JPH0610136B2 - Mutagenic substance poison performance remover contained in food and drink - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an agent for removing mutagen poisoning performance (digestion and absorption poisoning performance) contained in beverages by using dietary fiber contained in sugar beet pulp.

More specifically, the present invention relates to an agent for removing, in the digestive tract, the mutagenic substance poisoning ability (excluding digestion and absorption poisoning ability) contained in beverages by using dietary fiber contained in sugar beet pulp.

As the mutagen in the present invention, a chemical substance that is positive by an assay method such as the Ames method or a precursor that is converted into a chemical substance that is positive by the Ames method or the like in an animal body, for example, is converted to methylazoxymethanol 1 , 2-Dimethylhydrazine (Dietary Fiber, edited by the Japan Dietetic Society, May 15, 1982, published by Daiichi Publishing Co. p.p217-221 and Proc.Nat.Acad, Sci.USA V
ol.72, NO.12, December 1975 pp 5135-5139).

It has been previously announced that there is a toxicity-preventing effect on the toxic performance of mutagens in vegetables and the like. For example, “Environmental Mutagen Research” Vol. 3, 1981, p.
We have found that broccoli, burdock, etc. show inactivating / suppressing effects on mutagens such as heated combustion products of amino acids and proteins, and among them, cabbage factor is a kind of peroxidase. "Chemistry and Biology" Vol.18 NO.2, 1980, 9th
From page 5 to page 105, there are various descriptions of nutritional effects of dietary fiber. Dietary fiber prepared from burdock, carrot, radish, bean sprouts, etc. has a toxicity-inhibiting effect on harmful substances such as red No. 2 (Amaranth). There is.

Furthermore, the toxicity inhibition ability is very different depending on the type of dietary fiber, and the amaranth toxicity inhibition ability when burdock fiber is fractionated and prepared is holocellulose> neutral detergent treated DF (NDF)> burdock DF> crude sensation> acidic Detergent-treated DF (ADF)>
It becomes a class of cellulose, and pure cellulose prepared from cotton is completely ineffective. Regarding the relationship between the physicochemical properties of this dietary fiber and the effect, it shows a high correlation with water hydration (WHC) and water settling volume (SV), especially water settling volume (SV).
It is said that the larger the value, the greater the toxicity-inhibiting ability.

On the other hand, sugar beet pulp is either a sugar residue extracted from sugar beet during sugar beet production, which is compressed and dried, or is discharged outside the plant without being dried. However, the present invention has been proposed in Japanese Patent Application Laid-Open No. 56-32971, Japanese Patent Application Laid-Open No. 56-96658, etc. As described above, it is possible to make an edible high-fiber food by crushing and washing with water and then sieving, or by crushing and sieving after heat treatment.

This sugar beet pulp contains water-soluble and insoluble fibers such as pectin, hemicellulose, and cellulose, and is extremely excellent as a so-called dietary leaf eye bar.

The present inventors have variously studied the dietary fiber contained in the above sugar beet pulp, the enzyme is completely inactivated, and,
The present invention has been made based on the finding that the ability to remove mutagens poisoning performance is extremely large, even though the SV (water settling volume) is not large compared to other dietary fibers.

Sugar beet pulp is the residue after extraction of soluble components such as sugar from sugar beet as described above, and examples of the ingredients are as follows.

In the present invention, the compressed pulp as shown in the above table is dried and, if necessary, crushed and used as a powder.

Now, as a result of conducting tests on water retention and submerged volume of burdock fiber, butterbur fiber, wood cellulose, konjac mannan and sugar beet pulp dietary fiber which have been powdered with a particle size of 60 mesh, as shown in the table below. Atsuta

Test method Water retention: Centrifugal method is used. (Ii) Excess pure water is added to the sample, mixed well, and then naturally filtered to remove free water.

(B) A sample cylinder (100 ml volume) is packed with 100 ml of the sample from which free water is removed, and centrifugal dehydration is performed using a centrifugal filtration device (horizontal type).

(C) After centrifugal dehydration, determine the water content ratio (weight percent) of the sample.

Underwater settling volume: (a) Add about 50 ml of water to 1 g of dried sample, and then degas with a running water pump.

