JPH0621858B2 - Light irradiation mechanism such as particle counter - Google Patents
Light irradiation mechanism such as particle counterInfo
- Publication number
- JPH0621858B2 JPH0621858B2 JP59086785A JP8678584A JPH0621858B2 JP H0621858 B2 JPH0621858 B2 JP H0621858B2 JP 59086785 A JP59086785 A JP 59086785A JP 8678584 A JP8678584 A JP 8678584A JP H0621858 B2 JPH0621858 B2 JP H0621858B2
- Authority
- JP
- Japan
- Prior art keywords
- light
- flow cell
- optical fiber
- irradiation mechanism
- slit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
【発明の詳細な説明】 （イ）目的 （産業上の利用分野） 本発明は、フローセル中を通過する血液細胞等の如き粒
子を計数する粒子計数装置、その細胞を分類する細胞分
類装置、あるいはその細胞を分離する細胞分離装置など
で使用され、フローセル中を通過する粒子に光を照射す
るための光照射機構に関するものである。DETAILED DESCRIPTION OF THE INVENTION (a) Purpose (Field of Industrial Application) The present invention relates to a particle counting device for counting particles such as blood cells passing through a flow cell, a cell sorting device for sorting the cells, or The present invention relates to a light irradiation mechanism for irradiating particles passing through a flow cell with light, which is used in a cell separation device for separating the cells.
（従来の技術） 上記の如き粒子計数装置等では細孔をもつフローセル中
に細胞などの粒子を含むサンプルを流し、そのサンプル
の流れの側方から光を照射してそのサンプル中の粒子に
よる光の散乱光又は蛍光を検出することにより、その粒
子を計数したり、分類したりする。そのため、サンプル
に照射される光は、サンプル流の直径にわたってサンプ
ル流を横切り、流れの方向に薄いスリット状の光束であ
ることが必要である。(Prior Art) In a particle counting device such as the one described above, a sample containing particles such as cells is made to flow in a flow cell having pores, and light from the side of the flow of the sample is radiated to emit light by the particles in the sample. The particles are counted or classified by detecting the scattered light or fluorescence of. Therefore, the light with which the sample is irradiated needs to be a thin slit-like light beam that traverses the sample flow over the diameter of the sample flow and is thin in the flow direction.
従来、このような薄いスリット状の光束を得るために、
第２図に示されるような光学系が使用されている。１は
軸方向にみたフローセルで、中央の細孔２をサンプルが
図で紙面垂直方向に流れる。サンプルを照射する光束
は、レンズ３，４を用いてスリット５の像をフローセル
１中に結像させることにより得ている。６はそのスリッ
ト５の像、７は光源である。Conventionally, in order to obtain such a thin slit-like luminous flux,
An optical system as shown in FIG. 2 is used. Reference numeral 1 is a flow cell as viewed in the axial direction, and the sample flows through the central pore 2 in the direction perpendicular to the plane of the drawing. The light flux that illuminates the sample is obtained by forming the image of the slit 5 in the flow cell 1 using the lenses 3 and 4. 6 is an image of the slit 5, and 7 is a light source.
（発明が解決しようとする問題点） スリットの像を結像させる従来のこのような光照射機構
では、結像用のレンズが必要となり、そのレンズ系の焦
点位置や光軸を調整するのが非常に困難であるという問
題がある。(Problems to be Solved by the Invention) In such a conventional light irradiation mechanism for forming an image of a slit, a lens for image formation is required, and it is necessary to adjust the focal position and optical axis of the lens system. The problem is that it is very difficult.
本発明は、フローセルのまわりの光学系が簡単で、小形
になり、またメンテナンスも容易な光照射機構を提供す
ることを目的とするものである。It is an object of the present invention to provide a light irradiation mechanism in which an optical system around a flow cell is simple, compact, and easy to maintain.
