JPH0635979B2 - Method and device for removing cleaning solution of reaction beads in inspection of blood, etc. - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention allows the coated antibody to react with a sample and a reagent in the test of blood and the like using reaction beads coated with antibody on the surface (reaction beads). After washing the reaction beads, the washing liquid removal method and the washing liquid removal device for removing the remaining washing liquid adhering to the inner wall surface of the test tube and the peripheral surface of the reaction beads when washing with the washing liquid in the test tube Regarding improvement.

[Prior Art] Washing of reaction beads after the reaction, which is performed in the inspection of blood or the like using reaction beads coated with an antibody, is performed by washing off the specimen or reagent that has come into contact with the reaction beads to check the change status of the antibody. It is for measurement, and it is desirable to completely remove the cleaning liquid used for cleaning in order to make the measured value accurate.

However, if this washing solution is removed by drying, the washing solution is dried while it remains attached to the surface of the reaction beads, and the reaction beads are then measured by a meter such as a colorimeter. Since an error occurs when measuring the change, the drying means cannot be used and is removed by suction.

As a means for this, as shown in FIG. 1, a suction tube a provided in a dispensing head that moves up and down with respect to the test tube T containing the reaction beads R and B is inserted into the test tube T. At this time, the suction pipe a is arranged so as to be positioned on one side in the pipe and is provided so as to hang longer than the dispensing pipe b for dispensing the washing liquid, and the lower end side of the suction pipe a can be flexibly bent. When the suction tube a is inserted into the test tube T after being formed in the flexible tube c, the flexible tube c on the lower end side bends following the inner surface of the tube wall of the test tube T to form an arc shape. There is a means for sucking and removing all the cleaning liquid remaining at the bottom by entering the bottom surface of the reaction beads R and B in a posture along the bottom surface.

Further, as shown in FIG. 2, the suction pressure by the suction tube a inserted into the test tube T is strengthened so that the reaction beads R and B in the test tube T are sucked up when the suction operation is performed. A receiver d for receiving the reaction beads R and B being sucked up is provided around the tip of the tube a, and the reaction tube R and B is strongly sucked in the test tube T by the suction tube a.
The reaction tubes R and B from the bottom surface of the reaction tube T
There is a means for sucking the cleaning liquid W by pulling it away from the bottom.

[Problems to be Solved by the Invention] In the above-mentioned conventional means, in the former means shown in FIG. 1, the flexible tube c on the lower end side of the suction tube a is a reaction bead R in the test tube T. Although it comes to enter the lower surface side of B and suck and remove all the cleaning liquid, the flexible tube c scrapes the surface of the reaction beads RB, The coated antibody surface will be damaged, making the measured value inaccurate and the flexible tube c during use.
Due to the irregular shape attached to the surface, there is a problem that the residual amount of the washing liquid is rather increased or the surface of the reaction beads RB is stuck, which seriously damages the antibody surface of the surface.

In the latter means shown in FIG. 2, the reaction beads R and B collide with the receiving d, and the antibody surface coated on the surface of the reaction beads R and B is damaged. There is a problem that comes to be.

[Purpose] The present invention has been made in order to solve the above-mentioned problems that have occurred in the conventional means, and does not damage the antibody surface coated on the surface of the reaction beads RB,
An object of the present invention is to provide a new means that allows the cleaning liquid W to be cleanly and efficiently sucked and removed from the bottom of the test tube T and the peripheral surfaces of the reaction beads R and B.

[Means for Solving the Problem] However, the present invention has been completed based on the findings obtained through various experiments and studies for solving the above-mentioned problems.

