JPH074223B2 - Method and device for supplying chemical liquid for culture tank - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a method and an apparatus for supplying a chemical solution in a culture tank, and particularly consumes a large amount of a chemical solution such as an acid, an alkali and an antifoaming agent during a culture operation. The present invention relates to a method and an apparatus for supplying a chemical liquid for a culture tank, which is suitable for a culture system.

[Conventional technology]

Conventionally, when a chemical solution such as an acid, an alkali or an antifoaming agent in a culture tank is insufficient during the culture, the chemical solution is individually sterilized in an autoclave and supplied by a flame injection method. Further, although the supplies are different, when aseptically supplying (inoculating) the inoculum to the culture tank, the flame inoculation method was used. A device related to this type of device is, for example, JP-A-60-110281.

[Problems to be Solved by the Invention]

The above-mentioned prior art does not consider a large amount of the chemical solution and the culture system, and the handling at the time of supplying the chemical solution to the chemical solution pot is by introducing the flame as described above, and its operation is very troublesome, and the outside air There was a problem that various bacteria in it were mixed.

An object of the present invention is to provide a method and an apparatus for supplying a chemical liquid for a culture tank, which can improve the operability when supplying the chemical liquid to the chemical liquid pot and can improve the sterility.

[Means for Solving the Problems]

The above object is to provide a culture tank and a jacketed chemical solution pot for supplying a chemical solution such as an acid, an alkali or an antifoaming agent to the culture tank in parallel with a chemical solution supply line and an air supply line passing through a pressure equalizing line. Configured via an automatic valve, the temperature of the jacketed chemical solution pot is controlled by the steam line and the cooling line using the automatic valve, and the chemical solution is supplied to the jacketed chemical solution pot through the automatic valve provided in each line. It is achieved by controlling with a sequencer.

[Action]

Since each line of the jacketed chemical solution pot and the culture tank is a flow path that communicates via an automatic valve, each automatic valve is controlled by a sequencer, and when the chemical solution is supplied during the culture operation, the culture tank body and the jacket are controlled. Cut off the flow path to the chemical liquid pot. Therefore, the sterility of each line can be secured, and the sterility is further improved by operating the steam line for automatic sterilization. Further, the temperature control of the jacketed chemical solution pot can be controlled by a sequencer by a steam line, a cooling line, and a temperature signal converter equipped with a temperature sensor. Further, since all the above operations can be controlled by the sequencer, operability can be improved.

〔Example〕

 An embodiment of the present invention will be described below with reference to FIG.

In the figure, 1 is a chemical solution pot with a jacket, 2 is a culture tank, 3 is an air filter, and 4 is a sequencer.
The jacketed chemical liquid pot 1 flows from the upper part to the culture tank 2 through a pressure equalizing line 7 ′, a pressure equalizing line sluice valve 8 (automatic valve), a pressure equalizing line 7, an automatic valve 21, and an air supply line 23. There is a road. In addition, the chemical liquid supply line 24 that comes out from the bottom of the chemical liquid pot 1 with a jacket is provided with automatic valves 14 and 16
A flow path is provided to flow to the culture tank 2 via the. further,
An exhaust line 25 using the automatic valve 10 is provided above the jacketed chemical liquid pot 1. Further, a steam line 26 for adjusting the temperature of the jacketed chemical liquid pot 1, a cooling line 27, 27 ', and a sterilization line 28, 29 are provided by using automatic valves 13, 11, 12, 15, 15, respectively. There is. The drain lines 30 and 31 are provided by using automatic valves 17 and 18, respectively.

In order to control the temperature of the jacketed chemical solution pot 1, a temperature sensor 6 is provided in the jacketed chemical solution pot 1, and is connected to the sequencer 4 via a temperature signal converter 5 and a signal line 32. Also, in order to supply sterile air to the culture tank 2,
The pressure equalizing line 7 is communicated with the air filter 3 (bactericidal filter).

In the above configuration, the automatic valves 8 and 21 are opened when the culture tank 2 is in operation, and the pressure equalizing rice 7 and 7'and the air supply line 23 are used to vent (flow) and supply (supply) the chemical solution to the culture tank 2.
The pressurizing air is supplied to. Automatic valve 16 is culture tank 2
The valve opening is automatically controlled by fluctuations in the pH and bubble surface inside.

