖｉｌｌａｅ Ｍｉｙａｂｅ ｅｘ Ｂｒａｕｎ［ハコネウツギ類うどんこ病］、ｇｌｙｃｉｎｅｓ Ｔａｉ ｅｍｅｎｄ Ｚｈｅｎｇ ｖａｒ．ｇｌｙｃｉｎｅｓ［ヤブマメ・ナツフジ・ヤブハギ類うどんこ病］、ｇｌｙｃｉｎｅｓ ｖａｒ ｌｅｓｐｅｄｅｚａｅ（Ｚｈｅｎｇ ｅｔ Ｂｒａｕｎ）Ｂｒａｕｎ ｅｔ Ｚｈｅｎｇ［ケハギ・メドハギうどんこ病］、ｇｒａｃｉｌｉｓ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ ｖａｒ．ｇｒａｃｉｌｉｓ［アラカシ・シラカシ・ウラジロカシうどんこ病］、ｇｒａｃｉｌｉｓ ｖａｒ．ｌｏｎｇｉｓｓｉｍａ Ｎｏｍｕｒａ［アカガシ・ツクバネガシうどんこ病］、ｈｅｒａｃｌｅｉ ｄｅ Ｃａｎｄｏｌｌｅ［ニンジン・パセリうどんこ病］、ｈｏｍｍａｅ Ｂｒａｕｎ［ナギナタコウジュ・ナミキソウ類うどんこ病］、ｈｕａｙｉｎｅｎｓｉｓ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［ヤマハッカうどんこ病］、ｌｙｔｈｒｉ Ｊｕｎｅｌｌ［ミソハギ・エゾミソハギうどんこ病］、ｍａｃｌｅａｙａｅ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［タケニグサうどんこ病］、ｍａｌｌｏｔｉ Ｃｈｅｎ ｅｔ Ｇａｏ［アカメガシワうどんこ病］、ｍａｙｏｒｉｉ Ｂｌｕｍｅｒ ｖａｒ．ｊａｐｏｎｉｃａ Ｂｒａｕｎ ｅｔ Ｎｏｍｕｒａ［アザミ類うどんこ病］、ｍａｙｏｒｉｉ Ｂｌｕｍｅｒ ｖａｒ．ｍａｙｏｒｉｉ［ノアザミうどんこ病］、ｏｔａｎｉｉ Ｎｏｍｕｒａ［ムカゴイラクサうどんこ病］、ｐａｅｏｎｉａｅ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ［シャクヤクうどんこ病］、ｐｉｌｅａｅ Ｂｒａｕｎ［ミズ類うどんこ病］、ｐｉｓｉ ｄｅ Ｃａｎｄｏｌｌｅ［エンドウ・キバナオウギ・クララ・ルピナスうどんこ病］、ｐｌｅｃｔｒａｎｔｈｉ Ｓｈｉｎ ｅｔ Ｌａ［オオヤマハッカうどんこ病］、ｐｏｌｙｇｏｎｉ ｄｅ Ｃａｎｄｏｌｌｅ［ソバ・タデ類うどんこ病］、ｐｕｓｉｌｌａ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［マルバネコノメうどんこ病］、ｒｏｄｇｅｒｉｓｉａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ［ヤグルマソウうどんこ病］、ｓｔｅｐｈａｎｉａｅ Ｔａｎｄａ［タマザキツヅラフジうどんこ病］ ｔａｋａｍａｔｓｕｉ Ｎｏｍｕｒａ［ハスうどんこ病］、ｕｌｍａｒｉａｅ Ｄｅｓｍａｚｉｅｒｅｓ［シモツケソウうどんこ病］、ｖｉｃｉａｅ−ｕｎｉｊｕｇａｅ（Ｈｏｍｍａ）Ｂｒａｕｎ［ナンテンハギうどんこ病］、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｍｉｃｒｏｓｐｈａｅｒａ：ａｂｅｌｉｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アベマキうどんこ病］、ａｋｅｂｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アケビうどんこ病］、ａｌｐｈｉｔｏｉｄｅｓ（Ｇｒｉｆｆｏｎ ｅｔ Ｍａｕｂｌａｎｃ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏｉｄｉｕｍ ｑｕｅｒｃｉｎｕｍ Ｔｈｕｍｅｎ ｖａｒ．ｇｅｍｍｉｐａｒｕｍ Ｆｅｒｒａｒｉｓ、クヌギ・ミズナラ・スダジイうどんこ病］、ｂｅｒｂｅｒｉｄｉｃｏｌａ Ｔａｉ［ホソバヒイラギナンテン・メギうどんこ病］、ａｓｔｒａｇａｌｉ ｄｅ Ｃａｎｄｏｌｌｅ［モメンヅルうどんこ病］、ｂａｕｍｅｌｅｒｉ（Ｍａｇｎｕｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クサフジ・ツルフジバカマ・ヨツバハギうどんこ病］、ｂｅｒｂｅｒｉｄｉｃｏｌａ（Ｆ．Ｌ．Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ホソバヒイラギナンテンうどんこ病］、ｂｅｒｂｅｒｉｄｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ａｓｉａｔｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒロハヘビノボラズ・メギうどんこ病］、ｂｅｒｃｈｅｍｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クマヤナギ類うどんこ病］、ｂｌａｓｔｉ（Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオモジ・クロモジうどんこ病］、ｃａｌｏｃｌａｄｏｐｈｏｒａ（Ａｔｋｉｎｓｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アメリカシロナラうどんこ病］、ｃｌｅｔｈｒａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［リョウブうどんこ病］、ｃｏｒｉａｒｉａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ドクウツギうどんこ病］、ｃｏｒｙｌｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハシバミ・ツノハシバミうどんこ病］、ｄｅｕｔｚｉａｅ（Ｂｕｎｋｉｎａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ウツギうどんこ病］、ｅｒｌａｎｇｓｈａｎｅｎｓｉｓ（Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ミヤマウグイスカグラうどんこ病］、ｆｒｉｅｓｔｉｉ Ｌｅｖｅｉｌｌｅ ｖａｒ．ｄａｈｕｒｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クロツバラ・クロウメモドキうどんこ病］、ｈｅｌｗｉｎｇｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハナイカダうどんこ病］、ｈｏｍｍａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハシバミ類うどんこ病］、ｉｔｏａｎａ（Ｎｏｍｕｒａ， Ｔａｎｄａ ｅｔ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オケラうどんこ病］、ｉｚｕｅｎｓｉｓ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツツジ類うどんこ病］、ｊｕｇｌａｎｄｉｓ（Ｇｏｌｏｖｉｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クルミ・サワグルミうどんこ病］、ｋａｔｕｍｏｔｏｉ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバイボタうどんこ病］、ｌｉｇｕｓｔｒｉ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イボタノキうどんこ病］、ｌｏｎｉｃｅｒａｅ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ｌｏｎｉｃｅｒａｅ［スイカズラうどんこ病］、ｍａｇｎｉｆｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［モクレン・コブシうどんこ病］、ｍａｇｎｏｌｉａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ホオノキうどんこ病］、ｍａｙｕｍｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［マユミうどんこ病］、ｍｅｌｉｏｓｍａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アワブキうどんこ病］、ｍｅｎｉｓｐｅｒｍｉ Ｈｏｗｅ ｖａｒ．ｄａｈｕｒｉｃａ（Ｂｒａｕｎ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コウモリカズラうどんこ病］、ｍｅｎｉｓｐｅｒｍｉ Ｈｏｗｅ ｖａｒ．ｓｉｎｏｍｅｎｉｉ（Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツヅラフジうどんこ病］、ｍｉｕｒａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［キンギンボク・チシマヒョウタンボクうどんこ病］、ｍｉｙａｂｅａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［エゴノキうどんこ病］、ｍｕｌｔｉａｐｐｅｎｄｉｃｉｓ （Ｚｈａｏ ＆ Ｙｕ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒロハヘビノボラズうどんこ病］、ｎｏｍｕｒａｅ Ｂｒａｕｎ［サワフタギうどんこ病］、ｏｒｉｘａｅ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コクサギうどんこ病］、ｐｅｎｉｃｉｌｌａｔａ（Ｗａｌｌｒｏｔｈ）Ｌｉｎｋ［ケヤマハンノキ・ミヤマハンノキ・ヒメヤシャブシうどんこ病］、ｐｈｙｌｌａｎｔｈｉ（Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コバンノキうどんこ病］、ｐｉｃｒａｓｍａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニガキうどんこ病］、ｐｓｅｕｄｏｌｏｎｉｃｅｒａｅ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオツヅラフジうどんこ病］、ｐｕｌｃｈｒａ（Ｃｏｏｋ ｅｔ Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｐｕｌｃｈｒａ［ハナミズキ・ヤマボウシ、ミズキうどんこ病］、ｒｏｂｉｎｉｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハリエンジュうどんこ病］、ｒｕｓｓｅｌｉｉ（Ｃｌｉｎｔｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カタバミ類うどんこ病］、ｓｃｈｉｚａｎｄｒａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［マツブサうどんこ病］、ｓｅｃｕｒｉｎｅｇａｅ（Ｔａｉ ＆ Ｗｅｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒトツバハギうどんこ病］、ｓｈｉｎａｎｏｅｎｓｉｓ（Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カンボクうどんこ病］、ｖｉｂｕｒｎｉ Ｄｕｂｙ［ガマズミ類うどんこ病］、ｓｔａｐｈｙｌｅａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ミツバウツギうどんこ病］、ｓｕｂｔｒｉｃｈｏｔｏｍａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハリエンジュうどんこ病］、ｓｙｒｉｎｇａｅ−ｊａｐｏｎｉｃａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ライラック類うどんこ病］、ｔｉｌｉａｅ（Ｅｌｉａｄｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバボタイジュうどんこ病］、ｔｒｉｆｏｌｉｉ Ｇｒｅｖｉｌｌｅ ｖａｒ．ｔｒｉｆｏｌｉｉ［アカクローバうどんこ病］、ｖａｎｂｒｕｎｔｉａｎａ Ｇｅｒａｒｄ ｖａｒ．ｓａｍｂｕｃｉ−ｒａｃｅｍｏｓａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニワトコうどんこ病］、ｗａｌｌｒｏｔｈｉｉ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［スノキ類うどんこ病］、ｗｅｉｇｅｌａｅ−ｄｅｃｏｒａｅ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニシキウツギうどんこ病］、ｙａｍａｄａｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ケンポナシうどんこ病］、Ｍｉｃｒｏｓｐｈａｅｒａ ｌｏｎｉｃｅｒａｅ−ｒａｍｏｓｉｓｓｉｍａｅ Ｔａｎｄａ［コウグイスカグラうどんこ病］、ｑｕｅｒｃｉ Ｓａｗａｄａ［ナラ類・ウバメガシうどんこ病］、ｓｃｈｉｚａｎｄｒａｅ Ｓａｗａｄａ ｆ．ｂｒｅｖｉｓｅｔａ Ｔａｎｄａ ＆ Ｎｏｍｕｒａ［チョウセンゴミシうどんこ病］、ｓｉｎｅｎｓｉｓ Ｙｕ［クリうどんこ病］、Ｅｒｙｓｉｐｈｅ ｓｅｃｔｉｏｎ Ｕｎｃｉｎｕｌａ：ａｃｔｉｎｉｄｉａｅ（Ｍｉｙａｂｅ ｅｘ Ｊａｃｚｅｗｓｋｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ａｃｔｉｎｉｄｉａｅ［マタタビ・サルナシうどんこ病］、ａｃｔｉｎｉｄｉａｅ ｖａｒ．ａｒｇｕｔａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サルナシ・マタタビうどんこ病］、ａｄｕｎｃａ（Ｗａｌｌｒｏｔｈ）Ｆｒｉｅｓ ｖａｒ．ａｄｕｎｃａ［バッコヤナギうどんこ病］、ａｄｕｎｃａ ｖａｒ．ｍａｎｄｓｈｕｒｉｃａ（Ｍｉｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヤナギ類うどんこ病］、ａｍａｎｏｉ（Ｎｏｍｕｒａ）Ｂｒａ
ｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒメヤシャブシうどんこ病］、ａｕｓｔｒａｌｉａｎａ（ＭｃＡｌｐｉｎｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サルスベリうどんこ病］、ｂｅｔｕｌｉｎａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カンバ類うどんこ病］、ｂｉｆｕｒｃａｔａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コナラうどんこ病］、ｂｕｃｋｌｅｙａｅ（Ｎｏｍｕｒａ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツクバネうどんこ病］、ｃａｒｐｉｎｉｃｏｌａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シデ類うどんこ病］、ｃｅｄｒｅｌａｅ（Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チャンチンうどんこ病］、ｃｌａｎｄｅｓｔｉｎａ Ｂｉｖｏｎａ−Ｂｅｒｎａｒｄｉ［ニレ類うどんこ病］、ｃｌｉｎｔｏｎｉｏｐｓｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｃｕｒｖｉｓｐｏｒａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イヌブナうどんこ病］、ｅｈｒｅｔｉａｅ（Ｋｅｉｓｓｌ．）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔａｉｗａｎｅｎｓｉｓ（Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チシャノキうどんこ病］、ｆｒａｘｉｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｈｙｄｒａｎｇｅａｅ（Ｃｈｅｎ ＆ Ｇａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノリウツギうどんこ病］、ｋｕｓａｎｏｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｋｕｓａｎｏｉ［エノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ａｐｈａｎａｎｔｈｅｓ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムクノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ｚｅｌｋｏｗａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ケヤキうどんこ病］、ｌａｔａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニシキウツギうどんこ病］、ｌｊｕｂａｒｓｋｉｉ ｖａｒ．ａｄｕｎｃｏｉｄｅｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオイタヤメイゲツうどんこ病］、ｌｊｕｂａｒｓｋｉｉ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｌｊｕｂａｒｓｋｉｉ［イロハモミジ・ヤマモミジうどんこ病］、ｍａｔｓｕｎａｍｉａｎａ（Ｎｏｍｕｒａ， Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヤマハゼうどんこ病］、ｍｉｙａｂｅｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハンノキ類うどんこ病］、ｍｏｒｉ（Ｍｉｙａｋｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ｎｅｃａｔｏｒ［ブドウうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ａｍｐｅｌｏｐｓｉｄｉｓ（Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノブドウうどんこ病］、ｎｉｓｈｉｄａｎａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｏｌｅｏｓａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｏｌｅｏｓａ［シナノキうどんこ病］、ｏｌｅｏｓａ ｖａｒ．ｚｈｅｎｇｉｉ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバボダイジュうどんこ病］、ｐｉｃｒａｓｍａｅ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニガキうどんこ病］、ｐｓｅｕｄｏｃａｒｐｉｎｉｃｏｌａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サワシバうどんこ病］、ｓａｌｉｃｉ−ｇｒａｃｉｌｉｓｔｙｌａｅ Ｈｏｍｍａ［カワヤナギ・ネコヤナギうどんこ病］、ｓａｌｍｏｎｉｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｓｃｈｉｚｏｐｈｒａｇｍａｔｉｓ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イワガラミうどんこ病］、ｓｅｎｇｏｋｕｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツルウメモドキうどんこ病］、ｓｅｐｔａｔａ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ＊［コナラ・カシワうどんこ病］、ｓｉｍｕｌａｎｓ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｓｉｍｕｌａｎｓ［ノイバラうどんこ病］、ｓｉｍｕｌａｎｓ ｖａｒ．ｔａｎｄａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノイバラうどんこ病］、ｔｏｇａｓｈｉａｎａ ｖａｒ．ｒｉｇｉｄａ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバアサガラうどんこ病］、ｔｏｇａｓｈｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔｏｇａｓｈｉａｎａ［ハクウンボクうどんこ病］、ｖｅｒｎｉｃｉｆｅｒａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ウルシ・ヤマハゼ・ヌルデ・ケムリノキうどんこ病］、ｖｉｂｕｒｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガマズミ類うどんこ病］、Ｕｎｃｉｎｕｌａ ａｓｔｅｒｉｓ Ｓａｗａｄａ［ノコンギクうどんこ病］、ｃａｒｐｉｎｉ−ｃｏｒｄａｔａｅ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［サワシバうどんこ病］、ｍｉｙａｂｅｉ ｖａｒ．