JPH0621982B2 - Three-dimensional spectrum display device - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to a three-dimensional spectrum display device, and more particularly to a three-dimensional spectrum display device suitable for quantitatively handling a spectrum having two parameters.

[Background of the Invention]

Some spectra are defined as a function of two parameters (factors). For example, excitation wavelength and fluorescence wavelength of fluorescence spectrum, retention time and wavelength of chromatograph. These have three-dimensional information including the spectrum intensity, and are displayed in a map form by contour lines.

This spectral display method is described in "Total Luminescence Spectrosco by Suter et al. In Chimia. 37. (1983).
It is discussed in the literature entitled "py".

The above-mentioned conventional technique uses a three-dimensional spectrum (here, the three-dimensional spectrum is a so-called three-dimensional spectrum in which the spectrum is displayed in a coordinate system of _two factors P ₁ and P _{2 which} are the factors that determine the spectrum.
It is one of the display methods on a two-dimensional plane (which means spectrum display based on three-dimensional information), but as another method that has been performed before, a three-dimensional drawing of spectra as mountains and valleys in a three-dimensional space. There is something to display by law. This latter display method is suitable for listing the irregularities of the spectrum, but if there is a mountain of the spectrum in the foreground, the shaded part will not be displayed, and there will be information leakage and Has the drawback of being difficult to read quantitatively.

On the other hand, the former contour line display method displays peaks and valleys of a three-dimensional spectrum as contour lines on a two-dimensional plane.
Although all spectrum information can be displayed, there is still a problem that it is difficult to read the spectrum intensity quantitatively. Usually, in spectrum analysis, the position information (wavelength, time, etc.) of the spectrum peak provides information for the characteristics and identification of the measured substance, and the peak intensity provides information about the concentration or abundance of the measured substance. Therefore, lack of quantification is often a fatal drawback.

[Object of the Invention]

The present invention has been made in view of the above points, and an object thereof is to provide a three-dimensional spectrum display device which can effectively display a three-dimensional spectrum having two factors in a two-dimensional space and can be quantitatively handled. It is in.

[Outline of Invention]

The present invention, spectral two factors P _1, one of the factors P ₁ and scan the other factor P ₂ in a fixed state the factor P ₂ scanning a predetermined unit of factor P ₁ a of P ₂ which is a determinant of A storage device for storing a large number of spectrum signals obtained by executing the calculation every time in step 3 together with the factors P ₁ and P ₂ as three-dimensional information, and a point having the same spectrum intensity from the stored three-dimensional information. A three-dimensional spectrum display device comprising: an image display device which extracts and collects a set of those points on the screen as a contour line of spectral intensity in the coordinate system of the factors P ₁ and P ₂ ; Cursor control means for designating any two points of the coordinate system of the contour line displayed on the screen by the cursor, and spectral intensity of the coordinates on the line between the designated two points from the contour line. Wherein the storage device reads two factors P _1, factor in relation to the one of the P ₂ P ₁ - spectral intensity or factor P ₂ - Create a 2-dimensional spectrum graph of the spectral intensity of the image display device screen And a two-dimensional graph creating means for displaying through.

According to the above configuration, when the spectrum signal determined by the _two factors P ₁ and P _{2 is} displayed on the screen on a two-dimensional plane, the spectrum intensity is displayed in contour lines in the factor P ₁ and P ₂ coordinate systems, and cursor control is performed. By means of the above-mentioned contour lines P ₁ ,
If you specify any two points in the P ₂ coordinate system with the cursor,
The spectrum intensity of the coordinates on the line between the two designated points out of the contour lines is read from the storage device of the three-dimensional information (factors P ₁ , P ₂ , spectrum), and based on the read information, 2 The dimensional graph creating means creates a two-dimensional spectrum graph of factor P ₁ -spectral intensity or factor P ₂ -spectral intensity, and this two-dimensional spectrum graph is displayed on the screen through the image display device.

Example of Invention

 Embodiments of the present invention will be described with reference to the drawings.

FIG. 1 is a block diagram of a three-dimensional spectrum display device according to an embodiment of the present invention.

In FIG. 1, reference numeral 1 is a spectrofluorometer having two spectroscopes for excitation and fluorescence, and 2 is a spectrum signal from the spectrofluorometer 1 to store spectrum information at an address corresponding to a wavelength. A spectrum storage device 3, a CRT display as an image display device, and a cursor control device 4 which can designate any two points on the screen of the CRT display.

