JPH075472B2 - External skin preparation - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to an external preparation for skin.

[Conventional technology]

Traditionally, Chiyorei-maitake is called the boar flesh due to its underground sclerotium, and it has been compounded in prescriptions such as Gorei-san, Ino-rei-to as an important medicine of ancient Chinese herbs. It is said to have an antipyretic effect. The fruiting bodies (mushrooms) on the above-ground part rarely occur in early summer and autumn and have been used for food and prized. Ergosterol and α-hydroxyterracanoic acid (α-
Hydroxyter-acosanoic acid) and polysaccharides are known, and in particular, the polysaccharides are said to have anti-cancer action.

The method for culturing the fungus body of Pleurotus cornucopia has already been disclosed in JP-A-52-28987.
JP-A No. 52-61283 and JP-A No. 52-61283 disclose that these methods include by-products and are not satisfactory for use as an external preparation for skin. A method for directly obtaining an extract from the sclerotia of Pleurotus cornucopiae is disclosed in JP-A-55-159796. Further, regarding the efficacy of the products obtained by these methods, those mainly related to the anti-cancer action, and none of them focus on the external preparation for skin.

[Problems to be solved by the invention]

As described above, the conventional technique has a problem in that the active ingredient of Chlorella edulis has not been sufficiently utilized as an external preparation for skin.

[Means for solving problems]

In order to solve the above-mentioned problems, the present invention relates to Polyporus umbellatus Fries.
(Polyporaceae)] fruit body or a mycelium extract separated and cultivated from fresh sclerotium or a dried product thereof is contained in a skin external preparation. The details will be described below.

First, the inoculum of Mycobacterium tuberculosis in the present invention is a mycelium confirmed to be free from miscellaneous bacteria by repeating subculture with an agar medium several times from fruit bodies or sclerotia immediately after harvesting.

Next, for culturing the inoculum of C. perfringens, it is advisable to use a plant medium of the family Gramineae (Poaceae). Here, the rice plants are rice, wheat, corn, etc., and the medium of these plants is the shredded plants as a main component and only normal water is added thereto, but if necessary, Water obtained by dissolving glucose, sucrose, malt extract, barley extract, yeast extract, peptone, or inorganic salts may be added to a mixture of rice bran, wheat bran, etc. And when culturing, inoculate the medium contained in a glass or plastic container with inoculum,
It is normally kept at 20 to 30 ° C for 7 to 15 days to allow it to reproduce sufficiently. When the culture is completed, the culture is used as it is or after it is pulverized to dry fine powder, for example, in a stainless steel tank or the like, using water or water containing 3% or less of acid or alkali,
Extract for 3 to 6 hours at 80 to 100 ° C. The extract is purified by centrifugation or filtration, and the obtained clear solution is concentrated under reduced pressure, and then acetic acid or trichloroacetic acid is added to
The protein is removed by adding 7% to dialysis. Furthermore, the dialysis solution is decompressed (for example, about 700 mm-H
g), concentrate the solution, add a water-soluble organic solvent (eg, ethyl alcohol, acetone, etc.) to the concentrated solution to 80% or more, collect the precipitate, and further collect this precipitate in 90% or more of the organic solvent. Wash with solvent or ether,
When dried, a dried product of the extract is obtained.

The form of the external preparation for skin in which the mycelium extract of Mytilus chinensis or the dried product thereof is mixed as the main component is not particularly limited, but an aqueous solution, an emulsion (skin milk, etc.), a cream, an ointment Those that are easy to apply are convenient. The amount of the mycelium extract or its dried product mixed in these external preparations for skin is preferably 0.05 to 10.0% (% is% by weight, the same applies hereinafter) of the total amount in terms of dried product. Because the amount mixed is 0.05%
If the amount is less than less than the expected effect, 10.0%
This is because when the amount exceeds a large amount, coloring and odor are generated, which is not preferable.

[Action]

The external preparation for skin according to the present invention exerts effects such as skin activation and tyrosinase activation by the action of the contained extract of Mytilus edulis.

〔Example〕

The mycelium free of miscellaneous bacteria obtained from several fruiting bodies and sclerotia of C. chinensis that grows naturally in the forests of Kita-Akita-gun, Akita Prefecture is used as an inoculum. The plant was inoculated into a rice plant medium and cultured at 27 ° C for about 10 days. Normal water is added to the obtained culture, extraction is performed at about 90 ° C for about 4 hours, the extract is filtered, the filtrate is concentrated under reduced pressure, and acetic acid is added to a concentration of about 4% for deproteinization. Natsuta. After that, it is dialyzed using a cellophane membrane, the dialyzed solution is concentrated under reduced pressure, ethyl alcohol is added to this to form a precipitate, and the precipitate is collected, washed with 95% ethyl alcohol, dried and extracted in powder form. I got a thing.

The properties of the obtained extract are shown in Table 1.

