JPS6234011B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to dextranase-containing dentifrice compositions. BACKGROUND ART Conventionally, it has been proposed to incorporate dextranase into oral compositions such as toothpastes as an active ingredient for caries prevention in order to prevent the formation of dental plaque (Japanese Patent Publication No. 34897/1983). However, anionic active agents such as sodium lauryl sulfate are usually added to oral compositions, especially dentifrice compositions, in order to impart foaming properties and improve the feeling of use. There is a problem that dextranase is easily deactivated by anionic activators, and in particular, sodium lauryl sulfate, which has been widely used in the past, has a high degree of deactivation of dextranase. Among anionic activators, sodium lauroyl sarcosine is relatively effective in stabilizing dextranase, but sodium lauroyl sarcosine has problems such as peeling of the oral mucosa when added in large amounts. In this case, using a nonionic activator is effective for stabilizing dextranase, but the nonionic activator conventionally used for toothpaste compositions is inferior to anionic activators in terms of taste and foaming. Therefore, for dentifrice compositions that are required to have a good feel when used in the oral cavity, it is desirable to use an anionic activator from the viewpoint of the feel when using them. In view of the above circumstances, the present inventors have conducted extensive research with the aim of obtaining a toothpaste composition that is capable of stabilizing the combination of dextranase and is excellent in terms of usability, formulation stability, and safety for oral mucosa. As a result of summer,
In dextranase-containing toothpaste compositions, higher alcohol sulfate esters or their salts, such as sodium lauryl sulfate, which are known to easily deactivate dextranase, are added to N, such as sodium lauroyl sarcosinate.
- When used together with long-chain acylamino acids or their salts at a ratio of 1:2 to 2:1 (weight ratio) of the former, the deactivation of dextranase is surprisingly small, and it takes only one month at 40°C. We have discovered that it is possible to obtain a dentifrice composition in which dextranase is stably retained even when stored under harsh conditions, and which is also excellent in terms of usability such as foaming properties and formulation stability, and has developed the present invention. This is what led to the eggplant. The present invention will be explained in detail below. The present invention provides a dextranase-containing dentifrice composition in which dextranase is blended into a dentifrice composition such as a toothpaste, a moisturized toothpaste, a powdered toothpaste, or a liquid toothpaste. sulfate esters) and N-long chain acylamino acids or their salts (N-long chain acylamino acids) in a weight ratio of 1:2 to 2:1. In this way, higher alcohol sulfates and N
- By using together with long-chain acylamino acids at a weight ratio of 1:2 to 2:1, residual dextranase activity can be increased, and the usability and stability can be improved. You can, but
When higher alcohol sulfate esters are blended in an amount exceeding twice the amount of N-long chain acylamino acids (i.e.,
If the amount of N-long chain acylamino acids is less than half of the amount of higher alcohol sulfate esters), the stability of dextranase will be poor (see experimental examples described later), and the object of the present invention will not be achieved. Furthermore, if the amount of higher alcohol sulfate esters is less than half of the amount of N-long chain acylamino acids, it is not preferable because a large amount of N-long chain acylamino acids cannot be blended, resulting in poor foaming power. The amount of N-long chain acylamino acids blended is 0.2 to 0.5% (weight%, same hereinafter) of the entire toothpaste composition.
In particular, when N-lauroyl sarcosine sodium is used as the N-long chain acylamino acid, the upper limit of the amount to be blended should be 0.5% from the viewpoint of peeling off the oral mucosa. Furthermore,
From the viewpoint of usability and dextranase residual rate, the total amount of higher alcohol sulfate esters and N-long chain acylamino acids should be 0.5% or more of the total, especially 0.5 to 1.5%.
%. Examples of the higher alcohol sulfuric esters that can be used in the present invention include sulfuric esters of higher alcohols having 8 to 20 carbon atoms, more preferably 10 to 18 carbon atoms, and water-soluble salts thereof. Two or more types may be used in combination. Note that the higher alcohol may be a mixed higher alcohol in which two or more of these higher alcohols are mixed, and a sulfuric acid ester of this mixed higher alcohol may also be used. Specifically, sodium lauryl sulfate, sodium myristyl sulfate, sodium coconut oil sulfate, etc. can be used as higher alcohol sulfate esters, and sodium lauryl sulfate is particularly preferably used. N-long chain acylamino acids have a carbon number of 8
One or more N-substituted amino acids or water-soluble salts thereof with up to 20 saturated or unsaturated acyl groups are preferably used. The above acyl groups include capryl, lauroyl, tridecanoyl, 2
-Methyl lauroyl, myristoyl, pentadecanoyl, palmitoyl, stearoyl, isostearoyl, oleoyl and the like. Further, it may be a mixture such as a coconut oil fatty acid residue, a palm oil fatty acid residue, a hydrogenated beef fatty acid residue, or a mixed fatty acid residue of coconut oil and hydrogenated beef tallow. Amino acids include glutamic acid, glycine, alanine, β-
Examples include alanine, phenylalanine, leucine, isoleucine, methionine, proline, tryptophan, valine, serine, etc., and salts include sodium salt, potassium salt, etc. Furthermore, N-lower alkyl substituted products such as sarcosine and N-methyl-β-alanine can also be used. Particularly preferred N-long chain acylamino acids are alkali metal salts of glutamic acid, sarcosine, and N-methyl-β-alanine which are N-substituted with a saturated or unsaturated acyl group having 10 to 18 carbon atoms; Cosine sodium may be most preferably used. In addition, in the present invention, other surfactants may be added in addition to the above-mentioned surfactants, and in particular, fatty acid diethanolamides such as lauric acid diethanolamide, maltitol fatty acid esters, lactitol fatty acid esters, sucrose fatty acid esters, polyesters, etc. Nonionic surfactants such as oxyethylene fatty acid esters are combined with higher alcohol sulfate esters and N-
20-150 relative to the total weight of long-chain acylamino acids
%, thereby further improving the stability of dextranase. In the present invention, the amount of dextranase blended is not particularly limited, but usually per 1 g of oral composition.
