JP6912638B2 - Slip agent for bees - Google Patents

Description

The present invention relates to a bee slip agent, a method for sliding a bee using the bee slip agent, and a bee capture container containing the bee slip agent.

Various types of pest catching containers have been known for a long time. For example, pest attracting and catching containers for small flying pests such as cockroaches and flies are widely used in general households, restaurants, factories, etc. Has been done.
Further, as a container for capturing bees, various shapes have been proposed. Specifically, Patent Document 1 describes a capture container in which an attraction capture composition for attracting bees is stored inside a cup-shaped container, and a lid having a bee entrance is attached to the upper part of the container. ing. Patent Documents 2 and 3 introduce a technique for improving the capture efficiency of bees by adjusting the visible light transmittance and color of the capture container. Patent Document 4 describes that the bee entrance is arranged at the center of the upper part of the capture container in order to prevent the once captured bee from escaping from the capture container. Further, Patent Document 5 describes that by using a bee-attracting component having a specific sugar concentration, a fermented odor due to putrefaction is generated and the capture efficiency is improved.
However, when liquid attractant traps are used in these bee capture containers, even if the bees fall into the liquid attractant traps, the bees will swim in the liquid attractant traps and liquid. There was a problem of crawling up from the surface and escaping from the container.

On the other hand, many bees have a habit of attracting other individuals of the same species by issuing an alarm pheromone when they or their colonies are in danger. Adhesive wasp traps that utilize this habit are designed to capture many bees by attaching live bees to the adhesive surface and generating an alarm pheromone. Extermination companies such as wasps may use this habit of bees to exterminate. However, when exterminating bees in ordinary people or households who are vulnerable to bees, there is a risk that the exterminating person will be attacked by the bees unless special attention is paid to this habit.
In the conventional bee catching container, there is also a problem that a large number of bees may invade around the catching container by issuing an alarm pheromone when the bee that escapes from the catching container as described above emits an alarm pheromone.

Japanese Unexamined Patent Publication No. 2004-073144 JP-A-2007-174964 JP-A-2009-247236 Japanese Unexamined Patent Publication No. 2014-103933 Japanese Unexamined Patent Publication No. 2007-176853

An object of the present invention is to prevent a bee from escaping from a bee trapping container.

As a result of intensive research to solve the above problems, the present inventor has found that a liquid composition adjusted to a specific contact angle reduces the decubitus function of a bee's leg, and this liquid composition. The above-mentioned problems have been solved by using a slip agent for bees made of.

Specifically, the gist of the present invention is as follows.
1. 1. A slip agent for bees, which comprises a liquid composition having a contact angle with respect to a glass surface of 40 degrees or less.
2. A method for sliding a bee, which comprises using a liquid composition having a contact angle with respect to a glass surface of 40 degrees or less.
3. 3. A bee trapping container, characterized in that a liquid composition having a contact angle with respect to a glass surface of 40 degrees or less is stored in a container having an opening.

The slip agent for bees of the present invention adheres to the leg tips of bees to reduce the function of the bee board. As a result, when the bee slip agent of the present invention is used in the bee catching container, the bees that have entered the inside of the bee catching container cannot crawl up from the liquid, or the inside of the bee catching container. Since it is not possible to climb from the highly smooth wall surface of the bee, it is effective in preventing the bees from escaping from the container. In addition, since bees cannot escape from the bee catching container, there is a concern that the bees that escaped like the conventional bee catching container will issue an alarm pheromone, causing a dangerous situation in which a large number of bees invade the surrounding area. do not have. That is, when the bee slip agent of the present invention is stored in a bee catching container as a bee catching liquid, or applied to the wall surface of a bee catching container containing a bee attracting liquid, a bee catching container is installed. It is useful in that it can capture only the bees around the place where it was struck and can be used safely by the user. The method of using the bee slip agent of the present invention is not limited to the method of storing it in the bee trapping container and the method of applying it. For example, by applying it to the wall surface, window surface, eaves, attic, etc. of a house, it can be expected that the bees will slide off from the place where the slip agent is applied and prevent the bees from foraging and nesting.

