JPH0745593B2 - Reinforcing method of gel polymer - Google Patents
Reinforcing method of gel polymerInfo
- Publication number
- JPH0745593B2 JPH0745593B2 JP61181457A JP18145786A JPH0745593B2 JP H0745593 B2 JPH0745593 B2 JP H0745593B2 JP 61181457 A JP61181457 A JP 61181457A JP 18145786 A JP18145786 A JP 18145786A JP H0745593 B2 JPH0745593 B2 JP H0745593B2
- Authority
- JP
- Japan
- Prior art keywords
- gel
- substance
- polymer
- reinforcing
- polymer substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920000642 polymer Polymers 0.000 title claims description 23
- 238000000034 method Methods 0.000 title claims description 12
- 230000003014 reinforcing effect Effects 0.000 title claims description 8
- 239000000126 substance Substances 0.000 claims description 36
- 244000005700 microbiome Species 0.000 claims description 8
- 108010093096 Immobilized Enzymes Proteins 0.000 claims description 7
- 239000001913 cellulose Substances 0.000 claims description 7
- 229920002678 cellulose Polymers 0.000 claims description 7
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical group NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- 229920001817 Agar Polymers 0.000 claims description 2
- 229910018072 Al 2 O 3 Inorganic materials 0.000 claims description 2
- 239000005909 Kieselgur Substances 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 229910004298 SiO 2 Inorganic materials 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- ZNOZWUKQPJXOIG-XSBHQQIPSA-L [(2r,3s,4r,5r,6s)-6-[[(1r,3s,4r,5r,8s)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-4-[[(1r,3r,4r,5r,8s)-8-[(2s,3r,4r,5r,6r)-3,4-dihydroxy-6-(hydroxymethyl)-5-sulfonatooxyoxan-2-yl]oxy-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-3-yl]oxy]-5-hydroxy-2-( Chemical compound O[C@@H]1[C@@H](O)[C@@H](OS([O-])(=O)=O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H]2OC[C@H]1O[C@H](O[C@H]1[C@H]([C@@H](CO)O[C@@H](O[C@@H]3[C@@H]4OC[C@H]3O[C@H](O)[C@@H]4O)[C@@H]1O)OS([O-])(=O)=O)[C@@H]2O ZNOZWUKQPJXOIG-XSBHQQIPSA-L 0.000 claims description 2
- 239000004927 clay Substances 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 229920001059 synthetic polymer Polymers 0.000 claims description 2
- 230000002787 reinforcement Effects 0.000 claims 1
- 239000000499 gel Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 239000000440 bentonite Substances 0.000 description 3
- 229910000278 bentonite Inorganic materials 0.000 description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 3
- 239000003505 polymerization initiator Substances 0.000 description 3
- MTPJEFOSTIKRSS-UHFFFAOYSA-N 3-(dimethylamino)propanenitrile Chemical compound CN(C)CCC#N MTPJEFOSTIKRSS-UHFFFAOYSA-N 0.000 description 2
- 230000000274 adsorptive effect Effects 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000010526 radical polymerization reaction Methods 0.000 description 1
- 239000012779 reinforcing material Substances 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、固定化微生物又は固定化酵素の担体を構成す
る親水性ゲル状高分子物質の補強方法に関するものであ
る。TECHNICAL FIELD The present invention relates to a method for reinforcing a hydrophilic gel polymer substance that constitutes a carrier for immobilized microorganisms or immobilized enzymes.
一般に水を含むゲル状高分子物質は力学的強度が弱く、
特にゲル中のポリマー密度が低くなると、その強度は極
端に弱くなり、様々な用途での使用に耐えない。In general, gel-like polymeric substances containing water have weak mechanical strength,
In particular, when the polymer density in the gel becomes low, its strength becomes extremely weak and it cannot withstand use in various applications.
そこで、従来は、このようなゲル状高分子物質を補強す
る方法としては、適当な強度を持つ固体上にゲル状高分
子物質を付着させるか、固体をゲル内に包括させる方法
が知られている。Therefore, conventionally, as a method of reinforcing such a gel-like polymer substance, a method of attaching the gel-like polymer substance onto a solid having appropriate strength or encapsulating the solid in a gel is known. There is.
然し、これらの方法は、ゲル自体の強度を上げるのでは
なく、ゲルの骨組として補強材を用いるため、使用し得
るゲルの形状，大きさに限界があり、ゲルを数cm以下の
球状，柱状又はペレット状等の形で使用した場合に、こ
の方法を利用することは困難である。However, since these methods do not increase the strength of the gel itself but use a reinforcing material as a framework of the gel, there is a limit to the shape and size of the gel that can be used. Alternatively, it is difficult to use this method when used in the form of pellets or the like.
