JP6927709B2 - Epidermis normalizing agent and external preparations and cosmetics containing it - Google Patents

Description

The present invention relates to an epidermal normalizing agent that enhances the expression of a cornified envelope-forming factor in epidermal keratinized cells, and an external preparation and a cosmetic containing the epidermal normalizing agent.

The skin is the largest organ in the human body with a three-layer structure consisting of the epidermis, dermis and subcutaneous tissue, and has a barrier function that protects internal cells from external stimuli and a function that prevents internal water loss. Moisture is important for the skin that carries out these functions, and when the skin becomes dry due to lack of water, the barrier function deteriorates, resulting in rough skin, itchiness, wrinkles and inflammation.

The epidermis is composed of a stratum corneum, a stratum granulosum, a stratum spinosum, and a stratum basale. The stratum corneum located on the outermost surface prevents irritation from the outside world and plays an important role in the barrier function of the skin. It is known that the barrier function of the skin is closely related to the tough insolubilized protein membrane formed during the transition from the stratum granulosum to the stratum corneum, that is, the cornified envelope. The cornified envelope is also considered to provide a foothold for the crystal structure of intercellular lipids, and it can be said that promoting the formation of the cornified envelope is important for the expression of the skin barrier function.

Involucrin and the transglutaminase that crosslinks it are involved in the formation of the cornified envelope. When the amount of involucrin decreases, the barrier function is greatly reduced, which contributes to incomplete keratinization, and when the amount of transglutaminase is decreased, keratinization is not performed normally, which affects the deterioration of the barrier function.
In addition, since it is speculated that the amount of involucrin and transglutaminase decreases with aging and is related to skin aging, it is considered that the skin condition becomes more favorable by promoting the production of involucrin and transglutaminase.

Conventionally, eucalyptus is known as a cornified envelope formation promoter (Patent Document 1). As involucrin production promoters, kelp, choy sum and rafuma are known (Patent Documents 2 and 3). Choy sum and Rafuma are known as transglutaminase production promoters (Patent Document 3). There are known methods for improving rough skin and the like with plant extracts such as chimpi extract, rosemary extract and prune extract (Patent Documents 4 to 6).
However, in order to improve the barrier function of the skin by using these plant extracts, it is necessary to increase the amount of the plant extract used, and even if the amount used is increased, the improvement effect is often not seen. Improving the improvement effect has become an issue.

Japanese Unexamined Patent Publication No. 2001-288066 Japanese Unexamined Patent Publication No. 2009-84195 Japanese Unexamined Patent Publication No. 2007-1914 Japanese Unexamined Patent Publication No. 2001-81037 Japanese Unexamined Patent Publication No. 06-24937 Japanese Unexamined Patent Publication No. 09-2967

An object of the present invention is to provide an epidermal normalizing agent that enhances the expression of a cornified envelope-forming factor in epidermal keratinized cells and a cosmetic using the same.

As a result of intensive studies to solve the above problems, the present inventors have found that the effect of promoting the formation of a cornified envelope is dramatically increased by a combination of specific plant extracts, and based on this finding, the present inventions. Has been completed.
That is, the present invention comprises a cornified envelope formation promoter containing at least one plant extract selected from the group consisting of sage extract, kagosou extract, majorum extract, keigai extract, rensensou extract, peppermint extract and spearmint extract, and a chimpi extract. , A skin normalizing agent containing at least one plant extract selected from the group consisting of rosemary extract and prune extract.

Furthermore, the present invention comprises a cornified envelope formation promoter containing a sage extract.
Provided is an epidermis normalizing agent containing at least one plant extract selected from the group consisting of chimpi extract, rosemary extract and prune extract.
In particular, the present invention comprises a cornified envelope formation promoter containing a sage extract.
Provided is an epidermis normalizing agent containing a mixture of a plant extract consisting of a chimpi extract, a rosemary extract and a prune extract.

According to the present invention, it is possible to provide an epidermis normalizing agent that synergistically improves the effect of promoting the formation of a cornified envelope, which can be expected to have an effect of improving the barrier function of the skin.

