JP6279499B2 - Skin moisturizer - Google Patents

Description

  The present invention relates to a skin moisturizer, and more particularly to a skin moisturizer using an oyster (oyster) extract.

  Oysters contain abundant nutrients such as high-quality proteins, taurine, minerals and vitamins, and conventionally, pharmaceutical compositions using this oyster extract have been proposed.

  For example, a function-enhanced supplement (see Patent Document 1) containing an antioxidant substance containing at least vitamin C25 to 35 W% and an antioxidant enhancer substance containing at least 1 to 3 W% oyster meat extract, eggshell membrane or its water A food composition for skin improvement containing a decomposed product and an oyster extract (see Patent Document 2) has been proposed.

  In addition, a pharmaceutical composition for preventing or treating gastric ulcer containing oyster extract as an active ingredient (see Patent Document 3), or a pharmaceutical composition for preventing or treating male infertility containing oyster extract as an active ingredient ( Patent Document 4) has been proposed.

  However, in the compositions shown in Patent Documents 1 and 2, the oyster extract is only used as an auxiliary component, and does not pursue the efficacy of the oyster extract alone. Moreover, the composition of patent document 3 and 4 is related with specific effects, such as a stomach ulcer and male infertility.

JP 2007-37416 A JP 2003-245055 A JP-A-11-322617 JP-A-11-322626

  The subject of this invention is providing the skin moisturizer which has the protection effect with respect to an ultraviolet-ray.

  As a result of intensive research and research on the efficacy of oysters, the inventors of the present invention have found that the extract obtained from oyster meat (oyster meat) recovers horny water that has been reduced by irradiation with ultraviolet rays (ultraviolet B wave (UVB)). It has been found that there is a moisture recovery promoting action, and the present invention has been completed. Furthermore, it has been found that by containing collagen together with oyster extract, this keratin moisture recovery promoting action can be improved to the same level or more.

That is, the present invention relates to a skin moisturizer characterized by containing oyster extract as an active ingredient.
The skin moisturizing agent of the present invention moisturizes the skin due to the keratin moisture recovery promoting action of recovering keratin moisture reduced by ultraviolet irradiation, and is preferably an oral preparation.
The oyster extract in the skin moisturizing agent of the present invention is preferably an extract concentrate obtained by extracting oyster meat with water, or an enzyme-decomposed concentrate obtained by enzymatically degrading oyster meat.
The skin moisturizer of the present invention preferably further contains collagen.

  The skin moisturizing agent of the present invention can moisturize the skin by recovering keratinous water that has been reduced by ultraviolet irradiation.

It is a figure which shows the measurement result (actual value) of the amount of stratum corneum in the female hairless mouse to which the skin moisturizing agent of the present invention is applied from before UVB irradiation to 5 days after UVB irradiation. It is a figure which shows the measurement result (change value) of the amount of stratum corneum in the female hairless mouse to which the skin moisturizer of the present invention is applied from before UVB irradiation to 5 days after UVB irradiation. It is a figure which shows the measurement result (change value) of the amount of keratin moisture to the 28th day of the male hairless mouse (low protein diet load) to which the skin moisturizer of this invention is applied. It is a figure which shows the measurement result (change value) of the amount of keratin moisture after UVB irradiation of the male hairless mouse (low protein diet load) to which the skin moisturizer of this invention is applied. It is a figure which shows the measurement result of the skin thickness after 28-day progress of the male hairless mouse (low protein diet load) to which the skin moisturizer of this invention is applied. The normal group, the control group, the oyster extract 10% group, the oyster extract 10% + collagen 1% group, and the collagen 1% group are shown in order from the left side. The measurement result of the amount of hyaluronic acid (HA) in the skin after 28 days of male hairless mice (low protein diet load) to which the skin moisturizer of the present invention is applied is shown. The normal group, the control group, the oyster extract 5% group, the oyster extract 10% group, the oyster extract 5% + collagen 1% group, the oyster extract 10% + collagen 1% group, and the collagen 1% group are shown in order from the left side. The measurement result of the amount of mRNA in skin of the male hairless mouse (low protein diet load) to which the skin moisturizing agent of the present invention is applied after 28 days is shown. The normal group, the control group, the oyster extract 5% group, the oyster extract 10% group, the oyster extract 5% + collagen 1% group, the oyster extract 10% + collagen 1% group, and the collagen 1% group are shown in order from the left side.

