JPS5923284B2 - Fruit fly attractant - Google Patents

Description

[Detailed Description of the Invention] This invention is based on acus cucurbita.
This invention relates to an attractant for eCoquil let.

The melon fly uses bitter gourd, kirari, loofah, watermelon, toga, kapocha, etc. as its main cultivated hosts, and lays eggs mainly in young fruits, shoots, and young stems by making oviposition holes, and the number of eggs laid is over 1,000 per generation. It is said to be an important pest with a high reproductive capacity, and is distributed in Southeast Asia, parts of Africa, the Hawaiian Islands, Oceania, Micronesia, etc.

The larvae feed on young fruits, reducing the fruit's commercial value or causing the crop to wither.

In Japan, it is said that parts of the Okinawa and Kagoshima areas are already contaminated with the melon fly, and eradicating it is a serious issue.

Methods for controlling melon flies include attractants such as cue.
-1ure or protein decomposition product mixed with insecticide to attract and kill (
Control methods include using a combination of methods such as attracting and killing the plant, removing wild hosts, burning damaged and unused fruit, or burying them in the soil.

By the way, cue-1ure has the disadvantage of sexual selectivity in that it attracts only male melon fruit flies, and is not acceptable in practice, and on the other hand, the proteolytic product has a unique unpleasant odor.

The present inventors have recently learned that a specific organic solvent extract of bitter melon has a strong effect of attracting the melon fruit fly.

The present invention relates to a medicinal fruit fly attractant characterized by containing as an active ingredient an extract obtained by extracting and concentrating a medicinal fruit fly attracting substance contained in bitter gourd with ether, methyl acetate, or ethyl acetate.

The attractant according to the present invention has no sex selectivity and attracts both male and female fruit flies, and does not emit any unpleasant odor like protein decomposition products.

The medicinal fly-attracting substance contained in bitter melon can be selectively extracted with ether, methyl acetate, or ethyl acetate.

To extract the medicinal fruit fly-attracting active substance with ether, methyl acetate, or ethyl acetate, grind the bitter melon fruit, add an appropriate amount of water and the above solvent, centrifuge, and concentrate the supernatant according to a conventional method. good.

The extract may be used as it is or after being diluted or concentrated if necessary, and placed in an insect trap such as a trap or trap, or may be adsorbed onto a suitable adsorbent material such as a TEX plate.

It is also common to mix insecticides to create an attractant.
All fall within the scope of the present invention.

Example l Crush 333g of bitter melon raw fruit with a mixer to make 330ru
of water and about 500 WLl of solvent are added and subjected to centrifugal extraction, and the supernatant is dehydrated and concentrated to about 50Ll using a 10 (m) Widmer precision instrument.

2 ml of this extract was added to 20 rnl of water, and its attractiveness to IJ fruit flies was assayed using vanetribin.

The results are shown in Table 1 below.

Example 2 4 rnl of attractant was added to 40 ru of water, and the number of insects caught was investigated using a glass trap.

The results are shown in Table 2 below.

Example 3 The ether extract obtained in Example 1 was diluted with pentane-ether (
6:4) solvent and pentane-ethyl acetate (85:15)
Thin layer chromatography was performed using a solvent, and the Rf value, color tone, and attractiveness of each fraction of the developed product were examined.

(1) The results of pentane-ether expansion are shown in Table 3 below.

(2) The results of pentane-ethyl acetate expansion are shown in the 4th section below.
Shown in the table.

As is clear from the above, in the present invention, not only can ether, methyl acetate, and ethyl acetate extracts be used as they are as active ingredients of attractants, but also the extracts can be further purified by an appropriate method and fractionated with high activity. may be collected and used as the active ingredient of an attractant, both of which are within the scope of the present invention.

For example, the ether extract may be further combined with pentane-ether (6:
According to thin layer chromatography using 4) and pentane-ethyl acetate (85:15) as the developing solvent, the developed product was 6
When fractionated, it appears that highly active substances exist in fractions with small R and f values.

However, in pentane-ether, R, fo is 75-0.
The presence of an active substance was observed in 50, whereas the R, fO, 78 to 0.62 fraction with pentane-ethyl acetate had activity but not to the same extent.

Claims (1)

[Claims] 1. A melon fly attractant characterized by containing as an active ingredient an extract obtained by extracting and concentrating a melon fly attracting substance contained in bitter gourd with ether, methyl acetate, or ethyl acetate.