JP6406655B2 - Method for softening vegetable food material, softened preparation, softened vegetable food material and food using the same - Google Patents
Method for softening vegetable food material, softened preparation, softened vegetable food material and food using the same Download PDFInfo
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Description
本発明は食品、特に植物性食品素材の軟化に関するものである。 The present invention relates to the softening of foods, particularly vegetable food materials.
近年、高齢化社会や医療の進歩等の要因による健康状態の多様化により、様々な状態の食品を必要とする人が多数存在するようになった。 In recent years, due to diversification of health conditions due to factors such as an aging society and medical progress, there are many people who need foods in various states.
食品の状態の中には、介護食といわれる食品の状態があり、該介護食は、加齢に伴いそれまでの健常食が食べ辛くなった人や、手術後や一過的な健康状態の変化によって、一時的にこのような食事を必要とする人に供される。 Among food states, there is a state of foods called care foods, which can be used for people who have difficulty eating healthy foods as they age, or after surgery or temporary health conditions. Due to change, it is temporarily provided to those who need such a meal.
これらの中には、食品を包丁やフードカッター等で細かく刻んだ食事である「刻み食」や、ミキサーなどですりつぶしたペースト状の「ミキサー食」などがある。 Among these, there are “chopped meals” that are meals that are finely chopped with a knife or food cutter, etc., and “mixer meals” that are pasted with a mixer or the like.
これらのうち、嚥下障害などを持たない高齢者や、単に咀嚼機能の低下によって介護食を必要とする人には「刻み食」が供されることがあるが、「刻み食」は単に食品が小さくなっているにすぎず、飲み込みを行なうためには少なからず咀嚼が必要である。そのうえ、食品は小さく刻まれ、もともとの形状を保持していない。 Of these, chopped meals may be provided to elderly people who do not have dysphagia, or to people who need nursing food simply due to a decrease in masticatory function. It is only small and requires a bit of chewing to swallow. In addition, the food is chopped small and does not retain its original shape.
一方、「ミキサー食」については咀嚼機能がかなり低下し、口中で食品を咀嚼することが出来ない人でも摂取することができるが、その形状はゾル状で、もともとの食品の形状を保持していないばかりか、見栄えが悪く、味も劣る。 On the other hand, “mixer food” has a considerably reduced chewing function and can be ingested even by people who cannot chew food in the mouth, but its shape is sol and retains the shape of the original food. Not only does it look bad, but also tastes poor.
これは、介護食を食べる人の食欲低下の原因ともなり、本来、介護食を食べることによって、体力を維持・回復すべき人らに、食事による充分な恩恵をあたえられない、という問題につながっていた。 This is also a cause of decreased appetite for those who eat nursing food, and leads to the problem that by eating nursing food, people who should maintain and recover their physical strength cannot be given sufficient benefits from eating. It was.
そのため、咀嚼機能が低下した人でも食べることができる軟らかさでありながら、「刻み食」、「ミキサー食」より、見栄えの良い、つまり見た目が健常食と変わらない形状のままの「軟菜食」という新たな介護食が望まれていた。「軟菜食」は通常の食事を取ることが出来ない人にも食べやすく、なおかつ食事に楽しみを持たせることができることから、心身の健康に寄与することができるが、そのためには、通常の調理では軟らかくなりにくい食品素材を、形状を保ったまま軟らかくする技術が必要であった。 Therefore, it is soft enough to be eaten even by people with reduced chewing function, but it looks better than “chopped meal” and “mixer meal”, that is, “soft vegetable meal” that has a shape that does not change from that of healthy food. A new nursing food was desired. “Soft vegetable foods” are easy to eat even for people who cannot eat a normal meal, and can make the meal enjoyable, so it can contribute to mental and physical health. Therefore, a technology to soften food materials that are difficult to soften while maintaining their shape was necessary.
これまでに、このような食品の製造方法について、さまざまな検討がなされてきた。一般に、このような軟菜食を通常の調理により作る工夫としては、圧力鍋による加熱を行なうことが通常であったが長時間の加熱が必要であった。 So far, various studies have been made on methods for producing such foods. In general, as a device for making such soft vegetable foods by ordinary cooking, heating with a pressure cooker was normal, but heating for a long time was required.
そこで、例えば、特開2003−284522号公報(特許文献1)では、植物食品素材を凍結、解凍後、酵素液中で減圧状態とすることにより短時間の酵素処理で軟化食品を製造できる方法が示されている。 Therefore, for example, in Japanese Patent Application Laid-Open No. 2003-284522 (Patent Document 1), there is a method that can produce a softened food with a short enzyme treatment by freezing and thawing a plant food material and then reducing the pressure in an enzyme solution. It is shown.
また、特開2011−160763号公報(特許文献2)に示される方法でも酵素を食品に含浸させる方法が開示されているが、対象となる食品に対して過熱水蒸気による乾燥を行い、次いで減圧状態で酵素液に含浸させることを必要としている。 Also, a method disclosed in Japanese Patent Application Laid-Open No. 2011-160763 (Patent Document 2) discloses a method of impregnating a food with an enzyme. However, the target food is dried with superheated steam, and then in a reduced pressure state. It is necessary to impregnate the enzyme solution.
すなわち、上記に示されたような方法を用いるには減圧装置などの設備を整える必要があり、さらにはそれらの機器を操作するという通常の調理工程とは大きく異なる手間をかけなくてはいけなかった。したがって、家庭や小規模の病院および介護施設で実施することは容易ではなかった。 In other words, in order to use the method as shown above, it is necessary to arrange equipment such as a decompression device, and furthermore, it is necessary to take a time greatly different from the normal cooking process of operating those devices. It was. Therefore, it has not been easy to implement at home, small hospitals and nursing homes.
さらには、特許文献2ではトレハロースやグリセリンを、食品を軟化させるための酵素液に含有させることが示されており、これらの浸透圧を利用し、酵素の浸透を行なう方法の開示がある。 Furthermore, Patent Document 2 discloses that trehalose or glycerin is contained in an enzyme solution for softening foods, and there is a disclosure of a method for performing enzyme permeation using these osmotic pressures.
また特開2010−115164号公報(特許文献3)には酵素液に二糖を含むことで形状を維持したままで軟質化され、優れた食感を有する軟質化植物食材を提供する方法が示されている。 Japanese Patent Application Laid-Open No. 2010-115164 (Patent Document 3) discloses a method for providing a softened plant food material that is softened while maintaining its shape by containing a disaccharide in an enzyme solution and has an excellent texture. Has been.
しかし、いずれの方法も酵素液の導入を減圧工程で行なうことを示しており、より簡便に食品を軟化させる方法が求められていた。 However, both methods indicate that the introduction of the enzyme solution is performed in a decompression step, and a method for softening food more easily has been demanded.
したがって、本発明では、通常の調理方法では軟化し難い植物性食品素材でも、特殊な設備などを必要とせず、簡単な操作で、野菜の形状を保ったまま摂取しやすい食感まで軟らかくする方法を提供することを目的とする。 Therefore, in the present invention, even a vegetable food material that is difficult to soften by a normal cooking method does not require special equipment, etc., and it is a simple method to soften the food texture that is easy to ingest while maintaining the shape of vegetables. The purpose is to provide.
上記目的を達成するために鋭意検討した結果、本発明者らは、糖アルコールを用いることで、酵素の浸透性をあげ、酵素液含浸において減圧工程を経なくてもよい、簡便な植物性食品素材の軟化方法を見出し、本発明を完成するに至った。 As a result of intensive studies to achieve the above object, the present inventors have improved the permeability of the enzyme by using sugar alcohol, and a simple vegetable food that does not require a pressure reduction step in the enzyme solution impregnation. A method for softening the material has been found and the present invention has been completed.
すなわち、本発明は、第一に、糖アルコールと酵素とを植物性食品素材に含浸させることを特徴とする、植物性食品素材の軟化方法である。
第二に、植物性食品素材に糖アルコールと酵素とを用いて含浸させる前、または含浸させるときに、糖アルコールと酵素の含浸を促進させるための処理を行うことを特徴とする、上記第一に記載の植物性食品素材の軟化方法である。
第三に、糖アルコールと酵素の混合液に植物性食品素材を浸漬させることを特徴とする、上記第一または第二に記載の植物性食品素材の軟化方法である。
第四に、糖アルコールと酵素の混合液が、混合液100重量部中に15重量部乃至55重量部の糖アルコールを含有することを特徴とする、上記第三に記載の植物性食品素材の軟化方法である。
第五に、糖アルコールが、ソルビトール、還元澱粉糖化物、マルチトールからなる群から選ばれる1種または2種以上であることを特徴とする、上記第一から第四のいずれか一つに記載の植物性食品素材の軟化方法である。
第六に、糖アルコールと酵素とを有効成分とする、植物性食品素材の軟化製剤である。
第七に、上記第一から第五のいずれか一つに記載の方法により得られた植物性食品素材である。
第八に、上記第七に記載の植物性食品素材を用いた食品である。
That is, the present invention is a method for softening a vegetable food material, characterized by first impregnating a vegetable food material with a sugar alcohol and an enzyme.
Second, the plant food material is subjected to a treatment for promoting impregnation of the sugar alcohol and the enzyme before or when impregnated with the sugar alcohol and the enzyme. The method for softening a vegetable food material described in 1.
Third, the vegetable food material softening method according to the first or second aspect, wherein the vegetable food material is immersed in a mixed solution of sugar alcohol and enzyme.
Fourthly, the mixed solution of sugar alcohol and enzyme contains 15 parts by weight to 55 parts by weight of sugar alcohol in 100 parts by weight of the mixed liquid. Softening method.
Fifth, the sugar alcohol is one or two or more selected from the group consisting of sorbitol, reduced starch saccharified product, and maltitol. This is a method for softening vegetable food materials.
Sixth, it is a softened preparation of plant food material containing sugar alcohol and enzyme as active ingredients.
Seventh, a vegetable food material obtained by the method according to any one of the first to fifth aspects.
Eighth, it is a food using the vegetable food material described in the seventh.
以下に本発明の構成について、詳細に説明する。 The configuration of the present invention will be described in detail below.
本発明でいう植物性食品素材とは、植物性の食物で、人が日常的に摂取するものであり、じかに摂取できるものと加工調理しなければ摂取できないものがある。さらに、本発明でいう食品とは、その状態のまま摂取可能であるものであり、生で摂取可能な植物性食品素材や加工調理によって摂取できるようにした植物性食品素材を含むものである。 The plant food material referred to in the present invention is a vegetable food that is ingested daily by humans, and can be ingested directly or can be ingested only by processing. Furthermore, the foods referred to in the present invention are those that can be ingested as they are, and include plant food materials that can be ingested raw and plant food materials that can be ingested by processing and cooking.
本発明によれば、植物性食品素材を「摂取しやすい食感」まで軟らかくすることが出来る。「摂取しやすい食感」と表現される硬さとは、食品を咀嚼する力が弱く、硬い食品や大きい食品が食べづらい人が、簡単に咀嚼できる程度、または舌や歯茎などで押しつぶすことができる程度のかたさをいう。 According to the present invention, the vegetable food material can be softened to “easy to eat”. Hardness expressed as “easy to eat” means that the ability to chew foods is weak and can be easily chewed by people who are hard to eat hard or large foods, or can be crushed with the tongue or gums Degree of hardness.
また、本発明において、植物性食品素材とは、特に限定されるものではないが、野菜(根菜、茎菜、果菜、葉物、茎葉菜、花芽・芽もの野菜、イモ)類、果実類、豆類、穀類、種実(ナッツ)類、藻類、きのこ類などが例示され、これらの1種以上の組み合わせからなってもよい。 In the present invention, the vegetable food material is not particularly limited, but includes vegetables (root vegetables, stem vegetables, fruit vegetables, leaves, stem leaves, flower buds, bud vegetables, potatoes), fruits, Beans, cereals, seeds (nuts), algae, mushrooms and the like are exemplified, and may be a combination of one or more of these.