Hereinafter, the bee slip agent of the present invention, the method of sliding down a bee using this bee slip agent, and the bee capture container containing the bee slip agent will be described in detail.
The slip agent for bees of the present invention comprises a liquid composition in which the contact angle with respect to the glass surface is adjusted to 40 degrees or less.
The contact angle with respect to the glass surface in the present invention means a value obtained by measuring a sample by the following method with reference to the standard of "JIS R 3257" using a commercially available contact angle meter.
A predetermined amount of the liquid composition was dropped onto a slide glass that had been allowed to stand for 5 days in an environment of a temperature of 25 ° C. and a humidity of 60% under a temperature condition of 25 ° C., and the contact angle with respect to the glass surface after 20 seconds was measured. Measurement is performed using an instrument (for example, FACE contact angle meter CA-X type manufactured by Kyowa Interface Science Co., Ltd.).

The slip agent for bees of the present invention exhibits a function of lowering the decubitus function of the legs of a bee by comprising a liquid composition in which the contact angle with respect to the glass surface is adjusted to 40 degrees or less.
The tip of the bee's leg has a pair of sharp claws, and between the claws there is a suction cup that acts as a suction cup. In general, the claws have been developed to be applicable to walking on rough surfaces and the decubitus to be applicable to walking on smooth surfaces. Due to the presence of this bee board, bees can move around on a highly smooth wall surface such as a window glass by sticking to it, stopping or climbing.
The bee slip agent of the present invention adheres to the bee's leg tip to reduce the sucker-like function of the wasp, so that the bee slips on a highly smooth surface and slides down. It exerts the effect of making it.
Therefore, when the bee slip agent of the present invention is stored as a bee capture liquid in a container having an opening to form a bee capture container, or when applied to the wall surface of a bee capture container containing a bee attractant. The bees that have entered the container come into contact with the slip agent. Then, the slip agent for bees of the present invention does not function like a suction cup of the decubitus board, so that the slip agent cannot crawl out of the liquid or the wall surface with high smoothness inside the container for capturing bees. It is not possible to climb by slipping down from the bee catching container, and it is possible to prevent the escape from the bee catching container.

The bee slip agent of the present invention is not particularly limited in composition and composition ratio as long as it is a liquid composition in which the contact angle with respect to the glass surface is adjusted to 40 degrees or less, and any component can be blended. .. Although it is preferable to contain a surfactant in terms of adjusting the contact angle with respect to the glass surface, there is no limitation on the method for adjusting the contact angle with respect to the glass surface.
Any surfactant such as a nonionic surfactant, an anionic surfactant, a cationic surfactant, or an amphoteric surfactant can be used as long as the contact angle of the liquid composition with respect to the glass surface can be adjusted to 40 degrees or less. However, it can be used without particular limitation, but among them, a nonionic surfactant or an anionic surfactant is preferable.
Specifically, for example, as nonionic surfactants, sugar ester type, fatty acid ester type, vegetable oil type, alcohol type, alkylphenol type, polyoxyethylene-polyoxypropylene block polymer type, alkylamine type, bisphenol type. , Polyaromatic ring type. Examples of the anionic surfactant include carboxylic acid type, sulfonic acid type, sulfate ester type and phosphoric acid ester type. Examples of the cationic surfactant include ammonium type and benzalkonium type. Examples of amphoteric surfactants include betaine-type surfactants.
Suitable nonionic surfactants include polyglycerin fatty acid esters, polyoxyethylene alkyl ethers, polyoxyethylene polyoxypropylene glycols, polyoxyethylene polyoxypropylene alkyl ethers, polyoxyethylene aryl ethers, and polyoxyethylene sorbitan fatty acids. Examples thereof include esters, polyethylene glycol fatty acid esters, sucrose fatty acid esters, polyoxyalkylene-hardened castor oil, and fatty acid alkanolamides. Suitable anionic surfactants include polyoxyethylene alkyl ether carboxylates, alkyl sulfoacetates, alkyl sulfates, polyoxyethylene alkyl ether sulfates and the like. Suitable cationic surfactants include alkyldimethylbenzylammonium chloride. Suitable amphoteric surfactants include fatty acid amide propyl dimethyl amino acid betaine, alkyl amine oxides and the like.
In addition, as these surfactants, either alone or a mixture of 2 or more types can be used.

