JP7657627B2 - Liposome composition for cosmetics - Google Patents

Description

This disclosure relates to a liposome composition for cosmetics.

Liposomes are closed vesicles formed by a lipid bilayer membrane containing lipids such as lecithin, and have an aqueous phase (internal aqueous phase) within the space of the closed vesicle. In addition, liposomes usually exist in a dispersed state in an aqueous solution (external aqueous phase) outside the closed vesicle.

In recent years, liposomes containing medicinal or cosmetic ingredients in the internal aqueous phase have been considered for use in pharmaceutical compositions or cosmetics.

For example, Patent Document 1 proposes a liposome composition containing liposomes having an average particle size of 5 nm to 100 nm or less, which are contained in an internal aqueous phase containing a pharmaceutical ingredient.
Furthermore, Patent Document 2 proposes a liposome composition for cosmetics, which contains an oil or fat component in the internal aqueous phase and contains liposomes with an average particle size of 82 nm or less.
Furthermore, Patent Document 3 proposes a liposome composition for cosmetics, which contains a cosmetic ingredient in its internal aqueous phase and contains liposomes with an average particle size of 50 nm or less.
Furthermore, Patent Document 4 proposes a cosmetic composition that contains a disk-shaped bicellar structure formed by a lipid bilayer membrane, instead of a liposome.
Furthermore, Patent Document 5 proposes a cosmetic preparation containing vesicles in an external aqueous phase containing ethanol.

Patent No. 6251385 JP 2020-93992 A Patent No. 5064717 International Publication No. 2017/090740 JP 2016-56198 A

When liposomes encapsulating cosmetic ingredients are used in cosmetics, there is a demand for liposomes that can encapsulate a larger amount of cosmetic ingredients.
The present inventors have now discovered that there is room for improvement in the encapsulation rate of cosmetic ingredients in the liposomes contained in the compositions proposed in Patent Documents 1 to 3, the bicelle structure contained in the composition proposed in Patent Document 4, and the vesicles contained in the composition proposed in Patent Document 5.

From the standpoint of the encapsulation rate of the cosmetic ingredient, it is desirable that the liposomes contained in the liposome composition contain a large proportion of liposomes having a single lamellar structure in which the lipid bilayer is one layer (unilamellar vesicles).
The present inventors have further discovered that the compositions of Patent Documents 2, 3, and 5 have a small proportion of liposomes having a single lamellar structure, and the encapsulation rate of the cosmetic ingredient is insufficient.

This disclosure was made in consideration of the above circumstances, and the problem it aims to solve is to provide a liposome composition for cosmetics that contains liposomes with an improved encapsulation rate of a beauty ingredient.

<1> A liposome containing polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and a phytosterol other than the polyoxyethylene phytosterol, and having an average particle size of 100 nm to 350 nm;
The liposome composition for cosmetics comprises: an internal aqueous phase which contains water, ethanol and a cosmetic ingredient and is encapsulated in the liposome; and an external aqueous phase which contains water and ethanol and disperses the liposome.
<2> The liposome composition for cosmetics according to the above <1>, wherein the average number of moles of oxyethylene groups added in the polyoxyethylene phytosterol is 10 or more.
<3> The liposome composition for cosmetics according to <1> or <2>, wherein the content of the polyoxyethylene phytosterol in the liposome composition for cosmetics is 0.1% by mass or more.
<4> The liposome composition for cosmetics according to any one of <1> to <3>, wherein the content of the ethanol relative to the total mass of the liposome composition for cosmetics is 0.2% by mass to 30% by mass.
<5> The liposome composition for cosmetics according to any one of <1> to <4>, wherein the content of at least one of the cholesterol and the phytosterol other than polyoxyethylene phytosterol is 0.1% by mass to 0.5% by mass relative to the total mass of the liposome composition for cosmetics.
<6> The liposome composition for cosmetics according to any one of <1> to <5>, wherein the cosmetic ingredient is a water-soluble cosmetic ingredient.
<7> The liposome composition for cosmetics according to any one of <1> to <6>, wherein the cosmetic ingredient is at least one selected from the group consisting of caffeine, pyridoxine hydrochloride, and nicotinamide.
<8> The liposome composition for cosmetics according to any one of <1> to <7>, wherein a ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics is 70% or more.
<9> The liposome composition for cosmetics according to any one of <1> to <8>, which is a cosmetic.

According to the present disclosure, it is possible to provide a liposome composition for cosmetics containing liposomes with an improved encapsulation rate of a cosmetic ingredient.

1 shows a TEM (Transmission Electron Microscope) image of the liposome composition for cosmetics obtained in Example 1-2. 1 shows a TEM image of the liposome composition for cosmetics obtained in Example 3-1. 1 shows a TEM image of the liposome composition for cosmetics obtained in Example 3-2. 1 shows a TEM image of the liposome composition for cosmetics obtained in Comparative Example 1-2. 4 shows TEM images of the liposome compositions for cosmetics obtained in Comparative Examples 1-5.

The following describes in detail the form for implementing this disclosure. However, the present invention is not limited to the following embodiment. In the following embodiment, the components are not essential unless specifically stated otherwise. The same applies to numerical values and their ranges, and they do not limit the present disclosure.

In the present disclosure, the numerical ranges indicated using "to" include the numerical values before and after "to" as the minimum and maximum values, respectively.
In the numerical ranges described in the present disclosure in stages, the upper or lower limit value described in one numerical range may be replaced with the upper or lower limit value of another numerical range described in stages. In addition, in the numerical ranges described in the present disclosure, the upper or lower limit value of the numerical range may be replaced with a value shown in the examples.
In the present disclosure, each component may contain multiple types of corresponding compounds. When multiple substances corresponding to each component are present in the composition, the content of each component means the total content of the multiple substances present in the composition, unless otherwise specified.

In the present disclosure, a "liposome" is a closed endoplasmic reticulum formed by a lipid bilayer membrane.
A structure with a single lipid bilayer is called a single lamellar structure, and a structure with multiple lipid bilayers is called a multilamellar structure.
In this disclosure, "internal aqueous phase" refers to the solution present within closed endoplasmic reticulum.
In this disclosure, "external aqueous phase" refers to the solution in which the liposomes are dispersed.

In this disclosure, "cholesterol" is a general term for sterols contained in animals, and "phytosterol" is a general term for sterols contained in plants.

In the present disclosure, the term "beauty ingredient" refers to an ingredient that acts on the body, such as the skin, and means an active ingredient used in cosmetics and the like.
Moreover, the term "water-soluble" means that the solubility in 100 g of water at a liquid temperature of 22° C. and a pH of 7.0 is 0.1 g or more.

In the present disclosure, the term "average particle size" refers to the volume average particle size determined by dynamic light scattering.
Examples of dynamic light scattering particle size measuring instruments include Concentrated System Particle Size Analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.), Nanotrack UPA (manufactured by Nikkiso Co., Ltd.), and Dynamic Light Scattering Particle Size Distribution Measuring Instrument LB-550 (manufactured by Horiba, Ltd.).
The average particle size is measured by diluting the liposome composition for cosmetics with pure water to 10 times its mass, at room temperature (e.g., 25°C).
The "pure water" used herein is pure water obtained using an ultrapure water production apparatus manufactured by Merck Ltd.