（ロ）発明の構成 （問題点を解決するための手段） 本発明は、粒子計数装置等の光照射機構であって、一方
の端面がほぼ円形に束ねられ、他方の端面がフローセル
の細孔の直径にわたる長さのスリット状に配列されてそ
のフローセルの細孔の近くに埋設された光ファィバ束
と、 光源及びその光源の光を前記光ファィバの束の円形端面
に導く光学系と、を備えて構成されたものである。(B) Configuration of the Invention (Means for Solving Problems) The present invention is a light irradiation mechanism such as a particle counting device, in which one end surface is bundled into a substantially circular shape and the other end surface is a pore of a flow cell. An optical fiber bundle which is arranged in a slit shape having a length extending over the diameter of and is embedded near the pores of the flow cell, and a light source and an optical system which guides the light of the light source to the circular end surface of the bundle of optical fibers. It is prepared for this purpose.
本発明では、光源から光学系により導かれた光束を光フ
ァイバ束によりフローセルの細孔の近くに導き、かつ、
その導かれた光束の形状は、フローセル細孔側の光ファ
イバ束の端面がスリット状になるように光ファイバ束が
配列されることによりスリット状に形成される。In the present invention, the light flux guided by the optical system from the light source is guided near the pores of the flow cell by the optical fiber bundle, and,
The shape of the guided light flux is formed into a slit shape by arranging the optical fiber bundles so that the end surfaces of the optical fiber bundles on the side of the flow cell pores are slit-shaped.
（実施例） 第１図は本発明の一実施例を表わす。(Embodiment) FIG. 1 shows an embodiment of the present invention.
１０は光ファイバケーブルであり、複数の光ファイバ１
１を樹脂などによりモールドしたものである。この光フ
ァイバケーブル１０の一方の端面ではその光ファイバ１
１の束がほぼ円形になるように束ねられており、他方の
端面ではその光ファイバ１１の束がフローセル１の細孔
２の直径にわたる長さのスリット状になるように一列に
配列されている。そして、スリット状の光ファイバ束を
もつ端部は、フローセル１の細孔２にできるだけ近づけ
られて、スリット状の光ファイバ束の配列方向がその細
孔２の軸方向に直交するように位置決めされている。10 is an optical fiber cable, and a plurality of optical fibers 1
1 is molded with resin or the like. At one end face of the optical fiber cable 10, the optical fiber 1
One bundle is bundled so as to have a substantially circular shape, and on the other end face, the bundle of optical fibers 11 is arranged in a line so as to form a slit shape having a length extending over the diameter of the pore 2 of the flow cell 1. . Then, the end portion having the slit-shaped optical fiber bundle is brought as close as possible to the pore 2 of the flow cell 1 and positioned so that the arrangement direction of the slit-shaped optical fiber bundle is orthogonal to the axial direction of the pore 2. ing.
光ファイバケーブル１０の円形状光ファイバ束をもつ端
面側には、図には示されていないが光源と、その光源か
らの光をその端面に導く光学系が設けられている。Although not shown in the figure, a light source and an optical system for guiding light from the light source to the end surface are provided on the end surface side of the optical fiber cable 10 having the circular optical fiber bundle.
本実施例において、光源及び光学系から光ファイバケー
ブルの一方の端面に光１２を照射すれば、その光束は光
ファイバ１１中を通って他方の端面でスリット状に広げ
られ、フローセル１の細孔２を横切る薄い光束１３とな
って細孔２を照射する。そこで、フローセル１の細孔２
中を矢印のようにサンプル液１４を流すと、その中の粒
子が光ファイバ束からの光束１３を横切る時にその光束
１３をさえぎるので、その光束１３の透過光、散乱光又
は蛍光を検出することにより、粒子数を計数したり、散
乱光の強度などからその通過粒子の大きさや形状を判別
し、粒子の種類を判定することもできる。In this embodiment, when light 12 is emitted from the light source and the optical system to one end face of the optical fiber cable, the light flux passes through the optical fiber 11 and is spread in a slit shape on the other end face, and the pores of the flow cell 1 are formed. A thin light beam 13 that crosses 2 is irradiated onto the pore 2. Therefore, the pores 2 of the flow cell 1
When the sample liquid 14 is flown through the inside as shown by an arrow, when the particles in the sample liquid 14 cross the light flux 13 from the optical fiber bundle, the light flux 13 is blocked, so that transmitted light, scattered light or fluorescence of the light flux 13 should be detected. Thus, the number of particles can be counted, and the size and shape of the passing particles can be determined from the intensity of scattered light and the like to determine the type of particles.
