JPH0761476B2 - Highly concentrated alkaline wastewater treatment method - Google Patents
Highly concentrated alkaline wastewater treatment methodInfo
- Publication number
- JPH0761476B2 JPH0761476B2 JP7253493A JP7253493A JPH0761476B2 JP H0761476 B2 JPH0761476 B2 JP H0761476B2 JP 7253493 A JP7253493 A JP 7253493A JP 7253493 A JP7253493 A JP 7253493A JP H0761476 B2 JPH0761476 B2 JP H0761476B2
- Authority
- JP
- Japan
- Prior art keywords
- alkaline
- wastewater
- highly concentrated
- bacillus
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000004065 wastewater treatment Methods 0.000 title claims description 5
- 239000002351 wastewater Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 19
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000011282 treatment Methods 0.000 description 15
- 239000010802 sludge Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 5
- 241000193804 Planococcus <bacterium> Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102220201851 rs143406017 Human genes 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、食品産業などで排出さ
れる高濃度アルカリ廃水の処理をコンパクトに行うため
に、アルカリ性細菌を利用し、アルカリ廃水を中和或い
は希釈する操作を必要としないで高濃度或いは高アルカ
リのまま廃水処理をする方法に関する。BACKGROUND OF THE INVENTION The present invention utilizes alkaline bacteria to neutralize or dilute alkaline wastewater in order to compactly treat highly concentrated alkaline wastewater discharged in the food industry and the like. It relates to a method for treating wastewater with high concentration or high alkali.
【０００２】[0002]
【従来の技術】食品産業などの廃水の処理には一般的に
活性汚泥処理法が適していると言われており、従来より
この方法が用いられている。安定した活性汚泥処理を維
持するためには活性汚泥槽に供給する液は中性に、しか
も生物化学的酸素要求量（ＢＯＤ）は約２，０００mg／
ｌ以下に調整する事が好ましいと言われている。BACKGROUND ART It is said that an activated sludge treatment method is generally suitable for treating wastewater in the food industry, and this method has been used conventionally. In order to maintain stable activated sludge treatment, the liquid supplied to the activated sludge tank is neutral and the biochemical oxygen demand (BOD) is about 2,000 mg /
It is said that it is preferable to adjust it to 1 or less.
【０００３】従って、食品産業などで排出される高濃度
アルカリ廃水を従来法の活性汚泥処理法で行うために
は、予め中性近くまで中和する必要があり、しかも活性
汚泥処理に適した濃度にまで希釈する工程が欠かせな
い。Therefore, in order to carry out the high-concentration alkaline wastewater discharged in the food industry etc. by the conventional activated sludge treatment method, it is necessary to neutralize it to near neutrality in advance, and the concentration suitable for the activated sludge treatment. The process of diluting to 1 is essential.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、アルカリ性
細菌を使用する事により高濃度アルカリ廃水の中和工程
と希釈工程を省略し、廃水処理装置をコンパクトにする
ことが可能な高濃度アルカリ廃水処理方法を提供するも
のである。DISCLOSURE OF THE INVENTION The present invention provides a high-concentration alkaline wastewater capable of compacting the wastewater treatment apparatus by omitting the neutralization step and the dilution step of the high-concentration alkaline wastewater by using alkaline bacteria. It provides a processing method.
【０００５】[0005]
【問題を解決するための手段】上記の課題を解決するた
め、本発明は、３種類のアルカリ性細菌ＨＹ−５１２
５，バチルスsp. ＨＹ−２５６及びバチルスsp. ＨＹ−
３３３の一種類以上を用いる事を特徴とする高濃度アル
カリ廃水処理方法を提供する。高濃度アルカリ廃水の性状 下記の表１に１例として示す如く、高濃度アルカリ廃水
はpHが高く高アルカリ性で、しかもＢＯＤの高い高濃度
アルカリ廃水である。In order to solve the above problems, the present invention provides three types of alkaline bacteria HY-512.
5, Bacillus sp. HY-256 and Bacillus sp. HY-
A method for treating high-concentration alkaline wastewater, which is characterized by using one or more kinds of 333. Properties of High Concentration Alkaline Wastewater As shown in Table 1 below as an example, the high concentration alkaline wastewater is a high concentration alkaline wastewater having a high pH, a high alkalinity and a high BOD.