In the present disclosure, "the ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics" is determined by observing the liposomes contained in the liposome composition for cosmetics in an ice-embedded state using a transmission electron microscope (TEM) and counting the total number of liposomes contained in the liposome composition for cosmetics and the total number of liposomes having a single lamellar structure.
More specifically, first, a TEM image of the liposome composition for cosmetic use at a magnification of 50,000 times is obtained using a transmission electron microscope.
The total number of liposomes present within a circle of radius 2 μm and the total number of liposomes having a single lamellar structure are counted at each of three arbitrary points on the TEM image to determine the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetic use."
In the present disclosure, the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics" is the average value of three points.
In the present disclosure, the encapsulation rate of a cosmetic ingredient in a liposome can be evaluated by the "average particle size of the liposomes" and the "ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics."

(Liposome composition for cosmetics)
The liposome composition for cosmetics according to the present disclosure comprises polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and a phytosterol other than the polyoxyethylene phytosterol, and comprises liposomes having an average particle size of 100 nm to 350 nm, water, ethanol, and a cosmetic ingredient, an inner aqueous phase that is encapsulated in the liposomes, and an outer aqueous phase that contains water and ethanol and disperses the liposomes.

The liposome composition for cosmetics disclosed herein can provide a liposome composition for cosmetics that contains liposomes with an excellent encapsulation rate of a cosmetic ingredient.

The reason why the above effect is achieved is presumed to be as follows, but is not limited to this.
In the liposome composition for cosmetics of the present disclosure, the liposomes contain polyoxyethylene phytosterol, which inhibits the lipid bilayer membranes from approaching each other, and polyoxyethylene phytosterol contributes to the formation of liposomes, so it is presumed that the average particle size of the liposomes formed and the proportion of liposomes having a single lamellar structure are increased, thereby improving the encapsulation rate of the cosmetic ingredient.

From the viewpoint of improving the encapsulation rate of a cosmetic ingredient, in the liposome composition for cosmetics, the ratio of the polyoxyethylene phytosterol content to the ethanol content (polyoxyethylene phytosterol content/ethanol content) is preferably 0.001 to 1, more preferably 0.002 to 0.98, and even more preferably 0.03 to 0.97, on a mass basis.
In addition, by setting the ratio of the polyoxyethylene phytosterol content to the ethanol content within the above numerical range, it is possible to prevent the liposomes from aggregating over time, which would increase the particle size of the liposomes and cause the suspension of the liposome composition for cosmetics.
Furthermore, by setting the ratio of the polyoxyethylene phytosterol content to the ethanol content within the above numerical range, it is possible to prevent aggregated liposomes from coalescing and becoming multilamellar, which would result in a decrease in the encapsulation rate of the beauty ingredient.
The above-mentioned ability to inhibit aggregation of liposomes is hereinafter also referred to as temporal stability.
The ratio of the polyoxyethylene phytosterol content to the ethanol content in the liposome composition for cosmetics is calculated by rounding off to the fourth decimal place.

In the liposome composition for cosmetics, the ratio of the polyoxyethylene phytosterol content to the cholesterol content (polyoxyethylene phytosterol content/cholesterol content) is preferably 2 or less, more preferably 1.7 or less, and even more preferably 1 or less, on a mass basis.
By setting the ratio of the polyoxyethylene phytosterol content to the cholesterol content in the cosmetic liposome composition to be 2 or less, on a mass basis, the proportion of liposomes having a single lamellar structure in the cosmetic liposome composition is increased, and the encapsulation rate of the cosmetic ingredient is further improved.
Furthermore, the lower limit of the ratio of the polyoxyethylene phytosterol content to the cholesterol content in the liposome composition for cosmetics is not particularly limited, but from the viewpoint of liposome formability, it is preferably 0.1 or more.
The ratio of the polyoxyethylene phytosterol content to the cholesterol content in the liposome composition for cosmetics is calculated by rounding off to one decimal place.

(Liposome)
In the liposome composition for cosmetics of the present disclosure, the liposomes have an average particle size of 100 nm to 350 nm.
When the average particle size of the liposomes is 100 nm or more, the encapsulation rate of the cosmetic ingredient is improved. Furthermore, when the average particle size of the liposomes is 100 nm or more, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics tends to be increased.
Liposomes with an average particle size of more than 350 nm have low stability over time, and tend to become multilamellar due to aggregation and coalescence over time, resulting in a decrease in the encapsulation rate of the cosmetic ingredient. By making the average particle size of the liposomes 350 nm or less, the multilamellar formation can be suppressed. In addition, when the average particle size of the liposomes is more than 350 nm, the turbidity of the liposome composition for cosmetics produced tends to increase. By making the average particle size of the liposomes 350 nm or less, the increase in turbidity of the liposome composition for cosmetics can be suppressed, and the appearance of the liposome composition for cosmetics tends to be excellent. Furthermore, by making the average particle size of the liposomes 350 nm or less, the permeability of the liposome composition for cosmetics into the skin, etc. tends to be improved.
From the viewpoint of improving the encapsulation rate of the cosmetic ingredient and stability over time, the average particle size of the liposomes is preferably 110 nm to 330 nm, more preferably 120 nm to 320 nm, and even more preferably 150 nm to 300 nm.

From the viewpoint of improving the encapsulation rate of the cosmetic ingredient, the liposomes are preferably liposomes having a single lamellar structure (also called unilamellar vesicles), and the ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition is preferably 50% or more, and more preferably 70% or more.
The liposome composition may contain a liposome having a multilamellar structure (also called a multilamellar vesicle).

The liposome contains a polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and a phytosterol other than the polyoxyethylene phytosterol.
More specifically, the lipid bilayer membrane constituting the liposome contains the above-mentioned components. Each component will be described below.

(Polyoxyethylene phytosterol)
Polyoxyethylene phytosterol is a phytosterol having an oxyethylene group represented by (CH ₂ CH ₂ —O) _n .
In this disclosure, phytosterols include hydrogenated phytosterols (phytostanols).
Specific examples of phytosterols include sitosterol, stigmasterol, fucosterol, spinasterol, brassicasterol, and hydrogenated products thereof.

From the viewpoint of improving the encapsulation rate of the cosmetic ingredient, the average number of moles of oxyethylene groups added in the polyoxyethylene phytosterol is preferably 5 or more, more preferably 10 or more, and even more preferably 20 or more.
Furthermore, by making the average number of moles of oxyethylene groups added to the polyoxyethylene phytosterol 5 or more, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the encapsulation rate of the cosmetic ingredient in the liposomes can be further improved. Note that the catalog value can be referred to for the average number of moles of oxyethylene groups added to the polyoxyethylene phytosterol.