The bee slip agent of the present invention is a liquid composition in which the contact angle with respect to the glass surface is adjusted to 40 degrees or less, and is obtained by simply diluting the above-mentioned surfactant with water, a surfactant, and an insecticide. This includes those formulated together with the components generally added to the formulation, and those obtained by diluting the formulated product with water. Among them, as the slip agent for bees of the present invention, a formulation of the above-mentioned surfactant and other components is advantageous at the time of transportation and storage because it is compact and has excellent storage stability. Examples of the formulation type include liquid formulations such as oils, emulsions, wettable powders, and flowable agents (suspending agents in water, emulsions in water, etc.), and water used for dilution includes purified water, tap water, and the like. Ion-exchanged water, distilled water, filtered water, sterilized water, groundwater, well water and the like are used.
The amount of the surfactant to be blended in the slip agent for bees of the present invention is not particularly limited as long as it is a liquid composition in which the contact angle with respect to the glass surface is adjusted to 40 degrees or less. Generally, it is preferably contained in an amount of 0.005% by weight or more, more preferably 0.01% by weight or more, and particularly preferably 0.05% by weight or more based on the entire slip agent for bees. .. If the content of the surfactant is less than 0.005% by weight with respect to the entire slip agent for bees, it is difficult to adjust the contact angle with respect to the glass surface to 40 degrees or less, and the suction cup of the bee's decubitus plate. It is not possible to reduce the function such as.

The bee slip agent of the present invention can contain a known insecticide within a range in which the contact angle with respect to the glass surface can be adjusted to 40 degrees or less. By blending a known insecticide, the captured bees can be surely killed in the bee capture container in which the slip agent for bees of the present invention is stored as a liquid agent for capturing bees.
Known pesticides that can be compounded include, for example, natural pyrethrins, alesulins, resmethrins, flamethrins, prarethrins, terraresulins, phthalthrins, phenothrins, permethrins, ciphenothrins, cypermethrins, transfluthrins, metoflutrins, profluthrins, imiprothrins, empentrins , Pyrethroid compounds such as silafluofen, meperfluthrin, dimefurthrin; carbamate compounds such as propoxul and carbalyl; organic phosphorus compounds such as fenitrothione and DDVP; oxaziazole compounds such as methoxadiazone; phenylpyrazole compounds such as fipronil; Surfonamide compounds; neonicotinoid compounds such as imidacloprid and dinotefuran; insecticidal hormone-like compounds such as pyriproxyfen, metoprene and hydroprene; anti-young hormone-like compounds such as plecosene; novallon, diflubenzlone, etoxazole and the like Chitin synthesis inhibitors; various insecticides such as phytonchid, peppermint oil, orange oil, katsura oil, clove oil and other insecticidal essential oils, as well as synergistic agents such as cinepyrin and piperonyl butoxide. Can be used. Insect growth regulators such as insect juvenile hormone-like compounds, anti-juvenile hormone-like compounds, and chitin synthesis inhibitors, which are less repellent, can also be preferably used.

Among these known insecticides, when the insecticide is mixed with the slip agent for bees of the present invention, it may be diluted with water in order to adjust the contact angle with respect to the glass surface to 40 degrees or less. Considering that there are some cases, those having high water solubility may be preferable in the formulation. Examples of insecticides having such water solubility include acephate, bamidthione, methidathion (DMTP), phenobucarb (BPMC), ethiophencarb, cultap, thiocyclam, imidacloprid, thiacloprid, silomadine, hostizate, acetamiprid, thiamethoxam, carbaryl. , Crossianidin, Pimetrodin, Dinotefuran and the like. Among these, for example, dinotefuran (water solubility at 20 ° C.: about 54,000 ppm), thiamethoxam (water solubility at 20 ° C.: about 4100 ppm), imidacloprid (water solubility at 20 ° C.: about 510 ppm), phenobucarb (BPMC, water at 20 ° C.). A water solubility of 500 ppm or more at 20 ° C. such as solubility: about 610 ppm) is more suitable as an insecticide to be blended in the bee slip agent of the present invention.

