JP7795755B2 - topical skin preparations - Google Patents

Description

The present invention relates to an external skin preparation that is highly safe and stable in vivo and contains vitamin B6 (excluding vitamin B6 salts and derivatives) as an active ingredient.

The use of vitamin B6 as an active ingredient in cosmetics has been known, for example, from Patent Documents 1 to 7. However, vitamin B6 as an ingredient in topical skin preparations is almost always used in the form of hydrochloride salts for stability reasons. However, hydrochloride salts are highly acidic, which poses safety issues. Furthermore, adjusting the pH of compositions containing hydrochloride salts using pH adjusters during industrial processing has also created the problem of large amounts of salt being generated.

Japanese Patent Application Publication No. 55-049306 Japanese Patent Publication No. 58-180409 Japanese Patent Application Publication No. 61-254510 Japanese Patent Application Publication No. 63-022510 Japanese Patent Application Publication No. 10-194914 Japanese Patent Application Publication No. 10-279431 Japanese Patent Application Laid-Open No. 2004-091370

In light of the above, the present invention aims to provide a new use for vitamin B6 (excluding salts or derivatives of pyridoxine) that is highly safe.

The present invention is a fat accumulation inhibitor containing vitamin B6 as an active ingredient.
The present invention is an intracellular active oxygen production inhibitor containing vitamin B6 as an active ingredient.
The present invention is a prostaglandin E2 synthesis inhibitor containing vitamin B6 as an active ingredient.

The present invention is a lipid droplet accumulation inhibitor, an intracellular reactive oxygen production inhibitor, or a prostaglandin E2 synthesis inhibitor that contains vitamin B6 (excluding vitamin B6 salts or derivatives) as an active ingredient, and can provide new efficacy as an ingredient in safe topical skin preparations.

FIG. 1 shows the results of a test evaluating the inhibitory effect of the active ingredient according to the present invention on perilipin 1 gene expression. FIG. 1 shows the results of a test evaluating the inhibitory effect of the active ingredient according to the present invention on intracellular ROS production. FIG. 1 is a diagram showing the results of a test evaluating the prostaglandin E2 production inhibition of the active ingredient according to the present invention.

Preferred embodiments of the present invention will now be described in detail.
The present invention relates to a composition containing vitamin B6 (excluding vitamin B6 salts or derivatives). Commercially available vitamin B6 can be used as the vitamin B6 of the present invention. However, it is also possible to use a desalted product obtained by removing the hydrochloride from pyridoxine hydrochloride by ion exchange treatment. Examples of ion exchange treatment include electrodialysis using an ion exchange membrane and a substitution method using an ion exchange resin. Furthermore, from the viewpoint of efficient dehydrochlorination, a dehydrochlorination method using an anion exchange membrane and an electrolytic reduction device can also be used.

The ion exchange method involves passing a solution of hydrochloride through a column packed with a strongly basic anion exchange resin to remove the chloride. The resulting solution is a dehydrochlorinated aqueous solution.

In addition, in the present invention, in order to improve the stability of cosmetics containing vitamin B6, one or more selected from inorganic acids or their salts, organic acids or their salts, chelating agents, and plant-, seaweed-, and animal-derived ingredients may be blended. Examples of inorganic acids or their salts include sulfurous acid or its salts, phosphoric acid or its salts, and other inorganic salts. Examples of organic acids or their salts include citric acid or its salts, malic acid or its salts, tartaric acid or its salts, oxalic acid or its salts, lactic acid or its salts, sorbic acid or its salts, fumaric acid or its salts, and phthalic acid or its salts. Examples of chelating agents include edetic acid or its salts, ethylenediaminetriacetic acid or its salts, gluconic acid or its salts, phytic acid or its salts, pyrophosphoric acid or its salts, polyphosphoric acid or its salts, metaphosphoric acid or its salts, and hydroxyethanediphosphonic acid or its salts.

In addition, in the present invention, the inorganic acid salt may be any salt that is physiologically acceptable (potassium, sodium, calcium, magnesium, iron, zinc, etc.). Examples of the sulfite salt include sodium bisulfite, sodium pyrosulfite or sodium sulfite, potassium bisulfite, potassium sulfite, calcium bisulfite, calcium sulfite, magnesium bisulfite, and magnesium sulfite, but the present invention is not limited thereto. Similarly, the phosphate salt may be any salt that is physiologically acceptable (potassium, sodium, calcium, magnesium, iron, zinc, etc.).