Commercially available polyoxyethylene phytosterols include Nikkol (registered trademark) BPS-5 (average number of moles of oxyethylene groups added: 5), Nikkol (registered trademark) BPS-10 (average number of moles of oxyethylene groups added: 10), Nikkol (registered trademark) BPS-20 (average number of moles of oxyethylene groups added: 20), Nikkol (registered trademark) BPS-30 (average number of moles of oxyethylene groups added: 30), Nikkol (registered trademark) BPSH-25 (average number of moles of oxyethylene groups added: 25), all manufactured by Nikko Chemicals Co., Ltd. Ethylene oxide adduct of hydrogenated phytosterol), EMALEX (registered trademark) PS-5 (average number of moles of oxyethylene groups added: 5), EMALEX (registered trademark) PS-10 (average number of moles of oxyethylene groups added: 10), EMALEX (registered trademark) PS-20 (average number of moles of oxyethylene groups added: 20), EMALEX (registered trademark) PS-30 (average number of moles of oxyethylene groups added: 30), and EMALEX (registered trademark) PS-25 (average number of moles of oxyethylene groups added: 25), all manufactured by Nippon Emulsion Co., Ltd.

The content of polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and even more preferably 0.23% by mass or more.
By ensuring that the polyoxyethylene phytosterol content is 0.05% by mass or more, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the encapsulation rate of cosmetic ingredients in the liposomes can be further improved.
The content of polyoxyethylene phytosterol in the total mass of the liposome composition for cosmetics is preferably 1% by mass or less, and more preferably 0.70% by mass or less. By making the content of polyoxyethylene phytosterol 1% by mass or less, it is possible to suppress the formation of disk particles or micelles instead of liposomes.

(lecithin)
Examples of lecithin include natural lecithin obtained from plants such as soybean, rapeseed, sunflower, safflower, peanut, cottonseed, corn, rice, barley, and egg yolk, as well as derivatives of these (hereinafter also referred to as lecithin derivatives).
Examples of lecithin derivatives include hydrogenated lecithin, and compounds in which polyethylene glycol, aminoglycans, etc. are introduced into the phospholipids in lecithin.
Among the above, hydrogenated lecithin is particularly preferred from the viewpoint of oxidation stability.

From the viewpoint of the stability over time of the liposome composition for cosmetics, the content of phosphatidylcholine relative to the total mass of lecithin (hereinafter also referred to as PC content) is preferably 80% by mass or more, more preferably 85% by mass or more, and even more preferably 90% by mass or more.
The PC content of the phospholipid composition is measured by TLC-FID method or HPLC method.

Commercially available lecithin includes COATSOME NC-21 (PC content 90% or more by mass) manufactured by NOF Corporation, and Nikkol (registered trademark) Lecithinol S-10E (PC content 75% to 85% or more by mass) manufactured by Nikko Chemicals Co., Ltd.

From the viewpoint of lipid bilayer formation and stability over time, the content of lecithin relative to the total mass of the liposome composition for cosmetics is preferably 0.3% by mass to 10% by mass, more preferably 0.4% by mass to 5% by mass, and even more preferably 0.5% by mass to 3% by mass.

(Phytosterols other than cholesterol and polyoxyethylene phytosterols)
In this disclosure, cholesterol includes cholestanol and cholesterol derivatives.
Examples of cholesterol include cholesterol, dihydrocholesterol, dehydrocholesterol, cholesteryl oleate, cholesteryl isostearate, cholesteryl hydroxystearate, and polyoxyethylene cholesteryl ether.
Moreover, phytosterols other than polyoxyethylene phytosterols refer to phytosterols that do not have a polyoxyethylene group.
Moreover, from the viewpoint of the encapsulation rate of the cosmetic ingredient, the liposome composition for cosmetics according to the present disclosure preferably contains at least cholesterol.

The content of at least one of cholesterol and phytosterol other than polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is preferably 0.05% by mass to 0.7% by mass, more preferably 0.1% by mass to 0.5% by mass, and even more preferably 0.2% by mass to 0.4% by mass.
By setting the content of at least one of cholesterol and phytosterol other than polyoxyethylene phytosterol within the above numerical range, the proportion of liposomes having a single lamellar structure in the liposome composition for cosmetics can be increased, and the encapsulation rate of the cosmetic ingredient in the liposomes can be further improved.
In addition, in the present disclosure, "the content of at least one of cholesterol and phytosterols other than polyoxyethylene phytosterols relative to the total mass of the liposome composition for cosmetics" means the content of cholesterol when the liposome composition for cosmetics contains only cholesterol, means the content of cholesterol when the liposome composition for cosmetics contains only phytosterol, and means the sum of the contents of cholesterol and phytosterol when the liposome composition for cosmetics contains both cholesterol and phytosterol.

(Domestic and foreign waters)
The liposome composition for cosmetics according to the present disclosure comprises an internal aqueous phase and an external aqueous phase.
The internal aqueous phase contains at least water, ethanol, and a cosmetic ingredient, and the external aqueous phase contains at least water and ethanol. By containing ethanol in the internal aqueous phase and the external aqueous phase, it is possible to improve the formability and temporal stability of liposomes.

(water)
Examples of water include ion-exchanged water, pure water, purified water, and tap water. Among these, purified water is preferred in view of its applicability to cosmetics.

The content of water relative to the total mass of the liposome composition for cosmetics is not particularly limited, but is preferably 1% by mass to 99% by mass, more preferably 10% by mass to 95% by mass, and even more preferably 20% by mass to 90% by mass.
In the present disclosure, the "water content relative to the total mass of the liposome composition for cosmetics" means the sum of the water content contained in the internal aqueous phase and the water content contained in the external aqueous phase relative to the total mass of the liposome composition for cosmetics.

(ethanol)
From the viewpoint of stability over time, the content of ethanol relative to the total mass of the liposome composition for cosmetics is preferably 0.2% by mass to 30% by mass.
Furthermore, the content of ethanol relative to the total mass of the liposome composition for cosmetics may be 3% by mass or more, 9% by mass or more, or 15% by mass or more.
Furthermore, the content of ethanol relative to the total mass of the liposome composition for cosmetics may be 27% by mass or less, 25% by mass or less, 23% by mass or less, or 10% by mass or less.
In the present disclosure, the "ethanol content relative to the total mass of the liposome composition for cosmetics" means the sum of the ethanol content contained in the inner aqueous phase and the ethanol content contained in the outer aqueous phase relative to the total mass of the liposome composition for cosmetics.
Incidentally, ethanol includes absolute ethanol.

