JP2520084B2 - Attractants for the bean bug, Stinkbug and the stink bug - Google Patents

Description

Detailed Description of the Invention

[0001]

[Industrial applications]

[0002] The present invention relates to a hemiptera (Hipoptera, stink bug) belonging to the family Hemiptera Helicopteridae
atus) and Hymenoptera (Epidoptera: Psyllidae) (scientific name: Oencyrtus neza
rae) regarding the attractant, and in particular, in addition to the beetle bug, it also has an attracting effect on the beetle, the beetle, the natural enemy of the beetle bug,
In particular, the present invention relates to an attractant effective for controlling the bug bug.

[0003]

[Prior art]

Adult worms belonging to the family Hemiptera Helicopteridae occur in all parts of East Asia including Japan and cause great damage to soybeans and beans.

[0005] It is very difficult to prevent or control the damage caused by this bug, the conventional insecticide, and from the present situation, the occurrence of the insect pest in order to effectively control this pest. There is a strong demand for a predictive means for accurately knowing and a new type of control means different from conventional insecticides.

On the other hand, the chemical structures of sex pheromones and aggregate pheromones have been clarified so far for many pests.

Further, depending on the kind of the pest, it becomes possible to efficiently investigate the developmental fate of these insects by using these pheromone agents, and further, by using these attractants, a large amount of adult insects can be caught or males and females of both sexes can be caught. Methods for controlling pests by disturbing mating behavior are also being developed.

[0008]

[Problems to be Solved by the Invention]

[0009] However, in addition to having an attracting effect on the leaf beetle, it also has an effect of attracting the stink bug, which is the natural enemy of the beetle, and is particularly effective for controlling the leaf beetle. The drug is unknown.

[0010] Therefore, in addition to having an attracting effect on the leaf beetle, it also has an effect of attracting the stink bug, which is the natural enemy of the leaf beetle, and is particularly excellent in controlling the leaf beetle. An attractant was desired.

[0011]

[Means for Solving the Problems]

The present inventors have made such a background,
We conducted research on the assembly pheromone of the beetle, the beetle, and extracted a component that attracted male and female adult and second-instar larvae from the adult beetle, elucidated the chemical structure of the active substance, and found its chemical structure. It is known that the compound synthesized by the method has an effective attracting action to adult male and female second-instar larvae of the beetle bug, and at the same time, it is known to spawn on the eggs of the beetle bug, which is the largest of the beetle bugs. It was found that it also has an effective attracting effect on the natural enemy, the beetle Bombus terrestris.
The present invention has been completed.

That is, the means for solving the problems of the present invention are as follows.

First, (E) -2-hexenyl (Z)-
3-hexenoate, (E) -2-hexenyl (E)-
2-hexenoate, myristyl isobutyrate,
An attractant for P. stink bugs and stink bugs, which is contained as an active ingredient.

Second, (E) -2-hexenyl (Z)-
3-hexenoate (designated as E2HZ3H), (E)-
2-hexenyl (E) -2-hexenoate (E2HE
2H), myristyl isobutyrate (MI) and E2HZ3H: E2HE2H: MI = 0.5-
An attractant for the worm, Stinkbug and stink bug, containing a compound prepared to have a ratio of 1.5: 4.0 to 6.0: 0.5 to 1.5.

Third, (E) -2-hexenyl (Z)-
3-hexenoate, (E) -2-hexenyl (E)-
A compound containing 2-hexenoate, myristyl isobutyrate as an active ingredient was loaded on a device,
An attractant for the bean bug, Stinkbug and the stink bug.

Fourth, (E) -2-hexenyl (Z)-
3-hexenoate (designated as E2HZ3H), (E)-
2-hexenyl (E) -2-hexenoate (E2HE
2H), myristyl isobutyrate (MI) and E2HZ3H: E2HE2H: MI = 0.5-
An attractant for Betula chinensis and Buprestidae, in which a compound prepared to have a ratio of 1.5: 4.0 to 6.0: 0.5 to 1.5 is carried by a device.

Regarding the above second and fourth, E2HZ
The mixing ratio of 3H, E2HE2H, and MI is E2HZ3.
H: E2HE2H: MI = 0.5 to 1.5: 4.0
6.0: 0.5-1.5, preferably E2HZ3
H: E2HE2H: MI = 1: 5: 1.

(E) -2-Hexenyl [Hexenyl] (Z) -3, which is the active ingredient of the attractant according to the present invention.
-Hexenoate, (E) -2
-Hexenyl (E) -2-hexenoate, myristyl isobutyrate [Myristyl isobuty
rate] shows a strong attracting action and behavioral stimulating action on adult male and female larvae and second-instar larvae of P. stink bug.

