JP7640241B2 - Yeast culture medium, skin preparation for external use containing said culture medium, skin cosmetic preparation for external use containing said culture medium, and method for producing said culture medium - Google Patents

Description

The present invention relates to a yeast culture medium that can be used as an external preparation for the skin or a skin cosmetic, an external preparation for the skin containing the culture medium, a skin cosmetic containing the culture medium, and a method for producing the culture medium.

Aging care and prevention are of great interest to both men and women, and a variety of cosmetics, food products, and other products designed specifically for this purpose are on the market. As the amount of sebum and moisture in the skin decreases with age, the stratum corneum on the skin's surface loses its moisturizing power, and it is said that fine wrinkles and dry skin are more likely to appear due to dryness. In addition, the effects of air conditioning and heating expose the skin to dryness all year round, so there is growing interest in topical skin preparations and skin cosmetics that have high moisturizing and anti-aging effects.

Traditionally, skin cosmetics have often attempted to enhance the skin's moisturizing effect and make the skin lustrous by blending moisturizers and oil components such as polyhydric alcohols such as glycerin, hyaluronic acid, ceramide, polysaccharides, yeast extract, yeast culture liquid, petrolatum, and jojoba oil. Topical skin preparations also contain oily components such as white petrolatum, liquid paraffin, isopropyl myristate, beeswax, lanolin, and stearic acid as bases other than the main drug. The bases in topical skin preparations not only enhance the medicinal effects of the main drug, but also contribute to the protective and moisturizing effects of the skin.

Specifically, among skin cosmetics, many anti-aging cosmetics have been proposed from various angles to improve wrinkles, dullness, sagging, and other skin conditions that deteriorate with age (see Patent Documents 1 to 6).

By the way, Betula platyphylla (white birch) is said to be native to the northern temperate zone of East Asia, and grows mainly in cool, naturally rich areas such as Hokkaido. As described below, bark extracts and sap filtrates have been proposed as ingredients for cosmetics. For example, Patent Documents 7 and 8 disclose a whitening composition that uses birch bark and birch extract extracted with a hydrophilic solvent such as ethanol to improve arteriosclerosis and cataracts caused by aging and to have a whitening effect. Patent Document 9 discloses blended cosmetics that contain gel microparticles containing birch sap components, and Patent Document 10 discloses bath additives and footbath additives that contain birch sap as an ingredient.

On the other hand, there are many prior arts in which yeast culture fluid or extracts extracted from cultured yeast are used in skin external preparations and skin cosmetics, and many blended products are sold to general consumers. For example, Patent Document 11 discloses a yeast extract for cosmetics, which is obtained by culturing yeast in a medium containing a large amount of glutamic acid, cysteine, and glycine, and extracting the yeast extract with a polyhydric alcohol without digesting the yeast with enzymes or proteases in the yeast cells. Patent Document 12 discloses a composition having a heat shock protein 70 production induction effect by using a yeast cell extract that has accumulated a high concentration of zinc compounds in the cells. Patent Document 13 discloses a culture obtained by culturing yeast in a medium containing hyaluronic acids selected from the group consisting of hyaluronic acid, hyaluronic acid derivatives, and salts thereof, and a cosmetic and skin external product containing the culture. Patent Document 14 discloses a fermentation liquid using nicotinamide mononucleotide and yeast, which has excellent moisturizing and texture improving effects and is also less irritating.

The culture medium for culturing yeast contains a carbon source such as glucose or other sugar as a substrate, a nitrogen source such as yeast extract, peptone, or corn steep liquor, and minerals such as potassium and calcium. Patent documents 15 and 16 disclose the use of a concentrate or dried product of a culture medium in which yeast is cultured in a medium containing glucose, skim milk, and yeast extract as substrate components, as a pharmaceutical or cosmetic for skin. Patent document 17 discloses the use of a soymilk fermentation product obtained by fermenting soymilk with yeast as a substrate component as a blastocyte proliferation agent, epidermal keratinocyte proliferation agent, collagen production promoter, hyaluronic acid production promoter, and cosmetic.

JP 2019-203033 A JP 2007-291102 A JP 2008-169196 A JP 2008-255020 A JP 2008-260721 A JP 2009-040690 A Japanese Patent Application Publication No. 10-15244 JP 2001-335498 A JP 2003-321323 A JP 2006-131546 A JP 2006-347988 A JP 2014-108945 A JP 2015-221756 A JP 2018-145167 A Special Publication No. 61-43033 Special Publication No. 3-48166 JP 2017-155029 A

However, birch sap does not have sufficient moisturizing or texture-improving effects when used as a skin topical agent or skin cosmetic. Furthermore, there is no known technology for culturing yeast in a medium containing birch sap as a substrate, and it is completely unclear what effects a culture solution in which yeast has been cultured would have. In light of this situation, the present invention aims to clarify the excellent effects of a yeast culture solution using birch sap, and to provide a yeast culture solution having such excellent effects, a skin topical agent containing said culture solution, a skin cosmetic containing said culture solution, and a method for producing said culture solution.

As a result of intensive research, the inventors of the present invention discovered that when they cultured yeast in a medium containing birch sap, the resulting culture solution had the effect of maintaining and improving the condition of the skin, such as improving the skin's texture and moisturizing the skin, and thus completed the present invention. The present invention encompasses the following:

(1) A culture medium in which yeast is grown in a medium containing birch sap.
(2) The culture medium according to (1), wherein the birch sap is sap obtained from Betula platyphylla.
(3) A culture medium according to (1) or (2), characterized in that the yeast is one or more yeasts selected from the group consisting of yeasts belonging to the genus Galactomyces, yeasts belonging to the genus Saccharomycopsis, and yeasts belonging to the genus Saccharomyces.
(4) A skin topical preparation containing the culture medium described in any one of (1) to (3) above.
(5) A skin cosmetic comprising the culture medium according to any one of (1) to (3) above.
(6) The skin cosmetic composition according to (5), which exhibits at least one effect selected from the group consisting of a moisturizing effect, a texture improving effect, and a dullness improving effect.
(7) A method for producing a culture medium, characterized in that during the process of culturing yeast in a medium containing birch sap, low molecular weight sugars in the birch sap are consumed as a nutrient source for the yeast, resulting in a lower concentration of low molecular weight sugars in the birch sap compared to before the start of cultivation.

