JPH0735994B2 - Recording method of migration pattern - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for recording an electrophoretic pattern in an electrophoretic device.

(Prior Art) As a recording format of an analysis result in an electrophoresis apparatus, there are one described in Japanese Utility Model Publication No. 56-9572 and one shown in FIG. The protein fractionation report 1 shown in FIG. 1 comprises a sample information print column 2 for a patient or the like, an analysis information column 3 and a migration pattern recording column 4. Analysis information column 3 is the inspection item column 3
a, normal value column 3b, judgment column 3c and measured value column 3d,
In the test item column 3a, TP (total protein), A / G (albumin to globulin ratio), ALB (albumin), α ₁ -G (α
₁ - _{globulin), α 2 -G (α 2} - globulins), beta
-G (β-globulin) and γ-G (γ-globulin) test items are pre-printed, and the normal value range 3b is printed with the pre-entered normal value range corresponding to each test item. It is becoming like this.

In the conventional recording method, the analysis result of each inspection item obtained by photometry of the electrophoretic image is printed at the corresponding position of each inspection item in the measurement value column 3d, and the analysis result is higher than the normal value range. Is printed at the position of the corresponding inspection item in the judgment column 3c, and "L" is printed at the time of the low value, and the migration pattern based on the photometric data of the electrophoretic image is recorded in the migration pattern recording field 4. I am trying to record.

However, in the electrophoretic device, basically, the electrophoretic pattern shows an important pathological condition, and therefore, by simply recording the electrophoretic pattern as in the past, which item is out of the normal value from this electrophoretic pattern. I don't know what. Therefore, when diagnosing with this kind of report,
I try to observe the migration pattern and the measured value of each item in correspondence, but it is very difficult to see the correspondence between the two in this way, and it is possible to overlook abnormal values or misidentify inspection items that show abnormal values. Therefore, it may lead to misdiagnosis.

(Object of the Invention) An object of the present invention is to solve the above-described problems and to provide a method for recording a migration pattern that allows easy recognition of abnormality of each component from the migration pattern.

(Summary of the Invention) The present invention stores the normal value range of each component of the migration pattern when photometrically measuring the electrophoretic image of the subject and recording the analysis value and migration pattern of each component in a predetermined report. Based on the comparison result, the process of comparing the measured value of each photometric component with the recorded normal value range, and the identification process performed for the analytical value higher than the normal value range and the lower than the normal value range. Along with selecting the identification process to be performed on the value analysis value, the identification mark that can be located in the recording area of the migration pattern corresponding to these abnormal analysis values and can identify whether the value is higher or lower than the normal value range. It is characterized in that it has a recording step and can confirm the pathological condition of each component of the migration pattern.

(Embodiment) FIG. 2 is a block diagram showing the configuration of the main part of an example of an electrophoretic device for carrying out the recording method of the present invention. Samples such as serum are applied to a support such as a cellulose acetate membrane with an applicator, and after electrophoresis for a predetermined time in an electrophoresis tank,
The photometry section is performed by dyeing, decolorizing and drying in the dyeing tank.
Transport to 11. In the photometric unit 11, the electrophoretic image formed on the support is photometrically scanned, the photoelectric conversion output is logarithmically amplified and converted into an absorbance value, and then the sampling rate determined by the analysis conditions such as the migration time. A / D conversion with CUP
Stored in memory 18 under control of 12.

On the other hand, the normal value range for each analysis item as shown in the table below is stored in the floppy disk 16 while being displayed on the CRT 15 by the preliminary keyboard 14.

The photometric data stored in the memory 13 is subjected to a smoothing process, which is a moving average process, and an auto-zero process is performed to remove the base floating amount due to the light amount, and then the necessary number of data points is cut out around the ALB peak point. Next, the cut-out data is subjected to the fraction point detection processing to obtain ALB, α ₁ -G, α
Divide into each fraction such as _2- G, β-G, γ-G, and calculate the measured value of each fraction%, A / G, etc.

