JPH052307B2 - - Google Patents
Info
- Publication number
- JPH052307B2 JPH052307B2 JP24450084A JP24450084A JPH052307B2 JP H052307 B2 JPH052307 B2 JP H052307B2 JP 24450084 A JP24450084 A JP 24450084A JP 24450084 A JP24450084 A JP 24450084A JP H052307 B2 JPH052307 B2 JP H052307B2
- Authority
- JP
- Japan
- Prior art keywords
- inoculation
- line
- valve
- pot
- steam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000011081 inoculation Methods 0.000 claims description 67
- 101100298225 Caenorhabditis elegans pot-2 gene Proteins 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】
〔発明の利用分野〕
本発明は、発酵槽の植菌装置に関するもので、
特にバイオハザード対策設備の発酵槽に適する植
菌装置に関するものである。
〔発明の背景〕
従来の発酵槽の植菌方法は、火炎植菌方法又は
注射植菌方法のいずれかであり、これらはいずれ
も植菌する場合、その構造上種菌中の微生物が周
囲の外気、あるいは植菌作業を行なう作業者を汚
染するという欠点があり、バイオハザード対策を
行なつた設備の中で使用することが難かしく、特
に微生物が周囲の生物系に対し危険な場合には、
従来方法の植菌装置は使用できなかつた。
従来の火炎植菌については、例えば「発酵工
業」木下祝郎著大日本図書株式会社昭和50年７月
１日発行、95頁において論じられている。
〔発明の目的〕
本発明の目的は、種菌を無菌的に、かつ、植菌
中の微生物が周囲を汚染するのを防止した発酵槽
の植菌装置を提供することにある。
〔発明の概要〕
本発明は、発酵槽の植菌口に植菌バルブを介し
て接続した植菌ラインにドレンラインを接続し、
前記植菌ラインと加熱殺菌用蒸気および冷却、圧
押し用無菌空気を供給する蒸気・無菌空気供給ラ
インとの間に、バイパスラインを備えた植菌ポツ
トユニツトを着脱可能に接続して、種菌を無菌的
に、かつ、植菌中の微生物が周囲の環境を汚染す
ることなく、発酵槽内に植菌するようにしたもの
である。
〔発明の実施例〕
以下、本発明の実施例を図面により説明する。
発酵槽１の上部の植菌口１３には植菌バルブ１２
を介して植菌ライン１６が接続されており、植菌
ライン１６にはドレンバルブ１０を介してドレン
ライン１１が接続されている。２は上蓋１５をネ
ジ結合等により着脱可能に装着した植菌ポツト
２、上蓋１５に圧押しバルブ３、Ｔ継手１７を介
して取付けられたカツプラー９、植菌ポツト２の
下部にバルブ４を介して取付けられたカツプラー
８、バルブ４よりバイパスライン６を介してＴ継
手１７に接続されたカツプラー７よりなる植菌ポ
ツトユニツト、１４は加熱殺菌用蒸気および冷
却、圧押し用無菌空気を供給する蒸気・無菌空気
供給ラインであつて、植菌ライン１６と蒸気・無
菌空気供給ライン１４の間にカツプラー８，９を
介して植菌ポツトユニツト２が着脱可能に接続さ
れている。
上述の構成において、種菌の発酵槽１内への植
菌について説明する。まず、あらかじめオートク
レーブ（図示せず）で殺菌された植菌ポツト２、
上蓋１５、圧押しバルブ３、Ｔ継手１７、バルブ
４，５、バイパスライン６、カツプラー７，８，
９よりなる植菌ポツトユニツト２を安全キヤビネ
ツト（図示せず）の中に入れ、カツプラー７を離
脱して上蓋１５を取外す。つぎに、圧押しバルブ
３およびバルブ４が閉じていることを確認した
後、植菌ポツト２内に種菌を入れ蓋１５を取付
け、カツプラー７を結合して植菌ポツトユニツト
２を安全キヤビネツトより取出し、カツプラー８
を植菌ライン１６に接続し、カツプラー９を蒸
気・無菌空気供給ライン１４に接続する。しかし
て、バルブ５およびドレンバルブ１０を開いて、
蒸気・無菌空気供給ライン１４よりカツプラー
９，７、バイパスライン６、バルブ５を経て植菌
ライン１６に蒸気を供給し、ドレンバルブ１０を
介してドレンライン１１より排出することによ
り、植菌ライン１６を殺菌した後、無菌空気を供
給して植菌ライン１６を冷却する。植菌ライン１
６の冷却が完了すると、バルブ５、ドレンバルブ
１０を閉じ、圧押しバルブ３を開いて植菌ポツト
２内に無菌空気を供給して加圧し、バルブ４、植
菌バルブ１２を開いて植菌ポツト２内の種菌を発
酵槽１内に投入植菌する。発酵槽１への植菌が終
了すると、上述と同様に植菌ライン１６の殺菌を
行なつた後、カツプラー８，９を離脱させて植菌
ポツトユニツト２を発酵槽１より取外す。
〔発明の効果〕
本発明は以上述べたように、発酵槽の植菌口に
植菌バルブを介して植菌ラインを接続し、前記植
菌ラインにドレンバルブを介してドレンラインを
接続し、前記植菌ラインと蒸気・無菌無気供給ラ
インとの間にバイパスラインを備えた植菌ポツト
ユニツトを着脱可能に接続したものであるから、
植菌操作中種菌を完全に無菌的に保持するとがで
きると共に、植菌中の微生物が周囲の環境を汚染
するのを未然に防止することができ、バイオハザ
ードの心配が全くない発酵槽の植菌装置を得るこ
とができる。 [Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to an inoculation device for a fermenter,
In particular, it relates to an inoculation device suitable for fermenters in biohazard countermeasure equipment. [Background of the Invention] The conventional inoculation method for fermenters is either the flame inoculation method or the injection inoculation method. Alternatively, it has the disadvantage of contaminating the workers performing the inoculation work, making it difficult to use in facilities that have taken biohazard measures, especially when the microorganisms are dangerous to the surrounding biological system.