Select the sterilization preparation mode (push button type) when there is no drug solution in the jacketed drug solution pot 1 during culturing and a new drug solution is added to the jacketed drug solution pot 1 for sterilization.
Then, the sequencer 4 forcibly closes the automatic valve 8 and the automatic valve 16, and the flow path with the culture tank 2 system drops.

At this time, the automatic valve 10 of the exhaust line 25 of the jacketed chemical solution pot 1 is opened, the internal pressure of the jacketed chemical solution pot 1 is released, and the chemical solution can be supplied (input).

Select the sterilization mode (push button type) after chemical solution supply (input)
Then, steam lines 28, 28 ', 29 automatic valves 9, 14, 1
5, 20 and the drain lines 30, 31 automatic valves 17, 18 and the exhaust line 25 automatic valve 10 operate according to the sequencer 4 to start sterilization, and the temperature signal converter 5 and the temperature sensor 6 change the sterilization temperature. Take control.

After the sterilization, the automatic valves 11 and 12 of the cooling lines 27 and 27 'are operated to perform cooling, and the sterilization ends when the temperature is lowered to a predetermined temperature.

Next, by pressing the stop button, the automatic valve 8 of the pressure equalizing line 7'is opened, the automatic valve (chemical solution injection valve) 16 starts to operate, and the normal culture operation state is restored (the above selection mode button is the sequencer's Built into the control panel.).

Instead of the jacketed chemical solution pot of this embodiment, a chemical solution pot having a temperature control means using an in-can coil may be used. Further, a microcomputer may be used instead of the sequencer of this embodiment.

According to this embodiment, there is an effect that the operability of supplying (inputting) the chemical liquid and the sterility can be improved.

〔The invention's effect〕

According to the present invention, during the culture operation, the chemical solution can be supplied (additional injection) to the jacket-equipped chemical solution pot, and the automatic sterilization operation can be performed.

Therefore, it is not necessary to individually sterilize the chemical liquid in advance by autoclave and supply it to the pot by introducing the flame, so that the operability at the time of supplying the chemical liquid can be improved, and sterility can be ensured and improved.

[Brief description of drawings]

FIG. 1 is a schematic configuration diagram of a chemical liquid supply device for a culture tank according to an embodiment of the present invention. 1 ... Chemical pot with jacket, 4 ... Sequencer,
5 ... Temperature signal converter, 6 ... Temperature sensor, 8-18 ...
… Automatic valve

Front page continuation (72) Inventor Masakatsu Fujimoto 794, Higashi-Toyoi, Kudamatsu City, Yamaguchi Prefecture Inside the Kasado Factory, Hitachi Ltd. (56) References JP-A-58-47485 (JP, A) JP-A-61- 195682 (JP, A) JP-A-61-124372 (JP, A)

Claims (2)

[Claims] 1. When supplying a chemical solution such as an acid, an alkali or an antifoaming agent to a culture tank from a chemical solution supply pot, a chemical solution supply line for supplying the chemical solution from the chemical solution supply pot to the culture tank is provided. Close the automatic valve provided, and to the jacket provided on the outer periphery of the chemical solution supply pot and connected to the steam line and the cooling line, after supplying the chemical solution, supply steam from the steam line, and after this steam supply A method for supplying a chemical liquid for a culture tank, comprising supplying cooling water from the cooling line and opening the automatic valve. 2. A chemical liquid supply device for a culture tank comprising a culture tank and a chemical liquid supply pot for supplying a chemical liquid such as an acid, an alkali and an antifoaming agent to the culture tank via a chemical liquid supply line, wherein the chemical liquid supply pot is provided. From the jacket provided, the steam line and cooling line connected to this jacket, the automatic valve provided in the chemical solution supply chemical solution supply line, and the sequencer that sequence-controls the steam line, cooling line and automatic valve. When supplying the chemical solution to the pot, the sequencer closes the automatic valve before supplying the chemical solution, supplies steam from the steam line to the jacket, and cools water from the cooling line to the jacket. And a sequence control for opening the above-mentioned automatic valve to perform a chemical solution supply device for a culture tank.