In this example, the measured value obtained as described above is used by the printer 17
As shown in Fig. 3, the protein fraction report 1 in the same format as Fig. 1 is printed together with the normal value range and the judgment results of "L" and "H" by comparison with it, and the measured value of ALB. Auto-span is constantly recorded to record the electrophoretic pattern. In the electrophoretic pattern recording column 4, a "↑" is displayed when the abnormal value is a high value in the recording area of the item that is out of the normal value range together with the electrophoretic pattern. When the value is low, the "↓" mark 21 is printed. In this example, the mark 21 is printed on the lower side of the migration pattern at the center of the fraction points. In addition, this mark 21 calculates the print position and prints "↑" or "↓" at that position.
It can be printed by editing the print character in 17.

As described above, if the mark 21 having a function of discriminating whether the abnormal value is the high value or the low value is printed in the recording area of the item outside the normal value range together with the migration pattern, the migration pattern recording column Only by looking at 4, it is possible to easily recognize at a glance which item is out of the normal value. Therefore, it is possible to easily and intuitively recognize the migration pattern without corresponding measured values one by one, so that it is possible to effectively prevent overlooking of abnormal values and misdiagnosis due to misidentification of items showing abnormal values. .

It should be noted that the present invention is not limited to the above-mentioned examples, and many modifications and variations are possible. For example, the normal value range may be stored as the value of g / dl obtained by multiplying TP (total protein) by%, instead of the value of%, and the same determination may be performed. For items other than the desired item, the normal value range can be excluded from the determination by setting, for example, “0.0-0.0”. Further, although the mark 21 is printed on the lower side of the electrophoretic pattern in the above-described embodiment, it may be printed on the upper side as shown in FIG. 4A, and the printing position is divided point and divided point. It may be printed at the peak position instead of the center of. As shown in FIG. 4B, the mark 21 is located below the migration pattern when the abnormal value is high.
If the value is low, it may be printed on the upper side, or the fourth
The printing may be reversed as shown in FIG. In addition, mark 21
In addition to the arrow, as shown in FIG.
It is also possible to print by using "▼" in the same manner as in FIGS. 3 and 4A to 4C.

(Effect of the invention) As described above, according to the present invention, the mark is positioned and recorded in the recording area of the abnormal component corresponding to the electrophoretic pattern. Therefore, the electrophoretic pattern does not have to correspond to the analysis value one by one. Only by looking at, it is possible to intuitively and easily recognize how high or low the abnormal value of the analysis value is, together with the actual magnitude relationship with other components. Therefore, it is possible to effectively prevent oversight of an abnormal value and misdiagnosis due to misidentification of an item having an abnormal value.

[Brief description of drawings]

FIG. 1 is a diagram for explaining a conventional recording method, FIG. 2 is a block diagram showing the structure of an example of an electrophoretic device for carrying out the present invention, and FIG. 3 is recording by the electrophoretic device shown in FIG. FIGS. 4A to 4D are diagrams for explaining the method, and FIGS. 4A to 4D are diagrams for explaining a modified example of the present invention. 1 ... Protein fractionation report, 2 ... Sample information printing column 3 ... Analysis information column, 3a ... Test item column 3b ... Normal value column, 3c ... Judgment column 3d ... Measurement value column, 4 ... Electrophoresis pattern recording column 11 ... Photometry Parts, 12 ... CPU 13 ... Memory, 14 ... Keyboard 15 ... CRT 16 ... Flotpie disk 17 ... Printer, 21 ... Mark

Claims (2)

[Claims] 1. A step of storing a normal value range for each component of an electrophoretic pattern when photometrically measuring an electrophoretic image of a subject and recording an analysis value and an electrophoretic pattern of each component in a predetermined report; The step of comparing the analyzed value of each component with the stored normal value range, respectively, and based on the comparison result, the identification process performed for the analytical value higher than the normal value range and the analytical value lower than the normal value range. A step of selecting an identification process to be performed with respect to, and positioning in the recording area of the migration pattern corresponding to these abnormal analysis values, and recording an identification mark capable of identifying whether the value is higher or lower than the normal value range; A recording method of a migration pattern, characterized in that it has a constitution capable of confirming the pathological condition of each component of the migration pattern. 2. The identification process records the identification mark on the upper and lower sides of the migration pattern of the analysis value higher than the normal value range and the analysis value lower than the normal value range. A method for recording an electrophoretic pattern according to item 1.