Conventional inoculation devices could not be used. Conventional flame inoculation is discussed, for example, in "Hakko Kogyo" by Yoshiro Kinoshita, published by Dainippon Tosho Co., Ltd., July 1, 1975, p. 95. [Object of the Invention] An object of the present invention is to provide a fermenter inoculation device that aseptically inoculates seed bacteria and prevents microorganisms being inoculated from contaminating the surrounding area. [Summary of the invention] The present invention connects a drain line to an inoculation line connected to an inoculation port of a fermenter via an inoculation valve,
An inoculation pot unit equipped with a bypass line is removably connected between the inoculation line and a steam/sterile air supply line that supplies steam for heat sterilization and sterile air for cooling and pressing to sterilize the inoculum. The microorganisms being inoculated can be inoculated into the fermenter without contaminating the surrounding environment. [Embodiments of the Invention] Examples of the present invention will be described below with reference to the drawings.
An inoculation valve 12 is installed in the inoculation port 13 at the top of the fermenter 1.
An inoculation line 16 is connected to the inoculation line 16 via a drain valve 10, and a drain line 11 is connected to the inoculation line 16 via a drain valve 10. 2 is an inoculation pot 2 with an upper lid 15 removably attached by screw connection, a press valve 3 on the upper lid 15, a coupler 9 attached via a T-joint 17, and a valve 4 attached to the bottom of the inoculation pot 2. The inoculation pot unit consists of a coupler 8 attached to the tube, a coupler 7 connected from the valve 4 to a T-joint 17 via a bypass line 6, and a steam/inoculation pot unit 14 that supplies steam for heat sterilization and sterile air for cooling and pressing. The inoculation pot unit 2 is detachably connected between the inoculation line 16 and the steam/sterile air supply line 14 via couplers 8 and 9, which are sterile air supply lines. In the above-described configuration, inoculation of seed bacteria into the fermenter 1 will be explained. First, inoculation pot 2, which has been sterilized in advance in an autoclave (not shown),
Upper lid 15, pressure valve 3, T joint 17, valves 4, 5, bypass line 6, couplers 7, 8,
The inoculation pot unit 2 consisting of 9 is placed in a safety cabinet (not shown), the coupler 7 is removed, and the top cover 15 is removed. Next, after confirming that the pressure valve 3 and valve 4 are closed, put the seed bacteria into the inoculation pot 2, attach the lid 15, connect the coupler 7 , and take out the inoculation pot unit 2 from the safety cabinet. Katsu puller 8
is connected to the inoculation line 16, and the coupler 9 is connected to the steam/sterile air supply line 14. Then, open the valve 5 and the drain valve 10,
Steam is supplied from the steam/sterile air supply line 14 to the inoculation line 16 via the couplers 9, 7, the bypass line 6, and the valve 5, and is discharged from the drain line 11 via the drain valve 10. After sterilizing the inoculation line 16, sterile air is supplied to cool the inoculation line 16. Inoculation line 1
When cooling of step 6 is completed, valve 5 and drain valve 10 are closed, press valve 3 is opened to supply and pressurize sterile air into inoculation pot 2, and valve 4 and inoculation valve 12 are opened to inoculate. The inoculum in the pot 2 is inoculated into the fermenter 1. When the inoculation into the fermenter 1 is completed, the inoculation line 16 is sterilized in the same manner as described above, and the couplers 8 and 9 are removed to remove the inoculation pot unit 2 from the fermenter 1. [Effects of the Invention] As described above, the present invention connects an inoculation line to the inoculation port of a fermenter via an inoculation valve, connects a drain line to the inoculation line via a drain valve, An inoculation pot unit equipped with a bypass line is removably connected between the inoculation line and the steam/sterile air supply line,
The inoculum can be kept completely sterile during the inoculation process, and the microorganisms being inoculated can be prevented from contaminating the surrounding environment. A bacterial device can be obtained.
