JPH0720845B2 - How to sterilize and remove germs - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for sterilizing and removing bacteria.

(Prior Art) In our living space, various microorganisms such as bacteria, molds and bacteria are present. And in a hot and humid environment, their reproduction is particularly active, causing the phenomena such as fiber deterioration, discoloration, and deterioration, causing spoilage and fermentation, and generating an unpleasant odor. There is.

Also, despite the fact that modern surgical techniques and extremely complicated equipment are used for surgery, wound infections are still prevalent, and it is one of the items of great interest in hospitals.
Therefore, it prevents postoperative infections due to hospital bacteria and is useful for wound healing, and when administering drugs, etc. to the body, it is a material to prevent invasion of pathogenic microorganisms through that route and makes the patient's fighting life easier. Development of medical fiber products has been desired.

Conventionally, as an antibacterial and antifungal processing method, a method of applying or spraying a compound having an antibacterial activity to natural or synthetic fibers, for example, a quaternary ammonium salt, or impregnating the fibers with a compound solution is known. . However, in these methods, the effect is not sustainable, and the antibacterial agent is easily removed by subsequent washing or rubbing, which is a problem in terms of safety and hygiene and wastewater pollution. In addition, when the resin processing is performed using a resin to which an antibacterial agent is added, there is a drawback that the feel of the fiber is impaired.

(Problems to be Solved by the Invention) The present invention provides a method for selectively sterilizing and removing bacteria contained in air, water, an aqueous solution, and blood.

(Means for Solving Problems) The present invention has the following configurations.

A method for sterilizing and removing a bacterium, which comprises contacting a bacterium or a medium containing the bacterium with a polymer having a functional group of the following general formula (1) as a substituent in a main chain or a side chain or a molded article thereof. .

In the above formula, R ₁ and R ₂ represent a hydrogen atom or a lower alkyl group.

Hereinafter, the present invention will be described in detail.

Examples of the bacterium used in the present invention include gram-negative bacteria such as gram-negative cocci, gram-negative aerobic bacilli, and gram-negative anaerobic bacilli, and gram-positive bacteria and fungi represented by Staphylococcus aureus.

The polymer in the present invention means a polymer such as aromatic polyamide, polyester, polysulfone, polyphenylene sulfide, polystyrene, etc. Among them, polystyrene is particularly preferable because it is chemically stable. Further, if the polymer is reinforced with poly α-olefin represented by crystalline polypropylene, polyethylene, etc., it is more preferable because the mechanical properties are improved.

In the general formula (1), R ₁ and R ₂ include a hydrogen atom or a lower alkyl group such as a methyl group and an ethyl group.
Of these, hydrogen atoms are the easiest to manufacture.

The amount of the functional group represented by the general formula (1) in the polymer is not particularly limited, but if the amount is too small, the affinity of the polymer or its molded product with the bacterium or the medium containing the bacterium is poor. Therefore, the treatment capacity is lowered, so that it is preferable that the amount is 0.2 mmol or more, and more preferably 0.6 mmol or more per 1 g of the polymer.

In addition, the polymer or its molded product is substantially inactive under the conditions of use and is substantially inactive. That is, unlike the case of imparting the sterilizing power by the chemical treatment or the surface treatment, it is safe because it itself retains the sterilizing power. Therefore, the effect continues even if used for a long time, so that the industrial utility value is extremely high.

The term "molded product" as used in the present invention means a fiber, a membrane, a hollow fiber, a granular material, and a highly processed product thereof. Further, it can also be used in higher-order forms such as fibers and woven fabrics, knitted fabrics, papers, felts and filters. In particular, it is preferable that fibers and hollow fibers can be used in such a manner that a flow path can be secured. This property is important when the molded product is used as a treating agent for a highly viscous liquid such as blood. In this case, if the surface area of the molded product is too small, the treatment capacity of the bacteria will be reduced, and if it is too large, the air permeability of the column packed with the molded product of the present invention will be poor. Is 0.1 or more and 50 m ² / g or less,
More preferably, it is 0.05 or more and 10 m ² / g or less.

Specific examples of the method for preparing a molded article according to the present invention include polystyrene fiber reinforced with polypropylene having a multi-core sea-island structure in the presence of sulfuric acid and nitrobenzene at room temperature and paraformaldehyde and N-methylol-α-chloro. After insolubilization and amidomethylation using acetamide, it was immersed in hydrous ethanol containing potassium iodide at 55 ° C.
It can be achieved by heating for 4 hours.