【０００６】[0006]
【表１】 [Table 1]
【０００７】アルカリ性細菌の性質 高濃度アルカリ廃水の処理に適した微生物を得るため、
保存菌、醤油工場及び自然界から分離した数千株の中か
ら、好気性条件下で高濃度アルカリ廃水に良く生育する
菌を選択し、３菌株が得られた。これらの菌株の形態学
的・生理学的・化学分類学的性質は表２〜表７に示す通
りである。 Properties of alkaline bacteria To obtain microorganisms suitable for treating highly concentrated alkaline wastewater,
From the preserved bacteria, the soy sauce factory, and thousands of strains isolated from the natural world, bacteria that grow well in highly concentrated alkaline wastewater under aerobic conditions were selected, and 3 strains were obtained. Morphological, physiological and chemotaxonomic properties of these strains are shown in Tables 2 to 7.
【０００８】[0008]
【表２】 [Table 2]
【０００９】[0009]
【表３】 [Table 3]
【００１０】[0010]
【表４】 [Table 4]
【００１１】[0011]
【表５】 [Table 5]
【００１２】[0012]
【表６】 [Table 6]
【００１３】[0013]
【表７】 [Table 7]
【００１４】アルカリ性細菌の同定 ＨＹ−２５６株及びＨＹ−３３３株は、バージマニュア
ルオブシステマティックバクテリオロジー２巻に従い分
類を行なったところ、バチルスsp. と同定された。即
ち、両菌株とも好気性、グラム染色陽性、桿菌、胞子を
形成する点においてバチルス属に属すると認められ、次
にカタラーゼ陽性、Ｖ−Ｐ反応陰性、ゼラチン液化陽
性、嫌気下での生育不能、７％食塩存在下での生育不
能、pH５．７での生育不能等の諸性質から、バチルス・
ファーマスの類縁菌であると考えられるが、糖からの酸
の産生が微弱であることより同定には至らず、バチルス
sp. とした。 Identification of Alkaline Bacteria The HY-256 and HY-333 strains were identified as Bacillus sp. By classification according to the Volume 2 of the Barge Manual of Systematic Bacteriology. That is, both strains were aerobic, Gram-staining positive, Bacillus, and found to belong to the genus Bacillus in terms of forming spores, followed by catalase-positive, VP-negative, gelatin liquefaction-positive, and inability to grow under anaerobic conditions. Due to various properties such as inability to grow in the presence of 7% sodium chloride and inability to grow at pH 5.7, Bacillus
It is considered to be a relative of Bacillus, but the acid production from sugar was weak, so it could not be identified.
sp.
【００１５】ＨＹ−５１２５株は、バージマニュアルオ
ブシステマティックバクテリオロジー２巻に従い分類を
行なったところ、好気性のグラム陽性球菌で菌体内ＤＮ
ＡのＧＣ含量が５０モル％であることよりプラノコッカ
ス（Planococcus ) 属に属する細菌と考えられたが、運
動性等の性状が異なり同定には至らなかった。ＨＹ−５
１２５株とプラノコッカス属等との性状の違いを表８に
示す。The HY-5125 strain was classified according to the Volume 2 of Barge Manual of Systematic Bacteriology and was found to be aerobic Gram-positive cocci and intracellular DN.
Since the GC content of A was 50 mol%, it was considered to be a bacterium belonging to the genus Planococcus ( Planococcus ), but its properties such as motility were different and it could not be identified. HY-5
Table 8 shows the difference in properties between the 125 strains and the genus Planococcus.
【００１６】[0016]
【表８】 [Table 8]
【００１７】尚、バチルスsp. ＨＹ−２５６は、通商産
業省工業技術院微生物工業技術研究所特許微生物寄託セ
ンターにＦＥＲＭ Ｐ−１３４１８として寄託され、バ
チルスsp. ＨＹ−３３３はＦＥＲＭ Ｐ−１３４１９と
して寄託され、ＨＹ−５１２５はＦＥＲＭ Ｐ−１３４
２０として寄託されている。本発明の高濃度アルカリ廃
水の処理は、処理すべき廃水中で前記のアルカリ性細菌
の少なくとも１株を好気的に培養することにより行う。
廃水処理の最初においては、比較的少量の適当な培地、
例えば高濃度アルカリ廃水に前記菌株を接種し、好気的
に培養して接種材料を調製する。次にこの接種材料を処
理すべき廃水に添加し、好気的に培養を行う。好気的培
養は、例えば通常の活性汚泥法の場合のように通気によ
り行うことができる。Bacillus sp. HY-256 has been deposited as FERM P-13418 at the Patent Microorganism Depositary Center of the Institute of Microbial Engineering, Ministry of International Trade and Industry, and Bacillus sp. HY-333 has been deposited as FERM P-13419. HY-5125 is FERM P-134
Deposited as 20. The treatment of the highly concentrated alkaline wastewater of the present invention is carried out by aerobically culturing at least one strain of the above-mentioned alkaline bacteria in the wastewater to be treated.
