JPH0635978B2 - Method and device for removing cleaning solution of reaction beads in inspection of blood, etc. - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention allows the coated antibody to react with a sample and a reagent in the test of blood and the like using reaction beads coated with antibody on the surface (reaction beads). When the reaction beads after washing are washed with a washing solution,
The present invention relates to an improvement in a cleaning liquid removal method and a cleaning liquid removal device for removing residual cleaning liquid.

[Prior Art] The washing of the reaction test tube or the reaction beads after the reaction, which is performed in the test of blood or the like using the reaction test tube having the inner wall surface coated with the antibody or the reaction bead having the surface coated with the antibody, is performed by the reaction test tube or It is for washing the sample or reagent that has come into contact with the reaction beads to measure the change status of the antibody.To make the measured value accurate, completely remove the washing solution used for washing. It is desirable to put it.

This cleaning solution can be removed by drying it with the cleaning solution adhering to the wall of the reaction test tube or the surface of the reaction beads, and then measuring the reaction test tube or reaction beads with an instrument such as a colorimeter. Since an error occurs when measuring the change, the drying means cannot be used and is removed by suction.

In the prior art, when the reaction test tubes R and T are washed, the suction / removal means for the cleaning liquid is a dispensing head that moves up and down relative to the reaction test tubes R and T as shown in FIG. The suction tube a provided in the reaction test tube R / T is arranged so as to be located on one side of the reaction test tube R / T, and the suction tube a is installed in the reaction test tube R / T more than the dispensing tube b which dispenses the washing liquid. It is provided so as to hang down for a long time so that the cleaning liquid remaining on the bottom of the pipe wall is forcibly sucked, or as shown in FIG.
When the flexible tube c which bends freely is connected to the lower end side of the suction tube a and the suction tube a is inserted into the reaction test tube R / T, the flexible tube c on the lower end side undergoes the reaction test. The pipes R and T are bent along the inner surface of the pipe wall so as to be in a posture along the arcuate bottom face, and the cleaning liquid remaining at the bottom is sucked and removed completely.

In washing the reaction beads R and B, normally, as shown in FIG. 3, the suction tube a that moves up and down with respect to the test tube T is moved from the dispensing tube b in the same manner as the means of FIG. Longer and
The first means for arranging along the inner surface of the test tube T and the flexible tube c on the lower end side of the suction tube a as shown in FIG.
A second means is used which uses the means of FIG.

Of these means, the first means is that the cleaning liquid W is inevitably left behind on the lower surface side of the reaction beads R and B in the test tube T, and the second means is that the flexible tube c is used for the reaction beads R.・
The problem that the surface of B becomes sticky, the antibody layer on the surface is damaged, and suction and removal of the cleaning liquid W is incomplete due to the irregular shape of the flexible tube c during use. There is.

From this, as shown in FIG. 5, the suction pressure by the suction tube a inserted into the test tube T is increased so that the reaction beads R and B in the test tube T float during the suction operation. At the same time, a receiver d for holding the floating reaction beads R and B is provided around the tip of the suction tube a, and the reaction beads R and B that float are supported on the receiver d.
A means for sucking and removing the cleaning liquid W on the lower surface side of B has been developed, and as shown in FIG. 6, the suction tube a is arranged so as to be inserted into the central portion inside the test tube T. At the same time, the bottom end of the suction tube a is provided with an inverted funnel-shaped receiver d, and the suction beads a strongly suck the inside of the test tube T so that the reaction beads R and B are floated. A means for receiving the cleaning liquid W on the lower surface side of the reaction beads R and B and sucking it so as to dredge the cleaning liquid W has been tried.

[Problems to be Solved by the Invention] The means shown in FIG. 5 and FIG.
Since B is floated from the bottom of the test tube T, the washing liquid W on the lower surface side of the reaction beads R and B can be sucked as if it is dredging, but the reaction beads R and B to be floated
Since it is supported by d at a constant height position due to the rise of the above, there is a problem that the antibody layer coated on the surface of the reaction beads RB is damaged.