ｈｅｒｍａｐｈｒｏｄｉｔｉｃａ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［ヒメヤシャブシうどんこ病］、Ｇｏｌｏｖｉｎｏｍｙｃｅｓ：ａｄｅｎｏｐｈｏｒａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［ツリガネニンジン・ソバナうどんこ病］、ａｒａｂｉｄｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［エゾハタザオうどんこ病］、ａｒｔｅｍｉｓｉａｅ（Ｇｒｅｖｉｌｌｅ）Ｇｅｌｊｕｔａ［ヨモギ・オオヨモギ・オトコヨモギうどんこ病］、ｂｉｏｃｅｌｌａｔｕｓ（Ｅｈｒｅｎｂｅｒｇ）Ｇｅｌｊｕｔａ ｖａｒ．ｍｏｎａｒｄａｅ〔モナルダうどんこ病〕、ｂｒａｕｎｅｏｐｕｎｃｔａｔａ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ミゾホオズキうどんこ病］、ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ［ノコギリソウ類・アザミ類・ヒヨドリバナ類・ハンゴンソウ・ノゲシ・ホオズキなどナス科・キク科植物の多数のうどんこ病］、ｌａｐｏｒｔｅａｅ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ムカゴイラクサうどんこ病］、ｓｏｒｄｉｄｕｓ（Ｊｕｎｅｌｌ）Ｇｅｌｊｕｔａ［オオバコうどんこ病］、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅａｒｕｍ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ｌａｔｉｓｐｏｒａ Ｂｒａｕｎ［ブタクサうどんこ病］、Ｎｅｏｅｒｙｓｉｐｈｅ：ｃｕｍｍｉｎｓｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ［モミジガサ・メタカラコウ・テバコモミジガサうどんこ病］、ｇａｌｅｏｐｓｉｄｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｂｒａｕｎ［オドリコソウ・ジャコウソウ・イヌゴマ・ヤマハッカ・ヒキオコシうどんこ病］、ｇｅｒａｎｉｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ［ゲンノショウコ・チシマフウロ・イチゲフウロ・エゾフウロ・ハクサンフウロうどんこ病］、Ｐｈｙｌｌａｃｔｉｎｉａ：ａｉｌａｎｔｈｉ（Ｇｏｌｏｖｉｎ ｅｔ Ｂｕｎｋｉｎａ）Ｙｕ［ニワウルシうどんこ病］、ａｌｎｉ Ｙｕ ＆ Ｈａｎ［ハンノキ類うどんこ病］、ａｍｐｅｌｏｐｓｉｄｉｓ Ｙｕ ＆ Ｌａｉ［サンカクヅルうどんこ病］、ｂｒｏｕｓｓｏｎｅｔｉａｅ−ｋａｅｍｐｆｅｒｉ Ｓａｗａｄａ［コウゾ裏うどんこ病］、ｆｒａｘｉｎｉ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｆｕｓｓ［トネリコ類裏うどんこ病・マサキうどんこ病］、ｇｕｔｔａｔａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ［ハシバミ類・マンサク・コブシ・マタタビ・裏うどんこ病・カンバ類・ハンノキ類・サワシバ・イヌシデ・フサザクラ・ニガキ・オオバアサガラ・ネジキ・ジャケツイバラうどんこ病］、ｊｕｇｌａｎｄｉｓ Ｔａｏ ＆ Ｑｉｎ［オニクルミ・サワグルミうどんこ病・シナサワグルミ裏うどんこ病］、ｋａｋｉｃｏｌａ Ｓａｗａｄａ［カキうどんこ病］、ｍａｇｎｏｌｉａｅ Ｙｕ ＆ Ｌａｉ［コブシ類裏うどんこ病］、ｍａｌｉ（Ｄｕｂｙ）Ｂｒａｕｎ［ナシ・セイヨウナシうどんこ病］、ｍｏｒｉｃｏｌａ（Ｈｅｎｎｉｎｇｓ）Ｈｏｍｍａ［クワ裏うどんこ病］、ｐｔｅｒｏｓｔｙｒａｃｉｓ Ｙｕ ＆ Ｌａｉ［オオバアサガラ裏うどんこ病］、ｒｏｂｏｒｉｓ（Ｇａｃｈｅｔ）Ｂｌｕｍｅｒ［クリ・アラカシ裏うどんこ病］、ｓａｌｍｏｎｉｉ Ｂｌｕｍｅｒ［キリうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｐｏｄｏｓｐｈａｅｒａ：ｃｅｒｃｉｄｉｐｈｙｌｌｉ Ｔａｎｄａ ＆ Ｎｏｍｕｒａ［カツラうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ａｕｃｕｐａｒｉａｅ（Ｅｒｉｋｓｓｏｎ）Ｂｒａｕｎ［ナナカマドうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ｃｌａｄｅｓｔｉｎａ［シモツケ類うどんこ病］、ｃｕｒｖｉｓｐｏｒａ Ｎｏｍｕｒａ［ナナカマドうどんこ病］、ｌｅｕｃｏｔｒｉｃｈａ（Ｅｌｌｉｓ ＆ Ｅｖｅｒｈａｒｔ）Ｓａｌｍｏｎ ｖａｒ．ｌｅｕｃｏｔｒｉｃｈａ［不完全世代：Ｏｉｄｉｕｍ ｆａｒｉｎｏｓｕｍ Ｃｏｏｋｅ、リンゴ・ズミうどんこ病］、ｌｏｎｇｉｓｅｔａ Ｓａｗａｄａ［ウワミズザクラ・ザイフリボクうどんこ病］、ｍｙｒｔｉｌｌｉｎａ（Ｓｃｈｂ．Ｆｒ）Ｋｕｎｚｅ ｖａｒ．ｍｙｒｔｉｌｌｉｎａ［クロウスゴうどんこ病］、ｓｐｉｒａｌｉｓ Ｍｉｙａｂｅ［ハルニレうどんこ病］、ｔｒｉｄａｃｔｙｌａ（Ｗａｌｌｒｏｔｈ）ｄｅ Ｂａｒｙ ｖａｒ．ｔｒｉｄａｃｔｙｌａ［不完全世代：Ｏｉｄｉｕｍ ｐａｓｓｅｒｉｎｉｉ Ｂｅｒｔｏｌｏｎｉ、モモ・スモモ・アンズ・ウメ・ユスラウメ・ニワウメ・サクラうどんこ病］、ｖｉｂｕｒｎｉ Ｂｒａｕｎ［ガマズミう類どんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｐ．［カマツカうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｓｐｈａｅｒｏｔｈｅｃａ：ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ａｐｈａｎｉｓ［不完全世代：Ｏｉｄｉｕｍ ｒｕｂｏｒｕｍ Ｒａｂｅｎｈｏｒｓｔ、イチゴうどんこ病］、ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ｖａｒ．ｈｙａｌｉｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［キジムシロうどんこ病］、ａｓｔｉｌｂｉｃｏｌａ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカショウマ・トリアシショウマうどんこ病］、ｂａｌｓａｍｉｎａｅ（Ｋａｒｉ ｅｘ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏｉｄｉｕｍ ｂａｌｓａｍｉｎａｅ Ｒａｊｄｅｒｋａｒ、ホウセンカ・キツリフネ・ツリフネソウうどんこ病］、ｃａｌｌｉｃａｒｐａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムラサキシキブうどんこ病］、ｃａｒｄａｍｉｎｅｓ Ｎｏｍｕｒａ［タネツケバナうどんこ病］、ｃａｒｉｃａｅ−ｐａｐａｙａｅ（Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｃａｒｉｃａｅ Ｎｏａｃｋ、パパイヤうどんこ病］、ｃｕｃｕｒｂｉｔａｅ（Ｊａｃｚｅｗｓｋｉｉ）Ｚｈａｏ［キュウリ・カボチャ・セイヨウカボチャ・ヒョウタンのうどんこ病］、ｄｉｃｌｉｐｔｅｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハグロソウうどんこ病］、ｅｐｉｌｏｂｉｉ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカバナ類うどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｅｌｉｏｓｃｏｐｉａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トウダイグサうどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｉｒｔａｅ（Ｂｒａｕｎ ＆ Ｓｏｍａｎｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［エノキグサうどんこ病］、ｆｅｒｒｕｇｉｎｅａ ｖａｒ．ａｌｂｉｆｌｏｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シロバナトウウチソウうどんこ病］、ｆｅｒｒｕｇｉｎｅａ（Ｓｃｈｌｅｃｈｔｅｎｄａｈｌ ｅｘ Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｆｅｒｒｕｇｉｎｅａ［ワレモコウうどんこ病］、ｆｕｇａｘ（Ｐｅｎｚｉｇ ＆ Ｓａｃｃａｒｄ）Ｂｒ
ａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ゲンノショウコうどんこ病］、ｆｕｓｃａ（Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［不完全世代：Ｏ．ｃｉｔｒｕｌｌｉ Ｙｅｎ ＆ Ｗａｎｇ、キュウリ・カボチャ・ユウガオ・トウガン・ゴボウ・フキ・コスモス・ヒマワリ・キクイモ・ペチュニア・オオキンケイギク・シオン・ダリア・リュウゼツサイ・ルドベキアうどんこ病］、ｈｉｂｉｓｃｉｃｏｌａ Ｚｈａｏ［フヨウうどんこ病］、ｉｎｔｅｒｍｅｄｉａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クサギうどんこ病］、ｍｏｒｓ−ｕｖａｅ（Ｓｃｈｗｅｉｎｉｔｚ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［スグリ類うどんこ病］、ｐａｎｎｏｓａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）ｄｅ Ｂａｒｙ［不完全世代：Ｏ．ｌｅｕｃｏｃｏｎｉｕｍ Ｄｅｓｍａｚｉｅｒｅｓ， バラ・ハマナスうどんこ病］、ｐｈａｓｅｏｌｉ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アズキ・ヤブツルアズキうどんこ病］、ｐｓｅｕｄｏｆｕｓｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワクサうどんこ病］、ｓｉｂｉｒｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クガイソウ・コシオガマうどんこ病］、ｓｐａｒｓａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガガイモ・オオカモメズルうどんこ病］、ｓｐｉｒａｅａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｂｏｔｒｙｏｉｄｅｓ（Ｃｏｒｄａ）Ｃｅｓａｔｉ、シジミバナ・ユキヤナギ・オニシモツケうどんこ病］、ｓｔｅｐｈａｎａｎｄｒａｅ（Ｊａｃｚｅｗｓｋｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コゴメウツギうどんこ病］、ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［ウリ類・ヒマワリ・ヒャクニチソウ・フキ・ゴボウ・アメリカセンダングサ・キンセンカ・ベニバナ・アキノノゲシ・ヤマニガナ・ガーベラ・フヨウ・ナス・ヤブガラシ・エノキグサなど多数のうどんこ病］、Ｃｙｓｔｏｔｈｅｃａ：ｗｒｉｇｈｔｉｉ Ｂｅｒｋｅｌｅｙ ＆ Ｃｕｒｔｉｓ［カシ類紫かび病］、ｌａｎｅｓｔｒｉｓ（Ｈａｒｋｎｅｓｓ）Ｍｉｙａｂｅ［ナラ類紫かび病］、Ｔｙｐｈｕｌｏｃｈａｅｔａ：ｊａｐｏｎｉｃａ Ｉｔｏ ＆ Ｈａｒａ［ナラガシワ・ミズナラ・コナラ・トネリコうどんこ病］、Ｓａｗａｄａｅａ：ｂｉｃｏｒｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｍｉｙａｂｅ［カエデ類うどんこ病］、ｐｏｌｙｆｉｄａ（Ｗｅｉ）Ｚｈｅｎｇ ＆ Ｃｈｅｎ ｖａｒ．ｊａｐｏｎｉｃａ Ｂｒａｕｎ ＆ Ｔａｎｄａ［モミジ類・カエデ類うどんこ病］、ｔｕｌａｓｎｅｉ（Ｆｕｃｋｅｌ）Ｈｏｍｍａ［カエデ類うどんこ病］、ｚｈｅｎｇｉｉ Ｎｏｍｕｒａ［エンコウカエデうどんこ病］、Ｂｌｕｍｅｒｉａ：ｇｒａｍｉｎｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｓｐｅｅｒ［ムギ類うどんこ病］、Ｐｌｅｏｃｈａｅｔａ：ｓｈｉｒａｉａｎａ（Ｈｅｎｎｉｎｇｓ）Ｋｉｍｂｒｏｕｇｈ ＆ Ｋｏｒｆ［エノキ・ムクノキうどんこ病］、などのうどんこ病の病原菌；
Ｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｓｅｕｄｏｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｌａｓｍｏｐａｒａ ｓｐｐ．、Ｂｒｅｍｉａ ｓｐｐ．、および、Ｂｒｅｍｉｅｌｌａ ｓｐｐ．などのべと病の病原菌；
Ｃｒｏｎａｒｔｉｕｍ ｓｐｐ．、Ｍｅｌａｍｐｓｏｒａ ｓｐｐ．、Ｐｈａｋｏｐｓｏｒａ ｓｐｐ．、Ｕｒｅｄｉｎｏｐｓｉｓ ｓｐｐ．、Ｂｌａｓｔｏｓｐｏｒａ ｓｐｐ．、Ｇｙｍｎｏｓｐｏｒａｎｎｇｉｕｍ ｓｐｐ．、Ｐｕｃｃｉｎｉａ ｓｐｐ．、Ｕｒｏｍｙｃｅｓ ｓｐｐ．、Ａｅｃｉｄｉｕｍ ｓｐｐ．、Ｃａｅｍａ ｓｐｐ．、Ｐｅｒｉｄｅｒｍｉｕｍ ｓｐｐ．、Ｕｒｅｄｏ ｓｐｐ．、Ｐｈｒａｇｍｉｄｉｕｍ ｓｐｐ．、Ｓｔｅｒｅｏｓｔｒａｔｕｍ ｓｐｐ．などのさび病の病原菌；ならびに、
Ｔｒｉｃｈｏｌｏｍａ ｍａｔｓｕｔａｋｅ（Ｓ．Ｉｔｏ ｅｔ Ｉｍａｉ）Ｓｉｎｇ．、Ｔｕｂｅｒ ｓｐｐ．、Ｓｕｉｌｌｕｓ ｂｏｖｉｎｅｓ、および、Ｒｈｉｚｏｐｏｇｏｎ ｒｕｂｅｓｃｅｎｓ、および、ＶＡ菌根などの菌根菌。
Examples of the microorganism of the present invention include, but are not limited to, the following:
Erysiphe polygoni, Erysiphe pisii, Erysiphe paeoniae, Erysiphe cichoracerum, Erysiphe cichocererum var. cichoracerum, sphaerotheca pannosa, sphaerotheca furiginea, sphaerotheca filipendulae, sphaerotheca balsamine bumamine, sphaerotheca fuscaulc. macrosporum, Oidium sp. , Sphaerotheca humuli, Erysiphe section Erysiphe: actinostematis Braun [Gokiru Miyamanigauri powdery mildew], aquilegiae de Candole var. aquilegiae [Audamaki powdery mildew], aquilegiae var. ranunculi (Greville) Zheng et Chen [Derhinium ranunculus, aconite, Akikara pine powdery mildew], braunii Nomura [Asahi goitou powdery mildew], betae (Vanha) Weltzien vice . corchori Tanda [Morohaya powdery mildew], celosiae Tanda [Nogateto powdery mildew], chloranthi (Golovin et Bunkina) Braun [Futarisuka powdery gourd disease] glycines [Yabamame, Natsufuji, Yabuhagi powdery mildew], glycines var lespedezae (Zheng et Braun) Braun et Zheng [Kehagigi Medhagi powdery mildew], gracilis Zhengvet gracilis [Aracasi, Shirakasi, Vulgaris mildew], gracilis var. longisima Nomura [red-eyed tsukubagasashi powdery mildew], heraclei de Candole [carrot, parsley powdery mildew], hommae Braun [naginata-jutsu-namiki-shon-powder-disease], huainensis Misohagi, Ezomisohagi powdery mildew], macleayae Zheng et Chen [takenigusa powdery mildew], malloti Chen et Gao [Akamegasiwa powdery mildew], mayorii Blumer var. japonica Braun et Nomura [thistle powdery mildew], mayorii Blumer var. mayorii [Noasami powdery mildew], otanii Nomura [Mungougoura powdery mildew], paeoniae Zheng et Chen [Peonies powdery mildew], pileae Braun [Mizudo powdery mildew], pile basil Powdery mildew], plectranthi Shin et La [Prunus serrata powdery mildew], polygoni de Candole [soba-tade powdery mildew], psilla Tanda et Nomura [Mulban conodium powdery mildew], rodgerisula Disease], stefaniae Tanda [Tamazakitsu rafuji powdery mildew] takamatsusui Nomur [Lotus powdery mildew], ulmariae Desmazeres [Pleurotus powdery mildew], Biciae-unijugae (Homma) Braun [Nantenhagi powdery mildew] & Takamatsu [Akebi powdery mildew], alphaitoides (Griffon et Maublanc) Braun & Takamatsu [Incomplete generation: Oidium quarcinum Thumen var. gemmiparam Ferraris, Kunugi Mizunara Sudazii powdery mildew], berberidicola Tai [Hobas holly guinea tenge powdery mildew], astragali de Candole Disease], berberidicola (F.L. Tai) Braun & Takamatsu [mushroom powdery mildew], berberidis (de Candole) Level var. asiatica (Braun) Braun & Takamasu [Hiroha snake noboraz barberry powdery mildew], berchemiae (Sawada) Braun & Takamatu [Black-bellied powdery mildew], blasti (Tai) Braun ) Braun & Takamatsu [American white powdery mildew], clethrae (Braun) Braun & Takamatsu [Rybu powdery mildew], coriarie (Nomura) Braun & Takamatsu [Dokutsugi] Disease], de tziae (Bunkina) Braun & Takamatsu [Utsugi powdery mildew], erlangshanensis (Yu) Braun & Takamatsu [Miyama nightingale Kagura powdery mildew], friestii Leveille var. dahurica (Braun) Braun & Takamatsu [black rose rose buckthorn powdery mildew], helwiniae (Sawada) Braun & Takamatsu [Hanaika powdery mildew], hommae (Braun) et Braun) Braun & Takamatsu [Okella powdery mildew], izuensis (Nomura) Braun & Takamatsu [Azalea powdery mildew], juglandis (Golovin) Braun & Takamasu disease Takamatsu [Popular powdery mildew] , Ligustri (Homma) Braun & Takamatsu [privet powdery mildew], lonicerae de Candolle var. lonicerae [honeysuckle powdery mildew], magnifica (Braun) Braun & Takamatsu [mokuren kobushi powdery mildew], magnoliae (Sawada) Braun & Takamasu mum ] Meliosmae (Tanda & Nomura) Braun & Takamatsu [Awabuki powdery mildew], menispermi