In the present invention, "normalizing the epidermis" refers to the effect of promoting the formation of the cornified envelope, and the effect of promoting the formation of the cornified envelope refers to the effect of promoting the production of involucrin and the production of transglutaminase.
The epidermis normalizing agent of the present invention is a cornified envelope formation promoter containing at least one plant extract selected from the group consisting of sage extract, Kagosou extract, Majorum extract, Keigai extract, Rensensou extract, peppermint extract and Sparemint extract. And at least one plant extract selected from the group consisting of chimpi extract, rosemary extract and prune extract.
All of these are plant extracts or purified components thereof, and are generally used as components of pharmaceuticals or folk medicines, foods, and cosmetics.

The sage extract is a plant extract extracted from the leaves of sage Salvia officinalis, which is a kind of plant belonging to the genus Sage of the Labiatae family, but plants of the same genus can also be used.
The Kagosou extract is a plant extract extracted from the flower ears of Kagosou Prunella vulgaris, which is a kind of plant belonging to the genus Heal-all of the Labiatae family, but plants of the same genus can also be used.
The marjoram extract is a plant extract extracted from the leaves of marjoram Oregano majora, which is a kind of plant belonging to the genus Oregano of the Labiatae family, but plants of the same genus can also be used.
The schizonepeta extract is a plant extract extracted from the whole plant of the schizonepeta schizonepeta tenuifolia, which is a kind of plant of the genus Catnips of the Labiatae family, but plants of the same genus can also be used.

The ground-ivy extract is a plant extract extracted from the whole plant of Glechoma hederacea, which is a kind of plant of the genus Glechoma of the Labiatae family, but plants of the same genus can also be used.
The peppermint extract is a plant extract extracted from the whole plant of peppermint Mentha piperita, which is a kind of plant belonging to the genus Peppermint of the Labiatae family, but plants of the same genus can also be used.
The spearmint extract is a plant extract extracted from the whole plant of spearmint Mentha spicata, which is a kind of plant belonging to the genus Mentha of the Labiatae family, but plants of the same genus can also be used.
The chimpi extract is a plant extract extracted from the peel of Citrus unshiu, which is a kind of plant belonging to the genus Citrus of the Rutaceae family, but plants of the same genus can also be used.
The rosemary extract is a plant extract extracted from the whole plant of rosemary Rosmarinus officinalis, which is a kind of plant belonging to the genus Rosmarinus of the Labiatae family, but plants of the same genus can also be used.
The prune extract is a plant extract extracted from the fruit of prune Prunus domestica, which is a kind of plants belonging to the genus Plum of the Rosaceae family, but plants of the same genus can also be used.

The sage extract, Kagosou extract, Majorum extract, Keigai extract, Rensensou extract, Peppermint extract, Sparemint extract, Chinpi extract, Rosemary extract, and Prune extract used in the present invention are solvent-extracted from various parts of each of the plants or crushed products thereof. It can be obtained by supercritical extraction or a method of decomposing and extracting a plant with an enzyme or the like. Moreover, each commercially available plant extract may be used.

In the extraction of the plant extract, the extraction solvent that can be used is not particularly limited, and lower (1 to 4 carbon atoms) monohydric alcohols such as water, methanol, ethanol, propanol and butanol, propylene glycol, dipropylene glycol and butylene glycol are used. , Polyhydric alcohols such as glycerin, esters such as methyl acetate and ethyl acetate, ketones such as acetone and methyl ethyl ketone, ethers such as diethyl ether and tetrahydrofuran, and polar solvents; halogenation of chloroform, carbon tetrachloride and the like. Hydrocarbons such as hydrocarbons, hexane, heptane, and cyclohexane can be used, and one of these solvents may be used alone, or a mixed solvent consisting of two or more kinds may be used. Among these solvents, a polar solvent can be particularly preferably used.