(Beauty ingredients)
Cosmetic ingredients include ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpantothenyl ethyl ester, disodium adenosine triphosphate, disodium adenosine monophosphate, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin-β-glycyrrhetinic acid, albumin, inositol, erythritol, and gluconate hydrochloride. samine, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, soluble elastin, taurine, sodium palmitoyl methyl taurine, sodium myristoyl methyl taurine, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcinol, levulinic acid, glycine, arginine, lysine solution, lauroyl lysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, retinol palmitate, alginic acid, gamma-undecalactone, perilla oil, estradiol, estrone, ergocalciferol, Examples of such ingredients include β-carotene, glucosamine, cholecalciferol, tocopherol acetate, retinol acetate, salicylic acid, shikonin, ascorbyl dipalmitate, pyridoxine dipalmitate, natural vitamin E, α-tocopherol, horse oil, γ-nonalactone, bisabolol, vitamin A oil, hinokitiol, fumaric acid, powdered vitamin A, tocopherol linoleate, δ-tocopherol, tocopherol linoleate, isoleucine, phenylalanine, valine, leucine, ascorbyl tetra-2-hexyldecanoate, etc. Caffeine includes anhydrous caffeine.
From the viewpoint of solubility, the cosmetic ingredient contained in the internal aqueous phase is preferably a water-soluble cosmetic ingredient, and among the above, ascorbic acid, ascorbic acid stearate, sodium ascorbate, disodium ascorbate sulfate, aspartic acid, aspartame, acetylglucosamine, acetylglutamic acid, acetylcysteine, acetylpantothenyl ethyl ester, disodium adenosine triphosphate, disodium adenosine monophosphate, ε-aminocaproic acid, γ-aminobutyric acid, allantoin, allantoin β-glycyrrhetinic acid, albumin, inositol, erythritol, glucosamine hydrochloride, pyridoxine hydrochloride, oxyproline, orotic acid, hydrolyzed elastin, caffeine hydrate, sodium chondroitin sulfate, cyanocobalamin, water, Soluble elastin, taurine, sodium palmitoyl methyl taurine, sodium myristoyl methyl taurine, thiamine hydrochloride, theanine, deoxyribonucleic acid, ascorbyl palmitate, pantothenyl alcohol, sodium pantothenate, calcium pantothenate, pyridoxine, phytic acid, placenta extract, flavin adenine dinucleotide disodium dihydrate, anhydrous caffeine, riboflavin, riboflavin butyrate, riboflavin phosphate, resorcinol, levulinic acid, glycine, arginine, lysine solution, lauroyl lysine, aspartic acid, palmitoyl aspartic acid, cysteine, methionine, glutamic acid, threonine, serine, tyrosine, histidine, proline, glutathione, salicylic acid, nicotinamide, and the like are preferred.
Furthermore, from the viewpoints of anti-aging, anti-wrinkle, anti-blemish, anti-firmness, and the like, among the above, it is more preferable that the internal aqueous phase contains at least one selected from caffeine, pyridoxine hydrochloride, and nicotinamide.
The cosmetic ingredient may also be contained in the external aqueous phase.

The content of the cosmetic ingredient relative to the total mass of the liposome composition for cosmetics is preferably 0.3% by mass to 5% by mass, and more preferably 0.5% by mass to 3% by mass.

The inner aqueous phase and the outer aqueous phase may contain other components such as physiological saline, sugar, a pH adjuster, and a preservative. Examples of sugar include glucose, fructose, lactose, sucrose, trehalose, lactulose, and maltitol. Examples of pH adjusters include sodium hydrogen phosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate anhydrous, sodium hydroxide (caustic soda), citric acid, acetic acid, and triethanolamine. Examples of preservatives include methyl paraoxybenzoate.

The pH of the inner aqueous phase and the outer aqueous phase is not particularly limited, but is preferably 5 to 9, and more preferably 7 to 8. The pH of the inner aqueous phase and the outer aqueous phase can be adjusted by using the pH adjuster described above.

A method for producing the liposome composition for cosmetics according to the present disclosure will be described below, but is not limited thereto.
The liposome composition for cosmetics can be produced by mixing an oil phase composition and an aqueous phase composition, stirring and emulsifying the mixture to form liposomes.

By mixing, stirring, and emulsifying the oil phase composition and the aqueous phase composition, the oil phase composition and the aqueous phase composition are emulsified into an O/W type (oil-in-water type) emulsion, and a liposome composition for cosmetics containing liposomes, an inner aqueous phase, and an outer aqueous phase is produced.

Ultrasonic or mechanical shearing force is used as a stirring method. In addition, to make the particle size uniform, an extruder process or a microfluidizer process can be performed by passing the mixture through a filter with a certain pore size. By performing an extruder process or the like, a multivesicular liposome containing multiple univesicular liposomes can be separated into multiple univesicular liposomes.

The liquid temperature of the mixture can be adjusted as appropriate, but it is preferable to set it at a temperature higher than the phase transition temperature of the lecithin contained in the mixture, for example, at 35°C to 70°C.

In producing a liposome composition for cosmetics, the organic solvent and water may be evaporated from an aqueous solution containing liposomes.
The evaporation mentioned above includes both intentional evaporation of a part or all of the organic solvent and water, and natural evaporation of a part or all of the organic solvent and water during the stirring and emulsification process.

When the organic solvent and water are intentionally evaporated, the evaporation method is not particularly limited, but examples include a method of heating the organic solvent and water, a method of leaving the liposome composition for cosmetic use stationary, a method of stirring the liposome composition for cosmetic use, a method of vacuum degassing, etc.

The method for encapsulating cosmetic ingredients in liposomes is not particularly limited, and can be achieved by using an aqueous phase composition in which the cosmetic ingredients are dissolved during emulsification.

The obtained liposome composition for cosmetics may be subjected to dialysis, filtration, extrusion processing, or the like, which can make the average particle size of the liposomes contained therein uniform. The extrusion processing is a method in which the liposome composition for cosmetics is passed through a filter having fine holes, thereby applying a physical shearing force to the composition, thereby atomizing the composition. When passing the liposome composition for cosmetics, the liposome composition for cosmetics and the filter are kept at a temperature equal to or higher than the phase transition temperature of lecithin, thereby enabling rapid atomization.

(Application)
In one embodiment, the liposome composition for cosmetics of the present disclosure is a cosmetic. The form of the cosmetic is not particularly limited, and may be liquid, jelly, gel, cream, solid such as a stick, or semi-solid. Examples of the cosmetic include skin care cosmetics (skin lotion, emulsion, serum, sunscreen (sunscreen agent), etc.), body cosmetics (body lotion, etc.), scalp cosmetics, packs, etc., but are not limited thereto.

All publications, patent applications, and technical standards described in this specification are incorporated by reference into this specification to the same extent as if each individual publication, patent application, and technical standard was specifically and individually indicated to be incorporated by reference.

The above embodiment will be explained in detail below using examples, but the above embodiment is not limited to these examples.

<Example 1-1>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol A (average number of moles of oxyethylene groups added: 20), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes, to obtain an oil phase composition A.

(Preparation of Aqueous Phase Composition)
Anhydrous caffeine (a cosmetic ingredient), nicotinamide, disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water were mixed and dissolved by heating at 60° C. for 10 minutes, to obtain an aqueous phase composition A.

