JPS5946209B2 - Livestock drugs - Google Patents

Description

[Detailed description of the invention] FIELD OF THE INVENTION The present invention relates to pharmaceuticals for livestock.

More specifically, the present invention relates to a drug for livestock containing Lactobacillus salivarius.

Previously, it was known that L(t)lactic acid has a useful physiological effect on livestock, and that administering lactic acid bacteria to livestock is useful for gastrointestinal disorders in livestock.
...Currently, there are no lactic acid bacteria that have a high ability to produce lactic acid and are satisfactory as livestock drugs.

In view of this situation, the present inventors have conducted intensive research and found that Lactobacillus salivarius has an extremely high L(-1
-) It not only shows the ability to produce lactic acid, but also has good colonization in the gastrointestinal tract of livestock, especially artiodactyls, especially pigs. The present inventors have completed the present invention by discovering that the present invention has a preventive and therapeutic effect on diseases, an effect on improving feed conversion ratio, an effect on deodorizing feces, etc.

Lactobacillus salivarius used in the present invention may be any species as long as it colonizes the digestive tract of the intended livestock.

Such colonization can be determined, for example, by administering a certain number of Lactobacillus salivarius to intended livestock for a certain period of time, and determining the number of inoculated bacteria detected in feces or the period during which inoculation is observed in feces. For example, if 106/kg of bacteria is administered to the intended livestock for 5 days, 106/kg or more of the bacteria is detected in the feces, or even if the inoculation is continued for 5 or more days, particularly for 7 or more days after administration is stopped. Those that can be detected are preferred.

Among these bacteria, the mycological properties of preferable bacteria are as listed in the table below, and examples of such preferable bacteria include, for example, Lactobacillus salivarius 135-BT.
(National Institute of Microbial Technology, Agency of Industrial Science and Technology, Microbiology Accession Number F
ERM-PA2445).

For culturing Lactobacillus salivarius, both solid and liquid media can be used.

Further, as a culture method, any of the static culture method, shaking culture method, deep culture method, etc. can be used, but the static culture method or the deep culture method in a liquid medium is particularly preferable.

As for the medium composition, one containing a carbon source, nitrogen source, etc. that can be assimilated by the bacteria is used.

As the carbon source, for example, assimilable sugars such as glucose, sucrose, and maltose are used, and as the nitrogen source, for example, peptone, corn steep liquor, meat extract, and other organic nitrogen-containing substances are used.

Other small amounts of inorganic salts, such as common salt, phosphates, metal salts such as calcium, manganese, and iron, and micronutrients such as various vitamins, may be added as appropriate. A surfactant such as Span may also be added.

The pH of the medium is pH 4.0 to 7.8, especially pH 6.5.
The culture temperature is preferably maintained at 20-45°C, particularly around 37°C.

Lactobacillus salivarius used in the present invention may be in any state as long as it is a living organism, such as cultured cells, freeze-dried products thereof, and spray-dried products thereof.

Lactobacillus salivarius, which is the active ingredient of the present invention, may be administered directly to livestock, or may be formulated into powders, tablets, granules, pellets, etc. by known means for producing lactic acid bacteria preparations, and administered. It may also be administered by adding it to feed, drinking water, etc.

In addition to Lactobacillus salivarius, the livestock drug of the present invention includes appropriate livestock drugs such as streptomycin and oxytetracycline.

Chlortetracycline, bacitracin, tylosin, destomycin, Haig's tyrosin, flagilidone, and the like may also be blended.

The number of Lactobacillus and Salivarius bacteria in a preparation is determined depending on the dosage form, type of strain, etc., but usually 1
It is preferably 104 or more, particularly 106 or more per g.

The number of doses of Lactobacillus salivarius to be administered to livestock is appropriately determined depending on the type of livestock, type of disease, symptoms, etc., but for the purpose of promoting the growth of pigs, for example, it is usually 105 -
It is advantageous to administer about 106/kg.