The extraction method by immersing the plant extract is not particularly limited, and examples thereof include a method in which the raw material of the plant extract is immersed in the solvent at 20 to 40 ° C. for 1 hour to 7 days and then filtered.
The supercritical fluid used in the supercritical extraction method is not particularly limited, and examples thereof include water and carbon dioxide.
The extraction method by decomposition is not particularly limited, and for example, fruits and the like are put in a solvent, enzymes (for example, fibrinolytic enzymes), microorganisms, acids, alkalis and the like are added thereto, and the mixture is reacted for 1 hour to 7 days to be decomposed. , The reaction is stopped by heating or neutralization, and filtration can be mentioned. Examples of the solvent include the extraction solvent, and water is preferably used.

The raw material of the plant extract is preferably dried by subjecting it to a drying treatment such as hot air drying, vacuum drying, spray drying or freeze drying, and it is more preferable to use a dried pulverized product. Further, the mixture may be stirred or allowed to stand while being immersed in the extraction solvent. The plant extract of the present invention can also be obtained as a high-concentration plant extract by repeating the extraction operation.
Each of these plant extracts may be used as an extract or a decomposition solution dissolved in an extraction solvent or a decomposition solvent, or may be concentrated or diluted, and may be treated by concentrated drying, spray drying, freeze drying, or the like. May be used as a dried product.

In the epidermis normalizing agent of the present invention, the mass ratio of the total of sage extract, kagosou extract, majorum extract, keigai extract, rensensou extract, peppermint extract and spearmint extract to the total mass ratio of chimpi extract, rosemary extract and prune extract is It is preferably 50: 1 to 1:50, more preferably 20: 1 to 1:20 in terms of the dried product of the plant extract. When the mass ratio is in this range, the effect of normalizing the epidermis, that is, the effect of promoting the formation of the cornified envelope becomes more excellent.
The epidermis normalizing agent of the present invention can be produced by mixing each plant extract as it is in a predetermined amount, and may further contain a solvent and other additive components such as a preservative.

When a solvent or other additive components are blended, the blending amount of the plant extract according to the present invention in the skin normalizing agent of the present invention is preferably 0.00001% by mass or more in total, more preferably 0.00001% by mass or more in terms of dry matter. , A total of 0.00002 mass% or more, particularly preferably a total of 0.0002 mass% or more.
In particular, when one or more plant extracts selected from sage extract, kagosou extract, marjoram extract and keigai extract are used as the cornified envelope formation promoter, the blending amount of the plant extract is higher in terms of dry matter. It is preferable that the total amount is 0.00001% by mass or more, and particularly preferably the total amount is 0.0001% by mass or more. Further, when one or more plant extracts selected from Rensensou extract, peppermint extract and spearmint extract are used as the cornified envelope formation promoter, the blending amount of the plant extract is more preferably in terms of dry matter. , A total of 0.0001% by mass or more, particularly preferably a total of 0.001% by mass or more.

Further, the blending amount of one or more plant extracts selected from the chimpi extract, rosemary extract and prune extract is the mass ratio to the total amount of various plant extracts as a cornified envelope formation promoter in terms of dry matter. It is preferable to adjust the total amount, and it is more preferable that the total amount is 0.00001% by mass or more.

The epidermis normalizing agent of the present invention can be blended in various external preparations such as cosmetics for skin or hair, medicinal external preparations, quasi-drugs, and pharmaceuticals.
The form of the external preparation is not particularly limited, and examples thereof include pastes, creams, gels, ointments, cosmetics, milky lotions, body lotions, powders, haptics, granules, and solid bath preparations.
The blending amount of the epidermis normalizing agent in such an external preparation is not particularly limited and can be used at any concentration, but the total blending amount of the plant extract according to the present invention is 0.00001 to 0.00001 in terms of dry matter. It is 10% by mass, more preferably 0.00002 to 5% by mass, and particularly preferably 0.0001 to 5% by mass.

