JP7671433B2 - Composition with hair growth and hair restoration function, its production method and use - Google Patents

Description

The present invention belongs to the field of hair growth and hair restoration technology, and in particular relates to a composition having hair growth and hair restoration functions, a preparation method, and uses.

Statistics show that one in four men and one in eight women experience hair loss. Also, according to one survey, the current hair loss rate for people born after 1990 exceeds 39.3%. Hair loss is already becoming more and more common, and is occurring at younger ages.

Although there are many products on the market that are advertised as having hair growth and hair care functions, these products are not ideal in terms of hair growth and hair care effects. In addition, most hair growth products are cleaning and protection products, and there are few other products, but cleaning and protection products must have cleaning functions, so they inevitably add chemical ingredients that damage hair, such as surfactants and conditioning agents, so there is an urgent need for a composition with hair growth and hair care functions that has good hair growth and hair care effects.

The purpose of the present invention is to invent a composition that is effective in promoting hair growth and hair development and has the function of promoting hair growth and hair development.

In order to achieve the above object, the present invention proposes the following technical solution:
The first technical solution of the present invention is a composition having hair growth and hair care functions, and the raw materials of the composition having hair growth and hair care functions include, in parts by weight, the following:
Further, the protein comprises low molecular weight peptides and amino acids, the low molecular weight peptides comprising, by weight, 500 to 1500 parts of bovine collagen peptides and 500 to 1500 parts of hydrolyzed collagen peptides, and the amino acids comprising, by weight, 20 to 50 parts of taurine.
Further, nutritional supplements include vitamin supplements and mineral supplements.
Additionally, the vitamin supplement includes, by weight, 0.2-10 parts vitamin A, 10-50 parts vitamin C, 1-10 parts vitamin E, and 10-50 parts yeast powder, and the mineral supplement includes, by weight, 1-10 parts ferrous fumarate and 0.2-10 parts zinc oxide.
Additionally, the powdered phytosterol ester is a β-sitosterol ester having the chemical formula C47O2H80.
Furthermore, the method for preparing the foxtail millet extract includes grinding foxtail millet, extracting it with water, concentrating the extract, and freeze-drying it to obtain the foxtail millet extract.
Additionally, the flavorings include, by weight, 100-500 parts erythritol and 100-500 parts grapefruit powder.

The second technical solution of the present invention: The method for preparing the composition having the above-mentioned hair growth and hair care function includes:
(1) weighing each ingredient by weight and sieving it;
A step (2) of mixing the nutritional enhancer and the seasoning measured in the step (1) to obtain a mixed raw material A;
Step (3) of mixing the protein and active ingredient measured in step (1) in a granulator, granulating the mixture using alcohol as a binder, and then fluidized bed drying and granulating the mixture in a granulator to obtain mixed raw material B;
Step (4) of mixing the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3) and sieving the mixture to obtain a composition having hair growth and hair care functions;
Includes.
Furthermore, in step (1), the sieve is a 40 to 100 mesh sieve.
Further, in step (2), the mixture is an equal volume increment mixture.
Furthermore, in step (3), the stirring speed is 200 rpm or more and 500 rpm or less, the amount of alcohol used is 280 to 480 parts by mass of alcohol with a concentration of 70 to 95%, sprayed within 2 to 10 minutes, the fluidized bed drying temperature is 51 to 58°C, the time is 25 to 35 minutes, and the sieving machine is a 20 mesh sieving machine.
Furthermore, in step (4), the sieve is a sieve of 40 to 100 mesh.

The third technical solution of the present invention is the application of the above-mentioned composition having hair growth and hair care functions in hair growth and hair care.
Compared with the prior art, the present invention has the following beneficial effects:
(1) A composition having hair growth and hair development functions that can shorten the time it takes for hair follicles to enter the anagen phase from the resting phase, accelerate hair growth time, and have a significant hair growth promotion effect;
(2) The composition having hair growth and hair development function prepared by the present invention has no toxicity or side effects and is effective in promoting the neogenesis of hair follicles.
(3) The present invention adopts fluidized bed granulation instead of oven drying, which has the advantages of more uniform fluidization, more uniform drying, less damage to the material surface, and coarser powder than oven drying. It has high thermal efficiency and energy saving effect, and can save 30-60% energy compared to oven drying.