The bee slip agent of the present invention can contain a known attracting component within a range in which the contact angle with respect to the glass surface can be adjusted to 40 degrees or less. Known attractant ingredients that can be blended include, for example, balsamic vinegar, apple vinegar, rice vinegar, brown rice vinegar, lees vinegar, soybean vinegar, black vinegar, wine vinegar, sudachi vinegar, red vinegar, persimmon vinegar, malt vinegar, and purple potato vinegar. , Vinegar such as sugar cane vinegar, lactic acid products (lactic acid bacteria beverages, etc.), sugars, starch sugars, honey, fruits, processed fruit products, fruit juice, fruit juice beverages, fruit liquor, alcoholic beverages, sake lees, seafood, processed seafood products, It may be based on seafood extract, meat, processed meat product, meat extract, vinegar and the like. Among the above-mentioned attracting components, liquid ones are particularly preferable. Further, if a moisturizing component such as an aliphatic polyhydric alcohol such as glycerin, a sugar alcohol such as sorbitol, and a thickening polysaccharide such as xanthan gum is contained, the effect of the attracting component can be exhibited for a long period of time.

The slip agent for bees of the present invention can contain components generally added to the preparation within a range in which the contact angle with respect to the glass surface can be adjusted to 40 degrees or less. Examples of components generally added to a pharmaceutical product include stabilizers, preservatives, colorants, accidental swallowing agents, liquid carriers and the like. Examples of the stabilizer include antioxidants such as dibutylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), ascorbic acid and the like. Examples of preservatives include sorbic acid, sorbate, parahydroxybenzoic acid esters, thiabendazole and the like. Examples of the colorant include caramel color, crocin color, anthocyanin color, safflower color, flavonoid color, red No. 2, red No. 3, yellow No. 4, yellow No. 5, and the like. Examples of the accidental ingestion preventive agent include denatonium benzoate and the like.

Examples of the liquid carrier used in the formulation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, benzyl alcohol, ethylene glycol, etc.) and ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, diethylene glycol). Monoethyl ether, propylene glycol monomethyl ether, tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, ethyl lactate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatics Or aliphatic hydrocarbons (xylene, toluene, alkylnaphthalene, phenylxysilyl ethane, kerosine, light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (acetriform, acetonitrile, etc. Isobutyronitrile, etc.), sulfoxides (dimethylsulfoxide, etc.), heterocyclic solvents (sulfolane, γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, etc. 1,3-Dimethyl-2-imidazolidinone), acid amides (N, N-dimethylformamide, N, N-dimethylacetamide, etc.), alcoholidene carbonates (propylene carbonate, etc.), vegetable oils (soybean oil, cottonseed oil, etc.) , Vegetable essential oils (orange oil, hisop oil, lemon oil, etc.), and water.

The bees in which the bee slip agent of the present invention exerts an excellent slip effect are mainly targeted at bees belonging to the family Vespidae, but membranes of hornets, bear bees, bee bees, ammophilinae, and wasps. Those belonging to the Hymenoptera pest can be mentioned. Among them, as the wasp family bee, a bee belonging to the wasp subfamily (Vespinae) and the paper wasp subfamily (Polistinae) can be mentioned.
Examples of bees belonging to the hornet subfamily include giant hornets, hornets, hornets, hornets, vespa ducalis, hornets, hornets, fern hornets, and wasps.
In addition, as bees belonging to the paper wasp subfamily, for example, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps, paper wasps. Indigenous species can be mentioned. In addition to these indigenous species, the Asian hornet that invaded Tsushima and Kitakyushu can also be mentioned. Although the aggression and toxicity of paper wasps are smaller than those of wasps, they are similar to wasps in that they are subject to control because they may nest in private houses and there are cases of death due to stinging.