ここで、野菜類としては、一般に根菜といわれる大根、ニンジン、キントキニンジン、ゴボウ、カブなど、茎菜といわれるレンコン、タケノコ、アスパラガス、ウドなど、果菜といわれるキュウリ、ナス、トマト、ズッキーニ、カボチャ、ピーマン、パプリカ、シシトウ、トウガラシ、オクラ、トウガン、ヒョウタン、ヒルガオ、マクワウリ、シロウリ、ニガウリ、ヘチマ、オリーブ、トウモロコシなど、葉物といわれる、ホウレンソウ、小松菜、カラシナ、アブラナ、キャベツ、レタス、白菜、青梗菜、春菊、フキ、リーキ、高菜、野沢菜、ツケナ、ウキナ、ミブナ、水菜、キョウナ、カイラン、アシタバ、モロヘイヤ、ツルムラサキ、空芯菜、菜心、コウサイタイ、セリ、ナズナ、ゴギョウ、ハコベラ、ホトケノザ、タアサイ、千宝菜、リーフレタス、プリーツレタス、ロメインレタス、茎レタス、サラダ菜、パセリ、ミツバ、シソ、セロリ、ルッコラ、クレソンなど、茎葉菜といわれるネギ、アサツキ、ワケギ、ニラ、オカヒジキ、タマネギ、ラッキョウ、エシャロット、ユリネ、クワイ、キクイモ、コールラビ、ザーサイ、ニンニク、ショウガなど、花菜・芽もの野菜といわれるブロッコリー、カリフラワー、ナノハナ、アーチチョーク、キク、アザミ、フキノトウ、ノビル、ゼンマイ、蕨、タラノメ、土筆、チコリー、モヤシ、各種スプラウトなど、および、イモ類といわれるジャガイモ、サトイモ、サツマイモ、ヤマトイモ、イチョウイモ、長イモ、ヤマイモ、自然薯、ムカゴ、タロイモ、ヤツガシラなどが挙げられる。 Here, as vegetables, radish, carrot, quinto carrot, burdock, turnip, etc., which are commonly referred to as root vegetables, lotus roots, bamboo shoots, asparagus, udo, etc., such as stem vegetables, cucumbers, eggplants, tomatoes, zucchini, pumpkins, etc. , Peppers, paprika, shrimp, capsicum, okra, toucan, gourd, convolvulus, makuwauri, shirori, bitter gourd, loofah, olives, corn, etc. Chinese cabbage, spring chrysanthemum, fuki, leek, takana, nozawana, tsukena, ukina, mibuna, mizuna, kyouna, kairan, ashitaba, morohaya, tsurumura, air-core, vegetarian, kosaitai, seri, nazuna, gogyo, cocobella, hotokenoza, Taasai, Chihona, -Fretas, pleated lettuce, romaine lettuce, stem lettuce, salad vegetables, parsley, honey bees, perilla, celery, arugula, watercress, etc. Broccoli, cauliflower, nanohana, arch choke, chrysanthemum, thistle, fukinotou, nobil, springfish, persimmon, taranome, earthenware brush, chicory, sprout, various sprout, etc. Moreover, potatoes, taros, sweet potatoes, yam potatoes, ginkgo potatoes, long potatoes, yams, natural pods, mud potatoes, taros, wild squirrels etc., which are referred to as potatoes, can be mentioned.
また、果実類としては、リンゴ、柿、梨、洋ナシ、花梨、バナナ、パイナップル、ライチ、マンゴ、パパイヤ、マゴスチン、ランブータン、ロンガン、イチゴ、ブルーベリー、ブドウ、マスカット、枇杷、杏、白桃、黄桃、プラム、メロン、スイカ、オレンジ、みかん、イヨカン、ボンタン、タンカン、キンカン、グレープフルーツ、アケビ、アセロラ、アボカド、無花果、ウメ、キウイフルーツ、グアバ、サクランボ、スターフルーツ、チェリモヤ、ドラゴンフルーツ、ドリアン、パッションフルーツなど、豆類としては、ダイズ、アズキ、ソラマメ、ヒヨコマメ、ハナマメ、エンドウマメ、インゲンマメ、白インゲン、莢インゲン、莢エンドウ、スナックエンドウなどが挙げられる。さらに穀類としては、白米、黒米、もち米、大麦、ハダカムギ、ハトムギ、オーツムギ、ソバノミ、ヒエ、アワ、キビ、キヌア、アマランサスなど、種実類としては、落花生、アーモンド、カシューナッツ、マカダミアナッツ、ピスタチオ、胡桃、栗、銀杏、クコ、ケシノミ、マツノミなど、藻類としては、わかめ、昆布、メカブ、ヒジキ、紅藻など、きのこ類としては、しめじ、しいたけ、エノキダケ、まいたけ、エリンギ、ナメコ、キクラゲ、マッシュルーム、フクロタケ、ヒラタケ、ヤマブシタケ、キヌガサタケ、マツタケ、トリュフなどが挙げられる。 In addition, fruits include apples, strawberries, pears, pears, pears, bananas, pineapples, litchis, mangoes, papayas, magosteens, rambutans, longan, strawberries, blueberries, grapes, muscat, strawberries, apricots, white peaches, yellow Peach, Plum, Melon, Watermelon, Orange, Tangerine, Yoyokan, Bontang, Tankan, Kumquat, Grapefruit, Akebi, Acerola, Avocado, No Fruit, Ume, Kiwi Fruit, Guava, Cherry, Star Fruit, Cherimoya, Dragon Fruit, Durian, Passion Examples of beans such as fruit include soybeans, azuki bean, broad beans, chickpeas, peas, peas, kidney beans, white beans, grape beans, peas, and snack peas. In addition, cereals include white rice, black rice, glutinous rice, barley, hadakamugi, pearl barley, oats, buckwheat, millet, millet, quinoa, amaranth, and the like seeds include peanuts, almonds, cashew nuts, macadamia nuts, pistachios, and walnuts. , Chestnuts, ginkgo, wolfberry, poppy flea, pine chisel, algae, seaweed, kelp, mekabu, hijiki, red algae, mushrooms, shimeji mushroom, shiitake mushroom, mushroom, maitake mushroom, eringi, sea cucumber, mushroom, mushroom, bukurotake , Oyster mushrooms, yamabushitake, kinugasatake, matsutake and truffles.
この中でも、好適には、野菜類、果実類、豆類、藻類およびきのこ類が、特に好適には、根菜・茎菜・果菜・葉物・花菜・イモなどの野菜類が、本発明における植物性食品素材として使用できる。 Among these, preferably, vegetables, fruits, beans, algae and mushrooms, particularly preferably vegetables such as root vegetables, stem vegetables, fruit vegetables, leaves, flower vegetables, potatoes, etc. Can be used as a food material.
さらに、本発明の方法に用いる植物性食品素材は、生のままでもよいが、冷凍されたものや調理されたものでもよい。 Furthermore, the vegetable food material used in the method of the present invention may be raw, but may be frozen or cooked.
本発明において、使用できる糖アルコールとしては、マルチトール、キシリトール、エリスリトール、ソルビトール、マンニトール、ラクチトール、還元パラチノース、還元澱粉糖化物などを挙げることができ、粉状、顆粒状、シロップ状などその状態を問わず使用できる。さらに、糖アルコールは2種以上を組み合わせてもよい。 Examples of the sugar alcohol that can be used in the present invention include maltitol, xylitol, erythritol, sorbitol, mannitol, lactitol, reduced palatinose, reduced starch saccharified product, and the like such as powder, granule, and syrup. Can be used regardless. Furthermore, two or more sugar alcohols may be combined.
また、この中でもソルビトール、還元澱粉糖化物、マルチトールからなる群から選ばれる1種または2種以上の糖アルコールが本発明において好適に使用でき、さらにはソルビトールおよび/またはマルチトールを全糖アルコール中の10%以上含むものがさらに好適に使用でき、60%以上含むものが特に好適である。 Of these, one or more sugar alcohols selected from the group consisting of sorbitol, reduced starch saccharified product, and maltitol can be preferably used in the present invention. Furthermore, sorbitol and / or maltitol is used in the total sugar alcohol. Those containing 10% or more can be used more preferably, and those containing 60% or more are particularly preferred.
本発明において、糖アルコールと酵素とを植物性食品素材に含浸させる方法は、糖アルコールと酵素とを植物性食品素材と混和・混捏させる、植物性食品素材を浸漬させる、植物性食品素材に散布・塗布・被覆・噴霧・注入させるなどの従来公知のいずれの方法でも良いものであり、糖アルコールおよび酵素は、液状であっても、粉状であっても良い。ただし、含浸処理の容易性や浸透性の観点から、糖アルコールおよび酵素が液状であることが好ましい。さらに、糖アルコールおよび酵素を前もって水などの溶媒に分散させて混合液として植物性食品素材に含浸させることが浸透性の観点からより好ましい。しかしながら、結果として糖アルコールと酵素とを植物性食品素材において混合できれば、糖アルコールと酵素とを別個に植物性食品素材に含浸させることもできる。 In the present invention, the method for impregnating a vegetable food material with a sugar alcohol and an enzyme includes mixing the sugar alcohol and the enzyme with the vegetable food material, immersing the vegetable food material, spraying the vegetable food material. Any conventionally known method such as coating, coating, spraying, and pouring may be used, and the sugar alcohol and enzyme may be liquid or powdery. However, it is preferable that the sugar alcohol and the enzyme are liquid from the viewpoint of easy impregnation treatment and permeability. Furthermore, it is more preferable from the viewpoint of permeability to disperse the sugar alcohol and the enzyme in a solvent such as water in advance and impregnate the vegetable food material as a mixed solution. However, if the sugar alcohol and the enzyme can be mixed in the vegetable food material as a result, the sugar alcohol and the enzyme can be impregnated separately in the vegetable food material.
また、本発明において糖アルコールと酵素を混合液として使用する場合には、糖アルコールは混合液の全体重量100重量部に対して、糖アルコールの固形分として15重量部から55重量部の範囲で含有することができ、特に好適には20重量部から45重量部の範囲で含有することができる。ただし、15重量部未満の場合は、酵素の浸透に対して、糖アルコールの効果が低く、また55重量部より多い場合は糖アルコールの甘味が強く出て、植物性食品素材に対する調味にふさわしくない影響がある。 In the present invention, when sugar alcohol and an enzyme are used as a mixed solution, the sugar alcohol is in a range of 15 to 55 parts by weight as a solid content of the sugar alcohol with respect to 100 parts by weight of the whole mixed solution. It can be contained, particularly preferably in the range of 20 to 45 parts by weight. However, when the amount is less than 15 parts by weight, the effect of the sugar alcohol is low on the permeation of the enzyme, and when the amount is more than 55 parts by weight, the sweetness of the sugar alcohol is so strong that it is not suitable for seasoning to the vegetable food material. There is an impact.
一方、本発明において使用できる酵素はセルラーゼ、ヘミセルラーゼ、ペクチナーゼ、キチナーゼ、プロテアーゼ、アミラーゼ、リパーゼなどを挙げることができ、粉状、顆粒状、溶液状、懸濁液状などその状態を問わず使用できる。さらに酵素は2種以上を組み合わせても良く、好適には、セルラーゼ、ヘミセルラーゼ、ペクチナーゼを使用することが出来る。 On the other hand, enzymes that can be used in the present invention include cellulase, hemicellulase, pectinase, chitinase, protease, amylase, lipase, etc., and can be used in any state such as powder, granule, solution, suspension, etc. . Furthermore, two or more enzymes may be combined, and cellulase, hemicellulase, and pectinase can be preferably used.