Howe var. dahurica (Braun & Nomura) Braun & Takamatsu [Bats powdery mildew], menispermi Howe var. Sinomenii (Yu) Braun & Takamasu [Tsurafuji powdery mildew]; Braun & Takamatsu [Hiroha snake noboraz powdery mildew], nomurae Braun [Sawafutagi powdery mildew], orixae (Braun & Tanda) Braun & Takamatu ], Phyllant hi (Tanda & Braun) Braun & Takamatsu [Pinpodium powdery mildew], picrasmae (Sawada) Braun & Takamatsu [Nigaki powdery mildew], pseudorucera (Salmon) Braun & Braun Braun & Takamatsu var. pulchra (dogwood, wild bovine, dogwood powdery mildew), robinicola Braun & Takamatsu (harienju powdery mildew), russerii (Clinton) Braun & Takamatsu (skinny powdery mildew) ], Securinegae (Tai & Wei) Braun & Takamatsu [Popular powdery mildew], Shinanoensis (Tanda) Braun & Takamatsu [Pumbo powdery], viburni Duby's disease Honeybee powdery mildew], s Ubrichochoma (Braun) Braun & Takamatsu [Harienju powdery mildew], syringae-japonicae (Braun) Braun & Takamatu [Lilac powdery mildew], tiliae (Eliade) Braun disease. trifolii [red clover powdery mildew], vanbruntiana Gerard var. Sambuci-racemosae (Braun) Braun & Takamatsu [Elderberry powdery mildew]; , Yamada (Salmon) Braun & Takamatsu [Kemponashi powdery mildew], Microsphaera lonicerae-Romasissimae Tanda [Kouisukagura powdery mildew, aqua Sawada] brevista Tanda & Nomura [Chrysanthemum powdery mildew], sinensis Yu [Chestnut powdery mildew], Erysiphe section Uncinula: actinidiae (Miyabe ex Jaczewski) Braun & Tamura. actinidiae [Matatabi / sarnashi powdery mildew], actinidiae var. argutae (Nomura) Braun & Takamatsu [Sarnashi Matatabi powdery mildew], adunaca (Wallroth) Fries var. adunca [Bacco willow powdery mildew], adunca var. mandhurica (Miura) Braun & Takamatsu [willow powdery mildew], amanoi (Nomura) Bra
un & Takamatsu [Prunus mildew], australiana (McAlpine) Braun & Takamatsu [Salum belly powdery], betulina Braun & Takamatsu [Umbrella] buckleyae (Nomura Tanda) Braun & Takamatsu [Tukubane powdery mildew], carpinicola (Hara) Braun & Takamatsu [sideworm powdery mildew], cedlelae (Tai) Braun & Takat. Powdery mildew], clin oniopsis (Zheng & Chen) Braun & Takamatsu [Sterculiaceae powdery mildew], curvispora (Hara) Braun & Takamatsu [Fagus japonica powdery mildew], ehretiae (Keissl.) Braun & Takamatsu var. taiwanensis (Tanda) Braun & Takamatu [Chishanoki powdery mildew], fraxinicola Braun & Takamasu [green potato powdery], hydrangeae (Chen & Gao) Braun & Takao. . Kusanoi [Enoki powdery mildew], Kusanoi var. aphananthes (Braun) Braun & Takamatsu [Mukunoki powdery mildew], kusanoi var. zelkowae (Hennings) Braun & Takamatsu [keyberry powdery mildew], lata (Nomura & Tanda) Braun & Takamatsu [Nishikitsugi powdery mildew], ljubarskii var. aduncoides (Zheng & Chen) Braun & Takamatsu [Ooitama Meigetsu powdery mildew], ljubarskii Braun & Takamatsu var. ljubarskii [Bloem & Bun], Matsunamiana (Nomura, Tanda & Braun), Braun & Takamatsu [Yamahose powdery mildew], Miyabei [Mulberry powdery mildew], necker Schweintz var. necator [grape powdery mildew], necator Schweintz var. ampelopsis (Peck) Braun & Takamatsu [no grape powdery mildew], nisidana (Homma) Braun & Takamatsu [Aogiri powdery mildew], oleosa (Zheng & Chen) Braun & Takam. oleosa [linden powdery mildew], oleosa var. zhengii (Braun) Braun & Takamatsu [Popular powdery mildew], picrasmae (Homma) Braun & Takamatsu [Nigaki powdery mildew], pseudocarpinicola (Nomura). River willow, cat willow powdery mildew], salmonii (Sydow) Braun & Takamatu [ash powdery mildew], schizophragmatis (Tanda & Nomura) Braun & Takamasu Key powdery mildew], septata (Salmon) Braun & Takamatsu * [Quercus Kashiwa powdery mildew], simulans (Salmon) Braun & Takamatsu var. simulans [Neubara powdery mildew], simulans var. tandae (Braun) Braun & Takamatsu [Neubara powdery mildew], togashiana var. rigida (Braun & Tanda) Braun & Takamatsu [Plump powdery mildew], togashiana (Braun) Braun & Takamatsu var. Togashiana [Hakuunboku powdery mildew], verniciferae (Hennings) Braun & Takamatsu [Urushi yamahaze, Nurde-Kemlino powdery mildew], viburnicola Braun & Tamamatsuu carpini-cordatae Tanda et Nomura [Sawasiba powdery mildew], Miyabei var. Hermaphroditica Tanda et Nomura [Hymeya Shabushi powdery mildew], Golovinomyces: adenophora (Zheng & Chen) Geljuta [Turiganinjin sobana powdery mildew]・ Two Artemisia, Paramecium powdery mildew], biocellatus (Ehrenberg) Geljuta var. monadae (monalda powdery mildew), brauneopuncta (Braun) Geljuta [Mizohozuki powdery mildew], cichoracerum (de Candole) Geljuta [achilleas, thistles, hydrangeas Powdery mildew], laporteae (Braun) Geljuta [Plumpy powdery mildew], sordidus (Junell) Geljuta [Plumpy powdery mildew], Erysiphe cichoracerum de Candole var. latispora Braun [Ragweed powdery mildew], Neoerysiphe: cumminsiana (Braun) Braun [Momigasa, Metakarako, Tebakomomigusa powdery mildew], galeopsidis (de Candole) geranii (Nomura) Braun [Gennoshouko, Chishimafuuro, Ichigefuuro, Ezofuuro, Hakusanfu powdery mildew], Phyllactinia: ailanthii (Golovin et Bunkina) Yu [Niwaurushi] & Lai [Sankakudon powdery mildew], br oussonetiae-kaempferi Sawada [Urban powdery mildew], fraxini (de Candole) Fuss [Glass powdery mildew / Masaki powdery mildew], gutata (Wallroth: Fries) Powdery mildew, birch, alder, sawnhorn, Insode, Fusazakura, Nigaki, Oobaasagara, Neki, Jukutsuibara powdery mildew, juglandis Tao & Qin [Oyster powdery mildew], magnoliae Yu & Lai [Kobushi back powdery mildew], mary (Duby) Braun [Nasis, pear udon] Disease], moricola (Hennings) Homma [back mulberry powdery mildew], pterostyracis Yu & Lai [Obaasagara back powdery mildew], roboris (Gachet) Blumer [chestnut arakashi back powdery mildew], salmoni ], Posphaera section Podophaera: cercidylphyli Tanda & Nomura [Katsura powdery mildew], cladestina (Wallroth: Fries) Level bar. aucupariae (Eriksson) Braun [Nanakamado powdery mildew], cladestina (Wallroth: Fries) Levelile var. cladestina [Pleurotus mushroom powdery mildew], curvispoora Nomura [rowan powdery mildew], leukotricha (Ellis & Everhart) Salmon var. leucotrica [incomplete generation: Oidium farinosum Cooke, apple / sumi powdery mildew], longiseta Sawada [waiwazuzakura zifriboku powdery mildew], myrtillina (Schb. Fr) Kunze var. myrtillina [Crousgo powdery mildew], spiralis Miyabe [harunile powdery mildew], tridactyla (Wallilloth) de Barry var. tridactyla [incomplete generation: Oidium passeriniii Bertononi, peach, peach, apricot, ume, eusloume, niwaume, cherry powdery mildew], viburni Braun [burdock powdery mildew], Podosphaera sp. [Kamatsuka powdery mildew], Podophaera section Sphaerotheca: aphanis (Wallillo) Braun & Takamatsu var. aphanis [incomplete generation: Oidium ruborum Rabenhorst, strawberry powdery mildew], aphanis (Wallilloth) Braun var. hyalina (Braun) Braun & Takamatsu [Kojimushi white powdery mildew], astilbicola (Zhao) Braun & Takamatsu [Red-skinned mumps], balsaminoe (Kari ex Braun) Spinach, tsutsurifune, tsutsufunesou powdery mildew], callicarpae (Tanda & Nomura) Braun & Takamatsu [purple powdery mildew], cardamines Nomura [rae] : O. caricae Noack, papaya powdery mildew], cucurbitae (Jaczewskii) Zhao [cucumber, pumpkin, pumpkin, leopard powdery mildew], diclipterae (Nomura) Braun & Tamagrospo [Red-bellied powdery mildew], euphorbiae-helioscopiae (Tanda & Nomura) Braun & Takamasu [euphorbia powdery mildew], euphorbiae-hirtae (Braun & Soma). albiflorae (Nomura) Braun & Takamatsu [powdery mildew powdery mildew], ferruginea (Schletchendahl ex Fries) Braun & Takamatsu var. ferruginea [Warming powdery mildew], fugax (Penzig & Saccard) Br
aun & Takamatsu [geno-shochu powdery mildew], fusca (Fries) Braun & Shishoff [incomplete generation: O. citrulli Yen & Wang, cucumber, pumpkin, yugao, tougan, burdock, burdock, cosmos, sunflower, chrysanthemum, petunia, pachytia, zion, dahlia, ryuzesai, rudbeckia powdery mildew, hibiscicola Braun) Braun & Takamatsu [Wagashi powdery mildew], mors-uvae (Schweinitz) Braun & Takamatsu [currant powdery mildew], pannosa (Wallroth: Fries) de Bary [Incomplete generation: O. leucoconium Desmazeres, Rose Hamanus powdery mildew], phaseoli (Zhao) Braun & Takamatsu [Azuki, Abutsuki Azuki powdery mildew], pseudofusca (Braun) braun & taku・ Koshio Gama powdery mildew], sparsa (Braun) Braun & Takamatsu [Gaigamo okamuzuru powdery mildew], spiraea (Sawada) Braun & Takamatsu [Incomplete generation: O. botryoides (Corda) Cesati, rainbow trout, snowy canopy, powdery mildews Numerous powdery mildews such as calendula, safflower, akinonogeshi, yamanana, gerbera, buoy, eggplant, sorghum, enokigusa], Cytotheca: wrighti Berkeley & Curtis [purple mold fungus], lanestris H Disease], Typhlochaeta: japonica to & Hara [Quercus Aliena, Japanese oak, Quercus, ash powdery mildew], Sawadaea: bicornis (Wallroth) Miyabe [maple such powdery mildew], polyfida (Wei) Zheng & Chen var. japonica Braun & Tanda [momiji, maple powdery mildew], tulasnei (Fuckel) Homma (maple powdery mildew), zhengii Nomura [enkookae powdery mildew], Blumeria: praminisum Pathogens of powdery mildew such as Pleochaeta: shiraiana (Hennings) Kimbrough & Korf [Enoki Mungoki powdery mildew];
Peronospora spp. , Pseudoperonospora spp. Plasmopara spp. Bremia spp. And Bremiella spp. Downy mildew pathogens such as;
Cronartium spp. Melampsora spp. Phakopsora spp. Uredinopsis spp. Blastosporia spp. Gymnosporangium spp. , Puccinia spp. Uromyces spp. , Aecidium spp. Caema spp. , Perdermium spp. , Uredo spp. Phragmidium spp. , Stereostratum spp. Rust pathogens such as; and
Tricholoma matsutake (S. Ito et Imai) Sing. , Tuber spp. Mycorrhizal fungi, such as Suyllus bovines and Rhizopogon rubescens and VA mycorrhiza.