The culture solution according to the present invention can maintain and improve the condition of the skin, such as improving the skin's texture and moisturizing the skin. In particular, the culture solution according to the present invention is extremely effective in maintaining and improving the skin's condition, compared to birch sap in which yeast fungi are not cultured.

The skin topical preparation and skin cosmetic according to the present invention contain the culture solution, and therefore can maintain and improve skin conditions, such as improving skin texture and moisturizing effects. Furthermore, the method for producing a culture solution according to the present invention can produce a culture solution that can maintain and improve skin conditions, such as improving skin texture and moisturizing effects, and that has a low concentration of low molecular weight sugars.

FIG. 2 is a characteristic diagram showing the results of measuring the glucose concentration for Production Examples 1 to 4 prepared in Example 1.

The present invention is described in detail below.

The culture medium to which the present invention is applied is a culture medium in which yeast fungi are cultured in a medium containing birch sap. Here, birch sap means sap collected from birch. Examples of birch include broadly defined white birch (scientific name: Betula platyphylla, synonym: Betula pendula var. platyphylla), American white birch (scientific name: Betula papyrifera), European white birch (scientific name: Betula pendula, also known as weeping birch), European white birch (scientific name: Betula pubescens, also known as European birch), and Japanese white birch (scientific name: Betula szechuanica, synonym: Betula pendula var. szechuanica). Among these, the white birch sap is preferably sap collected from white birch classified as broadly defined white birch (scientific name: Betula platyphylla). Examples of Betula platyphylla classified as Betula platyphylla in the broad sense include Betula platyphylla var. platyphylla, Betula platyphylla var. japonica, Betula platyphylla var. japonica f. laciniata, Betula platyphylla var. kamtschatica, and Betula platyphylla var. mandshurica.

The birch sap may be collected from one of the above-mentioned species of birch, or may be a mixture of sap collected from multiple species of birch. Birch sap is collected, for example, by drilling holes or making linear incisions in the trunks of birch trees. The composition of the collected sap may vary depending on the habitat of the tree and the season. For example, the collected sap is about 99% water, with the remaining solids being monosaccharides such as glucose and fructose, and minerals such as potassium, sodium, calcium, magnesium, manganese, iron, zinc, phosphorus, barium, silicon, boron, lanthanum, strontium, thorium, aluminum, and chromium. In addition, birch sap may contain other amino acid components such as glutamic acid, aspartic acid, valine, isoleucine, methionine, leucine, lysine, phenylalanine, glycine, alanine, threonine, arginine, cystine/cysteine, proline, serine, tyrosine, and histidine, as well as organic acids such as malic acid and succinic acid, proteins, and polysaccharides.

The yeast fungus to be cultured in the medium containing birch sap is not particularly limited, and any of the yeast fungus disclosed in The Yeasts, Fifth Edition: A Taxonomic Study and the like can be used. Examples of yeast fungus include yeasts of the genera Candida, Galactomyces, Saccharomycopsis, Saccharomyces, and Pichia. In particular, one type of yeast fungus may be cultured in the medium containing birch sap, but multiple yeast fungus may also be used for culture simultaneously or at different times.

In particular, it is preferable to use, as the Galactomyces yeast among the yeasts, for example, Galactomyces reessii, Galactomyces geotrichum, Galactomyces candidus, Galactomyces pseudocandidus, etc. Furthermore, it is preferable to use, as the Saccharomycopsis yeast among the yeasts, for example, Saccharomycopsis capsularis, Saccharomycopsis crataegensis, Saccharomycopsis malanga, etc. Furthermore, as the yeast fungus of the genus Saccharomyces, it is preferable to use, for example, Saccharomyces cerevisiae, Saccharomyces bacillaris, etc. As the yeast fungus of the genus Saccharomyces, commonly used beer yeast, sake yeast, wine yeast, baker's yeast, etc. may also be used. As for these yeast fungus examples, one type may be used alone, or two or more different types of yeast fungus may be used in combination.

The birch sap contained in the yeast culture medium may be in any form, for example, the squeezed raw liquid may be used, or the raw liquid may be crudely filtered. The birch sap used is preferably one from which impurities, non-soluble solids have been removed by filtration. Furthermore, the birch sap may be one obtained by diluting the raw liquid with a solvent such as water to a specified ratio, or one obtained by concentrating the same to a specified ratio. However, it is most preferable to use the birch sap at the component concentrations at which it was collected without diluting or concentrating it.

It is also preferable that the birch sap is sterilized before the yeast is cultured. That is, when the birch sap is used by adding it to a normal yeast culture medium, the birch sap may be sterilized before being added to the culture medium, or the culture medium to which the birch sap has been added may itself be sterilized. Sterilization of the birch sap or a culture medium containing the birch sap can be carried out by any method, such as heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, or ozone sterilization. For example, the birch sap or a culture medium containing the birch sap may be heat sterilized at 65°C or higher, for example, 110-130°C or 120-130°C, and in one example, it can be sterilized by treating at 121°C for 20 minutes. After heat sterilization, it is preferable to cool the medium to at least a temperature suitable for yeast culture before using it for yeast culture.

Cultivation of yeast in a medium containing birch sap can be carried out by inoculating the medium with yeast and culturing it. Here, the medium may be a single composition of birch sap alone, or a mixed composition containing a normal yeast medium and birch sap. A normal yeast medium is a medium that contains the components necessary for culturing yeast (carbon source, nitrogen source, etc.). A normal yeast medium contains components selected from yeast extract, peptone, casein, soybean hydrolysate, malt extract, amino acids, etc. as the nitrogen source necessary for culturing. In addition, a normal yeast medium contains glucose, etc. as a carbon source, and mineral components such as sodium salt, potassium salt, calcium salt, and magnesium salt. In addition, when birch sap contains a sufficient amount of carbon source and/or mineral components for culturing yeast, the yeast medium used in combination with birch sap may have a composition in which the carbon source and/or mineral components are removed from the normal composition. In particular, a medium suitable for culturing yeast contains birch sap, yeast extract, and peptone.