また、本発明における酵素の量は、軟化させる植物性食品素材の種類、生であるか、冷凍であるか、調理済みであるか等の植物性食品素材の状態、さらに、含浸を促進させる処理の有無などによっても異なるため、特に限定されない。ただし、酵素が少なすぎると、糖アルコールの酵素浸透作用をもってしても、植物性食品素材の軟化が困難であることが多く、酵素が多くなりすぎると高濃度の酵素のため、軟化させる植物性食品素材の種類によっては、形状が崩れるといった見た目上ふさわしくない問題が起こる可能性が高いため、軟化させる植物性食品素材の重量に対し酵素を0.03重量%から0.5重量%の範囲で使用することが好ましく、0.05重量%から0.5重量%の範囲で使用することがさらに好ましい。 In addition, the amount of the enzyme in the present invention is the kind of the vegetable food material to be softened, the state of the vegetable food material such as raw, frozen, or cooked, and the treatment for promoting the impregnation. Since it varies depending on the presence or absence, etc., there is no particular limitation. However, if there are too few enzymes, it is often difficult to soften vegetable food materials even if the sugar alcohol has an enzyme penetration action. Depending on the type of food material, there is a high possibility that an undesired problem that the shape will collapse will occur, so the enzyme content is in the range of 0.03% to 0.5% by weight based on the weight of the vegetable food material to be softened. It is preferable to use it, and it is more preferable to use it in the range of 0.05% by weight to 0.5% by weight.
ここでいう植物性食品素材の重量とは、表面に付着した水分や調味液などの液体をザルやペーパータオルなどを用いて、したたらない状態まで除いたものであるが組織内の水分は保持したままの状態で、測定した場合の重量である。 The weight of the vegetable food material here refers to the water or seasoning liquid adhering to the surface removed using a colander or paper towel, etc., until it is not dripping, but the moisture in the tissue was retained. It is the weight when measured as it is.
さらに、糖アルコールと酵素を含浸させる植物性食品素材は、生のままでも冷凍されたものでもよく、また下ごしらえとして単純にゆでる・蒸すなどの加熱処理を施してあるものでも、調理してあるものでもよいが、糖アルコールと酵素を含浸させる際には酵素が作用する範囲の温度としておくことが好ましく、植物性食品素材の温度が0℃〜50℃、さらには10℃〜40℃であることが好ましい。 In addition, plant food materials impregnated with sugar alcohol and enzymes can be raw or frozen, or simply cooked or steamed as a preparation, or cooked. However, when impregnating the sugar alcohol and the enzyme, it is preferable to set the temperature within a range where the enzyme acts, and the temperature of the vegetable food material is 0 ° C. to 50 ° C., further 10 ° C. to 40 ° C. Is preferred.
また、植物性食品素材は糖アルコールと酵素とを含浸させる前、または、含浸させるときに含浸を促進させるための処理を行うことができる。ここで言う含浸を促進させるための処理とは、針状のもので植物性食品素材の表面を刺すテンダライズといわれる処理、酵素が表面によく付着するように、植物性食品素材に糖アルコールと酵素とをもみこむ、塗る、容器内で糖アルコールと酵素とを植物性食品素材をあわせて振るといったタンブリングといわれる処理、糖アルコールと酵素とを植物性食品素材の内部に拡散させるように、注射器などを用いて植物性食品素材の内部へ注入するインジェクションといわれる処理、隠し包丁や包丁目などを入れるといった古くから調味料のしみこみを良くするために行なわれていた調理技術上の処理といった各種の処理が挙げられる。特に好適には、テンダライズ、タンブリングといった植物性食品素材の表面から含浸を促進させるための処理が好適に用いられる。 Further, the vegetable food material can be subjected to a treatment for promoting the impregnation before or when impregnating the sugar alcohol and the enzyme. The treatment for accelerating impregnation here is a process called tenderization that pierces the surface of the vegetable food material with a needle-like material, sugar alcohol and enzyme on the vegetable food material so that the enzyme adheres well to the surface. A so-called tumbling process in which the sugar alcohol and enzyme are shaken together with the vegetable food material in the container, a syringe, etc., to diffuse the sugar alcohol and enzyme into the vegetable food material. Various treatments such as the treatment called injection that is injected into the inside of vegetable food materials using savory, cooking technology treatment that has been performed to improve the penetration of seasonings such as putting in hidden knives and kitchen knives Is mentioned. Particularly preferably, a treatment for promoting impregnation from the surface of the vegetable food material such as tenderization or tumbling is preferably used.
本発明において糖アルコールと酵素を混合液として使用し、植物性食品素材を糖アルコールと酵素の混合液に浸漬させる場合には、糖アルコールと酵素の混合液の量は特には限定されず、植物性食品素材の表面が糖アルコールと酵素の混合液に浸かる面積が多いほどよい。ただし、植物性食品素材の種類によっては植物性食品素材から出る水分によって糖アルコールと酵素の混合液が薄まることもあるため、糖アルコールと酵素の混合液と植物性食品素材を重量の比で、糖アルコールと酵素の混合液/植物性食品素材が大きいほど好ましく、0.3以上、より好ましくは1以上となることが好ましい。 In the present invention, when the sugar alcohol and the enzyme are used as a mixed solution and the vegetable food material is immersed in the mixed solution of the sugar alcohol and the enzyme, the amount of the mixed solution of the sugar alcohol and the enzyme is not particularly limited. The more the surface of the functional food material is immersed in the mixture of sugar alcohol and enzyme, the better. However, depending on the type of vegetable food material, the mixture of sugar alcohol and enzyme may be diluted by moisture from the vegetable food material. The larger the mixture of sugar alcohol and enzyme / vegetable food material is, the more preferable it is, and it is preferably 0.3 or more, more preferably 1 or more.
また、植物性食品素材に糖アルコールと酵素を含浸させる間は、一般に酵素の処理を施す際に通常用いられる温度帯で保管すればよいが、30℃〜50℃の範囲であれば、酵素の活性が高く維持されるため、短時間で植物性食品素材を摂取しやすく軟化させることができるため好ましい。ただし、冷蔵庫内のような3℃〜10℃の低温であっても軟化植物性食品素材は得られる。一方、3℃未満の低温では酵素の凍結や酵素の活性が低く抑えられることから好ましくないし、50℃より高い温度帯では酵素の失活や、野菜の硬化がおこり易く、好ましくない。 Moreover, while impregnating the vegetable food material with the sugar alcohol and the enzyme, it is generally stored in a temperature range usually used when the enzyme treatment is performed. Since the activity is maintained high, it is preferable because the vegetable food material can be easily ingested and softened in a short time. However, the softened vegetable food material can be obtained even at a low temperature of 3 ° C to 10 ° C as in the refrigerator. On the other hand, a low temperature of less than 3 ° C. is not preferable because enzyme freezing and enzyme activity are kept low, and a temperature range higher than 50 ° C. is not preferable because enzyme inactivation and vegetable hardening tend to occur.
植物性食品素材に糖アルコールと酵素を含浸させる時間は、植物性食品素材の種類や切り方、生であるか、冷凍や加熱処理といった下ごしらえがしてあるか、含浸を促進させる処理の有無という状態の違い、酵素の量、糖アルコールの量、糖アルコールと酵素を含浸させる間の保管温度でも異なるが、30分以上で植物性食品素材を摂取しやすく軟化させることができ、3時間以上あればより好ましい。ただし、長時間にわたって植物性食品素材に糖アルコールと酵素を含浸させていると、野菜の種類によっては、その形状が崩れてくるものもあるし、24時間を越えると、雑菌の繁殖等の衛生上の問題から好ましくない。 The time to impregnate the vegetable food material with sugar alcohol and enzyme is the type and cutting method of the vegetable food material, whether it is raw, prepared by freezing or heat treatment, or whether there is a treatment that promotes impregnation The difference in state, the amount of enzyme, the amount of sugar alcohol, and the storage temperature between impregnation of sugar alcohol and enzyme are also different, but in 30 minutes or more, vegetable food materials can be easily ingested and softened, and there are more than 3 hours More preferable. However, if the vegetable food material is impregnated with sugar alcohol and enzyme for a long time, depending on the type of vegetable, the shape may collapse, and if it exceeds 24 hours, hygiene such as propagation of various bacteria It is not preferable because of the above problem.
また、植物性食品素材に糖アルコールとともに含浸させた酵素は、含浸時間の終了時には、加熱により酵素失活させるが、加熱の方法は煮る・蒸す・焼く・揚げるなど通常の調理のいずれの方法でもよいし、スチームコンベクションオーブンなどの機器を用いて加熱を行なってもよい。加熱の温度は特に限定されるものではなく、一般に酵素失活に用いられる温度帯で行なえばよいため、70℃以上でよく、95℃以上の高温であれば、殺菌の効果も得られるため好ましいが、植物性食品素材から水分が抜けすぎる、焦げ付くなどして硬くならないようにすることが望ましく、170℃以下の温度範囲で行なうことが望ましい。 In addition, the enzyme impregnated with the sugar alcohol in the vegetable food material is deactivated by heating at the end of the impregnation time, but the heating method can be any method of normal cooking such as boiling, steaming, baking, frying Alternatively, heating may be performed using a device such as a steam convection oven. The heating temperature is not particularly limited, and may be performed in a temperature range generally used for enzyme deactivation, and may be 70 ° C. or higher, and a high temperature of 95 ° C. or higher is preferable because a sterilizing effect can be obtained. However, it is desirable to prevent moisture from being removed from the vegetable food material so that it does not become hard due to scorching, etc., and it is desirable to carry out in a temperature range of 170 ° C. or lower.
また、本発明において糖アルコールと酵素とを有効成分とする植物性食品素材の軟化製剤に含まれる成分は、有効成分としての糖アルコールと酵素の他に、これらを分散させるための水、アルコール類などの液体や、賦形剤としてデキストリンや、増粘多糖類を含んでもよい。 In addition, in the present invention, the components contained in the softened preparation of the vegetable food material containing sugar alcohol and enzyme as active ingredients include, in addition to sugar alcohol and enzyme as active ingredients, water and alcohols for dispersing them. Or dextrin or a thickening polysaccharide as an excipient.
さらに、これら以外に必要であれば本発明の目的が達成される限りにおいて他の原料も配合することができ、しょうゆ、みりん、砂糖、味噌、塩、酒類、香辛料といった一般的な調味料や、異性化糖、高甘味剤などの甘味料、グルタミン酸塩、イノシン酸塩といったアミノ酸、さらには、酵母エキス、魚介エキス、畜肉エキス、野菜エキス、海藻エキスなどの各種エキス、旨み付与のためのたんぱく加水分解物、核酸、有機酸、無機塩、発酵調味料や酸味料、各種食材のペーストまたは粉末・微細物などの呈味材料のほか、pH調整剤、油脂類、増粘安定剤、乳化剤、酸化防止剤、日持ち向上剤、着色料、ビタミンなどの栄養強化剤、香料などいわゆる調味液に使われる各種原料を含むこともできる。また、これらの調味料、調味液原料は粉状、顆粒状、微粒状、液状、ペースト状等のいずれの状態でもよい。 In addition to these, as long as the object of the present invention is achieved, other ingredients can be blended, soy sauce, mirin, sugar, miso, salt, alcoholic beverages, spices, and other common seasonings, Isomerized sugar, sweeteners such as high sweeteners, amino acids such as glutamate and inosinate, and various extracts such as yeast extract, seafood extract, livestock meat extract, vegetable extract and seaweed extract, Degradation products, nucleic acids, organic acids, inorganic salts, fermented seasonings and sour seasonings, flavoring materials such as pastes of various ingredients, powders and fine products, pH adjusters, fats and oils, thickening stabilizers, emulsifiers, oxidation Various raw materials used for so-called seasoning liquids, such as inhibitors, shelf life improvers, coloring agents, nutritional fortifiers such as vitamins, and fragrances can also be included. These seasonings and seasoning liquid materials may be in any state such as powder, granule, fine granule, liquid, and paste.
本発明によると、減圧装置などの特殊な設備などを必要とせず、簡単な操作で、野菜の形状を保ったまま摂取しやすい食感まで軟らかくする方法を提供する事ができる。 According to the present invention, it is possible to provide a method of softening a texture that is easy to ingest while maintaining the shape of vegetables with a simple operation without requiring special equipment such as a decompression device.
以下に、実施例を示して本発明を詳細に説明するが、本発明は以下の実施例に限定されるものではない。 EXAMPLES Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the following examples.
実施例において使用した酵素はアミラーゼを使用した場合は、天野エンザイム株式会社製の製品名「クライスターゼE5」および「グルクザイムAF6」を併せて使用し、含まれる酵素量は、賦形剤の配合比率から算出した。酵素製剤を用いた場合はすべて三菱商事フードテック株式会社製「ソフトベジタブルA」であり、成分として含まれる酵素はペクチナーゼとヘミセルラーゼからなり、賦形剤が含まれる。酵素量は酵素製剤全重量の3.9重量%である。 When amylase is used as the enzyme used in the examples, the product names “Chrytase E5” and “Gluczyme AF6” manufactured by Amano Enzyme Co., Ltd. are used together, and the amount of the enzyme contained is the mixing ratio of the excipient Calculated from When the enzyme preparation is used, all are "Soft Vegetable A" manufactured by Mitsubishi Corporation Foodtech Co., Ltd. The enzyme contained as a component is composed of pectinase and hemicellulase, and includes excipients. The amount of enzyme is 3.9% by weight of the total weight of the enzyme preparation.
<本願におけるかたさ測定方法>
かたさの測定は、株式会社山電製のクリープメーター(モデル:RE2−3305B)を使用した。測定条件は、実施例13以外はテクスチャー測定モード、実施例13が破断強度測定モードを使用した。また、実施例3〜8、12、14〜19は20mm直径のプランジャー、実施例9、11、13は3mm直径のプランジャー、実施例10は5mm直径のプランジャーを使用した。試料圧縮速度は、テクスチャー測定モードでは10mm/秒、破断強度測定モードでは1mm/秒とした。測定時、キュウリ、大根、ゴボウ、インゲンは自立しないため高さ15mm、直径40mmの測定セルに入れたが、ジャガイモ、白菜の芯の部分、レンコン、ブロッコリー、エリンギ、リンゴ、コンブは測定台に直接測定方向を上にして置くことができるため、測定セルには入れなかった。また白菜の葉の部分は測定セルの高さまで重ねて入れた。プランジャーはテクスチャー測定モードでは試料を測定セルに入れた場合10mm押し込むように測定したが、ジャガイモ、白菜の芯、レンコン、ブロッコリー、エリンギ、リンゴは試料の高さの66.67%の高さまで圧縮するよう測定し、応力ピークの最高点をかたさの測定値とした。また、破断強度測定モードでは、試料の高さの99%の高さまで圧縮するよう測定し、破断点の応力をかたさの測定値とした。なお、測定時の試料は全て20℃となるよう、酵素を失活させたのちに冷却した。
<Method for measuring hardness in the present application>
The hardness was measured using a Yamaden Corporation creep meter (model: RE2-3305B). The measurement conditions were the texture measurement mode except for Example 13, and Example 13 used the break strength measurement mode. Examples 3-8, 12, and 14-19 used 20 mm diameter plungers, Examples 9, 11, and 13 used 3 mm diameter plungers, and Example 10 used 5 mm diameter plungers. The sample compression speed was 10 mm / second in the texture measurement mode and 1 mm / second in the breaking strength measurement mode. During measurement, cucumbers, radishes, burdock, and green beans were placed in a measuring cell with a height of 15 mm and a diameter of 40 mm because they were not self-supporting. Since it could be placed with the measurement direction facing up, it was not placed in the measurement cell. Also, the Chinese cabbage leaves were stacked up to the height of the measurement cell. In the texture measurement mode, the plunger was measured to push 10 mm when the sample was placed in the measurement cell, but the potato, Chinese cabbage core, lotus root, broccoli, eringi, and apple were compressed to 66.67% of the sample height. The highest point of the stress peak was taken as the hardness measurement value. In the break strength measurement mode, the sample was measured to be compressed to a height of 99% of the height of the sample, and the stress at the break point was measured as the hardness. In addition, it cooled, after inactivating the enzyme so that all the samples at the time of measurement might be 20 degreeC.
酵素と、糖または糖アルコールを混合しない条件(比較例3〜6、7−1、8〜12、14−1、17〜19)で酵素液を含浸させた軟化植物性食品素材のかたさを、含浸方法、酵素量、植物性食品素材種類ごとにそれぞれ100として、糖アルコールと酵素の混合液を含浸させたそれぞれの軟化植物性食品素材のかたさをXであらわし、各条件の軟化植物性食品素材がどの程度軟らかくなっているかを軟化度として下記の式1で表した。 The hardness of the softened vegetable food material impregnated with the enzyme solution under conditions where the enzyme and sugar or sugar alcohol are not mixed (Comparative Examples 3-6, 7-1, 8-12, 14-1, 17-19) Each impregnation method, amount of enzyme, and vegetable food material type is set to 100, and the softness vegetable food material impregnated with a mixture of sugar alcohol and enzyme is represented by X, and the softened vegetable food material of each condition The degree of softening is expressed by the following formula 1 as the degree of softening.
(式1) 軟化度=100−X (Formula 1) Softening degree = 100-X
<糖アルコールによる酵素の浸透促進効果の確認> <Confirmation of the effect of sugar alcohol to promote enzyme penetration>
[実施例1]
植物性食品素材としてジャガイモを使用した。ジャガイモを20mm角に切り、5分間下ゆでしたものを用意した。酵素は、アミラーゼを使用し、酵素量は0.0356重量部となるようにした。さらに、マルチトールを20重量部、水を79.938重量部混合して糖アルコールと酵素の混合液を作成した。マルチトールは三菱商事フードテック株式会社製の製品名「レシス」を使用した。以降もマルチトールを使用する場合は同様である。
[Example 1]
Potato was used as a vegetable food material. Potatoes were cut into 20 mm squares and prepared for 5 minutes. As the enzyme, amylase was used, and the amount of the enzyme was 0.0356 parts by weight. Furthermore, 20 parts by weight of maltitol and 79.938 parts by weight of water were mixed to prepare a mixture of sugar alcohol and enzyme. Maltall used the product name “Resis” manufactured by Mitsubishi Corporation Foodtech. Thereafter, the same applies when using maltitol.
作成した糖アルコールと酵素の混合液に、液と同重量の上記のジャガイモを浸漬させ40℃で3時間保管し、糖アルコールと酵素の混合液を含浸させたのち、スチームコンベクションオーブンを使用して100℃で10分加熱して酵素を失活させた。 In the prepared mixture of sugar alcohol and enzyme, the above potato of the same weight as the liquid is immersed and stored at 40 ° C. for 3 hours, impregnated with the mixture of sugar alcohol and enzyme, and then using a steam convection oven. The enzyme was inactivated by heating at 100 ° C. for 10 minutes.
以上の方法で軟化ジャガイモを作成したところ、軟化の前後でジャガイモの形状は保たれたままであった。そして、該軟化ジャガイモの表面を薄く切除した残余を軟化ジャガイモ中心部として試料とし、このジャガイモ中心部のグルコース量を測定した。 When the softened potato was produced by the above method, the shape of the potato was kept before and after the softening. And the residue which cut | disconnected the surface of this softened potato thinly was made into the sample as a softened potato center part, and the glucose level of this potato center part was measured.
[比較例1]
植物性食品素材としてジャガイモを使用し、糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を99.938重量部混合して酵素液を作成したこと以外は実施例1と同じ方法でジャガイモに酵素液を含浸させたのち、同じ方法で酵素失活させ、ジャガイモ中心部のグルコース量を測定した。
[Comparative Example 1]
The same as Example 1 except that potato was used as a vegetable food material, and 99.938 parts by weight of water was mixed into the sugar alcohol / enzyme mixed solution instead of containing maltitol. After impregnating potato with an enzyme solution by the method, the enzyme was inactivated by the same method, and the amount of glucose at the center of the potato was measured.
グルコース量の測定は、試料をミキサーで破砕して搾汁を得、この搾汁から濾過、遠心分離によって懸濁物質を除去した上清をキャピラリー電気泳動システムによって測定した。 For the measurement of the amount of glucose, the sample was crushed with a mixer to obtain squeezed juice, and the supernatant from which the suspended substances were removed by filtration and centrifugation was measured with a capillary electrophoresis system.
キャピラリー電気泳動は大塚電子株式会社製、CAPI−3000を使用し、電気泳動液はピコリン酸(和光純薬工業株式会社,特級)、リン酸三ナトリウム(和光純薬工業株式会社,特級)およびHDTMA(臭化ヘキサデシルトリメチルアンモニウム、和光純薬工業株式会社,特級)をそれぞれ、5mM、10mMおよび0.25mMになるように純水に溶解し、水酸化リチウム溶液でpH12.5に調整したものを用いた。キャピラリー管は内径0.075mmで全長650mmのサイズのものを使用した。試料は落差法により落差25mmで60秒間注入し、−7kVを印加して分離した。泳動中のキャピラリーは30℃に保持した。検出および解析は、大塚電子株式会社製、CAPI−3300/3200システムを使用し、検出波長を260nmとするインダイレクトUV検出法により行ない、ピーク面積からグルコース量をもとめた。 Capillary electrophoresis uses CAPI-3000 manufactured by Otsuka Electronics Co., Ltd., and the electrophoresis solution is picolinic acid (Wako Pure Chemical Industries, Ltd., special grade), trisodium phosphate (Wako Pure Chemical Industries, Ltd., special grade), and HDTMA. (Hexadecyltrimethylammonium bromide, Wako Pure Chemical Industries, Ltd., special grade) dissolved in pure water to 5 mM, 10 mM and 0.25 mM, respectively, and adjusted to pH 12.5 with a lithium hydroxide solution Using. A capillary tube having an inner diameter of 0.075 mm and a total length of 650 mm was used. The sample was injected by a drop method at a drop of 25 mm for 60 seconds, and -7 kV was applied to separate the sample. The capillary during electrophoresis was kept at 30 ° C. Detection and analysis were performed by an indirect UV detection method using a CAPI-3300 / 3200 system manufactured by Otsuka Electronics Co., Ltd. with a detection wavelength of 260 nm, and the amount of glucose was determined from the peak area.
上記のグルコース量の測定の結果を表1に示す。 The results of measurement of the glucose amount are shown in Table 1.
表1の結果から、糖アルコールと酵素を混合した場合は、ジャガイモ澱粉が酵素で分解されることで増加する内部のグルコース量が明らかに多く、糖アルコールによって酵素の浸透を促進させることが分かる。 From the results of Table 1, it can be seen that when sugar alcohol and enzyme are mixed, the amount of internal glucose that is increased when potato starch is decomposed by the enzyme is obviously large, and the sugar alcohol promotes the penetration of the enzyme.
<糖アルコールによる酵素の浸透促進効果と軟化効果の確認> <Confirmation of penetration and softening effect of enzyme by sugar alcohol>
[実施例2]
植物性食品素材としてジャガイモを使用した。実施例1と同様に用意したジャガイモを、アミラーゼを0.0356重量部、酵素製剤を4重量部、さらにマルチトールを20重量部、水を75.938重量部混合した糖アルコールと酵素液の混合液に、液と同重量の上記のジャガイモを浸漬させ、3℃の冷蔵庫で一晩保管して糖アルコールと酵素を含浸させた。その後、酵素失活は実施例1と同様、スチームコンベクションオーブンを使用して100℃で10分加熱した。
[Example 2]
Potato was used as a vegetable food material. Potato prepared in the same manner as in Example 1, 0.0356 parts by weight of amylase, 4 parts by weight of enzyme preparation, 20 parts by weight of maltitol, and 75.938 parts by weight of water mixed with sugar alcohol and enzyme solution The above-mentioned potato having the same weight as the liquid was immersed in the liquid and stored overnight in a refrigerator at 3 ° C. to impregnate the sugar alcohol and the enzyme. Thereafter, enzyme inactivation was carried out at 100 ° C. for 10 minutes using a steam convection oven as in Example 1.
以上の方法で軟化ジャガイモを作成したところ、軟化の前後でジャガイモの形状は保たれたままであった。一方、該軟化ジャガイモは、舌や歯茎などで押しつぶした際に抵抗のないかたさであり、押しつぶしたものは滑らかな食感であった。 When the softened potato was produced by the above method, the shape of the potato was kept before and after the softening. On the other hand, the softened potatoes had no resistance when crushed with a tongue or gums, and the crushed ones had a smooth texture.
また、ジャガイモ中のグルコース量について、外部と内部の比較から糖アルコールによる酵素の浸透性をさらに確認するために別の方法で測定を行った。グルコース量測定用のサンプルは、該軟化ジャガイモ(20mm角のさいころ形状)の周囲6面をすべて3mm厚さでスライスし、そのスライスを外周区とした。残ったジャガイモの周囲3mmを再度スライスしてこの部分は廃棄し、残った内側の8mm角を内部区とした。この外周区と内部区の試料を、それぞれ、以下に示すように、分析用上清として調整し、グルコース量の測定を行った。 Further, the amount of glucose in potato was measured by another method in order to further confirm the permeability of the enzyme by sugar alcohol from the comparison between outside and inside. In the sample for measuring the amount of glucose, all six sides of the softened potato (20 mm square dice shape) were sliced with a thickness of 3 mm, and the slice was used as an outer peripheral section. The remaining 3 mm around the remaining potato was sliced again and discarded, and the remaining 8 mm square was used as the inner section. As shown below, the samples in the outer peripheral section and the inner section were prepared as analytical supernatants, and the amount of glucose was measured.
グルコース量測定用の試料は同量の純水とともにポリ袋に入れて密封し、外から手で押しつぶしてペースト状にしたのち、ポリ袋のまま超音波洗浄機(シャープ社製 UT−205HS)内で60分間、超音波にかけた。その後、ポリ袋の内容物を回収し、濾過、遠心分離によって懸濁物質を除去した上清をHPLCによって測定した。 The sample for measuring the amount of glucose is placed in a plastic bag together with the same amount of pure water, sealed, crushed by hand from the outside and pasted into a paste, and then kept in a plastic bag with an ultrasonic cleaner (UT-205HS manufactured by Sharp Corporation). And sonicated for 60 minutes. Thereafter, the contents of the plastic bag were collected, and the supernatant from which suspended substances were removed by filtration and centrifugation was measured by HPLC.
HPLCは、昭和電工社製Shodex Asahipak NH2P−50 4Eカラム(ガードカラムとして同社Shodex Asahipak NH2P−50G 4A)を用い、アセトニトリル:水=80:20(v/v)を溶離液として40℃、1.0mL/minで分離し、RI検出機で検出したピーク面積から、外周区に対する内部区のグルコース量の比を計算した。 For HPLC, a Shodex Asahipak NH2P-50 4E column (manufactured by Shodex Asahipak NH2P-50G 4A as a guard column) manufactured by Showa Denko KK, acetonitrile: water = 80: 20 (v / v) as an eluent, 40 ° C., 1. The ratio of the amount of glucose in the inner section to the outer section was calculated from the peak area that was separated at 0 mL / min and detected by the RI detector.
[比較例2]
植物性食品素材としてジャガイモを使用し、糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を95.938重量部混合して酵素液を作成したこと以外は実施例2と同じ方法でジャガイモに酵素液を含浸させたのち、同じ方法で酵素失活させ、グルコース量の測定を行った。なお、処理したジャガイモのかたさは、舌や歯茎などで押しつぶした際に抵抗を感じるかたさであり、押しつぶしたものは固形感が残っていた。
[Comparative Example 2]
The same as Example 2 except that potato was used as a vegetable food material, and 95.938 parts by weight of water was mixed into the sugar alcohol / enzyme mixed solution instead of containing maltitol. After impregnating the potato with the enzyme solution by the method, the enzyme was inactivated by the same method, and the glucose amount was measured. The hardness of the treated potatoes was that they felt resistance when they were crushed with the tongue or gums, and the crushed ones remained solid.
実施例2及び比較例2の結果を表2に示す。 The results of Example 2 and Comparative Example 2 are shown in Table 2.
表2に示すグルコース量の外部と内部の比較の結果から、糖アルコールと酵素を混合した実施例2は、ジャガイモ澱粉が酵素で分解されることで増加するグルコース量について、外周区に対する内部区の比率を見ると、糖アルコールを使用せず酵素のみで軟化を行った比較例2と比べ大きい。酵素が中まで浸透せずに主に外周区ばかりで分解が行われている場合には、外周区の澱粉ばかりが分解され、内部区の澱粉が分解されないため、グルコースの内/外比は小さくなり、この比率が大きくなるほどに内部区まで酵素が浸透し、内部区の澱粉の分解が進んでいることになると考えられる。したがって、糖アルコールと酵素を混合した場合のほうが、酵素が内部まで含浸され、内外両方に作用した事を示していると考えられる。 From the results of comparison between the outside and inside of the glucose amount shown in Table 2, Example 2 in which the sugar alcohol and the enzyme were mixed, the glucose amount increased when the potato starch was decomposed by the enzyme, The ratio is larger than that of Comparative Example 2 in which the sugar alcohol is not used and softening is performed only with the enzyme. If the enzyme does not penetrate to the inside and the decomposition is mainly performed only in the outer peripheral section, only the starch in the outer peripheral section is decomposed and the starch in the inner section is not decomposed, so that the internal / external ratio of glucose is small. Thus, it is considered that as this ratio increases, the enzyme penetrates into the inner section, and the starch in the inner section is being decomposed. Therefore, it is considered that when the sugar alcohol and the enzyme are mixed, the enzyme is impregnated to the inside and acts both inside and outside.
また、同時にかたさを評価した結果も、糖アルコールと酵素を混合した場合のほうが、より軟化しており、酵素が糖アルコールで浸透促進され、軟化が促進されていることがわかる。 At the same time, the result of evaluating the hardness shows that the sugar alcohol and the enzyme are more softened, and the penetration of the enzyme with the sugar alcohol is promoted and the softening is promoted.
<各種植物性食品素材への適応例> <Examples of application to various vegetable food ingredients>
[実施例3]
植物性食品素材としてジャガイモを使用し、実施例1と同じように用意した。糖アルコールと酵素の混合液は、酵素製剤を4重量部、マルチトールを20重量部、水を76重量部混合して作成した。
[Example 3]
Potato was used as a vegetable food material and prepared in the same manner as in Example 1. The sugar alcohol / enzyme mixture was prepared by mixing 4 parts by weight of the enzyme preparation, 20 parts by weight of maltitol and 76 parts by weight of water.
作成した混合液に、液と同重量の上記のジャガイモを浸漬させ、実施例2と同じ方法で糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素を失活させた。 The potato having the same weight as the liquid was immersed in the prepared mixed liquid, impregnated with a mixed liquid of sugar alcohol and enzyme by the same method as in Example 2, and then the enzyme was inactivated by the same method.
以上の方法で軟化ジャガイモを作成したところ、軟化の前後でジャガイモの形状は保たれたままであった。そして、該軟化ジャガイモを、高さが15mmとなるように上部を切り落として、上述の方法でかたさの測定を行なった。 When the softened potato was produced by the above method, the shape of the potato was kept before and after the softening. The upper part of the softened potato was cut off so that the height was 15 mm, and the hardness was measured by the method described above.
[比較例3]
糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を96重量部混合して酵素液を作成したこと以外は実施例3と同じ方法でジャガイモに酵素液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 3]
After impregnating the potato with the enzyme solution in the same manner as in Example 3 except that the enzyme solution was prepared by mixing 96 parts by weight of water instead of containing maltitol in the sugar alcohol and enzyme mixture, The enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例4]
植物性食品素材としてキュウリを使用した。キュウリを健常者が食べやすい大きさとして約2cmの長さの小口切りにして、1分間下ゆでしたものを用意した。糖アルコールと酵素の混合液は、酵素製剤を3重量部、マルチトールを20重量部、水を77重量部混合して作成した。作成した混合液に浸漬させる方法は実施例3と同じ方法で行ったあと、スチームコンベクションオーブンを使用して100℃で3分加熱して酵素を失活させた。
[Example 4]
Cucumber was used as a vegetable food material. The cucumber was sized to be easy for a healthy person to eat and was cut into small slices of about 2 cm in length and then boiled for 1 minute. A mixed solution of sugar alcohol and enzyme was prepared by mixing 3 parts by weight of the enzyme preparation, 20 parts by weight of maltitol, and 77 parts by weight of water. The method of immersing in the prepared mixed solution was performed in the same manner as in Example 3, and then the enzyme was inactivated by heating at 100 ° C. for 3 minutes using a steam convection oven.
以上の方法で軟化キュウリを作成したところ、軟化の前後でキュウリの形状は保たれたままであった。そして、該軟化キュウリを、輪切り面に対して十字を入れる方向で四つ割にしたものを、輪切り面だった面が上向きとなるようにして、縦方向に置いたときに高さが15mmとなるように上部を切り落として、立てるようにして測定セルに入れ、上述の方法でかたさの測定を行なった。 When the softened cucumber was prepared by the above method, the shape of the cucumber was kept before and after the softening. Then, when the softened cucumber is divided into four parts in the direction of inserting a cross with respect to the cut surface, the height of the cut cucumber is 15 mm when placed in the vertical direction so that the surface that was the cut surface faces upward. The upper part was cut off and placed in a measuring cell in a standing manner, and the hardness was measured by the method described above.
[比較例4]
糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を97重量部混合して酵素液を作成したこと以外は実施例4と同じ方法でキュウリに酵素液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 4]
After impregnating the cucumber with the enzyme solution in the same manner as in Example 4, except that the mixture of sugar alcohol and enzyme did not contain maltitol, instead of containing 97 parts by weight of water to prepare the enzyme solution, The enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例5]
植物性食品素材として白菜を使用した。白菜の白く厚みのある芯の部分を約3cm四方に切り、30秒間下ゆでしたものを用意した。実施例4と同じ糖アルコールと酵素の混合液を作製し、作成した混合液に、液と同重量の上記の白菜芯を浸漬させ40℃で3時間保管し、糖アルコールと酵素の混合液を含浸させたのち、スチームコンベクションオーブンを使用して100℃で5分加熱して酵素を失活させた。
[Example 5]
Chinese cabbage was used as a vegetable food material. A white and thick core portion of Chinese cabbage was cut into 3 cm squares and prepared for 30 seconds. The same mixture of sugar alcohol and enzyme as in Example 4 was prepared, and the Chinese cabbage core of the same weight as the liquid was immersed in the prepared mixture and stored at 40 ° C. for 3 hours. After impregnation, the enzyme was inactivated by heating at 100 ° C. for 5 minutes using a steam convection oven.
以上の方法で軟化白菜芯を作成したところ、軟化の前後で白菜芯の形状は保たれたままであった。そして、該軟化白菜芯を用いて、上述の方法でかたさの測定を行なった。なお上述のとおり、試料は直接、試料台に置き、試料高さの66.67%まで圧縮するよう測定した。 When the softened Chinese cabbage core was prepared by the above method, the shape of the Chinese cabbage core was kept before and after the softening. And the hardness was measured by the above-mentioned method using this softened Chinese cabbage core. As described above, the sample was directly placed on the sample stage and measured to compress to 66.67% of the sample height.
[比較例5]
糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を97重量部混合して酵素液を作成したこと以外は実施例5と同じ方法で白菜の芯に酵素液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 5]
Instead of containing maltitol in the sugar alcohol / enzyme mixture, 97 parts by weight of water was mixed to prepare the enzyme solution, and the Chinese cabbage core was impregnated with the enzyme solution in the same manner as in Example 5. Thereafter, the enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例6]
植物性食品素材として白菜を使用した。白菜の葉の部分を約3cm幅に切り、30秒間下ゆでしたものを用意した。糖アルコールと酵素の混合液および混合液の含浸および失活させる方法は、実施例5と同じ方法として軟化白菜葉を作成したところ軟化の前後で白菜葉の形状は保たれたままであった。そして、該軟化白菜葉を用いて上述の方法でかたさの測定を行なった。
[Example 6]
Chinese cabbage was used as a vegetable food material. A Chinese cabbage leaf portion was cut into a width of about 3 cm and boiled for 30 seconds. The mixed solution of sugar alcohol and enzyme and the method of impregnating and deactivating the mixed solution were the same as in Example 5. When softened Chinese cabbage leaves were prepared, the shape of the Chinese cabbage leaves was maintained before and after softening. And hardness was measured by the above-mentioned method using this softened Chinese cabbage leaf.
[比較例6]
糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を97重量部混合して酵素液を作成したこと以外は実施例5と同じ方法で白菜の葉に酵素液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 6]
Instead of containing maltitol in the sugar alcohol / enzyme mixed solution, 97 parts by weight of water was mixed to prepare the enzyme solution, and the Chinese cabbage leaf was impregnated with the enzyme solution in the same manner as in Example 5. Thereafter, the enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例7]
植物性食品素材として大根を使用した。大根を太さが約1cmで長さが約2cmの拍子切りにして、3分間下ゆでしたものを用意した。糖アルコールと酵素の混合液は、酵素製剤を1重量部、マルチトールを20重量部、水を79重量部混合して作成した。作成した混合液に浸漬させる方法は実施例5と同じ方法で行ったあと、酵素の失活は実施例1と同じ方法で行なった。
[Example 7]
Radish was used as a vegetable food material. A daikon radish with a thickness of about 1 cm and a length of about 2 cm was prepared and boiled for 3 minutes. A mixture of sugar alcohol and enzyme was prepared by mixing 1 part by weight of the enzyme preparation, 20 parts by weight of maltitol and 79 parts by weight of water. The method of immersing in the prepared mixed solution was performed in the same manner as in Example 5, and then the enzyme was deactivated in the same manner as in Example 1.
以上の方法で軟化大根を作成したところ、軟化の前後で大根の形状は保たれたままであった。そして、該軟化大根を、拍子切りの長辺が高さ方向となるようにして、縦方向に置いたときに高さが15mmとなるように上部を切り落として、立てるようにして測定セルに入れ、上述の方法でかたさの測定を行なった。 When the softened radish was prepared by the above method, the shape of the radish was kept before and after the softening. Then, the softened radish is placed in the measuring cell so that the long side of the beat is cut in the height direction, and the upper part is cut off so that the height is 15 mm when placed in the vertical direction. The hardness was measured by the method described above.
[比較例7−1]
糖アルコールと酵素の混合液に、マルチトールを含まない代わりに、水を99重量部混合して酵素液を作成したこと以外は実施例7と同じ方法で大根に酵素液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 7-1]
After impregnating the radish with the enzyme solution in the same manner as in Example 7 except that the enzyme solution was prepared by mixing 99 parts by weight of water instead of containing maltitol in the sugar alcohol and enzyme mixture, The enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[比較例7−2]
糖アルコールと酵素の混合液に含まれるマルチトールを砂糖に換えた以外は実施例7と同じ方法で大根に砂糖と酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行なった。
[Comparative Example 7-2]
The radish was impregnated with a mixture of sugar and enzyme in the same manner as in Example 7 except that the maltitol contained in the mixture of sugar alcohol and enzyme was replaced with sugar. The hardness was measured by this method.
実施例3〜7及び比較例3〜6、7−1、7−2の結果を表3に示す。 Table 3 shows the results of Examples 3 to 7 and Comparative Examples 3 to 6, 7-1 and 7-2.
[実施例8]
植物性食品素材としてノースイ社製の冷凍インゲンを使用し、20〜30mm長さにカットして用意した。糖アルコールと酵素の混合液は、酵素製剤を1.5重量部、ソルビトールを20重量部、水を78.5重量部混合して作成した。作成した混合液に浸漬させる方法は実施例2同じ方法で行ったあと、酵素失活は実施例5と同様に行った。なお、ソルビトールは三菱商事フードテック社より販売されているLTS−P50Mを使用した。以降もソルビトールを使用する場合は同様である。
[Example 8]
As a vegetable food material, a frozen kidney beans manufactured by Northoy Co., Ltd. was used, and cut into 20 to 30 mm length to prepare. A mixture of sugar alcohol and enzyme was prepared by mixing 1.5 parts by weight of the enzyme preparation, 20 parts by weight of sorbitol, and 78.5 parts by weight of water. The method of immersing in the prepared mixed solution was performed in the same manner as in Example 2, and then enzyme deactivation was performed in the same manner as in Example 5. The sorbitol used was LTS-P50M sold by Mitsubishi Corporation Foodtech. Thereafter, the same applies when sorbitol is used.
以上の方法で、軟化インゲンを作成したところ、軟化の前後でインゲンの形状は保たれていた。そして、該軟化インゲンを、縦方向に置いたときに高さが15mmとなるように上部を切り落として、サヤインゲンを立てる方向となるようにしてセルに入れ、上述の方法でかたさ測定を行った。 When softened green beans were prepared by the above method, the shape of green beans was maintained before and after softening. Then, the soft beans were cut off at the top so that the height was 15 mm when placed in the vertical direction, placed in a cell so that the green beans were raised, and the hardness was measured by the method described above.
[比較例8]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を98.5重量部混合して酵素液を作成したこと以外は実施例8と同じ方法で冷凍インゲンに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 8]
The enzyme solution was immersed in frozen kidney beans in the same manner as in Example 8 except that the enzyme solution was prepared by mixing 98.5 parts by weight of water instead of containing sorbitol in the sugar alcohol and enzyme mixture. Thereafter, the enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例9]
植物性食品素材としてレンコンを使用し、10mmの厚みの輪切りスライスにして、2分間下ゆでしたものを用意した。糖アルコールと酵素の混合液は、酵素製剤を2重量部、ソルビトールを20重量部、水を78重量部混合して作成した。作成した混合液に浸漬させる方法および酵素を失活させる方法は実施例2と同じ方法で行った。
[Example 9]
A lotus root was used as a vegetable food material, and sliced slices having a thickness of 10 mm were prepared for 2 minutes. A mixture of sugar alcohol and enzyme was prepared by mixing 2 parts by weight of the enzyme preparation, 20 parts by weight of sorbitol, and 78 parts by weight of water. The method of immersing in the prepared mixed solution and the method of deactivating the enzyme were performed in the same manner as in Example 2.
以上の方法で、軟化レンコンを作成したところ、軟化の前後でレンコンの形状は保たれていた。かたさ測定は、上述の方法で行った。ただし、測定時には、測定台に直接置いて、輪切り面を測定台に接地させ、レンコン穴の影響の出ないよう、レンコン中心部で測定を行い、実施例5と同様、試料高さの66.67%まで圧縮するよう測定した。 When the softened lotus root was prepared by the above method, the shape of the lotus root was maintained before and after the softening. The hardness measurement was performed by the method described above. However, at the time of measurement, the sample was placed directly on the measurement table, the cut surface was grounded to the measurement table, and measurement was performed at the center of the lotus root so as not to be affected by the lotus hole. Measured to compress to 67%.
[比較例9]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を98重量部混合して酵素液を作成したこと以外は実施例9と同じ方法でレンコンに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 9]
Instead of not containing sorbitol in the mixture of sugar alcohol and enzyme, the enzyme solution was immersed in lotus root in the same manner as in Example 9 except that 98 parts by weight of water was mixed to prepare the enzyme solution. The enzyme was inactivated by the method, and the hardness was measured by the method described above.
[実施例10]
植物性食品素材としてブロッコリーを使用し、小房に分けて2分間下ゆでした。茎の部分を厚み15mmにスライスし、さらにいちょう切りにして用意した。実施例4と同じ糖アルコールと酵素の混合液を作成し、作成した混合液に、液と同重量の上記のブロッコリーを浸漬させた。作成した混合液に浸漬させる方法および酵素を失活させる方法は実施例2と同じ方法で行った。
[Example 10]
Broccoli was used as a vegetable food material and it was boiled for 2 minutes in small bunches. The stem portion was sliced to a thickness of 15 mm and further cut into icicles. The same mixture of sugar alcohol and enzyme as in Example 4 was prepared, and the above broccoli having the same weight as the solution was immersed in the prepared mixture. The method of immersing in the prepared mixed solution and the method of deactivating the enzyme were performed in the same manner as in Example 2.
以上の方法で、軟化ブロッコリーを作成したところ、軟化の前後での形状は保たれていた。かたさ測定は、茎の部分について上述の方法で行った。測定時には、実施例5と同様に、測定台に直接置き、試料高さの66.67%まで圧縮するよう測定した。 When softened broccoli was created by the above method, the shape before and after softening was maintained. Hardness measurement was performed by the above-mentioned method about the stem part. At the time of measurement, in the same manner as in Example 5, the sample was placed directly on a measurement table and measured to compress to 66.67% of the sample height.
[比較例10]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を97重量部混合して酵素液を作成したこと以外は実施例10と同じ方法でブロッコリーに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 10]
Instead of not containing sorbitol in the mixture of sugar alcohol and enzyme, the enzyme solution was immersed in broccoli in the same manner as in Example 10 except that 97 parts by weight of water was mixed to prepare the enzyme solution. The enzyme was inactivated by the method, and the hardness was measured by the method described above.
[実施例11]
植物性食品素材としてエリンギを使用し、軸の部分を10mmの厚みの輪切りスライスにした生のままのものを用意した。糖アルコールと酵素の混合液は、酵素製剤を1.5重量部、ソルビトールを20重量部、水を78.5重量部混合して作成した。作成した混合液に浸漬させる方法および酵素を失活させる方法は実施例2と同じ方法で行った。
[Example 11]
A raw material was prepared by using eringi as a vegetable food material and having a shaft sliced into a sliced slice having a thickness of 10 mm. A mixture of sugar alcohol and enzyme was prepared by mixing 1.5 parts by weight of the enzyme preparation, 20 parts by weight of sorbitol, and 78.5 parts by weight of water. The method of immersing in the prepared mixed solution and the method of deactivating the enzyme were performed in the same manner as in Example 2.
以上の方法で、軟化エリンギを作成したところ、軟化の前後での形状は保たれていた。かたさ測定は、上述の方法で行った。測定時には、実施例5と同様に、測定台に直接置き、試料高さの66.67%まで圧縮するよう測定した。 When the softened eringi was prepared by the above method, the shape before and after the softening was maintained. The hardness measurement was performed by the method described above. At the time of measurement, in the same manner as in Example 5, the sample was placed directly on a measurement table and measured to compress to 66.67% of the sample height.
[比較例11]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を98.5重量部混合して酵素液を作成したこと以外は実施例11と同じ方法でエリンギに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 11]
After immersing the enzyme solution in Eringi in the same manner as in Example 11 except that the enzyme solution was prepared by mixing 98.5 parts by weight of water instead of sorbitol in the sugar alcohol and enzyme mixture. The enzyme was inactivated by the same method, and the hardness was measured by the method described above.
[実施例12]
植物性食品素材としてリンゴを使用し、20mm角に切り、3分間下ゆでしたものを用意した。糖アルコールと酵素の混合液および、浸漬、失活の方法は実施例9と同じ方法で行った。
[Example 12]
An apple was used as a vegetable food material, cut into 20 mm squares, and boiled for 3 minutes. The mixed solution of sugar alcohol and enzyme, and the dipping and deactivation methods were the same as in Example 9.
以上の方法で、軟化リンゴを作成したところ、軟化の前後での形状は保たれていた。かたさ測定は、実施例5と同様に、測定台に直接置き、試料高さの66.67%まで圧縮するよう測定した。 When the softened apple was created by the above method, the shape before and after softening was maintained. In the same manner as in Example 5, the hardness was measured by placing it directly on the measuring table and compressing it to 66.67% of the sample height.