100 to 100,000 units (u/g of oral composition), particularly preferably 1,000 to 50,000 u/g of oral composition. Here, 1 unit refers to the amount of enzyme that produces free reducing sugars equivalent to 1 μg of glucose per minute when the reaction is performed using dextran as a substrate. In this case, in the present invention, in addition to dextranase as an active ingredient, amylase, protease,
Enzymes such as mutanase, lysozyme, and Litetsuque enzyme; alkali metal monofluorophosphates such as sodium monofluorophosphate; fluorides such as sodium fluoride and stannous fluoride;
One or more active ingredients such as tin compounds, chlorhexidine salts, epsilon aminocaptonic acid, tranexamic acid, aluminum chlorohydroxylalantoin, dihydrocholesterol, glycyrrhizin salts, sodium chloride, and water-soluble inorganic phosphoric acid compounds may be blended. In particular, when an alkali metal monofluorophosphate such as sodium monofluorophosphate is used in combination with dextranase, it stabilizes dextranase and maintains a high residual rate of dextranase in the dentifrice composition after long-term storage. It is desirable because it can be done. In addition, when dextranase and mutanase are combined, a synergistic effect occurs to dissolve plaque and prevent re-deposition, and the combination of dextranase and Litetsuque enzyme has benefits such as strengthening the efficacy of Liteque enzyme. be. Note that the water-soluble inorganic phosphoric acid compound includes potassium salts or sodium salts of orthophosphoric acid, pyrophosphoric acid, and polyphosphoric acid, and potassium salts are preferred. The dentifrice composition of the present invention can further contain various other ingredients depending on the type thereof, and can be obtained as toothpaste, moist toothpaste, powdered dentifrice, liquid dentifrice, etc. according to the usual manufacturing method. Can be done. That is, other components include dicalcium phosphate dihydrate and anhydride, calcium carbonate, calcium pyrophosphate, insoluble sodium metaphosphate, anhydrous silicic acid, hydrated silicic acid, aluminosilicate, alumina, aluminum hydroxide, and tertiary phosphoric acid. One or more types of abrasives such as magnesium, magnesium carbonate, calcium sulfate, and synthetic resins may be blended (the blending amount is usually 10 to 90% of the total, especially 20 to 60% in the case of toothpaste). In addition, carrageenan, sodium carboxymethyl cellulose, sodium alginate, gums, polyvinyl alcohol, vegum,
One or more types of hydroxyethyl cellulose and the like may be blended (compounding amount usually 0.3 to 5%). Furthermore, sorbitol, glycerin,
One or more of propylene glycol, 1,3-butylene glycol, polyethylene glycol, xyrite, maltite, lactite, etc. may be blended (the blending amount is usually 10 to 70%). In this case, it is desirable to use propylene glycol as the binder dispersion, but if a large amount is blended, the dextranase residual rate during storage will decrease, so sorbit is blended as the main thickener and propylene glycol and sorbit are used in combination. It is desirable to do so. Furthermore, flavoring agents such as l-menthol, anethole, carvone, and eugenol, sweeteners such as saccharin sodium, stevioside, neohesperidine dihydrochalcone, thaumatin, glycyrrhizin, and perillartine, preservatives such as parabens, gelatin, peptone, and other ingredients. For example, when manufacturing a toothpaste, it can be manufactured by kneading the above-mentioned desired components with an appropriate amount of water. The toothpaste composition thus obtained is stored in a predetermined container such as an aluminum tube, a laminate tube made by laminating both sides of aluminum foil with plastic, a plastic tube, a bottle-shaped container, an aerosol container, etc. provided for use. The dextranase-containing dentifrice composition of the present invention includes:
By blending higher alcohol sulfate ester and N-long chain acylamino acids in a ratio of 1:2 to 2:1, dextranase is stored stably for a long period of time, and dextranase residual activity is high. The dentifrice composition itself has good stability, has good foaming properties, has a good feeling of use, and has good stability to the oral mucosa. Next, examples will be shown to specifically explain the effects of the present invention. Experimental Example Toothpaste compositions (toothpastes of the present invention, comparative toothpastes A and B) having the following formulations were prepared by a conventional method, and their usability (foaming), stability, and residual dextranase activity were compared.