When culturing yeast in a medium containing birch sap, the amount of sugar contained in the medium as a carbon source is preferably 0.05 to 5 g/100 ml in terms of glucose, more preferably 0.1 to 2 g/100 ml in terms of glucose. Here, birch sap contains about 1% by weight of sugar components such as glucose and xylose. Therefore, birch sap can be used as is without diluting or concentrating. By using birch sap as is, it is possible to fully exert its effects of maintaining and improving the condition of the skin, such as improving the texture and moisturizing the skin.

Birch sap may be diluted or concentrated to a specified ratio before use. By diluting or concentrating, the concentrations of the carbon source and mineral components contained in the birch sap can be adjusted to the desired range. It is also possible to add sugars such as glucose to the birch sap so that the carbon source is in the desired range. Similarly, mineral components can be added to the birch sap to achieve the desired concentration.

When culturing yeast in a medium containing birch sap, the yeast may be added (inoculated) directly to the medium, but it is preferable to add a pre-culture medium in which the yeast has been pre-cultured. By adding a pre-culture medium, the culture time for the main culture can be shortened and the risk of contamination can be reduced. The medium used for the pre-culture may have the same composition as the medium used for the main culture, or a normal yeast medium that does not contain birch sap may be used. In particular, it is most preferable to use a medium containing birch sap in this pre-culture to provide the carbon source and mineral components necessary for yeast culture.

By carrying out pre-culturing, the yeast can be maintained in the logarithmic growth phase while moving on to main culturing, and this can significantly improve the production efficiency in mass culturing of yeast. The conditions for culturing yeast are not particularly limited as long as they allow yeast to grow. The culture temperature is not particularly limited as long as it allows yeast to grow, but can typically be 15 to 45°C, preferably 25 to 40°C, for example 28 to 37°C. The pH during culture is not particularly limited as long as it allows yeast to grow, but can be 3.0 to 8.0, preferably 4.0 to 7.0, for example 5.0 to 7.0.

Yeast fungi are known to grow and proliferate well under aerobic conditions, as they can convert sugars into carbon dioxide and use it as an energy source. On the other hand, under oxygen-poor conditions, their growth slows down and they produce alcohol and carbon dioxide or organic acids through homo- or heterofermentation. In particular, when culturing yeast fungi in a medium containing birch sap, it is preferable to culture the yeast fungi under aerobic conditions. By culturing the yeast fungi under aerobic conditions, a culture solution that has effects of maintaining and improving the condition of the skin, such as improving the texture and moisturizing the skin, can be produced. To aerobically culture yeast fungi in a medium containing birch sap, an aeration and stirring type fermenter that is usually used for microbial culture can be used. The amount of aeration is not particularly limited, but can be, for example, 0.01 to 5 vvm, preferably 0.1 to 2 vvm, and more preferably 0.3 to 1.5 vvm. The stirring speed of the medium is not particularly limited, and conditions suitable for yeast fungi growth can be set arbitrarily.

In addition, during yeast cultivation, substances that change the pH, such as organic acids, may be produced, and the change in pH may inhibit yeast growth. For example, when organic acids accumulate during yeast cultivation, the pH of the medium will decrease, so it is preferable to continue cultivation by adding a pH adjuster to adjust the pH to within the above range, preferably pH 4.0 to 7.0, for example 5.0 to 7.0.

Furthermore, the time for culturing the yeast in the medium containing birch sap is not particularly limited, but can be, for example, 5 hours to 1 month, for example, 1 to 10 days or 1 to 5 days, preferably 1 to 3 days.

In this manner, yeast can be cultured in a medium containing birch sap to produce a culture solution. The resulting culture solution has excellent features such as maintaining and improving skin conditions, including skin texture improving effects and moisturizing effects. In particular, the resulting culture solution is extremely superior in terms of the degree to which it maintains and improves skin conditions, including skin texture improving effects and moisturizing effects, compared to the use of birch sap alone. Therefore, the resulting culture solution can be used as a skin topical agent or skin cosmetic.

In addition, the culture solution obtained by culturing yeast in a medium containing birch sap has a reduced sticky feeling compared to birch sap. Therefore, skin topicals and skin cosmetics containing this culture solution can maintain and improve skin conditions, such as improving skin texture and moisturizing effects, and also have a refreshing feel when used.

The obtained culture solution may be used as an external preparation for skin or a skin cosmetic as it is, or may be used as an external preparation for skin or a skin cosmetic after a process such as filtration. The obtained culture solution is not limited to a filtration process, and it is preferable to carry out a process of exuding bacterial components and components accumulated in the bacterial cells before the filtration process. This process is not particularly limited, but may be, for example, lysis by the action of digestive enzymes such as protease, lipase, amylase, etc., or the bacterial cells may be separated and then decomposed with acid, or the yeast may be physically crushed by means of high-speed rotary crushing, ultrasonic crushing, freeze-drying crushing, or the yeast may be heated for sterilization. These processes allow the useful components contained in the yeast to be rationally extracted into the culture solution.

As described above, the culture solution obtained by culturing yeast in a medium containing birch sap can be used as an external skin preparation or skin cosmetic as it is, but the culture solution that has undergone the various steps described above can also be used as an external skin preparation or skin cosmetic. Therefore, in this specification, the term "culture solution" includes not only the culture solution obtained by culturing yeast in a medium containing birch sap, but also the solution obtained after filtering the culture solution, the solution obtained after the various steps described above, the filtered solution, and solutions that have been subjected to one or more treatments such as centrifugation, deodorization and decolorization using filter aids such as activated carbon and diatomaceous earth, or a combination of these. In this specification, the "culture solution" also includes these solutions that have been concentrated, diluted, dried, or microfiltered, or those that have been purified by centrifugation or the like. The purification method is not particularly limited, but examples include filtration using a filter such as centrifugal filtration or a membrane filter.

In particular, as described above, it is preferable to heat the culture obtained by culturing yeast in a medium containing birch sap at high temperatures to exude useful components from the yeast cells into the culture. More specifically, the culture obtained is heated at 121°C for 20 minutes or more, and the resulting extract is centrifuged or filtered (e.g., microfiltration) or both to obtain a filtrate from which impurities, cells, insoluble matter, etc. have been removed. The exudation of useful components can be achieved by cell disruption using a high-pressure homogenizer, ultrasonic homogenizer, high-speed rotary homogenizer, etc., in addition to thermal cell decomposition, or by a combination of these methods. In addition, solid contents such as cell walls of the cells can be removed together with impurities and insoluble matter, etc., by filtration, for example, through a filter.