The organic acid salts used in the present invention may be any physiologically acceptable salt (potassium, sodium, calcium, magnesium, iron, zinc, etc.). For example, citrates include sodium citrate, disodium citrate, trisodium citrate, potassium citrate, dipotassium citrate, and tripotassium citrate, but the present invention is not limited to these. Similarly, the above-mentioned malate, tartrate, oxalate, lactate, sorbate, pyruvate, fumarate, and phthalate may be any physiologically acceptable salt (potassium, sodium, calcium, magnesium, iron, zinc, etc.).

When the composition of the present invention is incorporated into a topical skin preparation, in addition to the above-mentioned essential components, ingredients commonly used in cosmetics, such as oily ingredients, surfactants (synthetic and natural), moisturizers, anti-inflammatory agents, thickeners, preservatives/bactericides, anti-acne agents, cell activators, powder ingredients, UV absorbers, antioxidants, whitening agents, anti-wrinkle agents, pigments, fragrances, etc., can be incorporated as needed.

Here, examples of oily ingredients include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. Examples include hydrocarbons such as cocos and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, and polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as phenyl alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, and N-acylamidopropyl-N',N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

Emulsifiers and/or emulsifying aids that can be used include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, soy-derived water-soluble polysaccharides, soy-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

Humectants include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, etc., as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, and various amino acids and their derivatives.

Thickeners include, for example, ingredients derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid/methacrylic acid copolymer; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or its derivatives.

Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and ammonium bromide. These include chylisoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark dry distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

Cell activators include pantothenyl alcohol, menthol, dl-menthol, and gamma-oryzanol.

Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, and pyridoxine dicaprylate.

Examples of powder ingredients include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, grain powder (rice, wheat, corn, millet, etc.), and bean powder (soybean, adzuki bean, etc.).

Examples of ultraviolet absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), etc.

Further examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate).

Resorcinol derivatives include, for example, 4-n-butylresorcinol and 4-isoamylresorcinol; 2,5-dihydroxybenzoic acid derivatives include, for example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

Furthermore, the following animal-, plant-, or microbial-derived ingredients can also be used in combination. For example, collagen or its hydrolysate, yeast extract or hydrolysate, lactic acid bacteria culture, grasses, cruciferous plants, Theaceae, Rosaceae, Paeoniaceae, Rutaceae, Amaranthaceae, Zosteraea, Leguminosae, Asteraceae, Leguminosae, Malvaceae, Gentianaceae, Lamiaceae, Nelumbosacaceae, Cucurbitaceae, Araliaceae, Solanaceae, Bignoniaceae, Actinidiaceae, Mulberry, Iridaceae, Campanulaceae, Oleaceae, Actinidiaceae, Mulberry, Rhamnaceae, Orchidaceae, and Anacardiaceae. Examples of such extracts include extracts of one or more plants selected from the family Garcinia, Valencaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