That is, when the cleaning liquid W is removed by suction from the inside of the test tube T,
As shown in FIGS. 3 and 4, at the center of the inside of the test tube T, the opening g of the blowout tube e leading to the compressed air source is arranged, and the opening g of the blowout tube e is An inverted funnel-shaped guide wall f, which gradually expands in diameter downwardly, is arranged around it, and when air is blown out from the opening g of the blow-out pipe e at a high pressure, it comes out from the opening g of the blow-out pipe e. The blown out compressed air blows out while adsorbing to the inverted funnel-shaped guide wall f, so that it blows out like an umbrella, and at that time, the negative pressure generated on the center side of the funnel-shaped guide wall f. Due to
As shown by the arrows in FIG. 3 and FIG. 4, the tip side of the compressed air that spreads and blows out like an umbrella bends back toward the center of the guide wall f, and blows up at the center. That the flow y of the compressed air is generated, and this flow y of the compressed air causes the reaction beads RB to float as shown in FIGS. 5 and 6 and rises. The reaction beads R and B do not rise from the position where a certain distance D is provided between the reaction beads R and B and the guide wall f due to the pressure of the compressed air ejected from the opening g of the blow-out pipe e. As it keeps floating in the air, the antibody surface on the surface is protected. The flow y of the above-mentioned compressed air also blows up by blowing up the bottom surface of the reaction beads RB floating in the air and the bottom of the inner surface of the tube wall of the test tube T.
The cleaning liquid W adhering to the lower peripheral surface of B and the inner surface of the pipe wall is blown up as a droplet, and as shown in FIG. 3, the suction port a of the suction pipe a disposed at the peripheral portion on the upper surface side of the guide wall f. 'Or, as shown in FIG. 4, the reaction beads R are carried to the suction port a'of the suction tube a which is arranged at the central portion on the lower surface side of the guide wall f.
The result is that the cleaning liquid can be sucked and removed cleanly from the peripheral surface of B and the inner wall surface of the test tube T.

However, in this method, the reaction beads RB floating in the air
May be balanced and may be in a stationary state. In such a case, the transport of the droplets blown up from the bottom of the test tube T to the suction port a'is suspended in the air and the reaction beads R. There was a problem that B took a long time to remove the cleaning liquid W cleanly.

Then, after further experimentation and research, when the opening g of the blow-out pipe E is biased toward one side and opened as shown in FIG. 7, the reaction beads R floating in the air・ B
Is slightly shifted in the direction in which the opening g is biased, and is rotated counterclockwise in FIG. 7 at a considerable speed to shake out the cleaning liquid adhering to the peripheral surface. In addition, the compressed air that flows along the inner surface of the tube wall of the test tube T below the compressed air flow y that floats the reaction beads R and B is indicated by the double-lined arrow z in FIG. As shown in the drawing, the washing liquid W remaining on the bottom of the inner surface of the tube wall of the test tube T becomes a flow Z that flows in one direction from the biased side of the opening g to the opposite side. As a result, it can be efficiently carried upward along the inner surface of the wall, and further, the blow-out tube e and the guide wall f are brought close to the bottom of the test tube T,
When the lower surface side of the reaction beads RB floating in the air is brought into contact with the liquid surface of the washing liquid W remaining at the bottom of the test tube T as shown in FIG. As a result, the cleaning liquid W adheres to the peripheral surface and is carried upward, which results in remarkably efficient removal of the remaining cleaning liquid by suction. The present invention has been completed based on these results.

From this, in the present invention, an inverted funnel-shaped guide wall f having a diameter gradually increasing downward is arranged in the test tube T in which the reaction beads R and B are dropped,
The opening g of the blow-out pipe e to be installed in the central portion on the lower surface side thereof
From the above, by blowing the compressed air toward the one side, the compressed air flow y is generated below the guide wall f so as to be blown up toward the center of the inside of the guide wall f. Then, the reaction beads R floating in the air by the flow y of the compressed air
Rotating B, the residual liquid of the cleaning liquid W that blows up from the bottom of the test tube T is swung upward by the rotation of the reaction beads R and B, and the blowout tube is formed at the central portion on the lower surface side of the guide wall f. A method for removing the washing liquid of reaction beads in the test of blood or the like, which is characterized in that suction is carried out at a suction port a ′ of a suction tube a provided at a peripheral portion on the upper surface side of the guide wall f or at a portion near the opening g of e. In addition, as an apparatus for carrying out this cleaning liquid removal method, a mounting table 1 for mounting a test tube T into which reaction beads R and B are dropped, and a blow-out tube for ejecting a pressurized air are provided. e and a dispensing head 2 equipped with a suction tube a and arranged above the placing table 1 are mounted on the machine body so that they are relatively separated from and contacting each other, and an outlet tube equipped to the dispensing head 2. Around the opening g of e, the diameter gradually increases downward In addition to disposing the funnel-shaped guide wall f, the opening g of the blow-out pipe e opening at the central portion of the guide wall f is arranged so as to be biased to one side, and the suction port a of the suction pipe a is arranged. ??? is a reaction test tube in an inspection of blood or the like, which is disposed in the central portion on the lower surface side of the guide wall f in the vicinity of the opening g of the blowout tube e or in the peripheral portion on the upper surface side of the guide wall f. Alternatively, it proposes an apparatus for removing the washing liquid of the reaction beads.

