JPH0735995B2 - Recording method of migration pattern - Google Patents

Description

TECHNICAL FIELD The present invention relates to a method for recording an electrophoretic pattern in an electrophoretic device.

(Prior Art) In an electrophoretic device, a sample such as serum is applied to a support such as a cellulose acetate film by an applicator, allowed to migrate in a migration tank for a predetermined time, and then stained, decolorized and dried in a staining tank. After that, it is conveyed to a densitometer, where the electrophoretic image is photometrically scanned.

FIG. 1 shows the configuration of the main part of a densitometer in such an electrophoretic device. The support 1 that has been dyed, decolorized and dried in the dyeing tank is conveyed by the feed roller 2 to the photometric unit 4 that houses the decalin 3, where it is photometrically scanned by the photometric device 5 and then ejected by the paper ejection roller 6. To be done. The photometric device 5 is configured to receive the light from the light source 5a that passes through the support by the light receiving element 5b, and the photometric device 5 having the light source 5a and the light receiving element 5b is used as shown in FIG. Scanning direction b orthogonal to the transport direction a
The electrophoretic image 7 formed on the support 1 is photometrically scanned by moving the electrophoretic image 7 onto the support 1.

As described above, the photometric data obtained by photometrically scanning the electrophoretic image 7 in the densitometer is sampled at a predetermined cycle, and the measured values and the like of each inspection item are calculated based on the sampled data. A predetermined report is printed on the printer, and the electrophoretic pattern of the electrophoretic image 7 shows, for example, when the sample is human serum, as shown in FIG.
b) 9, components of α ₁ -globulin (α ₁ -G) 10, α ₂ -globulin (α ₂ -G) 11, β-globulin (β-G) 12 and γ-globulin (γ-G) 13 The migration pattern 14 with is recorded together in the report.

However, in recording the migration pattern 14, conventionally, sequential data from a mechanical scanning start point in the densitometer or sequential data from a time point when a predetermined distance is moved from the mechanical scanning start point is used. Therefore, when the support 1 skews in the densitometer, the migration pattern 14 recorded in the report shifts as shown in FIG. 4B as compared with the one without skewing shown in FIG. 4A. ,
It may be very difficult to see and the necessary data may not be recorded.

On the other hand, as an electrophoresis device, in order to make the processing speed variable or to support the analysis of various samples,
Some have variable migration times. In such a device, since the migration development length is proportional to the migration time, the sampling cycle of the photometric data is also set long when the migration time is set long and short when the migration time is set short,
The photometric data is sampled at a sampling cycle corresponding to the migration time, and the measured value and migration pattern are recorded in a predetermined report in the same manner as described above based on the sampled data. However, in such a device, since the scanning range of the densitometer is constant regardless of the migration time, the number of data points due to sampling differs depending on the migration time, and the shorter the migration time, that is, the shorter the sampling cycle, the larger the number of data points. Therefore, irrespective of the migration time, if the sampled data is processed in the same manner as described above to record the migration pattern, the migration time causes a shift in the migration pattern as in FIGS. Data may not be recorded.

(Object of the invention) An object of the present invention is to solve the above-mentioned problems and to fix a fixed point in which a reference point corresponding to a predetermined position in an electrophoretic pattern where an electrophoretic pattern is to be recorded and a predetermined position of a report are matched. It is intended to provide a method for recording a migration pattern that can always be accurately recorded in a state.

(Summary of the Invention) A method for recording an electrophoretic pattern according to the present invention comprises a step of photometrically scanning a support having an electrophoretic image to obtain a plurality of photometric data sequentially along a certain direction of the electrophoretic image, Among the data, a step of detecting a photometric point determined according to the maximum value as a reference point, a step of extracting a predetermined number of photometric data corresponding to a predetermined protein fraction both before and after the reference point, and a report, A step of presetting a recording range on which the electrophoretic pattern of a predetermined number of photometric data fits and a predetermined position corresponding to the reference point in the recording range; and the detected reference point and the predetermined number of the report. And a step of recording the photometric data extracted so as to match the position as a migration pattern.