The spectrofluorometer 1 first scans the fluorescence wavelength (factor P ₂ ) with the excitation wavelength (factor P ₁ ) fixed, and sends the spectrum signal at that time to the spectrum storage device 2 at regular wavelength intervals.

The spectrum storage device 2 reads the excitation wavelength and the fluorescence wavelength of the spectrofluorometer 1, and stores the spectrum signal at the address corresponding to them as three-dimensional information (spectrum signal in which the excitation wavelength and the fluorescence wavelength are combined). . That is, the spectrofluorometer 1 returns the fluorescence wavelength to the start wavelength when the fluorescence wavelength scanning is completed, drives the excitation wavelength by a fixed amount (predetermined unit), and then performs the fluorescence wavelength scanning. At the start, the fluorescence spectrum signal is stored in the spectrum storage device 2 together with the excitation wavelength and the fluorescence wavelength as three-dimensional information.

By repeating this operation until the excitation wavelength reaches the final wavelength, all three-dimensional information (excitation wavelength, fluorescence wavelength, spectrum signal) is stored in the spectrum storage device 2.

Thereafter, the calculation means (three-dimensional spectrum generation means) in the image display device 3 reads the stored information in the spectrum storage device 2, extracts the excitation wavelength and the fluorescence wavelength at which the spectrum intensity is equal to a certain value, and extracts them. Plot the set of points on a screen (coordinate system) with the fluorescence wavelength (λ _EM ) as the horizontal axis and the excitation wavelength (λ _EX ) as the vertical axis. By performing this for all spectrum signals stored in the spectrum storage device 2, as shown in FIG.
The contour map 5 of the spectrum, that is, a so-called three-dimensional spectrum is obtained on the screen. As the spectral intensity for contour line extraction, some discrete values are selected, and a plurality of contour lines are displayed according to the number of points.

An example of the above procedure is shown in the flowchart of FIG.

The cursor control device 4 of FIG. 1 designates two arbitrary points for crossing the contour map 5 by moving the cursor on the image display device 3.
As shown in FIGS. 4 and 5, the two cursors 9 and 10 are used to designate the above-mentioned two points.

The calculation means of the image display device 3 calculates the corresponding excitation wavelength and fluorescence wavelength on a straight line between the two points connecting the cursors 9 and 10, and further, the spectrum signal corresponding to the excitation wavelength and fluorescence wavelength. Is read from the spectrum storage device 2, a two-dimensional spectrum graph having the excitation wavelength on the horizontal axis and the spectrum intensity on the vertical axis is created and displayed on the screen of the image display device 3. That is, the arithmetic system of the image display device 3 constitutes a two-dimensional graph creating means in addition to the above-mentioned three-dimensional spectrum creating means.

FIG. 5 is a diagram showing an example of the two-dimensional spectrum graph. FIG. 6 is a flow chart showing an embodiment of the procedure at that time.

In FIG. 6, the coordinates (Xc ₁ , Yc ₁ ) and (Xc ₂ , Yc ₂ ) of two points that cross the contour line 5 by the cursors 9 and 10 are shown.
Is specified, in order to read the spectrum data on the line between the three-dimensional spectrum graphs (contour lines), Xc ₁ ... X _i
... adding increments predetermined unit ΔX to X _i in the range of Xc _2, for Y-coordinate, the range of Yc ₁ ... Y _i ... Yc _2, from the coordinates of the slope of the two points, The spectral intensity I _XM at each coordinate (X _i , Y _i ) on the line connecting the two points is obtained by the formula (1) and the point corresponding to the spectral data I _XM with respect to X _i (excitation wavelength) is plotted to obtain the excitation wavelength − Two-dimensional spectral data of spectral intensity is created.

3 and 4, a contour map having two peaks is shown. In such a case, a straight line connecting two points designated by the two cursors 9 and 10 is as shown in the figure. It is desirable to configure the cursor control device 4 so as to specify two points so as to pass through two peaks at the same time. By doing so, a graph can be displayed as shown in FIG.

According to this embodiment, the measured spectrum can be displayed in the coordinate system of the excitation wavelength and the fluorescence wavelength without any omission by the contour map, and by converting it into the two-dimensional spectrum graph, the correspondence with the conventional two-dimensional spectrum can be obtained. In addition to facilitating the understanding of the characteristics and identification of the substance to be measured, it is also possible to facilitate quantitative treatment of the component concentration and abundance.