(Production of liposome composition for cosmetics)
The aqueous phase composition A obtained above was stirred with a homomixer while being kept at 60° C. (3,400 rpm (revolutions per minute)), and then the oil phase composition A was added to the aqueous phase composition A.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35° C. or lower, and then filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetic use.
The content of each component in the liposome composition for cosmetic preparations is as shown in Table 1.
In the liposome composition for cosmetics obtained in Example 1-1, the ratio of the polyoxyethylene phytosterol content to the ethanol content (polyoxyethylene phytosterol content/ethanol content) was 0.002 on a mass basis.
In addition, in the liposome composition for cosmetics obtained in Example 1-1, the ratio of the polyoxyethylene phytosterol content to the cholesterol content (polyoxyethylene phytosterol content/cholesterol content) was 0.2 on a mass basis.
In Table 1, the ratio of polyoxyethylene phytosterol content to ethanol content is indicated as "P/E", and the ratio of polyoxyethylene phytosterol content to cholesterol content is indicated as "P/C".
The content of each component, P/E and P/C for the following examples are also shown in Tables 1 to 3.

<Examples 1-2 to 1-4 and Comparative Examples 1-1 to 1-5>
A liposome composition for cosmetic preparations was produced in the same manner as in Example 1-1, except that the composition of the liposome composition for cosmetic preparations was changed as shown in Table 1.
In Comparative Example 1-1, liposome formation was not possible, and therefore the subsequent evaluation results in Table 1 are marked with "-".

<Example 1-5>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol A (average number of moles of oxyethylene groups added: 20), cholesterol, oleic acid, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes, to obtain an oil phase composition B.

(Preparation of Aqueous Phase Composition)
Pyridoxine hydrochloride (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium hydroxide solution (caustic soda), and purified water were mixed and dissolved by heating at 60°C for 10 minutes, to obtain aqueous phase composition B.

(Preparation of liposome composition)
The aqueous phase composition B obtained above was stirred (3,000 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition B was added to the aqueous phase composition B.
After the addition, stirring was continued for 45 minutes at 3000 rpm. After stirring, the mixture was cooled to 35° C. or lower and filtered through a 230 mesh filter cloth, and then the ethanol was evaporated using an evaporator to obtain a liposome composition for cosmetics.
The content of each component in Table 1 is the content in the liposome composition for cosmetics after evaporation of the ethanol.

<Examples 1-6 to 1-7>
Except for changing the composition of the liposome composition for cosmetic preparations as shown in Table 1, liposome compositions for cosmetic preparations were produced in the same manner as in Example 1-5.

<Examples 2-1 to 2-3>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol B (average number of moles of oxyethylene groups added: 30), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes, to obtain an oil phase composition C1.

(Preparation of Aqueous Phase Composition)
Anhydrous caffeine (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water were mixed and dissolved by heating at 60°C for 10 minutes, thereby obtaining aqueous phase composition C.

(Production of liposome composition for cosmetics)
The aqueous phase composition C obtained above was stirred (3,400 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition C1 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35° C. or lower, and then filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetic use.

<Example 2-4>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol C (average number of moles of oxyethylene groups added: 10), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes, to obtain an oil phase composition C2.

(Preparation of Aqueous Phase Composition)
Anhydrous caffeine (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water were mixed and dissolved by heating at 60°C for 10 minutes, thereby obtaining aqueous phase composition C.

(Production of liposome composition for cosmetics)
The aqueous phase composition C obtained above was stirred (3,400 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition C2 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35° C. or lower, and then filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetic use.

<Example 2-5>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% or more), polyoxyethylene phytosterol D (average number of moles of oxyethylene groups added: 5), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60°C for 10 minutes, to obtain an oil phase composition C3.

(Preparation of Aqueous Phase Composition)
Anhydrous caffeine (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water were mixed and dissolved by heating at 60°C for 10 minutes, thereby obtaining aqueous phase composition C.

(Production of liposome composition for cosmetics)
The aqueous phase composition C obtained above was stirred (3,400 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition C3 was added to the aqueous phase composition C.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, the mixture was cooled to 35° C. or lower, and then filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetic use.

<Example 3-1>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% by mass or more), polyoxyethylene phytosterol A (average number of moles of oxyethylene groups added: 20), cholesterol, absolute ethanol and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60° C. for 10 minutes, to obtain an oil phase composition D.

(Preparation of Aqueous Phase Composition)
Pyridoxine hydrochloride (beauty ingredient), anhydrous caffeine (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium dihydrogen phosphate and purified water were mixed and dissolved by heating at 60°C for 10 minutes, to obtain aqueous phase composition D-1.
Also, methyl paraoxybenzoate and purified water were mixed and dissolved by heating at 60° C. for 10 minutes to obtain an aqueous phase composition D-2.

(Production of liposome composition for cosmetics)
The aqueous phase composition D-1 obtained above was stirred (3400 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition D was added to the aqueous phase composition D-1.
After the addition, stirring was continued for 45 minutes at 3400 rpm. After stirring, aqueous phase composition D-2 was added, and the mixture was stirred uniformly for 5 minutes and cooled to 35° C. or lower. After cooling, the mixture was filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetics.

<Example 3-2>
(Preparation of Oil Phase Composition)
Hydrogenated soybean phospholipid (hydrogenated lecithin, PC content of 90% by mass or more), polyoxyethylene phytosterol A (average number of moles of oxyethylene groups added: 20), cholesterol, oleic acid, absolute ethanol, and ascorbyl tetra-2-hexyldecanoate were mixed and dissolved by heating at 60° C. for 10 minutes, to obtain an oil phase composition E.

(Preparation of Aqueous Phase Composition)
Pyridoxine hydrochloride (beauty ingredient), nicotinamide (beauty ingredient), disodium hydrogen phosphate anhydrous, sodium hydroxide solution (caustic soda), and purified water were mixed and dissolved by heating at 60°C for 10 minutes to obtain aqueous phase composition E-1.
Also, methyl paraoxybenzoate and purified water were mixed and dissolved by heating at 60° C. for 10 minutes to obtain an aqueous phase composition E-2.

(Production of liposome composition for cosmetics)
The aqueous phase composition E-1 obtained above was stirred (3000 rpm) with a homomixer while being kept at 60° C., and then the oil phase composition E was added to the aqueous phase composition E-1.
After the addition, stirring was continued for 45 minutes at 3000 rpm. After stirring, aqueous phase composition E-2 was added, and the mixture was stirred uniformly for 5 minutes and cooled to 35° C. or lower. After cooling, the mixture was filtered through a 230 mesh filter cloth to obtain a liposome composition for cosmetics.

<<Encapsulation rate evaluation-1 (proportion of liposomes with single lamellar structure)>>
The liposomes contained in the liposome compositions for cosmetics produced in the Examples and Comparative Examples were embedded in ice and TEM images were taken at a magnification of 50,000 times using a transmission electron microscope.

The total number of liposomes present within a circle of radius 2 μm and the total number of liposomes having a single lamellar structure were counted at three arbitrary points on the TEM image, and the average ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetic use was confirmed and evaluated based on the following evaluation criteria. The evaluation results are shown in Tables 1 to 3.
TEM images of Example 1-2, Example 3-1, Example 3-2, Comparative Example 1-2, and Comparative Example 1-5 are shown in Figures 1 to 5. The total number of liposomes and the total number of liposomes having a single lamellar structure are indicated by circles shown in Figures 1 to 5, which represent an arbitrary circle where the total number of liposomes and the total number of liposomes having a single lamellar structure were counted.
(Evaluation Criteria)
A: The proportion of liposomes having a single lamellar structure was 70% or more.
B: The ratio of liposomes having a single lamellar structure was 50% or more and less than 70%.
C: The ratio of liposomes having a single lamellar structure was 30% or more and less than 50%.
D: The proportion of liposomes having a single lamellar structure was less than 30%.