(B) Transfer this sample to a graduated cylinder, add a sufficient amount of water, and then measure the water settling volume as the swelling amount when water equilibrium is reached. Then, it becomes fluff fiber and burdock fiber, and sugar beet pulp dietary fiber does not have a particularly large value. That is, the sugar beet pulp dietary fiber used in the present invention has a sedimentation volume in water smaller than that of konjac mannan or burdock, butterbur, etc.

In addition, 1 of the fiber component composition of this sugar beet pulp dietary fiber
An example is as follows.

As shown in Table 3 above, the sugar beet pulp dietary fiber is composed of pectin, hemicellulose, cellulose and the like, and a water-soluble and insoluble composite fiber.

The present invention relates to a mutagen poison performance-eliminating agent containing as an active ingredient dietary fiber obtained from sugar beet pulp containing a composite fiber of water-soluble dietary fiber and insoluble dietary fiber.

In the present invention, the sugar beet pulp dietary fiber, which is a water-soluble and insoluble composite fiber, is orally ingested to adsorb the mutagen existing in the digestive tract and eliminate the mutagen poisoning performance. , Is discharged as it is outside the body.

Next, regarding the ability of these dietary fibers to remove the poisoning performance, Example 1
Using the sugar beet pulp dietary fiber powder (Betas: Trademark) obtained in step 1 and the fractions of each composition obtained therefrom, a mutagen toxic performance remover potency test was performed by the Ames method using each mutagen and salmonella. I went. The results are shown in Table 4 below.

Note 1. Betas is a product name (trademark) of sugar beet pulp dietary fiber powder manufactured by Nippon Sugar Beet Sugar Co., Ltd. 2. This test was conducted using the mutagen Trp-P-1 (3-amino-1,4-dimethyl-5H-pyrido [ 4,3-b] indole), Trp-P-2 (3-amino-1-methyl-5H-pyrido [4,3-b] indole),
Glu-P-1 (2-amino-6-methylpyrido [1,2-a: 3 ', 2'
-d] imidazole), Glu-P-2) 2-aminopyrido [1,2-
a: 3 ′, 2′-d] imidazole), and IQ (2-amino-3-
For methylimidazo [4,5-f] quinoline 0.3, 1.
Approximately 2% of sugar beet pulp dietary fiber Petas and its fraction were mixed with 0, 0.1, 1.0, 0.05 ppm aqueous solution as a dry matter and allowed to stand at room temperature, and after 1 hour, mutagenicity was determined by the Ames method (TA98 + S9).
Mix) to test the suppressive effect of mutations that cause carcinogenesis. The unit is the average number of colonies. However, the number of colonies spontaneously generated on the plate was removed.

3. The value in parentheses is the index with the mutagen as 100. As shown in Table 4, sugar beet pulp dietary fiber shows strong adsorption to carcinogenic mutagens, and It has a great restraint. That is, Trp
-In P-1, the rate of mutation is zero, and Glu-
25.4% for P-1, 17% for Glu-P-2, 5.9% for IQ
And it has a blocking effect that decreases sharply. Such a suppressive effect is considered to be because sugar beet pulp dietary fiber has an action of adsorbing carcinogens, and the beet pulp pulp fiber having a porous and high water retention property absorbs carcinogens and cannot activate it. .
Further, since this adsorptive power is strong, it is considered that the carcinogen is adsorbed in the body and is excreted and removed outside the body in the inactivated state. On the other hand, hemicellulose, which is a fractionated product,
Pectin, acidic detergent treated fiber (cellulose + lignin), neutral detergent treated fiber (hemicellulose + cellulose + lignin), etc. are all inferior in removal effect to sugar beet pulp dietary fiber. Nonetheless, in the present invention, the mutagenic substance toxicity-inhibiting effect is produced by the integrated action of the water-soluble and insoluble complex dietary fibers contained therein.

The sugar beet pulp dietary fiber which is an active ingredient of the present invention has a water content of 5%.
As a daily intake of 3 to 15 g / person / day is sufficient,
It is preferable to ingest the sugar beet pulp dietary fiber alone or in the state of being mixed or processed with various foods or drugs.

Next, examples will be described.