Preferred ingredients derived from plants of the Poaceae family include rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice fermentation liquid, sake lees extract derived from sake, bamboo shoot skin extract from Madake or Moso bamboo, and fermented Job's tears seeds. Furthermore, preferred Brassicaceae plants include extracts of seeds from Hakugai, Ogai, or Kokugai, or their hydrolysates or fermented products. Preferred ingredients derived from plants of the Theaceae family include green tea (e.g., Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuuki) and black tea (e.g., Darjeeling, Assam, Ceylon, Earl Grey, and honey-scented black tea). Preferred ingredients derived from plants of the Rosaceae family include Damask rose flower extract, peach flower, leaf, or immature fruit extract, apricot fruit or seed extract, strawberry flower extract, and cherry blossom flower or leaf extract. Furthermore, as components derived from Paeoniaceae plants, extracts of peony root or flower and peony flower or root are preferred. As components derived from Amaranthaceae plants, Salicornia extract is particularly preferred. As components derived from Zosteraea plants, extracts of Zostera marina or Zostera kohlrabi are particularly preferred. As components derived from Leguminosae plants, extracts of white soybean or black soybean or their hydrolysates, fermented soy milk, adzuki bean extract, red clover extract, and pueraria lobata root extract are particularly preferred. As components derived from Asteraceae plants, burdock root extract, sunflower sprout extract, Arctium gracilis extract, arnica extract, and chamomile flower extract are particularly preferred. As components derived from Malvaceae plants, fermented products of hibiscus, rose of sharon, or hibiscus are preferred. As components derived from Gentianaceae plants, gentian extract is preferred. As components derived from Lamiaceae plants, perilla extract and barberry fruit extract are preferred. As components derived from plants of the Nelumbo family, particularly preferred are lotus flower or lotus seed extracts, or fermented lotus seed products. As components derived from plants of the Cucurbitaceae family, particularly preferred is loofah extract. As components derived from plants of the Araliaceae family, preferred is an extract or fermented product of Panax ginseng. As components derived from plants of the Solanaceae family, extracts of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) are exemplified. As components derived from plants of the Bignoniaceae family, preferred is Pau d'Arco bark extract. As components derived from plants of the Actinidiaceae family, preferred is immature kiwi extract. As components derived from plants of the Moraceae family, preferred is Morus alba extract, mulberry fruit extract, or fig fruit or bark extract. As components derived from plants of the Rhamnaceae family, preferred is jujube fruit extract. Furthermore, as components derived from plants of the Iridaceae family, preferred is saffron. As components derived from plants of the Campanulaceae family, preferred is an extract or hydrolysate of Codonopsis globulus root. As components derived from plants of the Anacardiaceae family, preferred is mango fruit extract. As a component derived from a plant of the family Garciniae, mangosteen fruit extract is particularly preferred. As a component derived from a plant of the family Valenciaceae, cherimoya fruit extract is particularly preferred. As a component derived from a plant of the family Rutaceae, Satsuma mandarin, bergamot fruit extract, grapefruit or banpeiyu fruit (including immature fruits), or Japanese pepper seed extract is particularly preferred. As a component derived from a plant of the family Liliaceae, extracts of daylilies, daylilies, Casablanca lilies, Madonna lilies, or Japanese lilies are particularly preferred. As a component derived from a plant of the family Crassulaceae, extracts or fermented products of Rhodiola rosea (Rhodiola rosea) are particularly preferred. As a component derived from a plant of the family Oleaceae, jasmine flower extract is particularly preferred. As a plant of the family Cupressaceae, Juniper fruit extract is particularly preferred. As a component derived from a plant of the family Myrtaceae, guava leaf extract is particularly preferred. As a plant of the family Orchidaceae, extracts of Bletilla serrata (white orchid) root are particularly preferred. As a component derived from a plant of the Convolvulaceae family, a sweet potato extract or its fermented product, or a sweet potato shochu lees extract or its fermented product is preferred. Furthermore, as a plant of the Asparagaceae family, asparagus (green asparagus and white asparagus) is preferred. As a component derived from seaweed of the Laminaria family, kelp extract is particularly preferred, as a component derived from seaweed of the Mirinaceae family, katamen kyrinsai extract is preferred, and as a component derived from seaweed of the Ulva family, Ulva pertusa extract is particularly preferred. As a component derived from seaweed of the Funoriaceae family, funori extract is particularly preferred.

Next, the present invention will be explained in more detail using production examples, formulation examples, and test examples, but the present invention is not limited to these. Note that, below, all parts mean parts by weight, and all % means % by weight.

Preparation Example 1: Preparation of Vitamin B6 100 g of pyridoxine hydrochloride was dissolved in 900 g of purified water. This hydrochloride aqueous solution was passed through a strongly basic anion exchange resin column to remove hydrochloric acid. The solution was concentrated to dryness to obtain 6 g of pyridoxine (vitamin B6).

Test Example 1. Evaluation test of perilipin synthesis inhibitory effect The sebum secretion inhibitory effect of vitamin B6 according to the present invention is evaluated below by its inhibitory effect on the synthesis of perilipin 1, a protein present around lipid droplets in adipocytes. It is known that a decrease in perilipin 1 leads to a decrease in the number and size of lipid droplets, thereby inhibiting sebum secretion. Therefore, the inhibitory effect on perilipin 1 synthesis is evaluated.
[Test method]
Preadipocytes (3T3-L1) were seeded in Dulbecco's modified Eagle's MEM medium, to which IBMX, DEX, and insulin were added for differentiation induction. The medium was then replaced with a medium containing vitamin B6 (hereinafter referred to as the "sample of the present invention") or pyridoxine hydrochloride (hereinafter referred to as the "comparison sample") prepared in Preparation Example 1. After 24 hours of culture, the cells were harvested using Trizol reagent, and total RNA was extracted by standard methods. cDNA was synthesized by reverse transcription, and the expression level of the Perilipin1 gene relative to β-Actin was calculated by RT-PCR.