Further, (E) -2-hexenyl (Z) -3-hexenoate, (E) -2-hexenyl (E) -2-hexenoate, myristyl isobutyrate, which are active ingredients of the attractant according to the present invention, are It also shows a strong attraction and behavioral stimulatory effect on the bug, Buprestidae.

To prepare the attractant according to the present invention,
(E) -2-hexenyl (Z) -3-hexenoate,
(E) -2-hexenyl (E) -2-hexenoate,
It can be carried out using myristyl isobutyrate by utilizing the formulation technology usually applied in the preparation of sex attractant.

For example, (E) -2-hexenyl (Z)-
3-hexenoate, (E) -2-hexenyl (E)-
2-hexenoate, myristyl isobutyrate,
As it is or as a solution using an organic solvent such as dichloromethane, the solution is adsorbed to an appropriate device, for example, plastic such as polyethylene or rubber, or a plastic capsule or an appropriate material or device according to the capillary mode. Can be used as an attractant in a practical form.

[0023]

Embodiment 1

An example of the attractant according to the present invention will be described below.

(E) -2-Hexenyl (Z) -3-hexenoate (designated as E2HZ3H), (E) -2-hexenyl (E) -2-hexenoate (designated as E2HE2H), myristyl isobutyrate (designated as MI) To
The attractant 1 according to the present invention was prepared by setting E2HZ3H: E2HE2H: MI = 1: 5: 1.

[0026]

Example 2

As in Example 1, (E) -2-hexenyl (Z) -3-hexenoate (designated as E2HZ3H), (E) -2-hexenyl (E) -2-hexenoate (designated as E2HE2H), Myristyl isobutyrate (denoted as MI) was added to E2HZ3H: E2HE2H:
Compounds were prepared by making MI = 1: 5: 1.

The attractant 2 according to the present invention was prepared by impregnating 100 mg of this compound into a device of pellets composed of an ethylene-vinyl acetate copolymer.

[0029]

Example 3

As in Example 1, (E) -2-hexenyl (Z) -3-hexenoate (designated as E2HZ3H), (E) -2-hexenyl (E) -2-hexenoate (designated as E2HE2H), Myristyl isobutyrate (denoted as MI) was added to E2HZ3H: E2HE2H:
The compounds were prepared so that MI = 1: 5: 1.

An attractant 3 according to the present invention was prepared by impregnating 100 mg of this compound into a device composed of ES fiber and polyethylene film.

[0032]

[Test Example 1]

In order to identify the attractant component substances, the component substances were traced by a method of detecting the change in nerve potential generated when the antennae received the active substance, using a bioelectric detector directly connected to the gas chromatograph.

After the field collection, male adults of the beetle bug, which was bred in a soybean pod, were housed in a glass container, and the air around the male adults was supplied with porous air while supplying clean air. The column packed with Tenax TA) was run for 1 day.

Immediately thereafter, the extract was obtained by passing hexane through the Tenax column.

A gas chromatographic analysis of this extract revealed a large number of components.

Then, when the active component was examined by using the gas chromatography / direct contact angle potential detector method (GC-EAD), four EADs were obtained as shown in FIG.
An active peak was observed.

These four components are HP-1 and DB-wa.
When injected into a gas chromatograph direct coupling mass spectrometer (GC-MS analysis) using the x column and analyzed, as shown in FIG. 1, (E) -2-hexenyl hexenoate: peak I, (E), respectively. -2-hexenyl (Z)
-3-Hexenoate: peak II, (E) -2-hexenyl (E) -2-hexenoate: peak III, myristyl isobutyrate: peak IV were identified.

Of these, it was found that peak I is an alarm pheromone secreted from adult male and female worms of the Japanese bug bug, and the remaining three components (peaks II, III, IV) are aggregate pheromones.

The separately synthesized (E) -2-hexenyl (Z) -3-hexenoate, (E) -2-hexenyl (E) -2-hexenoate and myristyl isobutyrate were compared with each other and found to be consistent with the active natural product. .

From the above results, a substance (natural product) having an extremely high attracting action to the Helicopteridae, which belongs to the family Hemiptera Helicoptera, is (E) -2-hexenyl (Z) -3-hexenoate, It was determined to be (E) -2-hexenyl (E) -2-hexenoate, myristyl isobutyrate.

(E) -2 obtained by chemical synthesis
-Hexenyl (Z) -3-hexenoate, (E) -2
When -hexenyl (E) -2-hexenoate, myristyl isobutyrate was subjected to gas chromatography / direct contact angle potential detector method (GC-EAD), a clear EAD response could be recorded.

[0043]

[Test Example 2]

As a group of the present invention, ten plastic containers containing the attractant 2 obtained in Example 2 above were prepared.