<<Encapsulation rate evaluation-2 (average particle size of liposomes)>>
The liposome compositions for cosmetics produced in the Examples and Comparative Examples were diluted 10 times by mass with pure water, and the volume average particle size of the liposomes contained therein was measured by dynamic light scattering using a concentrated particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd.). The volume average particle size was measured within 20 to 24 hours after the production of the liposome compositions for cosmetics. The temperature of the measurement environment was 25°C. The measurement results are shown in Tables 1 to 3.

<<Evaluation of stability over time>>
The liposome compositions for cosmetics produced in Examples 1-5 to 1-7, 2-1 to 2-5, 3-1 to 3-2, and Comparative Example 1-4 were allowed to stand in an environment of 50° C. for one week.
The liposome composition for cosmetics after standing was diluted 10 times by mass with pure water, and the volume average particle size of the liposomes contained in the liposome composition for cosmetics was measured by the method described above.
The ratio of the volume average particle diameter of the liposomes contained in the liposome composition for cosmetic preparations after standing to the volume average particle diameter of the liposomes contained in the liposome composition for cosmetic preparations immediately after production (volume average particle diameter of the liposomes contained in the liposome composition for cosmetic preparations after standing/volume average particle diameter of the liposomes contained in the liposome composition for cosmetic preparations immediately after production) was determined, and the stability over time of the liposome composition for cosmetic preparations was evaluated based on the following evaluation criteria. The evaluation results are shown in Tables 1 to 3.
In addition, in Comparative Examples 1-2 and 1-3, the proportion of liposomes having a single lamellar structure was small and the encapsulation rate of the cosmetic ingredient was insufficient, so evaluation of stability over time was not performed, and the evaluation results are indicated as "-" in Table 1.
In addition, in Comparative Example 1-5, since the evaluation of the encapsulation rate was insufficient, the evaluation of the stability over time was not performed, and the evaluation result in Table 1 is marked with "-".
(Evaluation Criteria)
A: The volume average particle size ratio was less than 1.1.
B: The volume average particle size ratio was 1.1 or more and less than 1.4.
C: The volume average particle size ratio was 1.4 or more.

The results in Table 1 show that the liposome compositions obtained in Examples 1-1 to 1-7 have a higher proportion of liposomes having a single lamellar structure and are superior in encapsulation rate of cosmetic ingredients compared to the liposome compositions obtained in Comparative Examples 1-2 to 1-3 which do not contain at least one of polyoxyethylene phytosterol and ethanol.
Furthermore, the results in Table 1 show that the liposome compositions obtained in Examples 1-1 to 1-7 have a higher proportion of liposomes having a single lamellar structure and are superior in encapsulation rate of cosmetic ingredients compared to the liposome composition obtained in Comparative Example 1-5, which does not contain at least one of cholesterol and phytosterols other than polyoxyethylene phytosterol and contains liposomes with an average particle size of less than 100 nm.
Furthermore, it can be seen that the liposomes contained in the liposome compositions obtained in Examples 1-1 to 1-7 have a large average particle size and are excellent in the encapsulation rate of the cosmetic ingredient.
In Comparative Examples 1-2 to 1-3, the content of the cosmetic ingredient is the same as in Examples 1-1 to 1-7, but because the proportion of liposomes having a single lamellar structure contained in the obtained liposome composition is small, most of the cosmetic ingredient is contained in the external aqueous phase rather than the internal aqueous phase.
It can be seen that the liposome compositions obtained in Examples 1-1 to 1-7 have superior stability over time compared to the liposome composition obtained in Comparative Example 1-4, which does not contain polyoxyethylene phytosterol and contains liposomes with an average particle size of more than 350 nm.
Incidentally, it is presumed that the liposome forming property was low in Comparative Example 1-1 because ethanol was not contained.

The results in Tables 2 and 3 show that the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 have a high proportion of liposomes having a single lamellar structure and are excellent in encapsulation rate of cosmetic ingredients.
Furthermore, it can be seen that the liposomes contained in the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 have a large average particle size and are excellent in encapsulation rate of the cosmetic ingredient.
Furthermore, it is understood that the liposome compositions obtained in Examples 2-1 to 2-5 and Examples 3-1 to 3-2 have excellent stability over time.

Figures 1 to 5 show that the liposome composition obtained in the examples has a higher proportion of liposomes with a single lamellar structure and is superior in encapsulation rate of cosmetic ingredients compared to the liposome composition obtained in the comparative examples. Figure 5 also shows that a large amount of disk particles, rather than liposomes, is included.

Below, we disclose formulation examples in which the liposome composition for cosmetics disclosed herein is used as a cosmetic.

Example 4-1: Cream
A cream having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-1: 2.0% by mass
Hydrogenated polyisobutene 10.0% by mass
Dipotassium glycyrrhizinate 1.0% by mass
Vaseline 8.0% by mass
Lauroyl glutamic acid di(phytosteryl/octyldodecyl) 12.0% by mass
Dimer dilinoleic acid (phytosteryl/isostearyl/cetyl/stearyl/behenyl) 4.0% by mass
Dimethicone 6.0% by mass
1,2-pentanediol 3.0% by mass
Dipropylene glycol 7.0% by mass
Concentrated glycerin 9.0% by mass
Polyethylene glycol 6000 (weight average molecular weight: 6000) 1.0% by mass
Sodium hyaluronate 0.5% by mass
Behenyl alcohol 2.0% by mass
Polyglyceryl monostearate 3.0% by mass
Tocopherol 0.5% by mass
Astaxanthin 0.2% by mass
Water-soluble collagen 1.0% by mass
Hydrolyzed collagen solution (fish-derived) 1.0% by mass
N-acetyl-L-hydroxyproline 1.0% by mass
Citric acid 0.7% by mass
Sodium citrate (appropriate amount) Phenoxyethanol (0.3% by weight)
- Remainder: Purified water

<Example 4-2: Cream>
A cream having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-2: 2.0% by mass
Hydrogenated polyisobutene 10.0% by mass
Dipotassium glycyrrhizinate 1.0% by mass
Vaseline 8.0% by mass
Lauroyl glutamic acid di(phytosteryl/octyldodecyl) 12.0% by mass
Dimer dilinoleic acid (phytosteryl/isostearyl/cetyl/stearyl/behenyl) 4.0% by mass
Dimethicone 6.0% by mass
1,2-pentanediol 3.0% by mass
Dipropylene glycol 7.0% by mass
Concentrated glycerin 9.0% by mass
Polyethylene glycol 6000 (weight average molecular weight: 6000) 1.0% by mass
Sodium hyaluronate 0.5% by mass
Behenyl alcohol 2.0% by mass
Polyglyceryl monostearate 3.0% by mass
Tocopherol 0.5% by mass
Astaxanthin 0.2% by mass
Water-soluble collagen 1.0% by mass
Hydrolyzed collagen solution (fish-derived) 1.0% by mass
N-acetyl-L-hydroxyproline 1.0% by mass
Citric acid 0.7% by mass
Sodium citrate (appropriate amount) Phenoxyethanol (0.3% by weight)
- Remainder: Purified water

The creams obtained in Examples 4-1 and 4-2 had good properties in terms of usability, fragrance, etc., and it was found that the liposome composition for cosmetics disclosed herein can be suitably used for preparing creams.

