JPH06100534B2 - Recording method of migration pattern - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for recording an electrophoretic pattern in an electrophoretic device.

(Prior Art) In an electrophoretic device, a sample such as serum is applied to a support such as a cellulose acetate film by an applicator, allowed to migrate in a migration tank for a predetermined time, and then stained, decolorized and dried in a staining tank. After that, it is conveyed to a densitometer, where the electrophoretic image is photometrically scanned.

FIG. 1 shows the configuration of the main part of a densitometer in such an electrophoretic device. The support 1, which has been dyed, decolorized and dried in the dyeing tank, is conveyed by the feed roller 2 to the measurement unit 4 that houses the decalin 3, where it is photometrically scanned by the photometric device 5 and then ejected by the paper ejection roller 6. To be done. The photometric device 5 is configured to receive the light from the light source 5a that passes through the support by the light receiving element 5b, and the photometric device 5 having the light source 5a and the light receiving element 5b is used as shown in FIG. Scanning direction b orthogonal to the transport direction a
The electrophoretic image 7 formed on the support 1 is photometrically scanned by moving the electrophoretic image 7 onto the support 1.

As described above, the photometric data obtained by photometrically scanning the electrophoretic image 7 in the densitometer is sampled at a predetermined cycle, and the measured value of each inspection item is calculated based on the sampled data to determine the predetermined value in the printer. As shown in Fig. 3, when the sample is human serum, the auto-spanning migration pattern printed on the report shows prealbumin 8, albumin (ALb) 9, α
₁ -globulin (α ₁ -G) 10, α ₂ -globulin (α
Electrophoresis pattern 14 having components of _2- G) 11, β-globulin (β-G) 12 and γ-globulin (γ-G) 13
Are recorded together with the report.

Thus, the report on the electrophoresis apparatus is different from the report used in general biochemical analysis in that the migration pattern of the fraction is also recorded.

On the other hand, in general biochemical analysis, user needs for preparing reports are diversifying with the spread of devices. Therefore, for example, in JP-A-56-149636,
A printer control method has been proposed in which required data can be printed on a report of various sizes and formats without changing the hardware for creating the report and its program.

This control method can be effectively applied to the preparation of a report in an electrophoretic device, whereby the printing position of measured values and the recording position of the electrophoretic pattern can be arbitrarily changed.

However, even if the printing position of the measurement value and the recording position of the electrophoretic pattern can be changed in this way, in the conventional electrophoretic device, the size of the electrophoretic pattern to be recorded is fixed,
The user could not change the size freely. For this reason, there is a risk of misdiagnosis when diagnosing a pathological condition from the migration pattern, and development of an apparatus that can obtain a migration pattern that enables accurate diagnosis is desired.

(Object of the Invention) The present invention has been made in order to meet the above-mentioned demand,
It is an object of the present invention to provide a migration pattern recording method capable of recording a migration pattern of a desired size in whole and / or part thereof.

(Summary of the Invention) According to the present invention, a step of storing photometric data obtained by photometrically measuring an electrophoretic image in a predetermined storage means, and displaying an entire electrophoretic pattern of a reference size based on the photometric data on a CRT. And a step of cutting out the photometric data into a region for each protein fraction, and a longitudinal magnification for all and / or a part of the protein fraction of the migration pattern based on the migration pattern of the displayed standard size and And / or the step of selectively inputting the lateral magnification, the step of displaying the input command content on the CRT and storing it in the storage means, and the recording operation of the printer based on the content stored in the storage means. It is characterized in that it comprises a step of recording the electrophoretic pattern of the standard size and the electrophoretic pattern which has been vertically and / or horizontally multiplied on the same recording paper by controlling. .

