JP6909045B2 - Beauty system and beauty method using it - Google Patents

Description

The present invention relates to a cosmetological system characterized by improving swelling of any part of a living body. The present invention also relates to a beauty method using a beauty system, which is characterized by improving swelling of an arbitrary part of a living body.

The lymphatic system of the skin is composed of a dense network of thin-walled vessels and plays a major role in the homeostasis of tissue fluids. For example, lymphatic vessels in the skin collect interstitial fluid, proteins, cells, etc. leaked from capillaries to maintain circulatory system homeostasis. Lymphatic dysfunction causes inadequate lymphatic transport and local fluid retention, resulting in lymphedema. Lymphatic dysfunction may also result in epidermal thickening, collagen and lipid deposition, progressive necrosis and increased susceptibility to infection.

So far, it has been shown that factors such as angiopoietin-1 and vascular endothelial growth factor C (VEGF-C) are involved in the stabilization of lymphatic function and lymphangiogenesis (for example, Non-Patent Document 1). And 2). In addition, it has been shown that giving physical stimulation (for example, electrical stimulation) to lymphatic endothelial cells causes changes in the morphology of lymphatic endothelial cells and phosphorylation of signal factors (for example, non-patent literature). 3).

Kaziya K.K. , Et al. , Promotion of lymphatic integrity by angiopoietin-1 / Tie2 signaling inflammation. Am. J. Pathol. 2012: 180: 1273-1282. Sawane M. and Kaziya K. Ultraviolet light-induced changes of lymphatic and blood vasculature in skin and their molecular mechanisms. Exp. Dermatol. 2012: 21 (Suppl1): 22-25. Kaziya K.K. , Et al. , Electric current-induced lymphatic activation. Exp. Dermatol. 2014 Dec; 23 (12): 936-8.

Conventionally, as a physical means for eliminating swelling in a living body, for example, a method of giving an electrical stimulus that contracts muscles to promote a pump function has been adopted. However, these stimuli are sometimes painful and difficult to apply to areas with thin skin tissue, such as the face. Therefore, an object of the present invention is to improve swelling of any part of a living body and to provide a beauty system and a beauty method that can be used safely.

As a result of studies by the present inventors, it has been found that swelling is improved when a voltage is applied in a living body in a current value and frequency in a predetermined range, and the present invention has been made.

That is, the present invention includes the following inventions.
[1] Power supply unit and
A voltage adjusting unit that controls the DC voltage supplied from the power supply so that the current value is within a predetermined range.
A pulse generator that converts the DC voltage into a pulsed DC current,
With at least a pair of conductors for applying the pulsed direct current,
A current measuring unit provided between the power supply unit and the conductor for measuring a current, and a current measuring unit.
Equipped with, here,
The at least one pair of conductors is brought into contact with any part of the living body,
The pulsed direct current, which is controlled by the voltage adjusting unit so that the current value in the predetermined range is in the range of 1 to 1000 μA and controlled by the pulse generating unit to be 1 to 1000 Hz, is 1 minute to 120 minutes. A beauty system characterized by improving swelling of any part of the living body by being applied.
[2] The beauty system according to [1], wherein the current value in the predetermined range is in the range of 10 to 500 μA.
[3] The beauty system according to [1] or [2], wherein the current value in the predetermined range is in the range of 100 to 250 μA.
[4] The beauty system according to any one of [1] to [3], wherein the pulsed direct current is a pulsed direct current of 10 to 500 Hz.
[5] The beauty system according to any one of [1] to [4], wherein the pulsed direct current is a pulsed direct current of 50 to 200 Hz.
[6] The beauty system according to any one of [1] to [5], further comprising a CPU for controlling the voltage adjusting unit and / or the pulse generating unit.
[7] The beauty system according to [6], wherein the CPU has a function of feedback controlling so that the power value output by the current measuring unit is within the predetermined range.
[8] The cosmetology system according to any one of [1] to [7], wherein improving the swelling of the living body acts on lymphatic endothelial cells and enhances lymphatic function.