微生物と、その微生物が感染する宿主生物の組み合わせとしては、例えば、以下の組み合わせが挙げられるが、これに限定されない：
オシロイバナ：Ｅｒｙｓｉｐｈｅ ｐｏｌｙｇｏｎｉ ｄｅ Ｃａｎｄｏｌｌｅ
スイトピー：Ｅｒｙｓｉｐｈｅ ｐｉｓｉ ｄｅ Ｃａｎｄｏｌｌｅ
シャクヤク：Ｅｒｙｓｉｐｈｅ ｐａｅｏｎｉａｅ Ｚｈｅｎｇ ｅｔ Ｃｈｅｎ
ケシ類：Ｇｏｌｏｖｉｎｏｍｙｃｅｓ ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ、Ｅｒｙｓｉｐｈｅ ｐｏｌｙｇｏｎｉ ｄｅ Ｃａｎｄｏｌｌｅ、Ｏｉｄｉｕｍ ｓｐ．
ホウズキ：Ｇｏｌｏｖｉｎｏｍｙｃｅｓ ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ
シオン：Ｐｏｄｏｓｐｈａｅｒａ ｆｕｓｃａ（Ｆｒ．）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ
キク：Ｇｏｌｏｖｉｎｏｍｙｃｅｓ ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ
バラ：Ｐｏｄｏｓｐｈａｅｒａ ｐａｎｎｎｏｓａ ｐａｎｎｏｓａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）ｄｅ Ｂａｒｙ
ガーベラ：Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ＆Ｓｈｉｓｈｋｏｆｆ
シネラリア：Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｓｃａ（Ｆｒｉｅｓ：Ｆｒｉｅｓ）Ｂｌｕｍｅｒ ｅｍｅｎｄ．Ｂｒａｕｎ−ｐｒｏ ｐｏｒｔｅ
キョウカノコ ：Ｓｐｈａｅｒｏｔｈｅｃａ ｆｉｌｉｐｅｎｄｕｌａｅ Ｚｈａｏ、Ｓｐｈａｅｒｏｔｈｅｃａ ｈｕｍｕｌｉ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｂｕｒｒｉｌｌ
ホウセンカ：Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ
＆ Ｓｈｉｓｈｋｏｆｆ
ダリア：Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ ＆
Ｓｈｉｓｈｋｏｆｆ
ノイバラ：Ｅｒｙｓｉｐｈｅ ｓｉｍｕｌａｎｓ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｓｉｍｕｌａｎｓ、Ｅｒｙｓｉｐｈｅ ｓｉｍｕｌａｎｓ ｖａｒ． ｔａｎｄａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ
ベゴニア：Ｏｉｄｉｕｍ ｂｅｇｏｎｉａｅ Ｐｕｔｔｅｍａｎｓ ｖａｒ．ｍａｃｒｏｓｐｏｒｕｍ ｄｅ Ｍｅｎｄｏｎｃａ ＆ ｄｅ Ｓｅｑｕｅｉｒａ
ペチュニア：Ｏｉｄｉｕｍ ｓｐ．、Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｓｃａ（Ｆｒｉｅｓ：Ｆｒｉｅｓ）Ｂｌｕｍｅｒ ｅｍｅｎｄ．Ｂｒａｕｎ−ｐｒｏ ｐｏｒｔｅ
宿根カスミソウ：Ｏｉｄｉｕｍ ｓｐ．
カエデ類：Ｓａｗａｄａｅａ ｂｉｃｏｒｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｍｉｙａｂｅ、Ｓａｗａｄａｅａ ｎｅｇｕｎｄｉｎｉｓ Ｈｏｍｍａ、Ｓａｗａｄａｅａ ｐｏｌｙｆｉｄａ ｖａｒ． ｊａｐｏｎｉｃａ Ｂｒａｕｎ ＆Ｔａｎｄａ、Ｓａｗａｄａｅａ ｔｕｌａｓｎｅｉ（Ｆｕｃｋｅｌ）Ｈｏｍｍａ、Ｓａｗａｄａｅａ ｓｐ．、Ｅｒｙｓｉｐｈｅ ｌｉｊｕｂａｒｓｋｉｉ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．、Ｌｉｊｕｂａｒａｓｋｉｉ、Ｐｈｙｌｌａｃｔｉｎｉａ ｃｏｒｙｌｅａ（Ｐｅｒｓｏｏｎ）
Ｋａｒｓｔｅｎ（裏うどんこ病）、Ｐｈｙｌｌａｃｔｉｎｉａ ｓｐ．（裏うどんこ病）
アラカシ：Ｅｒｙｓｉｐｈｅ ｇｒａｃｉｌｉｓ Ｚｈｅｎｇ ＆ Ｃｈｅｎ、Ｅｒｙｓｉｏｈｅ ｈｅｒａｃｌｅｉ ｄｅ Ｃａｎｄｏｌｌｅ、Ｍｉｃｒｏｓｐｈａｅｒａ ｑｕｅｒｃｉ Ｓａｗａｄａ、Ｕｎｃｉｎｕｌａ ｓｐ．、Ｐｈｙｌｌａｃｔｉｎｉａ ｒｏｂｏｒｉｓ（Ｇａｃｈｅｔ）Ｂｌｕｍｅｒ（裏うどんこ病）、Ｃｙｓｔｏｔｈｅｃａ ｗｒｉｇｈｔｉｉ Ｂｅｒｋｅｌｅｙ ＆ Ｃｕｒｔｉｓ（紫かび病）、Ｃｙｓｔｏｔｈｅｃａ ｌａｎｅｓｔｒｉｓ（Ｈａｒｋｎｅｓｓ）Ｍｉｙａｂｅ
シラカシ：Ｅｒｙｓｉｏｈｅ ｈｅｒａｃｌｅｉ ｄｅ Ｃａｎｄｏｌｌｅ、Ｍｉｃｒｏｓｐｈａｅｒａ ｑｕｅｒｃｉ Ｓａｗａｄａ、Ｃｙｓｔｏｔｈｅｃａ ｗｒｉｇｈｔｉｉ Ｂｅｒｋｅｌｅｙ ＆ Ｃｕｒｔｉｓ（紫かび病）
マテバシイ：Ｏｉｄｉｕｍ ｓｐ．
コナラ：Ｍｉｃｒｏｓｐｈａｅｒａ ａｌｐｈｉｔｏｉｄｅｓ Ｇｒｉｆｆｉｔｈｓ ＆
Ｍａｕｂｌａｎｃ、Ｍｉｃｒｏｓｐｈａｅｒａ ｑｕｅｒｃｉ Ｓａｗａｄａ、Ｕｎｃｉｎｕｌａ ｂｉｆｕｒｃａｔａ Ｈｏｍｍａ、Ｕｎｃｉｎｕｌａ ｓｅｐｔａｔａ Ｓａｌｍｏｎ、Ｐｈｙｌｌａｃｔｉｎｉａ ｒｏｂｏｒｉｓ（Ｇａｃｈｅｔ）Ｂｌｕｍｅｒ（裏うどんこ病）、Ｔｙｐｈｕｌｏｃｈａｅｔａ ｊａｐｏｎｉｃａ Ｉｔｏ ＆ Ｈａｒａ（裏うどんこ病）
サクラ類：Ｐｏｄｏｓｐｈａｅｒａ ｌｏｎｇｉｓｅｔａ Ｓａｗａｄａ、Ｐｏｄｏｓｐｈａｅｒａ ｔｒｉｄａｃｔｙｌａ（Ｗａｌｌｒｏｔｈ）ｄｅ Ｂａｒｙ ｖａｒ． ｔｒｉｄａｃｔｙｌａ
サルスベリ：Ｅｒｙｓｉｐｈｅ ａｕｓｔｒａｌｉａｎａ（ＭｃＡｌｐｉｎｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ
ハナカイドウ：Ｐｏｄｏｓｐｈａｅｒａ ｌｅｕｃｏｔｒｉｃｈａ（Ｅｌｌｉｓ ＆ Ｅｖｅｒｈａｒｔ）Ｓａｌｍｏｎ
ハナミズキ：Ｅｒｙｓｉｐｈｅ ｐｕｌｃｈｒａ（Ｃｏｏｋ ｅｔ Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ． ｐｕｌｃｈｒａ
モミジ類：カエデ類と同一
ヤナギ類：Ｕｎｃｉｎｕｌａ ａｄｕｎｃａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ． ａｄｕｎｃａ、Ｕｎｃｉｎｕｌａ ａｄｕｎｃａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ． ｍａｎｄｓｈｕｒｉｃａ（Ｍｉｕｒａ）Ｚｈｅｎｇ ＆ Ｃｈｅｎ、Ｕｎｃｉｎｕｌａ ｌｏｎｇｉｓｐｏｒａ Ｚｈｅｎｇ ＆
Ｃｈｅｎ ｖａｒ． ｍｉｎｏｒ Ｚｈｅｎｇ ＆ Ｃｈｅｎ
アオキ：Ｍｉｃｒｏｓｐｈａｅｒａ ｓｐ．、Ｏｉｄｉｕｍ ｓｐ．
ヒイラギナンテンＭｉｃｒｏｓｐｈａｅｒａ ｂｅｒｂｅｒｉｄｉｃｏｌａ Ｔａｉ
サルスベリ：Ｅｒｙｓｉｐｈｅ ａｕｓｔｒａｌｉａｎａ（ＭｃＡｌｐｉｎｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ
イチゴ：Ｐｏｄｏｓｐｈａｅｒａ ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆
Ｔａｋａｍａｔｓｕ ｖａｒ． ａｐｈａｎｉｓ
キュウリ：Ｐｏｄｏｓｐｈａｅｒａ ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ。
Examples of a combination of a microorganism and a host organism that the microorganism infects include, but are not limited to, the following combinations:
Osiroybana: Erysiphe polygoni de Candole
Sweetpy: Erysiphe pisi de Candole
Peonies: Erysiphe paeoniae Zheng et Chen
Poppies: Golovinomyces cichoacearum (de Candole) Geljuta, Erysiphe polygoni de Candole, Oidium sp.
Houki: Golovinomycins cichoracerarum (de Candole) Geljuta
Zion: Podospherea fusca (Fr.) Braun & Shishoff
Chrysanthemum: Golovinomyces cicioacearum (de Candole) Geljuta
Rose: Podospherea pannosa pannosa (Wallroth: Fries) de Barry
Gerbera: Podophaera xanthii (Castagne) Braun & Shishkoff
Cineraria: Sphaerotheca fusca (Fries: Fries) Blumer emend. Braun-pro porte
Kyokanoko: Sphaerotheca filipendulae Zhao, Sphaerotheca humuli (de Candle) Burrill
Spinach: Podospherea xanthii (Castagne) Braun
& Shishkoff
Dahlia: Podospherea xanthii (Castagne) Braun &
Shishkoff
Neuborough: Erysiphe simulans (Salmon) Braun & Takamatsu var. simulans, Erysiphe simulans var. tandae (Braun) Braun & Takamatsu
Begonia: Oidium begonia Putmans var. macrosporum de Mendonca & de Sequeira
Petunia: Oidium sp. Sphaerotheca fusca (Fries: Fries) Blumer emend. Braun-pro porte
Perennial gypsophila: Oidium sp.
Maples: Sawadaea bicornis (Walloth) Miyabe, Sawadaea negundinis Homa, Sawadaea polyfida var. japonica Braun & Tanda, Sawadaea tulasnei (Fuckel) Homa, Sawadaea sp. Erysiphe lijubarskii Braun & Takamatsu var. , Lijubaraskiii, Phyllactinia corelea (Person)
Karsten (back powdery mildew), Phyllactinia sp. (Back powdery mildew)
Aracasi: Erysiphe gracilis Zheng & Chen, Erysiohe herculei de Candole, Microsphaera querci Sawada, Uncinula sp. , Phyllactinia robotis (Gachet) Blumer (back powdery mildew), Cytotheca wrighthii Berkeley & Curtis (purple mold), Cystotheca lantris (Harkness) Myabe
Shirakashi: Erysiohe herculei de Candole, Microsphaera querci Sawada, Cytotheca wrightii Berkeley & Curtis (purple mold disease)
Matebashii: Oidium sp.
Konara: Microsphere alfitoides Griffiths &
Maublanc, Microsphaera quarci Sawada, Uncinula bifurcata Homma, Uncinula septa Salmon, Phyllactinia robois (Gachet) Blumer (Back)
Cherry: Podophaera longiseta Sawada, Podophaera triactyla (Walloth) de Barry var. triactyla
Crape myrtle: Erysiphe australiana (McAlpine) Braun & Takamatsu
Hanakaidou: Podospherea leukotricha (Ellis & Everhart) Salmon
Dogwood: Erysiphe pulchra (Cook et Peck) Braun & Takamatsu var. pulchra
Maples: Same willow as maple: Uncinula aduna (Wallroth: Fries) Levelil var. adunca, Uncinula adunca (Wallroth: Fries) Levelil var. manshuria (Miura) Zheng & Chen, Uncinula longisspora Zheng &
Chen var. minor Zheng & Chen
Aoki: Microsphaera sp. Oidium sp.
Holly Nanten Microsphaera berberdicola Tai
Crape myrtle: Erysiphe australiana (McAlpine) Braun & Takamatsu
Strawberry: Podospherea aphanis (Wallroth) Braun &
Takamatsu var. aphanis
Cucumber: Podospherea xanthii (Castagne) Braun & Shishoff.
本発明において使用する場合、「共生者」とは、微生物と共生する生物をいい、植物および動物が挙げられるが、これに限定されない。 As used in the present invention, a “symbiotic person” refers to an organism that coexists with microorganisms, including but not limited to plants and animals.
本発明において使用する場合、「宿主生物」とは、本発明の微生物が感染し得る細胞を意味する。宿主細胞としては、植物および動物が挙げられるが、これに限定されない。 As used in the present invention, “host organism” means a cell that can be infected by the microorganism of the present invention. Host cells include but are not limited to plants and animals.
本明細書において用いられる「植物」とは、植物界に属する生物の総称であり、クロロフィル、かたい細胞壁、豊富な永続性の胚的組織の存在，および運動する能力がない生物により特徴付けられる。代表的には、植物は、細胞壁の形成・クロロフィルによる同化作用をもつ顕花植物をいう。「植物」は、単子葉植物および双子葉植物のいずれも含む。好ましい植物としては、樹木、芝生、雑草などが挙げられ、例えば、シロイヌナズナ、アブラナおよびヒマワリが挙げられるがそれらに限定されない。本発明の植物は、コムギ、トウモロコシ、イネ、オオムギ、ソルガムなどのイネ科に属する単子葉植物、、タバコ、ピーマン、ナス、メロン、トマト、サツマイモ、キャベツ、ネギ、ブロッコリー、ニンジン、キウリ、柑橘類、白菜、レタス、モモ、ジャガイモおよびリンゴであってもよい。あるいは、本発明の植物は、オシロイバナ、スイートピー、シャクヤク、ケシ類ホウズキシオン、キク、バラ、ガーベラジニアシネラリア、キョウカノコ、ホウセンカ、ダリア、バラ、ベゴニア、ペチュニア、宿根カスミソウ、カエデ、アラカシ、シラカシ、マテバシイ、コナラ、サクラ、サルスベリ、ハナカイドウ、ハナミズキ、モミジ、ヤナギ、アオキ、ヒイラギナンテン、サルスベリ、ベコニア、モミジ、イチゴ、および、キュウリであってもよい。好ましい植物は樹木、芝生、雑草などに限られず、花、作物も含まれる。特に他で示さない限り、植物は、植物体、植物器官、植物組織、植物細胞、および種子のいずれをも意味する。植物器官の例としては、根、葉、茎、および花などが挙げられる。植物細胞の例としては、カルスおよび懸濁培養細胞が挙げられる。特定の実施形態では、植物は、植物体を意味し得る。 As used herein, “plant” is a general term for organisms belonging to the plant kingdom, characterized by chlorophyll, hard cell walls, the presence of abundant and permanent embryonic tissue, and organisms that are not capable of motility. . Typically, a plant refers to a flowering plant having cell wall formation and anabolism by chlorophyll. “Plant” includes both monocotyledonous and dicotyledonous plants. Preferable plants include trees, lawns, weeds, and the like, and examples include, but are not limited to, Arabidopsis thaliana, rape and sunflower. The plant of the present invention is a monocotyledonous plant belonging to the family Gramineae such as wheat, corn, rice, barley, sorghum, tobacco, peppers, eggplant, melon, tomato, sweet potato, cabbage, leek, broccoli, carrot, cucumber, citrus, Chinese cabbage, lettuce, peach, potato and apple may be used. Alternatively, the plant of the present invention is oyster loaf, sweet pea, peonies, poppy hoe squirrel, chrysanthemum, rose, gerbera cinellaria, mushroom, spinach, dahlia, rose, begonia, petunia, perennial gypsophila, maple, arakashi, shirakashi, matebashii, It may be konara, cherry blossom, crape myrtle, red coral, dogwood, maple, willow, aoki, holly nanten, crape myrtle, beconia, maple, strawberry, and cucumber. Preferred plants are not limited to trees, lawns, weeds, etc., but also include flowers and crops. Unless otherwise indicated, a plant means any plant, plant organ, plant tissue, plant cell, and seed. Examples of plant organs include roots, leaves, stems and flowers. Examples of plant cells include callus and suspension culture cells. In certain embodiments, a plant can mean a plant.
別の実施形態において、本発明において使用され得る植物種の例としては、ナス科、イネ科、アブラナ科、バラ科、マメ科、ウリ科、シソ科、ユリ科、アカザ科、セリ科、イネ科、アブラナ科の植物が挙げられる。 In another embodiment, examples of plant species that can be used in the present invention include solanaceae, gramineous, cruciferous, rosaceae, legumes, cucurbitaceae, perillaceae, liliaceae, red crustaceae, ciliaceae, And plants of the family Brassicaceae.
アブラナ科の植物の例としては、Ｒａｐｈａｎｕｓ、Ｂｒａｓｓｉｃａ、Ａｒａｂｉｄｏｐｓｉｓ、Ｗａｓａｂｉａ、またはＣａｐｓｅｌｌａに属する植物が挙げられ、例えば、大根、アブラナ、シロイヌナズナ、ワサビ、ナズナなどを含む。 Examples of cruciferous plants include plants belonging to Raphanus, Brassica, Arabidopsis, Wasabia, or Capsella, and include, for example, radish, rape, Arabidopsis thaliana, horseradish, and tuna.
イネ科の植物の例としては、Ｏｒｙｚａ、Ｈｏｒｄｅｎｕｍ、Ｓｅｃａｌｅ、Ｓｃｃｃｈａｒｕｍ、Ｅｃｈｉｎｏｃｈｌｏａ、またはＺｅａに属する植物が挙げられ、例えば、イネ、オオムギ、ライムギ、ヒエ、モロコシ、トウモロコシなどを含む。 Examples of gramineous plants include plants belonging to Oryza, Hordenum, Secale, Scccharum, Echinochloa, or Zea, and include, for example, rice, barley, rye, millet, sorghum, corn, and the like.
木本植物の例としては、ポプラ、コーヒー、スギ、ヒノキ、ユーカリ、マングローブなどが挙げられるがそれらに限定されない。 Examples of woody plants include, but are not limited to, poplar, coffee, cedar, cypress, eucalyptus, mangrove and the like.