The hydrolyzed collagen peptide of the present invention belongs to one of the necessary components of human skin, and can effectively guarantee the moisture of the skin and increase the firmness of the skin. The most important protein in hair is keratin, and collagen can possibly support and increase the hair growth protein in the body, which can strengthen hair, promote hair growth and prevent hair loss.
In addition, the present application selected two types of collagen peptides because one type is a collagen peptide extracted from animal skin and the other is a collagen peptide derived from bone, which have different amino acid components and peptide segments and can complement each other.
The taurine added in the present invention not only has the effect of repairing damaged hair, but also has the effect of increasing the production of hair growth factors and promoting hair growth.Taurine acts on the human hair cycle, can extend the growth period, create thick and strong hair, and can increase the production of the growth factor IGF-1.
The various vitamins added in the present invention have the effect of increasing nutrition of the scalp, promoting the metabolism of hair follicle cells, and further promoting hair growth.
The yeast powder added in the present invention contains vitamin H and niacin. Vitamin H, also known as biotin, is a nutrient necessary for the natural growth and development of the human body and for maintaining normal bodily functions. It can directly control the metabolism of hair follicle cells, and indirectly affect the development of hair follicles by affecting their intake and metabolism.
In addition, the niacin contained in yeast powder is also an essential nutrient for the human body, and plays an important role in promoting normal growth and development of the human body, promoting iron absorption and blood cell production, and maintaining normal skin function.
The present invention adds ferrous fumarate, elemental iron provides oxygen to every cell in the body, including the cells of the scalp and hair follicles.
The present invention adds zinc oxide, which is a cofactor for several enzymes in the body, an important component of antioxidant enzymes in the body, can enhance the activity of blood cells, and is a trace element necessary for hair growth and keratin synthesis.
The phytosterol ester powder added in the present invention is produced by esterification or transesterification between phytosterol and fatty acid. Sterol ester can be converted into sterol and fatty acid in the body, so its physiological functions include the physiological functions of both phytosterol and fatty acid. Phytosterol ester is easily absorbed by the human body, and its utilization efficiency is much higher than that of ordinary phytosterol, and it can prevent hair loss by suppressing the production of androgen.
The foxtail extract added in this invention is a rare oil extracted from foxtail, specifically milletin (chemical formula: C31H52O), which acts on the root of the hair follicle, promotes cell metabolism and proliferation, can maintain hair growth, nourish and repair damaged scalp, create a good foundation for healthy hair, and also has anti-inflammatory and anti-toxic effects.
The added Astragalus persicae powder according to the present invention is related to the kidney meridian, has the effect of filling the essence, and is effective in treating kidney deficiency alopecia and the like.
The mulberry powder added in the present invention has the effect of replenishing kidney and promoting vitality, and is suitable for the symptoms of senility, premature graying, hair loss, alopecia areata, and withering. Mulberry is both food and medicine. According to the records in the Chinese Pharmacopoeia, it has the effect of treating premature graying of hair. Mulberry is rich in proanthocyanidins, which can activate the stem cells in the bulge of hair follicles, transform the resting hair follicles into the growth phase, and promote hair regeneration.
The black-glanded rib Rowan fruit powder added in the present invention is rich in various vitamins, antioxidants and mineral elements, and has the effect of improving blood circulation in the head.

