JPH06101994B2 - Digestion-resistant enteric useful bacteria and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention provides a digestion resistance that allows bacteria having a useful effect on the human body in the large intestine, such as the group of Bifidobacterium, to reach the large intestine without being inactivated in the gastrointestinal tract. The present invention relates to useful enteric bacteria and a method for producing the same.

[Conventional technology]

Bifidobacteria that are useful enteric bacteria are anaerobic bacteria,
Sensitive to oxygen, acids and humidity. Although the amount of the bifidobacteria group varies depending on race, environment, age, etc., it has been found that it is present in the jejunum, ileum, cecum, and rectum, especially in the large intestine. When the Bifidobacterium group of the large intestine is present in the large intestine, the bacterial flora in the large intestine is ideally maintained, the intestinal infection of the pathogenic bacterium is prevented, diarrhea is prevented and treated, bowel movement is eliminated, and adult diseases are prevented. It has effects such as enhancing immunity, and generally the amount of bifidobacteria tends to decrease as the individual ages.
(Michioka Chizu, Pharmaceutical Factory, VOL.6, No.5 (1986), P.424 ~ 48
8) In order to propagate a large amount of such Bifidobacteria in the large intestine, various methods of orally supplying from the outside with food have been studied. However, since all the conventional methods have administered bifidobacteria in the form of liquid, paste, powder, granules, tablets, etc., they are inactivated by the strongly acidic gastric juice produced by the gastric mucosa when passing through the stomach. However, Bifidobacterium had lost its efficacy before reaching the intestine.

[Problems to be Solved by the Invention]

When a group of orally administered bifidobacteria passes through the stomach, it protects the bifidobacteria so that they are not inactivated and reaches the intestine, especially the large intestine, where a large amount of bifidobacteria is released in the large intestine. It was being done.

[Means for solving problems]

The present invention is to solve the above-mentioned problems, and the composition thereof is such that useful enteric bacteria are filled in a capsule made of a film composed of a natural polysaccharide / polyhydric alcohol composition, and the capsule has a temperature of gastric temperature or higher. It is characterized by being coated with an edible oil / fat having a melting point.

Intestinal useful bacteria in the present invention is not limited to bifidobacteria, lactic acid bacteria, Streptococcus genus Feucaris, Lactobacillus acidophilus such as all those that keep good intestinal bacterial flora are included. It Among them, longum typified by Bifidobacteria is preferable.

Useful bacteria in the intestine are stored in capsules. Since the surface of the capsule is further covered with edible oil and fat, there is no risk of digestion in the stomach, but it is an edible substance having rigidity or strength that does not cause bag breakage in the stomach. Above all, a non-digestible material that is not digested even in the small intestine is preferable.

The capsule material in the present invention is disclosed in JP-A-60-267273, JP-A-63-28380 and JP-B-4-57305, and polyhydric alcohols, sugar alcohols, monosaccharides and disaccharides. And in at least one concentrated solution selected from oligosaccharides, carrageenan, carginic acid, alginic acid derivative, agar, locust bean gum, guar gum,
A natural polysaccharide / polysaccharide obtained by uniformly kneading at least one natural polysaccharide selected from tamarind seed polysaccharide, pectin, xanthan gum, glucomannan, chitin and pullulan in the presence or absence of an alkali. A polyhydric alcohol composition is used.

As a method for producing the capsule, for example, the capsule die may be dipped in a concentrated aqueous solution of the above material and dried, or the sheet produced in advance may be heated and compressed to be formed into a certain die.

The capsule is provided with a plurality of fine holes. The size of the pores varies depending on the type and thickness of the material, but it is the size that allows bacteria to freely pass, and is 0.1 to 5μ, preferably 0.5 to 3μ.
Is. When forming the holes, a device that allows the needles to freely project or store a plurality of needle-shaped objects in the mold of the capsule is provided so that they are projected when the capsule is molded and stored when the mold is removed. Multiple holes can be provided at the same time. Alternatively, holes may be provided after capsule formation, and there are also a mechanical method using a hot needle and a method using a laser beam. Furthermore, a small amount of fine particles made of a digestible material may be added to the natural polysaccharide / polyhydric alcohol composition of the present invention to form a capsule. In this case, the digestible material is digested and extinguished in the small intestine, and then a plurality of holes are provided. By adopting this method, the contents can be released relatively early from the end of the small intestine.