  The skin moisturizer of the present invention is not particularly limited as long as it contains oyster extract as an active ingredient, and the skin moisturizer of the present invention recovers keratinous moisture that has been reduced by ultraviolet (UVB) irradiation. The skin can be moisturized by a moisture recovery promoting action (a stratum corneum moisture reduction inhibitory action that suppresses the reduction of the stratum corneum moisture caused by ultraviolet irradiation). In addition, the skin moisturizer of the present invention functions as a hyaluronic acid synthesis promoter because it has an action of promoting the synthesis of hyaluronic acid in the skin.

  The oyster (oyster) used as a raw material for the oyster extract as an active ingredient of the skin moisturizing agent of the present invention is not particularly limited as long as it is an oyster belonging to the family Sphagnumaceae, and the oyster belonging to the genus Magaki or the oyster belonging to the genus Itabaki Can be illustrated.

  Examples of the oyster extract of the present invention include those obtained by extracting these oyster bodies (oyster meat) with a solvent, and those obtained by hydrolyzing oyster meat with an enzyme. Examples of the extraction solvent include water and alcohol. Moreover, as a hydrolase, the kind will not be ask | required if it is an enzyme which hydrolyzes a foodstuff material (protein).

  These extraction treatments and enzyme treatments can be performed by heating and / or pressurization as necessary. Moreover, in order to obtain more active ingredients, it is preferable to pulverize oyster meat in advance before extraction treatment and enzyme treatment.

  For example, crushed oyster meat is extracted with water at about 40 to 90 ° C. for about 1 to 5 hours, concentrated after removing oyster meat residue, and subjected to spray drying, lyophilization, etc. as necessary, in liquid or powder form An oyster extract (extracted concentrate) can be obtained.

  Moreover, after pulverized oyster meat is hydrolyzed by acting an enzyme in water at about 40 to 80 ° C. to deactivate the enzyme, unnecessary substances such as oyster meat residue are removed, concentrated, and if necessary. Spray-drying, freeze-drying, etc. can be performed to obtain a liquid or powdery oyster extract (extracted concentrate).

  The oyster extract of the present invention may be the extract itself, but is preferably a liquid, semi-solid or solid concentrate obtained by concentrating the extract. Specifically, as the oyster extract, a commercially available product containing the oyster extract can be used, for example, “Marugoto concentrated oyster extract powder” manufactured by Toyo Shinyaku Co., Ltd., which is an enzyme-treated product of oyster meat.

  The skin moisturizer of the present invention preferably further contains collagen. By containing collagen together with oyster extract, the keratin moisture recovery promoting action can be improved to the same level or more. Although it does not restrict | limit especially as content of collagen in the skin moisturizer of this invention, For example, it is preferable to make it contain 0.1-50 mass% in a moisturizer, and make it contain 1-30 mass%. It is more preferable.

  The collagen contained in the skin moisturizing agent of the present invention may be animal-derived collagen or synthetic collagen. In addition to collagen protein, collagen peptide obtained by hydrolyzing collagen protein, collagen molecule Is treated with a protease, and atelocollagen from which the telopeptide moiety has been removed is included. As animal-derived collagen, collagen derived from fish is particularly preferable.

  The average molecular weight (weight average molecular weight) of the collagen of the present invention is not particularly limited, but is preferably 500 to 100,000, more preferably 1,000 to 50,000, and more preferably 2,000 to 15,000. It is particularly preferable that 3000 to 10,000 is most preferable.

Skin moisturizer of the present invention can be used as an oral agent can be a pharmaceutical or the like. The oral preparation is not particularly limited as long as it contains oyster extract and is taken orally .

  The skin moisturizer of the present invention contains oyster extract and is not particularly limited as long as it can be distinguished from other products as a product in terms of moisturizing skin or promoting keratin moisture recovery, For example, the display of the fact that any of the main body, packaging, instructions, and promotional material of the product according to the present invention has a function of skin moisturizing action and keratin moisture recovery promoting action is included in the scope of the present invention.

  When the skin moisturizer of the present invention is used as an oral preparation, it can be produced by a known preparation method by adding a base or carrier that is acceptable for oral use, if necessary. For example, tablet, granule, capsule, granule, rod, plate, block, solid, round, liquid, paste, cream, caplet, gel, It can be in the form of a chewable or stick-like agent. Among these, the form of a tablet, a granule, and a capsule is especially preferable. In the present invention, a granule is a concept that includes not only granules formed by solidifying powder by granulation or the like, but also powders that are not formed.