In order to more clearly describe the embodiments of the present invention or the technical solutions of the prior art, the drawings used in the embodiments are briefly described below. The drawings in the following description are some embodiments of the present invention, and those skilled in the art can obtain other drawings based on these drawings without creative labor.
FIG. 1 shows changes in the back hair of mice administered sample 3#. FIG. 2 shows changes in hair on the back of mice administered a low dose of a test substance. FIG. 3 shows changes in hair on the back of mice administered a high dose of a test substance. FIG. 4 is a graph showing changes in the back hair of mice administered with a positive control sample. FIG. 5 is a graph showing changes in the back hair of mice administered with a negative control sample. FIG. 6 is a statistical graph showing the number of newly generated hair follicles under the microscope for the administration sample 3#, the low-dose sample, the high-dose sample, the positive control sample and the negative control sample. FIG. 7 shows HE staining images of administered sample 3#, (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days. FIG. 8 shows HE staining images of mouse skin after administration of a low dose of a sample, where (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days. FIG. 9 shows HE staining images of mouse skin after administration of a high dose of a sample, where (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days. FIG. 10 shows HE staining images of the administered positive control sample, (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days. FIG. 11 shows HE staining images of the administered negative control sample, (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.

Various exemplary embodiments of the present invention will now be described in detail, but this detailed description should not be understood as limiting the present invention, but as a more detailed description of some aspects, features, and embodiments of the present invention. It should be understood that the terms described in the present invention are merely for describing particular embodiments, and are not intended to limit the present invention.
It is also to be understood that for any numerical ranges herein, each intermediate value between the upper and lower limits of the range is specifically disclosed. Each smaller range between a stated value or intermediate value within a stated range and any other stated value or intermediate value within a stated range is also included in the invention. The upper and lower limits of these smaller ranges may independently be included or excluded within the range.
Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention relates. The present invention describes only preferred methods and materials, but any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents described herein are incorporated by reference to disclose and describe the methods and/or materials related to the documents. In case of conflict with any of the incorporated documents, the contents of the specification shall prevail.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present specification without departing from the scope or spirit of the present invention. Other embodiments will be apparent to those skilled in the art from the present specification. The description and embodiments of the present invention are illustrative only.
As used herein, the terms "including,""including,""having,""including," and the like are all open-ended terms, i.e., meaning including, but not limited to.
In the embodiments of the present invention, "parts" are "parts by weight" unless otherwise specified.

In the following examples, the powdered plant sterol ester is prepared according to patent CN201410445798.0.
In the following embodiment, the method for preparing the foxtail millet extract is as follows:
1) Put the millet into a hot air drying box at 37℃ and dry for 24 hours, then crush it through an 80-mesh sieve.
2) Step 1) The sieved millet and water were mixed in a liquid ratio of 1:10 and extracted at 50℃ for 1 hour using a vortex. Extraction was then repeated twice, and the filtrate and residue were collected.
3) The filtrate obtained in step 2) is concentrated to a solid content of 25-35% to obtain a concentrated liquid.
4) A foxtail millet extract obtained by freeze-drying the concentrate obtained in step 3), the parameters of which are to keep the moisture content below 6%.
The mulberry powder, black-gland-rib rowan fruit powder and yellow ash powder described in the following embodiments are commercially available products, and the proanthocyanidin content in the mulberry powder and black-gland-rib rowan fruit powder is 10%, and the yellow ash polysaccharide content in the yellow ash powder is 15%.
In the following embodiments, bovine collagen peptides and hydrolyzed collagen peptides are commercially available products, both of which are food ingredients with a protein content of 95%.
The vitamin A described in the following embodiments is commercially available and is a food-grade additive with an active substance content of 325,000 IU/g.
In the following embodiment, the Vitamin C is a commercially available, food-grade additive with an active substance content of 97%.
In the following embodiments, taurine and ferrous fumarate are commercially available and are both food-grade additives with 99% active substance content.
In the following embodiment, Vitamin E is a commercially available, food-grade additive with an active ingredient content of 50%.
The yeast powder described in the following embodiments is a commercially available product, and contains 40 mg/g of niacin and 300 ppm of biotin, both of which are active substances.
In the following embodiments, the term "equal increment mixing" means that the least amount of the ingredient is mixed uniformly with an equal amount of another ingredient in equal increments, and then an equal amount of the other ingredient is added after mixing and mixed uniformly, and this double increment process is continued until all ingredients are mixed.