図面は本発明による発酵槽の植菌装置の一実施
例を示す系統図である。
１……発酵層、２……植菌ポツト、２……植菌
ポツトユニツト、３……圧押しバルブ、４，５…
…バルブ、６……バイパスライン、７〜９……カ
ツプラー、１０……ドレンバルブ、１１……ドレ
ンライン、１２……植菌バルブ、１３……植菌
口、１４……蒸気・無菌空気供給ライン、１５…
…蓋、１６……植菌ライン、１７……Ｔ継手。
The drawing is a system diagram showing an embodiment of the inoculation device for a fermenter according to the present invention. DESCRIPTION OF SYMBOLS 1... Fermentation layer, 2... Inoculation pot, 2 ... Inoculation pot unit, 3... Pressure valve, 4, 5...
...Valve, 6...Bypass line, 7-9...Cup puller, 10...Drain valve, 11...Drain line, 12...Inoculation valve, 13...Inoculation port, 14...Steam/sterile air supply Line, 15...
...Lid, 16...Inoculation line, 17...T-joint.
Claims (1)
菌ライン１６を接続すると共に、該植菌ライン１
６の植菌バルブ１２の前段で分岐させてドレンバ
ルブ１０を介してドレンライン１１を接続し、該
植菌ライン１６の上流に、上蓋を着脱可能に装着
した植菌ポツト２と、該植菌ポツト２の上部・下
部にそれぞれバルブ３，４を介してカツプラー
９，８を接続すると共に、上部のバルブ３とカツ
プラー９間及び下部のバルブ４とカツプラー８間
をそれぞれ分岐させ、該分岐間をバルブ５を介し
て接続するバイパスライン６とからなる植菌ポツ
トユニツト２を着脱可能に接続し、該植菌ポツト
ユニツト２の上流に蒸気・無菌空気供給ライン１
４を接続し、バルブ５及びドレンバルブ１０を開
いて、前記蒸気・無菌空気供給ライン１４と、バ
イパスライン６とを介して植菌ライン１６に蒸
気・無菌空気を供給し、前記ドレンライン１１よ
り蒸気・無菌空気を排出し、植菌ライン１６を殺
菌、冷却するように構成したことを特徴とする発
酵槽の植菌装置。1 Connect the inoculation line 16 to the inoculation port of the fermenter via the inoculation valve 12, and connect the inoculation line 1 to the inoculation port of the fermenter.
The inoculation pot 2 is connected to the drain line 11 via the drain valve 10, and the inoculation pot 2 is connected to the inoculation line 11 at the front stage of the inoculation valve 12 of No. Couplers 9 and 8 are connected to the upper and lower parts of the pot 2 via valves 3 and 4, respectively, and the valve 3 and the coupler 9 in the upper part and the valve 4 and the coupler 8 in the lower part are branched, respectively, and a connection is made between the branches. An inoculation pot unit 2 consisting of a bypass line 6 connected via a valve 5 is detachably connected, and a steam/sterile air supply line 1 is connected upstream of the inoculation pot unit 2 .
4 and open the valve 5 and drain valve 10 to supply steam and sterile air to the inoculation line 16 via the steam and sterile air supply line 14 and the bypass line 6, and from the drain line 11. A fermentation tank inoculation device characterized by being configured to discharge steam and sterile air to sterilize and cool an inoculation line 16.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24450084A JPS61124372A (en) | 1984-11-21 | 1984-11-21 | Fermenter inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24450084A JPS61124372A (en) | 1984-11-21 | 1984-11-21 | Fermenter inoculation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61124372A JPS61124372A (en) | 1986-06-12 |
| JPH052307B2 true JPH052307B2 (en) | 1993-01-12 |
Family
ID=17119592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24450084A Granted JPS61124372A (en) | 1984-11-21 | 1984-11-21 | Fermenter inoculation device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61124372A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0659207B2 (en) * | 1986-03-10 | 1994-08-10 | キッコーマン株式会社 | Solid medium sterilizer |
| JPH0616559Y2 (en) * | 1987-03-13 | 1994-05-02 | 株式会社日立製作所 | Additive device for fermenter |
| JPH074223B2 (en) * | 1988-03-30 | 1995-01-25 | 株式会社日立製作所 | Method and device for supplying chemical liquid for culture tank |
| JPH0646937B2 (en) * | 1988-10-17 | 1994-06-22 | 雪印乳業株式会社 | Concentrator for culture fluid |
| JPH0585298U (en) * | 1992-04-27 | 1993-11-19 | 株式会社千代田製作所 | Sampling valve device for culture device |
-
1984
- 1984-11-21 JP JP24450084A patent/JPS61124372A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61124372A (en) | 1986-06-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |