JPS6041592B2 - mushroom culture medium - Google Patents

Description

[Detailed Description of the Invention] The present invention provides air permeability and nutrients such as cellulose, nitrogen, phosphorus, potassium, and trace elements suitable for promoting the growth of mushroom hyphae of wood-decaying fungi such as Nameko, Tamogitake, Shiitake, and Oyster mushrooms. The present invention relates to a rich and inexpensive mushroom culture medium.

Conventionally, as a culture medium for mushrooms, sawdust, which has a high C/N ratio, is mixed with 10-20% rice bran, which has a low C/N ratio, as a nutrient source, and this is adjusted to have a moisture content of 60-70%. , which has the disadvantage of poor air permeability and hinders the growth of mushroom hyphae.

For this reason, add 20 to 6% of the volume of rice hulls to the above culture medium,
There are examples of efforts to improve breathability. However, even though air permeability can be improved by mixing this rice husk, the C/N ratio of the rice husk itself is over 100, and the nutrients necessary for the growth of wood-decaying fungi such as oyster mushrooms, shiitake mushrooms, tamogitake mushrooms, and nameko mushrooms are not provided. As a result, the effect of improving the composition as a culture medium is small. In view of this, the present invention takes into consideration that the fungi of mushrooms such as Nameko, Oyster, Shiitake, and Tamogitake are aerobic bacteria, and that the C/N ratio suitable for mushroom growth is 3°C. This was developed to provide an inexpensive and high-quality mushroom culture medium with optimal nutrients for the growth of mushroom hyphae, and will be explained below based on examples.

Rice hull compost is produced by mixing rice hulls alone or in a mixture of sewage sludge and livestock waste collected from sewage treatment plants, etc. to adjust the moisture content, or by directly subjecting sewage sludge, etc. to aerobic fermentation.

The C/N ratio of this rice husk compost varies depending on the amount of rice husks mixed and the type of sewage sludge, but it is usually 20~
It will be 40. Growth of mushroom hyphae by using rice husk compost constructed as above alone or mixed with other culture medium (length of hyphae that spread and proliferated in the medium from the inoculated part)
We conducted a comparative experiment.

Example 1 As a culture medium, A total amount of sawdust, B sawdust: rice husk compost 2:1, C sawdust: rice husk compost 1:1, D sawdust: rice husk compost 1:2, E
Using 5 types of rice husk compost, the moisture content is 70.
% and inoculated with Nameko inoculum and Tamogitake inoculum,
The mushroom hyphae were cultured at 23°C and the speed of elongation was compared.

As a result, the results shown in FIG. 1 were obtained. That is, the growth was faster in the order of culture media E, D, C, B, and A. The mixing ratio of sewage digested sludge and rice husks in the rice husk compost used in this experiment was 2:3 by volume, and the C/N ratio after aerobic fermentation was
The ratio was 29.

Example 2 Culture media A″, B″, C″, D″, E″ were prepared by adding 10% rice bran to each of the five types of culture media in Example 1 above.
Nameko inoculum was inoculated into the cells and cultured in the same manner.

As shown in Fig. 2, the mycelium grew faster in the order of increasing rice husk compost mixing ratio, that is, in the order of E'', D'', C'', B'', and A''.Example 3 As a culture medium F sawdust: rice bran = 6:1, G sawdust: rice bran: rice husk = 4:1:2, H sawdust: rice bran: rice husk compost 4:1:2, I rice bran: rice husk compost 1:6 4 When Shiitake and Oyster mushroom seeds were inoculated at a moisture content of 60 to 65% and cultured at 23°C, the results shown in Figure 3 were obtained.

That is, the growth of hyphae was faster in the order of culture media 1, H, G, and F. Therefore, as can be seen from the above-mentioned Examples, it can be seen that a culture medium made of rice husk compost in its entirety or in a mixture can produce good results for the growth of mushroom hyphae. In other words, rice husk compost has a suitable air permeability for the growth of mushroom mycelia, and contains adequate amounts of nutrients such as nitrogen, phosphorus, and potassium.
When this culture medium is used, mycelia rapidly propagate inside the culture medium, and it is extremely advantageous for the production of wood-decaying fungus mushrooms such as Nameko, Somogitake, Oyster mushroom, and Shiitake. Due to its nature, it improves the aeration of the culture medium, and its constituent cellulose serves as a carbon source for the growth of mushrooms such as the above-mentioned Nameko, Oyster, Shiitake, and Tamogitake.On the other hand, sewage sludge is rich in nitrogen, phosphorus, potassium, etc. It has the advantage that the cellulose, lignin, etc. contained therein also serve as carbon sources, and the raw materials are inexpensive.

[Brief explanation of the drawing]

Figure 1a is an explanatory diagram showing the growth of Nameko mushrooms in different culture media, Figure 1b is an explanatory diagram showing the growth of Tamogitake mushrooms, and Figure 2
The figure is an explanatory diagram showing the growth of the nameko mushroom, and Figure 3 a is an explanatory diagram showing the elongation of the hyphae of the oyster mushroom, and Figure 3 b is an explanatory diagram showing the elongation of the hyphae of the shiitake mushroom.

Claims (1)

[Scope of Claims] 1 (1) A suitable amount of rice husk is mixed with sewage sludge, and the mixture is aerobically fermented to give a C/N ratio of 20 to 60, preferably 30. Rice husk compost is used as a culture medium component. A culture medium for mushrooms such as Nameko, Tamogitake, Oyster, and Shiitake, which is characterized by (2) Mix an appropriate amount of rice husks with sewage sludge, ferment this aerobically to make rice husks compost, and mix this with 1/2 or more volume of a culture medium containing sawdust and rice bran. Nameko mushrooms, Tamogitake mushrooms, Shiitake mushrooms, characterized by having a C/N ratio of 20 to 60, preferably about 30.
Culture medium for mushrooms such as oyster mushrooms. (3) Mix an appropriate amount of rice husk with sewage sludge and ferment it aerobically to produce rice husk compost, and mix this rice husk compost with more than 1/2 volume of sawdust to adjust the C/N ratio. A culture medium for mushrooms such as nameko, Tamogitake, shiitake, and oyster mushroom, characterized in that the culture medium has a concentration of 20 to 60, preferably about 30.