(1) Weigh out each ingredient based on the weight parts listed in 1# of Table 1, and sieve through a 40 mesh sieve.
(2) The nutritional enhancer and seasoning measured in step (1) are mixed in equal amounts to obtain mixed raw material A.
(3) The protein and active ingredients measured in step (1) are stirred in a granulator, granulated using alcohol as a binder, then fluidized-bed dried and sized in a 20-mesh granulator to obtain mixed raw material B. The stirring speed is set to 200 rpm, the amount of alcohol used is 280 parts of 70% alcohol within 2 minutes, the fluidized-bed drying temperature is set to 51°C, and the drying time is set to 25 minutes.
(4) The mixed raw material A obtained in step (2) is mixed with the mixed raw material B obtained in step (3) and passed through a 40 mesh sieve to obtain a composition having hair growth and hair care functions.

(1) Weigh out each ingredient based on the weight parts listed in #2 in Table 1, and sieve through a 100-mesh sieve.
(2) The nutritional enhancer and seasoning measured in step (1) are mixed in equal amounts to obtain mixed raw material A.
(3) The protein and active ingredients measured in step (1) are mixed in a granulator, granulated using alcohol as a binder, then fluidized-bed dried and sized in a 20-mesh granulator to obtain mixed raw material B. The mixing speed is 500 rpm, the amount of alcohol is 480 parts of 95% alcohol within 10 minutes, the fluidized-bed drying temperature is 58°C, and the drying time is 35 minutes.
(4) Mix the mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3) by sieving through a 100 mesh sieve to obtain a composition having hair growth and hair care functions.

(1) Weigh out each ingredient based on the weight parts listed in 3# in Table 1, and sieve it through a 60-mesh sieve.
(2) The nutritional enhancer and seasoning measured in step (1) are mixed in equal amounts to obtain mixed raw material A.
(3) The protein and active ingredients measured in step (1) are mixed in a granulator, granulated using alcohol as a binder, then fluidized-bed dried and sized in a 20-mesh granulator to obtain mixed raw material B, which is sprayed with 300 parts of 85% alcohol within 5.5 minutes at a mixing speed of 350 rp/min and a fluidized-bed drying temperature of 55°C for 30 minutes.
(4) The mixed raw material A obtained in step (2) and the mixed raw material B obtained in step (3) are mixed and passed through a 60 mesh sieve to obtain a composition having hair growth and hair care functions.

Comparative Example 1

The difference from Example 3 is that in step (1), each raw material was weighed out according to the weight amount shown in 4# in Table 2 and sieved through a 60 mesh screen.

Comparative Example 2

The difference from Example 3 is that in step (1), each raw material was weighed out according to the weight amount shown in 5# in Table 2 and sieved through a 60 mesh sieve.

Comparative Example 3

The difference from Example 3 is that in step (1), each raw material was weighed out according to the weight amount shown in 6# in Table 2 and sieved through a 60 mesh sieve.

Comparative Example 4

The difference from Example 3 is that in step (1), each raw material was weighed according to the weight amount shown in 7# in Table 2 and sieved through a 60 mesh sieve.

Comparative Example 5

The difference from Example 3 is that in step (1), each raw material was weighed out according to the weight amount shown in 8# in Table 2 and sieved through a 60 mesh sieve.

Comparative Example 6

The difference from Example 3 is that in step (1), the core raw material was weighed out in the parts by weight shown in Table 2 (number 9) and sieved through a 60 mesh sieve.

Comparative Example 7

The difference from Example 3 is that in step (1), each raw material was weighed out according to the weight amount shown in 10# of Table 2 and sieved through a 60 mesh screen.

Comparative Example 8

The difference from Example 3 is that in step (1), each raw material is weighed out by weight as shown in 11# of Table 2 and sieved through a 60 mesh sieve.

Comparative Example 9

The difference from Example 3 is that in step (1), each raw material is weighed out according to the weight amount shown in #12 of Table 2 and sieved through a 60 mesh sieve.

