JPS6020992B2 - Method and device for sterilizing mushroom culture medium - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to a method for sterilizing a mushroom culture medium and its refilling,
More specifically, the culture medium is heated until the temperature rises to or near the boiling point and the culture medium reaches the required solubility;
Hereafter, we will introduce a method for sterilizing mushroom toji, which not only shortens the sterilization time and saves energy by sterilizing with ozone, but also enables good mycelial culture by converting the ozone absorbed into the culture medium into oxygen. Regarding equipment.

Conventionally, as a method for sterilizing mushroom culture media, normal pressure or high pressure steam sterilization is generally performed using a sterilization pot. However, such conventional sterilization methods require long heating time of about 5 to 6 hours in a normal pressure cooker and about 2 hours in a high pressure cooker, and have the drawback of consuming a large amount of fuel such as heavy oil.

In addition, the modern mushroom cutting industry has become an energy-consuming industry, with large amounts of electricity being consumed to move various equipment such as air-conditioning equipment, talking machines, and bacteria scrapers, which is putting pressure on management. There is. The inventor focused on the fact that a large amount of fuel is consumed especially in the sterilization process, and conducted various studies to improve the process.

In general, sterilization of mushroom towers is carried out not only to kill microorganisms that are mixed into the culture medium, but also to heat and dissolve the culture medium to make it easier for mycelia to absorb nutrients.

Furthermore, it is said that the formalin present in the sawdust has the effect of evaporating growth-inhibiting substances such as tannins. In conventional high-temperature steam sterilization, microorganisms cannot be completely killed unless the high temperature is maintained for a long period of time, as described above. However, the inventor discovered that when the temperature of the medium rose to near the boiling point, the powder was already sufficiently dissolved and various inhibitory substances were also sufficiently removed.
Furthermore, we have come up with the idea that various types of fungi that have not been killed up to this point can be killed in a short time by flowing ozone-containing air without using high-temperature steam. This method saves fuel and allows complete sterilization in a short period of time. A mushroom cultivation container filled with a culture medium and sealed with a stopper is placed in a steam sterilization pot, heated until the medium reaches the required solubility, and then heated. A method for sterilizing a mushroom culture medium characterized by stopping heating and then introducing a gas containing ozone into a sterilizing pot, a steam sterilizing pot 10 equipped with an exhaust valve 12, and a steam sterilizing figure 1. To provide an apparatus for sterilizing a mushroom culture medium, which is characterized in that it comprises an ozone generator 14 communicated through an ozone generator 18.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The inventor used the toughness value of the medium as a standard for the solubility of the medium.

In other words, as shown in Fig. 1, if we look at the changes in the sugar content of the medium and the changes in the culture medium temperature in relation to the heating time of the sterilization pot, we can see that by the time the culture medium temperature reaches approximately 100°C, the sugar content will be approximately 12.
The sugar content goes from 13 to 13, reaching the upper limit, and the sugar content hardly changes after that. Judging from the final yield obtained by carrying out the method of the present invention, even if heating is stopped when the temperature rises to the boiling point, when the bran content reaches 12 to 13, the medium has sufficient solubility. In addition, the growth-inhibiting substances in the medium have been sufficiently evaporated. In other words, heating after reaching the required sugar content as in the conventional method,
This means that fuel was consumed only for the purpose of sterilizing microorganisms.

An example of the sterilizer according to the present invention is shown in FIG.

In the figure, 1st floor is a normal pressure sterilizer, and 1st exhaust valve is shown.
It has 2. 14 is an ozone generator, and a connecting pipe 16
The lotus is suitable for inside the sterilization pot 10.

18 is a valve provided in the connecting pipe 16.

The ozone generator 14 may be either a discharge type or an ultraviolet irradiation type. To use the above-mentioned sterilizer, first fill the mushroom cultivation container with culture medium and put it in the sterilizer pot 1 (not shown).
Contain within 0.

A burner (not shown) in the sterilization pot 10 is ignited and heated, and heating is stopped when the temperature of the medium rises to the boiling point.

Next, the exhaust valve 12 is opened to exhaust the high temperature steam inside the pot. Next, the exhaust valve 12 is closed, and when the temperature inside the sterilization pot 10 has decreased to a serious level, the ozone generator 14 is activated and the valve 18 is opened.