The obtained culture solution can be filtered by any method. For example, the filtration can be performed using a filter or other filtration device suitable for removing impurities, bacterial cells, and/or insoluble matter. Examples of filtration using a filter or other filtration device include centrifugal filtration, pressure filtration, and suction filtration. The filter used to filter the culture solution is not particularly limited, but examples include membrane filters, glass fiber filters, cellulose filters, and sintered filters. Filtration can be performed using one of these filters, or two or more types of filters in combination. More specifically, when a precision filtration filter is used, a filter having a pore size of typically less than 1 μm, preferably 0.5 μm or less, more preferably 0.1 μm to 0.5 μm or 0.2 μm to 0.5 μm, for example 0.45 μm, 0.22 μm, or 0.1 μm can be used. In addition, filtration using a precision filtration filter may be performed after filtration using diatomaceous earth filtration or a prefilter.

The obtained culture solution can be diluted or concentrated by any dilution or concentration method. When diluting, for example, water or any other solvent can be used. The culture solution can be concentrated by, for example, heat evaporation concentration method, freeze concentration method, ultrafiltration method, dialysis method, chromatography method, etc.

Any sterilization or disinfection method can be applied to the obtained culture solution. Examples of sterilization or disinfection methods include heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, and ozone sterilization.

When the obtained culture solution is dried to obtain a dried product, any drying method can be applied. Examples of the method for drying the culture solution include freeze-drying methods such as vacuum freeze-dried products, and thermal drying methods such as spray drying. The obtained culture solution can be deodorized and decolorized by any deodorizing and/or decolorizing method. Examples of the deodorizing and/or decolorizing method include a method in which the culture solution is passed through activated carbon to adsorb odorous components and attractants onto the activated carbon.

The culture solution obtained may be subjected to a wintering (dewaxing) process. That is, the culture solution obtained is cooled, components that solidify at low temperatures (wax) are precipitated, and the wax can be removed by solid-liquid separation using means such as filtration.

Here, topical skin preparations refer to pharmaceuticals containing ingredients (active substances) that are effective in diagnosing, treating, or preventing diseases in humans or animals, or bases used in such pharmaceuticals. Topical skin preparations include, for example, pharmaceuticals and quasi-drugs as defined in Japan's "Act on Ensuring Quality, Effectiveness, and Safety of Pharmaceuticals, Medical Devices, etc." On the other hand, skin cosmetics refer to cosmetics that are not classified as pharmaceuticals or quasi-drugs and are used on human skin. Topical skin preparations refer to cosmetics that are used on human skin as defined in Japan's "Act on Ensuring Quality, Effectiveness, and Safety of Pharmaceuticals, Medical Devices, etc."

Topical skin preparations may be in any dosage form, including, for example, ointments, patches, poultices, liniments, lotions, creams, extracts, liquid extracts, tinctures, elixirs, syrups, limonades, aromatic perfumes, liquids, suspensions, emulsions, medicated bath additives, medicated shampoos, aerosols, powders, granules, tablets, capsules, etc.

The skin topical preparation and skin cosmetic may be a composition in any form, such as a solution, suspension, emulsion, powder, paste, mousse, or gel. In particular, the skin cosmetic may be in any form that can be used by contacting the skin, specifically, for example, lotion, milky lotion, beauty essence, general cream (face cream, hand cream, body cream, etc.), face wash (cleansing cream, etc.), pack, shaving cream, suntan cream, sunscreen cream, sunscreen lotion, suntan lotion, cosmetic soap, foundation, face powder, powder, lipstick, lip balm, eyeliner, eye cream, eye shadow, mascara, bath additives (bath additives), shampoo, hair rinse, hair treatment, hair pack, scalp care agent, body rinse, body gel, hair dye, hair cosmetics, etc. Lotion, milky lotion, beauty essence, face cream, face wash, etc. are particularly preferred.

Furthermore, in skin topical preparations and skin cosmetics, the above-mentioned culture solution can be formulated in any form. For example, it may be simply mixed with other ingredients as is, or it may be formulated in the form of a gel, powder, granules, or encapsulated body, etc.

In addition to the above-mentioned culture solution, the skin cosmetic may contain additives acceptable for cosmetics, and other active ingredients, etc., as necessary. Such additives and other active ingredients, etc., may be selected as appropriate for the intended use and form. The skin cosmetic may be manufactured according to any cosmetic manufacturing method, for example, by mixing, stirring, molding, filling, etc., of the raw materials.

The skin topical preparation can be a composition containing the above-mentioned culture solution as a main agent that maintains and improves the skin condition, such as skin texture improving effect and moisturizing effect. Alternatively, the skin topical preparation can be a composition containing a predetermined medicinal ingredient (main agent) and the above-mentioned culture solution as a base that maintains and improves the skin condition, such as skin texture improving effect and moisturizing effect. The skin topical preparation can contain the above-mentioned culture solution, pharma- ceutical acceptable additives, and other active ingredients as necessary. Such additives, other active ingredients, etc. can be selected arbitrarily and used according to the purpose and form of the pharmaceutical. The pharmaceutical according to the present invention can be manufactured by mixing, stirring, molding, filling, etc. of raw materials according to any pharmaceutical manufacturing method.

Skin topical preparations and skin cosmetics may contain one or more additives for formulations, such as carriers (solid or liquid carriers), excipients, surfactants, binders, disintegrants, lubricants, odorants, solubilizers, suspending agents, coating agents, pH adjusters, colorants, fragrances, flavorings, cooling agents, stabilizers, foaming agents, preservatives, and buffers.