Comparative Example 10

The difference from Example 3 is that mulberry flour was replaced with black bean powder in step (1).

Effectiveness Verification 1. Effectiveness Verification of the Prescription (1) Selection of Subjects Select successful subjects based on the inclusion and exclusion criteria.

1. Selection Criteria (1) Healthy male or female, aged 25-45 years old.
Hair length is 5 to 40 cm.
(2) Those who suffer from mild hair thinning and have lost more than 10 hairs after 60 combing sessions, and who still have more than 10 hairs after a two-week preparation period.
(3) Those who have not undergone any special hair treatments such as coloring, perming, or styling within the past month.
2. Exclusion Criteria
(1) Pregnant or breastfeeding women or those planning to become pregnant in the near future.
(2) Persons with mental or heart illness, or long-term sleep or emotional regulation disorders.
(3) A person who has used cosmetics with hair loss prevention effects or other products with the effect of preventing hair loss or promoting hair growth within the past three months.
(4) Subjects who have taken or used topically any drug that affects hair growth for nearly six months.
3. Subject Restrictions (1) During the screening and study period, subjects were not able to wash their hair within 48±4 hours before each visit, and the time period during which they did not wash their hair before each visit was basically consistent. They were not able to comb their hair by themselves on the day of the visit.
(2) During the study period, participants were not allowed to have their hair cut within 2 weeks prior to each evaluation visit.
(3) During the trial period, you may not engage in any hair care or styling procedures or receive any hair loss prevention or hair growth treatments.
(4) During the study period, participants should maintain their original lifestyle and avoid large emotional fluctuations.

(II) Test substances Test samples: compositions prepared in the proportions of Examples 1 to 3 and Comparative Examples 1 to 10.
Method of use: The test samples were randomly distributed to the subjects, and the subjects were given instructions on how to use the samples to ensure that they used the test objects correctly during the test period. The test objects were required to be used for 12 weeks, and the subjects were required to record the time of use and any discomfort or adverse reaction symptoms during the use process during the test period.

(3) Testing Equipment Comb: The comb teeth have appropriate density (tooth spacing 0.9-1.1mm), the comb teeth length is 2.0-3.0cm, and the comb length is 10cm or more (not including the comb handle). Combs of the same specifications and materials must be used throughout the entire testing process, and disinfected after each use in accordance with the relevant requirements in the Disinfection Technical Specification for Medical Institutions (WS-T 367-2012).

(4) Study procedure Subjects were recruited as needed, and the number of hair loss was counted and recorded using the 60-times hair comb method.
Those who passed the test were given a two-week preparation period, during which a 0.9% sodium chloride solution was used. After the preparation period, the combing method was repeated 60 times, and those with 10 or more hairs lost were allowed to move on to the official test.
Subjects selected for the formal trial were divided into 12 groups using a stratified randomization method to ensure balance of factors that may influence the trial results, such as gender, age, hair length, and severity of hair loss.
Selected subjects will undergo a baseline evaluation of hair growth before using the product, including hair loss counts and records, and will then undergo the same evaluation and testing again 4, 8, and 12 weeks after using the product.

(5) Hair loss count: At each visit, trained staff will comb the subject's hair using the combing method 60 times, and count and record the amount of hair that has fallen out.

(6) Test Results The results of the formulation effect verification are shown in Table 3.

(7) Conclusion According to the data in Table 3, the difference in the number of hair removal times after 12 weeks in Examples 1 to 3 (number of hair removal times before using the product - number of hair removal times after 12 weeks using the product) is higher than that in Comparative Examples 1 to 10, and the hair removal prevention effect of Examples 1 to 3 is superior to that of Comparative Examples 1 to 10. In addition, among Examples 1 to 3, the hair removal prevention effect of Example 3 is superior to that of Examples 1 and 2.