The results of Test Example 1 are shown in FIG.
As shown in Figure 1, it was confirmed that the sample of the present invention has a significantly superior inhibitory effect on Perilipin1 gene (PLIN1) expression compared to the comparative sample. This result suggests that the sample of the present invention has the effect of suppressing intracellular fat accumulation.

Test Example 2. Intracellular ROS Inhibition Test Normal human epidermal keratinocytes (NHEK) were prepared in HuMedia-KG2 medium at a density of 8 x ¹⁰ cells/mL. 100 μL of the culture was seeded onto a 96-well microplate and incubated at 37°C under 5% carbon dioxide and saturated steam. After 24 hours, the culture medium containing the sample was supplemented with pyridoxine base or pyridoxine hydrochloride at the specified concentration. After 48 hours, the supernatant was removed from the test group, and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-2DA) was incorporated into the cells. The cells were then washed with HBSS and irradiated from the bottom of the incubator with approximately 50 mJ/ ^cm2 of UV light using a UV-B lamp (Philips TL20W/12RS). Fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) was measured using a fluorescent plate reader (Fluoroscan Ascent, Thermo Labsystems) and used as the amount of oxidative damage. Then, Hoechst 33342 was incorporated and DNA staining was performed. Fluorescence intensity (excitation wavelength 355 nm, absorption wavelength 460 nm) was measured using a fluorescent plate reader (Fluoroscan Ascent, Thermo Labsystems) to determine the amount of DNA. After measurement, the amount of oxidative damage per amount of DNA was calculated.

The results of Test Example 2 are shown in FIG.
As shown in Figure 2, it was confirmed that the sample of the present invention has a significantly superior inhibitory effect on ROS production in epidermal cells compared to the comparative sample. This result suggests that the sample of the present invention can efficiently suppress oxidative stress in cells.

Test Example 3. Prostaglandin E2 production inhibition evaluation test [Test method]
SIRC cells were seeded at 5 x ¹⁰ cells/well in a 96-well plate and cultured until subconfluent. The medium was then replaced with one containing pyridoxine base or pyridoxine hydrochloride. After one day, the cells were irradiated with approximately 100 mJ/ ^cm2 of ultraviolet (UV-B) light from the bottom of the incubator using a UV-B lamp (Philips TL20W/12RS). After 24 hours of culture, the culture supernatant was aliquoted and PGE2 was quantified using an EIA kit (Caiman Chemical).

The results of Test Example 3 are shown in FIG.
As shown in Table 3, it was confirmed that the sample of the present invention has a significantly superior inhibitory effect on prostaglandin E2 production compared to the comparative sample. This result suggests an excellent anti-inflammatory effect.

Formulation example 1. Lotion [Ingredients] Parts Eucalyptus oil 0.2
Polyoxyethylene (5.5) Cetyl Alcohol 5.0
Compound of Preparation Example 1 0.1
Tocopherol acetate 0.02
Dipotassium glycyrrhizinate 0.5
Monoammonium glycyrrhizinate 0.5
Stearyl glycyrrhizinate 0.05
Isopropylmethylphenol 0.1
Align In 0.1
D-pantothenyl alcohol 0.1
Salicylic acid 0.5
Urea 5.0
l-menthol 0.9
dl-menthol 0.2
1,3-butylene glycol 5.0
Sodium citrate 0.2
Methylparaben 0.1
Hinokitiol 0.003
Photosensor No. 201 0.002
Purified water (enough to make the total volume 100 parts)

Formulation example 2. Lotion [Ingredients] Parts Glyceryl caprylate 3.0
Polyglyceryl-10 Laurate 3.0
Cetanol 2.0
Behenyl alcohol 2.0
Methylparaben 0.1
Compound of Preparation Example 1 0.1
Yeast hydrolyzate 2.0
Ascorbic acid 3.0
Glycyrrhizic acid 0.5
β-glycyrrhetinic acid 0.05
Tocopherol nicotinate 0.1
Resorcinol 0.1
Zinc oxide 2.0
dl-camphor 0.5
Glycerin 2.0
1,3-butylene glycol 5.0
Potassium hydroxide 0.5
Purified water (enough to make the total volume 100 parts)

Formulation Example 3. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of rice extract hydrolysate was used in place of the yeast hydrolysate contained in Formulation Example 2.

