JP6202672B2 - Tissue peeling prevention plate and culture unit - Google Patents

Description

  The present invention provides a tissue detachment prevention plate that is placed on a plurality of tissues arranged on the inner bottom surface of a culture vessel containing a culture solution, and prevents tissue detachment from the inner bottom surface of the culture vessel, and It relates to the culture unit used.

  Conventionally, as a tissue culturing technique, a technique is generally used in which a plurality of tissues are arranged at intervals on a culture container such as a petri dish, and culturing is performed in a culture solution placed in the culture container. In this technique, tissue culture is performed using the inner bottom surface of a culture vessel as a carrier (scaffold) for cell growth.

  However, in the above method, since the surface area of the culture vessel for attaching the cells is limited, in order to culture more cells, a large number of culture vessels or a large culture vessel is required, and the culture efficiency is increased. There was a problem of being bad.

  Therefore, a method is known in which a PET nonwoven fabric used for daily necessities or medical instruments is placed on a tissue in a culture container, and this PET nonwoven fabric is used as a carrier for culture (see Patent Document 1). ).

JP-A-11-56353

  However, in the conventional culture method using a PET nonwoven fabric, when observing a tissue and proliferating cells using a microscope, the PET nonwoven fabric is an obstacle to the field of view of the microscope, so that the tissue and proliferating cells cannot be observed well. There was a problem.

  In addition, when collecting tissues and proliferating cells, the tissue and proliferating cells are peeled off from the culture vessel and the PET nonwoven fabric by pouring a release agent. There was a problem that it was difficult to smoothly perform the peeling work with the agent. In addition, tissue or proliferating cells may be physically peeled off from a culture vessel or PET nonwoven fabric using a tool such as a spatula, but in this case as well, PET nonwoven fabric is prone to wrinkle and easily changes its shape. It is difficult to peel tissue and proliferating cells from PET nonwoven fabric. Furthermore, the tissue and proliferating cells are entangled with the fibers of the PET non-woven fabric, and the adhesion strength between the tissue and proliferating cells and the PET non-woven fabric is also a factor that makes it difficult to peel off the PET non-woven fabric.

  In addition, when exchanging the culture solution in the culture vessel, since the culture solution is infiltrated into the PET nonwoven fabric, it is difficult to remove the old culture solution infiltrated into the PET nonwoven fabric. There was a problem that it was difficult to carry out smoothly.

  In order to perform the culture well, it is necessary to bring the tissue into contact with the inner bottom surface of the culture vessel that serves as a scaffold for the tissue. There was a problem that the contact could not be maintained. In addition, since the PET nonwoven fabric, tissue, and proliferating cells float in the culture solution and the position is not constant, there is a problem that the tissue and proliferating cells cannot be observed well.

The main object of the present invention is to solve these problems, and to improve tissue culture efficiency, tissue detachment that enables good observation with a microscope, tissue recovery, and culture medium exchange. It is to provide a prevention plate and culture unit.
In addition, a secondary object of the present invention is to provide a tissue detachment prevention plate and a culture unit that maintain tissue contact with the inner bottom surface of the culture vessel and realize good culture.

The tissue peeling prevention plate of the present invention is placed on a plurality of tissues arranged on the inner bottom surface of a culture vessel containing a culture solution, and prevents tissue peeling from the inner bottom surface of the culture vessel. It is a tissue peeling prevention plate that also functions as a scaffold for culturing, and has a flat plate body, and a plurality of observation openings penetrating the front and back of the plate body, on the upper surface of the plate body, A stand engaging portion for attaching a stand for standing the tissue exfoliation prevention plate is provided so as to protrude upward from the upper surface of the plate main body, and the plate main body is elastic to the outside of the outer peripheral surface of the plate main body. deformably provided in contact with the culture vessel, by having a movement restricting portion that restricts movement of the upper and lower of the tissue peeling prevention plates, it solves the problems A.
The culture unit of the present invention is a culture unit including the tissue detachment prevention plate and the culture container, wherein the culture container is engaged with the tissue detachment prevention plate, The problem is solved by the provision of a restricting engagement portion that restricts the upward movement.