[9] Using the beauty system according to any one of [1] to [8],
The at least one pair of conductors is brought into contact with any part of the living body,
Including the step of applying a pulsed direct current of 1 to 1000 Hz in the range of 1 to 1000 μA.
A cosmetological method characterized by improving swelling of any part of a living body.
[10] The beauty method according to [9], wherein the application step is carried out for 1 minute to 120 minutes.
[11] The beauty method according to [9], wherein the application step is carried out for 5 to 60 minutes.

According to the present invention, it is possible to eliminate swelling of any part of a living body by an electric current that does not cause contraction by muscles. In particular, since the current conductive to the living body is low enough not to contract the muscles, it is possible to safely improve swelling in any part of the living body (for example, face, lower limbs, upper arm, abdomen, etc.). It becomes.

FIG. 1 shows the beauty system of the present invention in one embodiment. FIG. 2 shows a block diagram of the beauty system of the present invention in one embodiment. FIG. 3 shows a block diagram of the beauty system of the present invention in one embodiment. FIG. 4 shows the morphological changes of lymphatic endothelial cells when an electrical stimulus is applied. (A) In one embodiment, it is a figure which shows the apparatus used when applying a voltage to a lymphatic endothelial cell (upper row: schematic view of the whole apparatus, lower row: cross-sectional view of a culture dish). Human lymphatic endothelial cells were cultured on fibronectin-coated ITO (indium tin oxide) plates and a voltage was applied. (B) Shows the morphology of lymphatic endothelial cells before application (control). (C) Shows the morphology of lymphatic endothelial cells immediately after application at a DC voltage of 2 V for 30 seconds. FIG. 5 is a diagram examining changes in intracellular signals after application with a DC voltage. (A) No stimulation (C: control) Phosphorylation of signal transduction substances (ERK, p38) in human lymphatic endothelial cells after stimulation at 1V, 2V, 3V for 30 seconds (P-ERK, P-p38). It is a figure which investigated about. (B) In human lymphatic endothelial cells without stimulation (C: control) or 15 minutes (15 m), 1 hour (1 h), 4 hours (4 h) or 24 hours (24 h) after stimulation for 2 V for 30 seconds. It is a figure which investigated the phosphorylation (P-ERK, P-p38) of a signal transduction substance (ERK, p38). FIG. 6 is a diagram showing the effect on human lymphatic cells when the amount of current and the frequency are changed. (A) It is a figure which shows the morphology of a lymphatic endothelial cell when a direct current voltage (2V, 1V) is applied using the apparatus shown in FIG. The current value was 200 μA when applied at a voltage of 2 V and 20 μA when applied at a voltage of 1 V. (B) Phosphorylation of p38 of lymphatic endothelial cells (p-p38) when a pulsed DC voltage (0.1 V, OFC 0.05 V) was applied at each frequency using the apparatus shown in FIG. It is a figure which shows. The values in parentheses indicate the current values when applied at each frequency. FIG. 7 is a diagram showing the migration of lymphatic endothelial cells before electrical stimulation (left) and after stimulation with a pulse current for 12 hours. FIG. 8 is a graph showing the state of proliferation of lymphatic endothelial cells by electrical stimulation. After electrical stimulation at 2V for 5 minutes or 2V for 10 minutes without stimulation (control), the cell cycle was determined using EdU Cell Proliferation Detection Kits (SIGMA-ALDRICH, Catalog # BCK-EDU 488). The number of cells that entered the proliferation phase was counted. The result is shown as cell proliferation rate (%). FIG. 9 is a model diagram showing changes in lymphatic vessels when the system and method of the present invention are used. The upper row shows the model before electrical stimulation, and the lower row shows the model after electrical stimulation. ○ in the upper figure indicates the part where the lymphatic endothelial cells overlap. By applying the present invention, a gap is created in the lymphatic vessels as shown in the lower figure, and waste products and water can be efficiently taken in. FIG. 10 shows the effect of improving the swelling of the lower limbs using the present invention. (A) It is a figure which shows the experiment using the system of this invention. (B) It is a graph which shows the swelling improvement effect of the lower limbs when the pulse DC current of 100Hz, 200μA is applied for 10 minutes using the system of this invention. FIG. 11 shows the effect of improving the swelling of the lower limbs using the present invention. The same experiment as in FIG. 10 was performed at each frequency (1 Hz, 100 Hz or 10000 Hz).