Example 5-1: Lotion
A lotion having the following composition was prepared in a conventional manner (total amount 100% by mass).
(composition)
Liposome composition of Example 3-1: 2.0% by mass
Arbutin 2.0% by mass
Dipotassium glycyrrhizinate 1.0% by mass
Dipropylene glycol 4.0% by mass
Polyoxyethylene methyl glucoside 1.0% by mass
1,3-butylene glycol 4.0% by mass
Polyethylene glycol 1.0% by mass
Ethanol 2.0% by mass
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Phenoxyethanol 0.3% by mass
Glycerin mono-2-ethylhexyl ether 0.2% by mass
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
N-acetyl-L-hydroxyproline 1.0% by mass
Water-soluble collagen 1.0% by mass
Hydrolyzed collagen 1.0% by mass
Seaweed extract 1.0% by mass
Lycopene-containing emulsion composition (composition prepared by the method described below) 0.1% by mass
Magnesium ascorbyl phosphate 1.0% by mass
Sodium hyaluronate 0.2% by mass
Citric acid 1.0% by mass
・Sodium citrate (appropriate amount) ・Water (remainder)

Example 5-2: Lotion
A lotion having the following composition was prepared in a conventional manner (total amount 100% by mass).
(composition)
Liposome composition of Example 3-2: 2.0% by mass
Arbutin 2.0% by mass
Dipotassium glycyrrhizinate 1.0% by mass
Dipropylene glycol 4.0% by mass
Polyoxyethylene methyl glucoside 1.0% by mass
1,3-butylene glycol 4.0% by mass
Polyethylene glycol 1.0% by mass
Ethanol 2.0% by mass
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Phenoxyethanol 0.3% by mass
Glycerin mono-2-ethylhexyl ether 0.2% by mass
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
N-acetyl-L-hydroxyproline 1.0% by mass
Water-soluble collagen 1.0% by mass
Hydrolyzed collagen 1.0% by mass
Seaweed extract 1.0% by mass
Lycopene-containing emulsion composition (composition prepared by the method described below) 0.1% by mass
Magnesium ascorbyl phosphate 1.0% by mass
Sodium hyaluronate 0.2% by mass
Citric acid 1.0% by mass
・Sodium citrate (appropriate amount) ・Water (remainder)

The lotions obtained in Examples 5-1 and 5-2 had good properties in terms of feel when used, fragrance, etc., and it was found that the liposome composition for cosmetics disclosed herein can be suitably used for preparing lotions.

The lycopene-containing emulsion compositions used in Examples 5-1 and 5-2 were prepared by the following method.

<Preparation of lycopene-containing emulsion composition>
(Preparation of Oil Phase Composition a)
The components having the following composition were weighed into a container and heated and mixed on a hot plate at 150° C. for 5 minutes with stirring to obtain an oil phase composition a.
(composition)
Tomato oleoresin (lycopene content 6% by mass) 2.8 parts by mass (Sunbright Co., Ltd., Lyc-O-Mato 6%)
Diglyceryl monostearate 0.3 parts by mass (Nikkol (registered trademark) DGMS, manufactured by Nikko Chemicals Co., Ltd., HLB: 5.0)
Mixture of glycerin tricaprylate and glycerin tricaprate: 11.3 parts by mass (manufactured by Kao Corporation, Coconard (registered trademark) MT, HLB: 1)
Mixed tocopherol 0.6 parts by mass (Riken Vitamin Co., Ltd., Riken E Oil 800)

(Preparation of aqueous phase composition a)
The following components were weighed into a container and heated and mixed in a thermostatic bath at 70° C. while stirring to obtain an aqueous phase composition a.
(composition)
Decaglyceryl oleate 10 parts by mass (Decaglyn 1-O, manufactured by Nikko Chemicals Co., Ltd.)
Glycerin 45 parts by weight Purified water 30 parts by weight

The obtained aqueous phase composition a was added to the oil phase composition a, and the mixture was stirred and mixed, and dispersed for a predetermined time using an ultrasonic homogenizer to obtain a crude dispersion.
Thereafter, the obtained crude dispersion was subjected to high-pressure emulsification at 200 MPa using an ultra-high-pressure emulsifier (Ultimaizer, manufactured by Sugino Machine Ltd.) to prepare a lycopene-containing emulsion composition (lycopene content: 0.17% by mass).

The resulting lycopene-containing emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (Otsuka Electronics Co., Ltd.), which was 52 nm.

Example 6-1: Jelly-like beauty essence
A jelly-like beauty essence having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-1: 2.0% by mass
Haematococcus algae extract 0.1% by mass
Astaxanthin-containing emulsion composition: 0.2% by mass
(Krill extract, composition prepared by the method described below)
Mixture of ceramide III and ceramide VI 1.0% by weight
Hydrolyzed collagen 1.0% by mass
Acetylhydroxyproline 1.0% by mass
Ethylhexylglycerin 0.1% by mass
Oleic acid 0.5% by mass
1,3-butylene glycol 1.0% by mass
Glycerin 2.0% by mass
Sucrose 0.1% by mass
Polyglyceryl-10 oleate 0.1% by mass
Polyglyceryl-2 stearate 0.1% by mass
Phenoxyethanol 0.2% by mass
Collagen 1.0% by mass
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Centella asiatica extract 0.01% by mass
Lecithin 0.1% by mass
Citric acid 1.0% by mass
Sodium citrate (appropriate amount) PEG-240, decyltetradeceth-20 and HDI copolymer (1.5% by weight)
・Damask rose flower oil (trace amount) ・Fragrance (trace amount) ・Water (balance)

Example 6-2: Jelly-like beauty essence
A jelly-like beauty essence having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-2: 2.0% by mass
Haematococcus algae extract 0.1% by mass
Astaxanthin-containing emulsion composition: 0.2% by mass
(Krill extract, composition prepared by the method described below)
Mixture of ceramide III and ceramide VI 1.0% by weight
Hydrolyzed collagen 1.0% by mass
Acetylhydroxyproline 1.0% by mass
Ethylhexylglycerin 0.1% by mass
Oleic acid 0.5% by mass
1,3-butylene glycol 1.0% by mass
Glycerin 2.0% by mass
Sucrose 0.1% by mass
Polyglyceryl-10 oleate 0.1% by mass
Polyglyceryl-2 stearate 0.1% by mass
Phenoxyethanol 0.2% by mass
Collagen 1.0% by mass
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Centella asiatica extract 0.01% by mass
Lecithin 0.1% by mass
Citric acid 1.0% by mass
Sodium citrate (appropriate amount) PEG-240, decyltetradeceth-20 and HDI copolymer (1.5% by weight)
・Damask rose flower oil (trace amount) ・Fragrance (trace amount) ・Water (balance)

The jelly-like beauty serums obtained in Examples 6-1 and 6-2 had good properties in terms of usability, fragrance, etc., and it was found that the liposome composition for cosmetics disclosed herein can be suitably used for preparing jelly-like beauty serums.