Example 1 Lactobacillus salivarius 135-BT was grown in a broth medium supplemented with 1% glucose and 0.1% Twin 80 at 37°C.
After culturing for 20 hours, the bacteria collected using a centrifuge were
% lysine, 0.05% cystine.

Add 0.05% ascorbic acid aqueous solution to 1rrLl
The number of bacteria was adjusted to about 30 billion, and a homogeneous bacterial suspension was produced using a blender.

Sterilized potato starch 20 while stirring this bacterial suspension 171
kg to make it homogeneous to prepare bacterial starch with a moisture content of 11-14%.

This bacterial starch was placed in a shallow container, placed in a multistage vacuum dryer, and dried at a vacuum of 1 mmHg for about 5 hours.

The thus obtained powder consists of 1% dried bacterial starch, 11% lactose, 8% calcium carbonate, 70% potato starch, and 10% bentonite.

Example-2 1% of the dried bacterial starch obtained in Example 1, 11% of lactose, 75% of potato starch, and 10% of carrion.

A granule prepared by adding water to a 3% CMC mixture, kneading it, and granulating it.

Example 3 Cultured and collected Lactobacillus sagitvarius 135-B
T cells were dispersed in a dispersion medium (gelatin 0.75%).

Sodium glutamate 0.1 M, sodium succinate 0.
(prepared by adding dextrin to 30% to a solution consisting of 1M) to prepare a bacterial suspension so that the number of bacteria is about 30 billion in 1ml, and this bacterial suspension Using a Mobil Miner type spray dryer (manufactured by 0), the rotation speed was 25,000 rpIn, the inlet temperature was 120°C, the outlet temperature was 60°C, and the dropping rate of the suspension was 2000771 Vh.
Spray drying was carried out under the conditions of r.

Tablets are made by compressing the thus obtained powder of 1% dry tooth powder, 50% lactose, 5% calcium carbonate, 40% potato starch, and 4% talc.

Reference Example 1 Starch was added to Lactobacillus sagitvarius 135-BT cells, vacuum freeze-dried, and starch was added again to obtain a dry powder with a viable cell count of 109 per gram.

A total of 18 Landrace pigs, 6 females and 12 males, aged 30 days, were randomly divided into 3 groups of 6 pigs each, and the first group received 0.6% of the bacterial dry powder obtained as above. With added feed,
The second group was fed a feed containing 0.06% of the bacteria dry powder, and the third group (control group) was fed a feed with no bacteria added, and the properties of their feces were observed for 14 days.

For the test, a commercially available pig artificial milk late stage feed was used, and ad libitum feeding,
The animals were kept in groups with constant water supply.

The results are shown in Table 2, and in the test group, no diarrhea congestion or new diarrhea was observed, and a clear effect of Lactobacillus salivarius administration was observed.

Reference Example 2 Starch was added to Lactobacillus sazivarius 135-BT cells, spray-dried, and starch was added again to obtain a dried bacterial powder with a viable cell count of 108 per gram.

2 Landrace sows with 4 litters weighing around 8kg.
After completing the complete castration and vaccination against classical swine fever and swine erysipelas, the animals were divided into two groups of 10 each, a test group and a control group. 0.
A 3% bacterial suspension a O suspension was constantly supplied as drinking water, and the control group was similarly supplied with a starch suspension containing no bacteria.

In addition, both groups were fed a commercially available pig artificial milk feed for the first half of the test for one week after the start of the test, and from the second week onwards, they were fed a commercially available pig artificial milk feed for the latter half.

The test was conducted for 5 weeks to examine the effect of Lactobacillus salivarius 135-BT on the growth of pigs.

The results are shown in Table 3, and in the test group, both weight gain and feed utilization were excellent, and a clear effect of Lactobacillus salivarius administration was observed.

Claims (1)

[Claims] 1. A drug for livestock containing Lactobacillus salivarius.