<About the bee catching container>
The bee slip agent of the present invention can be stored in a container having an opening as a bee catching liquid and used as a bee catching container, or can be applied to the wall surface of a bee catching container containing a bee attracting liquid. can.
The shape and size of the container to be stored or applied are not limited as long as the container can contain the slip agent for bees and the bee attractant of the present invention and has an opening through which the bees can enter the inside of the container. The form may be suitable for the place of use and the method of use. Examples of the material of this container include glass, metal, plastic and the like. In particular, the wall surface inside the container is preferably a smooth surface without protrusions so that the captured bees cannot climb using the claws of the legs and escape from the container. Further, in order to reduce the sucker-like function of the wasp's decubitus by the bee slip agent of the present invention, the wall surface inside the container should be a smooth surface like glass, which maximizes the effect of the present invention. It is more preferable because it is limited.
As an example of the mode of the container having an opening, there is a mode in which a lid covering the opening of the container is provided, and either the lid or the container has an opening through which a bee can enter. The opening may have a size and shape that allows bees to easily enter the container, and the dimensions (diameter, width, length) of the opening are preferably set to 20 mm or more and 35 mm or less, and 25 mm or more and 30 mm. It is more preferable to set as follows. If the opening is smaller than 20 mm, it is difficult for large bees such as the giant hornet to invade through the opening, and the capture efficiency is lowered. If the opening is larger than 35 mm, butterflies, moths, beetles, etc. other than bees will invade the container. It is preferable to form a plurality of openings in the container so that the bees can easily enter the container, but the number of openings may be 2 or more and 5 or less depending on the size of the container. preferable. Further, in order to prevent the bees that have entered the container from easily escaping to the outside of the container, the opening is preferably funnel-shaped or the like.
The container may be a transparent or translucent window-equivalent container so that the number of bees captured can be visually observed, or a transparent or translucent container, which makes the captured bees difficult to see and suppresses discomfort. can.
When the bee catching container is installed outdoors, rainwater or the like infiltrates and dilutes the bee slip agent of the present invention, so that the effect is not reduced, so that rainwater has a space for the opening. It is preferable to provide a covering portion for preventing the intrusion of such as.
It is preferable that the bee trapping container of the present invention is not exposed to direct sunlight, is hung from the ground at a distance of 1 to 3 m, or is fixed to a fence or the like.

Hereinafter, the present invention will be described in more detail with reference to formulation examples, test examples, and the like, but the present invention is not limited to these examples.
In the examples, unless otherwise specified, parts mean parts by weight.

<Measurement 1 of contact angle with respect to glass surface and slip test>
(1) Preparation of test sample Each component shown in Table 1 below was used as a test sample.
(2) Measurement of contact angle with respect to glass surface FACE contact angle meter CA on a slide glass (26 mm x 76 mm, manufactured by Matsunami Glass Ind. Co., Ltd., S1226) left to stand in an environment of temperature 25 ° C. and humidity 60% for 5 days. Using the attached syringe of -X type (manufactured by Kyowa Interface Science Co., Ltd.), about 1.8 μL of each test sample was dropped, and the contact angle 20 seconds after the dropping was measured by the FACE contact angle meter CA-X type (Kyowa Interface). Measured by Kagaku Co., Ltd.). All measurements were carried out at room temperature of 25 ° C., 3 times each. Table 1 also shows the details of the components of the test sample and the average value of the measured values of the contact angle with respect to the glass surface.
(3) Slip test 100 μL of the test sample was dropped into a 15 mL test tube (manufactured by AGC Technoglass Co., Ltd.) and shaken to apply it to the entire wall surface. After putting the Western honey bee (hereinafter referred to as "test bee") that has been acclimatized for 30 minutes after capture, stand vertically, observe the state of the test bee for 1 minute, and determine whether the test tube can be climbed according to the following evaluation criteria. Evaluated according to.
[Evaluation criteria]
〇: I couldn't climb the wall by sliding, and I couldn't reach the top after 1 minute.
X: The wall surface can be climbed and the top surface can be reached within 1 minute.