In addition to the skin normalizing agent, the external preparation of the present invention includes functional ingredients known for external preparation as long as the effects of the present invention are not impaired, such as moisturizers, emollients, blood circulation promoters, and cell activators. , Antioxidants, anti-inflammatory agents, antibacterial agents, peroxide inhibitors, etc. can be blended.
As known functional ingredients, more specifically, moisturizers such as glycerin, urea, amino acids, hyaluronic acids; emollients such as squalane, macadamia nut oil, olive oil, jojoba oil, silicon oil; vitamin Es, peppermint tincture, etc. Blood circulation promoters; cell activators such as nucleic acids, antioxidants such as dibutylhydroxytoluene (BHT), dibutylhydroxyanisole (BHA), tocopherol acetate; anti-inflammatory agents such as glycyrrhizin, allantin; hinokithiol, benzalkonium chloride, Various functional ingredients such as antibacterial agents such as chlorhexidine salt and parahydroxybenzoic acid ester; and peroxide inhibitors such as superoxide dismutase (SOD) can be blended, and ginkgo extract, placenta extract, and lactic acid bacteria culture extract can be blended. Various extracts derived from plants, animals, and microorganisms such as these can be added and used without limitation.

In addition, the external preparation containing the epidermis normalizing agent of the present invention contains base components such as surfactants and oils and fats, and if necessary, for the purpose of forming the dosage form, as long as the effects of the present invention are not impaired. Various additives such as thickeners, preservatives, isotonic agents, antioxidants, ultraviolet absorbers, chelating agents, fragrances, and colorants can be used in combination.

The surfactants that can be used for the formulation of external preparations are not particularly limited, and for example, alkylene oxide adducts of higher alcohols, alkylene oxide adducts of higher alkylamines, alkylene oxide adducts of higher fatty acids, and higher fatty acid amides. Nonionic surfactants such as alkylene oxide adducts, fatty acid esters of polyhydric alcohols, alkylene oxide adducts of hardened castor oil, alkylene oxide adducts such as polyethylene glycol sorbitan alkyl esters and sterols; , Alpha-olefin sulfonate, polyoxyalkylene alkyl ether, anionic surfactants such as sodium sulfate; cationic surfactants such as alkylpyridinium chloride, distearyldimeryl ammonium chloride; sodium aminopropionate, alkylpolyaminoethylglycine And the like, an amphoteric surfactant and the like can be used. One type of these surfactants can be used, and two or more types can be used.

There are no particular restrictions on the base material components that can be used for molding external preparations, and known oils and fats such as olive oil, camellia oil, avocado oil, macadamia nut oil, apricot oil, jojoba oil, squalane, squalane, and horse oil. Can be used.

The thickener that can be used as an external preparation is not particularly limited, and for example, polyvinyl alcohol, polyvinylacrylamide, polyethylene glycol, and various derivatives thereof; celluloses such as hydroxyalkyl cellulose and derivatives thereof; dextran, gelatin, arabic gum. , Gum such as tragant gum; water-soluble polymers such as carboxyvinyl polymer can be used.

The preservative is not particularly limited, and for example, parahydroxybenzoic acid ester, paraoxybenzoate and its derivatives, phenoxyethanol, hinokitiol, benzalkonium chloride, chlorhexidine salt and the like can be used.

The tonicity agent is not particularly limited, and for example, inorganic salts such as sodium chloride, potassium chloride, and calcium chloride can be used.

The ultraviolet absorber is not particularly limited, and for example, para-aminobenzoic acid, a benzophenone-based ultraviolet absorber, a benzotriazole-based ultraviolet absorber, and the like can be used.

The chelating agent is not particularly limited, and for example, ethylenediaminetetraacetic acid, phytic acid, citric acid, and water-soluble salts thereof can be used.

Hereinafter, the present invention will be further described with reference to Examples, but the present invention is not limited to these Examples.
1. 1. Production of plant extract In Examples and Comparative Examples, the plant extracts obtained in the production examples shown below were used.

Production Example 1 Sage extract 50 g of sage was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 2.8 g of sage extract.

Production Example 2 Kagosou Extract 50 g of Kagosou was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 3.1 g of Kagosou extract.

Production Example 3 Marjoram Extract 50 g of marjoram was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 3.4 g of marjoram extract.

Production Example 4 Schizonepeta extract 50 g of schizonepeta was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 4.1 g of Keigai extract.

Production Example 5 Chinese mesona extract 50 g of Chinese mesona was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 3.5 g of Chinese mesona extract.

