JPS5940128B2 - Koushiyokubutsuvirus Seikakubunnoseiho - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an excellent anti-plant virus fraction that can be put to practical use.
A yeast cell extract obtained by alkali extraction for a short time under conditions of H8 to PH12 and temperature of 70°C to 100°C is adjusted to PH1.6 to PH4.6, and the resulting precipitate is removed to obtain a supernatant. This invention relates to a method for producing an anti-plant virus fraction.

In recent years, with the development of the microbial industry, microbial cells, which can be called a treasure trove of useful substances, have become available stably in large quantities and at relatively low prices.

Humans have been using microorganisms for a long time and have received great benefits from them, but we are still only using a portion of the capabilities of microorganisms, and it is hoped that they can be used even more effectively. Especially in these days when there is a call for the effective use of unused resources, there is a strong desire in the microbial industry to effectively utilize the abilities of microorganisms in a wide variety of fields, including food, medicine, industry, and agriculture. In this situation, damage to crops caused by plant viruses is extremely large, resulting in large industrial losses. On the other hand, yeast cell extract (Japanese Patent Publication No. 49-20490) and agricultural antiviral agent (Japanese Patent Application Laid-open No. 1983-20490) are considered safe anti-plant virus agents.
Although yeast-based materials such as 0-12230) have been proposed, they have not yet been put to practical use. Therefore,
The present inventors believe that anti-plant virus agents that can be put to practical use include:
From the viewpoint that it is necessary to satisfy the requirements that it is safe, effectively prevents virus infection, has so-called systemic transfer properties that suppress the proliferation of viruses in the plant body, and can be manufactured economically. As a result of intensive research into a method for producing a practically usable anti-plant virus fraction from the unused parts of yeast, we found a supernatant obtained by acidifying the extract extracted from yeast cells with an alkaline aqueous solution and removing the resulting precipitate. But, high~・
They discovered that it exhibits anti-plant virus properties and completed the present invention.

The yeast cells used in the present invention may be ordinary yeast cells obtained by culturing by ordinary methods, and are not particularly limited.

In the present invention, alkaline extraction is performed from yeast cells, and the conditions are PH8 to PH12, heating at a temperature of 70°C to 100°C, and short-time extraction within 10 minutes to lower the pH. Immediately separates and removes insoluble matter without adjustment.

According to the method of the present invention, it is possible to extract in high yield while minimizing the molecular weight reduction of the extract. If extracted under conditions other than the extraction conditions of the present invention, the yield may be low, causing economical problems, or the molecular weight may be reduced, leading to a decrease in anti-plant virus properties. In the present invention, the extract thus obtained is used as PHL. Adjust the pH to 6 to 4.6.

The precipitate produced at this time is mainly composed of nucleic acids and others, and can be used as a raw material for seasonings, etc. Conventionally, the denucleated soil obtained by separating and removing this precipitate was simply discarded, but the present inventors discovered that the supernatant exhibits high anti-plant virus properties. That is, on the enucleation obtained by the present invention? exhibits high anti-plant virus properties even when neutralized or desalted using conventional methods. Of course, the active ingredient can also be further purified by dialysis, electrodialysis, gel filtration, ion exchange, organic solvent precipitation or insoluble salt formation, adsorption, and other methods. If the method of the present invention is followed, it is possible to provide an economical and practically usable anti-plant virus agent that has excellent virus infection inhibiting ability and systemic transferability from the unused portion of yeast that was previously discarded. Its value is extremely great.

The present invention will be explained below with reference to Examples, Comparative Examples, and Test Examples.

Examples 1 to 3; Comparative Example Yeast milk of Candida utilis with a moisture content of 88% was heated to 80'C, and caustic soda was added to produce MimiPHlO.
The mixture was heated and extracted for 3 minutes.

Twice the amount of water was added to this, and the mixture was immediately centrifuged to remove insoluble materials to obtain a yellow extract (Comparative Example 1). The extract was heated to 7°C.
After cooling, add sulfuric acid to pH 2, centrifuge the resulting precipitate, and remove the nuclei. (Example 1) was obtained. In addition, the denucleated soil was dialyzed against running water for 2 days using a Visking tube to separate the residual fraction (Example 2) and the strongly basic ion exchange resin 1R-
118 and a strongly acidic ion exchange resin 1R-410 (Example 3) was obtained. As test specimens, seedlings of Nicotiana glutinosa with three leaves, the upper leaf, the middle leaf, and the lower leaf, centered on the largest leaf at the 14th to 15th leaf stage, were used. A 0.5% aqueous solution of the drug and water for the control plot were sprayed only on the front and back of the middle leaves, and after drying, tobacco mosaic virus was inoculated on the top and bottom of three leaves according to the conventional carborundum method.

Claims (1)

[Claims] 1. The yeast cell extract obtained by alkali extraction from yeast cells in a short time under conditions of PH8 to PH12 and temperature of 70°C to 100°C is adjusted to PH1.6 to PH4.6 to remove the precipitated fraction. A method for producing an anti-plant virus fraction, which comprises obtaining a supernatant.