本明細書において「動物」は、当該分野において最も広義で用いられ、脊椎動物および無脊椎動物を含む。動物としては、哺乳綱、鳥綱、爬虫綱、両生綱、魚綱、昆虫綱、蠕虫綱などが挙げられるがそれらに限定されない。 As used herein, “animal” is used in the broadest sense in the art and includes vertebrates and invertebrates. Examples of animals include, but are not limited to, mammals, birds, reptiles, amphibians, fishes, insects, and worms.
本明細書において、生物の「組織」とは、細胞の集団であって、その集団において一定の同様の作用を有するものをいう。好ましくは、本発明の「組織」とは、本発明の微生物が感染および／または共生する部分を含む。従って、組織は、臓器（器官）の一部であってもよい。また、本発明の方法において、微生物を保存するために使用される「組織」として、生物全体または器官全体を使用してもよい。臓器（器官）内では、同じ働きを有する細胞を有することが多いが、微妙に異なる働きを有するものが混在することもあることから、本明細書において組織は、一定の特性を共有する限り、種々の細胞を混在して有していてもよい。 In the present specification, the “tissue” of an organism refers to a group of cells having a certain similar action in the group. Preferably, the “tissue” of the present invention includes a part where the microorganism of the present invention is infected and / or symbiotic. Therefore, the tissue may be a part of an organ (organ). In the method of the present invention, the whole organism or whole organ may be used as the “tissue” used for storing the microorganism. In an organ (organ), it often has cells having the same function, but those having slightly different functions may be mixed, so in the present specification, as long as the tissue shares certain characteristics, Various cells may be mixed.
本明細書において、「器官（臓器）」とは、１つ独立した形態をもち、１種以上の組織が組み合わさって特定の機能を営む構造体を形成したものをいう。植物では、カルス、根、茎、幹、葉、花、種子、胚芽、胚、果実などが挙げられるがそれらに限定されない。動物では、胃、肝臓、腸、膵臓、肺、気管、鼻、心臓、動脈、静脈、リンパ節（リンパ管系）、胸腺、卵巣、眼、耳、舌、皮膚等が挙げられるがそれらに限定されない。 In the present specification, an “organ” refers to a structure having one independent form and a structure that performs a specific function by combining one or more tissues. Examples of the plant include, but are not limited to, callus, root, stem, stem, leaf, flower, seed, germ, embryo, and fruit. Animals include, but are not limited to, stomach, liver, intestine, pancreas, lung, trachea, nose, heart, artery, vein, lymph node (lymphatic system), thymus, ovary, eye, ear, tongue, skin, etc. Not.
（乾燥処理をする組織などの調製）
乾燥処理をする対象としては、保存対象の微生物が感染した組織、保存対象の微生物と共生者との共生体、保存対象の微生物とその微生物が感染することができる宿主生物との混合物、および、保存対象の微生物とその微生物が共生することができる共生者との混合物が挙げられるが、これに限定されない。好ましくは、本発明において乾燥する対象となるのは、保存対象の微生物が感染した組織、および、保存対象の微生物と共生者との共生体である。
(Preparation of tissues to be dried)
The target to be dried is a tissue infected with a microorganism to be preserved, a symbiosis between the microorganism to be preserved and a symbiotic person, a mixture of the microorganism to be preserved and a host organism to which the microorganism can be infected, and Examples include, but are not limited to, a mixture of a microorganism to be stored and a symbiotic person with whom the microorganism can coexist. Preferably, the target to be dried in the present invention is a tissue infected with a microorganism to be stored, and a symbiosis of the microorganism to be stored and a symbiotic person.
必要に応じて乾燥処理をする組織、共生体、混合物（以下、組織等）を、小片にする。本発明における「小片」とは、その大きさが厳密に規定されるものではないが、出発材料よりも小さな大きさに破壊・切断等された物質を小片という。小片にすることによって、乾燥工程の効率が上昇し、かつ、凍結工程において、組織内部・共生体内部での温度差が小さくなるという利点がある。小片にする手段としては、機械的に組織等を破壊する手段が含まれ、例えば、ナイフ・はさみなどで、組織等を切断することがが挙げられるが、これに限定されない。好ましい小片の大きさは、乾燥時間に依存して変化する。一般的には、より長時間の乾燥をする場合は、より大きな小片を用いる。用いる小片の大きさとしては、一般的には、５〜１０ｍｍ×５〜１０ｍｍ、例えば、１０ｍｍ×７ｍｍ、７ｍｍ×５ｍｍ、および、１０ｍｍ×１０ｍｍなどが挙げられるが、これに限定されない。 A tissue, a symbiosis, or a mixture (hereinafter referred to as a tissue or the like) to be subjected to a drying treatment as necessary is made into small pieces. The “small piece” in the present invention is not strictly defined in size, but a substance destroyed or cut to a smaller size than the starting material is called a small piece. By using small pieces, there is an advantage that the efficiency of the drying process is increased and the temperature difference between the inside of the tissue and the inside of the symbiosis is reduced in the freezing process. The means for making a small piece includes a means for mechanically destroying the tissue or the like, and for example, cutting the tissue or the like with a knife or scissors can be mentioned, but is not limited thereto. The preferred piece size varies depending on the drying time. In general, larger pieces are used for longer drying times. Generally as a magnitude | size of the small piece to be used, although 5-10 mm x 5-10 mm, for example, 10 mm x 7 mm, 7 mm x 5 mm, 10 mm x 10 mm etc. are mentioned, it is not limited to this.
（組織の乾燥方法）
本発明の方法において組織を乾燥するためには、周知の種々の方法を使用することが可能である。限定されることはないが、本発明の乾燥工程は、例えば、乾燥剤の存在下で組織を放置することよって行うことも可能である。そのような乾燥剤としては、例えば、酸化アルミニウム、カルシウム、塩化カルシウム、水素化カルシウム、酸化カルシウム、硫酸カルシウム、硫酸銅、乾燥剤小袋、水素化リチウムアルミニウム、マグネシウム、酸化マグネシウム、過塩素酸マグネシウム、硫酸マグネシウム、モレキュラーシーブ、五酸化リンおよびシカペント、炭酸カリウム、水酸化カリウム、シリカゲル（例えば、ブルーゲル、オレンジゲル、ブラウンゲルなど）、ナトリウム、水酸化ナトリウム、硫酸ナトリウム、ならびに、硫酸およびシカサイドが挙げられるが、これに限定されない。好ましい乾燥剤は、塩化カルシウムである。
(Tissue drying method)
Various known methods can be used to dry the tissue in the method of the present invention. Although not limited, the drying step of the present invention can be performed, for example, by leaving the tissue in the presence of a desiccant. Examples of such a desiccant include aluminum oxide, calcium, calcium chloride, calcium hydride, calcium oxide, calcium sulfate, copper sulfate, desiccant sachets, lithium aluminum hydride, magnesium, magnesium oxide, magnesium perchlorate, Examples include magnesium sulfate, molecular sieve, phosphorus pentoxide and cicapent, potassium carbonate, potassium hydroxide, silica gel (eg, blue gel, orange gel, brown gel, etc.), sodium, sodium hydroxide, sodium sulfate, and sulfuric acid and decaside. However, it is not limited to this. A preferred desiccant is calcium chloride.
乾燥剤存在下に組織を放置する場合、代表的には室温（例えば、１５〜２５℃）で行う。乾燥剤存在下に組織を放置する時間は、当業者が適宜選択することが可能である。一般的には、乾燥時間は、小片の大きさに依存する。例えば、５〜７ｍｍ×５〜１０ｍｍの小片を用いる場合は、３時間〜１２時間、好ましくは、３時間〜６時間の乾燥を行う。実際に３〜１２時間乾燥した場合に、凍結保存後でも菌の分生子の発芽が確認された。 When the tissue is left in the presence of a desiccant, it is typically performed at room temperature (for example, 15 to 25 ° C.). A person skilled in the art can appropriately select the time for leaving the tissue in the presence of the desiccant. In general, the drying time depends on the size of the pieces. For example, when a small piece of 5 to 7 mm × 5 to 10 mm is used, drying is performed for 3 hours to 12 hours, preferably 3 hours to 6 hours. When actually dried for 3 to 12 hours, germination of fungal conidia was confirmed even after cryopreservation.
（乾燥した組織の凍結方法）
上記乾燥工程において乾燥した組織を、凍結する工程としては、以下：
（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）予備凍結することなく、乾燥組織を直接液体窒素中で−１６５℃で凍結する工程が挙げられるが、これに限定されない。
(Dry tissue freezing method)
The process of freezing the tissue dried in the drying process is as follows:
(I) pre-freezing at −70 ° C. to −80 ° C. followed by freezing in liquid nitrogen at −165 ° C .; and (ii) drying tissue directly in liquid nitrogen at −165 ° C. without pre-freezing Although the process of freezing is mentioned, it is not limited to this.
予備凍結法としては、例えば：
（ａ）プログラムフリーザーを用いる、約−１℃／分の一定速度による−７０℃〜−８０℃までの予備凍結；
（ｂ）−７０℃〜−８０℃においてフリージングコンテナ（例えば、ミスターフロステー，Ｎａｌｇｅｎｅ社製：１分間に約１℃低下する）を用いる予備凍結；および
（ｃ）ドライアイスでの予備凍結（必要に応じてエタノールのようなアルコールを添加する）、が挙げられるが、これに限定されない。これら予備凍結および凍結保存においては、組織小片を、例えば、セラムチューブにいれてもよい。
Examples of pre-freezing methods include:
(A) pre-freezing from -70 ° C to -80 ° C at a constant rate of about -1 ° C / min using a program freezer;
(B) pre-freezing with a freezing container (eg Mr. Frostay, Nalgene: reduced by about 1 ° C. per minute) at −70 ° C. to −80 ° C .; and (c) pre-freezing with dry ice (necessary) Depending on the case, an alcohol such as ethanol is added), but is not limited thereto. In these preliminary freezing and cryopreservation, the tissue piece may be placed in a serum tube, for example.
（凍結した組織の解凍処理）
上記凍結工程によって凍結された組織を、長期保存後に解凍する。解凍温度は、１０〜７０℃、好ましくは２０〜６０℃、より好ましくは３０〜５０℃であるが、これらに限定されない。
(Thaw processing of frozen tissue)
The tissue frozen by the freezing step is thawed after long-term storage. The thawing temperature is 10 to 70 ° C, preferably 20 to 60 ° C, more preferably 30 to 50 ° C, but is not limited thereto.
解凍時間は、１０秒〜５分間、好ましくは３０〜３分間、より好ましくは約１分間であるが、これらに限定されない。 The thawing time is 10 seconds to 5 minutes, preferably 30 to 3 minutes, more preferably about 1 minute, but is not limited thereto.
解凍後の微生物を、宿主生物または共生者（好ましくは、本発明の方法において使用した宿主生物または共生者と同一種）に接触および／または接種し、そして、増殖させる。 The thawed microorganism is contacted and / or inoculated with a host organism or symbiotic person (preferably the same species as the host organism or symbiotic person used in the method of the present invention) and allowed to grow.
本発明を実施例により説明する。以下の実施例は、本発明の例示であって、本発明を制限するものではない。 The present invention is illustrated by examples. The following examples are illustrative of the present invention and are not intended to limit the present invention.
（実施例１：プログラムフリーザでの予備凍結を用いる、組織の凍結保存）
（１．組織の凍結保存）
本発明の方法に従って、以下のように、キュウリうどんこ病（Ｐｏｄｏｓｐｈａｅｒａ
ｘａｎｈｔｉｉ （Ｃａｓｔａｇｎｅ） Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ）に感染した組織を凍結した。
(Example 1: Tissue cryopreservation using pre-freezing in a program freezer)
(1. Cryopreservation of tissue)
In accordance with the method of the present invention, cucumber powdery mildew (Podophaera) is as follows:
Tissue infected with xanhti (Castagne) Braun & Shishoff) was frozen.
キュウリうどんこ病に感染したキュウリ（品種ときわ光３号 Ｃｕｃｕｍｉｓｓａｔｉｖｕｓ Ｌ．）の発病葉を、５〜７ｍｍ×５〜１０ｍｍの小片に切断した。この小片を、
塩化カルシウム２５ｇをいれた直径９ｃｍのシャーレに封入して、室温にて、各々、１時間、３時間、および６時間乾燥させた。その後、乾燥組織を、セラムチューブに入れ、プログラムフリーザを用いて、約１−℃／分の速度で−８０℃まで冷却し、予備凍結をした後、−１６５℃の液体窒素気相中で、３ヶ月間、８ヶ月間、１２ヶ月および１５ヶ月間、凍結保存した。
The diseased leaves of cucumber infected with cucumber powdery mildew (variety Tokiwamitsu No. 3 Cucumisativus L.) were cut into small pieces of 5 to 7 mm × 5 to 10 mm. This small piece,
25 g of calcium chloride was sealed in a petri dish having a diameter of 9 cm and dried at room temperature for 1 hour, 3 hours, and 6 hours, respectively. Thereafter, the dried tissue is placed in a serum tube, cooled to −80 ° C. at a rate of about 1 ° C./min using a program freezer, pre-frozen, and then in a liquid nitrogen gas phase at −165 ° C. Cryopreserved for 3 months, 8 months, 12 months and 15 months.
（２．凍結組織の解凍）
実施例１にて凍結した組織を、保存開始から３ヵ月後、８ヵ月後、１年後および１年３ヶ月後に解凍した。具体的には、凍結保存した組織を、セラムチューブごと約５０℃のウォーターバス中につけて、約１分間解凍処理を行った。解凍処理を行った組織小片の分生子を、健全な（うどんこ病に感染していない）キュウリ子葉に直接接触させて接種した。うどんこ病の病原菌を増殖させ、分生子の発芽率を観察した。その結果を、図１に示す。実施例１での乾燥時間が１時間のサンプルを「▲」で、３時間のサンプルを「■」で、６時間のサンプルを「◆」で示した。
(2. Thawing frozen tissue)
The tissue frozen in Example 1 was thawed 3 months, 8 months, 1 year and 1 year and 3 months after the start of storage. Specifically, the cryopreserved tissue was placed in a water bath at about 50 ° C. together with the serum tube, and thawed for about 1 minute. The conidia of the tissue pieces that had been thawed were inoculated by direct contact with healthy cucumber cotyledons (not infected with powdery mildew). The pathogenic bacteria of powdery mildew were grown and the germination rate of conidia was observed. The result is shown in FIG. In Example 1, the sample with a drying time of 1 hour is indicated by “▲”, the sample for 3 hours is indicated by “■”, and the sample for 6 hours is indicated by “♦”.
この結果に示されるように、人工培養が不可能な微生物であっても、発芽率に示されるような生物学的活性を損なうことなく、１年間にわたり長期保存が可能である。また、保存開始から１年後であっても分生子の発芽率は２０％以上であった。また、分生子の発芽とキュウリ子葉への感染が確認された。また、この結果は、感染組織を５〜７ｍｍ×５〜１０ｍｍの小片に切断した場合には、乾燥時間は１時間では不十分であったことを示した。１年間凍結保存したキュウリうどんこ病のキュウリ葉上での菌糸生育を顕微鏡にて確認した。 As shown in this result, even a microorganism that cannot be artificially cultured can be stored for a long period of time for one year without impairing the biological activity as indicated by the germination rate. Further, even after one year from the start of storage, the conidia germination rate was 20% or more. In addition, germination of conidia and infection of cucumber cotyledons were confirmed. Moreover, this result showed that when the infected tissue was cut into small pieces of 5 to 7 mm × 5 to 10 mm, a drying time of 1 hour was insufficient. The mycelial growth on cucumber leaves of cucumber powdery mildew that had been cryopreserved for one year was confirmed with a microscope.
さらに、３時間乾燥した組織および６時間乾燥した組織を用いて１年３ヶ月凍結保存したキュウリうどんこ病菌を、健全なキュウリ子葉に直接接触させて接種し、キュウリうどんこ病菌がキュウリ子葉上に生育することを確認した。接種１０日後のキュウリ子葉の写真を、それぞれ、図２および図３に示す。 Furthermore, cucumber powdery mildew frozen for 1 year and 3 months using a tissue dried for 3 hours and a tissue dried for 6 hours was inoculated directly in contact with a healthy cucumber cotyledon, and the cucumber powdery mildew was inoculated on the cucumber cotyledon. It was confirmed to grow. Photographs of cucumber cotyledons 10 days after inoculation are shown in FIGS. 2 and 3, respectively.