The skin topical preparation and skin cosmetic preparation may contain, for example, one or more components selected from any of the following groups. Any component selected from proteins and protein hydrolysates, such as collagen, collagen hydrolysate, gelatin, gelatin hydrolysate, elastin, elastin hydrolysate, lactoferrin, keratin, keratin hydrolysate, casein, albumin, royal jelly-derived protein hydrolysate, and honey-derived protein hydrolysate, may be included. Any component selected from natural polymers and derivatives thereof, such as sodium hyaluronate and hyaluronic acid derivatives, xanthan gum, carrageenan, guar gum, alginic acid and its salts, pectin, chondroitin sulfate and its salts, water-soluble chitin, chitosan derivatives and its salts, pullulan, deoxyribonucleic acid, gum arabic, and gum tragacanth, may be included. Any component selected from herbal medicines, such as ground cherries, monk fruit, jujube, pearl barley, licorice, eucommia, ginger, and udo. It may contain any component selected from seaweed powder, seaweed extract, and polysaccharides derived from seaweed. It may contain any component selected from animal-derived substances such as placenta extract. It may contain any component selected from water such as carbonated water, hot spring water, micro-nano bubble water, purified water, and distilled water. It may contain any component selected from acidic polymers such as carboxyvinyl polymers and their salts, polyacrylic acid and its salts, carboxymethyl cellulose and its salts, and neutral polymers such as polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinyl pyrrolidone, and nitrocellulose polyvinyl methyl ether. It may contain any component selected from cationic polymers such as cationized cellulose, polyethyleneimine, and cationized guar gum. It may contain any component selected from lower alcohols such as ethanol and isopropyl alcohol. It may contain any component selected from ultraviolet absorbers such as para-aminobenzoic acid-based ultraviolet absorbers, salicylic acid-based ultraviolet absorbers, cinnamic acid-based ultraviolet absorbers, anthranilate-based ultraviolet absorbers, and benzophenone-based ultraviolet absorbers. It may contain any component selected from anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin. It may contain any component selected from antioxidants such as stearic acid esters, nordihydroguaiaretenic acid, dibutyl hydroxytoluene, butyl hydroxyanisole, parahydroxyanisole, propyl gallate, sesamol, sesamolin, and gossypol. It may contain any component selected from parabenzoic acid esters such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate, and butyl parabenzoate, and preservatives such as sorbic acid, dehydroacetic acid, phenoxyethanol, and benzoic acid. It may contain any component selected from edetic acid and its salts such as edetic acid and disodium edetate, phytic acid, and hydroxyethane disulfonic acid, and sequestering agents. It may contain any component selected from polyhydric alcohols such as glycerin, 1,3-butylene glycol, and propylene glycol. The composition may contain any component selected from amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, and glycine, and salts thereof. The composition may contain any component selected from sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, and honey. The composition may contain any component selected from bases such as ammonia water, monoethanolamine, triethanolamine, sodium hydroxide, and potassium hydroxide. The composition may contain any component selected from hydrocarbons such as liquid paraffin, squalane, and vaseline. The composition may contain any component selected from fats and oils such as coconut oil, evening primrose oil, jojoba oil, castor oil, and hydrogenated castor oil. The composition may contain any component selected from fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and behenic acid. The composition may contain any component selected from higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, and behenyl alcohol. It may contain any component selected from esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glycerin trioctanoate, octyldodecyl myristate, octyl stearate, and stearyl stearate. It may contain any component selected from phospholipids such as lecithin and its derivatives. It may contain any component selected from lipids derived from animals and plants such as bovine bone marrow fat and bovine brain lipid. It may contain any component selected from alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate. It may contain any component selected from polyoxyethylene alkyl sulfates such as polyoxyethylene (2EO) lauryl ether sulfate triethanolamine (EO is ethylene oxide, and the number before EO indicates the number of moles of ethylene oxide added; the same applies below) and polyoxyethylene (3EO) alkyl (one of 11 to 15 carbon atoms or a mixture of two or more) ether sulfate sodium. It may contain any component selected from alkylbenzene sulfonates such as sodium laurylbenzene sulfonate and triethanolamine laurylbenzene sulfonate. It may contain any component selected from polyoxyethylene alkyl ether sulfates such as sodium polyoxyethylene (3EO) tridecyl ether acetate. It may contain any component selected from N-acyl amino acid salts such as sodium coconut oil fatty acid sarcosine, triethanolamine lauroyl sarcosine, sodium lauroyl methyl-L-glutamate, sodium coconut oil fatty acid-L-glutamate, triethanolamine coconut oil fatty acid-L-glutamate, sodium coconut oil fatty acid methyl taurate, and sodium lauroyl methyl taurate, sodium ether sulfate alkane sulfonate, sodium hydrogenated coconut oil fatty acid glycerin sulfate, sodium hydrogenated coconut oil fatty acid glycerin sulfate, disodium undecynoyl amidoethyl sulfosuccinate, sodium octylphenoxy diethyl oxyethyl sulfonate, oleic acid glycerin sulfate, sodium ... The surfactant may include any component selected from anionic surfactants such as disodium aminosulfosuccinate, dioctyl sodium sulfosuccinate, dioctyl sodium sulfosuccinate, disodium lauryl sulfosuccinate, polyoxyethylene alkyl (12-15 carbon chain) ether phosphate (8-10EO), sodium polyoxyethylene oleyl ether phosphate, sodium polyoxyethylene cetyl ether phosphate, disodium polyoxyethylene lauryl sulfosuccinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, and sodium tetradecene sulfonate. The surfactant may include any component selected from cationic surfactants such as stearyl dimethyl ammonium chloride, di(polyoxyethylene)oleyl methyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, stearyl trimethyl ammonium chloride, tri(polyoxyethylene)stearyl ammonium chloride, polyoxypropylene methyl diethyl ammonium chloride, myristyl dimethyl benzyl ammonium chloride, and lauryl trimethyl ammonium chloride. The surfactant may include any component selected from amphoteric surfactants such as 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, sodium undecyl hydroxyethyl imidazolinium betaine, undecyl-N-hydroxyethyl-N-carboxymethyl imidazolinium betaine, stearyl dihydroxyethyl betaine, coconut oil fatty acid amidopropyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethyl imidazolinium disodium lauryl sulfate, and N-coconut oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate. The surfactant may contain any component selected from nonionic surfactants such as polyoxyethylene alkyl (carbon number 12 to 14) ether (7EO), polyoxyethylene octylphenyl ether, polyoxyethylene oleyl ether, polyoxyethylene glycerin oleate, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxyethylene cetylstearyl diether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyl tetradecyl ether, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol, and polyoxypropylene stearyl ether. It may contain any component selected from thickeners such as isostearic acid diethanolamide, undecylenic acid monoethanolamide, oleic acid diethanolamide, beef tallow fatty acid monoethanolamide, hardened beef tallow fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristic acid diethanolamide, coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, and lanolin fatty acid diethanolamide. It may contain any component selected from chain or cyclic methyl polysiloxane, methylphenyl polysiloxane, dimethyl polysiloxane polyethylene glycol copolymer, dimethyl polysiloxane polypropylene copolymer, amino-modified silicone oil, and silicone oil such as quaternary ammonium-modified silicone oil. It may contain any component selected from thioglycolic acid and its salts. It may contain any component selected from cysteamine and its salts. It may contain any component selected from peroxides such as hydrogen peroxide, persulfates, perborates, and urea peroxide. It may contain any ingredient selected from bromates such as sodium bromate and potassium bromate. It may contain any ingredient selected from other active ingredients such as keratolytic agents, astringents, wound healing agents, deodorants, antiallergic agents, blood flow promoters, and cell activators.