Example 1. In the sugar beet sugar-making step, 1 t of kozetsuto (water content 89.2%) that had been leached from the leaching tower was immediately treated with hot water at 95 ° C. and the product temperature was maintained at 90 ° C. or higher for 2 minutes. A dehydrator was used to obtain 373 kg of dehydrated sugar beet cabbage (water content 72.4%).

Then, it is dried with a drum rotary dryer to have a water content of 5.4%.
108 kg of dried sugar beet cabbage (ash content 3.2%) was obtained.

Then, this dried caustic is sieved with an 18 mesh sieve to remove 6 kg of all passables on the 18 mesh sieve, and the dried sugar beet kozette on the 18 mesh sieve is compacted (hammer mill type, screen mesh 150 mesh). Pulverized and screened 150 mesh sieve all-through powder (water content 5.3%, ash content 2.8)
%) 67 kg was obtained.

The thus-obtained differential sugar beet pulp was used as a mutagen poison performance removing agent (hereinafter referred to as BF) of the present invention.

Example 2 Male Wistar rats having a body weight of 50 g were fed with a basic feed, and about 1
After the weight was set to 00 g, the body weight was standardized, and the test was carried out by dividing 10 animals into 3 groups.

Casein 25%, Corn oil 5%, salt mixture 4%, Vitami
n mixture (vitamin mixture) 1%, Cholin Chloride
(Choline hydrochloride) 0.2%, Vitamin E granule (vitamin E granules) 0.05%, Sucrose (sucrose) 64.75% were added to the basic feed, two sets of basic feed were added, and 1,2-dimethylhydrazine was added. It was administered by a gastric tube once a week at a rate of 30 mg / kg body weight.

The BF-added feed prepared by adding 10% of BF obtained in Example 1 to the 3 sets of sucrose of the above-mentioned basic feed was fed to the 3 sets, and 1,2-dimethylhydrazine was administered in the same manner as the 2 sets. did.

Each feed was given ad libitum.

The two sets of rats had a poor rate of weight gain, and 4 rats died suddenly from liver disease after 9 weeks, but the 1 and 3 sets of rats were still healthy after 10 weeks and no death occurred.

Test Example 1 SD rats (male) having a body weight of about 50 g were bred with the basic feed of Example 2 to have a body weight of about 100 g, and then the body weight was standardized to obtain one set 10
Six test zones were prepared. Rats of these 6 wards were fed for 18 weeks by feeding the basic feed of Example 2 and a test feed containing 10% of BF and other various dietary fibers according to the procedure of Example.
In the period of 9 weeks from the start of breeding, 20 mg / kg body weight of 1,2-dimethylhydrazine was administered once a week using a gastric tube, and after 18 weeks, the rat was disassembled and tumor in the intestine developed. When the situation was investigated, the removal of BF poisoning performance was remarkable in the products summarized in Table 5.

1) GF, FF, PF and WF are dietary fibers prepared from burdock, wheat bran, pectin (citrus fruits) and wood cellulose, respectively.

2) Mean ± standard error (same below) Test Example 2 Three-week-old SD rat (male) was bred with the basic feed of Example 2 to about 100 g, and the body weight was standardized to 10 animals per group. 3 test zones were prepared. Rats in these three sections were fed and administered with the basic feed of Example 2, BF-added feed and 1,2-dimethylhydrazine in the manner summarized in Table 6, and after 18 weeks, the rat was disassembled to generate a tumor in the intestine. When the situation was investigated, the product summarized in Table 6 shows whether or not BF exists in the digestive organs of rats when 1,2-dimethylhydrazine is administered.
It was recognized that it is greatly involved in the removal of poisonous performance.

Front page continuation (72) Inventor Tsutomu Arizuka 13th west, 9th line, Inada-cho, Obihiro City, Hokkaido, Japan Research Institute for Sugar Beet Sugar Co., Ltd. (56) Reference Japanese Patent Publication 58-15108 (JP, B2) Japanese Patent Publication 4 -6686 (JP, B2)

Claims (1)

[Claims] 1. A mutagen poisoning performance removing agent (excluding digestion and absorption poisoning performance) containing as an active ingredient dietary fiber obtained from sugar beet pulp containing a composite fiber of water-soluble dietary fiber and insoluble fiber.