JPS6361906B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a fish culture composition for live bait. For aquaculture of yellowtail, yellowtail, sea bream, etc., raw fish such as saury, mackerel, anchovy, sardine, sand eel, and giant eel are often used as feed, such as whole, cut into chunks, or minced raw fish. Various vitamin supplements are added to fish and administered for the purpose of nutritional supplementation and disease prevention. However, some of these live feeds may exhibit strong thiaminase activity, and furthermore, the thiamine in the live feed and/or added to it is degraded by heat-stable reducing factors and other degradable factors. It is known to cause thiamine deficiency. Particularly in minced raw feed, thiamine is rapidly decomposed, so it is necessary to add a very large amount of thiamine [Green, RG et al., Journal of Nutrition, Vol. 21, p. 243 (1941 ); Sealock, RR et al., Journal of the American Chemical Society, vol. 65,
Page 935 (1943); Krampitz, LO et al., Journal of Biological Chemistry 152
Volume, page 9 (1944)]. In order to compensate for this drawback, it has been proposed that thiamine be coated with oil or the like to prevent it from decomposing in live bait [Special Publication No. 1973-
No. 13192] is not only expensive, but also cannot be said to be completely effective in preventing it. As a result of intensive research from a completely different perspective from the conventional technology, the present inventors found that O,S-dibenzoylthiamine is extremely stable in live feed for fish farming and is uniquely absorbed into the fish body. They obtained the unexpected finding that it was excellent in water and lasted for a long time, and proceeded with further research and completed the present invention. That is, the present invention is a fish culture composition for live bait containing O,S-dibenzoylthiamine or a salt thereof. In the present invention, O,S-dibenzoylthiamine may be used as a free base or as a physiologically acceptable salt, such as a mineral salt (hydrochloride, nitrate, sulfate, etc.). Such O,S-dibenzoylthiamine and its salts may be added to live feed as a single substance, but generally they are diluted in advance with excipients such as starchy substances such as wheat flour, gluten, brewer's yeast, fish meal, and compound feed. Preferably, it is used as a premix. It may also be used as a multivitamin mixed with other vitamins and minerals. Normally, it is preferable to ingest O,S-dibenzoylthiamine or its salt at about 1 to 20 mg per 1 kg of fish body weight (daily dose), and for this purpose, about 0.5 to 1000 mg, especially about 100 mg per 1 kg of live feed. It is best to add it at a rate of 2 to 200 mg. O,S-dibenzoylthiamine and its salts added to the live feed in this way are unique among various thiamine derivatives for a long time in the live feed for fish farming, as is clear from the experimental examples below. It is not time-resolved, is absorbed well into the fish body, and remains connected for a long time. In this way, the fish farming composition of the present invention
It has remarkable effects as a preventive treatment for _B1 deficiency and as a feed additive for fish farming. The present invention will be explained in more detail below using experimental examples and examples, but these are not intended to limit the present invention in any way. Experimental example 1 Mince saury and anchovy and add 5mg of O as thiamine per 100g of raw bait.
Add and mix S-dibenzoyl thiamine (hereinafter referred to as DBT), thiamine nitrate, benzoyl thiamine disulfide (hereinafter referred to as BTDS) hydrochloride or thiamine disulfide (hereinafter referred to as TDS) nitrate,
It was stored at 30°C, taken out at regular intervals, and the amount of remaining thiamine was determined by the thiochrome method. The result is the first
The results were as shown in Table 2. The numerical value is the percentage of the amount remaining relative to the amount of thiamine added.

【table】

[Table] Experimental Example 2 DBT hydrochloride or thiamine nitrate was forcibly administered orally as thiamine to yellowtail (1 kg body weight) at a dose of 10 mg/kg fish body weight, and the absorbability from the gastrointestinal tract was compared at a water temperature of 25°C. At regular intervals, fish were taken and 2 ml of blood collected from the heart was used to quantify the amount of thiamine in the blood in the same manner as in Experimental Example 1 without performing the reduction operation. The results are shown in Table 3.

[Table] Experimental example 3 DBT hydrochloride with lactose as thiamine 10mg/
After adding and mixing 10g of the powder adjusted to have a content of 10g to 4Kg of commercially available compound feed for yellowtail, it was mixed with 16Kg of anchovy mince to produce yellowtail weighing about 25g (3000 fish at the start, 2000 fish after 15 days, 30 1500 days later
1,000 fish after 60 days).
When the growth rate and survival rate up to 60 days were compared with the same amount of thiamine nitrate as thiamine instead of DBT hydrochloride, as shown in Table 4, the effect of DBT hydrochloride was far superior to that of thiamine hydrochloride. .

[Table] Experimental example 4 100g of frozen sardine thawed and minced
3 mg of O,S-dibenzoylthiamine (hereinafter referred to as DBT) hydrochloride as thiamine, O,S
- diacetylthiamine (hereinafter referred to as DAT) hydrochloride,
O,S-difluorylthiamine (hereinafter referred to as DFT)
Or add thiamine nitrate, mix and at 30℃
After being stored for 180 minutes, it was taken out and the amount of remaining thiamine was determined by the thiochrome method. The results were as shown in Table 5. The numerical value is the percentage of the amount remaining relative to the amount of thiamine added. From Table 5, DBT shows the highest residual amount, especially among O,S-diacylthiamines.
It is clear that DBT is uniquely and significantly stable in live feed.

[Table] Example 1 Powder prepared using feed brewer's yeast so that DBT hydrochloride was 10 mg/g. This product is 1 fish weight per day.
It is best to administer it by mixing or spreading it into live feed at a rate of 0.3g per kg. Example 2 Vitamin A oil in addition to 1.5g of DBT hydrochloride per 1Kg
1 million units, vitamin D ₃ oil 200,000 units, acetic acid DL-α
-Tocopherol 3.0g, Menadione bisulfite dimethylpyrimidinol 0.1g, Riboflavin 1.8
g, nicotinamide 3.0g, pyridoxine hydrochloride
2.2g, D-calcium pantothenate 20.0g, d
-Biotin 2.0mg, para-aminobenzoic acid 0.3g, inositol 1.0g, choline chloride 10.0g, folic acid 0.3g,
Composition consisting of 2.0 mg of cyanocobalamin, 14.5 g of calcium L-ascorbate, 3.0 g of DL-methionine, and residual excipients. Administer 200g of live bait mixed with this product at a rate of 0.5% per 1kg of fish weight per day.

Claims (1)

[Claims] 1 A fish culture composition for live bait containing O,S-dibenzoylthiamine or a salt thereof.