As used herein, "swelling" is used synonymously with edema, and is a phenomenon in which tissue fluid or lymph fluid is retained in the intracellular, intercellular spaces, or body cavities for some reason, and is observed in the subcutaneous tissue. .. Spontaneous swelling that occurs in daily life is thought to occur when the balance between fluid leakage from capillaries and fluid recovery by the veins and lymphatic system is impaired. The beauty system of the present invention and a beauty method using the same can act on the lymphatic endothelial cells constituting the lymphatic vessels of the lymphatic system to enhance the lymphatic vessel function, which occurs naturally in daily life. Effectively improves lymphatic swelling.

As used herein, the lymphatic system is a conduit network that carries a clear fluid called lymph, which is synonymous with terms used by those skilled in the art. In general, lymphatic vessels are a component of the complex systems that make up the lymphatic system. The lymphatic system includes lymph nodes, thoracic ducts, etc. in addition to lymphatic vessels. The lymphatic system is a trait that (1) removes excess fluid from tissues, (2) transports digested and absorbed lipids to the circulatory system, and (3) immunocompetent cells (eg, lymphocytes, monocytes, antibodies). It has functions such as producing cells). The lymphatic endothelial cells that make up the lymphatic vessels have a weaker bond than the vascular endothelial cells that make up the blood vessels, and have a structure in which tissue fluid and the like easily flow in from outside the duct, especially in the periphery. Peripheral lymph vessels have anchor filaments attached to them so that they are not over-crushed. The present invention has, in particular, the effect of acting on the lymphatic vessels, which plays the roles of (1) and (2).

Hereinafter, embodiments for carrying out the present invention will be described in more detail with reference to the drawings, but the technical scope of the present invention is not limited to the following embodiments.

<Beauty system>
The beauty system of the present invention will be described with reference to FIGS. 1 and 2. FIG. 1 is an external view of the beauty system 1 of the present invention in one embodiment. 2 and 3 show a block diagram of the beauty system 1 of the present invention in one embodiment.

First, the beauty system 1 of the present invention according to the embodiment will be described with reference to FIG. The beauty system 1 controls the current supplied from the power supply unit (not shown) so as to have a current value in a predetermined range, converts the current into a pulsed direct current, and converts the pulsed direct current into a living body S. It is provided with at least a pair of conductors 18 for applying the current and a current measuring unit 17 for measuring the current, and each of them is connected by a conductive cord 19 as shown in FIG. 1, for example. The electricity generated by the output unit 16 flows to the conductor 18 via the conductive cord 19. The pair of guides 18 are attached to any part of the living body, and the electricity emitted from one of the guides 18 passes through the living body and flows to the other guide 18. A current measuring unit 17 for measuring the amount of current flowing in the circuit is provided between the output unit 16 and the conductor 18. The current measuring unit 17 has a display unit 17a, and displays the current value flowing in the circuit. The guides 18 may be provided in pairs or more, and may be two pairs, three pairs, four pairs, five pairs, or more. As the conductor 18, a known conductor 18 may be used, and may be a needle-shaped conductor, a pad-shaped conductor, a clip-shaped conductor, or the like, which is intended to be conductive on the living body S. Preferably, it is a pad-shaped guide 18. The shape and material of the conductor 18 may be changed to a known one as appropriate according to the site of the living body S to be applied.

Although not shown in FIG. 1, the output unit 16 includes a voltage adjusting unit for controlling the current value within a predetermined range, and a pulse generating unit for converting a DC voltage into a pulsed DC current. The voltage adjusting unit and the pulse generating unit make it possible to pass a pulsed direct current through the living body S at a current value in a predetermined range. The output unit 16 includes an operation unit 11 for setting power on / off, voltage amount, current amount, frequency, waveform, amplitude, offset voltage, etc., and current or set voltage amount, current amount, frequency, waveform, amplitude. , A display unit 12 for displaying the offset voltage and the like is provided.