Furthermore, the migration pattern recording method of the present invention comprises a step of sampling data obtained by photometrically scanning a support having an electrophoretic image at a predetermined sampling period, and the sampling photometric data and a predetermined threshold value. In comparison, a step of detecting the electrophoretic development length of the electrophoretic image that exceeds the threshold value and a step of determining a sampling period for obtaining the detected electrophoretic development length at a constant number of photometric points, A step of sampling photometric data corresponding to the case where the support is photometrically scanned by changing the sampling period, and of the photometric data sampled at the determined sampling period, a photometric point determined according to the maximum value is used as a reference point. Extracting a predetermined number of photometric data corresponding to a predetermined protein fraction both before and after the detecting step and before and after the reference point And a step of presetting a recording range in which a migration pattern based on a predetermined number of photometric data fits on the report and a predetermined position corresponding to the front and rear reference points in the recording range, and the detected reference point And a step of recording the photometric data extracted so as to match the predetermined position of the report as a migration pattern.

(Embodiment) FIG. 5 is a block diagram showing a configuration of a main part of an example of an electrophoretic apparatus for carrying out the recording method of the present invention. In this example, the protein fraction of human serum is analyzed, and the electrophoretic image of human serum formed on the support 21 is a constant speed by a photometric device having a light source 22 and a light receiving element 23 in the densitometer, for example, Photometric scanning is performed at 8 mm / sec. The photoelectric conversion output obtained from the light receiving element 23 by this photometric scanning is logarithmically amplified by the logarithmic amplifier 24 and converted into absorbance, and then the sampling rate (determined by the analysis conditions such as the migration time in the A / D converter 25 ( For example, it is sampled in synchronization with a clock corresponding to 12 msec), converted into a digital signal, and stored in the memory 27 under the control of the CPU 26.

In this example, the photometric data stored in the memory 27 is subjected to a smoothing process which is a moving average process and an auto-zero process for removing a base floating amount due to the light amount, and then 100 points on the prealbumin side around the ALb peak point. , 250 points of data are extracted on the γ-G side.

In extracting this data, as shown in FIG.
First, the maximum value (D _M ) in all the data on the memory 27 is detected, and then the peak position P _M of 1/16 or more of D _M is detected from the prealbumin side. Here, 1/16 to remove the peaks of prealbumin, as D _M is P _M be other components not ALb is detected at a peak position of ALb, empirically by various diseases Since it was set, it is not particularly fixed. As a result, when the peak position P _{M of} ALb is detected as shown in FIG. 7, a total of 350 points of 100 points on the prealbumin side and 250 points on the γ-G side from this P _M are extracted from the memory 27. The floppy disk shown in FIG.
Remember in 28. If 100 or 250 points of data do not exist from P _M , data of points that do not exist at the end values are created, and 100 and 250 points of data are obtained from actual P _M.

Thus, based on the ALb peak position P _M , the sequential 350
After the point data is extracted, the fraction point detection process, the fraction% and the A / G (albumin to globulin ratio) calculation process are performed based on the extracted data, and the result is stored in the floppy disk 28. Then, by the command of CPU26,
Measured values such as calculated fraction% and A / G can be
As shown in FIG. 8, the electrophoretic pattern 31 is printed in a predetermined column of the protein fractionation report 30 and the electrophoretic pattern 31 based on 350 points of data extracted in a predetermined range of the electrophoretic pattern recording column of the protein fractionation report 30 is displayed. , The maximum value D _M is standardized, that is, auto-span is recorded.

In this way, the support 21 in the densitometer
Even if the sample is skewed, the migration pattern 31 based on the extracted 350 points of data is recorded in the predetermined range of the migration pattern recording column of the protein fractionation report 30 to detect the albumin peak position (reference point) and the protein. It is possible to record the fractional report 30 in a state in which it coincides with a predetermined position, and therefore, it is possible to always accurately record the migration pattern in a predetermined position and in a constant state.

In another embodiment of the present invention, even when the migration time is changed according to the processing speed and the type of sample, the migration pattern is always accurately recorded at a predetermined position in a constant state. Therefore, in the present embodiment, first, the electrophoretic image is pre-scanned and the photometric data is sampled at a reference sampling rate, for example, 12 msec, and the data exceeding the predetermined threshold value is obtained from the scan data as shown in FIG. , The migration development length L is obtained. Next, the electrophoretic image is rescanned, the scan data is sampled at a sampling rate according to the electrophoretic development length L obtained by the prescan, and the same electrophoretic image as the above-described embodiment is processed to determine a predetermined electrophoretic image. A predetermined range of data is extracted based on the reference points, and required measurement values and migration patterns are recorded based on the extracted data.