（実施例２：プログラムフリーザーを用いない、組織の凍結保存）
（１．フリージングコンテナでの予備凍結を用いる組織の凍結保存）
プログラムフリーザーを用いる代わりに、フリージングコンテナでの予備凍結を用いた。具体的には、うどんこ病罹病キュウリ葉を、約７ｍｍ幅、長さ約７ｍｍの小片にし、２．０ｍｌのセラムチューブに入れ、乾燥処理後、フリージングコンテナ（ミスターフロステー，Ｎａｌｇｅｎｅ社製）に入れて約-７０℃の冷凍庫にて予備凍結、１夜経過後、液
体窒素気相（約−１６０℃）内で凍結保存した。実施例１の場合と同様に、凍結組織を解凍した。
(Example 2: Cryopreservation of tissue without using a program freezer)
(1. Cryopreservation of tissue using pre-freezing in a freezing container)
Instead of using a program freezer, pre-freezing in a freezing container was used. Specifically, powdery mildew-affected cucumber leaves are made into small pieces of about 7 mm width and length of about 7 mm, placed in a 2.0 ml serum tube, dried, and then put into a freezing container (Mr. Frostay, manufactured by Nalgene). The sample was pre-frozen in a freezer at about -70 ° C, and after one night, stored frozen in a liquid nitrogen gas phase (about -160 ° C). The frozen tissue was thawed as in Example 1.
（２．予備凍結を用いない組織の凍結保存）
予備凍結を用いずに、感染組織を凍結保存した。具体的には、うどんこ病罹病キュウリ葉を、約７ｍｍ幅、長さ約７ｍｍの小片にし、２．０ｍｌのセラムチューブに入れ、乾燥処理後、液体窒素気相（約−１６０℃）内で凍結保存した。実施例１の場合と同様に、凍結組織を解凍した。
(2. Cryopreservation of tissue without pre-freezing)
The infected tissue was stored frozen without using pre-freezing. Specifically, powdery mildew-affected cucumber leaves are cut into small pieces having a width of about 7 mm and a length of about 7 mm, placed in a 2.0 ml serum tube, dried, and then in a liquid nitrogen gas phase (about −160 ° C.). Cryopreserved. The frozen tissue was thawed as in Example 1.
（３．フリージングコンテナでの予備凍結を用いる組織の凍結保存法と、予備凍結を用いない組織の凍結保存法との比較）
（３．１．乾燥条件の凍結保存効率に対する影響）
プログラムフリーザーを用いなくても、本発明の方法が実施可能であり、かつ、本発明の方法が乾燥時の環境の影響を受けないことを実証するために、異なる乾燥条件下で、３時間および６時間乾燥した組織を凍結保存し、解凍後に分生子の発芽率を検証した。
(3. Comparison between tissue cryopreservation method using pre-freezing in freezing container and tissue cryopreservation method without pre-freezing)
(3.1. Effect of drying conditions on cryopreservation efficiency)
To demonstrate that the method of the present invention can be carried out without a program freezer and that the method of the present invention is not affected by the environment during drying, under different drying conditions for 3 hours and The tissue dried for 6 hours was stored frozen, and the conidia germination rate was verified after thawing.
具体的には、約７ｍｍ×約７ｍｍの感染組織小片を調製し、その小片５枚、または１５枚を、２５ｇの塩化カルシウムとともに、セラムチューブに入れた。乾燥処理時の小片の枚数が多ければ多いほど、乾燥条件は、より緩やかになる。予備凍結をする場合は、そのセラムチューブを室温に保存したフリージングコンテナに入れ、−７０℃〜−８０℃オーバーナイト維持した後、セラムチューブを液体窒素中で凍結保存した（表中の「フリージングコンテナ」）。予備凍結しない場合は、乾燥後のセラムチューブを直接液体窒素中に入れた（表中の「直接液体窒素」）。対照として、凍結処理をしない場合の発芽率を検討した（表中の「なし」）。その結果を、以下の表１および表２に示す。 Specifically, infected tissue pieces of about 7 mm × about 7 mm were prepared, and 5 or 15 pieces of the pieces were placed in a serum tube together with 25 g of calcium chloride. The more the number of small pieces during the drying process, the milder the drying conditions. In the case of pre-freezing, the serum tube was put in a freezing container stored at room temperature and kept at -70 ° C to -80 ° C overnight, and then the serum tube was stored frozen in liquid nitrogen (see “Freezing container in the table”). "). When it was not pre-frozen, the dried serum tube was directly placed in liquid nitrogen (“direct liquid nitrogen” in the table). As a control, the germination rate without freezing was examined (“None” in the table). The results are shown in Table 1 and Table 2 below.
（３．２．乾燥時間の凍結保存効率に対する影響）
次に、種々の時間を検討した。具体的には、乾燥時間を、０時間、１時間、３時間、１２時間とした以外は、上記３．１．と同一の条件で、実験を行った。その結果を表３および表４に示す。
(3.2. Effect of drying time on cryopreservation efficiency)
Next, various times were examined. Specifically, the above 3.1. Except that the drying time was 0 hour, 1 hour, 3 hours, and 12 hours. The experiment was performed under the same conditions as those described above. The results are shown in Tables 3 and 4.
（実施例３：微生物感染組織から単離した分生子を用いる凍結保存法） 接種２週間後程度のうどんこ病罹病キュウリ葉から、筆で払い落とす方法で分生子を採集し、セラムチューブに入れてサンプルとした。採集直後にサンプルを炭酸カルシウムの入ったシャーレ中で１、３、６時間乾燥処理後、フリージングコンテナを用いて予備凍結し、液体窒素気相中で凍結保存した。１週間保存後、取り出して50℃のお湯中で約１分間解凍処理後、タマネギ鱗片表皮に接種し、約一日後に発芽率を観察し、生存率を評価した。 (Example 3: Cryopreservation method using conidia isolated from a microorganism-infected tissue) Conidia were collected from a cucumber leaf diseased with powdery mildew about 2 weeks after inoculation by brushing off with a brush and placed in a serum tube. Sample. Immediately after collection, the sample was dried in a petri dish containing calcium carbonate for 1, 3, 6 hours, pre-frozen using a freezing container, and stored frozen in a liquid nitrogen gas phase. After storage for 1 week, the sample was taken out and thawed in hot water at 50 ° C. for about 1 minute, and then inoculated into the onion scale epidermis. After about one day, the germination rate was observed to evaluate the survival rate.
キュウリうどんこ病菌の分生子だけの凍結保存法について検討した結果（表５）、乾燥処理０時間（対照区）、１時間、３時間、６時間後に液体窒素気相中で保存した後の分生子の発芽率は、それぞれ10.1％〜18.6％（平均14.8％）、21.4％〜42.1％（平均30.1％）、3.2％〜11.1％（平均7.3％）、4.5％〜11.3％（平均7.2％）と、乾燥処理１時間の場合に、分生子の生存が良好であり、かつ対照区よりも高い発芽率を示し、分生子のみでも保存可能であることが明らかになった Results of examination of cryopreservation method only for conidia of cucumber powdery mildew fungus (Table 5), minutes after storage in liquid nitrogen gas phase after 0 hours (control group), 1 hour, 3 hours and 6 hours of drying treatment The germination rates of the offspring are 10.1% to 18.6% (average 14.8%), 21.4% to 42.1% (average 30.1%), 3.2% to 11.1% (average 7.3%), 4.5% to 11.3% (average 7.2%) In the case of the drying treatment for 1 hour, it was revealed that the conidia survived well, showed a higher germination rate than the control group, and could be stored with only conidia.
以上のように、本発明の好ましい実施形態を用いて本発明を例示してきたが、本発明は、この実施形態に限定して解釈されるべきものではない。本発明は、特許請求の範囲によってのみその範囲が解釈されるべきであることが理解される。当業者は、本発明の具体的な好ましい実施形態の記載から、本発明の記載および技術常識に基づいて等価な範囲を実施することができることが理解される。本明細書において引用した特許、特許出願および文献は、その内容自体が具体的に本明細書に記載されているのと同様にその内容が本明細書に対する参考として援用されるべきであることが理解される。 As mentioned above, although this invention has been illustrated using preferable embodiment of this invention, this invention should not be limited and limited to this embodiment. It is understood that the scope of the present invention should be construed only by the claims. It is understood that those skilled in the art can implement an equivalent range based on the description of the present invention and the common general technical knowledge from the description of specific preferred embodiments of the present invention. Patents, patent applications, and documents cited herein should be incorporated by reference in their entirety, as if the contents themselves were specifically described herein. Understood.
本発明に従って、人工培養が不可能な微生物を長期に保存することが可能となる。従って、本発明は、微生物、特に感染能を有する微生物の解析などを行う農学および植物病理学などの分野において利用可能である。 According to the present invention, microorganisms that cannot be artificially cultured can be stored for a long time. Therefore, the present invention can be used in fields such as agriculture and plant pathology for analyzing microorganisms, particularly microorganisms having infectivity.
Claims (19)
ａ）微生物が感染した宿主生物の組織を乾燥する工程；および
ｂ）該乾燥組織を凍結する工程、
を包含する、方法であって、
ここで、該（ａ）乾燥工程が乾燥剤存在下において室温で３時間以上行われ、
該（ｂ）凍結する工程が、以下：
（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）液体窒素中で−１６５℃で凍結する工程
からなる群から選択され、
該微生物が、うどんこ病の病原菌、べと病のべと病菌、さび病のさび病菌、および、菌根菌からなる群から選択され、該宿主生物が、該病原菌に感染した植物である、方法。 A method for preserving microorganisms comprising:
a) drying a tissue of a host organism infected with microorganisms; and b) freezing the dried tissue;
A method comprising :
Here, the (a) drying step is performed at room temperature for 3 hours or more in the presence of a desiccant,
The step (b) freezing is as follows:
(I) pre-freezing at -70 ° C to -80 ° C and then freezing at -165 ° C in liquid nitrogen; and
(Ii) freezing at −165 ° C. in liquid nitrogen
Selected from the group consisting of
The microorganism is selected from the group consisting of powdery mildew pathogen, downy mildew fungus, rust rust fungus, and mycorrhizal fungus, and the host organism is a plant infected with the pathogen, Way .
ｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヒメヤシャブシうどんこ病］、ａｕｓｔｒａｌｉａｎａ（ＭｃＡｌｐｉｎｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サルスベリうどんこ病］、ｂｅｔｕｌｉｎａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［カンバ類うどんこ病］、ｂｉｆｕｒｃａｔａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コナラうどんこ病］、ｂｕｃｋｌｅｙａｅ（Ｎｏｍｕｒａ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツクバネうどんこ病］、ｃａｒｐｉｎｉｃｏｌａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シデ類うどんこ病］、ｃｅｄｒｅｌａｅ（Ｔａｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チャンチンうどんこ病］、ｃｌａｎｄｅｓｔｉｎａ Ｂｉｖｏｎａ−Ｂｅｒｎａｒｄｉ［ニレ類うどんこ病］、ｃｌｉｎｔｏｎｉｏｐｓｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｃｕｒｖｉｓｐｏｒａ（Ｈａｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イヌブナうどんこ病］、ｅｈｒｅｔｉａｅ（Ｋｅｉｓｓｌ．）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔａｉｗａｎｅｎｓｉｓ（Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［チシャノキうどんこ病］、ｆｒａｘｉｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｈｙｄｒａｎｇｅａｅ（Ｃｈｅｎ ＆ Ｇａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノリウツギうどんこ病］、ｋｕｓａｎｏｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｋｕｓａｎｏｉ［エノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ａｐｈａｎａｎｔｈｅｓ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムクノキうどんこ病］、ｋｕｓａｎｏｉ ｖａｒ．ｚｅｌｋｏｗａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ケヤキうどんこ病］、ｌａｔａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニシキウツギうどんこ病］、ｌｊｕｂａｒｓｋｉｉ ｖａｒ．ａｄｕｎｃｏｉｄｅｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオイタヤメイゲツうどんこ病］、ｌｊｕｂａｒｓｋｉｉ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｌｊｕｂａｒｓｋｉｉ［イロハモミジ・ヤマモミジうどんこ病］、ｍａｔｓｕｎａｍｉａｎａ（Ｎｏｍｕｒａ， Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ヤマハゼうどんこ病］、ｍｉｙａｂｅｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハンノキ類うどんこ病］、ｍｏｒｉ（Ｍｉｙａｋｅ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ｎｅｃａｔｏｒ［ブドウうどんこ病］、ｎｅｃａｔｏｒ Ｓｃｈｗｅｉｎｔｚ ｖａｒ．ａｍｐｅｌｏｐｓｉｄｉｓ（Ｐｅｃｋ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノブドウうどんこ病］、ｎｉｓｈｉｄａｎａ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アオギリうどんこ病］、ｏｌｅｏｓａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｏｌｅｏｓａ［シナノキうどんこ病］、ｏｌｅｏｓａ ｖａｒ．ｚｈｅｎｇｉｉ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバボダイジュうどんこ病］、ｐｉｃｒａｓｍａｅ（Ｈｏｍｍａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ニガキうどんこ病］、ｐｓｅｕｄｏｃａｒｐｉｎｉｃｏｌａ（Ｎｏｍｕｒａ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［サワシバうどんこ病］、ｓａｌｉｃｉ−ｇｒａｃｉｌｉｓｔｙｌａｅ Ｈｏｍｍａ［カワヤナギ・ネコヤナギうどんこ病］、ｓａｌｍｏｎｉｉ（Ｓｙｄｏｗ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トネリコ類うどんこ病］、ｓｃｈｉｚｏｐｈｒａｇｍａｔｉｓ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［イワガラミうどんこ病］、ｓｅｎｇｏｋｕｉ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ツルウメモドキうどんこ病］、ｓｅｐｔａｔａ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ＊［コナラ・カシワうどんこ病］、ｓｉｍｕｌａｎｓ（Ｓａｌｍｏｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｓｉｍｕｌａｎｓ［ノイバラうどんこ病］、ｓｉｍｕｌａｎｓ ｖａｒ．ｔａｎｄａｅ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ノイバラうどんこ病］、ｔｏｇａｓｈｉａｎａ ｖａｒ．