表１中のシリコーン系化合物、エステル系溶剤、界面活性剤１〜６の詳細は、以下のとおりである。
ジメチルポリシロキサン１：旭化成ワッカーシリコーン株式会社製、「ＤＭ１ ｐｌｕｓ」
ポリエーテル変性シリコーン：ケーアイケミカル株式会社製、「ＳＩＬＳＯＦＴ ８７０」
ジメチルポリシロキサン２：旭化成ワッカーシリコーン株式会社製、「ＡＫ１０００」
乳酸エチル：株式会社武蔵野化学研究所製
ミリスチン酸イソプロピル：東京化成工業株式会社製
界面活性剤１：ラウリン酸ポリグリセリル（１０）、ＨＬＢ:１５．５
界面活性剤２：ポリオキシエチレンアルキル（Ｃ１２〜１４）エーテル、ＨＬＢ:１３．５
界面活性剤３：ラウリン酸ポリグリセリル（５）、ＨＬＢ:１５．８
界面活性剤４：ラウリン酸ポリグリセリル（１０）、ＨＬＢ:１７．１
界面活性剤５：ポリオキシエチレン硬化ヒマシ油、ＨＬＢ:１４．０
界面活性剤６：ポリオキシエチレンフィトスチロール、ＨＬＢ:１８．０

Details of the silicone compounds, ester solvents, and surfactants 1 to 6 in Table 1 are as follows.
Dimethylpolysiloxane 1: Asahi Kasei Wacker Silicone Co., Ltd., "DM1 plus"
Polyether-modified silicone: "SILSOFT 870" manufactured by KI Chemical Co., Ltd.
Dimethylpolysiloxane 2: Asahi Kasei Wacker Silicone Co., Ltd., "AK1000"
Ethyl lactate: manufactured by Musashino Chemical Laboratory Co., Ltd. Isopropyl myristate: manufactured by Tokyo Chemical Industry Co., Ltd. Surfactant 1: Polyglyceryl laurate (10), HLB: 15.5
Surfactant 2: Polyoxyethylene alkyl (C12-14) ether, HLB: 13.5
Surfactant 3: Polyglyceryl laurate (5), HLB: 15.8
Surfactant 4: Polyglyceryl laurate (10), HLB: 17.1
Surfactant 5: Polyoxyethylene hydrogenated castor oil, HLB: 14.0
Surfactant 6: Polyoxyethylene phytostyrene, HLB: 18.0

As shown in Table 1, it was confirmed that the test bee could not climb the wall surface when the test sample having a contact angle with the glass surface of 40 degrees or less was present on the wall surface regardless of the type of the component of the test sample. When the test sample is only water, the test bee roams freely on the wall surface, whereas in the test sample where the contact angle with the glass surface is 40 degrees or less, the test bee cannot stay on the wall surface and flaps its wings. It was possible to visually confirm how the movement of trying to climb was repeated. From this, it was clarified that the test sample having a contact angle with respect to the glass surface of 40 degrees or less has an action of reducing or not functioning the decubitus function of the bee's leg.

<Measurement 2 of contact angle with respect to glass surface and confirmation test of escape prevention effect>
(1) Preparation of test sample Each of the surfactants 1 to 6 used in the above-mentioned "Measurement 1 of contact angle with glass surface and slip test" was mixed with water to obtain a uniform aqueous solution of 0.06% by weight. This was designated as test samples 1 to 5 and 7.
Test sample 6 was prepared by adding sake, edible vinegar, and sugar to a bee attractant in which a weight ratio of 2: 1: 1 was added so that the concentration of the surfactant 1 was 1.0% by weight. This test sample 6 is the same as the "mixture" in Table 1 of the above-mentioned "Measurement 1 of contact angle with respect to glass surface and slip test".
Only purified water was used as the test sample 8, and a commercially available bee attractant diluted 5 times with water according to the usage method described in the commercially available product was used as the test sample 9.
(2) Measurement of contact angle with respect to the glass surface The contact angle with respect to the glass surface was measured in the same manner as in the above-mentioned "Measurement of contact angle with respect to glass surface 1 and slip test". All measurements were carried out at room temperature of 25 ° C., 3 times each, and the average value is shown in Table 2.
(3) Confirmation test of the effect of preventing escape from the bee capture container 250 mL of each test sample was placed in an empty container (made of polypropylene) of a commercially available capture container containing a bee attractant. One Western honey bee (hereinafter referred to as "test bee") was put into the test sample liquid surface, and once the test bee was drowned under the liquid surface, it was observed whether or not it escaped from the liquid surface. The test was performed 5 times, and the value obtained by dividing the number of times that escape was not possible by the number of tests 5 is shown in Table 2 as the escape prevention rate (%).