本発明は斯る従来の問題点を解決するために為されたも
ので、その目的は、固定化微生物又は固定化酵素の担体
を構成するゲル状物質自体に弾力性を持たせることによ
ってゲル状高分子物質の強度を上げ、壊れ難くすること
にある。The present invention has been made to solve such conventional problems, and its purpose is to provide a gel-like substance by imparting elasticity to the gel-like substance itself that constitutes the carrier of the immobilized microorganism or the immobilized enzyme. The purpose is to increase the strength of polymeric materials and make them difficult to break.
本発明に係るゲル状高分子物質の補強方法は、固定化微
生物又は固定化酵素の担体を構成するゲル状高分子物質
内に、吸着性の強い物質又は微細繊維状物質を混入する
ものである。The method for reinforcing a gel-like polymer substance according to the present invention is to mix a highly adsorptive substance or a fine fibrous substance into the gel-like polymer substance forming the carrier of the immobilized microorganism or the immobilized enzyme. .
本発明に於ける固定化微生物又は固定化酵素の担体を構
成するゲル状高分子物質としては、アクリルアミド、ア
ルギン酸ナトリウム、ポリビニルアルコール、寒天ゲ
ル、κ−カラギーナン、又はこれらの高分子を主鎖に持
つ高分子等がある。Examples of the gel-like polymer substance constituting the carrier of the immobilized microorganism or immobilized enzyme in the present invention include acrylamide, sodium alginate, polyvinyl alcohol, agar gel, κ-carrageenan, or those polymers in the main chain. There are macromolecules.
又、本発明に於ける吸着性の強い物質としては、SiO2、
Al2O3、活性炭、又は例えばベントナイトの如くこれら
の物質を主成分とするもの、或いはケイソウ土、白土等
天然に存在するもの等の粉体がある。そして、吸着性の
強い物質は、水に懸濁し易い微粉末であることが望まし
い。Further, as the substance having a strong adsorptivity in the present invention, SiO 2 ,
There are powders of Al 2 O 3 , activated carbon, or those containing these substances as a main component such as bentonite, or naturally occurring substances such as diatomaceous earth and clay. The substance having strong adsorptivity is preferably a fine powder that is easily suspended in water.
更に、微細繊維状物質としては、セルロース等の高分子
或いは結晶性の高い合成高分子等がある。Further, as the fine fibrous substance, there is a polymer such as cellulose or a synthetic polymer having high crystallinity.
本発明に於ては、固定化微生物又は固定化酵素の担体を
構成するゲル状高分子物質内に、吸着性の強い物質又は
微細繊維状物質を混入したから、ゲル自体に弾力性に富
み破断限界の高い性質を持たせることができる。In the present invention, the gel-like polymer substance constituting the carrier of the immobilized microorganism or the immobilized enzyme is mixed with a substance having a strong adsorptivity or a fine fibrous substance, so that the gel itself is highly elastic and rupturable. It can have a high limit property.
以下、本発明を実施例に基づいて詳述する。 Hereinafter, the present invention will be described in detail based on examples.
実施例１ 先ず、直径44mm,高さ20mmの円筒を用意し、この中に第
一表に示す如き配合に従ってアクリルアミドモノマー
（AAと略称する）と、架橋剤として用いるN,N′−メチ
レンビスアクリルアミド（BISと略称する）の混合液
に、吸着性の粉体（ベントナイト）又は微細繊維状物質
（セルロース）の懸濁液を加え、更に過硫酸カリウム
（K2S2O8）にジメチルアミノプロピオニトリル（DMAPN
と略称する）を加えた重合開始剤を混合して重合した。Example 1 First, a cylinder having a diameter of 44 mm and a height of 20 mm was prepared, and an acrylamide monomer (abbreviated as AA) and N, N′-methylenebisacrylamide used as a cross-linking agent according to the formulation shown in Table 1 were prepared therein. To a mixed solution of BIS (abbreviated as BIS), a suspension of adsorptive powder (bentonite) or a fine fibrous substance (cellulose) was added, and then potassium persulfate (K 2 S 2 O 8 ) was added to the mixture. Pionitrile (DMAPN
It is abbreviated) and a polymerization initiator was added to perform polymerization.
そして、上記円筒から取り出して直径44mm,高さ15mmの
円柱を５種類作った。Then, five types of cylinders having a diameter of 44 mm and a height of 15 mm were made by taking them out from the cylinder.