(Embodiment) FIG. 4 is a block diagram showing a configuration of a main part of an example of an electrophoretic device for carrying out the present invention. The electrophoretic image formed on the support 21 is photometrically scanned at a constant speed by a photometric device having a light source 22 and a light receiving element 23 in the densitometer, and the measurement data is logarithmically amplified by a logarithmic amplifier 24 and converted into absorbance. After that, the A / D converter 25 performs sampling at a sampling rate determined by analysis conditions such as migration time, converts the signal into, for example, a 12-bit digital signal, and stores the digital signal in the memory 27 under the control of the CPU 26. The A / D converted photometric data is subjected to smoothing processing which is a moving average processing under the control of the CPU 26,
After Eat-zero processing to remove the base floating due to the light intensity, the required number of data points are cut out based on the ALb peak position, and the fractional point detection processing is performed based on the cut out data to obtain ALb, α _1- G, α _2- G, β-G, γ-G fraction%, A / D (albumin to globulin ratio) measured values are calculated and stored in the floppy disk 28, and data for recording migration patterns As a result of the auto span processing of the cut out data, for example, the maximum value is 12
It is normalized so as to have the maximum value of the bits and is similarly stored in the floppy disk 28.

In this example, the measured value stored in the floppy disk 28 is printed in a predetermined column of the protein fractionation report by the printer 29, and all and / or a part of the standard size migration pattern based on the normalized data is printed. In order to record the desired size in the predetermined column of the protein fractionation report, the migration pattern based on the above normalized data is displayed on the CRT31 in a standard size prior to the recording, and the display is performed. A required display command is input while displaying it on the CRT 31 by the keyboard 30 based on the migration pattern of the standard size, and stored in the floppy disk 28. The printer 29 uses an image printer or a graphic printer. In this example, as the printer 29, a heat-sensitive friction feed type 8-bit printer as shown in FIG. 5 is used, and the protein fractionation report 32 has a TOF (TOP OF FORM) mark 23.
Use the format shown in the figure.

In FIG. 5, the printer 29 is the TOF of the protein fractionation report 32.
A sensor 34 for detecting the mark 33, a paper feed roller 35, and a head 36 composed of a dot row of heaters integrated at 6 dots / mm.
By controlling ON / OFF of each dot, required data is recorded in a predetermined column of the protein fractionation report 32. The printer 29 starts paper feeding when the TOF code is input through the CPU 26, and temporarily stops when the sensor 34 detects the TOF mark 33. At this stop position, the head 36 corresponds to the first line of the report 32, and thereafter, the CPU 26 controls recording of required data and paper feeding. But,
The relative position between the head 36 and the protein fractionation report 32 is determined by the sensor 34
Deviation between the head and head 36, printing error in the TOF mark 33 and recording field, or lot exchange of the report 32, which causes the required data to be recorded accurately in the prescribed field of the report 32. Sometimes you can't. Therefore, in this embodiment, in order to correct such a shift, the TOF
The correction data is also stored in advance in the floppy disk 28 as a display command.

An example of the display command when the protein fraction report 32 in the printer 29 described above is used is shown in the table below.

That is, in the case of the above table, the report 32 is 5 after the TOF operation.
After dots are sent and the relative position with the head 36 is adjusted,
The patient name is recorded on the first line, and thereafter, required data is similarly edited and recorded in a predetermined column. In the above table, the electrophoretic pattern magnification of 10/83 is multiplied by the normalized photometric data, and the electrophoretic pattern recording column 32a of the protein fractionation report 32 shown in FIG. It is the vertical magnification when recording the pattern P ₀ . Therefore, by setting the vertical magnification to 10/83 or less, it is possible to record a migration pattern P ₁ (broken line) smaller than the reference migration pattern P ₀ (solid line) as shown in FIG. , 10/83 or more, it is possible to record a migration pattern P ₂ (dashed line) larger than P ₀ .

The method of recording the migration pattern will be described below. When recording the migration pattern, first, a floppy disk
Normalized photometric data stored in 28 is stored in memory 27
, And each data is multiplied by the magnification input by the display command. Now, the four normalized photometric data D1
As a result of multiplying D4 to D4, it is assumed that D1 = 0, D2 = 3, D3 = 2, D4 = 0. Further, it is assumed that when recording the migration pattern, the head 36 of the printer 29 is stopped at the position 4 when the reference point is 0, as shown in FIG. To record the electrophoretic pattern, each dot is turned on and off while the paper is fed one pitch at a time. When the head 36 comes to the position marked with ◎ in FIG. 8, the dots corresponding to dots a to d are turned on. In addition to this, an interpolation process is required to turn on the corresponding dot at the mark position in order to obtain a continuous waveform. Such data interpolation processing can be performed by various known methods. The interpolation process is completed and the memory
When the data interpolated in 27 is complete, the dots a to d are controlled while feeding the paper one dot line at a time to record the electrophoretic pattern. In this case, however, since there is no data to be printed at the head position 4, 1 with dots a to d turned off
Send the dot line paper. Next, at head position 3, dot a,
b and c, at head position 2, dots ac and d,
The dots a and d are turned on at the head position 1, and the dots a and d are turned on at the head position 0, that is, at the reference point, to obtain a migration pattern of a continuous waveform.