Next, the operation of the beauty system 1 according to the embodiment of the present invention will be described with reference to FIGS. 2 and 3. The beauty system 1 includes a CPU 113 that controls the operation of various electronic components and an output unit 116 that generates a pulsed direct current. Electricity is supplied to the CPU 113 from the power supply unit 110. On the display unit 112, items such as the current and / or set voltage amount, frequency (pulse), and duration are displayed. The CPU 113 operates based on the signal transmitted from the operation unit 111. The CPU 113 controls the output unit 116 (voltage adjusting unit 114 and pulse generating unit 115) to generate a pulsed direct current with a current amount in a predetermined range and a frequency in a predetermined range. The generated pulsed direct current flows through a conductive cord provided in the output unit 116 to one conductor 118, passes through the living body S, and flows to the other conductor 118. At this time, the amount of current flowing through the circuit is measured by the current measuring unit 117 provided in the circuit of the power supply unit 110 and the conductor 118. In this embodiment, it is possible to adjust the amount of current conductive to the living body S by operating the operation unit 111 according to the amount of current measured by the current measuring unit 117. In another embodiment, it may have a function of feedback control so that the power value output by the current measuring unit 117 becomes a current value in a predetermined range. In that case, the CPU 113 operates. , The output unit 116 is adjusted so that the amount of current is within a predetermined range (see FIG. 3).

In the present invention, the current conductive to the living body S is controlled so that the current value is in the range of 1 to 1000 μA. The current conductive to the living body S is preferably controlled to be in the range of 10 to 500 μA, and more preferably in the range of 50 to 200 μA. By controlling and applying the amount of electric current in the above range, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is extended, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved (see FIG. 9). In general, the resistance of the amount of electric current conducted to a living body changes depending on the composition of an arbitrary part of the living body (for example, the amount of water, the amount of muscle, the amount of fat, etc.), the contact area of a conductor, and the like. Therefore, it is preferable that the current value is adjusted in the range of 1 to 1000 μA in real time by the voltage adjusting unit 114 so that the effect of the present invention can be obtained most.

In the present invention, the pulsed direct current conducted on the living body S is controlled to be a pulsed direct current of 1 to 1000 Hz. The pulsed direct current conductive to the living body S is preferably controlled to be in the range of 10 to 500 Hz. By applying electricity with controlled pulsed direct current to the above range, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is extended, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved (see FIG. 9).

In the present invention, the pulsed direct current conductive to the living body S is preferably applied for 1 minute to 120 minutes, more preferably 5 minutes to 60 minutes. By applying a pulsed direct current to the living body S for the above time, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is extended, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved (see FIG. 9).

In one embodiment, the beauty system 1 of the present invention may be a device in which an output unit 16 and a current measurement unit 17 are separately provided as shown in FIG. 1, and is a device housed in one housing. It's okay.

The beauty system of the present invention is a system in which a pulsed direct current controlled so that the current value is in the range of 1 to 1000 μA and controlled to be 1 to 1000 Hz is applied for 1 minute to 120 minutes. This is a system in which an extremely weak pulsed direct current is applied as compared with the current value (for example, 10 mA or more) of a conventional device that causes muscle contraction. That is, it is completely different from the conventional swelling improving device that uses the pumping action of contracting muscles, and is a device that safely and effectively improves swelling of any part of the living body by a mechanism that acts directly on lymphatic endothelial cells. .. Since the beauty system of the present invention applies an extremely weak pulsed direct current, it causes almost no pain to the living body and can be applied to a portion where the skin tissue is thin, for example, the face.