比較例１ 上記実施例１と同様に、直径44mm,高さ20mmの円筒を用
意し、この中に第一表に示す如き配合に従ってアクリル
アミドモノマーと、架橋剤として用いるN,N′−メチレ
ンビスアクリルアミドの混合液に、過硫酸カリウムにジ
メチルアミノプロピオニトリルを加えた重合開始剤を混
合して重合した。Comparative Example 1 A cylinder having a diameter of 44 mm and a height of 20 mm was prepared in the same manner as in Example 1 described above, in which an acrylamide monomer and N, N'-methylenebisacrylamide used as a cross-linking agent according to the formulation shown in Table 1 were used. A polymerization initiator obtained by adding dimethylaminopropionitrile to potassium persulfate was mixed with the mixed solution of 1 to polymerize.
そして、上記円筒から取り出して直径44mm,高さ15mmの
円柱を５種類作った。Then, five types of cylinders having a diameter of 44 mm and a height of 15 mm were made by taking them out from the cylinder.
以上のようにして作成した20個の円柱について、それぞ
れ上方から板を介して均一に荷重を掛けてこれらの破壊
強度（一軸圧縮時の破壊荷重）を測定した。その結果を
第１図に示す。A load was uniformly applied from above to each of the 20 cylinders created as described above through a plate, and their fracture strength (the fracture load during uniaxial compression) was measured. The results are shown in FIG.
第１図から明らかな如く、実施例１で作られた15種類の
円柱は、何れも比較例１で作られた５種類の円柱に比べ
て破壊強度があることが確認された。As is clear from FIG. 1, it was confirmed that the 15 types of cylinders produced in Example 1 all had a fracture strength as compared with the 5 types of columns produced in Comparative Example 1.
特に、アクリルアミド濃度が４％,5％及び7.5％に於て
も優れた効果を発揮していることは、毒性の点でアクリ
ルアミド濃度を10％以下にすることが望まれている微生
物又は酵素の固定化担体にとっては有効である。In particular, the excellent effect is exhibited even when the acrylamide concentration is 4%, 5%, and 7.5%. It is effective for the immobilization carrier.
実施例２ 実施例１に於て最も破壊強度が低かったセルロース3.5
％混入の組成物にて、略7mmφの球状物を200個作った。Example 2 Cellulose 3.5 having the lowest breaking strength in Example 1
%, A spherical composition having a diameter of about 7 mm was prepared.
比較例２ 比較例１に於てアクリルアミド濃度が7.5％の配合組成
物にて、略7mmφの球状物を200個作った。Comparative Example 2 With the compounded composition of Comparative Example 1 having an acrylamide concentration of 7.5%, 200 spherical particles having a diameter of about 7 mm were prepared.
これらをそれぞれ同一の水槽に入れて邪魔板付きの撹拌
器にて撹拌し、撹拌時間に対する破壊ゲルの個数を測定
した。その結果を第２図に示す。These were placed in the same water tank and stirred with a stirrer with a baffle plate, and the number of broken gels was measured with respect to the stirring time. The results are shown in FIG.
第２図から明らかな如く、セルロースを混入した球状の
ゲル状物質は、セルロースを混入しなかったものに比べ
て、破壊され難いことが確認できた。As is clear from FIG. 2, it was confirmed that the spherical gel-like substance mixed with cellulose is less likely to be destroyed than the one not mixed with cellulose.
この結果からも、流動床型として使用される微生物又は
酵素の固定化担体として好適であることが確認された。From these results, it was confirmed that they are suitable as a carrier for immobilizing microorganisms or enzymes used as a fluidized bed type.
尚、セルロースに代えてベントナイトを用いた場合に
は、破壊個数は更に少なくなり望ましいことも確認でき
た。It was also confirmed that when bentonite was used instead of cellulose, the number of fractures was further reduced, which was desirable.
又、実施例１に於て、ポリアクリルアミドゲルを得る手
段として重合開始剤を混合して重合したが、これに限ら
ず、例えば電子線照射等によるラジカル重合を行なって
も良い。Further, in Example 1, a polymerization initiator was mixed and polymerized as a means for obtaining a polyacrylamide gel, but the present invention is not limited to this, and radical polymerization such as electron beam irradiation may be performed.