Illustrating a method of sterilizing and removing bacteria from a medium containing a bacterium or a bacterium using the molded article according to the present invention, the molded article is contacted with the bacterium or a medium containing a bacterium, and then the molded article is separated. do it. As a method of contact, a column filled with the molded product is prepared, and a medium containing a bacterium or a bacterium is passed through the column, or the molded product is made into a felt-like or filter-paper-like product, and a medium containing the bacterium or a bacterium. The method of filtering off is also preferably used. The molded product may be used alone or in combination with a product such as a porous membrane.

Examples of the use of the molded article according to the present invention include infusion fluid, dialysate or blood, physiological saline in a column filled with the molded article,
There are a method of circulating an aqueous solution, water, air and the like, and a method of coating the surface of a burned part or a scratched part with the molded product. Further, as an example of a product obtained from the molded product, as a medical-related fiber product, a blood contact material, an apron, a bed cover, a diaper, a surgical cover, a face mask, a feminine hygiene device, an incontinence pad, There are laboratory clothes, bags for laundry, wrapping bandages, sheets, pillow cases, bed covers, surgical clothes, and sutures. In addition to medical use, clothing, wallpaper,
Examples include carpets, food storage containers, food packaging tools, and vacuum cleaner garbage bags.

Examples will be shown below.

(Example) (Preparation of material for sterilization and removal of bacteria) 50 parts of polypropylene (Mitsui "Nobren" J3HG) as an island component, 46 parts of polystyrene ("Stylon" 666), polypropylene (Sumitomo "Nobren" WF-727-F) ) Sea-island type composite fiber containing 4 parts of mixture as sea component (16 islands, single yarn fineness)
2.6 denier, tensile strength 2.9 g / d, elongation 50%, filament number 42) 50 g, mixed solution consisting of N-methylol-α-chloroacetamide 50 g, nitrobenzene 400 g, 98% sulfuric acid 400 g and paraformaldehyde 0.85 g. Invades inside, 2
The reaction was carried out at 0 ° C for 1 hour. Remove the fiber from the reaction solution,
The reaction mixture was poured into ice water 51 at 0 ° C. to stop the reaction and then washed with water, and then nitrobenzene attached to the fiber was extracted and removed with methanol. This fiber was vacuum dried at 50 ° C to give 71 g of chloracetamidomethylated fiber (raw fiber).
Got

40 g of this chloroacetamide methylated fiber was soaked in a mixed solution of 130 g of potassium iodide, 800 ml of ethanol and 240 ml of water, heated at 55 ° C. for 4 hours to iodize it, and then washed thoroughly with ethanol and water to prepare an inventive example. Iodoacetamide methylated fiber (water content 0.31 / P
H7.4, 0.32 / hydrochloric acid type) was obtained.

(Method for measuring viable cell count) Escherichia coli (Escherichia coli ATCC 25922) was suspended in a sterilized phosphate buffer to prepare a concentration of about 10 ⁶ CFU / ml (cold forming unit). After shaking the fibers and bacterial liquid, bacteria solution 3 serial dilutions (10 ^0, 10 ^2, 10 ⁴ dilution), each 0.1 ml DHL agar medium (Nissui Pharmaceutical Co., Nissui Plate DHL agar) to I inoculated. After culturing at 37 ° C for 24 hours, the number of colonies was measured.

Example 1. The following sterilization and bacterium removal experiments were conducted on the fibers of Examples 1 and 2 of the present invention and the comparative fiber obtained in the examples. Cut 0.25 g of the prepared fiber to a length of 3 cm, put it in a 10 ml glass test tube, add 8 ml of distilled water, plug it, and put it at 121 ℃.
Min autoclaved. After that, the supernatant was removed, 5 ml of the bacterial solution was added, and the mixture was shaken at room temperature and sampled at predetermined intervals. As a control, a test tube into which only the bacterial solution was injected was prepared and shaken in the same manner. After shaking for a predetermined time, the viable cell count was measured and the results shown in Table 1 were obtained.

From Table 1, it can be seen that the number of bacteria becomes zero in the example of the present invention in a short time, whereas in the comparative example having the same halogen atom having a similar chemical structure, the number of bacteria is not decreased and there is no effect.

In the comparative example, chloracetamide methylated fiber (water content 0.34 / PH 7.4, 0.34 / hydrochloric acid type) was used.