According to the first aspect of the present invention, it is a scaffold for culturing the tissue detachment prevention plate by having a flat plate main body and a plurality of observation openings penetrating the front and back of the plate main body. As a result, it is possible to maintain the state where the tissue is in contact with the inner bottom surface of the culture vessel as a scaffold for culturing, while improving the culture efficiency and suppressing the floating of the tissue by the plate body, and the observation opening A good microscope visual field can be ensured by the portion.
In addition, unlike the case of using a conventional PET nonwoven fabric, the tissue can be smoothly peeled off from the plate body using a device such as a peeling agent or a spatula, and the culture medium is infiltrated into the plate body. Therefore, the culture medium can be exchanged satisfactorily.
In addition, in order to keep the culture medium in a good state, it is necessary to include a certain amount of carbon dioxide, oxygen, etc. in the culture liquid, but carbon dioxide and oxygen taken from the liquid surface of the culture liquid through the observation opening. Can be smoothly distributed throughout the culture medium.
In addition, by providing a stand engaging portion for attaching the stand to the upper surface of the plate body, the tissue peeling prevention plate is placed on the work table so that the lower surface of the plate body faces upward by attaching the stand to the plate body. Since it can be made to stand, the operation | work which peels a structure | tissue from the lower surface of a plate main body can be performed easily. Similarly, various operations such as washing, sterilization, and drying of the tissue detachment prevention plate can be easily performed.

According to the second aspect of the present invention, the observation opening is formed with a maximum value of the opening width of 1.0 to 5.0 mm, so that the structure is secured while ensuring a good microscope field of view. It is possible to prevent the tissue from being lifted by preventing passage through the observation opening.
According to the invention of claim 3, the tissue peeling prevention plate has a specific gravity of 3.0 to 10.0 g / cm ³ , thereby avoiding the tissue from being crushed by the weight of the tissue peeling prevention plate. However, the state where the tissue is in contact with the inner bottom surface of the culture vessel can be reliably maintained.
According to the fourth aspect of the present invention, since the tissue peeling prevention plate is made of stainless steel, the stainless steel has a state in which the tissue contacts the inner bottom surface of the culture vessel while avoiding the tissue from being crushed. It has a specific gravity (7.6 to 8.1 g / cm ³ ) that can be maintained, does not migrate out substances that inhibit culture, has excellent durability and corrosion resistance, and is easy to sterilize. Culture can be realized.
According to the fifth aspect of the present invention, since the plate leg portion is provided on the lower surface of the plate body, the lower surface of the plate body and the inner bottom surface of the culture vessel can be separated at a predetermined interval. Regardless of the weight of the prevention plate, the tissue can be reliably prevented from being crushed between the lower surface of the plate body and the inner bottom surface of the culture vessel.
Moreover, the weight design of the tissue separation preventing plate is facilitated by avoiding the tissue from being crushed by the plate leg.
In addition, by providing the plate leg on the plate body side, it is not necessary to provide a portion such as a protrusion that performs the same function as the plate leg on the culture container side, so that it is possible to use a commonly used existing culture container. it can.
According to the first aspect of the present invention, the plate main body has the movement restricting portion that comes into contact with the culture container, thereby reliably moving the tissue peeling prevention plate up and down regardless of the weight of the tissue peeling prevention plate. In order to regulate, it is possible to maintain the state where the tissue is in contact with the inner bottom surface of the culture vessel while avoiding the tissue from being crushed by the tissue peeling prevention plate.
In addition, since the restrictions on the weight design of the tissue peeling prevention plate are reduced, a light material such as resin can be used as the material for the tissue peeling prevention plate, and the design focuses on other factors such as cost, sterility, and workability. It can be performed.
In addition, by providing the movement restricting portion on the plate body side, it is not necessary to provide a portion such as a protrusion that performs the same function as the above-described movement restricting portion on the culture container side, so that an existing culture container that has been widely used is used. be able to.
According to the sixth aspect of the present invention, the upper surface of the plate body is provided with the regulating spacer that contacts the lid member, thereby restricting the upward movement of the tissue peeling prevention plate regardless of the weight of the tissue peeling prevention plate. Thus, it is possible to reliably maintain the state where the tissue is in contact with the inner bottom surface of the culture container. In addition, since there are no restrictions on the weight design of the tissue detachment prevention plate, a light material such as resin can be used as the material for the tissue detachment prevention plate, and design that emphasizes other factors such as cost, sterility, and workability is important. It can be carried out. In addition, by providing the regulating spacer on the plate body side, it is not necessary to provide a portion that performs the same function as the regulating spacer described above on the lid member side, and thus it is possible to use a commonly used existing lid member.
According to the invention of claim 7 , the plurality of observation openings are arranged such that the minimum value of the distance between the observation openings adjacent to each other is equal to or less than the maximum value of the opening width. The growth state of the proliferating cells from the tissue can be reliably observed.
According to the eighth aspect of the present invention, by providing the culture vessel with the upward movement restricting engagement portion that restricts the upward movement of the tissue separation preventing plate, the tissue separation prevention can be performed regardless of the weight of the tissue separation preventing plate. It is possible to regulate the upward movement of the plate and reliably maintain the state where the tissue contacts the inner bottom surface of the culture vessel. In addition, since there are no restrictions on the weight design of the tissue detachment prevention plate, a light material such as resin can be used as the material for the tissue detachment prevention plate, and design that emphasizes other factors such as cost, sterility, and workability is important. It can be carried out.