As shown in Table 2, it was clarified that the test samples 1 to 6 having a contact angle with the glass surface of 40 degrees or less have an extremely high effect of preventing escape from the bee trapping container. When combined with the results of "Measurement 1 of contact angle with glass surface and slip test" above, test specimens with a contact angle with glass surface of 40 degrees or less are captured by reducing or stopping the decubitus function of the bee's leg. It was confirmed to prevent escape from the container.

<Measurement of contact angle with glass surface 3, slip test and confirmation test of escape prevention effect>
Confirmation tests were conducted on various types of surfactants in order to confirm the relationship between the contact angle with respect to the glass surface and the slip effect and the escape prevention effect.
(1) Preparation of test sample Each of the surfactants 7 to 15 shown in Table 3 below was mixed with water to obtain a 1% by weight uniform aqueous solution, which was used as test sample 10 to 18.
(2) Measurement of contact angle with respect to the glass surface The contact angle with respect to the glass surface was measured in the same manner as in the above-mentioned "Measurement of contact angle with respect to glass surface 1 and slip test". All measurements were carried out at room temperature of 25 ° C., 3 times each, and the average value is shown in Table 3.
(3) A slip test was performed in the same manner as in the above-mentioned "Measurement 1 of contact angle with respect to glass surface and slip test", and the results were evaluated in the same manner. The evaluation results are shown in Table 3.
(4) Confirmation test of escape prevention effect from bee capture container Similar to the above "Measurement of contact angle to glass surface 2 and confirmation test of escape prevention effect", confirmation test of escape prevention effect from bee capture container went. The test was performed 5 times, and the value obtained by dividing the number of times that escape was not possible by the number of tests 5 is shown in Table 3 as the escape prevention rate (%).

表３中の界面活性剤７〜１５の詳細は、以下のとおりである。
＜非イオン性界面活性剤＞
界面活性剤７：ヤシ油脂肪酸ジエタノールアミド
界面活性剤８：モノラウリン酸ポリオキシエチレン（２０）ソルビタン
界面活性剤９：ポリオキシエチレン（３６０）ポリオキシプロピレン（７０）ブロックポリマー
＜陰イオン性界面活性剤＞
界面活性剤１０：ポリオキシエチレン（２）ラウリルエーテル硫酸ナトリウム
界面活性剤１１：ポリオキシエチレン（４．５）ラウリルエーテル酢酸ナトリウム
界面活性剤１２：ラウリル硫酸ナトリウム
＜陽イオン性界面活性剤＞
界面活性剤１３：アルキル（Ｃ１２〜１４：８０％）ジメチルベンジルアンモニウムクロライド
＜両性界面活性剤＞
界面活性剤１４：ヤシ油脂肪酸アミドプロピルベタイン
界面活性剤１５：ラウリルジメチルアミンオキシド

Details of the surfactants 7 to 15 in Table 3 are as follows.
<Nonionic surfactant>
Surfactant 7: Palm oil fatty acid diethanolamide Surfactant 8: Polyoxyethylene monolaurate (20) sorbitan Surfactant 9: Polyoxyethylene (360) Polyoxypropylene (70) Block polymer <Anionic surfactant ＞
Surfactant 10: Polyoxyethylene (2) Sodium Lauryl Ether Sulfate Surfactant 11: Polyoxyethylene (4.5) Sodium Lauryl Ether Acetate Surfactant 12: Sodium Lauryl Sulfate <Cationic Surfactant>
Surfactant 13: Alkyl (C12-14: 80%) Dimethylbenzylammonium chloride <Amphoteric surfactant>
Surfactant 14: Coconut oil fatty acid amide propyl betaine Surfactant 15: Lauryl dimethylamine oxide

As shown in Table 3, nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants are classified according to whether or not they dissociate into ions when dissolved in water. Regardless of the type, if there is a test sample on the wall surface that has a contact angle of 40 degrees or less with respect to the glass surface, it is clear that the test bee cannot climb the wall surface and the effect of preventing escape from the bee capture container is extremely high. It became.