By the way, since the skin topical preparation and skin cosmetic contain the above-mentioned culture solution, they can maintain and improve the skin condition, such as the skin texture improving effect and moisturizing effect. Here, maintaining and improving the skin condition means maintaining and/or improving the moisturizing property of the skin, maintaining and/or improving the skin texture, and preventing and/or improving dullness of the skin. Specifically, the skin texture improving effect is the effect of improving the skin to a fine texture, that is, a state in which the skin grooves on the skin surface are shallow and each skin ridge is clearly shaped as a small, beautiful triangle. In addition, the dullness prevention effect is the effect of acting on the skin parts that are lower in brightness compared to the surrounding area, known as dull skin, narrowing the area of dull skin and reducing the degree of decrease in brightness.

The skin topical preparations and skin cosmetics are particularly useful when administered or applied to subjects with at least one of the symptoms of dry skin, dull skin, and rough skin. Examples of such subjects include subjects with factors that make them prone to at least one of dry skin, dull skin, and rough skin. Factors that make them prone to at least one of dry skin, dull skin, and rough skin include subjects with the environment, lifestyle habits, dietary habits, genetic factors, constitution, disease, mental and physical stress, aging, and the like. By applying the skin topical preparations and skin cosmetics to subjects with these factors, at least one of dry skin, dull skin, and rough skin can be improved, and health or beauty benefits can be expected.

The dosage and application amount of topical skin preparations and skin cosmetics vary depending on the age, body weight, application/administration route, dosage form, number of administrations, etc. of the subject, and can be changed widely at the discretion of a person skilled in the art. Specifically, the application amount or administration amount is not particularly limited, but can be, for example, 0.001 mg to 10 g per administration, converted into the dry weight of the culture medium, which is the active ingredient. Topical skin preparations and skin cosmetics may be repeatedly administered or applied at intervals of, for example, 6 to 24 hours, but are not limited thereto.

The present invention will be described in more detail below with reference to examples, but the technical scope of the present invention is not limited to the following examples.

[Example 1] Preparation of sap yeast culture solution and control product All birch sap used in this example was produced in Hokkaido and was filtered from the original solution without any processing such as concentration or dilution.

<Production Example 1> Galactomyces reessii
A pre-culture medium was prepared by using birch sap from Hokkaido as the substrate for pre-culture, and adding yeast extract and peptone as supplementary nutrients (see Table 1). The pre-culture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the pre-culture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Production Example 1, the pre-culture medium was used for shaking pre-culture at 30°C for 48 hours.

For the main culture, birch sap (2000 g) roughly filtered using a cartridge filter with 20 μm mesh was placed in a small jar fermenter for microbial culture and sterilized at 121°C for 20 minutes. After cooling the birch sap to 30°C, 50 g of preculture medium was added, and Galactomyces raceii was cultured at 30°C while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. During the culture period, samples were taken at appropriate times, and the glucose concentration in the culture solution was measured using an enzymatic method (F Kit Glucose: JK International Co., Ltd.). The glucose content in the culture solution is shown in Figure 1. As shown in Figure 1, glucose was consumed and decreased as the bacteria grew, and the glucose level, which was initially 1200 ppm, fell to 20 ppm or less at the end of the culture.

The resulting culture solution was heated at 121°C for 30 minutes to extract the bacterial components, and then cooled to room temperature. The resulting solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45 μm membrane filter to remove the solids, yielding a birch sap Galactomyces culture solution.

<Production Example 2> Saccharomycopsis capsularis
In this Production Example 2, except that Saccharomycopsis capsularis was used, a birch sap Saccharomycopsis culture solution was obtained in the same manner as in Production Example 1. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

<Production Example 3> Saccharomyces cerevisiae
In this Production Example 3, except that Saccharomyces cerevisiae was used, a birch sap Saccharomyces culture solution was obtained in the same manner as in Production Example 1. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

<Production Example 4> Cultivation with multiple bacteria In this Production Example 4, a birch sap Galactomyces-Saccharomyces culture was obtained in the same manner as in Production Example 1, except that Galactomyces reessii and Saccharomyces cerevisiae were used. The change in glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1). In addition, in Production Example 4, the birch sap Galactomyces-Saccharomyces culture obtained was vacuum freeze-dried to obtain a freeze-dried powder of birch sap Galactomyces-Saccharomyces culture.

Comparative Example 1: Uncultured birch sap In Comparative Example 1, birch sap (2000 g) roughly filtered using a cartridge filter with a mesh size of 20 μm was sterilized at 121° C. for 20 minutes. The sterilized birch sap was cooled to room temperature and filtered through a prefilter and then a 0.45 μm membrane filter to prepare a sterilized birch sap filtrate. The glucose concentration of this sap was measured using an enzymatic method (F Kit Glucose: JK International Co., Ltd.).

Comparative Example 2: Yeast culture in skim milk medium In Comparative Example 2, skim milk medium was prepared as a substrate for preculture (see Table 1). The prepared preculture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the preculture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Comparative Example 2, shaking culture was performed at 30°C for 48 hours using this preculture medium.

For the main culture, 20 g of skim milk, 10 g each of glucose and yeast extract were dissolved in 1960 g of purified water to prepare the main culture medium, which was placed in a small jar fermenter for microbial culture and sterilized at 121°C for 20 minutes. After the main culture medium was cooled to 30°C, 50 g of preculture medium was added, and Galactomyces raceii was cultured at 30°C for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The resulting skim milk Galactomyces culture solution was heat-treated at 121°C for 30 minutes to extract the bacterial components, and then cooled to room temperature. The resulting solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45 μm membrane filter to obtain a skim milk Galactomyces culture solution from which solids had been removed.