At the beginning of wastewater treatment, a relatively small amount of suitable medium,
For example, a highly concentrated alkaline wastewater is inoculated with the strain and aerobically cultured to prepare an inoculum. This inoculum is then added to the wastewater to be treated and aerobically cultivated. Aerobic culture can be carried out by aeration, as in the case of the usual activated sludge method.
【００１８】廃液処理は、回分式に行うことができる
が、処理が終了した廃水の大部分を除去したが残った処
理済廃水に新たな処理すべき廃水を加えることにより、
又は処理中又は処理後の廃水の１部を取り出して、処理
すべき新たな廃水に添加することにより、段階的又は半
連続的に行うことができる。あるいは１つの処理容器中
で、処理済液を連続的に取り出しながら、処理すべき新
たな廃液を連続的に加えて、連続法により行うこともで
きる。また、処理後に分離回収した菌体を再循環使用す
ることもできる。The waste liquid treatment can be carried out batchwise, but by removing most of the treated waste water but adding new waste water to the remaining treated waste water,
Alternatively, it is possible to carry out stepwise or semi-continuously by taking out a part of the wastewater during or after the treatment and adding it to a new wastewater to be treated. Alternatively, it is also possible to carry out a continuous process by continuously adding a new waste liquid to be treated while continuously taking out the treated liquid in one treatment container. In addition, the bacterial cells separated and collected after the treatment can also be recycled.
【００１９】[0019]
【実施例】次に、実施例により本発明をさらに具体的に
説明する。実施例１ ．アルカリ性細菌による高濃度アルカリ廃水の
処理 １）菌体量の測定方法 培養液２０mlを採取し、１８，０００ｇで１０分間遠心
分離する。この沈澱部分を純水で混合洗浄し遠心分離す
る操作を２回行った後、最後の沈澱部分を１０５℃２時
間乾燥し計量する。この量を菌体量（mg）とした。EXAMPLES Next, the present invention will be described more specifically by way of examples. Example 1 . Highly concentrated alkaline wastewater due to alkaline bacteria
Treatment 1) Method for measuring the amount of bacterial cells 20 ml of the culture solution is collected and centrifuged at 18,000 g for 10 minutes. The precipitated portion is mixed and washed with pure water and centrifuged twice, and the final precipitated portion is dried at 105 ° C. for 2 hours and weighed. This amount was defined as the bacterial cell amount (mg).
【００２０】２）ＴＯＣ測定方法 島津全有機体炭素計ＴＣ−５０００で測定し、元液のＴ
ＯＣ（全有機体炭素）から菌培養後の上澄液のＴＯＣを
差し引き元液のＴＯＣで割ってＴＯＣ除去率とした。 ３）種菌培養 分離したアルカリ性細菌は予め１８mm径試験管に高濃度
アルカリ廃水１０ml加え殺菌した培地で３０℃３日間前
培養した。2) TOC measuring method T of the original liquid was measured by Shimadzu total organic carbon meter TC-5000.
The TOC of the supernatant after bacterial culture was subtracted from OC (total organic carbon) and divided by the TOC of the original solution to obtain the TOC removal rate. 3) Inoculum culture The separated alkaline bacteria were pre-cultured at 30 ° C for 3 days in a medium sterilized by adding 10 ml of high-concentration alkaline waste water to an 18 mm diameter test tube in advance.
【００２１】４）高濃度アルカリ廃水の処理 ５００mlの坂口フラスコの高濃度アルカリ廃水１００ml
を加え、これを１２１℃１０分間オートクレーブ殺菌し
た。これに、どの坂口フラスコにも前培養液の添加量が
合計として５mlになるように添加した。３０℃３日間振
盪培養し高濃度アルカリ廃水を処理した。そして菌体
量、濁度及びＴＯＣ減少率を測定した。4) Treatment of highly concentrated alkaline waste water 100 ml of highly concentrated alkaline waste water in a 500 ml Sakaguchi flask
Was added and the mixture was sterilized by autoclaving at 121 ° C. for 10 minutes. This was added to any Sakaguchi flask so that the total amount of the preculture liquid added was 5 ml. The culture was carried out at 30 ° C. for 3 days with shaking to treat high-concentration alkaline wastewater. Then, the bacterial cell amount, turbidity, and TOC reduction rate were measured.