[比較例12]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を98重量部混合して酵素液を作成したこと以外は実施例12と同じ方法でリンゴに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 12]
Instead of not containing sorbitol in the mixture of sugar alcohol and enzyme, the enzyme solution was immersed in the apple in the same manner as in Example 12 except that 98 parts by weight of water was mixed to prepare the enzyme solution. The enzyme was inactivated by the method, and the hardness was measured by the method described above.
実施例8〜12及び比較例8〜12の結果を表4に示す。 Table 4 shows the results of Examples 8 to 12 and Comparative Examples 8 to 12.
[実施例13]
植物性食品素材として乾燥コンブを使用した。糖アルコールと酵素の混合液は、酵素製剤を5重量部、ソルビトールを80重量部、水を315重量部混合して作成した。作成した糖アルコールと酵素の混合液に、液400重量部に対し100重量部の上記コンブを浸漬させ25℃で4時間保管し、糖アルコールと酵素の混合液を含浸させたのち、スチームコンベクションオーブンを使用して95℃で10分加熱して酵素を失活させた。
[Example 13]
Dried kombu was used as a vegetable food material. A mixture of sugar alcohol and enzyme was prepared by mixing 5 parts by weight of the enzyme preparation, 80 parts by weight of sorbitol, and 315 parts by weight of water. In the prepared mixture of sugar alcohol and enzyme, 100 parts by weight of the above-mentioned combination is immersed in 400 parts by weight of the liquid, stored at 25 ° C. for 4 hours, impregnated with the mixture of sugar alcohol and enzyme, and then a steam convection oven. Was used to inactivate the enzyme by heating at 95 ° C. for 10 minutes.
以上の方法で、軟化コンブを作成したところ、コンブが水戻りして大きくはなったが、崩れたりせず、軟化の前後での形状は保たれていた。かたさの測定は、他の実施例と同様にテクスチャー測定で行おうとしたところ、コンブの薄さとぬるぬるとした表面のために、試料の圧縮でかたさを測ることができなかった。そのため、同じ測定機器を用いて、上述の方法で破断強度測定モードによりかたさ測定を行った。 When the softened comb was made by the above method, the comb returned to water and became larger, but it did not collapse and the shape before and after softening was maintained. Hardness was measured by texture measurement in the same manner as in the other examples, but due to the thinness of the comb and the slimy surface, the hardness could not be measured by compressing the sample. Therefore, the hardness was measured in the breaking strength measurement mode by the above-described method using the same measuring device.
そして、破断強度の測定によるかたさ測定を利用して、以下のように軟化度を求めた。 And the degree of softening was calculated | required as follows using the hardness measurement by the measurement of breaking strength.
軟化度は、酵素と、糖または糖アルコールを混合しない条件(比較例13)で酵素液を含浸させた軟化コンブの破断強度を100として、糖アルコールと酵素の混合液を含浸させた軟化コンブの破断強度をXであらわし、どの程度軟らかくなっているかを破断強度(応力)から、軟化度=100−Xの式で求めた。 The softening degree of the softened comb impregnated with the mixed solution of sugar alcohol and enzyme, with the breaking strength of the softened comb impregnated with the enzyme solution under the condition where the enzyme and sugar or sugar alcohol are not mixed (Comparative Example 13) as 100 The breaking strength is represented by X, and the degree of softening was determined from the breaking strength (stress) by the formula of softening degree = 100−X.
[比較例13]
糖アルコールと酵素の混合液に、ソルビトールを含まない代わりに、水を395重量部混合して酵素液を作成したこと以外は実施例13と同じ方法で乾燥コンブに酵素液を浸漬させたのち、同じ方法で酵素失活させ、上述の方法でかたさ測定を行った。
[Comparative Example 13]
Instead of not containing sorbitol in the sugar alcohol and enzyme mixture, 395 parts by weight of water was mixed to prepare the enzyme solution, and after immersing the enzyme solution in the dry comb in the same manner as in Example 13, The enzyme was inactivated by the same method, and the hardness was measured by the method described above.
実施例13及び比較例13の結果を表5に示す。 The results of Example 13 and Comparative Example 13 are shown in Table 5.
表3から表5の結果から、糖アルコールと酵素を混合することで、酵素のみの場合や、酵素と砂糖を混合する場合よりも、効果的に植物性食品素材を摂取しやすく軟化できることがわかる。 From the results of Tables 3 to 5, it can be seen that mixing the sugar alcohol and the enzyme can effectively soften the vegetable food material more easily than the case of using only the enzyme or the case of mixing the enzyme and sugar. .
<各種糖アルコールおよび糖類での効果> <Effects with various sugar alcohols and sugars>
[実施例14−1]
植物性食品素材として冷凍ゴボウを使用した。冷凍ゴボウは株式会社米川商事社製の、1cmから3cm程度の丸太状にぶつ切りされたもののうち約2cm以上のものを選んで、解凍したのち表面に付着した水分をザルで切った状態で用意した。糖アルコールと酵素の混合液は、酵素製剤を5重量部、マルチトールを20重量部、水を75重量部混合して作成した。作成した混合液に浸漬させる方法は実施例5と同じ方法で行った。酵素失活はスチームコンベクションオーブンを使用して150℃で10分加熱した。
[Example 14-1]
Frozen burdock was used as a vegetable food material. Frozen burdock, made by Yonekawa Shoji Co., Ltd., was prepared by cutting about 1 cm to 3 cm of a log and about 2 cm or more, and thawing the water adhering to the surface after thawing. . A mixed solution of sugar alcohol and enzyme was prepared by mixing 5 parts by weight of the enzyme preparation, 20 parts by weight of maltitol, and 75 parts by weight of water. The method of immersing in the prepared mixed solution was performed in the same manner as in Example 5. The enzyme inactivation was heated at 150 ° C. for 10 minutes using a steam convection oven.
以上の方法で軟化ぶつ切りゴボウを作成したところ、軟化の前後でゴボウの形状は保たれたままであった。そして、該軟化ぶつ切りゴボウを、輪切り面に対して十字を入れる方向で四つ割にしたものを、輪切り面だった面が上向となるようにして、縦方向に置いたときに高さが15mmとなるように上部を切り落として、立てるようにして測定セルに入れ、上述の方法でかたさの測定を行なった。 When the softened chopped burdock was created by the above method, the shape of the burdock was kept before and after the softening. Then, when the softened chopped burdock is divided into four parts in the direction of inserting a cross with respect to the cut surface, the height of the softened burdock burdock when it is placed in the vertical direction with the surface that was the cut surface facing upwards The upper part was cut off so as to be 15 mm, and it was placed upright into the measurement cell, and the hardness was measured by the method described above.
[実施例14−2]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールをソルビトールに換えた以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ軟化ゴボウを作成したところ、軟化の前後でゴボウの形状は保たれたままであった。そして、該軟化ゴボウを用いて実施例14−1と同じ方法でかたさ測定を行なった。
[Example 14-2]
The burdock was impregnated with a mixed solution of sugar alcohol and enzyme in the same manner as in Example 14-1, except that frozen burdock was used as a vegetable food material and maltitol contained in the mixed solution of sugar alcohol and enzyme was replaced with sorbitol. Then, the enzyme was inactivated by the same method to produce a softened burdock, and the shape of the burdock was kept before and after the softening. And hardness was measured by the same method as Example 14-1 using this softened burdock.
[実施例14−3]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールを還元澱粉糖化物に換えた以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。軟化の前後でゴボウの形状は保たれたままであった。還元澱粉糖化物は三菱商事フードテック社製のアマミールシロップを使用した。アマミールシロップに含まれるソルビトールおよびマルチトールは、含有糖アルコールの70%以上である。
[Example 14-3]
A mixture of sugar alcohol and enzyme was added to burdock in the same manner as in Example 14-1, except that frozen burdock was used as a vegetable food material and maltitol contained in the mixture of sugar alcohol and enzyme was replaced with reduced starch saccharified product. After impregnating the solution, the enzyme was inactivated by the same method, and the hardness was measured by the same method. The shape of the burdock remained the same before and after softening. As the reduced starch saccharified product, Amami syrup manufactured by Mitsubishi Corporation Foodtech was used. Sorbitol and maltitol contained in the amyl meal syrup are 70% or more of the contained sugar alcohol.
[実施例14−4]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールを還元澱粉糖化物に換えた以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。軟化の前後でゴボウの形状は保たれたままであった。還元澱粉糖化物は三菱商事フードテック社製のPO−20シロップを使用した。PO−20シロップに含まれるソルビトールおよびマルチトールは、含有糖アルコールの10%以上である。
[Example 14-4]
A mixture of sugar alcohol and enzyme was added to burdock in the same manner as in Example 14-1, except that frozen burdock was used as a vegetable food material and maltitol contained in the mixture of sugar alcohol and enzyme was replaced with reduced starch saccharified product. After impregnating the solution, the enzyme was inactivated by the same method, and the hardness was measured by the same method. The shape of the burdock remained the same before and after softening. The reduced starch saccharified product used was PO-20 syrup manufactured by Mitsubishi Corporation Foodtech. Sorbitol and maltitol contained in the PO-20 syrup is 10% or more of the contained sugar alcohol.
[比較例14−1]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液にマルチトールを含まない代わりに、水を95重量部混合して酵素液を作成したこと以外は実施例14−1と同じ方法でゴボウに酵素液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Comparative Example 14-1]
The same as Example 14-1, except that frozen burdock was used as a vegetable food material and 95 parts by weight of water was mixed instead of containing maltitol in the sugar alcohol and enzyme mixture. After impregnating the burdock with the enzyme solution by the method, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
[比較例14−2]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールを砂糖に換えた以外は実施例14−1と同じ方法でゴボウに砂糖と酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Comparative Example 14-2]
A burdock is impregnated with a mixed solution of sugar and enzyme in the same manner as in Example 14-1, except that frozen burdock is used as a vegetable food material and maltitol contained in the mixed solution of sugar alcohol and enzyme is replaced with sugar. After that, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
[比較例14−3]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールをトレハロースに換えた以外は実施例14−1と同じ方法でゴボウにトレハロースと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Comparative Example 14-3]
A burdock was impregnated with a mixed solution of trehalose and an enzyme in the same manner as in Example 14-1, except that frozen burdock was used as a vegetable food material and maltitol contained in the mixed solution of sugar alcohol and enzyme was replaced with trehalose. After that, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
実施例14−1から14−4及び比較例14−1から14−3の結果を表6に示す。 Table 6 shows the results of Examples 14-1 to 14-4 and Comparative Examples 14-1 to 14-3.
表6の結果から、糖アルコールと酵素を混合することで、酵素と一般的な糖類を混合する場合よりも、効果的に植物性食品素材を摂取しやすく軟化できることがわかる。 From the results of Table 6, it can be seen that mixing the sugar alcohol and the enzyme can effectively ingest and soften the vegetable food material more effectively than mixing the enzyme and a general sugar.
<効果的な糖アルコールの量> <Effective amount of sugar alcohol>
[実施例15−1]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液を、酵素製剤を5重量部、マルチトールを40重量部、水を55重量部とした以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、軟化ゴボウを作成したところ、糖アルコールの量が多くなっても軟化の前後でゴボウの形状は保たれたままであった。そして、該軟化ゴボウを用いて実施例14−1と同じ方法でかたさ測定を行なった。
[Example 15-1]
The same as Example 14-1, except that frozen burdock was used as the vegetable food material, and the sugar alcohol and enzyme mixture was 5 parts by weight of the enzyme preparation, 40 parts by weight of maltitol, and 55 parts by weight of water. After impregnating burdock with a mixture of sugar alcohol and enzyme, the enzyme was deactivated by the same method to create a softened burdock. Even if the amount of sugar alcohol increased, the shape of burdock was maintained before and after softening. It remained leaning. And hardness was measured by the same method as Example 14-1 using this softened burdock.