<Cosmetology method using beauty system>
In one embodiment of the invention
Power supply and
A voltage adjusting unit that controls the DC voltage supplied from the power supply so that the current value is within a predetermined range.
A pulse generator that converts the DC voltage into a pulsed DC current,
With at least a pair of conductors for applying the pulsed direct current,
A current measuring unit provided between the power supply and the conductor for measuring current,
Using a beauty system equipped with
The at least one pair of conductors is brought into contact with any part of the living body,
Including the step of applying a pulsed direct current of 1 to 1000 Hz in the range of 1 to 1000 μA.
Provided is a cosmetological method characterized by improving swelling of any part of a living body.

In the present invention, the current conductive to the living body S is controlled so that the current value is in the range of 1 to 1000 μA, preferably in the range of 10 to 500 μA, and more preferably in the range of 50 to 200 μA. As a result, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is stretched, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved.

In the present invention, the pulsed direct current conductive to the living body S is controlled to be in the range of 1 to 1000 Hz, preferably 10 to 500 Hz. By controlling the pulse current within the above range, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is extended, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved (see FIG. 9).

In the present invention, the pulsed direct current conductive to the living body S is preferably carried out for 1 minute to 120 minutes, more preferably 5 minutes to 60 minutes. By applying a pulsed direct current to the living body S for the above time, it acts on lymphatic endothelial cells existing at any part of the living body, its morphology is extended, and lymphatic function is enhanced. As a result, gaps are formed in a part of the lymphatic vessels, and large waste products (for example, water, proteins, inflammatory cells, etc.) that cannot be collected conventionally are collected, and swelling is improved (see FIG. 9).

Hereinafter, the present invention will be described in more detail based on examples, but these are not intended to limit the present invention in any way.

<Example 1>
<Changes in lymphatic endothelial cells due to electrical stimulation>
A device capable of electrically stimulating lymphatic endothelial cells (LECs) using indium tin oxide (ITO) was prepared (Fig. 1 (A), Kajiya K., et al., Electrical current-induced dynamic activity). Exp. Dermatol. 2014 Dec; 23 (12): 936-8.). After electrical stimulation of LECs, cell morphology, intracellular Ca concentration, extracellular signal-regulated kinase (ERK), which is a signal transduction factor, and phosphorylation of p38 were examined.

When LECs (Promo Cell, Catalog No .: C-12216) were stimulated with a DC current of 1, 2 or 3 V for 30 seconds, the cells expanded most at 2 V (Fig. 4 (C)), and the intracellular Ca concentration increased. (Not shown), phosphorylation of ERK and p38 was observed (FIG. 5 (A)). Regarding the persistence of the response of LECs by electrical stimulation, phosphorylation of ERK and p38 was enhanced 15 minutes after electrical stimulation, and almost returned to the pre-stimulation state at 24 hours (Fig. 5 (B)).

Next, in the increase in Ca concentration in LECs by electrical stimulation, no change in concentration was observed when Ca was not present extracellularly (not shown). In addition, tetrodotoxin inhibited the increase in Ca concentration by electrical stimulation, but nifedipine did not, suggesting that voltage-gated Na channels, not voltage-gated Ca channels, are involved in the intracellular Ca influx. (Not shown). Furthermore, since the phosphorylation of ERK and p38 by electrical stimulation was suppressed by the inhibitor of focal adhesion kinase (FAK) involved in cell adhesion, it is considered that the electrical stimulation is transmitted to ERK and P38 via FAK. (Not shown).

Moreover, when the stimulation mode of electrical stimulation was changed, the phosphorylation of p38 was most enhanced at a pulse of 100 Hz and a current amount of 200 μA, suggesting that it is the optimum stimulation mode in LECs (FIG. 6). ).

Examination of the long-term effects of electrical stimulation on LECs revealed that electrical stimulation promoted the proliferation of LECs and had a polarity of migration to the positive pole side (Fig. 7).

In order to investigate how the application time of electrical stimulation affects cell proliferation, stimulation was performed for 5 minutes and 10 minutes with a pulse of 100 Hz and a current amount of 200 μA. As a result, it was clarified that the number of LECs cells increased in a time-dependent manner by stimulating for 5 minutes and 10 minutes.