The perspective view which shows the usage condition of the culture unit which is 1st Example. Sectional drawing which shows the usage condition of the culture unit of 1st Example. The perspective view which shows the usage condition of the culture unit of 2nd Example. The perspective view which shows the culture unit and stand which are 3rd Examples. The top view which shows the state which made the tissue peeling prevention plate which attached the stand in 3rd Example stand. Sectional drawing which shows the usage condition of the culture unit of 4th Example. Sectional drawing which shows the usage condition of the culture unit of 5th Example. Sectional drawing which shows the usage condition of the culture unit of 6th Example. Sectional drawing which shows the usage condition of the culture unit of 7th Example. Explanatory drawing which shows the usage condition of the culture unit of 8th Example seeing from upper direction. The graph which shows the experimental result of the structure | tissue peeling prevention plate of this invention.

  The culture unit of the present invention is for culturing a tissue such as an umbilical cord tissue, and is composed of a culture container, a lid member that covers the culture container, and a tissue peeling prevention plate disposed in the culture container. Yes.

  The culture container is a petri dish formed of a transparent synthetic resin such as acrylic, glass, or the like, and has a disc-shaped bottom plate and an outer peripheral wall that rises from an edge of the bottom plate. The lid member is a lid formed of a transparent synthetic resin such as acrylic or glass. The diameter of the culture vessel is 6 cm, 10 cm, 15 cm, or the like.

  The tissue exfoliation prevention plate has a plate-like plate body and is placed on a plurality of tissues arranged on the inner bottom surface of the culture container, while avoiding the tissue from being crushed, on the inner bottom surface of the culture container. It keeps the tissue in contact.