〔発明の効果〕 以上のように本発明によれば、これまで力学的強度が弱
く使用できなかったポリマー密度に於て十分な強度を持
たせることが可能になった。又、同じ物質で同程度の強
度を得るため、必要な合成試薬の量が減り、ゲルの製造
コストが減少する。 [Advantages of the Invention] As described above, according to the present invention, it has become possible to provide a polymer with sufficient mechanical strength, which has been so weak in mechanical strength that it could not be used. Further, since the same substance is obtained to the same degree of strength, the amount of synthetic reagents required is reduced, and the gel production cost is reduced.
第１図は本発明の実施例１と比較例１に於けるポリマー
密度と破壊強度との関係を示す図、第２図は同様に実施
例２と比較例２に於ける球状ゲルの撹拌時の破壊個数を
示す図である。FIG. 1 is a diagram showing the relationship between the polymer density and the breaking strength in Example 1 of the present invention and Comparative Example 1, and FIG. 2 is likewise when the spherical gel in Example 2 and Comparative Example 2 was stirred. It is a figure which shows the number of destruction of.
Claims (5)
するゲル状高分子物質内に、吸着性の強い物質又は微細
繊維状物質を混入することを特徴とするゲル状高分子物
質の補強方法。1. Reinforcement of a gel-like polymer substance, characterized in that a substance having a strong adsorptivity or a fine fibrous substance is mixed into a gel-like polymer substance constituting a carrier of an immobilized microorganism or an immobilized enzyme. Method.
するゲル状高分子物質は、アクリルアミド、アルギン酸
ナトリウム、ポリビニルアルコール、寒天ゲル、κ−カ
ラギーナン、又これらの高分子を主鎖に持つ高分子等で
あることを特徴とする特許請求の範囲第１項記載のゲル
状高分子物質の補強方法。2. A gel-like polymer substance that constitutes a carrier of an immobilized microorganism or an immobilized enzyme is acrylamide, sodium alginate, polyvinyl alcohol, agar gel, κ-carrageenan, or a polymer having these polymers in its main chain. The method for reinforcing a gel-like polymer substance according to claim 1, which is a molecule or the like.
炭、又はこれらの物質を主成分とするもの、或いはケイ
ソウ土、白土等の粉体であることを特徴とする特許請求
の範囲第１項記載のゲル状高分子物質の補強方法。3. A patent characterized in that the substance having strong adsorptivity is SiO 2 , Al 2 O 3 , activated carbon, or a substance containing these substances as a main component, or powder such as diatomaceous earth or clay. A method for reinforcing a gel-like polymer substance according to claim 1.
末であることを特徴とする特許請求の範囲第３項記載の
ゲル状高分子物質の補強方法。4. The method for reinforcing a gel-like polymer substance according to claim 3, wherein the substance having strong adsorptivity is a fine powder that is easily suspended in water.
或いは結晶性の高い合成高分子等であることを特徴とす
る特許請求の範囲第１項記載のゲル状高分子物質の補強
方法。5. The method for reinforcing a gel-like polymer substance according to claim 1, wherein the fine fibrous substance is a polymer such as cellulose or a synthetic polymer having high crystallinity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61181457A JPH0745593B2 (en) | 1986-08-01 | 1986-08-01 | Reinforcing method of gel polymer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61181457A JPH0745593B2 (en) | 1986-08-01 | 1986-08-01 | Reinforcing method of gel polymer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6337156A JPS6337156A (en) | 1988-02-17 |
| JPH0745593B2 true JPH0745593B2 (en) | 1995-05-17 |
Family
ID=16101093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61181457A Expired - Lifetime JPH0745593B2 (en) | 1986-08-01 | 1986-08-01 | Reinforcing method of gel polymer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0745593B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5336551A (en) * | 1992-12-14 | 1994-08-09 | Mizu Systems, Inc. | Reinforced polyvinyl alcohol hydrogels containing uniformly dispersed crystalline fibrils and method for preparing same |
| US6042839A (en) * | 1998-03-09 | 2000-03-28 | Color Access, Inc. | Powder compositions |
| CN115137710B (en) * | 2022-05-25 | 2024-04-12 | 广东药科大学 | Antibacterial volatile oil gel microsphere, preparation method thereof and microsphere mask comprising antibacterial volatile oil gel microsphere |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5222045A (en) * | 1975-08-12 | 1977-02-19 | Nitto Electric Ind Co Ltd | Highly elastic gels with improved thermal stability |
| JPS60255814A (en) * | 1984-05-31 | 1985-12-17 | Arakawa Chem Ind Co Ltd | Production of water-absorptive resin of improved gel strength |
-
1986
- 1986-08-01 JP JP61181457A patent/JPH0745593B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6337156A (en) | 1988-02-17 |
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