[Embodiment] Next, an embodiment will be described in detail with reference to the drawings. The same reference numerals as those of the conventional means are used for the constituent members having the same effect.

FIG. 9 is a longitudinal sectional view of a reaction test tube for testing blood and the like used in the present invention and a main part of a device A for washing reaction beads, in which T ... Is a test tube (or a reaction test tube). , R · B ... Reacted beads dropped into the test tubes T, 1 is a mounting table, 2 is a dispensing head, e is a blowing tube, f is a guide wall, a is a suction tube, 3 is a washing tube. Liquid tank, 4
Is a drainage tank, 5 is a vacuum pump, and 6 is a discharge pump.

The mounting table 1 is mounted on a body (not shown in the drawing) so that it can be raised and lowered by an elevating device 10 operated by a motor M.

The dispensing head 2 is arranged above the mounting table 1 and fixedly supported on the machine body. The dispensing head 2 may be mounted on a machine body so that it can be raised and lowered by an elevating device. In that case, the mounting table 1 described above may be fixed to the machine body.

The blow-out tube e ... rises when the test tube T (which may be a reaction test tube in which an antibody is coated on the inner surface of the tube) is positioned and mounted on the mounting table 1 by the lifting operation of the mounting table 1. Are arranged on the lower surface side of the dispensing head 2 such that they are inserted into the aligned test tubes T ... To the distribution pipes 20 to be installed in the
Is communicated with a discharge port of a vacuum pump 5 mounted on the machine body through a pipe line 21 having a valve V1 interposed therebetween. These blow pipes e
An inverted funnel-shaped guide wall f that gradually expands in diameter downwards is provided around the opening g at the lower end of the outlet g, and the opening g is located at the lower end of the blow-out pipe e at the central portion on the lower surface side thereof. It is located at. And the opening g of these blow-out pipes e is
It is arranged at a position slightly eccentric with respect to the center of the inverted funnel-shaped guide wall f that surrounds it, and is opened toward the eccentric direction. The bias of the opening g of the blow-out pipe e is not limited to the case where it is biased to the opening direction of the opening g as shown in FIG. 10, but the opening g is opened downward as shown in FIG. May be deviated from the center of the guide wall f, and as shown in FIG. 12, the tip end portion of the blow-out pipe e is penetrated from the upper surface side of the guide wall f toward the lower surface side, There is a case where the opening g is opened at a position slightly eccentric from the center on the lower surface side of the guide wall f so as to open in a direction parallel to the inner surface of the guide wall f on the opposite side.

The suction pipe a is formed in an outer cylindrical shape surrounding the blow-out pipe e, is arranged around each blow-out pipe e, and is provided on the lower surface side of the above-mentioned dispensing head 2. The center hole of the inverted funnel-shaped guide wall f is integrally connected to the lower end thereof. The inner surface of the guide wall f may be formed into an arc surface similar to the arc of the surface of the reaction beads R and B as shown in FIG. 13, in addition to the case where it is a flat taper surface. Then, each upper end side of the blow-out pipes e ... Is connected to and communicates with a collecting pipe 30 arranged in the dispensing head 2, and the collecting pipe 30 is provided with a communicating pipe 31 having a valve V2 interposed therebetween.
The suction side of the vacuum pump 5 is connected to and communicates with the drainage tank 4 that is in communication therewith. Further, the communication pipe 31 is formed by forming the valve V2 into a three-way valve, and is connected to the discharge side of the discharge pump 6 whose suction side communicates with the cleaning liquid tank 3 through the valve V2. The suction pipe a is connected to the discharge pump 6 which discharges the washing liquid by the switching operation of V2, and the suction pipe a is switched to the dispensing pipe b for use.