Since the inside of the sterilizing pot 10 is cooled and has a negative pressure, when the outside air passes through the ozone generator 14, oxygen is converted to ozone, and this ozone-containing air is sent from the connecting pipe 16 to the sterilizing pot 10.
The inside of the pot and the culture medium are sterilized.

In this case, the ozone concentration in the flowing air is preferably around Pm. When the temperature inside the pot drops to approximately 70° C. or lower, the ozone generator 14 is stopped, and thereafter the pot is opened to cool down.

Although the air containing ozone was introduced into the pot by the suction effect of negative pressure as described above, the air containing ozone was forced to be fed by a fan (not shown) while the exhaust valve 12 was kept open. Good too.

Figure 3 shows the average culture medium temperature change when using a normal pressure cooker with 1,200 800cc bottles, and Figure 4 shows the average temperature change when using a conventional high-pressure cooker holding 1,800 800cc pins. Indicates soil temperature changes.

From Figure 3, position A, that is, 2 points from the start of heating.
The boiling point temperature is reached in 00 minutes.

In the case of the present invention, heating is stopped here. In the conventional case, since atmospheric pressure sterilization is used, it is necessary to continue heating for 4 hours from position A, which adds up to 440 ml from the start of heating.
minutes are required.

In other words, compared to the conventional method, the time can be reduced by 4 hours, and fuel can be saved by about 55%. Next, looking at Figure 4, the boiling point is reached in about 24 minutes.

In the conventional case, to reach a temperature of 120 qo at high pressure,
It is necessary to heat the product for an additional 60 minutes, and for sterilization, it is necessary to stop heating and maintain the high temperature for an additional 60 minutes. In other words, in the case of the method of the present invention, compared to conventional high-pressure sterilization, the time is reduced by 2 hours, and the fuel consumption is approximately 20%.
You can save money. Table 1 shows the average yield per bottle and the incidence of miscellaneous bacteria by the conventional sterilization method and by the sterilization according to the present invention.

Table 1 From Table 1, there is almost no difference in average yield and bacterial incidence between the conventional method and the method of the present invention.

As described above, the method and apparatus of the present invention not only have a sterilization effect that is equal to or greater than that of the conventional method, but also have the chopstick effect of significantly shortening the time required for sterilization and significantly reducing fuel consumption. play.

Furthermore, the introduced ozone is absorbed by the water in the culture medium and turns into oxygen in a relatively short time, increasing the oxygen content of the culture medium.
Various vitamins are not destroyed either, resulting in a state suitable for culturing mycelium. In addition, in the device, the simple structure of the conventional normal pressure sterilization pot is sufficient for the sterilization pot body, and the cost can be reduced and the sterilization effect can be more effective than that of the conventional high pressure sterilization pot. Although the present invention has been variously explained above with reference to preferred embodiments, the present invention is not limited to these embodiments, and it goes without saying that many modifications can be made without departing from the spirit of the invention. It is.

[Brief explanation of the drawing]

Figure 1 is a graph showing the relationship between heating time, culture medium temperature, and sugar content of the culture medium, Figure 2 is a schematic diagram of the device of the present invention, and Figure 3 is a graph showing the change in culture medium temperature when using a conventional high-pressure sterilization pot. The graph shown in FIG. 4 is a graph showing changes in culture medium temperature when a conventional atmospheric pressure sterilization pot is used. 10...Steam sterilization pot, 12...Exhaust valve, 14.
...Ozone generator, 16...connecting pipe, 18...bulf. Figure 1 Figure 2 Figure 3 Figure 4

Claims (1)

[Scope of Claims] 1. A mushroom cultivation container filled with a culture medium and sealed is placed in a steam sterilization pot, heated until the medium reaches the required solubility, then heating is stopped, and ozone is then introduced into the sterilization pot. A method for sterilizing a mushroom culture medium, the method comprising introducing a gas containing the mushrooms. 2. A mushroom culture sterilization device characterized by comprising a steam sterilization pot 10 equipped with an exhaust valve 12 and an ozone generator 14 communicating with the steam sterilization pot 10 via a valve 18.