JPS6257217B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an electrophoretic analysis method and apparatus, and in particular, to an electrophoretic analysis method and an apparatus for analyzing target components separated by electrophoresis.
The object of the present invention is to provide an electrophoretic analysis method and apparatus that allow electrophoresis to be performed again under different analysis conditions without taking the sample out of the system.

Conventionally, when precisely analyzing a target component in a sample consisting of multiple components, the sample is first subjected to electrophoresis under certain analysis conditions, and the separated target components are taken out of the system using a means such as a syringe. Then, after changing the analysis conditions to be suitable for the target component, the fractionated target component was injected again and electrophoresis was performed.

However, such operations are very troublesome, and there is a risk that part of the target component may be lost during the time between separating the target component out of the system and injecting it again.

This invention was made for the purpose of solving the above-mentioned problems, namely:
In a device that separates an injected sample by electrophoresis, the target component that has passed through a detector and is separated by electrophoresis is trapped in the middle of the electrophoresis tube, and the electrolyte is replaced and the applied voltage is changed. An electrophoretic analysis method in which a target component is migrated in the opposite direction toward a detector to precisely separate and detect the target component, and an apparatus for suitably implementing the method, that is,
A sample injection section, a detector, and a trap-drain switching means are sequentially connected by a conduit between electrode vessels connected to both ends of a high voltage power supply circuit, respectively, and between the sample injection section and the detector. The conduit and the conduit between the detector and the trap-drain switching means are constituted by an analytical migration tube, and the trap-drain switching means in one position connects the detector and the electrode on the detector side. To provide an electrophoresis analyzer which is configured such that a trap pipe is inserted between tanks, and a drain is inserted in place of the trap pipe at other positions.

This invention is effective for precisely separating a target component from a variety of coexisting charged substances, for example, in the analysis of charged substances in foods or biological samples.

Hereinafter, this invention will be explained in detail based on embodiments shown in the drawings. Note that this invention is not limited to this.

Reference numeral 1 shown in FIG. 1 is an embodiment of an electrophoretic analyzer according to the present invention. A first electrode tank 3 is connected to one end of the high voltage power supply circuit 2, and a second electrode tank 4 is connected to the other end.
A sample injection section 5, a detector 6, and a hexagonal cock 7 are connected in order between these electrode vessels 3 and 4 through conduits 8, 9, 10, and 11.

The detector 6 consists of a pair of potential gradient detectors juxtaposed with a short distance apart in the electrophoresis direction,
Detection of migration speed is also possible.

Pipe lines 9 and 10 are capillary reach tubes for analysis.

12 is a trap pipe, 13 and 14 are a drain stock, and 15 is a drain. At the first position of the hexagonal stock 7 (the flow path position in the stock shown by the solid line in FIG. 1), a trap pipe is installed between the pipes 10 and 11. 12 is inserted, and in the second position (the position of the flow path in the tank indicated by the dashed line in FIG. 1), the trap pipe 12 is closed and instead the drain 15 is
is inserted.

For analysis, first set the hexagonal pot 7 in the first position, fill the space between the first electrode tank 3 and the sample injection part 5 with terminal electrolyte T ₁ according to the usual procedure, and A leading electrolyte L ₁ is filled between the second electrode tank 4 and the sample injection section 5 . Thereafter, the sample S is injected into the sample injection section 5 and isokinetic electrophoresis is performed.

For example, the detector 6 obtains an output as shown in FIG. Since the time when component B enters the trap tube 12 can be estimated, the hexagonal cock 7 is switched to the second position at that time and the current is stopped at the same time.

Next, the drain stocks 13 and 14 are opened, and all of the electrolyte other than the part collected in the trap pipe 12 is discharged into the drain 15. and the first electrode tank 3
A new leading electrolyte L ₂ is filled from the second electrode tank 4 to the hexagonal pot 7, and a new terminal electrolyte T ₂ is filled between the second electrode tank 4 and the hexagonal pot 7, and the drain pots 13 and 14 are closed. .

After that, return the Roppo Kotoku 7 to the first position,
If electrophoresis is performed with the polarity of the high voltage power supply circuit 2 reversed, the target component B collected in the trap tube 12 will be separated more precisely, and the detector 6 will produce an output as shown in FIG. 3, for example. can get. Note that at this time, the signal direction at the detector 6 is reversed from that in FIG. 2.

As is clear from the above description, according to the electrophoretic analysis method and apparatus of the present invention, the target component is separated in the first electrophoresis, and only the target component is separated again without taking it out of the system. It is easy to perform a second electrophoresis, and furthermore, it is easy to change the analysis conditions such as changing the composition of the electrolyte, so precise analysis can be performed much more easily and reliably than before. can.

Note that the present invention can also be applied to electrophoresis that uses a single electrolytic solution without using a leading electrolytic solution and a terminal electrolytic solution.

Further, in the above embodiment, the time at which the target component enters the trap tube 12 was estimated from the migration speed measured by the detector 6, but the present invention is not limited to this. The entry of the target component into the trap tube 12 may be detected by. In this case, the detector 6 will be composed of one potential gradient detector or the like.

[Brief explanation of the drawing]

Fig. 1 is an explanatory diagram of the configuration of an embodiment of the electrophoresis analyzer of the present invention, Fig. 2 is an output diagram of the detector during the first electrophoresis, and Fig. 3 is the detector during the second electrophoresis. FIG. 1... Electrophoresis analyzer, 2... High voltage power supply circuit, 3... First electrode tank, 4... Second electrode tank,
5... Sample injection part, 6... Detector, 7... Roxagonal socket, 8, 9, 10, 11... Pipe line, 9, 10...
... Capillary reach tube, 12 ... Trap tube,
13, 14...Drain. 15...Drain.

Claims (1)

[Claims] 1. In a device that separates an injected sample by electrophoresis, a target component that has passed through a detector and been electrophoretically separated is trapped in the middle of the electrophoresis tube, and
An electrophoretic analysis method characterized by replacing the electrolytic solution and switching the applied voltage to cause the target component to migrate in the opposite direction toward the detector, thereby precisely separating and detecting the target component. 2 A sample injection section, a detector, and a trap-drain switching means are connected in order by a conduit between the electrode tanks connected to both ends of the high voltage power supply circuit, respectively, and between the sample injection section and the detector. and the pipe line between the detector and the trap-drain switching means are constituted by analytical migration tubes, and the trap-drain switching means, in one position, connects the detector and the trap-drain switching means on the detector side. Insert a trap tube between the electrode tanks,
An electrophoresis analyzer that is configured to have a drain inserted in place of the trap tube in other locations.