Formulation Example 4. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of rice bran extract hydrolysate was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of fermented Job's tears seeds.

Formulation Example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of sake lees extract derived from sake was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 7. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of Benifuuki tea extract.

Formulation Example 8. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of rosa damask flower extract.

Formulation Example 9. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of cherry blossom extract.

Formulation Example 10. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of black soybean hydrolysate was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 11. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of red clover root extract was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 12. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of chamomile extract was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 13. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of gentian extract.

Formulation Example 14. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of perilla leaf extract was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 15. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that the yeast hydrolysate of Production Example 1 contained in Formulation Example 2 was replaced with 2.0 parts of lotus seed extract.

Formulation Example 16. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of lotus seed fermentation product was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Formulation Example 17. Lotion A lotion was obtained in the same manner as in Formulation Example 2, except that 2.0 parts of Morus alba extract was used in place of the yeast hydrolysate of Production Example 1 contained in Formulation Example 2.

Prescription Example 18. Lotion [Ingredients] Parts Jojoba oil 1.0
Polyoxyethylene (5.5) Cetyl Alcohol 5.0
Methylparaben 0.1
Compound according to Production Example 1 0.2
Ascorbic acid glucoside 2.0
Tranexamic acid 2.0
ε-aminocaproic acid 0.1
Sulfur 0.2
Estradiol 0.1
Glycerin 5.0
1,3-butylene glycol 5.0
Sodium citrate 0.2
Sodium metabisulfite 0.2
d-camphor 0.1
Purified water (enough to make the total volume 100 parts)

Formulation example 19. Emulsion [Ingredients] Parts Squalane 5.0
Cyclopentanesiloxane 1.0
Hexalan 3.0
Hexyldecyl isostearate 1.0
Caprylic/Capric Triglyceride 1.0
Polyglyceryl-10 Laurate 5.0
Polyglyceryl-10 Isostearate 5.0
Ascorbyl dipalmitate 15.0
Hydrogenated soy lecithin 1.5
Compound according to Production Example 1 2.0
Ascorbic acid phosphate magnesium salt 3.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Xanthan gum 0.2
Tremella fuciformis polysaccharide 0.2
Sodium hyaluronate 0.01
Tocopherol acetate 0.3
Tocopherol nicotinate 0.1
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Isopropylmethylphenol 0.1
Water-soluble collagen 1.0
Hydrolyzed Collagen 1.0
Sodium hyaluronate 1.0
Purified water (enough to make the total volume 100 parts)

Formulation Example 20. Milky Lotion A milky lotion was obtained in the same manner as in Formulation Example 19, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

Formulation Example 21. Milky Lotion An emulsion was obtained in the same manner as in Formulation Example 19, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide.

Formulation Example 22. Milky Lotion An emulsion was obtained in the same manner as in Formulation Example 19, except that 2.0 parts of ascorbic acid phosphate magnesium salt was replaced with 3.0 parts of 3-O-ethyl ascorbic acid.

Prescription Example 23. Cream [Ingredients] Parts Olive oil 5.0
Jojoba oil 5.0
Squalane 5.0
Hexyldecyl isostearate 5.0
Di(octyldodecyl/phytosteryl/behenyl) lauroyl glutamate 5.0
Glyceryl caprylate 1.0
Glyceryl stearate 1.0
Isostearyl glyceryl 3.0
γ-oryzanol 0.1
Behenyl alcohol 2.0
Palmitic acid 2.5
D-pantothenyl alcohol 3.0
Allantoin 0.1
Riboflavin 0.01
Resorcinol 0.1
Benzalkonium chloride 0.05
Urea 3.0
β-glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Ammonium glycyrrhizinate 0.1
Compound according to Production Example 1 3.0
Lactic acid bacteria fermented rice 2.0
Hydrogenated lecithin 0.5
Hydrogenated lysolecithin 0.5
Oil-soluble Panax ginseng extract 2.0
Xanthan gum 1.0
Zinc oxide 0.5
dl-camphor 0.3
l-menthol 0.5
Purified water (enough to make the total volume 100 parts)