II. Dose-effect verification (1) Experimental materials and methods
1. Grouping of mice:
Fifty C57BL/6J mice (SPF grade) aged 38-40 days and weighing 15-20 g (provided by the Experimental Animal Center of Southern Medical University, China) were reared for three days in an adaptive manner and then randomly divided into five groups of 10 mice each. The hair on the backs of the mice was shaved 24 hours prior to administration to induce the hair to go from the telogen phase to the anagen phase.
2. Sample configuration:
1.54 g of the composition prepared in Example 3 is completely dissolved in 20 mL of water to obtain sample 3#.
0.77 g of the composition prepared in Example 3 is completely dissolved in 20 mL of water to obtain a low-dose sample.
3.08 g of the composition prepared in Example 3 is completely dissolved in 20 mL of water to obtain a high dose sample.
Fenasteride solids are placed in a 0.2% finasteride solution to provide a positive control sample.
Sodium chloride solid is placed in a 0.9% sodium chloride solution to provide a negative control sample.
3. Administration Test specimen 3# and the control specimen of group 4 were administered to five groups of mice, respectively, at 0.2 mL once a day for 8 weeks.

(2) Experimental Results
1. Changes in Mouse Body Weight During the measurement, the body weight of each mouse was measured and recorded every 7 days, and expressed as the average ± standard deviation. The specific results are shown in Table 4.
The data in Table 4 above shows that the weight change trends of the five groups of mice were almost consistent throughout the entire testing process, and all the mice were normal.

2. Changes in mouse skin and time until hair growth The skin and hair growth at the hair removal site of mice in each group were observed daily, and the specific hair growth status is shown in Figures 1 to 5 (photographs were taken every 7 days). The time when the skin color at the hair removal site of each mouse changed from pink to black and the time when hair started to grow were recorded and expressed as the average value ± standard deviation. The specific results are shown in Table 5.
FIG. 1 shows changes in the back hair of mice administered sample 3#.
FIG. 2 shows changes in hair on the back of mice administered a low dose of a test substance.
FIG. 3 shows changes in hair on the back of mice administered a high dose of a test substance.
FIG. 4 is a graph showing changes in the back hair of mice administered with a positive control sample.
FIG. 5 is a graph showing changes in the back hair of mice administered with a negative control sample.

From the above figure, it can be seen that in sample 3#D14, there were two mice with gray skin and two mice with hair, and in D21, there were four mice with obvious hair, one mouse with hair growing on 60% of its back, and three mice with hair growing on its entire back.
In the low-dose sample, one mouse had gray skin at D14, and five mice had hair at D21, of which two had hair over 70% of their backs and two had hair over 80% of their backs.
In the high-dose sample, we found that five mice had hair on their backs at D14 and five mice had hair on their backs at D21, of which three mice had hair over 90% of their backs and two mice had hair over the entire back.
In the positive control samples, at D14, 4 mice had gray skin and 1 mouse was clearly hairy, and at D21, 6 mice were found to have hairy areas covering 90% of their backs.
In the negative control samples, 2 mice had gray skin at D14, 3 mice had hair growth on 70% of their backs, and 2 mice had hair growth on 90% of their backs at D21.

From the data in Table 5 above, it can be seen that compared with the negative control test strip, test strip 3#, high dose test strip and positive control test strip shortened the time to enter the anagen phase and hair growth to a certain extent, among which, sample 3# more significantly shortened the time from the hair follicle entering the anagen phase to hair growth.

3. Mouse Dorsal Skin Histological Examination
On the 7th, 14th, 28th, and 56th days, the mice were humanely killed 24 hours after administration. Two mice were killed each time, and skin samples were taken from the same areas of the shaved areas, fixed, and prepared as usual. They were stained with HE staining, and the number of newly formed hair follicles observed under a microscope was expressed as an average value. The specific results are shown in Tables 6 to 9 and Figure 6.