本実施例において、フローセル１に埋設された光ファイ
バケーブル１０の端面が細孔２に近ければ近いほど細孔
２での光ファイバ１１の端面からの光の拡散が小さく、
薄い光束となる。そのため、粒子がその光束を通過する
瞬間だけその光束が遮ぎられることになるので、より正
確な計数、あるいは分類ができるようになる。In this example, the closer the end surface of the optical fiber cable 10 embedded in the flow cell 1 is to the pore 2, the smaller the diffusion of light from the end surface of the optical fiber 11 in the pore 2,
It becomes a thin luminous flux. Therefore, since the light flux is blocked only at the moment when the particles pass through the light flux, more accurate counting or classification can be performed.
本発明の光照射機構を用いて、散乱光によりサンプル中
の粒子を計数したり、分類や分離を行なう方法の例を第
３図及び第４図に示す。FIGS. 3 and 4 show an example of a method of counting, classifying and separating particles in a sample by scattered light using the light irradiation mechanism of the present invention.
第３図は照射光１３と直交する方向の散乱光を検出する
方式であり、フローセル１の軸方向からみた図である。
照射光１３と直交する方向にレンズ系２０，２１と検出
器２２を配置しておくと、フローセル１中を粒子が通過
したときだけその検出器２２方向に散乱光２３が現れる
ので、それにより粒子数やその粒子の種類を判別するこ
とができる。FIG. 3 shows a method of detecting scattered light in a direction orthogonal to the irradiation light 13 and is a view seen from the axial direction of the flow cell 1.
When the lens systems 20 and 21 and the detector 22 are arranged in the direction orthogonal to the irradiation light 13, the scattered light 23 appears in the direction of the detector 22 only when the particle passes through the flow cell 1, so that the particle It is possible to determine the number and the type of the particles.
検出器２２は光電子増倍管や半導体光検出素子など、種
々のものを使用することができる。第４図は照射光１３
の方向にレンズ系２０，２１と検出器２２を配置した例
であり、フローセル１の上方からみた図である。このと
き、フローセル１中を粒子が通過しないときの照射光１
３がレンズ系の遮光板２４でさえぎられて検出器２２に
到達しないようにしておく。そして、フローセル１中を
粒子が通過すると、散乱光が生じ、この散乱光は遮光板
２４の外側を通って検出器２２で検出される。As the detector 22, various ones such as a photomultiplier tube and a semiconductor photodetector can be used. FIG. 4 shows irradiation light 13
It is an example in which the lens systems 20 and 21 and the detector 22 are arranged in the direction of, and is a view seen from above the flow cell 1. At this time, irradiation light 1 when particles do not pass through the flow cell 1
3 is prevented from reaching the detector 22 by being blocked by the light shielding plate 24 of the lens system. Then, when the particles pass through the flow cell 1, scattered light is generated, and this scattered light passes through the outside of the light shielding plate 24 and is detected by the detector 22.
（ハ）発明の効果 以上のように、本発明の光照射機構は光ファイバにより
光をフローセルの細孔の近くまで導き、かつスリット状
に整形してサンプル流を照射するようになっているの
で、従来のようにレンズを組み合せた光照射機構と比較
すると、次のような優れた点を備えている。(C) Effects of the Invention As described above, the light irradiation mechanism of the present invention guides light to the vicinity of the pores of the flow cell by the optical fiber and shapes the light into a slit to irradiate the sample flow. Compared with the conventional light irradiation mechanism in which a lens is combined, it has the following excellent points.