In this way, the specified resolution is multiplied by the photometric data stored in the floppy disk 28, and the data is interpolated to form a continuous waveform, so that the maximum resolution of the printer 29 is always maintained for any magnification. The migration pattern can be recorded with.

FIG. 9 is a flow chart showing the outline of the operation of this embodiment, and the description thereof is omitted because it has already been described.

As described above, in the present embodiment, the display command is input, and the printer 29 is controlled based on the command to record the character data such as the patient name and the measured value in predetermined fields of the protein fractionation report 32, respectively, The migration pattern recording column 32a of the report 32 records a migration pattern of a desired size.
The electrophoretic pattern to be recorded is not limited to the vertical magnification described above, but the entire electrophoretic pattern can be recorded by arbitrarily designating the horizontal magnification and both magnifications. Also, FIG. 10A
It is also possible to record only a part of the electrophoretic pattern with a reference size, or to enlarge or reduce the size as shown in FIG. It is also possible to record at different magnifications of the area, or to display together with the reference migration pattern P ₀ as shown in FIG. Although the friction field type printer is used in the above-described embodiment, a tractor feed type printer may be used.

(Effects of the Invention) As described above, according to the present invention, a migration pattern corresponding to a region of an arbitrary protein fraction is changed to an easily observable magnification based on the entire migration pattern displayed on a CRT. Since the electrophoretic pattern of the standard size can be recorded together,
It is possible to create a record that enables an accurate diagnosis according to the condition.

[Brief description of drawings]

FIG. 1 is a diagram showing a configuration of a main part of a densitometer, FIG. 2 is a diagram for explaining a method for scanning an electrophoretic image, FIG. 3 is a diagram showing an example of electrophoretic pattern, and FIG. FIG. 5 is a block diagram showing a configuration of an example of a main part of the electrophoretic device to be implemented, FIG. 5 is a perspective view showing a configuration of a main part of the example of the printer shown in FIG. 4, and FIG. 6 is an example of a protein fractionation report. FIG. 7 is a plan view showing an electrophoretic pattern recording mode according to the present invention, FIG. 8 is a view for explaining a control example of a printer, and FIG. 9 is data in the electrophoretic apparatus shown in FIG. FIG. 10 is a flowchart for explaining the process, and FIGS. 10A, 10B, and 10C are diagrams showing another recording mode of the migration pattern according to the present invention. 21 ... Support, 22 ... Light source 23 ... Photodetector, 24 ... Logarithmic amplifier 25 ... A / D converter, 26 ... CPU 27 ... Memory, 28 ... Floppy disk 29 ... Printer, 30 ...... Keyboard 31 …… CRT, 32 …… Protein Fractionation Report 33 …… TOF mark, 34 …… Sensor 35 …… Paper feed roller, 36 …… Head

Claims (1)

[Claims] 1. A step of storing photometric data obtained by photometrically measuring an electrophoretic image in a predetermined storage means, and a step of displaying an entire electrophoretic pattern of a reference size based on the photometric data on a CRT, A step of cutting out the photometric data into a region for each protein fraction, and a longitudinal magnification and / or a lateral magnification for the whole and / or a part of the protein fraction of the electrophoretic pattern based on the electrophoretic pattern of the displayed standard size. By selectively inputting, the step of displaying the input command content on the CRT and storing it in the storage means, and controlling the recording operation of the printer based on the content stored in the storage means. And recording a migration pattern of a standard size and a migration pattern vertically and / or horizontally multiplied on the same recording paper. Law.