Here, in the first embodiment shown in FIG. 1 and FIG. 2, the second embodiment shown in FIG. 3, and the third embodiment shown in FIG. 4 and FIG. Is maintained while the tissue is in contact with the inner bottom surface of the culture vessel. In this case, in order to apply pressure to the tissue so that the tissue does not collapse, the tissue peeling prevention plate as a whole has a specific gravity of 3.0 to 10.0 g / cm ³ and 0.1 to 0.67 g / cm ^3. It is composed of a weight per unit area of ² . The weight per unit area is calculated by the area of the tissue peeling prevention plate not including the area of the observation opening. The opening ratio of the tissue detachment prevention plate is about 20 to 50%. In this case, as a material of the tissue detachment prevention plate, a substance that inhibits culture is not released, it is excellent in durability and corrosion resistance, is easy to sterilize (autoclave, etc.), and has an appropriate specific gravity (7.6 to A stainless steel having 8.1 g / cm ³ ) is preferred.

Further, in the fourth embodiment shown in FIG. 6 and the fifth embodiment shown in FIG. 7, the downward movement of the tissue separation preventing plate is regulated by utilizing contact and engagement with the culture vessel, and the tissue is pushed. While avoiding being crushed, the tissue is kept in contact with the inner bottom surface of the culture vessel by utilizing the weight of the tissue peeling prevention plate. In this case, in order to apply a pressure to the tissue so that the tissue does not collapse, the tissue peeling prevention plate as a whole has a specific gravity of 3.0 g / cm ³ or more and a unit area of 0.1 g / cm ² or more. Consists of weight.

In addition, in the sixth embodiment shown in FIG. 8 and the seventh embodiment shown in FIG. 9, the upward movement of the tissue separation preventing plate is regulated by using contact and engagement with the culture vessel and the lid member, Maintain the state where the tissue is in contact with the inner bottom surface of the culture vessel. In this case, in order to avoid the tissue from being crushed by the weight of the tissue peeling prevention plate, the tissue peeling prevention plate as a whole has a specific gravity of 10.0 g / cm ³ or less and 0.67 g / cm ² or less. The weight per unit area. Also, in this case, substances that inhibit the culture are not released, it is excellent in durability and corrosion resistance, is easy to sterilize (ethylene gas sterilization, etc.), and inexpensive resins such as polystyrene and ABS resin prevent tissue peeling. It can be used as a material for all or part of the plate.

  Further, in the eighth embodiment shown in FIG. 10, the movement of the tissue separation preventing plate is regulated by utilizing the contact with the culture vessel, and the tissue is prevented from being crushed while the tissue is kept inside the culture vessel. Maintain tissue in contact with the bottom. In this case, since there are few restrictions on the specific gravity and the like of the tissue peeling prevention plate, the above-described inexpensive resins such as polystyrene and ABS resin can be used as a material for the whole or a part of the tissue peeling prevention plate.

  On the other hand, the flat plate body is provided with an observation opening penetrating the front and back of the plate body. This observation opening ensures a good microscope field of view even when a tissue separation preventing plate is placed on a plurality of tissues.

  The specific form of the observation opening may be anything as long as it penetrates the front and back of the plate body. For example, as in the first embodiment shown in FIG. 1, holes formed in the plate body are used for observation. Examples of the case where it is used as an opening, and the case where it is used as an opening for observation as in the second embodiment shown in FIG.

  In addition, any shape or size of the observation opening can be used as long as it can prevent the tissue from lifting (that is, does not pass through the tissue) and can ensure a good microscope field of view. But you can. That is, the shape of the observation opening may be, for example, a circular shape as in the first embodiment shown in FIG. 1 or a rectangular shape as in the second embodiment shown in FIG. Further, regarding the size of the observation opening, regardless of the shape of the observation opening, the maximum value of the opening width (that is, the maximum value of the distance between two points on the outer periphery of the observation opening) is It is formed to be 1.0 to 5.0 mm, which is slightly smaller than the size of the tissue. Further, the plurality of observation openings are arranged so that the minimum value of the distance between the observation openings adjacent to each other is equal to or less than the maximum value of the opening width described above.

  Hereinafter, a plurality of embodiments of the culture unit of the present invention will be described with reference to the drawings.

  First, the culture unit according to the first embodiment of the present invention will be described with reference to FIG. 1 and FIG.