In the above example, the fluorescence spectrum is taken as an example, and the parameters are the excitation wavelength and the fluorescence wavelength.
Other parameters such as a combination of chromatographic retention time and wavelength may be used. Further, the spectrum storage device 2, the image display device 3, and the cursor control device 4 can be easily realized by software of a computer, and thus may be replaced with programs.

FIG. 7 and FIG. 8 show an example of the related art of the present invention.

In this example, the cursor control device 4 similar to that shown in FIG. 1 moves one cursor 11 on the image display device 3 to specify an arbitrary point on the contour map 5. As a result, the image display device 3 calculates the excitation wavelength and the fluorescence wavelength corresponding to the cursor position, further reads the spectrum signal corresponding to the excitation wavelength and the fluorescence wavelength from the spectrum storage device 2, and obtains those values. Image display device 3
Displayed on the screen of. Reference numerals 6, 7 and 8 in FIG. 7 indicate the excitation wavelength, the fluorescence wavelength and the fluorescence spectrum intensity, respectively. The above procedure is shown in the flowchart of FIG.

In this example, the two-dimensional spectrum data of the substance to be measured as in the above-described examples of the present invention cannot be obtained, but the spectrum of any one point can be accurately read.

〔The invention's effect〕

According to the present invention, a three-dimensional spectrum having _two factors P ₁ and P ₂ is displayed in a two-dimensional space by contour lines to enable full spectrum display without information leakage, and the three-dimensional spectrum is a factor. By converting it into a two-dimensional spectrum graph of P ₁ -spectral intensity or factor P ₂ -spectral intensity, it becomes easy to grasp the position information and intensity of the spectral peak, and the characteristics, identification and quantitativeness (concentration, abundance) of the substance Etc.) can be provided.

[Brief description of drawings]

FIG. 1 is a block configuration diagram of a three-dimensional spectrum display device according to an embodiment of the present invention, FIG. 2 is a flowchart showing an example of a procedure for displaying spectrum contour lines in FIG. 1,
FIG. 3 is an explanatory view showing an example of a screen of the screen display device used in the present embodiment, FIG. 4 is an explanatory view in which only contour lines are taken out from FIG. 3, and FIG. 5 is the above three-dimensional spectrum (contour lines).
A diagram showing a state in which is converted into a two-dimensional spectrum graph,
FIG. 6 is a flowchart showing an example of the procedure at that time,
FIG. 7 is an explanatory view of a screen display device showing another application example, and FIG.
FIG. 7 is a flow chart showing an embodiment of the spectrum signal reading procedure in FIG. 1 ... Spectrofluorometer, 2 ... Spectrum storage device, 3
...... Image display device (three-dimensional spectrum creating means, two-dimensional graph creating means), 4 ... Cursor control device, 5 ... Contour map, 6 ... Excitation wavelength (factor P ₁ ), 7 ... Fluorescent wavelength ... (Factor P ₂ ), 8 ... Fluorescent spectrum intensity, 9,
10 ... Cursor.

Claims (1)

[Claims] 1. Two factors P which are the determinants of the spectrum.
A large number of spectral signals obtained by scanning one factor P ₂ with _one factor P ₁ fixed among ₁ and P ₂ and executing the factor P ₂ scan every time the factor P ₁ is changed by a predetermined unit. With the factors P ₁ and P ₂ as three-dimensional information, and a point having the same spectral intensity is extracted from the stored three-dimensional information, and the set of these points is used as the factor P ₁ , 3-D spectrum screen as contour lines of spectral intensity in the coordinate system of P ₂ (and here 3D spectrum, the means to display the spectrum together with factors P _1, P ₂₎ and an image display device for displaying In a provided three-dimensional spectrum display device, cursor control means for designating any two points of the coordinate system of the contour line displayed on the screen of the image display device by a cursor, and the designated two points from the contour line. The spectrum intensity of the coordinates on the line between them is read out from the storage device, and the two-dimensional spectrum of the factor P ₁ -spectral intensity or the factor P ₂ -spectral intensity in relation to either one of the two factors P ₁ and P _2. A three-dimensional spectrum display device, comprising: a two-dimensional graph creation means for creating a graph and displaying it on the screen of the image display device.