Example 2. It was investigated whether the bactericidal effect was due to elution from fibers. 0.25 g of the fiber of the present invention was cut into a length of 3 cm and placed in a 10 ml glass test tube, 8 ml of distilled water was added, and the plug was closed.
It was autoclaved at 21 ° C for 30 minutes. 1 ml of the supernatant was taken from this tube, 5 ml of the Escherichia coli solution described in Example 1 was added, and the mixture was shaken for 8 hours while sampling every predetermined time. As a control, a test tube injected with only the bacterial solution was also shaken in the same manner. After shaking, the viable cell count was measured and the results shown in Table 2 were obtained.

From Table 2, it was confirmed that the number of bacteria was the same as that of the control in the example of the present invention. Therefore, it is clear that the bacteria are not bacteria caused by the eluate from the fiber.

Example 3. Using the fiber of the present invention, the fungal treatment capacity was examined by repeated experiments. 0.25 g of the fiber of the present invention is cut into a length of 3 cm and 10 ml
It was put in a glass test tube, 8 ml of distilled water was put therein, and then the tube was capped and autoclaved at 121 ° C. for 30 minutes. After that, the supernatant was removed, 5 ml of the Escherichia coli solution described in Example 1 was added, and the mixture was shaken for 1 hour. After sampling the bacterial liquid, the bacterial liquid was discarded and 5 ml of the bacterial liquid having the same concentration was newly added, and the whole was repeated 4 times. The viable cell count was measured using the sampled sample and Table 3
The results shown in are obtained.

From Table 3, it can be seen that in the examples of the present invention, the effect is maintained with a small decrease in the bactericidal activity even when the experiment is repeated 4 times using the bacterial solution having a concentration of 24 × 10 ⁵ CFU / ml. Moreover, it could be performed without performing the reproducing operation.

Example 4 Bacteria and fungus removal experiments were carried out by changing the fungal species using the fiber of the present invention. The bacterial species include (1) Pseudomonas aeruginosa
monas aeruginosa ATCC 27853) (2) Serratia
marcescens ATCC 8100) (3) Klebsiella p.
neumoniae ATCC 27736) (4) Salmonella typhimurium (Salmon
ella typhimurium ATCC 13311) was used. 0.25 g of the fiber of the present invention is cut into a length of 3 cm and placed in a 10 ml glass test tube, 8 ml of distilled water is put therein, and then the tube is capped, and 121 ° C. 30
Min autoclaved. After that, the supernatant was removed, 5 ml of the bacterial solution was added, and the mixture was shaken at room temperature and sampled at predetermined intervals. As a control, a test tube into which only the bacterial solution was injected was prepared and shaken in the same manner. After shaking for a predetermined time, the viable cell count was measured and the results shown in Table 4 were obtained.

It can be seen from Table 4 that the examples of the present invention have a strong bactericidal effect against Gram-negative bacteria other than Escherichia coli.

Example 5 The fiber of the present invention was used to perform sterilization and removal experiments with Gram-positive bacteria. Staphylococcus aureus (Staphylococcus aureus ATCC 25923) was used as the bacterial species. 0.25 g of the fiber of the present invention is cut into a length of 3 cm and placed in a 10 ml glass test tube, 8 ml of distilled water is added, and then the cap is closed.
Autoclaved at 30 ° C for 30 minutes. After that, the supernatant was removed, 5 ml of the bacterial solution was added, and the mixture was shaken at room temperature and sampled at predetermined intervals. As a control, a test tube into which only the bacterial solution was injected was prepared and shaken in the same manner. After shaking for a predetermined time, the viable cell count was measured and the results shown in Table 5 were obtained. The medium used was tryptosoya agar medium (Nissui plate, Nissui Pharmaceutical Co., Ltd.).

It can be seen from Table 5 that the examples of the present invention have a strong bactericidal effect even against gram-positive Staphylococcus aureus.

EFFECTS OF THE INVENTION The present invention can reduce the level of bacterial contamination, control wound infection, and render the wound sterile. Moreover, since it does not elute from the material, it is non-toxic, non-sensitizing and non-irritating, and its antibacterial activity does not decrease during use, so it is safe and easy to handle.

Claims (1)

[Claims] 1. A sterilizer, which comprises contacting a bacterium or a medium containing the bacterium with a polymer having a functional group of the following general formula (1) as a substituent in a main chain or a side chain or a molded product thereof. How to remove bacteria. In the above formula, R ₁ and R ₂ represent a hydrogen atom or a lower alkyl group.