  As shown in FIGS. 1 and 2, the culture unit of the first embodiment includes a culture vessel 900, a lid member 910 (not shown in FIGS. 1 and 2), and prevention of tissue detachment disposed in the culture vessel 900. Plate 100.

  As shown in FIGS. 1 and 2, the tissue detachment prevention plate 100 has a disk-shaped plate body 110 made of stainless steel and a plurality of observation openings 120 formed by punching the plate body 110. ing. The diameter of the plate body 110 is determined according to the diameter of the culture vessel 900, such as 5.1 cm (when the diameter of the culture vessel 900 is 6.0 cm). Moreover, the thickness of the plate body 110 is about 0.5 to 1.5 mm.

  Next, a method for culturing the tissue T using the culture unit will be described below.

  First, a plurality of tissues T are arranged on the inner bottom surface of the culture vessel 900 together with physiological saline. Next, the tissue separation preventing plate 100 is installed on the plurality of tissues T so that the tissues T do not rise in the physiological saline. Next, the physiological saline is removed from the inside of the culture vessel 900 and dried appropriately, so that the tissue T is attached to the inner bottom surface of the culture vessel 900. Next, the culture solution C is added to the culture vessel 900. Finally, in a state where the culture solution C is put, it is stored for about 2 to 3 weeks, and the tissue T is cultured.

  The above-described method is an example of a tissue T culture method using a culture unit.

When the tissue peeling prevention plate 100 of the first embodiment configured as described above is used, as shown in FIG. 11, about 9.5 times as many cells as the case of using a conventional PET nonwoven fabric are used. I was able to get a number. In addition, when an ABS resin (specific gravity: about 1.05 g / cm ³ ) is used in the same manner as in this example, the number of cells is about 1.5 times that in the case of using a conventional PET nonwoven fabric. I was able to get it.

  Next, the culture unit according to the second embodiment of the present invention will be described with reference to FIG. Here, the second embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue peeling prevention plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 200th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the second embodiment, as shown in FIG. 3, the plate body 210 is made of a stainless steel ring-shaped frame member 211 and a stainless steel mesh member 212 fixed to the ring-shaped frame member 211. It consists of and. The mesh portion of the mesh member 212 functions as the observation opening 220.

  When the tissue peeling prevention plate 200 of the second embodiment configured as described above is used, as shown in FIG. 11, about 6.0 times as many cells as the case of using a conventional PET nonwoven fabric are used. I was able to get a number.

  Next, the culture unit which concerns on 3rd Example of this invention is demonstrated based on FIG. 4, FIG. Here, the third embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue separation preventing plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 300th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the third embodiment, as shown in FIG. 4, a stand attachment member 330 is fixed to the upper surface 310 a of the plate body 310 facing upward while being arranged in the culture vessel 900.

  As shown in FIG. 4, a pair of stand engaging portions 331 are formed in the stand mounting member 330, and the distal end portion of the stand 340 is inserted into and engaged with the pair of stand engaging portions 331. Thus, as shown in FIG. 5, the stand 340 is attached to the tissue separation preventing plate 300.

In addition, the specific aspect of the engaging part 331 for stands is not limited above, As long as it can engage with the stand 340 and the stand 340 can be stably attached to the tissue separation preventing plate 300. Anything is acceptable. For example, it may be provided integrally with the stand engage portion 331 to the plate body 310, also but it may also be configured to be detachably attached to the stand engage portion 331 to the plate body 310.

  Next, the culture unit according to the fourth embodiment of the present invention will be described with reference to FIG. Here, the fourth embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue peeling prevention plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 400th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the fourth embodiment, as shown in FIG. 6, a plate leg 450 is fixed to the lower surface 410 b of the plate body 410 facing downward while being arranged in the culture vessel 900. In order to suppress the inclination of the plate body 410, the plate legs 450 are preferably provided in at least three places, or formed in a ring shape in the vicinity of the edge of the plate body 410.