Here, the sampling rate R according to the migration development length L
Is calculated and determined by the following formula, for example, and the set value of the programmable timer is changed accordingly to generate a corresponding sampling clock.

R = L / (mxs) In the above equation, m is an arbitrary number of sampling points included in the migration expansion length L, for example, as in the above-mentioned embodiment.
When extracting 350 points of data, set m to 250. Further, s is a scan speed, which is usually 8 mm / sec.

An example of the sampling rate R determined according to the migration development length L in this manner is shown in the table below.

Therefore, according to the present embodiment, even if there is a difference in the electrophoretic development length L, the electrophoretic pattern can always be accurately recorded at a predetermined position in a constant state, as in the above-described embodiments.

It should be noted that the present invention is not limited to the above-described embodiments, and many modifications and variations are possible. For example,
Although the peak position of ALb is used as the reference point in the above-described embodiment, the peak position of an arbitrary component, for example, γ-G or α ₂ -G.
The peak position of can be used as the reference point. In such a case, the maximum value D _M as in the above-described embodiment is detected, and the processing of detecting the peak position of 1/16 or more of the D _M from the prealbumin side is not performed, but from the scanning direction. Alternatively, the reference point can be specified by detecting a predetermined peak position from the opposite direction or a predetermined peak position from both directions. Further, such a reference point specifying method can be applied to the above-described embodiment. Further, the number of data to be extracted based on the reference point can be set arbitrarily, and the number of data to be extracted on both sides of the reference point in the scanning direction can also be set arbitrarily according to the position of the reference point.

(Effects of the Invention) As described above, according to the present invention, the migration pattern is not affected by skewing of the support, migration time, etc. It is possible to always record accurately in a fixed state in which the reference point corresponding to the predetermined position and the predetermined position of the report are matched. Therefore, it is easy to see and it is possible to effectively prevent erroneous diagnosis at the time of shaking due to the migration pattern.

[Brief description of drawings]

FIG. 1 is a diagram showing a configuration of a main part of a densitometer, FIG. 2 is a diagram showing a scanning direction of an electrophoretic image, FIG. 3 is a diagram showing an example of a migration pattern, and FIGS. 4A and 4B are conventional migrations. FIG. 5 is a diagram for explaining a defect in a pattern recording method, FIG. 5 is a block diagram showing a configuration of a main part of an example of an electrophoretic apparatus for carrying out the recording method of the present invention, FIGS. 6, 7, and 8. FIG. 9 is a diagram for explaining an example of the recording method of the present invention, and FIG. 9 is a diagram for explaining another embodiment of the present invention. 21 ... Support, 22 ... Light source 23 ... Light receiving element, 24 ... Logarithmic amplifier 25 ... A / D converter, 26 ... CPU 27 ... Memory, 28 ... Floppy disk 29 ... Printer, 30 ... Protein fractionation report 31 ... Migration pattern

Claims (2)

[Claims] 1. A step of obtaining a plurality of sequential photometric data along a fixed direction of the electrophoretic image by photometrically scanning a support having an electrophoretic image, and determining the maximum value of the photometric data. The step of detecting the photometric point as a reference point, the step of extracting a predetermined number of photometric data corresponding to the predetermined protein fractions both before and after the reference point, and on the report by the predetermined number of photometric data A step of presetting a recording range in which the electrophoretic pattern fits and a predetermined position corresponding to the reference point in the recording range; and photometry extracted so that the detected reference point and the predetermined position of the report coincide with each other. And a step of recording data as an electrophoretic pattern. 2. A step of sampling data obtained by photometrically scanning a support having an electrophoretic image at a predetermined sampling period, and comparing the sampled photometric data with a predetermined threshold value to obtain the threshold value. A step of detecting the electrophoretic development length of the electrophoretic image that exceeds the above, a step of determining a sampling cycle for obtaining the detected electrophoretic development length at a constant number of photometric points, and changing to the determined sampling cycle A step of sampling photometric data corresponding to a case where the support is photometrically scanned, a step of detecting, as a reference point, a photometric point determined according to a maximum value of the photometric data sampled at the determined sampling period, A step of extracting a predetermined number of photometric data corresponding to a predetermined protein fraction before and after each point, and a predetermined number on the report. A step of presetting a recording range in which the migration pattern based on the photometric data fits and a predetermined position corresponding to the front and rear reference points in the recording range, and the detected reference point and the predetermined position of the report being matched. And a step of recording the extracted photometric data as an electrophoretic pattern.