[Purpose] The present invention has been made in order to solve the above-mentioned problems that have occurred in the conventional means, and does not damage the antibody layer on the surface of the reaction beads R and B, and the bottom portion of the test tube T and It is an object of the present invention to provide a new means for completely removing the cleaning liquid W from the peripheral surface of the reaction beads R and B by suction.

[Means for Solving the Problem] However, the present invention has been completed based on the findings obtained through various experiments and studies for solving the above-mentioned problems.

That is, when the cleaning liquid W is sucked and removed from the reaction test tubes R and T, as shown in FIG. 7, the blowout tube e communicating with the compressed air source is formed at the central portion of the reaction test tubes R and T. And the central portion of the funnel-shaped guide wall f that is inverted to the edge of the opening g at the lower end of the blow-out pipe e is continuous,
When air is blown out from the blower tube at a high pressure, the compressed air blown out from the blowout tube e is blown out while being adsorbed by the inverted funnel-shaped guide wall f, as shown by the arrow a in FIG. As a result of the negative pressure generated on the central part side of the funnel-shaped guide wall f at the time of being expanded and blown out in an umbrella shape, as shown by the arrow in FIG. The side bends back toward the center of the guide wall f, and a flow y of compressed air that rises up at the center is generated, and this flow y of compressed air is the reaction test tube R · T.
The residual liquid of the cleaning liquid W adhering to the bottom portion of the tube wall of No. 2 is collected in the central portion of the bottom of the reaction test tube R / T so that it can be blown up as a droplet, , As shown in FIG. 7 above the guide wall f of the blow-out pipe e, or as shown in FIG. 8, sucked by the suction port of the suction pipe a which is arranged at the central portion of the opening g of the blow-out pipe e. Iki, reaction test tube R
・ Washing liquid W that remains on the bottom of the inner surface of the T pipe wall
As a result, it was possible to cleanly remove by suction without rubbing the inner surface of the tube wall with the device.

Then, the funnel-shaped guide wall f which is thus inverted to the opening
Was used for suction removal of the washing liquid W that had washed the reaction beads R and B dropped in the test tube T. As shown in FIG. A flow y of compressed air, which is blown out from the opening and is expanded in an umbrella shape by the funnel-shaped guide wall f that is inverted and is generated toward the center by the compressed air that blows out, causes the reaction beads R and B to float. And, the floating reaction beads R and B are blown out from the outlet pipe e.
The pressure of the compressed air blown out from the opening g does not cause it to rise above the position where a certain distance D is formed between it and the guide wall f, and to maintain the state of floating in the air as shown in FIG. The result was obtained. That is, the result was that the reaction beads R and B were raised without contacting the other layer with the antibody layer coated on the surface of the reaction beads R and B.

Then, in the test tube T, the above-mentioned compressed air flow y
However, as shown in FIG. 10, the reaction beads R and B are blown up by drenching up the lower surface of the reaction beads R and B and the bottom of the inner surface of the tube wall of the test tube T. The cleaning liquid W adhering to the lower peripheral surface and the inner surface of the pipe wall is to be wiped off, and the cleaning liquid W to be wiped off is sprayed and blows up, whereby the cleaning liquid W is wiped out. The upper surface side of the guide wall f of the blow pipe e or the guide wall f
As a result, the cleaning liquid can be sucked and removed cleanly from the peripheral surface of the reaction beads R and B and the inner wall surface of the test tube T by being sucked into the suction port of the suction tube a provided in the central portion inside the container. Is obtained.