Next, FIG. 14 shows another embodiment of the apparatus. In this embodiment, the cleaning liquid W is supplied from the cleaning liquid tank 3 to each test tube T ...
This is an example in which a dispensing tube b for dispensing into a tube is provided separately from the suction tube a, and each dispensing tube b is arranged in parallel with each outlet tube e inside the outer tubular suction tube a. Each upper end side is provided in communication with a distribution pipe 70 installed in the dispensing head 2, and the distribution pipe 70 communicates with a discharge port of the discharge pump 6 via a communication pipe 71 having a valve V3. However, except that the collecting pipe 30 communicating with each suction pipe a communicates only with the drainage tank 4 via the communicating pipe 31 having the valve V2, the same as the above-described embodiment, the same components are the same. The reference numerals are given and detailed description is omitted.

Next, FIG. 15 is an enlarged vertical cross-sectional view of the suction pipe a, the dispensing pipe b, and the outlet pipe e used in the apparatus of the embodiment shown in FIG. A fitting portion 80 for mounting on the lower surface of the dispensing head 2 is formed, and a diameter-expanding cylinder portion 81 is connected to the lower end thereof, and the funnel shape is inverted at the lower end of the diameter-expanding cylinder portion 81. The guide wall f of is continuous. The open end at the lower end of the dispensing pipe b and the open end g at the lower end of the blow-out pipe e, which are stored inside the suction pipe a, are located inside the expanded diameter tube portion 81. The outlet pipe e in this embodiment has an outer diameter of 0.88 mm and an inner diameter of 0.58 mm, and the dispensing pipe b has an outer diameter of 1.06 mm and an inner diameter of 0.70 mm. Further, the suction pipe a has an inner diameter D1 of 2.4 mm on the upper end side and an inner diameter D2 of the expanded cylindrical portion 81 of 5.0 mm. Also,
The guide wall f has an inner diameter D3 of 7.0 mm at the lower end and a height H1.
Is 2.4 mm and the inclination angle α is 60 degrees. Also, test tube T
Has a height H2 of 20 mm and an inner diameter D4 of 8.8 mm.

[Effects of the Invention] As described above, the means of the present invention comprises reaction beads RB
An inverted funnel-shaped guide wall f whose diameter gradually increases downward is arranged in the test tube T in which the blow-in tube e is opened and the opening g of the blow-out tube e is installed in the central portion on the lower surface side of the guide wall f. From
By biasing the compressed air toward one side and ejecting the compressed air, the flow y of the compressed air generated so as to blow up toward the center inside the guide wall f is biased toward one side. , The reaction beads R and B floating in the air by the flow y of the pressurized air are rotated, and the residual liquid of the cleaning liquid W blown up from the bottom of the test tube T is spun out by the rotation of the reaction beads R and B. ,
By sucking at a central portion on the lower surface side of the guide wall f, a portion in the vicinity of the opening g of the blow-out tube e or a suction opening a ′ of a suction pipe a arranged at a peripheral portion on the upper surface side of the guide wall f, Since the residual liquid of the cleaning liquid W is removed by suction, the reaction beads R
-The residual liquid of the washing liquid W can be removed cleanly without contacting the antibody surface of the surface of B with the equipment, and the reaction beads R
Since B rotates to pump up the residual liquid at the bottom of the test tube T, the residual liquid of the cleaning liquid W can be efficiently sucked and removed in a short time.