Example 24. Pack [Ingredients] Parts Dipropylene glycol 5.0
Polyoxyethylene (60) hydrogenated castor oil 5.0
Cetyl alcohol 3.0
Behenyl alcohol 3.0
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Ammonium glycyrrhizinate 0.1
β-glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.1
Tocopherol acetate 0.5
Tocopherol nicotinate 0.1
D-pantothenyl alcohol 0.3
Resorcinol 0.1
Sulfur 2.0
Estradiol 0.002
Compound according to Production Example 1 0.2
Xanthan gum 2.0
Polyglyceryl-6 myristate 1.0
Potassium cocoyl glutamate 1.0
Hydrogenated lecithin 3.0
Hydroxylated lecithin 3.0
Purified water (enough to make the total volume 100 parts)

Formulation Example 25. Hair Shampoo [Ingredients] Parts Sodium laureth sulfate 10.0
Glyceryl monostearate 1.0
Coconut oil fatty acid diethanolamide 2.0
Polyoxyethylene (40) hydrogenated castor oil 0.5
Benzalkonium chloride 1.0
Stearyl alcohol 2.0
Behenyl alcohol 2.0
Dimethicone 3.0
Compound according to Production Example 1 0.2
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sodium salicylate 0.1
Tocopherol acetate 0.1
Pyrithione zinc 0.3
Benzoic acid 0.2
Triclosan 0.2
Citric acid 0.1
Propylene glycol 2.0
Purified water (enough to make the total volume 100 parts)

Example 26. Hair conditioner [Ingredients] Parts Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Benzalkonium chloride 1.0
Cetyl alcohol 3.0
Stearyl alcohol 1.0
Compound according to Production Example 1 0.2
Salicornia extract 1.0
Black soybean hydrolysate 1.0
Allantoin 0.1
Isopropylmethylphenol 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sulfur 0.5
Alkylisoquinolinium bromide solution (75%) 0.06
Pyrithione zinc 0.3
Methylparaben 0.1
Triclosan 0.2
Resorcinol 0.1
Purified water (enough to make the total volume 100 parts)

Formulation Example 27. Cleansing cosmetic [Ingredients] Parts Potassium cocoyl glycinate 5.0
Glycerin 10.0
Glyceryl caprylate 1.0
Sodium lauroyl aspartate 10.0
Compound according to Production Example 1 0.2
Cetyl alcohol 3.0
Myristyl alcohol 3.0
Isopropyl methyl alcohol 0.1
Allantoin 0.1
Sulfur 0.5
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Monoammonium glycyrrhizinate 0.1
β-glycyrrhetinic acid 0.05
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.2
Tocopherol acetate 0.2
Triclosan 0.1
Trichlorocarbanide 0.5
Trichlorohydroxydiphenyl ether 0.2
Concentrated benzalkonium chloride solution 50 0.2
Benzalkonium chloride 0.1
Purified water (enough to make the total volume 100 parts)

Prescription Example 28
A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients.
[Components] Parts Compound according to Production Example 1 1.0
Glycerin 3.0
1,3-butylene glycol 2.0
L-Ascorbic acid 2-glucoside 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Xanthan gum 1.0
Water-soluble collagen 1.0
Sodium hyaluronate 1.0
Eelgrass extract 1.0
Rice extract hydrolyzate 1.0
Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

Prescription Example 29. Beauty serum [Ingredients] Parts Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid hydrolysate 1.0
Fermented lotus seeds 1.0
Lactic acid bacteria culture 1.0
Compound according to Production Example 1 0.2
Citric acid 0.3
Sodium citrate 0.6
Purified water (enough to make the total volume 100 parts)

Claims (3)

A topical fat accumulation inhibitor for skin application containing vitamin B6 (desalinated pyridoxine hydrochloride) as an active ingredient. An intracellular active oxygen production inhibitor for topical use on the skin, containing vitamin B6 (desalinated pyridoxine hydrochloride) as its active ingredient. A topical prostaglandin E2 synthesis inhibitor for skin application containing vitamin B6 (desalinated pyridoxine hydrochloride) as the active ingredient.