From this, in the present invention, an inverted funnel-shaped guide wall f having a diameter gradually increasing downward is arranged in the test tube T in which the reaction beads R and B are dropped,
The opening g of the blow-out pipe e to be installed in the central portion on the lower surface side thereof
By blowing out a pressured air from the cleaning liquid W in the test tube T toward the center of the space on the lower surface side of the guide wall f,
It is characterized in that suction is carried out at a central portion on the lower surface side of the guide wall f, to a suction port 33 of a suction tube a arranged at a portion in the vicinity of the opening g of the blowout pipe e or at a peripheral portion on the upper surface side of the guide wall f. The present invention proposes a method for removing the washing liquid of reaction beads in the inspection of blood and the like. At the same time, the reaction beads R are used as an apparatus for carrying out this cleaning liquid removal method.
A mounting table 1 on which the test tube T on which B has been dropped is mounted, and a dispensing head 2 which is equipped with a blowing tube e and a suction tube a for ejecting compressed air and is arranged above the mounting table 1. Are mounted on the machine body so that they are relatively separated from each other, and an inverted funnel-shaped guide that gradually expands downward around the opening g of the blow-out pipe e mounted on the dispensing head 2. A wall f is provided, and the suction pipe a has its suction port 33 at a central portion on the lower surface side of the guide wall f in the vicinity of the opening g of the blowout pipe e or on the upper surface side of the guide wall f. The present invention provides an apparatus for removing a cleaning solution of reaction beads in an inspection of blood or the like which is placed at a site.

[Embodiment] An embodiment will be described in detail with reference to the drawings. The same reference numerals as those of the conventional means are used for the constituent members having the same effect.

FIG. 11 is a longitudinal sectional view of a main part of a device A for washing reaction beads for testing blood or the like used for carrying out the present invention, in which T ... Is a test tube and R.B ... is a test tube T thereof. Reaction beads dropped into the inside of the chamber, 1 mounting table, 2 dispensing head, e blowing pipe, f guiding wall, a suction pipe, 3 washing tank, 4 drainage tank, 5 Is a vacuum pump, and 6 is a discharge pump.

The mounting table 1 is mounted on a body (not shown in the drawing) so that it can be raised and lowered by an elevating device 10 operated by a motor M.

The dispensing head 2 is arranged above the mounting table 1 and fixedly supported on the machine body. The dispensing head 2 may be mounted on a machine body so that it can be raised and lowered by an elevating device. In that case, the mounting table 1 described above may be fixed to the machine body.

The blow-out tubes e ... Are inserted into the aligned test tubes T ... When the test tubes T, which can be positioned and placed on the mounting table 1, are lifted by the lifting operation of the mounting table 1. Are arranged on the lower surface side of the dispensing head 2, and their upper ends communicate with the distribution pipes 20 installed in the dispensing head 2, respectively. It communicates with a discharge port of a vacuum pump 5 mounted on the machine body via a pipe line 21 provided with a valve V1. And, as shown in FIG. 7 described above, the opening g at the lower end of the blow-out pipe e is
Inverted funnel-shaped guide wall f that gradually expands in diameter downward
The central part of is connected and communicates.

The suction pipe a is formed in an outer cylinder shape surrounding the blow-out pipe e, is arranged around each blow-out pipe e, and is mounted on the lower surface side of the above-mentioned dispensing head 2. The upper end side of each of them is connected and communicated with a collecting pipe 30 arranged in the dispensing head 2. Then, the collecting pipe 30 is connected and communicated with the drainage tank 4 to which the suction side of the vacuum pump 5 is communicated, by a communication pipe 31 having a valve V2. Further, the communication pipe 31 has the above-mentioned valve V2 formed as a three-way valve,
The valve V2 is connected to the discharge side of the discharge pump 6 whose suction side communicates with the cleaning liquid tank 3, and communicates with the discharge pump 6 which discharges the cleaning liquid by the switching operation of the valve V2. The cleaning liquid is supplied to a and the suction pipe a is switched to the dispensing pipe b for use. The suction pipe a is connected at its lower end with an umbrella-shaped guide wall 32 formed in a shape similar to the guide wall f and arranged on the upper surface side of the guide wall f. The annular slit formed between the peripheral edge of the guide wall 32 and the peripheral edge of the guide wall f constitutes the suction port 33 of the suction pipe a. As shown in FIG. 12, the suction port 33 is formed by forming the umbrella-shaped guide wall 32 with substantially the same diameter as the guide wall f,
In the case where the guide wall 32 is opened toward four turns, as shown in FIG. 13, the umbrella-shaped guide wall 32 is formed to have a larger diameter than the guide wall f, and the guide wall 32 is directed toward the lower surface side of the guide wall 32. May be opened. In addition, the opening g of the outlet pipe e
The pressure of the compressed air ejected from the suction port 33 and the suction pressure of the suction port 33 of the suction pipe a are 10% to 10% of the pressure of the compressed air ejected from the opening g of the blow pipe e.
It is set to be about 20%.