  The specific aspect of the plate leg 450 is not limited to the above, and the lower surface 410b of the plate body 410 and the inner bottom surface of the culture vessel 900 are spaced apart at a predetermined interval to prevent the tissue T from being crushed. Anything can be used. For example, the plate leg 450 may be provided integrally with the plate body 410. Moreover, you may provide the leg part which performs the function similar to the plate leg part 450 in the culture container 900 side.

  Next, the culture unit according to the fifth embodiment of the present invention will be described with reference to FIG. Here, the fifth embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue peeling prevention plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 500th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the fifth embodiment, as shown in FIG. 7, a protrusion that engages with the plate body 510 on the inner peripheral surface of the outer peripheral wall of the culture vessel 900 and regulates the downward movement of the tissue separation preventing plate 500. A downward movement restriction engaging portion 920 is provided. The downward movement restricting engagement portions 920 are provided at least at three locations on the inner peripheral surface of the outer peripheral wall of the culture vessel 900 or are provided in a ring shape.

  Next, the culture unit according to the sixth embodiment of the present invention will be described with reference to FIG. Here, the sixth embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue peeling prevention plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 600th series, the description of the configuration other than the difference will be omitted.

  In the culture unit according to the sixth embodiment, as shown in FIG. 8, the inner surface of the outer peripheral wall of the culture vessel 900 is engaged with the plate body 610 and elastically restricts the upward movement of the tissue detachment prevention plate 600. A deformable projecting upward movement restricting engagement portion 930 is provided. The upward movement restricting / engaging portion 930 is provided in at least one place on the inner peripheral surface of the outer peripheral wall of the culture vessel 900 or in a ring shape.

  Next, a culture unit according to the seventh embodiment of the present invention will be described with reference to FIG. Here, the seventh embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue separation preventing plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 700th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the seventh embodiment, as shown in FIG. 9, the upper surface 710 a of the plate body 710 that faces the upper side in the state of being placed in the culture vessel 900 is brought into contact with the lid member 910 and the tissue peeling prevention plate 700 A regulating spacer 760 that regulates the upward movement of is fixed. The restriction spacer 760 also functions as a handle for the tissue peeling prevention plate 700.

  The lid member 910 is designed to have a weight that does not move upward due to the force from the tissue detachment prevention plate 700, or the outer peripheral surface of the outer peripheral wall of the lid member 910 and the outer peripheral surface of the outer peripheral wall of the culture vessel 900. Further, by providing engaging portions (not shown) that engage with each other, the lid member 910 is configured not to move upward even when a force is received from the tissue separation preventing plate 700.

  In addition, you may provide the leg part which performs the function similar to the control spacer 760 in the culture container 900 side.

  Next, a culture unit according to the eighth embodiment of the present invention will be described with reference to FIG. Here, the eighth embodiment is exactly the same as the first embodiment described above except for a partial configuration. Therefore, by replacing the reference numerals of the 100th series of the tissue separation preventing plate shown in the specification and drawings relating to the first embodiment with the reference numerals of the 800th series, the description of the configuration other than the difference will be omitted.

  In the culture unit of the eighth embodiment, as shown in FIG. 10, a plurality of elastically deformable protruding movement restricting portions 870 protruding outward are formed on the outer peripheral surface of the plate body 810. Accordingly, the tissue peeling prevention plate 800 is restricted from moving up and down with respect to the culture vessel 900. The dotted line shown in FIG. 10 represents the outer peripheral wall of the culture vessel 900.

  In addition, the specific aspect of the movement control part 870 is not limited to the above, What kind of thing may be used as long as it contacts the culture container 900 and controls the movement of the tissue separation preventing plate 800 up and down. For example, the movement restricting portion 870 may be provided integrally with the plate body 810 or may be provided separately. Further, the movement restricting portion 870 may be provided in a ring shape outside the outer peripheral surface of the plate body 810, or may be provided separately in a plurality as shown in FIG. Moreover, you may provide the site | part which performs the function similar to the movement control part 870 in the culture container 900 side.