Production Example 6 Peppermint extract 50 g of peppermint was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 2.8 g of peppermint extract.

Production Example 7 Spearmint extract 50 g of spearmint was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 4.6 g of spearmint extract.

Production Example 8 Chimpi Extract 50 g of chimpi was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 4.2 g of chimpi extract.

Production Example 9 Rosemary extract 50 g of rosemary was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 4.3 g of rosemary extract.

Production Example 10 Prunes Extract 50 g of prunes were collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 2.3 g of prune extract.

Production Example 11 Eucalyptus extract 50 g of eucalyptus was collected and immersed in 375 ml of a 50 volume% ethanol aqueous solution at room temperature (about 25 ° C.). After 24 hours, this was filtered, and water and ethanol were distilled off under reduced pressure from the obtained extract and then freeze-dried to obtain 2.4 g of eucalyptus extract.

2. Evaluation of cornified envelope formation promoting effect (1) Sample preparation method 50% by volume ethanol for sage extract, kagosou extract, majorum extract, keigai extract, rensensou extract, peppermint extract, spearmint extract, chimpi extract, rosemary extract, and prune extract A 10 mg / mL, 2 mg / mL solution was prepared using mint, and then sterilized and filtered with a 0.22 μm membrane filter. These solutions were added so that the final ethanol concentration was 0.5% by volume with respect to the amount of the medium to prepare 100 μg / mL and 20 μg / mL extract-added media.
Similarly, eucalyptus extract was used for comparison.

(2) Cell culture As cells, human immortalized epidermal normal keratinocytes (PHK16-0b, National Institutes of Biomedical Innovation) were used. Subconfluent PHK16-0b cells were detached by trypsin treatment, 300,000 cells were seeded in each well of a 24-well plate, and the cells were inoculated in MCDB153 medium (manufactured by Sigma) for 24 hours at 37 ° C. and 5% CO ₂ conditions. It was cultured. After that, MCDB to which sage extract, Kagosou extract, Majorum extract, Keigai extract, Rensensou extract, peppermint extract, spearmint extract, chimpi extract, rosemary extract, and prune extract, which were adjusted to the concentrations shown in Tables 1 and 2, were appropriately added. The medium was added and the mixture was cultured for 24 hours. Instead of the sample, an aqueous ethanol solution having a final concentration of 0.5% by volume was used as a negative control.
After 24 hours, the medium was removed, 200 μL of ISOGENII (manufactured by Nippon Gene Co., Ltd.) was added to lyse the cells, and the cells were collected to extract RNA. The extraction method followed the standard method presented by Nippon Gene. The purified RNA concentration was measured using a spectrophotometer.

(3) Measurement of RNA expression level (3-1) Involucrin production promoting effect One Step SYBR PrimeScript RT-PCR KitII (manufactured by Takara Bio Inc.) is used to prepare a measurement sample according to the standard method presented by Takara Bio Inc. bottom. These samples are from Thermal Cycler Dice Real.
Real-time quantitative PCR was performed using Time System II (manufactured by Takara Bio Inc.) according to the standard method presented by Takara Bio Inc. For the mRNA expression level of the involucrin gene, the relative expression level was calculated using GAPDH as the housekeeping gene, and the involucrin mRNA level in the case of a negative control was shown as 100.

(3-2) Effect of promoting transglutaminase production The transglutaminase gene was measured instead of the involucrin gene described in the previous section, and the relative expression level was calculated.
The involucrin mRNA expression ratio is shown in Table 1, and the transglutaminase mRNA expression ratio is shown in Table 2.

The concentration (μg / mL) of the involucrin production promoting component in Table 1 represents the ratio to the total amount of the medium. As the results in Table 1 show, it was confirmed that when the epidermal normalizing agent of the present invention was used, an excellent production promoting effect was brought about for involucrin production in human immortalized epidermal normal keratinized cells.
The concentration (μg / mL) of the transglutaminase production promoting component in Table 2 represents the ratio to the total amount of the medium. As the results of the examples in Table 2 show, it was confirmed that when the epidermal normalizing agent of the present invention was used, it brought about an excellent production promoting effect on transglutaminase production in human immortalized epidermal normal keratinized cells. Was done.
That is, the epidermal normalizing agent of the present invention has both an involucrin production promoting effect and a transglutaminase promoting effect, and remarkably promotes cornified envelope formation in human immortalized epidermal normal keratinized cells.