Further, the cleaning liquid removing device according to the present invention is provided with the reaction beads R.
The mounting table 1 on which the test tube T on which B is dropped is mounted, and the dispensing head 2 which is equipped with a blow-out tube e and a suction tube a for ejecting compressed air and is arranged above the mounting table 1 described above, The dispensing head 2 is mounted on the machine body so that they are relatively separated from each other.
A guide wall f having an inverted funnel shape whose diameter gradually expands downward is disposed around the opening g of the blowout tube e equipped with the above-mentioned blowout tube e which is opened at the central portion of the guide wall f. Of the suction pipe a at a central portion on the lower surface side of the guide wall f, or a portion in the vicinity of the opening g of the blow-out pipe e. Guide wall f
Since the reaction beads R and B dropped into the test tube T are washed with the washing liquid W, the reaction beads R are sucked and removed because the reaction beads R and B are dropped into the test tube T.・
The reaction beads RB can be rotated remarkably efficiently without damaging the antibody surface on the surface of B.

[Brief description of drawings]

1 and 2 are explanatory views of the conventional means, FIGS. 3 and 4 are explanatory views of the prior art, and FIGS. 5 and 6 are explanatory views of the action on the reaction beads of the same prior art. 7 and 8 are explanatory views of the means of the present invention, FIG. 9 is a longitudinal sectional view of a main part of the cleaning liquid removing device used for carrying out the method of the present invention, and FIGS. 10 and 11 are parts of the same device. FIG. 12 is an enlarged vertical sectional view of another embodiment of the above-mentioned apparatus portion, FIG. 13 is an enlarged vertical sectional view of another embodiment of the above-mentioned apparatus portion, and FIG. FIG. 15 is a vertical cross-sectional view of the main part of the device of one embodiment, and FIG. 15 is an enlarged vertical cross-sectional view of the main part of the same device. Explanation of drawing symbols A: Washing device, D: Distance M: Motor, T: Test tube W: Washing liquid, V1-V3: Valve RB: Reaction beads, a: Suction tube a '... Suction port, b ... Dispensing pipe c ... Flexible pipe, d ... Reception e ... Blowing pipe, f ... Guide wall g ... Opening port, y ... Flow of compressed air 1 ... Mounting table, 10 ... Elevating device, 2 ... Dispensing head, 20 ... Dispensing pipe, 21 ... Pipe line, 3 ... Washing liquid tank, 30 ... Collecting pipe, 31 ... Communication pipe, 4 ... Drainage tank, 5 ... Vacuum pump 6 ... Discharge pump, 70 ... Distribution pipe 71 ... Communication pipe, 80 ... Mating part 81 ... Expanding cylinder part, H1, H2 ... Height D1-D4 ... Inner diameter

Claims (2)

[Claims] 1. An inverted funnel-shaped guide wall f whose diameter gradually increases downward is arranged in a test tube T into which reaction beads R and B are dropped, and a central portion on the lower surface side of the guide wall f is arranged. From the opening g of the blow-out pipe e to be installed, the compressed air is biased to one side and ejected, so that the compressed air is blown downward toward the center part inside the guide wall f. The flow y of the compressed air is biased to one side, the reaction beads R and B floating in the air are rotated by the flow y of the compressed air, and the residual liquid of the cleaning liquid W blown from the bottom of the test tube T is The reaction beads R and B
Of the suction pipe a, which is swayed upward by the rotation of the guide wall f and is disposed at a central portion on the lower surface side of the guide wall f in the vicinity of the opening g of the blow-out tube e or at a peripheral portion on the upper surface side of the guide wall f. A method for removing the washing liquid of the reaction beads in the inspection of blood and the like, which is characterized in that it is sucked into ′. 2. A test tube T into which reaction beads R and B are dropped.
The mounting table 1 on which the table is mounted and the dispensing head 2 which is equipped with the blow-out tube e and the suction tube a for ejecting the compressed air and is arranged above the mounting table 1 are relatively separated from each other. An inverted funnel-shaped guide wall f having a diameter gradually increasing downward is provided around the opening g of the blow-out pipe e mounted on the machine body and mounted on the dispensing head 2, and the guide wall is also provided. f
The opening g of the blow-out pipe e, which is open at the center of the blow-out pipe e, is arranged so as to be biased to one side, and the suction port a ′ of the suction pipe a is located at the center of the lower surface of the guide wall f. A cleaning test tube or reaction bead cleaning device for the inspection of blood or the like, which is disposed in a peripheral portion on the upper side of the guide wall f or a portion near the opening g of e.