The HE staining patterns of test specimen 3#, the low-dose test specimen, the high-dose test specimen, the positive control test specimen and the negative control test specimen are shown in Figures 7 to 11.
FIG. 7 shows HE staining images of sample 3#, (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.
FIG. 8 shows HE staining images of low-dose samples, where (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.
FIG. 9 shows HE staining images of high-dose samples, (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.
FIG. 10 shows HE staining images of positive control test pieces, where (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.
FIG. 11 shows HE staining images of negative control samples, where (a) is a histological HE staining image of mouse skin after 7 days, (b) is a histological HE staining image of mouse skin after 14 days, (c) is a histological HE staining image of mouse skin after 28 days, and (d) is a histological HE staining image of mouse skin after 56 days.

From Figure 6 to Figure 11, the histological examination of the mouse back skin showed that the number of new hair follicles in the negative control group showed a downward trend throughout the entire experimental period on D7, D14, and D28, and reached a peak on D7. However, in the 3# high-dose group, the number of new hair follicles reached a maximum on D14, and the number of new hair follicles on D28 was 0, while the number of new hair follicles in the positive control group peaked on D7 and showed a downward trend throughout the experimental period.
In the negative control group, the number of newly generated hair follicles on D7 and D14 was all 3, which was lower than the positive control group, and the number of newly generated hair follicles in the negative control group on D28 was 0.
The number of hair follicles regenerated in the low-dose group on D7 and D14 was basically the same as that in the negative control group, and the number of hair follicles regenerated on D28 was 3#, which was higher than that of the negative control group.
3# The number of hair follicle neogenesis on D7, D14, and D28 was significantly higher than that of the negative control group. The number of hair follicle neogenesis on D7 was 48.40% higher than that of the negative control group, and the number of hair follicle neogenesis on D14 reached its peak and was 218.00% higher than that of the negative control group.
The high-dose group had higher hair follicle neogenesis numbers on D7 and D14 than the negative control group, with the D7 hair follicle neogenesis number being 19.36% higher than the negative control group, the D14 hair follicle neogenesis number being 32.15% higher than the negative control group, and the D28 hair follicle neogenesis number and the negative control group hair follicle neogenesis number were all 0.
The positive control group peaked on D7 and showed a decreasing tendency after D14, and the number of new hair follicles on D28 and in the negative control group were all zero.

The composition of the present invention has no harmful side effects, promotes the metabolism and proliferation of hair follicle cells, promotes the creation of new hair follicles, shortens the time it takes for hair follicles to enter the anagen phase from the resting phase, has a significant hair growth promoting effect, strengthens the tightness of hair within the scalp, and can prevent hair loss and settling.

As stated above, the scope of protection of the present invention is not limited to the better specific embodiment of the present invention, and any technical person familiar with the technical field that is equivalently replaced or modified within the technical scope of the disclosure of the present invention based on the technical solution of the present invention and its inventive idea shall be included in the scope of protection of the present invention.

Claims (3)

The composition having a hair growth and hair care function includes ingredients, in parts by weight, as shown in Table 1 below:
A composition having hair growth and hair care functions, characterized by containing any one of the ingredients described in 1#, 2# or 3# . (1) weighing each ingredient by weight and sieving it;
A step (2) of mixing the nutritional enhancer and the seasoning measured in the step (1) to obtain a mixed raw material A;
Step (3) of mixing the protein and active ingredient measured in step (1) in a granulator, granulating the mixture using alcohol as a binder, and then fluidized bed drying and granulating the mixture in a granulator to obtain mixed raw material B;
and (4) mixing the mixed raw material A obtained in step (2) with the mixed raw material B obtained in step (3) and sieving the mixture to obtain a composition having hair growth and hair care functions. The method according to claim 2, wherein in step (1), the sieve is a 40-100 mesh sieve ; in step (3) , the stirring rotation speed is 200 rpm or more and 500 rpm or less; the amount of alcohol used is 280-480 parts by mass of alcohol having a concentration of 70-95%, sprayed within 2-10 minutes; the fluidized bed drying temperature is 51-58°C, the time is 25-35 minutes; the sieve is a 20 mesh sieve; and in step (4), the sieve is a 40-100 mesh sieve.