Further, even if no pores are provided, the capsule material of the present invention swells with water and is rubbed, whereby cracks and perforations naturally occur, and intestinal useful bacteria can be reliably released in the large intestine.

Furthermore, in order to prevent gastric juice from penetrating into the capsule during passage through the stomach, the outer surface of the capsule is densely coated with edible oil and fat. Edible fats and oils need to have a melting temperature that does not fall off due to the temperature in the stomach, and are 35 ° C or higher, preferably 45 to 60 ° C. In reality, hardened edible fats, beef tallow, lard and the like are preferably used.

In this way the capsule reaches the intestine protected from direct contact with gastric juice. In the intestines, oils and fats are digested by the action of gastric reversal, intestinal revertase, pancreatic revertase, bile, etc., and the capsule is exposed. If the capsule is a digestive material such as protein or digestive polysaccharide, all Bifidobacteria are released in the small intestine. Since the small intestine has a strong digestive action and is not a favorable environment for Bifidobacterium, it is preferable to minimize the release in the small intestine and reach the large intestine. Since the present invention uses a non-digestible and bacteria-permeable capsule material, it is possible to continuously release bifidobacteria from the small intestine to the large intestine, and to allow a large number of bifidobacteria to reach the large intestine.

In order for the bifidobacteria that have reached the large intestine to grow further,
It is preferable that the capsule coated with edible oil and fat is coated with a seasoning substance containing fructooligosaccharide, which is a growth-promoting element of bifidobacteria, as a main component. Fructooligosaccharide has a structure in which 1 molecule of fructose is bound to 1 molecule of sucrose and is also used as a low-calorie sweetener.

[Action]

The present invention stores a group of bifidobacteria in a capsule composed of an edible and non-digestible natural polysaccharide / polyhydric alcohol composition, and coats it with an edible oil that does not dissolve at gastric temperature to protect it from gastric acid. It is a thing. After reaching the small intestine, the oils and fats are digested and the capsules are exposed. At this time, because the capsule is a non-digestible material that allows bacteria to pass through,
Since the form of the capsule does not disintegrate even when reaching the small intestine, Bifidobacterium is gradually released from the pores, reaches the large intestine, and continues to be released. Therefore, it becomes possible to orally allow a large amount of bifidobacteria to reach the large intestine and ideally maintain the intestinal bacterial flora. Furthermore, when the edible oil / fat is coated with fructooligosaccharides, it not only has a pleasant mouthfeel upon ingestion, but is also a growth factor for the Bifidobacterium group after reaching the intestine, further activating the Bifidobacterium.

〔Example〕

Refer to Hidenobu Mashima, Physiology, Bunkodo edition, pp. 418-446 for the release test of Bifidobacteria in the following examples. That is, the elapsed time of the test was as follows: stomach 30 minutes, small intestine (duodenum to cecum) 4 hours, large intestine (cecum to rectum) 13
Time was 30 minutes. In the examples, the test time was roughly divided into two parts, that is, the stomach part was 30 minutes and the intestine part was 17 hours and 30 minutes, for a total of 18 hours.

Test liquid is Japanese Pharmacopoeia 10, General Test Method, page 734, No. 1
A liquid (pH about 1.2 ... corresponding to gastric juice) and a second liquid (pH about 6.8 ... corresponding to intestinal fluid) were used, and lipase was added for the purpose of dissolving the edible oil / fat film.

After the test, to confirm the bifidobacteria released in the second liquid, a certain amount of the test liquid after the test was sprayed on the BL agar medium,
Anaerobic culture was carried out, and after the lapse of a predetermined culture time, the presence and number of colonies were measured.

Example 1 60 parts by weight of carrageenan, 20 parts by weight of alginic acid, 10 parts by weight of locust bean gum, and 10 parts by weight of glucomannan were kneaded, and 30 parts by weight of glycerin was added thereto and mixed well to give a squeaky powdery natural substance. A polysaccharide / polyhydric alcohol composition was obtained.