  Note that the culture unit may be configured by arbitrarily combining the configurations of the first to eighth embodiments described above. In the above description, the culture vessel is described as a petri dish having a disc-shaped bottom plate and an outer peripheral wall. However, the culture vessel may have any specific form such as a rectangular bottom plate. In the above-described embodiments, one tissue peeling prevention plate is installed for one culture container. However, a plurality of tissue peeling prevention plates may be installed for one culture container. Good. Further, a handle for taking out may be provided on the tissue peeling prevention plate. Further, the plate body may be composed of a single member or a plurality of members. In the above description, the tissue is an umbilical cord tissue, but the specific type of tissue is not limited to this. Further, the shape of the plate body may be any shape such as a disk shape or a rectangular plate shape. The shape of the plate body is desirably a shape that matches the shape of the outer peripheral wall of the culture vessel.

100, 200, 300, 400, 500, 600, 700, 800 ... Tissue peeling prevention plate 110, 210, 310, 410, 510, 610, 710, 810 ... Plate body 120, 220, 320, 420, 520, 620, 720, 820... Observation opening 211... Frame member 212... Net member 310a, 710a... Plate plate upper surface 410b... Plate body lower surface 330. Mounting member 331 ... Stand engaging part 340 ... Stand 450 ... Plate leg part 760 ... Restricting spacer 870 ... Movement restricting part 900 ... Culture vessel 910 ... Lid member 920・ ・ ・ Downward movement restriction engagement portion 930 ・ ・ ・ Upward movement restriction engagement portion T ・ ・ ・ Tissue C ・ ・ ・Nutrient solution

Claims (8)

A tissue that is placed on a plurality of tissues disposed on the inner bottom surface of a culture vessel containing a culture solution, prevents the tissue from peeling off from the inner bottom surface of the culture vessel, and also functions as a scaffold for tissue culture An anti-peeling plate,
A flat plate body, and a plurality of observation openings penetrating the front and back of the plate body,
On the upper surface of the plate body, a stand engaging portion for attaching a stand for standing the tissue peeling prevention plate is provided so as to protrude upward from the upper surface of the plate body.
The plate main body is provided on the outside of the outer peripheral surface of the plate main body so as to be elastically deformable, and has a movement restricting portion that comes into contact with the culture vessel and restricts the vertical movement of the tissue separation preventing plate. Tissue peeling prevention plate.   The tissue peeling prevention plate according to claim 1, wherein the observation opening is formed with a maximum opening width of 1.0 to 5.0 mm through which the tissue cannot pass. The tissue peeling prevention plate according to claim 1 or 2, wherein the tissue peeling prevention plate has a specific gravity of 3.0 to 10.0 g / cm ³ . The tissue ablation preventing plate, tissue peeling prevention plate according to claim 3, characterized in that it is constructed from stainless steel.   5. The plate leg for separating the lower surface of the plate main body from the inner bottom surface of the culture vessel at a predetermined interval is provided on the lower surface of the plate main body. Tissue peeling prevention plate in any one.   The upper surface of the plate main body is provided with a regulating spacer that contacts a lid member that covers the culture vessel and regulates the upward movement of the tissue detachment preventing plate. Item 6. The tissue detachment prevention plate according to any one of Items 5.   The plurality of observation openings are arranged such that a minimum value of distances between observation openings adjacent to each other is equal to or less than a maximum value of the opening width. The tissue detachment prevention plate according to any one of the above. A culture unit comprising the tissue detachment prevention plate according to any one of claims 1 to 7, and the culture container,
The culture unit is provided with an upward movement restricting engagement portion that engages with the tissue peeling prevention plate and restricts the upward movement of the tissue peeling prevention plate.

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