ｒｉｇｉｄａ（Ｂｒａｕｎ ＆ Ｔａｎｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［オオバアサガラうどんこ病］、ｔｏｇａｓｈｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｔｏｇａｓｈｉａｎａ［ハクウンボクうどんこ病］、ｖｅｒｎｉｃｉｆｅｒａｅ（Ｈｅｎｎｉｎｇｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ウルシ・ヤマハゼ・ヌルデ・ケムリノキうどんこ病］、ｖｉｂｕｒｎｉｃｏｌａ Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガマズミ類うどんこ病］、Ｕｎｃｉｎｕｌａ ａｓｔｅｒｉｓ Ｓａｗａｄａ［ノコンギクうどんこ病］、ｃａｒｐｉｎｉ−ｃｏｒｄａｔａｅ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［サワシバうどんこ病］、ｍｉｙａｂｅｉ ｖａｒ．ｈｅｒｍａｐｈｒｏｄｉｔｉｃａ Ｔａｎｄａ ｅｔ Ｎｏｍｕｒａ［ヒメヤシャブシうどんこ病］、Ｇｏｌｏｖｉｎｏｍｙｃｅｓ：ａｄｅｎｏｐｈｏｒａ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［ツリガネニンジン・ソバナうどんこ病］、ａｒａｂｉｄｉｓ（Ｚｈｅｎｇ ＆ Ｃｈｅｎ）Ｇｅｌｊｕｔａ［エゾハタザオうどんこ病］、ａｒｔｅｍｉｓｉａｅ（Ｇｒｅｖｉｌｌｅ）Ｇｅｌｊｕｔａ［ヨモギ・オオヨモギ・オトコヨモギうどんこ病］、ｂｉｏｃｅｌｌａｔｕｓ（Ｅｈｒｅｎｂｅｒｇ）Ｇｅｌｊｕｔａ ｖａｒ．ｍｏｎａｒｄａｅ〔モナルダうどんこ病〕、ｂｒａｕｎｅｏｐｕｎｃｔａｔａ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ミゾホオズキうどんこ病］、ｃｉｃｈｏｒａｃｅａｒｕｍ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｇｅｌｊｕｔａ［ノコギリソウ類・アザミ類・ヒヨドリバナ類・ハンゴンソウ・ノゲシ・ホオズキなどナス科・キク科植物の多数のうどんこ病］、ｌａｐｏｒｔｅａｅ（Ｂｒａｕｎ）Ｇｅｌｊｕｔａ［ムカゴイラクサうどんこ病］、ｓｏｒｄｉｄｕｓ（Ｊｕｎｅｌｌ）Ｇｅｌｊｕｔａ［オオバコうどんこ病］、Ｅｒｙｓｉｐｈｅ ｃｉｃｈｏｒａｃｅａｒｕｍ ｄｅ Ｃａｎｄｏｌｌｅ ｖａｒ．ｌａｔｉｓｐｏｒａ Ｂｒａｕｎ［ブタクサうどんこ病］、Ｎｅｏｅｒｙｓｉｐｈｅ：ｃｕｍｍｉｎｓｉａｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ［モミジガサ・メタカラコウ・テバコモミジガサうどんこ病］、ｇａｌｅｏｐｓｉｄｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｂｒａｕｎ［オドリコソウ・ジャコウソウ・イヌゴマ・ヤマハッカ・ヒキオコシうどんこ病］、ｇｅｒａｎｉｉ（Ｎｏｍｕｒａ）Ｂｒａｕｎ［ゲンノショウコ・チシマフウロ・イチゲフウロ・エゾフウロ・ハクサンフウロうどんこ病］、Ｐｈｙｌｌａｃｔｉｎｉａ：ａｉｌａｎｔｈｉ（Ｇｏｌｏｖｉｎ ｅｔ Ｂｕｎｋｉｎａ）Ｙｕ［ニワウルシうどんこ病］、ａｌｎｉ Ｙｕ ＆ Ｈａｎ［ハンノキ類うどんこ病］、ａｍｐｅｌｏｐｓｉｄｉｓ Ｙｕ ＆ Ｌａｉ［サンカクヅルうどんこ病］、ｂｒｏｕｓｓｏｎｅｔｉａｅ−ｋａｅｍｐｆｅｒｉ Ｓａｗａｄａ［コウゾ裏うどんこ病］、ｆｒａｘｉｎｉ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｆｕｓｓ［トネリコ類裏うどんこ病・マサキうどんこ病］、ｇｕｔｔａｔａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ［ハシバミ類・マンサク・コブシ・マタタビ・裏うどんこ病・カンバ類・ハンノキ類・サワシバ・イヌシデ・フサザクラ・ニガキ・オオバアサガラ・ネジキ・ジャケツイバラうどんこ病］、ｊｕｇｌａｎｄｉｓ Ｔａｏ ＆ Ｑｉｎ［オニクルミ・サワグルミうどんこ病・シナサワグルミ裏うどんこ病］、ｋａｋｉｃｏｌａ Ｓａｗａｄａ［カキうどんこ病］、ｍａｇｎｏｌｉａｅ Ｙｕ ＆ Ｌａｉ［コブシ類裏うどんこ病］、ｍａｌｉ（Ｄｕｂｙ）Ｂｒａｕｎ［ナシ・セイヨウナシうどんこ病］、ｍｏｒｉｃｏｌａ（Ｈｅｎｎｉｎｇｓ）Ｈｏｍｍａ［クワ裏うどんこ病］、ｐｔｅｒｏｓｔｙｒａｃｉｓ Ｙｕ ＆ Ｌａｉ［オオバアサガラ裏うどんこ病］、ｒｏｂｏｒｉｓ（Ｇａｃｈｅｔ）Ｂｌｕｍｅｒ［クリ・アラカシ裏うどんこ病］、ｓａｌｍｏｎｉｉ Ｂｌｕｍｅｒ［キリうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｐｏｄｏｓｐｈａｅｒａ：ｃｅｒｃｉｄｉｐｈｙｌｌｉ Ｔａｎｄａ ＆ Ｎｏｍｕｒａ［カツラうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ａｕｃｕｐａｒｉａｅ（Ｅｒｉｋｓｓｏｎ）Ｂｒａｕｎ［ナナカマドうどんこ病］、ｃｌａｄｅｓｔｉｎａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）Ｌｅｖｅｉｌｌｅ ｖａｒ．ｃｌａｄｅｓｔｉｎａ［シモツケ類うどんこ病］、ｃｕｒｖｉｓｐｏｒａ Ｎｏｍｕｒａ［ナナカマドうどんこ病］、ｌｅｕｃｏｔｒｉｃｈａ（Ｅｌｌｉｓ ＆ Ｅｖｅｒｈａｒｔ）Ｓａｌｍｏｎ ｖａｒ．ｌｅｕｃｏｔｒｉｃｈａ［不完全世代：Ｏｉｄｉｕｍ ｆａｒｉｎｏｓｕｍ Ｃｏｏｋｅ、リンゴ・ズミうどんこ病］、ｌｏｎｇｉｓｅｔａ Ｓａｗａｄａ［ウワミズザクラ・ザイフリボクうどんこ病］、ｍｙｒｔｉｌｌｉｎａ（Ｓｃｈｂ．Ｆｒ）Ｋｕｎｚｅ ｖａｒ．ｍｙｒｔｉｌｌｉｎａ［クロウスゴうどんこ病］、ｓｐｉｒａｌｉｓ Ｍｉｙａｂｅ［ハルニレうどんこ病］、ｔｒｉｄａｃｔｙｌａ（Ｗａｌｌｒｏｔｈ）ｄｅ Ｂａｒｙ ｖａｒ．ｔｒｉｄａｃｔｙｌａ［不完全世代：Ｏｉｄｉｕｍ ｐａｓｓｅｒｉｎｉｉ Ｂｅｒｔｏｌｏｎｉ、モモ・スモモ・アンズ・ウメ・ユスラウメ・ニワウメ・サクラうどんこ病］、ｖｉｂｕｒｎｉ Ｂｒａｕｎ［ガマズミう類どんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｐ．［カマツカうどんこ病］、Ｐｏｄｏｓｐｈａｅｒａ ｓｅｃｔｉｏｎ Ｓｐｈａｅｒｏｔｈｅｃａ：ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ａｐｈａｎｉｓ［不完全世代：Ｏｉｄｉｕｍ ｒｕｂｏｒｕｍ Ｒａｂｅｎｈｏｒｓｔ、イチゴうどんこ病］、ａｐｈａｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ｖａｒ．ｈｙａｌｉｎａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［キジムシロうどんこ病］、ａｓｔｉｌｂｉｃｏｌａ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカショウマ・トリアシショウマうどんこ病］、ｂａｌｓａｍｉｎａｅ（Ｋａｒｉ ｅｘ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏｉｄｉｕｍ ｂａｌｓａｍｉｎａｅ Ｒａｊｄｅｒｋａｒ、ホウセンカ・キツリフネ・ツリフネソウうどんこ病］、ｃａｌｌｉｃａｒｐａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ムラサキシキブうどんこ病］、ｃａｒｄａｍｉｎｅｓ Ｎｏｍｕｒａ［タネツケバナうどんこ病］、ｃａｒｉｃａｅ−ｐａｐａｙａｅ（Ｔａｎｄａ ＆ Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｃａｒｉｃａｅ Ｎｏａｃｋ、パパイヤうどんこ病］、ｃｕｃｕｒｂｉｔａｅ（Ｊａｃｚｅｗｓｋｉｉ）Ｚｈａｏ［キュウリ・カボチャ・セイヨウカボチャ・ヒョウタンのうどんこ病］、ｄｉｃｌｉｐｔｅｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ハグロソウうどんこ病］、ｅｐｉｌｏｂｉｉ（Ｗａｌｌｒｏｔｈ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アカバナ類うどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｅｌｉｏｓｃｏｐｉａｅ（Ｔａｎｄａ ＆ Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［トウダイグサうどんこ病］、ｅｕｐｈｏｒｂｉａｅ−ｈｉｒｔａｅ（Ｂｒａｕｎ ＆ Ｓｏｍａｎｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［エノキグサうどんこ病］、ｆｅｒｒｕｇｉｎｅａ ｖａｒ．ａｌｂｉｆｌｏｒａｅ（Ｎｏｍｕｒａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［シロバナトウウチソウうどんこ病］、ｆｅｒｒｕｇｉｎｅａ（Ｓｃｈｌｅｃｈｔｅｎｄａｈｌ ｅｘ Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ ｖａｒ．ｆｅｒｒｕｇｉｎｅａ［ワレモコウうどんこ病］、ｆｕｇａｘ（Ｐｅｎｚｉｇ ＆ Ｓａｃｃ
ａｒｄ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ゲンノショウコうどんこ病］、ｆｕｓｃａ（Ｆｒｉｅｓ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［不完全世代：Ｏ．ｃｉｔｒｕｌｌｉ Ｙｅｎ ＆ Ｗａｎｇ、キュウリ・カボチャ・ユウガオ・トウガン・ゴボウ・フキ・コスモス・ヒマワリ・キクイモ・ペチュニア・オオキンケイギク・シオン・ダリア・リュウゼツサイ・ルドベキアうどんこ病］、ｈｉｂｉｓｃｉｃｏｌａ Ｚｈａｏ［フヨウうどんこ病］、ｉｎｔｅｒｍｅｄｉａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クサギうどんこ病］、ｍｏｒｓ−ｕｖａｅ（Ｓｃｈｗｅｉｎｉｔｚ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［スグリ類うどんこ病］、ｐａｎｎｏｓａ（Ｗａｌｌｒｏｔｈ：Ｆｒｉｅｓ）ｄｅ Ｂａｒｙ［不完全世代：Ｏ．ｌｅｕｃｏｃｏｎｉｕｍ Ｄｅｓｍａｚｉｅｒｅｓ， バラ・ハマナスうどんこ病］、ｐｈａｓｅｏｌｉ（Ｚｈａｏ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［アズキ・ヤブツルアズキうどんこ病］、ｐｓｅｕｄｏｆｕｓｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クワクサうどんこ病］、ｓｉｂｉｒｉｃａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［クガイソウ・コシオガマうどんこ病］、ｓｐａｒｓａ（Ｂｒａｕｎ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［ガガイモ・オオカモメズルうどんこ病］、ｓｐｉｒａｅａｅ（Ｓａｗａｄａ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［不完全世代：Ｏ．ｂｏｔｒｙｏｉｄｅｓ（Ｃｏｒｄａ）Ｃｅｓａｔｉ、シジミバナ・ユキヤナギ・オニシモツケうどんこ病］、ｓｔｅｐｈａｎａｎｄｒａｅ（Ｊａｃｚｅｗｓｋｉ）Ｂｒａｕｎ ＆ Ｔａｋａｍａｔｓｕ［コゴメウツギうどんこ病］、ｘａｎｔｈｉｉ（Ｃａｓｔａｇｎｅ）Ｂｒａｕｎ ＆ Ｓｈｉｓｈｋｏｆｆ［ウリ類・ヒマワリ・ヒャクニチソウ・フキ・ゴボウ・アメリカセンダングサ・キンセンカ・ベニバナ・アキノノゲシ・ヤマニガナ・ガーベラ・フヨウ・ナス・ヤブガラシ・エノキグサなど多数のうどんこ病］、Ｃｙｓｔｏｔｈｅｃａ：ｗｒｉｇｈｔｉｉ Ｂｅｒｋｅｌｅｙ ＆ Ｃｕｒｔｉｓ［カシ類紫かび病］、ｌａｎｅｓｔｒｉｓ（Ｈａｒｋｎｅｓｓ）Ｍｉｙａｂｅ［ナラ類紫かび病］、Ｔｙｐｈｕｌｏｃｈａｅｔａ：ｊａｐｏｎｉｃａ Ｉｔｏ ＆ Ｈａｒａ［ナラガシワ・ミズナラ・コナラ・トネリコうどんこ病］、Ｓａｗａｄａｅａ：ｂｉｃｏｒｎｉｓ（Ｗａｌｌｒｏｔｈ）Ｍｉｙａｂｅ［カエデ類うどんこ病］、ｐｏｌｙｆｉｄａ（Ｗｅｉ）Ｚｈｅｎｇ ＆ Ｃｈｅｎ ｖａｒ．ｊａｐｏｎｉｃａ Ｂｒａｕｎ ＆ Ｔａｎｄａ［モミジ類・カエデ類うどんこ病］、ｔｕｌａｓｎｅｉ（Ｆｕｃｋｅｌ）Ｈｏｍｍａ［カエデ類うどんこ病］、ｚｈｅｎｇｉｉ Ｎｏｍｕｒａ［エンコウカエデうどんこ病］、Ｂｌｕｍｅｒｉａ：ｇｒａｍｉｎｉｓ（ｄｅ Ｃａｎｄｏｌｌｅ）Ｓｐｅｅｒ［ムギ類うどんこ病］、Ｐｌｅｏｃｈａｅｔａ：ｓｈｉｒａｉａｎａ（Ｈｅｎｎｉｎｇｓ）Ｋｉｍｂｒｏｕｇｈ ＆ Ｋｏｒｆ［エノキ・ムクノキうどんこ病］、Ｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｓｅｕｄｏｐｅｒｏｎｏｓｐｏｒａ ｓｐｐ．、Ｐｌａｓｍｏｐａｒａ ｓｐｐ．、Ｂｒｅｍｉａ ｓｐｐ．、Ｂｒｅｍｉｅｌｌａ ｓｐｐ．、Ｃｒｏｎａｒｔｉｕｍ ｓｐｐ．、Ｍｅｌａｍｐｓｏｒａ ｓｐｐ．、Ｐｈａｋｏｐｓｏｒａ ｓｐｐ．、Ｕｒｅｄｉｎｏｐｓｉｓ ｓｐｐ．、Ｂｌａｓｔｏｓｐｏｒａ ｓｐｐ．、Ｇｙｍｎｏｓｐｏｒａｎｎｇｉｕｍ ｓｐｐ．、Ｐｕｃｃｉｎｉａ ｓｐｐ．、Ｕｒｏｍｙｃｅｓ ｓｐｐ．、Ａｅｃｉｄｉｕｍ ｓｐｐ．、Ｃａｅｍａ ｓｐｐ．、Ｐｅｒｉｄｅｒｍｉｕｍ ｓｐｐ．、Ｕｒｅｄｏ ｓｐｐ．、Ｐｈｒａｇｍｉｄｉｕｍ ｓｐｐ．、Ｓｔｅｒｅｏｓｔｒａｔｕｍ ｓｐｐ．、Ｔｒｉｃｈｏｌｏｍａ ｍａｔｓｕｔａｋｅ（Ｓ．Ｉｔｏ ｅｔ Ｉｍａｉ）Ｓｉｎｇ．、Ｔｕｂｅｒ ｓｐｐ．、Ｓｕｉｌｌｕｓ ｂｏｖｉｎｅｓ、Ｒｈｉｚｏｐｏｇｏｎ ｒｕｂｅｓｃｅｎｓ、および、ＶＡ菌根からなる群から選択される、請求項１に記載の方法。 The microorganism may be Erysiphe polygoni, Erysiphe pisi, Erysiphe paeoniae, Erysiphe cichoracerum, Erysiphe cichoracerum var. cichoracerum, sphaerotheca pannosa, sphaerotheca furiginea, sphaerotheca filipendulae, sphaerotheca balsamine bumamine, sphaerotheca fuscaulc. macrosporum, Oidium sp. , Sphaerotheca humuli, Erysiphe section Erysiphe: actinostematis Braun [Gokiru Miyamanigauri powdery mildew], aquilegiae de Candole var. aquilegiae [Audamaki powdery mildew], aquilegiae var. ranunculi (Greville) Zheng et Chen [Derhinium ranunculus, aconite, Akikara pine powdery mildew], braunii Nomura [Asahi goitou powdery mildew], betae (Vanha) Weltzien vice . corchori Tanda [Morohaya powdery mildew], celosiae Tanda [Nogateto powdery mildew], chloranthi (Golovin et Bunkina) Braun [Futarisuka powdery gourd disease] glycines [Yabamame, Natsufuji, Yabuhagi powdery mildew], glycines var lespedezae (Zheng et Braun) Braun et Zheng [Kehagigi Medhagi powdery mildew], gracilis Zhengvet gracilis [Aracasi, Shirakasi, Vulgaris mildew], gracilis var. longisima Nomura [red-eyed tsukubagasashi powdery mildew], heraclei de Candole [carrot, parsley powdery mildew], hommae Braun [naginata-jutsu-namiki-shon-powder-disease], huainensis Misohagi, Ezomisohagi powdery mildew], macleayae Zheng et Chen [takenigusa powdery mildew], malloti Chen et Gao [Akamegasiwa powdery mildew], mayorii Blumer var. japonica Braun et Nomura [thistle powdery mildew], mayorii Blumer var. mayorii [Noasami powdery mildew], otanii Nomura [Mungougoura powdery mildew], paeoniae Zheng et Chen [Peonies powdery mildew], pileae Braun [Mizudo powdery mildew], pile basil Powdery mildew], plectranthi Shin et La [Prunus serrata powdery mildew], polygoni de Candole [soba-tade powdery mildew], psilla Tanda et Nomura [Mulban conodium powdery mildew], rodgerisula Disease], stefaniae Tanda [Tamazakitsu rafuji powdery mildew] takamatsusui Nomur [Lotus powdery mildew], ulmariae Desmazeres [Pleurotus powdery mildew], Biciae-unijugae (Homma) Braun [Nantenhagi powdery mildew] & Takamatsu [Akebi powdery mildew], alphaitoides (Griffon et Maublanc) Braun & Takamatsu [Incomplete generation: Oidium quarcinum Thumen var. gemmiparam Ferraris, Kunugi Mizunara Sudazii powdery mildew], berberidicola Tai [Hobas holly guinea tenge powdery mildew], astragali de Candole Disease], berberidicola (F.L. Tai) Braun & Takamatsu [mushroom powdery mildew], berberidis (de Candole) Level var. asiatica (Braun) Braun & Takamasu [Hiroha snake noboraz barberry powdery mildew], berchemiae (Sawada) Braun & Takamatu [Black-bellied powdery mildew], blasti (Tai) Braun ) Braun & Takamatsu [American white powdery mildew], clethrae (Braun) Braun & Takamatsu [Rybu powdery mildew], coriarie (Nomura) Braun & Takamatsu [Dokutsugi] Disease], de tziae (Bunkina) Braun & Takamatsu [Utsugi powdery mildew], erlangshanensis (Yu) Braun & Takamatsu [Miyama nightingale Kagura powdery mildew], friestii Leveille var. dahurica (Braun) Braun & Takamatsu [black rose rose buckthorn powdery mildew], helwiniae (Sawada) Braun & Takamatsu [Hanaika powdery mildew], hommae (Braun) et Braun) Braun & Takamatsu [Okella powdery mildew], izuensis (Nomura) Braun & Takamatsu [Azalea powdery mildew], juglandis (Golovin) Braun & Takamasu disease Takamatsu [Popular powdery mildew] , Ligustri (Homma) Braun & Takamatsu [privet powdery mildew], lonicerae de Candolle var. lonicerae [honeysuckle powdery mildew], magnifica (Braun) Braun & Takamatsu [mokuren kobushi powdery mildew], magnoliae (Sawada) Braun & Takamasu mum ] Meliosmae (Tanda & Nomura) Braun & Takamatsu [Awabuki powdery mildew], menispermi Howe var. dahurica (Braun & Nomura) Braun & Takamatsu [Bats powdery mildew], menispermi Howe var. Sinomenii (Yu) Braun & Takamasu [Tsurafuji powdery mildew]; Braun & Takamatsu [Hiroha snake noboraz powdery mildew], nomurae Braun [Sawafutagi powdery mildew], orixae (Braun & Tanda) Braun & Takamatu ], Phyllant hi (Tanda & Braun) Braun & Takamatsu [Pinpodium powdery mildew], picrasmae (Sawada) Braun & Takamatsu [Nigaki powdery mildew], pseudorucera (Salmon) Braun & Braun Braun & Takamatsu var. pulchra (dogwood, wild bovine, dogwood powdery mildew), robinicola Braun & Takamatsu (harienju powdery mildew), russerii (Clinton) Braun & Takamatsu (skinny powdery mildew) ], Securinegae (Tai & Wei) Braun & Takamatsu [Popular powdery mildew], Shinanoensis (Tanda) Braun & Takamatsu [Pumbo powdery], viburni Duby's disease Honeybee powdery mildew], s Ubrichochoma (Braun) Braun & Takamatsu [Harienju powdery mildew], syringae-japonicae (Braun) Braun & Takamatu [Lilac powdery mildew], tiliae (Eliade) Braun disease. trifolii [red clover powdery mildew], vanbruntiana Gerard var. Sambuci-racemosae (Braun) Braun & Takamatsu [Elderberry powdery mildew]; , Yamada (Salmon) Braun & Takamatsu [Kemponashi powdery mildew], Microsphaera lonicerae-Romasissimae Tanda [Kouisukagura powdery mildew, aqua Sawada] brevista Tanda & Nomura [Chrysanthemum powdery mildew], sinensis Yu [Chestnut powdery mildew], Erysiphe section Uncinula: actinidiae (Miyabe ex Jaczewski) Braun & Tamura. actinidiae [Matatabi / sarnashi powdery mildew], actinidiae var. argutae (Nomura) Braun & Takamatsu [Sarnashi Matatabi powdery mildew], adunaca (Wallroth) Fries var. adunca [Bacco willow powdery mildew], adunca var. mandhurica (Miura) Braun & Takamatsu (willow powdery mildew), amanoi (Nomu)