3. 3. External preparations The following are examples of skin external preparations containing the plant extract of the present invention.
Examples 25 to 39 and Comparative Examples 33 to 46 (toner)
(component)
(Component A) (% by mass)
(1) PEG-40 hydrogenated castor oil 1.5
(2) 1,3-butylene glycol 5.0
(3) Dipropylene glycol 5.0
(4) Methylparaben 0.2
(Component B)
(5) Each plant extract 1 Amount listed in Tables 3 to 6 (6) Each plant extract 2 Amount listed in Tables 3 to 6 (7) Sodium hyaluronate solution (1%) 5.0
(8) Remaining amount of purified water
Total amount 100

(Manufacturing method)
1. 1. Components A (1) to (4) were mixed and dissolved at 70 ° C.
2. Ingredients B (5) to (8) were mixed and dissolved at room temperature.
3. 3. The component A was cooled to room temperature, and the component B was added little by little and mixed uniformly to obtain a lotion.

(evaluation)
The above-mentioned external preparations for skin (toner) were evaluated for a total of 112 people (28 groups x 2 prescriptions), two men and two men in their 30s and 50s who complained of dry skin. The lotion is appropriately used on the face twice a day (morning and night), with the lotion of Examples 25 to 39 or Comparative Examples 34 to 46 on one side of the face and the lotion of Comparative Example 33 on the other side. Was used, and a monitor test was conducted in which this was continued for one month. Evaluation was performed according to the following criteria in comparison with each comparative example. The results of the total score are shown in Tables 3-6.

During the evaluation period, the subjects of the monitor test were asked to use only the lotion of the present invention, but for other cosmetics, the ones normally used were used as they were. None of them complained of skin abnormalities during the period of use.
+3: Very moisturized than Comparative Example 33 +2: Much more moisturized than Comparative Example 33 +1: Slightly moisturized than Comparative Example 33 0: No difference -1: Comparative Example 33 was slightly moisturized -2: Comparative example 33 was considerably moisturized -3: Comparative example 33 was very moisturized

The external preparations of Examples 25 to 39 in which one or more selected from the group consisting of the cornified envelope formation promoter of the present invention and the chimpi extract, rosemary extract and kagosou extract were blended showed improvement in skin dryness. .. On the other hand, no change was observed in the external preparations of Comparative Examples 34 to 45. In addition, the external preparation of Comparative Example 46 was obtained by increasing the amount of the plant extract, but the improving effect on the dryness of the skin was inferior to that of Comparative Example 33, which was a negative control.

The epidermis normalizing agent of the present invention has an excellent epidermis normalizing effect, normalizes the skin epidermis barrier function, improves the water retention of the skin epidermis, and includes cosmetics for the purpose of preventing rough skin. It can be used for various external preparations.
In addition, the external preparation of the present invention has an excellent epidermis normalizing effect, can normalize the skin epidermis barrier function, improve the water retention of the skin epidermis, and prevent rough skin.

Claims (4)

At least one plant extract selected from the group consisting of sage extract, Kagosou extract, Majorum extract, Keigai extract, Rensensou extract, peppermint extract and spearmint extract, and
A cornified envelope formation promoter containing at least one plant extract selected from the group consisting of chimpi extract, rosemary extract and prune extract. A cornified envelope formation promoter containing at least one plant extract selected from the group consisting of sage extract, basket extract, and Chinese mesona extract, and a chimpi extract. A cornified envelope formation promoter containing at least one plant extract selected from the group consisting of sage extract, marjoram extract, and peppermint extract, and rosemary extract. A cornified envelope formation promoter containing at least one plant extract selected from the group consisting of sage extract, sage extract, and spearmint extract, and a prune extract.