  Moreover, there is no restriction | limiting in particular in mix | blending another component with the skin moisturizer as an oral preparation of this invention, You may mix | blend components other than an oyster extract as needed. Examples of components other than oyster extract include vitamins such as ascorbic acid (vitamin C), folic acid and β-carotene; minerals such as calcium, magnesium, phosphorus and iron; sulfur-containing compounds contained in taurine and garlic; hesperidin And flavonoids such as quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils;

  The content of the oyster extract in the skin moisturizer of the present invention may be appropriately contained within the range where the effect is exerted, for example, in the skin moisturizer as the pharmaceutical of the present invention, in terms of the dry mass of the oyster extract, The oyster extract is preferably contained in an amount of 0.01 to 70% by mass, more preferably 0.1 to 50% by mass, and further preferably 0.5 to 30% by mass. .

  The intake amount of the skin moisturizing agent of the present invention is not particularly limited, but from the viewpoint of exerting the effects of the present invention more remarkably, the intake amount of oyster extract per day is 0.1 mg / day or more. It is preferable to take 0.5 mg / day or more, and it is more preferable to take 1 mg / day or more. The skin moisturizing agent of the present invention can be stored in one container or divided into a plurality of containers, for example, as one day so that the daily intake becomes the above intake.

  The weight ratio of oyster extract and collagen in the skin moisturizer of the present invention may be appropriately contained within the range where the effect is exerted, but preferably contains 0.01 parts by weight or more of collagen with respect to 1 part by weight of oyster extract. , 0.05 part by weight or more is more preferable, and 0.1 part by weight or more is most preferable. Further, the upper limit of the collagen content with respect to 1 part by weight of the oyster extract is not particularly limited, but for example, 100000 parts by weight or less, 50000 parts by weight or less, 30000 parts by weight or less, 10000 parts by weight or less, 5000 parts by weight or less. 1000 parts by weight or less, 500 parts by weight or less, 100 parts by weight or less, 50 parts by weight or less, 10 parts by weight or less.

Hereinafter, the present invention will be described based on examples.
[Example 1]
Seven-week-old female hairless mice (HR-1) were acclimated for 5 days and then divided into 5 groups so that the body weight values were almost uniform. As the UVB irradiation + oyster extract intake group, oyster extract 1%, 3% and 5% groups were set. A control group was set as a control for UVB irradiation.

  The test feed was mixed with MF powder feed (Oriental Yeast Industry Co., Ltd.) using a mortar and pestle so that the “whole concentrated concentrated persimmon extract powder” (manufactured by Toyo Shinyaku Co., Ltd.) was uniform. The blending amounts are as follows.

The test feed was ingested from 7 days before UVB irradiation (day 0) to 5 days after UVB irradiation (day 12). As a rule, the method of feeding was free intake. On the seventh day from the start of the test, UVB 150 mJ / cm ² was irradiated once.

Specifically, UVB irradiation was performed by irradiating the back of the mouse with UVB in a UV low pressure mercury lamp TL / 12 series UV-B region (TL20W / 12RS; Philips). The head was covered with aluminum foil so that UVB did not hit the head of the mouse, and UVB was irradiated at 150 mJ / cm ² . At the time of UVB irradiation, the mouse was anesthetized by intraperitoneal administration (5 mL / kg) of sodium thiopental (10 mg / mL).

  The moisture content in the back skin was measured every day from the UVB irradiation day (7th day) to 5 days after UVB irradiation (12th day).

  Specifically, the measurement of the amount of horny water is performed using a moisture checker (MY-808S: SCARA Co., Ltd.) at the center of the back skin of the mouse from the UVB irradiation day (7th day) to 5 days after the UVB irradiation (12th day). went. The UVB irradiation date was measured before irradiation. The measurement was carried out five times, and a numerical value obtained by averaging three values excluding the minimum and maximum values was taken as the measurement value.

  FIG. 1 shows a measurement result (actual value) of keratin water content from before irradiation on the day of UVB irradiation (day 7) to five days after UVB irradiation (day 12), and FIG. Change value).

  As shown in FIG.1 and FIG.2, compared with the control group, in any group of the present invention, the decrease in the amount of stratum corneum due to UVB irradiation was suppressed (the amount of stratum corneum recovered).

[Example 2]
Seven-week-old male hairless mice (HR-1) were acclimated for 5 days or more and then grouped based on TEWL and body weight (priority order: TEWL> body weight). As a subject group of the present invention, a oyster extract 10% group, a oyster extract 10% + collagen 1% group were set, and a test feed (low protein feed) corresponding to each group was freely ingested until the end of the test. In addition, a normal group for ingesting a normal feed, a control group for ingesting a low protein diet, and a collagen 1% group to which only collagen was added were set (hereinafter, these may also be referred to as a comparison group). The composition of the feed used is as follows.