Comparative Example 3: Yeast culture in soy milk medium In Comparative Example 3, soy milk medium was prepared as a substrate for preculture (see Table 1). The prepared preculture medium was placed in a baffled flask, covered with silicone, and sterilized at 121°C for 20 minutes. After sterilization, the preculture medium was cooled to 30°C, and one loopful of Galactomyces reessii was inoculated. In this Comparative Example 3, shaking culture was performed at 30°C for 48 hours using this preculture medium.

For the main culture, 2000 g of soy milk was placed in a small jar fermenter for microbial culture and sterilized at 121°C for 20 minutes. After the main culture medium was cooled to 30°C, 50 g of preculture was added, and Galactomyces raceii was cultured at 30°C for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The resulting soy milk Galactomyces culture solution was heat-treated at 121°C for 30 minutes to extract the bacterial components, and then cooled to room temperature. The resulting solution containing solids such as yeast cell walls was filtered through a prefilter and then a 0.45 μm membrane filter to obtain a soy milk Galactomyces culture solution from which the solids had been removed.

Example 2 The following describes the evaluation test methods and results using the various culture fluids produced in Example 1 and the culture fluids of the comparative examples. A list of the samples used for the evaluation is shown in Table 2.

Example 2: Evaluation of moisturizing properties based on stratum corneum moisture content and moisture evaporation rate A lotion with the composition shown in Table 3 was prepared using the samples shown in Table 2 and applied to the inner forearms of 10 female subjects in their 40s to 50s twice a day for 4 weeks. The moisture content of the stratum corneum was measured using a Corneometer (Courage + Khazaka) before application and 2 and 4 weeks after application, and the transepidermal moisture evaporation rate was measured using a Tewameter (Courage + Khazaka electronic GmbH). The results are shown in Tables 4 and 5.

As shown in Table 4, an increase in stratum corneum moisture content was observed in test plots 1-4 and control plots 1-3 compared to Comparative Example 4, which used purified water. In particular, all test plots 1-4, in which yeast was cultured using birch sap as the substrate, showed an extremely excellent effect on stratum corneum moisture content retention, and birch sap without yeast culture (control plot 1), culture medium with skim milk as substrate (control plot 2), and culture medium with soy milk as substrate (control plot 3) also showed some moisturizing effect, but the results were inferior to those of test plots 1-4. In addition, when comparing by type of yeast, it was confirmed that the culture medium with Galactomyces raceii (test plot 1) and culture medium with Saccharomycopsis (test plot 2) showed a high effect on stratum corneum moisture retention.

As shown in Table 5, the amount of water evaporation was increased in control area 2 (purified water) during the test period due to environmental influences, but the other test areas and control areas were confirmed to have a transepidermal water evaporation inhibitory effect. In particular, all test areas 1 to 4, in which yeast was cultured using birch sap as the substrate, had an extremely excellent water evaporation inhibitory effect. Although some water evaporation inhibitory effect was observed in birch sap without yeast culture (control area 1), culture medium with skim milk as the substrate (control area 2), and culture medium with soy milk as the substrate (control area 3), the results were inferior to those of test areas 1 to 4. In addition, when comparing the types of yeast, it was confirmed that the water retention and evaporation inhibitory effect was high in two test areas: birch sap yeast culture medium with Galactomyces raceii selected (test area 1) and birch sap yeast culture medium with Saccharomycopsis selected (test area 2).

[Example 3] Skin Texture Improvement Test 1
The samples shown in Table 3 were applied twice a day for four weeks to ten female subjects in their 40s and 50s, marking an area of 15 mm x 15 mm on the inner forearm of each test area. The skin texture before and after application was photographed using a microscope (Keyence Corporation) and compared. The images were evaluated on the following three items (depth of skin groove, shape of skin mound, size of skin mound) using the following five-point scale, and the average scores of the ten subjects are shown in Tables 6 to 8.

(1) Depth of the skin groove: 5 Very shallow skin grooves 4 Slightly shallow skin grooves 3 Normal skin grooves 2 Slightly deep skin grooves 1 Very deep skin grooves (2) Shape of the skin mound: 5 All are perfect triangles 4 Almost triangular.
3. Skin ridges other than the triangle are slightly noticeable. 2. Skin ridges other than the triangle are noticeable. 1. Skin ridges other than the triangle are very noticeable. (3) Size of the skin ridges: 5. Very small and well-formed. 4. Slightly small. 3. Medium size. 2. Slightly large. 1. Very large.

As shown in Tables 6 to 8, compared to Comparative Example 4, which used purified water, the skin texture improving effect was confirmed in Test Areas 1 to 4 and Control Areas 1 to 3. In particular, the skin texture improving effect was extremely excellent in all Test Areas 1 to 4, in which yeast was cultured using birch sap as the substrate component. Although some skin texture improving effect was observed in birch sap without yeast culture (Control Area 1), culture solution with skim milk as substrate (Control Area 2), and culture solution with soy milk as substrate (Control Area 3), the results were inferior to those of Test Areas 1 to 4. When comparing by type of yeast, it was confirmed that the birch sap yeast culture solution (Test Area 1), which selected Galactomyces raceii, had a high skin texture improving effect.

[Example 4] Stickiness evaluation test 1
In this Example 4, in the second week of the continuous application test period in Example 2, the stickiness of the application site of each test group before and after drying for 15 minutes was measured and compared using a skin friction force evaluation device, Frictiometer FR700 (manufactured by Courage+Khazaka Co., Ltd.) before and after lotion application. The friction force value before application was set to 100, and the value after application was calculated and averaged for 10 subjects, and is shown in Table 9.

As shown in Table 9, in control area 1, stickiness due to the sugars contained in it was observed, and the frictional force was also very high. In other words, it was made clear that birch sap itself causes a sticky feeling. In addition, the yeast culture fluids in control areas 2-3, which used skim milk and soy milk as substrates, also showed high frictional force and were sticky, although not as strong as those in control area 1. In comparison, test areas 1-4, in which yeast was cultured using birch sap as a substrate, showed values close to that of purified water in control area 4, indicating that they provide an excellent feel without causing a sticky feeling.