[Table] From the above results, the toothpaste of the present invention has excellent residual dextranase activity, and has a good feeling of use.
It was also found to be good in terms of stability. Next, in the above treatment, the feeling of use and dextranase of toothpaste compositions in which the amount of sodium lauroyl sarcosine was 0.5% and the amount of sodium lauryl sulfate was changed to 0.2%, 0.3%, 0.5%, 0.9%, and 1.2%. Similar evaluations of residual activity showed that those with a sodium lauryl sulfate content of 0.2% had poor foaming power, while those with a sodium lauryl sulfate content of 1.2% were good in terms of feel when used, and those with a sodium lauryl sulfate content of 1.2% had poor foaming power. Stranase residual rate is low, and 0.9% or less is good from the standpoint of dextranase residual activity. It was found that the blending ratio with acyl amino acids) needs to be 1:2 to 2:1 (weight ratio). In addition, as shown in the examples described below, it is also good to use sodium coconut oil sulfate or sodium myristyl sulfate instead of sodium lauryl sulfate, and use sodium lauroyl glutamate or sodium lauroyl-β-alanine instead of sodium lauroyl sarcosine. It had significant residual dextranase activity. Examples are shown below. Example 1 Toothpaste dicalcium phosphate 50.0% Sorbit 20.0 Sodium lauryl sulfate 0.7 Sodium lauroyl sarcosine 0.5 Lauric acid diethanolamide 1.2 Carrageenan 1.0 Sodium saccharin 0.1 Flavor 1.0 Dextranase 2000u/g Toothpaste water Remaining 100.0% Example 2 Toothpaste Toothpaste Aluminum hydroxide 50.0% Silicic anhydride 2.0 Sorbit 15.0 Glycerin 15.0 Sodium coconut oil sulfate 0.5 Sodium lauroyl glutamate 0.5 Sodium alginate 1.0 Gelatin 0.5 Sodium saccharin 0.1 Fragrance 1.0 Dextranase 5000u/g Toothpaste water Remaining 100.0% Examples 3 Toothpaste Calcium Carbonate 50.0% Propylene Glycol 2.0 Sorbit 20.0 Sodium Myristyl Sulfate 0.5 Sodium Lauroyl-β-Alanine 0.3 Carrageenan 0.5 Sodium Alginate 0.5 Sodium Carboxymethyl Cellulose 0.3 Sodium Monofluorophosphate 0.76 Sodium Satucalin 0.1 Flavor 1.0 Dextranase 1000u/g Toothpaste water remaining 100.0% Example 4 Toothpaste dicalcium phosphate 50.0% Glycerin 20.0 Sodium lauryl sulfate 0.3 Sodium lauroyl sarcosine 0.5 Lactitol laurate monoester 0.4 Carrageenan 1.0 Sodium saccharin 0.1 Flavor (peppermint type) 1.0 Dextranase 20000u/g Toothpaste water remaining 100.0% Example 5 Hyunsei toothpaste calcium carbonate 80.0% Glycerin 10.0 Sodium lauryl sulfate 0.3 Sodium lauroyl sarcosinate 0.4 Satucharin sodium 0.1 Flavor 1.0 Dextranase 1500u/g Toothpaste water remaining 100.0% Example 6 Liquid Toothpaste glycerin 35.0% Sodium myristyl sulfate 1.0 Sodium lauroyl glutamate 0.5 Sodium saccharin 0.1 Ethanol 3.0 Fragrance 1.0 Sodium polyacrylate 5.0 Dextranase 3000u/g Toothpaste water Remaining 100.0% The oral compositions according to each of the above examples All of them had good residual dextranase activity and an excellent feeling of use.

Claims (1)

[Claims] 1. In a dentifrice composition containing dextranase, higher alcohol sulfate or its salt and N-long chain acylamino acid or its salt are mixed in a weight ratio of 1:2 to 2:1. A dentifrice composition characterized in that it is used in combination. 2. The dentifrice composition according to claim 1, wherein the amount of the N-long chain acylamino acid or its salt is 0.2 to 0.5% by weight of the total weight. 3. The dentifrice composition according to claim 1 or 2, wherein the higher alcohol sulfate ester or its salt is sodium lauryl sulfate, and the N-long chain acylamino acid or its salt is sodium lauroyl sarcosine. 4. Any one of claims 1 to 3 in which 20 to 150% by weight of a nonionic active agent is blended based on the total weight of higher alcohol sulfate ester or its salt and N-long chain acylamino acid or its salt. The dentifrice composition described.