The embodiment apparatus configured as described above operates as follows.

From the state in which the cleaning liquid W is dispensed into the test tubes T ... As shown in FIG. 14, the communication tube 31 communicating with the suction tubes a is connected to the suction side of the vacuum pump 5 by the switching operation of the valve V2. When the mounting table 1 is moved up while the valve V1 is kept closed while being connected to the drainage tank 4 communicating with the test tube T ... As it is inserted into the inside, the cleaning liquid W inside is sucked up and collected in the drainage tank 4. Then, when the guide wall f provided around the opening g of the blow-out tube e descends slightly above the reaction beads R and B in the test tube T as shown in FIG. 18 is stopped and the mounting table 1 is lowered a little, whereby the state shown in FIG. 18 is reached. In this state, the valve V1 is opened and the compressed air discharged from the discharge port of the vacuum pump 5 becomes thin. Squeezed outlet tube e
So that it spouts from the opening g.

As a result, the compressed air blown out spreads and blows out in an umbrella shape by the funnel-shaped guide wall f, and the compressed air blows up toward the center of the guide wall f as shown by the arrow in FIG. The flow y of the compressed air causes the reaction beads R and B to float and float in the space slightly below the guide wall f, and at the same time, the inner surface of the tube wall of the test tube T is generated. Of the cleaning liquid W adhering to the inner surface of the tube wall and the peripheral surfaces of the reaction beads R and B is blown up into the droplets of the suction port 33 of the suction tube a. Will be carried to.

As a result, the bottom of the test tube T and the reaction beads RB
When the remaining liquid on the peripheral surface of the
As the pressure is lowered, the suction pipe a and the discharge pipe e are pulled up above the test tube T, and the operation for sucking and removing the residual liquid is completed. At this time, the suction port 33 of the suction tube a rises while sucking and removing the residual liquid adhering to the upper part of the inner surface of the tube wall of the test tube T (Fig. 21).

Next, FIG. 23 shows another embodiment of the apparatus. In this embodiment, the cleaning liquid W is supplied from the cleaning liquid tank 3 to each test tube T ...
This is an example in which a dispensing tube b for dispensing into a tube is provided separately from the suction tube a, and each dispensing tube b is arranged in parallel with each outlet tube e inside the outer tubular suction tube a. Each upper end side is provided in communication with a distribution pipe 70 installed in the dispensing head 2, and the distribution pipe 70 communicates with a discharge port of the discharge pump 6 via a communication pipe 71 having a valve V3. The suction pipe a communicates with only the drainage tank 4 via the valve V2. The other points are the same as those in the above-described embodiment, and the same components are designated by the same reference numerals and detailed description thereof will be omitted.

[Effects of the Invention] As described above, the means of the present invention is the cleaning liquid W remaining between the bottom of the inner surface of the tube wall of the test tube T and the lower surface of the reaction beads R and B.
In order to remove the above, the compressed air is blown toward the bottom of the test tube T from the central portion of the inverted funnel-shaped guide wall f that gradually expands in the downward direction. A flow y of compressed air that is blown up toward the side is generated, and the reaction beads R and B are generated by the flow y and the compressed air that flows downward along the lower surface of the guide wall f from the central portion of the guide wall f. The surface coated with the antibody layer of 1 is raised so that it floats in the air away from other objects, and the residual liquid adhering to the bottom surface of the test tube T and the peripheral surfaces of the reaction beads RB is splashed. Since the suction port 33 of the blow-up suction pipe a is sucked in a uniform manner, the residual liquid can be suctioned and removed cleanly without damaging the antibody layer on the surface of the reaction beads RB. Become.