[Example 5] Skin Texture Improvement Test 2
In this Example 5, the samples shown in Table 10 were applied twice a day for 4 weeks to the left and right cheeks of 10 female subjects in their 40s to 50s. The right cheek was used as the test group and the left cheek was used as the control group. The skin texture before and after application was measured using a skin image analyzer (VISIA™ Evolution, Canfield). The skin texture evaluation was quantified and the results are shown in Table 11.

As shown in Table 11, the study in this example also confirmed that test area 1, in which Galactomyces was cultured using birch sap as a substrate component, had a greater effect on improving texture than the control area, in which birch sap itself was used.

[Example 6] Dullness Improvement Test In this Example 6, in parallel with the test in Example 5, the dullness improvement effect was measured in the test section and the control section. Dullness was evaluated using a colorimeter (Konica Minolta), which is a color measuring device, and the lightness index L* value in the L*a*b* color system was obtained and analyzed. The results before application and after 4 weeks of continuous application are shown in Table 12 (in the table, the L* value is the average value of the subjects (10 people)).

As shown in Table 12, test area 1, in which Galactomyces was cultivated using birch sap as a substrate component, was confirmed to have a greater effect on reducing dullness than the control area, in which birch sap itself was used.

[Example 7] Stickiness evaluation test 2 (sensory test)
In this Example 7, a sensory test was conducted on the sticky feeling during the tests of Examples 5 and 6. Specifically, the evaluation was conducted every week from one week to four weeks after the start of the test, using the following five-level rating system. The average scores of the 10 subjects are shown in Table 13.
Skin stickiness rating: 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky

As shown in Table 13, the test area in which Galactomyces was cultivated using birch sap as the substrate component gave a refreshing feel throughout the entire period, while the control area in which birch sap itself was used gave a sticky feel due to the sugars in the sap.

[Example 8] Example of production of lotion In this Example 8, lotion (lotion containing birch sap yeast culture fluid) was produced by stirring components (1) to (11) at 80°C according to the recipe in Table 14, dissolving them, and then cooling to room temperature. In addition, a control lotion was produced in exactly the same manner as in Comparative Example 1, except that the yeast-cultured birch sap prepared in Production Example 1 was not added. All of the obtained lotions were clear, and were stable for 3 months at 40°C and a relative humidity (RH) of 75% without developing any cloudiness.

A sensory test was conducted on these lotions by 10 female subjects in their 40s and 50s. Evaluation was carried out on the following 5-point scale for 4 items (skin moistness, skin smoothness, skin stickiness, and sensory irritation). The average scores of the subjects (10 subjects) are shown in Table 15.

(1) Skin moisturization 5: Moist 4: Slightly moist 3: Normal 2: Slightly dry 1: Dry
(2) Skin smoothness 5: Smooth 4: Slightly smooth 3: Normal 2: Slightly rough 1: Rough
(3) Skin stickiness 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky
(4) Sensory stimulation 5: No particular stimulation felt.
4: There is some irritation, but it doesn't bother me.
3: Slight tingling, burning, prickling, itching, or other irritation is felt, but is tolerable.
2: Strong irritation such as tingling, burning, prickling, and itching.
1: Very strong irritation such as tingling, burning, prickling, and itching.
0: Severe stimulation, cannot continue testing

As shown in Table 15, the lotion containing the culture solution of Galactomyces cultured using birch sap as a substrate component exhibited superior moisturizing properties and reduced stickiness compared to the control lotions 1 and 2.

Example 9 Production of a Cream In this Example 9, a mixture obtained by mixing and stirring components (1) to (7) at 80°C according to the formulation shown in Table 16 was added to a mixture obtained by mixing and stirring components (8) to (11) at 80°C separately, followed by homogenization and cooling to room temperature with stirring to produce a cream.

The resulting cream was not sticky during use and left the skin moisturized.

[Example 10] Preparation of body gel In Example 10, a body gel was prepared by stirring and dissolving the components (1) to (7) according to the recipe in Table 17. The obtained body gel moisturized the skin and was a non-irritating preparation.

Example 11: Production of shampoo In Example 11, components (1) and (2) were dissolved by heating according to the recipe in Table 18, and components (3) and (4) heated to 60°C were added and stirred. Components (5) to (8) were added and stirred. Component (9) was added to adjust the pH to 5.5 to 6.0, and further components (11) and (12) dissolved in component (10) were added. Component (13) was added until the total amount (100%) was reached to produce a shampoo.

When the resulting shampoo was used to wash the hair, it left the hair feeling smooth and moisturized.

[Example 12] Production of cleansing cream In Example 12, a cleansing composition was obtained by homogeneously mixing all ingredients by stirring according to the formulation in Table 19. The obtained cleansing cream had good cleansing properties and provided a refreshing feeling after use.

[Example 13] Preparation of rinse agent In Example 13, a rinse composition was obtained by homogeneously mixing all components by stirring according to the formulation in Table 20. The obtained rinse gave a moist feeling to hair and scalp after use.

[Example 14] Preparation of emulsion In Example 14, an emulsion composition was obtained by homogeneously mixing all ingredients by stirring according to the formulation in Table 21. The obtained emulsion provided a moist and hydrated feeling to the skin.

[Example 15] Production of mask In Example 15, a mask was produced by impregnating a nonwoven fabric with a skin topical preparation having the composition shown in Table 22. The produced mask was used to cover the face for 15 minutes, and provided a suitable level of moisture and firmness after use.

Claims (6)

A culture medium in which a yeast belonging to the genus Galactomyces and/or a yeast belonging to the genus Saccharomycopsis is grown in a medium containing birch sap. The culture medium according to claim 1, characterized in that the birch sap is sap obtained from Betula platyphylla. A skin external preparation comprising the culture medium according to claim 1 or 2 . A skin cosmetic comprising the culture medium according to claim 1 or 2 . 5. The skin cosmetic composition according to claim 4 , which exhibits at least one effect selected from the group consisting of an effect of improving moisturizing properties, an effect of improving skin texture, and an effect of reducing dullness. A method for producing a culture medium, characterized in that during the process of culturing a yeast belonging to the genus Galactomyces and/or a yeast belonging to the genus Saccharomycopsis in a medium containing birch sap, low molecular weight sugars in the birch sap are consumed as a nutrient source for the fungi , resulting in a lower concentration of low molecular weight sugars in the birch sap compared to before the start of the culture.

Images