（１）フローセルのまわりの光学系が簡単になり、かつ
小形化される。(1) The optical system around the flow cell is simplified and miniaturized.
（２）ファイバケーブルで光を導くので、フローセルと
光源の相対位置を自由に設定することができる。(2) Since the light is guided by the fiber cable, the relative positions of the flow cell and the light source can be freely set.
（３）従来のような光学系の焦点位置の調整が不要にな
る。(3) There is no need to adjust the focus position of the optical system as in the conventional case.
（４）使用中に焦点位置や光軸の狂いが生じてこない。(4) The focus position and the optical axis are not displaced during use.
（５）メンテナンスが容易になる。(5) Maintenance becomes easy.
（６）従来は実際にサンプルに照射される光束は光源か
らの光束のうちでスリットを通過した一部の光束のみで
あったので、無駄が多い欠点もあったが、本発明では光
源の光束を光ファイバ束の一端で受け、光ファイバ束で
導いて他端でスリット状に整形するものであるので、光
源の光束を有効に利用することができる。(6) Conventionally, the light flux actually applied to the sample is only a part of the light flux from the light source that has passed through the slit, so that there is a waste of many light fluxes. Is received at one end of the optical fiber bundle, guided by the optical fiber bundle, and shaped into a slit at the other end, so that the luminous flux of the light source can be effectively used.
第１図は本発明の一実施例を示す斜視図、第２図は従来
の光照射機構を示す概略図、第３図及び第４図はそれぞ
れ本発明の光照射機構を用いた粒子計数装置等の例を示
す概略図である。 １……フローセル、２……フローセルの細孔、１１……
光ファイバ束。FIG. 1 is a perspective view showing an embodiment of the present invention, FIG. 2 is a schematic view showing a conventional light irradiation mechanism, and FIGS. 3 and 4 are particle counting devices using the light irradiation mechanism of the present invention. It is a schematic diagram showing an example of etc. 1 ... Flow cell, 2 ... Flow cell pores, 11 ...
Fiber optic bundle.
Claims (1)
端面がフローセルの細孔の直径にわたる長さのスリット
状に配列されてそのフローセルの細孔の近くに埋設され
た光ファィバ束と、 光源及びその光源の光を前記光ファィバ束の円形端面に
導く光学系と、を備えたことを特徴とする粒子計数装置
等の光照射機構。1. An optical fiber bundle in which one end surface is bundled into a substantially circular shape, and the other end surface is arranged in a slit shape having a length extending over a diameter of a pore of a flow cell and embedded near the pore of the flow cell. And a light source and an optical system for guiding the light of the light source to the circular end surface of the light fiber bundle, and a light irradiation mechanism such as a particle counting device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59086785A JPH0621858B2 (en) | 1984-04-28 | 1984-04-28 | Light irradiation mechanism such as particle counter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59086785A JPH0621858B2 (en) | 1984-04-28 | 1984-04-28 | Light irradiation mechanism such as particle counter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60231134A JPS60231134A (en) | 1985-11-16 |
| JPH0621858B2 true JPH0621858B2 (en) | 1994-03-23 |
Family
ID=13896406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59086785A Expired - Lifetime JPH0621858B2 (en) | 1984-04-28 | 1984-04-28 | Light irradiation mechanism such as particle counter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0621858B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03148041A (en) * | 1989-11-02 | 1991-06-24 | Hamamatsu Photonics Kk | Particle counter used in liquid |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5537886Y2 (en) * | 1975-03-07 | 1980-09-04 | ||
| JPS5766342A (en) * | 1980-04-28 | 1982-04-22 | Agency Of Ind Science & Technol | Optical measuring method for suspension particles in medium |
-
1984
- 1984-04-28 JP JP59086785A patent/JPH0621858B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60231134A (en) | 1985-11-16 |
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