The astaxanthin-containing emulsion compositions used in Examples 6-1 and 6-2 were prepared by the following method.

<Preparation of astaxanthin-containing emulsion composition>
(Preparation of aqueous phase composition b)
The following components were dissolved for 1 hour while heating at 70° C. to obtain an aqueous phase composition b.
(composition)
Sucrose stearate (HLB: 16) 33.0g
Decaglyceryl monooleate (HLB: 12) 67.0g
Glycerin 450.0g
・Pure water 300.0g

(Preparation of Oil Phase Composition b)
The following components were dissolved for 1 hour while heating at 70° C. to obtain an oil phase composition b.
(composition)
・Krill extract 15.0g
・Mixed tocopherol 32.0g
(Riken Vitamin Co., Ltd., Riken E Oil 800)
・Medium chain fatty acid glyceride 93.0g
(Kao Corporation, Coconard (registered trademark) MT)
Lecithin 10.0g
(Riken Vitamin Co., Ltd., Resion (registered trademark) P, derived from soybeans)

The aqueous phase composition B obtained above was stirred (10,000 rpm) using a homogenizer (HP93, manufactured by SMT Co., Ltd.) while keeping the temperature at 70°C, and then the oil phase composition b was added to the aqueous phase composition b to obtain a preliminary emulsion.
The obtained preliminary emulsion was cooled to about 40°C and subjected to high-pressure emulsification at a pressure of 200 MPa using an Ultimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.) After that, it was filtered through a microfilter with an average pore size of 1 μm to prepare an astaxanthin-containing emulsion composition (astaxanthin content: 0.3% by mass).

The resulting astaxanthin emulsion composition was diluted to 1% by mass with Milli-Q water, and the particle size of the dispersed particles was measured using a particle size analyzer FPAR-1000 (Otsuka Electronics Co., Ltd.), which was found to be 58 nm.

Example 7-1: Sunscreen
A sunscreen agent having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-1: 2.0% by mass
Cyclopentasiloxane 20.0% by mass
Dimethicone 10.0% by mass
Titanium oxide 5.0% by mass
t-Butyl methoxybenzoylmethane 1.0% by mass
・HXMT-100ZA (Teika, average primary particle size 15 nm) 6.0% by mass
Aluminum hydroxide 1.0% by mass
Isostearic acid 0.5% by mass
Sorbitan sesquioleate 1.0% by mass
Dipotassium glycyrrhizinate 0.5% by mass
Magnesium ascorbyl phosphate 0.1% by mass
Krill extract 0.5% by mass
Water-soluble collagen 1.0% by mass
Citric acid 0.7% by mass
・Sodium citrate (appropriate amount) ・Tocopherol (0.5% by mass)
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Centella asiatica extract 0.01% by mass
Lecithin 0.1% by mass
Fragrance: Trace amount Methyl paraoxybenzoate: 0.15% by mass
Water Remainder

Example 7-2: Sunscreen
A sunscreen agent having the following composition was prepared by a conventional method (total amount 100% by mass).
(composition)
Liposome composition of Example 3-2: 2.0% by mass
Cyclopentasiloxane 20.0% by mass
Dimethicone 10.0% by mass
Titanium oxide 5.0% by mass
t-Butyl methoxybenzoylmethane 1.0% by mass
・HXMT-100ZA (Teika, average primary particle size 15 nm) 6.0% by mass
Aluminum hydroxide 1.0% by mass
Isostearic acid 0.5% by mass
Sorbitan sesquioleate 1.0% by mass
Dipotassium glycyrrhizinate 0.5% by mass
Magnesium ascorbyl phosphate 0.1% by mass
Krill extract 0.5% by mass
Water-soluble collagen 1.0% by mass
Citric acid 0.7% by mass
・Sodium citrate (appropriate amount) ・Tocopherol (0.5% by mass)
Oryzanol 0.01% by mass
Polyoxyethylene phytosterol 0.03% by mass
(Nikkol (registered trademark) BPS-20, manufactured by Nikko Chemicals Co., Ltd.)
Polyoxyethylene hydrogenated castor oil 0.2% by mass
(Average number of moles of oxyethylene added: 60)
Centella asiatica extract 0.01% by mass
Lecithin 0.1% by mass
Fragrance: Trace amount Methyl paraoxybenzoate: 0.15% by mass
Water Remainder

The sunscreens obtained in Examples 7-1 and 7-2 had good properties in terms of usability, fragrance, etc., and it was found that the liposome composition for cosmetics disclosed herein can be suitably used for preparing sunscreens.

Claims (9)

A liposome containing polyoxyethylene phytosterol, lecithin, and at least one of cholesterol and a phytosterol other than the polyoxyethylene phytosterol, and having an average particle size of 100 nm to 350 nm;
A liposome composition for cosmetics comprising: an internal aqueous phase that contains water, ethanol, and a cosmetic ingredient and is encapsulated in the liposome; and an external aqueous phase that contains water and ethanol and disperses the liposome,
the content of the polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is 0.05% by mass or more and 1% by mass or less;
The content of the lecithin relative to the total mass of the liposome composition for cosmetics is 0.3% by mass or more and 10% by mass or less,
the content of at least one of the cholesterol and the phytosterol other than the polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is 0.05% by mass or more and 0.7% by mass or less;
A liposome composition for cosmetics. The liposome composition for cosmetics according to claim 1, wherein the average number of moles of oxyethylene groups added to the polyoxyethylene phytosterol is 10 or more. 3. The liposome composition for cosmetics according to claim 1, wherein the content of the polyoxyethylene phytosterol relative to the total mass of the liposome composition for cosmetics is 0.1% by mass or more and 1% by mass or less . The liposome composition for cosmetics according to any one of claims 1 to 3, wherein the content of the ethanol relative to the total mass of the liposome composition for cosmetics is 0.2% by mass to 30% by mass. The liposome composition for cosmetics according to any one of claims 1 to 4, wherein the content of at least one of the cholesterol and the phytosterol other than the polyoxyethylene phytosterol is 0.1% by mass to 0.5% by mass relative to the total mass of the liposome composition for cosmetics. The liposome composition for cosmetics according to any one of claims 1 to 5, wherein the cosmetic ingredient is a water-soluble cosmetic ingredient. The liposome composition for cosmetics according to any one of claims 1 to 6, wherein the cosmetic ingredient is at least one selected from caffeine, pyridoxine hydrochloride, and nicotinamide. The liposome composition for cosmetics according to any one of claims 1 to 7, wherein the ratio of the total number of liposomes having a single lamellar structure to the total number of liposomes contained in the liposome composition for cosmetics is 70% or more. The liposome composition for cosmetics according to any one of claims 1 to 8, which is a cosmetic.