From the above, it was suggested that LECs expand the morphology in response to electrical stimulation to promote the recovery function of lymphatic vessels, and that lymphatic vessels are regenerated to the positive pole side in the long term.
<Example 2>
<Verification of swelling improvement effect in humans by electrical stimulation>
The following experiment was carried out in order to confirm whether or not the swelling improving effect can be obtained in humans by applying a weak pulsed direct current in a living body.

Six healthy female volunteers aged 28-36 were enrolled in this study.

The volume of the lower limbs was determined using Archimedes' principle (the volume of the legs is equal to the amount of water replaced). Each subject was asked to slowly place his lower limbs in a clear acrylic container filled with lukewarm water at 32 ° C. to 34 ° C. to avoid a rapid vasoconstriction reaction. The height of the container is about 50 cm and can measure a volume of 1000-2500 mL accommodating the feet, ankles, and calves. Since the entire displaced volume overflows out of the container, accurate and reproducible measurements are possible (Fukuda K., Antiedema effects of Siberian ginseng in vitro and it's molecular measurement lymphatic system). . 2016 Jul; 36 (7): 689-695).

In this study, an electric generator (manufactured by NF Circuit Design Block Co., Ltd., model number: WF-1943A), a pair of electrode pads connected to it (manufactured by OMRON Corporation, model number: HV-JPAD), and a current measuring instrument (Kaise Co., Ltd.) A system constructed by a company, model number: KT-2011) was used (Fig. 10). As shown in FIG. 10, electrode pads were attached so as to sandwich both sides of the calf.

The pulsed direct current was adjusted to about 200 μA at 100 Hz with a DC current of 5 Vpp (amplitude) and OFS 5 to 9 V (offset voltage), and electrical stimulation was performed for 10 minutes. The lower limb volume was measured by the above procedure, and the degree of swelling was calculated. The state after sitting for 10 minutes was used as a control, and the comparison was made with the case where electrical stimulation was performed for 10 minutes in the sitting state.

As a result, it was clarified that the volume of the lower limbs was significantly reduced when the current was applied at 100 Hz and 200 μA for 10 minutes (FIG. 8 (B)).

<Example 3>
<Verification of swelling improvement effect in humans by electrical stimulation with different frequencies>
Further, using the same system as in Example 3, the current was adjusted to 200 μA, the frequency was changed to 1 Hz, 100 Hz or 10,000 Hz, and electrical stimulation was performed for 10 minutes. The lower limb volume in that case was measured according to the above procedure.

As a result, at any frequency (1 Hz, 100 Hz or 10,000 Hz), a significant swelling improvement effect was obtained as compared with the sitting position (control), but the effect was obtained with good reproducibility at 1 Hz and It was 100 Hz.

1 Beauty system 110 Power supply unit 11, 111 Operation unit 12, 112 Display unit 113 CPU
114 Voltage adjustment unit 115 Pulse generation unit 16, 116 Output unit 17, 117 Current measurement unit 17a Display unit 18, 118 Conductor 19 Conductive cord S Living body

Claims (4)

Power supply and
A voltage adjusting unit that controls the DC voltage supplied from the power supply unit so that the current value is within a predetermined range.
A pulse generator that converts the DC voltage into a pulsed DC current,
With at least a pair of conductors for applying the pulsed direct current,
A current measuring unit provided between the power supply unit and the conductor for measuring a current, and a current measuring unit.
Equipped with, here,
The at least one pair of conductors is brought into contact with any part of the living body,
The pulsed direct current controlled by the voltage adjusting unit so that the current value in the predetermined range is in the range of 100 to 250 μA and controlled by the pulse generating unit to be 50 to 200 Hz is 1 minute to 120 minutes. A beauty system characterized by improving swelling of any part of the living body by being applied. The beauty system according to claim 1, further comprising a CPU that controls the voltage adjusting unit and the pulse generating unit. The beauty system according to claim 2 , wherein the CPU has a function of feedback-controlling the power value output by the current measuring unit so that the current value falls within the predetermined range. The beauty system according to any one of claims 1 to 3 , wherein improving the swelling of the living body acts on lymphatic endothelial cells and enhances lymphatic function.

Images