JPH0720844B2 - How to sterilize and remove germs - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for sterilizing and removing bacteria.

(Prior Art) In our living space, various microorganisms such as bacteria, molds and bacteria are present. And in a hot and humid environment, their reproduction is particularly active, causing the phenomena such as fiber deterioration, discoloration, and deterioration, causing spoilage and fermentation, and generating an unpleasant odor. There is.

Also, despite the fact that modern surgery uses specialized technology and extremely complicated equipment, wound infections continue to be numerous, and it is one of the items of great interest in hospitals.
Therefore, it prevents post-operative infection by pathogenic bacteria and is useful for wound healing, and when administering drugs etc. to the body, it is a material to prevent pathogenic microbial invasion from the route and medical treatment to make patients' illness life easier. The development of textile products for use was desired.

Conventionally, as an antibacterial and antifungal processing method, a method of applying or spraying a compound having an antibacterial activity to natural or synthetic fibers, for example, a quaternary ammonium salt, or impregnating the fibers with a compound solution is known. However, in these methods, the effect is not sustainable, and the antibacterial agent is easily removed by subsequent washing or rubbing, which is a problem in terms of safety and hygiene and wastewater pollution. In addition, when the resin processing is performed using a resin to which an antibacterial agent is added, there is a drawback that the feel of the fiber is impaired.

(Problems to be Solved by the Invention) The present invention provides a method for selectively sterilizing and removing bacteria contained in air, water, an aqueous solution, and blood.

(Means for Solving Problems) The present invention has the following configurations.

A method for sterilizing and removing a bacterium, which comprises contacting a bacterium or a medium containing the bacterium with a polymer having a functional group of the following general formula (1) as a substituent in a main chain or a measuring chain or a molded product thereof. .

In the above formula, R ₁ and R ₂ represent a hydrogen atom or a lower alkyl group, and R ₃ represents an alkylene group having a methylene chain length of 6 or more. R ₄ and R ₅ represent a hydrogen atom or a lower alkyl group.

Examples of the bacterium in the present invention include gram-negative bacteria such as gram-negative cocci, gram-negative aerobic bacilli, and gram-negative aerobic bacilli, and gram-positive bacteria represented by Staphylococcus aureus.

The polymer in the present invention means a polymer such as aromatic polyamide, polyester, polysulfone, polyphenylene sulfide, polystyrene, etc. Among them, polystyrene is particularly preferable because it is chemically stable. Further, if the polymer is reinforced with poly α-olefin represented by crystalline polypropylene, polyethylene, etc., it is more preferable because the mechanical properties are improved.

In the general formula (1), R ₁ and R ₂ include a hydrogen atom or a lower alkyl group such as a methyl group and an ethyl group.
Of these, hydrogen atoms are the easiest to manufacture. See also R ₃
It is better to have 6 or more methylene chains because the ability to treat bacteria will be greater, but if the methylene chain length is 24 or more, the raw material is difficult to obtain. Further, when R ₄ and R ₅ are a hydrogen atom or a methyl group, it is easy to produce.

The amount of the functional group represented by the general formula (1) in the polymer is not particularly limited, but if it is too small, the compatibility of the polymer or its molded product with the bacterium or the medium containing the bacterium may be improved. Since it deteriorates and the processing ability decreases, it is preferable that the amount is 0.2 mmol or more, and more preferably 0.6 mmol or more per 1 g of the polymer.

In addition, the polymer or its molded product is substantially inactive under the conditions of use and is substantially inactive. That is, unlike the case of imparting the sterilizing power by the chemical treatment or the surface treatment, it is safe because it itself retains the sterilizing power. For this reason, the effect continues even after long-term use, and its industrial utility value is extremely high.

The term "molded product" as used in the present invention means a fiber, a membrane, a hollow fiber, a granular material, and a highly processed product thereof. If it is a fiber, it can be used in a higher-order form such as woven fabric, knitted fabric, paper, felt, and filter. In particular, it is preferable that fibers and hollow fibers can be used in such a manner that a flow path can be secured.

This property is important when the molded product is used as a treating agent for a highly viscous liquid such as blood. In this case, if the surface area of the molded product is too small, the treatment capacity for bacteria will be reduced, and if it is too large, the liquid permeability of the column packed with the molded product of the present invention will be poor. Surface area is 0.01 or more 50
m ² / g or less, more preferably 0.05 or more and 10 m ² / g or less.

Specific examples of the method for preparing a molded article according to the present invention include polystyrene fiber reinforced with polypropylene having a multi-core sea-island structure and paraformaldehyde and N-methylol-α-chloroacetamide at room temperature in the presence of sulfuric acid and nitrobenzene. Amine represented by the following general formula (2) dissolved in an aprotic polar solvent represented by dimethyl sulfoxide, N, N-dimethylformamide, N, N-dimethylacetamide, etc. It can be achieved by immersing in the solution of.

Illustrating a method of sterilizing and removing bacteria from a medium containing a bacterium or a bacterium using the molded article according to the present invention, the molded article is contacted with the bacterium or a medium containing a bacterium, and then the molded article is separated. do it. As a method of contact, a column filled with the molded product is prepared, and a medium containing a bacterium or a bacterium is passed through the column, or the molded product is made into a felt-like or filter-paper-like product, and a medium containing the bacterium or a bacterium. The method of filtering out is also preferably used. The molded product may be used alone or in combination with a product such as a porous membrane.