Further, as a control group 1, a plastic container in which male adults of the beetle beetle (10) were put together with soybeans and water, and as a control group 2, an empty plastic container in which nothing was put. 10 were prepared for each.

Each of the 10 plastic containers in each section prepared above was set in a hand-made basin type insect trap,
Ten traps were prepared for each ward, each trap was left in a soybean field in Kumamoto Prefecture, and an insect trap test was conducted under the same environmental conditions.

The results of the test are as follows.

In the present invention section, the total of 10 traps is
100 second-instar larvae of the leaf-bellied stink bug, 90 female adults of the giant beetle, 1 male adult beetle
I was able to catch 20 insects.

Control section 1 is a total of 10 traps,
70 second-instar larvae of Hoso-helika stink bug, 128 female adults of Hoso-helika stink bug, 1 male adult of Hoso-helika stink bug
I was able to catch 10 insects.

Control area 2 is a total of 10 traps,
0 second-instar larvae of the beetle bug, 0 female imago of the beetle bug, and 0 male imago of the beetle bug, that is, none of them could be caught.

As shown in the above results, the present invention group, like the control group 1, showed a strong attracting force to the beetle bug.

[0052]

[Test Example 3]

As the group of the present invention, the attractant 1 obtained in Example 1
Ten cylindrical sticky insect traps equipped with (50 mg) were prepared.

The cylindrical sticky insect trap is devised so as not to trap the worm bug.

As control section 1, 10 sticky insect traps similar to those of the present invention section without attractant were prepared, and as control section 2, adhesive suction by Tokyo A & S Co. without attractant was prepared. Ten insect traps were prepared.

Each insect trap was installed in a soybean field in Kumamoto prefecture in 1993.
Left for 28 days from September 21st to October 17th,
Under the same environmental conditions, the insect trap test of the bug, Buprestidae, was performed to determine the average number of trapped insects per trap.

The results of the test are as follows.

In the present invention group, 18 female adults of P. mellifera and 1 male adult of P. mellifera could be caught.

In the control group 1, one female imago of the bug beetle, and 0 male imago of the bug beetle.
I was able to catch some insects.

In the control group 2, 3 female adults of the bug beetle and 5 male adults of the bug beetle are included.
I was able to catch some insects.

As shown in the above results, in Control Group 2, no significant difference was observed between the female and the male in the trapped bug, Bombus terrestris.

On the other hand, in the present invention group, overwhelmingly many females were captured.

When the results of Test Example 2 and Test Example 3 described above are considered together, the attractant according to the present invention not only exhibits an attracting effect on the helicopter beetle belonging to the hemiptera Helicopteridae, It was confirmed that it has a very high attracting effect on the females of the parasitoid wasps belonging to the family Hymenoptera, Vespididae, which is the natural enemy of Parasitidae (parasitizing eggs).

[0064]

【The invention's effect】

According to the attractant of the present invention, in addition to having an attracting effect on the leaf beetle, it also has an effect on the stink bug, which is the natural enemy of the beetle bug, so that the beetle, Beetle bug It has an excellent control effect.

[Brief description of drawings]

FIG. 1 is a graph showing the results of analysis by gas chromatography.

Claims (4)

(57) [Claims] 1. A beetle and stink bug, which contains (E) -2-hexenyl (Z) -3-hexenoate, (E) -2-hexenyl (E) -2-hexenoate, myristyl isobutyrate as an active ingredient. An attractant to the bigeye bee. 2. (E) -2-Hexenyl (Z) -3-hexenoate (designated as E2HZ3H), (E) -2-hexenyl (E) -2-hexenoate (designated as E2HE2H), myristyl isobutyrate (MI and Do)
To E2HZ3H: E2HE2H: MI = 0.5 to 1.
5: 4.0-6.0: 0.5-1.5 attractant of the above-mentioned beetle bug, Stinkbug, and a bumblebee, containing the compound prepared so that it might become. 3. A device carrying a compound containing (E) -2-hexenyl (Z) -3-hexenoate, (E) -2-hexenyl (E) -2-hexenoate, myristyl isobutyrate as an active ingredient. , An attractant for the bug bug, Stinkbug, and the bug. 4. (E) -2-Hexenyl (Z) -3-hexenoate (designated as E2HZ3H), (E) -2-hexenyl (E) -2-hexenoate (designated as E2HE2H), myristyl isobutyrate (MI and Do)
To E2HZ3H: E2HE2H: MI = 0.5 to 1.
An attractant for Betula chinensis and Buprestidae, in which a compound prepared to be 5: 4.0 to 6.0: 0.5 to 1.5 was carried by a device.