ra) Braun & Takamatsu [Hymeya Shabushi powdery mildew], australiana (McAlpine) Braun & Takamatsu [Sarisuwa powdery mildew], betulina Braun & Takamasu bum ], Buckleyae (Nomura Tanda) Braun & Takamatsu [Tukubane powdery mildew], carpinicola (Hara) Braun & Takamatsu [pesticide powdery mildew], cedlelae (Tai) Braun & u [Elm powdery mildew , Clintoniopsis (Zheng & Chen) Braun & Takamatsu [Sterculiaceae powdery mildew], curvispora (Hara) Braun & Takamatsu [Fagus japonica powdery mildew], ehretiae (Keissl.) Braun & Takamatsu var. taiwanensis (Tanda) Braun & Takamatu [Chishanoki powdery mildew], fraxinicola Braun & Takamasu [green potato powdery], hydrangeae (Chen & Gao) Braun & Takao. . Kusanoi [Enoki powdery mildew], Kusanoi var. aphananthes (Braun) Braun & Takamatsu [Mukunoki powdery mildew], kusanoi var. zelkowae (Hennings) Braun & Takamatsu [keyberry powdery mildew], lata (Nomura & Tanda) Braun & Takamatsu [Nishikitsugi powdery mildew], ljubarskii var. aduncoides (Zheng & Chen) Braun & Takamatsu [Ooitama Meigetsu powdery mildew], ljubarskii Braun & Takamatsu var. ljubarskii [Bloem & Bun], Matsunamiana (Nomura, Tanda & Braun), Braun & Takamatsu [Yamahose powdery mildew], Miyabei [Mulberry powdery mildew], necker Schweintz var. necator [grape powdery mildew], necator Schweintz var. ampelopsis (Peck) Braun & Takamatsu [no grape powdery mildew], nisidana (Homma) Braun & Takamatsu [Aogiri powdery mildew], oleosa (Zheng & Chen) Braun & Takam. oleosa [linden powdery mildew], oleosa var. zhengii (Braun) Braun & Takamatsu [Popular powdery mildew], picrasmae (Homma) Braun & Takamatsu [Nigaki powdery mildew], pseudocarpinicola (Nomura). River willow, cat willow powdery mildew], salmonii (Sydow) Braun & Takamatu [ash powdery mildew], schizophragmatis (Tanda & Nomura) Braun & Takamasu Key powdery mildew], septata (Salmon) Braun & Takamatsu * [Quercus Kashiwa powdery mildew], simulans (Salmon) Braun & Takamatsu var. simulans [Neubara powdery mildew], simulans var. tandae (Braun) Braun & Takamatsu [Neubara powdery mildew], togashiana var. rigida (Braun & Tanda) Braun & Takamatsu [Plump powdery mildew], togashiana (Braun) Braun & Takamatsu var. Togashiana [Hakuunboku powdery mildew], verniciferae (Hennings) Braun & Takamatsu [Urushi yamahaze, Nurde-Kemlino powdery mildew], viburnicola Braun & Tamamatsuu carpini-cordatae Tanda et Nomura [Sawasiba powdery mildew], Miyabei var. Hermaphroditica Tanda et Nomura [Hymeya Shabushi powdery mildew], Golovinomyces: adenophora (Zheng & Chen) Geljuta [Turiganinjin sobana powdery mildew]・ Two Artemisia, Paramecium powdery mildew], biocellatus (Ehrenberg) Geljuta var. monadae (monalda powdery mildew), brauneopuncta (Braun) Geljuta [Mizohozuki powdery mildew], cichoracerum (de Candole) Geljuta [achilleas, thistles, hydrangeas Powdery mildew], laporteae (Braun) Geljuta [Plumpy powdery mildew], sordidus (Junell) Geljuta [Plumpy powdery mildew], Erysiphe cichoracerum de Candole var. latispora Braun [Ragweed powdery mildew], Neoerysiphe: cumminsiana (Braun) Braun [Momigasa, Metakarako, Tebakomomigusa powdery mildew], galeopsidis (de Candole) geranii (Nomura) Braun [Gennoshouko, Chishimafuuro, Ichigefuuro, Ezofuuro, Hakusanfu powdery mildew], Phyllactinia: ailanthii (Golovin et Bunkina) Yu [Niwaurushi] & Lai [Sankakudon powdery mildew], br oussonetiae-kaempferi Sawada [Urban powdery mildew], fraxini (de Candole) Fuss [Glass powdery mildew / Masaki powdery mildew], gutata (Wallroth: Fries) Powdery mildew, birch, alder, sawnhorn, Insode, Fusazakura, Nigaki, Oobaasagara, Neki, Jukutsuibara powdery mildew, juglandis Tao & Qin [Oyster powdery mildew], magnoliae Yu & Lai [Kobushi back powdery mildew], mary (Duby) Braun [Nasis, pear udon] Disease], moricola (Hennings) Homma [back mulberry powdery mildew], pterostyracis Yu & Lai [Obaasagara back powdery mildew], roboris (Gachet) Blumer [chestnut arakashi back powdery mildew], salmoni ], Posphaera section Podophaera: cercidylphyli Tanda & Nomura [Katsura powdery mildew], cladestina (Wallroth: Fries) Level bar. aucupariae (Eriksson) Braun [Nanakamado powdery mildew], cladestina (Wallroth: Fries) Levelile var. cladestina [Pleurotus mushroom powdery mildew], curvispoora Nomura [rowan powdery mildew], leukotricha (Ellis & Everhart) Salmon var. leucotrica [incomplete generation: Oidium farinosum Cooke, apple / sumi powdery mildew], longiseta Sawada [waiwazuzakura zifriboku powdery mildew], myrtillina (Schb. Fr) Kunze var. myrtillina [Crousgo powdery mildew], spiralis Miyabe [harunile powdery mildew], tridactyla (Wallilloth) de Barry var. tridactyla [incomplete generation: Oidium passeriniii Bertononi, peach, peach, apricot, ume, eusloume, niwaume, cherry powdery mildew], viburni Braun [burdock powdery mildew], Podosphaera sp. [Kamatsuka powdery mildew], Podophaera section Sphaerotheca: aphanis (Wallillo) Braun & Takamatsu var. aphanis [incomplete generation: Oidium ruborum Rabenhorst, strawberry powdery mildew], aphanis (Wallilloth) Braun var. hyalina (Braun) Braun & Takamatsu [Kojimushi white powdery mildew], astilbicola (Zhao) Braun & Takamatsu [Red-skinned mumps], balsaminoe (Kari ex Braun) Spinach, tsutsurifune, tsutsufunesou powdery mildew], callicarpae (Tanda & Nomura) Braun & Takamatsu [purple powdery mildew], cardamines Nomura [rae] : O. caricae Noack, papaya powdery mildew], cucurbitae (Jaczewskii) Zhao [cucumber, pumpkin, pumpkin, leopard powdery mildew], diclipterae (Nomura) Braun & Tamagrospo [Red-bellied powdery mildew], euphorbiae-helioscopiae (Tanda & Nomura) Braun & Takamasu [euphorbia powdery mildew], euphorbiae-hirtae (Braun & Soma). albiflorae (Nomura) Braun & Takamatsu [powdery mildew powdery mildew], ferruginea (Schletchendahl ex Fries) Braun & Takamatsu var. ferruginea [Walemoko powdery mildew], fugax (Penzig & Sacc
ard) Braun & Takamatsu [geno-shoko powdery mildew], fusca (Fries) Braun & Shishoff [Incomplete generation: O.D. citrulli Yen & Wang, cucumber, pumpkin, yugao, tougan, burdock, burdock, cosmos, sunflower, chrysanthemum, petunia, pachytia, zion, dahlia, ryuzesai, rudbeckia powdery mildew, hibiscicola Braun) Braun & Takamatsu [Wagashi powdery mildew], mors-uvae (Schweinitz) Braun & Takamatsu [currant powdery mildew], pannosa (Wallroth: Fries) de Bary [Incomplete generation: O. leucoconium Desmazeres, Rose Hamanus powdery mildew], phaseoli (Zhao) Braun & Takamatsu [Azuki, Abutsuki Azuki powdery mildew], pseudofusca (Braun) braun & taku・ Koshio Gama powdery mildew], sparsa (Braun) Braun & Takamatsu [Gaigamo okamuzuru powdery mildew], spiraea (Sawada) Braun & Takamatsu [Incomplete generation: O. botryoides (Corda) Cesati, rainbow trout, snowy canopy, powdery mildews Numerous powdery mildews such as calendula, safflower, akinonogeshi, yamanana, gerbera, buoy, eggplant, sorghum, enokigusa], Cytotheca: wrighti Berkeley & Curtis [purple mold fungus], lanestris H Disease], Typhlochaeta: japonica to & Hara [Quercus Aliena, Japanese oak, Quercus, ash powdery mildew], Sawadaea: bicornis (Wallroth) Miyabe [maple such powdery mildew], polyfida (Wei) Zheng & Chen var. japonica Braun & Tanda [momiji, maple powdery mildew], tulasnei (Fuckel) Homma (maple powdery mildew), zhengii Nomura [enkookae powdery mildew], Blumeria: praminisum Disease], Pleochaeta: shiraiana (Hennings) Kimbrough & Korf [Enoki Mukino powdery mildew], Peronospora spp. , Pseudoperonospora spp. Plasmopara spp. Bremia spp. Bremiella spp. Cronartium spp. Melampsora spp. Phakopsora spp. Uredinopsis spp. Blastosporia spp. Gymnosporangium spp. , Puccinia spp. Uromyces spp. , Aecidium spp. Caema spp. , Perdermium spp. , Uredo spp. Phragmidium spp. , Stereostratum spp. , Tricholoma matsutake (S. Ito et Imai) Sing. , Tuber spp. 2. The method of claim 1 , wherein the method is selected from the group consisting of: Silllus bovines, Rhizopogon rubescens, and VA mycorrhiza.
ａ）該微生物とその該共生者との該共生体を、乾燥する工程；および
ｂ）上記工程（ａ）の乾燥物を凍結する工程、
を包含する、方法であって、
ここで、該（ａ）乾燥工程が乾燥剤存在下において室温で３時間以上行われ、
該（ｂ）凍結する工程が、以下：
（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）液体窒素中で−１６５℃で凍結する工程
からなる群から選択され、
該微生物が、うどんこ病の病原菌、べと病のべと病菌、さび病のさび病菌、および、菌根菌からなる群から選択され、該共生者が植物である、方法。 A method for preserving a microorganism, wherein the microorganism is symbiotic with a symbiotic person, the method comprising:
a) a step of drying the symbiosis of the microorganism and the symbiotic person; and b) a step of freezing the dried product of the step (a).
A method comprising :
Here, the (a) drying step is performed at room temperature for 3 hours or more in the presence of a desiccant,
The step (b) freezing is as follows:
(I) pre-freezing at -70 ° C to -80 ° C and then freezing at -165 ° C in liquid nitrogen; and
(Ii) freezing at −165 ° C. in liquid nitrogen
Selected from the group consisting of
A method wherein the microorganism is selected from the group consisting of powdery mildew pathogen, downy mildew fungus, rust rust fungus, and mycorrhizal fungus, and the symbiosis is a plant .
ａ）微生物が感染した宿主生物から分生子を採集する工程；
ｂ）該分生子を乾燥する工程；および
ｃ）該分生子を凍結する工程、
を包含する、方法であって、
ここで、該（ｂ）乾燥工程が乾燥剤存在下において室温で３時間以上行われ、
該（ｃ）凍結する工程が、以下：
（ｉ） −７０℃〜−８０℃で予備凍結した後に、液体窒素中で−１６５℃で凍結する工程；および
（ｉｉ）液体窒素中で−１６５℃で凍結する工程
からなる群から選択され、
該微生物が、うどんこ病の病原菌、べと病のべと病菌、さび病のさび病菌、および、菌根菌からなる群から選択され、該宿主生物が、該病原菌に感染した植物である、方法。 A method for preserving microorganisms comprising:
a) collecting conidia from host organisms infected with microorganisms;
b) drying the conidia; and c) freezing the conidia.
A method comprising :
Here, the (b) drying step is performed at room temperature for 3 hours or more in the presence of a desiccant,
The step (c) freezing is as follows:
(I) pre-freezing at -70 ° C to -80 ° C and then freezing at -165 ° C in liquid nitrogen; and
(Ii) freezing at −165 ° C. in liquid nitrogen
Selected from the group consisting of
The microorganism is selected from the group consisting of powdery mildew pathogen, downy mildew fungus, rust rust fungus, and mycorrhizal fungus, and the host organism is a plant infected with the pathogen, Way .
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