Table 1 Appearance Light gray to light brown non-hygroscopic powder Properties Soluble in water, insoluble in organic solvents such as alcohols, ethers, chloroform and benzene pH that does not show a certain melting point (1% aqueous solution) Neutral to slight Acid Mauritius reaction positive Anthuron sulfate reaction positive Tryptophan sulfate reaction positive Biuret reaction negative Xanthoprotein reaction negative Fehring's reaction negative glucose equivalent (phenol phenol method) 40% or more Tyrodinase activity inhibition rate (%) -42.20 Where, tyrodinase activity inhibition The rate was calculated by the following method. That is, the tyrosinase was obtained by purifying the product obtained from potato, 2 ml of 1 mg / ml solution, and 0.05% of L-tyrosine.
Solution 1 ml, phosphate buffer 1/15 ml (pH 6.8) solution 2 ml, Cu ²⁺
Ion (1% CuSO ₄ ) solution (0.05 ml) and 0.1% T. chinensis extract solution (2 ml) were added, and the absorbance at 640 μm was measured (the value is D), and then the mixture was kept at 37 ° C. for 1 hour, Measure the absorbance again (the value is D '), and at the same time, measure the absorbance of the solution containing 0.1% T. chinensis extract solution with normal water (the respective values are d and d'). , It is calculated from. As a control product, a 1% aqueous solution of Vitamin C was used in place of Chlorella edulis, and the same measurement was performed.
Since it was 6.24, it is conceivable that the 0.1% aqueous solution of Lentinus edodes extract promotes melanin production.

Examples 1 and 2: Cholesterus edulis extract and other components were blended in the blending ratios shown in Table 2, and completely dissolved at room temperature to obtain a lotion. In addition,% showing the mixing ratio is weight% (hereinafter the same). According to the sensory test of 20 adult women with respect to the obtained lotion, the effect of improving rough skin and oily skin was "clearly effective" (scoring this to 2 points), "slightly effective" (1 degree to this) 3) of “No effect” (scoring this to 0)
Evaluated in stages and calculated the total score of all members. The percentage of the total score (40 points), that is, (total score ÷ 40) x 100 is the efficacy (%), and the calculated efficacy (%) The value of is also shown in parentheses.

Comparative Example A lotion was prepared in the same manner as in Example 1 except that the extract of Agarnetus chinensis was not used. The evaluation results of the sensory test on the obtained lotion are also shown in Table 2, which is far inferior to both Examples 1 and 2.

Example 3: As the first phase,% octyldodecyl myristate 5.0 squalane 10.0 stearic acid 10.0 jojoba oil 3.0 natural vitamin E 0.1 polyoxyethylene sorbitan monooleate (20E.
O.) 1.0 Chlorella maitake extract 3.0 Xanthan gum 0.1 L-arginine 1.0 Concentrated glycerin 3.0 1,3-butylene glycol 5.0 Purified water 58.8 are prepared as the second phase. First, the first phase is dissolved at 80 ° C. The solution of the second phase, which was heated and dissolved at the same temperature, was added to the mixture, thoroughly mixed with a homogenizer, and then cooled to 35 ° C. to obtain a skin cream. When this skin cream was subjected to the same sensory test as in Example 1, the obtained evaluation results were the same as those in Examples 1 and 2.

Example 4: As the first phase,% white petrolatum 25 Stearyl alcohol 22 Propylene glycol 12 Sodium lauryl sulfate 1.5 Ethyl paraoxybenzoate 0.025 Propyl paraoxybenzoate 0.015 As the second phase, Chlorella edulis extract 5 Purified water All the rest are prepared respectively First, according to the 10th revised Japanese Pharmacopoeia hydrophilic ointment manufacturing method, the ingredients of the first phase are heated and dissolved to prepare a mixture at 75 ° C, and the solution of the second phase heated and dissolved at 75 ° C is added to this. The mixture was cooled with stirring to obtain a hydrophilic ointment. When this ointment was applied to people with rough skin and eczema, it was found to have excellent effects. In addition, the ointment of the present invention,
No problem occurred even if ordinary cosmetics, quasi-drugs, antiseptic germicides added to medicines, ultraviolet absorbers, vitamins, etc. were added.

〔effect〕

As described above, the external preparation for skin of the present invention has a skin activating effect in the extract of Mytilus edulis, which normalizes oily skin and rough skin, and also exhibits excellent effects on eczema and the like, and further produces melanin. Since it encourages, it can be expected that a moderately healthy color will appear for people with fair skin,
The significance of this invention can be said to be extremely great.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Yuji Shibayama 2-51, Doshomachi, Higashi-ku, Osaka, Osaka Iwase Coshua Co., Ltd.

Claims (1)

[Claims] 1. A Polygonaceae, Polygonaceae
porusumbellatus Fries (Polyporaceae)] a fruit body or a mycelium extract separated and cultured from fresh sclerotium or a dried product thereof, which is an external preparation for skin.