[Brief description of drawings]

FIG. 1 is an explanatory view of a conventional cleaning solution removing means for a reaction test tube,
FIG. 2 is an explanatory view of another means of the above, FIG. 3, FIG. 4, FIG. 5, and FIG. 6 are explanatory views of the conventional means for removing the washing liquid of the reaction beads, and FIG. 7 is according to the present invention. Explanatory drawing of the cleaning liquid removing means of the reaction test tube, FIG. 8 is an explanatory view of another example of the above
9 and 10 are explanatory views of the cleaning solution removing means for reaction beads according to the present invention, FIG. 11 is a vertical sectional side view of a main part of a cleaning device used for carrying out the present invention, and FIG. 12 is a part of the same device. FIG. 13 is an enlarged vertical cross-sectional view of a modification of the above-mentioned device portion, FIG. 14, FIG. 15, FIG. 16, FIG.
7, FIG. 18, FIG. 19, FIG. 20, FIG. 21, and FIG.
FIG. 2 is an explanatory view of the operation of the same apparatus, and FIG. 23 is a longitudinal sectional view of a main part of another embodiment of the same apparatus. Explanation of reference numerals A ... Washing device, D ... Distance M ... Motor, T ... Test tube W ... Washing liquid, V1 to V3 ... Valve R / T ... Reaction test tube, R / B ... Reaction beads a ... Suction tube, b ... Dispensing pipe c ... Flexible pipe, d ... Receiving e ... Blowing pipe, f ... Guide wall g ... Opening port, y ... Flow of compressed air 1 ... Mounting table, 10 ... Lifting device 2 ... Dispensing head, 20 ... Distributing pipe 21 ... Pipe line, 3 ... Washing liquid tank 30 ... Collecting pipe, 31 ... Communication pipe 32 ... Guide wall, 33 ... Suction port 4 ... Drainage tank, 5 ... Vacuum pump 6 ... Discharge pump, 70 ... Distributing pipe 71 … Communication pipe

Claims (2)

[Claims] 1. An inverted funnel-shaped guide wall f whose diameter gradually increases downward is arranged in a test tube T into which reaction beads R and B are dropped, and a central portion on the lower surface side of the guide wall f is arranged. A pressurized air is blown from the opening g of the blow-out pipe e to be installed, and the residual liquid of the cleaning liquid W in the test tube T is blown up toward the center of the space on the lower surface side of the guide wall f, and the guide wall f The suction port 3 of the suction pipe a, which is disposed in the central region on the lower surface side of the suction pipe e in the vicinity of the opening g of the blow-out pipe e or on the peripheral portion on the upper surface side of the guide wall f.
3. A method for removing a cleaning liquid of reaction beads in an inspection of blood or the like, which is characterized in that the liquid is sucked by 3. 2. A test tube T in which reaction beads R and B are dropped.
The mounting table 1 on which the table is mounted and the dispensing head 2 which is equipped with the blow-out tube e and the suction tube a for ejecting the compressed air and is arranged above the mounting table 1 are relatively separated from each other. An inverted funnel-shaped guide wall f, which gradually expands downward, is arranged around the opening g of the blow-out pipe e mounted on the machine body and mounted on the dispensing head 2. a is a blood or the like formed by arranging its suction port 33 at the central portion on the lower surface side of the guide wall f in the vicinity of the opening g of the blowout tube e or on the peripheral portion on the upper surface side of the guide wall f. Device for removing washing liquid of reaction beads in inspection.