Examples of the use of the molded article according to the present invention include infusion fluid, dialysate or blood, physiological saline in a column filled with the molded article,
There are a method of circulating an aqueous solution, water, air and the like, and a method of coating the surface of a burned part or a scratched part with the molded product. Further, as an example of a product obtained from the molded product, as a medical-related fiber product, a blood contact material, an apron, a bed cover, a diaper, a surgical cover, a face mask, a feminine hygiene device, an incontinence pad, There are laboratory clothes, bags for laundry items, bandages, sheets, pillow cases, bed covers, surgical clothes, and sutures. In addition to medical items, clothes, wallpapers,
Examples include carpets, food storage containers, food packaging tools, and vacuum cleaner garbage bags.

Examples will be shown below.

(Example) (Preparation of material for sterilization and removal of bacteria) 50 parts of polypropylene (Mitsui "Nobren" J3HG) as an island component, 46 parts of polystyrene ("Stylon" 666), polypropylene (Sumitomo "Nobren" WF-727-F) ) Sea-island type composite fiber containing 4 parts of mixture as sea component (16 islands, single yarn fineness)
2.6 denier, tensile strength 2.9 g / d, elongation 50%, filament number 42) 50 g in a mixed solution consisting of 50 g of N-methylol-α-chloroacetamide, 400 g of nitrobenzene, 400 g of 98% sulfuric acid and 0.85 g of paraformaldehyde. Soak, 2
The reaction was carried out at 0 ° C for 1 hour. Remove the fiber from the reaction solution,
The reaction mixture was poured into 5 liters of ice water at 0 ° C. to stop the reaction, then washed with water, and then nitrobenzene attached to the fiber was extracted and removed with methanol. This fiber was vacuum dried at 50 ° C. to obtain 71 g of chloracetamidomethylated fiber (fiber A).

Hexamethylenediamine (94 g) was dissolved in 376 g of dimethylsulfoxide, and the fiber A (16 g) obtained above was put into this solution and immersed in the solution at a temperature of 20 ° C. for 48 hours. N- (ω-aminohexyl) glycylaminomethylated yarn / hydrochloride was obtained. The amount of weakly basic groups of this yarn is 2.83meq / g, water content 1.10 / PH7.4, 1.90 /
It was a hydrochloric acid type.

Further, N- (ω-aminoundecamethyl) glycylaminomethylated yarn which is the fiber of Example 2 of the present invention was treated in exactly the same manner as above except that undecamethylenediamine was used in place of hexamethylenediamine. -Hydrochloride was obtained.
The weakly basic group content of this thread is 1.61 meq / g, water content 0.59 / PH7.4,
It was 0.76 / hydrochloric acid type.

(Measurement method of viable cell count) Bacteria of Escherichia coli (ATCC25922) were suspended in a sterilized phosphate buffer to prepare a concentration of about 10 ⁶ CFU / ml (cold forming unit). After shaking the fibers and bacterial liquid, bacteria solution 3 serial dilutions (10 ^0, 10 ^2, 10 ⁴ dilution), and each 0.1 ml DHL agar medium (Nissui Pharmaceutical Co., Nissui Plate DHL agar)
Was inoculated. After culturing at 37 ° C for 24 hours, the number of colonies was measured.

Example 1 The following sterilization and bacterium removal experiments were carried out on the fibers of Examples 1 and 2 of the present invention and the comparative fibers obtained in the examples. Cut 0.25 g of the prepared fiber to a length of 3 cm, put it in a 10 ml glass test tube, add 8 ml of distilled water, plug it, and put it at 121 ℃.
Min autoclaved. After that, the supernatant was removed, 5 ml of the bacterial solution was added, and the mixture was shaken at room temperature and sampled at predetermined intervals. As a control, a test tube into which only the bacterial solution was injected was prepared and shaken in the same manner. After shaking for a predetermined time, the viable cell count was measured and the results shown in Table 1 were obtained.

From Table 1, it can be seen that the number of viable bacteria decreases or becomes zero in the examples of the present invention with the passage of time, whereas in the comparative example, the number of viable bacteria does not decrease and it is ineffective.

The fiber of the comparative example was prepared as follows. Fiber A5
0 g was immersed in 1.5 l of ethylenediamine at 20 ° C., and allowed to stand at room temperature for 4 days for reaction. After the reaction, the yarn was taken out and sufficiently washed with water to obtain a N- (ω-aminoethyl) glycylaminomethylated yarn as a comparative fiber. The weakly basic group content of this fiber was 2.92 meq / g, water content 1.12 / PH7.4, 1.51 / hydrochloric acid type).

(Effect of the present invention) The present invention can reduce the contamination level of bacteria, control wound infection, and make the wound sterile. Moreover, since it does not elute from the material, it is non-toxic, non-sensitizing and non-irritating, and its antibacterial activity does not decrease during use, so it is safe and easy to handle.

Claims (1)

[Claims] 1. A sterilizer, which comprises contacting a bacterium or a medium containing the bacterium with a polymer having a functional group of the following general formula (1) as a substituent in a main chain or a side chain or a molded product thereof. How to remove bacteria. In the above formula, R ₁ and R ₂ represent a hydrogen atom or a lower alkyl group, and R ₃ represents an alkylene group having a methylene chain length of 6 or more. R ₄ and R ₅ represent a hydrogen atom or a lower alkyl group.