3 parts by weight of this composition was dissolved in 97 parts by weight of water to make a viscous solution, and a plurality of needles were freely operated by remote control to project the solution, and the capsule type was used to store an average film thickness of 50-100 μm.
Capsules were manufactured. This capsule is heat resistant,
The size of the pores was 0.5-3μ. 0.5 g of Bifidobacteria longum (10 ⁸ pieces / g) was inserted into this capsule, and the end of the capsule was heat-sealed and sealed. Next, melting point 40 ℃ ± 2
Hardened edible oils and fats (shortening refined beef oil, manufactured by Yokozeki Yushi Kogyo Co., Ltd.) at ℃ were coated.

The capsule was immersed in 50 ml of the first liquid at 37 ° C. for 30 minutes, and then in 250 ml of the second liquid for 17 hours and 30 minutes. To the second liquid, 0.1 g of commercially available lipase (manufactured by Amano Pharmaceutical Co., Ltd.) was added. After the immersion, dip the immersion liquid to various magnifications and collect 1 ml.
After inoculating to L agar medium (manufactured by Eiken Co., Ltd.), anaerobic culture was performed at 37 ° C. for 72 hours, and the number of colonies was measured. The number of colonies is the average number of 3 petri dishes.

Unmeasurable number with 10,000-fold dilution, 80 with 100,000-fold dilution,
Five colonies were confirmed with a 1,000,000-fold dilution.

Example 2 A test was carried out in the same manner as in Example 1 except that a hardened edible oil / fat having a melting point of 40 ° C. ± 5 ° C. (refined edible oil / fat, manufactured by Yokozeki Yushi Kogyo Co., Ltd.) was coated. As a result, an unmeasurable number was confirmed at 10,000-fold dilution, 50 colonies at 100,000-fold dilution, and 8 colonies at 1,000,000-fold dilution.

Comparative Example 1 A test was conducted in the same manner as in Example 1 except that the capsule was not coated with fat and oil and that lipase was not added to the second liquid. As a result, unmeasurable numbers were confirmed at 10-fold dilution, 50 at 100-fold dilution, 12 at 1000-fold dilution, and 0 at 10,000-fold dilution.

Comparative Example 2 According to the conventional technique, when the test of Example 1 was carried out as it is without coating and protecting Bifidobacteria, it was completely inactivated by gastric juice and no colonies existed.

〔The invention's effect〕

INDUSTRIAL APPLICABILITY According to the present invention, it becomes possible to allow useful intestinal bacteria to reach the intestine without being inactivated by gastric juice, and it becomes easy to always keep the intestinal bacterial flora normal.

Claims (6)

[Claims] 1. A polyhydric alcohol, a sugar alcohol, a monosaccharide,
In at least one stock solution or concentrated solution selected from disaccharides and oligosaccharides, glucomannan, carrageenan, alginic acid, alginic acid derivative, agar, locust bean gum, guar gum, tamarind seed polysaccharide, pectin,
From a powdery natural polysaccharide / polyhydric alcohol composition obtained by uniformly mixing at least one natural polysaccharide selected from xanthan gum, chitin and pullulan in the presence or absence of an alkali. A digestive-resistant enteric useful bacterium characterized in that a digestive-resistant enteric useful bacterium is encapsulated in a non-digestible film capsule, and the capsule is coated with an edible oil having a melting point of gastric temperature or higher. . 2. The digestive-resistant intestinal useful bacterium according to claim 1, wherein the capsule has micropores. 3. The digestive-resistant intestinal useful bacterium according to claim 1 or 2, wherein the intestinal useful bacterium is a group of bifidobacteria. 4. The digestive-resistant intestinal useful bacterium according to claim 1 or 3, wherein the fine particles digested in the small intestine are blended in a non-digestible film. 5. A polyhydric alcohol, a sugar alcohol, a monosaccharide,
In at least one stock solution or concentrated solution selected from disaccharides and oligosaccharides, glucomannan, carrageenan, alginic acid, alginic acid derivative, agar, locust bean gum, guar gum, tamarind seed polysaccharide, pectin,
A powdery natural polysaccharide / polyhydric alcohol composition obtained by uniformly mixing at least one natural polysaccharide selected from xanthan gum, chitin and pullulan in the presence or absence of an alkali. A method for producing a digestive-resistant enteric useful bacterium, which comprises molding a capsule using an aqueous solution, and then coating the capsule with an edible oil / fat having a melting point of gastric temperature or higher. 6. The digestive-resistant intestinal tract according to claim 5, characterized in that the capsules are provided with fine pores at the same time as or after the capsule molding and then covered with edible oil and fat. How to make useful bacteria.