<Slip test for paper wasps>
(1) Test Specimens Test Specimens 6 and 8 of the above-mentioned "Measurement 2 of contact angle with respect to glass surface and confirmation test of escape prevention effect" were used.
(2) Test method Except that the test bee was changed to paper wasp and a 50 mL test tube (manufactured by AGC Techno Glass Co., Ltd.) was used instead of the 15 mL test tube (manufactured by AGC Techno Glass Co., Ltd.), the above "for the glass surface" The test was carried out in the same manner as in "Measurement 1 of contact angle and slip test".
(3) Test Results When the test sample 6 (contact angle with respect to the glass surface: 32.9 degrees), which is a specific example of the slip agent for bees of the present invention, was used, the paper wasps could not slide up the wall surface. It was confirmed that the top surface could not be reached after 1 minute. On the other hand, when a test sample 8 (contact angle with respect to the glass surface: 52.3 degrees) different from the slip agent for bees of the present invention is used, the paper wasp can climb the wall surface and reach the top surface. Was confirmed.
From this test result, it was clarified that the slip agent for bees of the present invention has an action of reducing or stopping the decubitus function of the leg of a bee regardless of the type of wasp.

<Outdoor confirmation test>
A confirmation test was conducted outdoors in order to confirm the bee capture effect in an actual usage mode using a bee capture container containing the bee slip agent of the present invention as a bee capture liquid.
(1) Test Specimen The surface activity of the test Specimen 6 (contact angle with the glass surface: 32.9 degrees, Example) and the test Specimen 6 of the above-mentioned "Measurement 2 of contact angle with glass surface and confirmation test of escape prevention effect" A test sample 19 containing no agent 1 (sake: edible vinegar: sugar = 2: 1: 1 (weight ratio), contact angle with respect to the glass surface: 51.4 degrees, comparative example) was used, and this was commercially available. An empty container (made of polypropylene) of a capture container containing a bee attractant containing 250 mL of each of test samples 6 and 19 was used.
(2) Test method Two test plots (test plots 1 and 2) were set in the wooded area, 3 m apart from the above example and the comparative example, and placed at a height of 2 m above the ground, and captured in a container. The state of the bees was confirmed, and the presence or absence of surviving individuals was observed one week later.

(3) Observation Results In the example using the test sample 6 (contact angle with respect to the glass surface: 32.9 degrees), which is a specific example of the wasp slip agent of the present invention, both test groups 1 and 2 are for bee capture. All bees such as wasps captured in the container could not crawl up from the test sample 6 and did not survive. On the other hand, in the comparative example in which the test sample 19 (contact angle with respect to the glass surface: 51.4 degrees) different from the wasp slip agent of the present invention was used, both test groups 1 and 2 were inside the wasp capture container. Some bees, such as wasps, were alive. Further, in the test plots 1 and 2 of the comparative example, it was visually confirmed that the wasps such as wasps captured in the bee trapping container escaped to the outside of the container, and that many bees came flying after that. .. It is presumed that the arrival of many wasps such as wasps is due to the fact that the bees such as wasps that escaped from the container for capturing wasps issued an alarm pheromone and therefore called in bees such as wasps.

Claims (3)

A slip agent for bees, which comprises a liquid composition having a contact angle with respect to a glass surface of 40 degrees or less (however, a composition containing a surfactant is excluded). A method for sliding a bee, which comprises using a liquid composition having a contact angle with respect to a glass surface of 40 degrees or less (excluding a composition containing a surfactant). A liquid composition having a contact angle with respect to a glass surface of 40 degrees or less (excluding a composition containing a surfactant) is stored in a container having an opening for capturing bees. container.