【００２２】菌株としてはＨＹ−５１２５、バチルスs
p. ＨＹ−２５６及びバチルスsp. ＨＹ−３３３の３菌
株の他 No.８， No.５４１４も用い、全５菌株の組み合
わせも考慮した処理を行った。その結果は表９の通りで
あり、３菌株用いるならばＨＹ−５１２５、バチルスs
p. ＨＹ−２５６及びバチルスsp. ＨＹ−３３３を用い
るのが有効である事が示された。As the strain, HY-5125, Bacillus s
Treatments were also carried out in consideration of combinations of all 5 strains using 3 strains of p. HY-256 and Bacillus sp. HY-333 as well as No. 8 and No. 5414. The results are shown in Table 9, and when three strains were used, HY-5125 and Bacillus
It has been shown to be effective to use p. HY-256 and Bacillus sp. HY-333.
【００２３】[0023]
【表９】 [Table 9]
【００２４】実施例２．活性汚泥菌とアルカリ細菌によ
る高濃度アルカリ廃水の連続処理 活性汚泥菌とアルカリ細菌によるプラント規模の廃水処
理装置を用いて、高濃度アルカリ廃水の連続処理を活性
汚泥菌とアルカリ細菌とについて行った結果、表１０の
処理結果を得た。ＢＯＤ容積負荷９kg−ＢＯＤ／ｍ3 ・
Ｄの場合、活性汚泥菌よりもアルカリ細菌の方が処理能
が優れていた。 Example 2 By activated sludge and alkaline bacteria
Continuous treatment of high-concentration alkaline wastewater using a plant-scale wastewater treatment device with activated sludge bacteria and alkaline bacteria As a result of continuous treatment of high-concentration alkaline wastewater with activated sludge bacteria and alkaline bacteria, the treatment results in Table 10 Got BOD volume load 9 kg-BOD / m 3 ·
In the case of D, the treating ability of the alkaline bacteria was superior to that of the activated sludge bacteria.
【００２５】[0025]
【表１０】 [Table 10]
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 庁内整理番号 ＦＩ 技術表示箇所 Ｃ１２Ｒ 1:07） ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 6 Identification code Internal reference number FI technical display area C12R 1:07)
Claims (1)
−５１２５、バチルス (Bacillus) sp. ＨＹ−２５６及
びバチルス sp.ＨＹ−３３３の１種類以上を培養するこ
とによって高濃度アルカリ廃水を処理する事を特徴とす
る廃水処理方法。1. HY of alkaline bacteria in alkaline wastewater
-5125, Bacillus (Bacillus) sp. HY-256 and wastewater treatment method characterized in that processing high concentration alkali waste by culturing one or more Bacillus sp.HY-333.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7253493A JPH0761476B2 (en) | 1993-03-30 | 1993-03-30 | Highly concentrated alkaline wastewater treatment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7253493A JPH0761476B2 (en) | 1993-03-30 | 1993-03-30 | Highly concentrated alkaline wastewater treatment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06277693A JPH06277693A (en) | 1994-10-04 |
| JPH0761476B2 true JPH0761476B2 (en) | 1995-07-05 |
Family
ID=13492116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7253493A Expired - Lifetime JPH0761476B2 (en) | 1993-03-30 | 1993-03-30 | Highly concentrated alkaline wastewater treatment method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0761476B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6846483B2 (en) | 1998-09-25 | 2005-01-25 | Council Of Scientific And Industrial Research | Microbial composition and a process useful for the neutralization of alkaline waste-waters |
| US20050191741A1 (en) | 1998-09-25 | 2005-09-01 | Council Of Scientific And Industrial Research | Microbial composition and a process useful for the neutralization of alkaline waste- waters |
| JP2002028693A (en) * | 2000-07-14 | 2002-01-29 | Kurabo Ind Ltd | Treatment of alkaline wastewater |
| JP3588613B2 (en) * | 2003-03-10 | 2004-11-17 | 株式会社神鋼環境ソリューション | Novel microorganism and method for treating organic solids using the microorganism |
| JP5448512B2 (en) * | 2009-03-17 | 2014-03-19 | プリマハム株式会社 | Microorganism having oil and fat decomposability and method for treating oil and fat-containing wastewater using the same |
-
1993
- 1993-03-30 JP JP7253493A patent/JPH0761476B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06277693A (en) | 1994-10-04 |
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