  For the subject group and the comparative group of the present invention, on the test feed intake day 0, 7, 14, 21, 28, and after UVB irradiation (irradiation started on day 28, 3 days from day 30 to 32), The keratin water content was measured in the same manner as in Example 1. In addition, the mice on the 28th day of the test feed intake were laparotomized immediately after cervical dislocation, the back skin tissue was collected, and the epidermis thickness was measured. Specifically, the length of 15 places per individual was measured by image analysis to determine the thickness of the epidermis. 3 and 4 show the measurement results of the keratin water content, and FIG. 5 shows the skin thickness results.

  As shown in FIG. 3 and FIG. 4, compared with the control group, the group of the present invention was able to suppress the decrease in the amount of horny water due to low protein diet load and UVB irradiation (recovered the amount of horny water). Moreover, as shown in FIG. 5, compared with the control group, the group of the present invention significantly increased the epidermis thickness and was able to suppress thinning of the keratinous epidermis. In particular, a higher effect was obtained by taking collagen in combination with oyster extract.

[Example 3]
As in Example 2, the amount of hyaluronic acid (HA) in the skin and the amount of mRNA in the skin were measured on the 28th day of the day after ingesting the test feed. In this example, the oyster extract 5% group and the oyster extract 5% + collagen 1% group were also added for the experiment. In addition, adjustment of increase / decrease in the oyster extract and collagen in the test feed was performed by adjusting the amount of β corn starch.

  The amount of hyaluronic acid (HA) in the skin was measured according to the procedure of a commercially available kit (Hyaluronan Quantikine ELISA Kit: R & D SYSTEMS) after exfoliating the skin, cutting out the back skin with a punch, extracting HA. In addition, the amount of mRNA in the skin was measured using the obtained cDNA after skin peeling, by cutting out the back skin with a punch, extracting RNA, synthesizing cDNA. Specifically, measurements were performed for Has2 and Has3. FIG. 6 shows the measurement result of the amount of hyaluronic acid (HA) in the skin, and FIG. 7 shows the result of the amount of mRNA in the skin.

  As shown in FIGS. 6 and 7, in the group of the present invention, the amount of hyaluronic acid (HA) in the skin and the expression of the Has gene in the skin increased. The Has gene is a gene involved in the synthesis of hyaluronic acid. Therefore, the present invention is also effective as a hyaluronic acid synthesis accelerator. Moreover, it is guessed that the skin moisturizer of this invention acts on the amount of HA and expression of Has gene in skin, and is exhibiting the skin protection effect.

[Example 4] (Production of soft capsule)
The content liquid having the composition shown in Table 1 below was prepared and filled into a capsule film prepared by the composition shown in Table 2 to obtain soft capsules (the content liquid content per one capsule was 500 mg). Encapsulation was performed by casting a capsule coating solution to form a film, filling the content solution inside, heat-sealing, and drying the formed soft capsule. As the oyster extract, “whole concentrated concentrated oyster extract powder” (manufactured by Toyo Shinyaku Co., Ltd.) was used.
It is expected that the skin moisturizing effect can be obtained by ingesting 1 capsule per day (ingestion amount of oyster extract per day 1 mg).

[Example 5] (Production of granules)
Each component was mixed in the ratio shown in Table 5 below, and granulated by fluidized bed granulation. The obtained granulated product was sieved with a 30-mesh sieve to obtain granules.
It is expected that the moisturizing effect of the skin can be obtained by ingesting 1000 mg of this granule per day (ingestion amount of oyster extract per day 1 mg).

[Example 6] (Manufacture of tablets)
Each component was mixed in the ratio shown in Table 6 below, and tableting was performed with a tableting machine (300 mg per tablet).
It is expected that the moisturizing effect of the skin can be obtained by making this tablet 1 tablet per day (intake amount of oyster extract per day 0.1 mg).

Skin moisturizer of the present invention has the effect of moisturizing the skin enhances the stratum corneum water content, since it can be used as an oral agent, useful in industry is high.

Claims (3)

An oral skin moisturizer comprising oyster extract as an active ingredient. The oral skin moisturizer according to claim 1, wherein the skin moisturizer is moisturized by an action of promoting keratin moisture recovery that recovers keratin moisture reduced by ultraviolet irradiation. The oral skin moisturizer according to claim 1 or 2 , further comprising collagen.