[実施例15−2]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールをソルビトールに換え、糖アルコールと酵素の混合液を、酵素製剤を5重量部、ソルビトールを40重量部、水を55重量部とした以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。軟化の前後でゴボウの形状は保たれたままであった。
[Example 15-2]
Use frozen burdock as a vegetable food material, replace maltitol contained in the sugar alcohol and enzyme mixture with sorbitol, 5 parts by weight of the enzyme preparation, 40 parts by weight of sorbitol, Except that water was changed to 55 parts by weight, burdock was impregnated with a mixed solution of sugar alcohol and enzyme in the same manner as in Example 14-1, and then the enzyme was inactivated by the same method, and the hardness was measured by the same method. The shape of the burdock remained the same before and after softening.
[比較例15−1]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液を、酵素製剤を5重量部、マルチトールを10重量部、水を85重量部とした以外は実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Comparative Example 15-1]
The same as Example 14-1, except that frozen burdock was used as a vegetable food material, the sugar alcohol and enzyme mixture was 5 parts by weight of enzyme preparation, 10 parts by weight of maltitol, and 85 parts by weight of water. After impregnating burdock with a mixture of sugar alcohol and enzyme by the method, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
[比較例15−2]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液に含まれるマルチトールをソルビトールに換え、ソルビトールと酵素の混合液を、酵素製剤を5重量部、ソルビトールを10重量部、水を85重量部とした以外は実施例14−1と同じ方法でゴボウに酵素液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Comparative Example 15-2]
Using frozen burdock as a vegetable food material, replacing maltitol contained in the sugar alcohol and enzyme mixture with sorbitol, 5 parts by weight of the enzyme preparation, 10 parts by weight of sorbitol, and 10 parts by weight of water The burdock was impregnated with the enzyme solution by the same method as in Example 14-1, except that the amount of was 85 parts by weight. Then, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
実施例15−1から15−2及び比較例15−1から15−2の結果を表7に示す。 Table 7 shows the results of Examples 15-1 to 15-2 and Comparative Examples 15-1 to 15-2.
表7の結果から、糖アルコールと酵素を混合する際は、糖アルコールの量が15重量部未満では酵素の浸透促進効果が少ないことがわかる。また、糖アルコールの量が40重量部においては高い酵素浸透促進効果を得られ、効果的に植物性食品素材を摂取しやすく軟化できることがわかる。 From the results of Table 7, it can be seen that when the sugar alcohol and the enzyme are mixed, if the amount of the sugar alcohol is less than 15 parts by weight, the enzyme penetration promoting effect is small. It can also be seen that when the amount of sugar alcohol is 40 parts by weight, a high enzyme permeation promoting effect can be obtained and the vegetable food material can be effectively ingested and softened effectively.
<表面処理を併用した場合の糖アルコールの効果> <Effects of sugar alcohol when combined with surface treatment>
[実施例16]
植物性食品素材として冷凍ゴボウを使用し、解凍後、含浸を促進させるための処理として、ぶつ切りゴボウ表面から深さ10mm程度に、針を刺すテンダライズ処理をした。その後、実施例14−1と同じ糖アルコールと酵素の混合液を用いて浸漬させたが、浸漬開始時に糖アルコールと酵素の混合液とゴボウを容器内でよく振り混ぜるタンブリング処理をしてから、保管した。上記のテンダライズ処理およびタンブリング処理を行った以外は、実施例14−1と同じ方法でゴボウに糖アルコールと酵素の混合液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行なった。
[Example 16]
Frozen burdock was used as a vegetable food material, and after thawing, as a process for promoting impregnation, a tenderizing process was performed in which a needle was inserted to a depth of about 10 mm from the chopped burdock surface. Then, it was immersed using the same sugar alcohol and enzyme mixture as in Example 14-1, but after the tumbling treatment in which the sugar alcohol and enzyme mixture and burdock were well shaken in the container at the start of immersion, Stored. Except for performing the above-described tenderization treatment and tumbling treatment, after impregnating a burdock with a mixture of sugar alcohol and enzyme in the same manner as in Example 14-1, the enzyme was deactivated in the same manner, and the hardness was measured in the same manner. Was done.
実施例16の結果を表8に示す。 The results of Example 16 are shown in Table 8.
表8の結果から、含浸を促進させるための処理として、テンダライズ処理・タンブリング処理を行い、糖アルコールと酵素の混合液に浸漬させると、含浸を促進させるための処理を行わず浸漬させる場合に比べ、さらに酵素の浸透促進効果が得られ、より効果的に植物性食品素材を軟化できることがわかる。 From the results of Table 8, as a treatment for promoting impregnation, a tenderizing treatment and a tumbling treatment are performed, and when immersed in a mixed solution of sugar alcohol and enzyme, compared to a case where immersion is performed without performing a treatment for promoting impregnation. Further, it can be seen that the effect of promoting the penetration of the enzyme is obtained, and the vegetable food material can be softened more effectively.
<糖アルコールと酵素の混合粉末による効果> <Effect of mixed powder of sugar alcohol and enzyme>
[実施例17]
植物性食品素材として冷凍ゴボウを使用した。冷凍ゴボウは実施例14−1と同じものを使用した。解凍したのち、表面に付着した水分をザルで切った状態で用意した。糖アルコールと酵素は、酵素製剤を5重量部、マルチトールを20重量部、混合して混合粉末を作成し、用意したゴボウ100重量部と混合し3℃の冷蔵庫で一晩保管して糖アルコールと酵素を含浸させた。酵素失活は、加熱効率をあげるため、水を100重量部添加し、スチームコンベクションオーブンを使用して150℃で10分加熱した。
[Example 17]
Frozen burdock was used as a vegetable food material. The same frozen burdock as Example 14-1 was used. After thawing, the water adhering to the surface was prepared in a state of being cut with a colander. For sugar alcohol and enzyme, 5 parts by weight of enzyme preparation and 20 parts by weight of maltitol are mixed to prepare a mixed powder, mixed with 100 parts by weight of prepared burdock, and stored overnight in a refrigerator at 3 ° C. And impregnated with enzyme. In order to increase the heating efficiency, the enzyme deactivation was performed by adding 100 parts by weight of water and heating at 150 ° C. for 10 minutes using a steam convection oven.
以上の方法で軟化ぶつ切りゴボウを作成したところ、軟化の前後でゴボウの形状は保たれたままであった。そして、該軟化ぶつ切りゴボウを、輪切り面に対して十字を入れる方向で四つ割にしたものを、輪切り面だった面が上向となるようにして、実施例14−1と同じ方法でかたさ測定を行った。 When the softened chopped burdock was created by the above method, the shape of the burdock was kept before and after the softening. Then, the softened chopped burdock was divided into four parts in the direction of inserting a cross with respect to the cut surface, and the surface that was the cut surface was turned upward in the same manner as in Example 14-1. Measurements were made.
[比較例17]
植物性食品素材として冷凍ゴボウを使用し、酵素製剤を糖アルコールと混合せず、粉末状の製剤のまま5重量部を、実施例17と同様の方法で準備したゴボウと混合した。糖アルコールを含まないこと以外は実施例17と同様の方法で、酵素の含浸と失活を行い、軟化ゴボウを作成し、同じ方法でかたさ測定を行った。
[Comparative Example 17]
Frozen burdock was used as a vegetable food material, the enzyme preparation was not mixed with sugar alcohol, and 5 parts by weight of the powdered preparation was mixed with burdock prepared in the same manner as in Example 17. Enzyme impregnation and deactivation were performed in the same manner as in Example 17 except that sugar alcohol was not included, softened burdock was prepared, and the hardness was measured by the same method.
実施例17及び比較例17の結果を表9に示す。 Table 9 shows the results of Example 17 and Comparative Example 17.
表9の結果から、粉末状でも、糖アルコールと酵素を混合することで、効果的に植物性食品素材を摂取しやすく軟化できることがわかる。 From the results in Table 9, it can be seen that, even in powder form, the vegetable food material can be effectively ingested and softened effectively by mixing the sugar alcohol and the enzyme.
<浸漬以外の酵素含浸の効果> <Effect of enzyme impregnation other than immersion>
[実施例18]
植物性食品素材として冷凍ゴボウを使用した。冷凍ゴボウは実施例14−1と同じものを使用した。解凍したのち、表面に付着した水分をザルで切った状態で用意した。糖アルコールと酵素の混合液は、実施例14−1においてマルチトールをソルビトールに変更した以外は同様の方法で作成した。
[Example 18]
Frozen burdock was used as a vegetable food material. The same frozen burdock as Example 14-1 was used. After thawing, the water adhering to the surface was prepared in a state of being cut with a colander. A mixed solution of sugar alcohol and enzyme was prepared in the same manner except that maltitol was changed to sorbitol in Example 14-1.
用意したゴボウに対し、注射器を用いて糖アルコールと酵素の混合液を、1箇所につき0.2mLずつ、ぶつ切りにしてあるゴボウ1切れに対し、両端の切断面にそれぞれ5箇所ずつ、円柱の側面にあたる周囲の面にまんべんなく12箇所、計22箇所に注入し、用意したゴボウのぶつ切り全個体に注入した。この結果、糖アルコールと酵素の混合液は、総量で、ゴボウ150重量部に対し100重量部であった。糖アルコールと酵素の混合液の注入後、3℃の冷蔵庫で一晩保管して糖アルコールと酵素を含浸させた。酵素失活は、実施例14−1と同じ方法で行った。 For the prepared burdock, use a syringe to mix the sugar alcohol and enzyme mixture into 0.2 mL of each part, and for each piece of burdock that has been cut into 5 pieces, 5 each on the cut surfaces at both ends, the side of the cylinder It was injected evenly into the surrounding surface corresponding to 12 locations in total, 22 locations, and injected into all the burdock chopped individuals prepared. As a result, the total amount of the mixture of sugar alcohol and enzyme was 100 parts by weight with respect to 150 parts by weight of burdock. After injecting the mixture of sugar alcohol and enzyme, the mixture was stored overnight in a refrigerator at 3 ° C. and impregnated with sugar alcohol and enzyme. Enzyme deactivation was performed in the same manner as in Example 14-1.
以上の方法で軟化ぶつ切りゴボウを作成したところ、軟化の前後でゴボウの形状は保たれたままであった。そして、該軟化ぶつ切りゴボウを、実施例14−1と同じ方法でかたさ測定を行った。 When the softened chopped burdock was created by the above method, the shape of the burdock was kept before and after the softening. The softened chopped burdock was measured for hardness in the same manner as in Example 14-1.
[比較例18]
植物性食品素材として冷凍ゴボウを使用し、糖アルコールと酵素の混合液にソルビトールを含まない代わりに、水を95重量部混合して酵素液を作成したこと以外は実施例18と同じ方法でゴボウに酵素液を含浸させたのち、同じ方法で酵素失活させ、同じ方法でかたさ測定を行った。
[Comparative Example 18]
In the same manner as in Example 18, except that frozen burdock was used as a vegetable food material and 95 parts by weight of water was mixed instead of containing sorbitol in the sugar alcohol / enzyme mixed solution. After impregnating with the enzyme solution, the enzyme was inactivated by the same method, and the hardness was measured by the same method.
結果を表10に示す。 The results are shown in Table 10.
表10の結果から、注入で植物性食品素材中に酵素を導入する場合でも、酵素と糖アルコールを混合して用いると、効果的に植物性食品素材を摂取しやすく軟化できることがわかる。これは、植物性食品素材の内部での酵素の拡散・浸透を糖アルコールが促進しているためであると考えられ、酵素を直接、植物性食品素材の内部に導入する場合でも、糖アルコールの作用により、さらに効果的に軟化させることができることがわかる。 From the results of Table 10, it can be seen that even when an enzyme is introduced into a vegetable food material by injection, the enzyme and sugar alcohol can be mixed and used effectively and can be softened effectively. This is thought to be because sugar alcohol promotes the diffusion and permeation of the enzyme inside the vegetable food material. Even when the enzyme is introduced directly into the vegetable food material, the sugar alcohol It turns out that it can soften more effectively by an effect | action.
Claims (8)
A food using the plant food material according to claim 7.
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