JPH0761875B2 - Method for producing active substance - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an active substance having an inhibitory action on various ionic reactions, and further, a physiological action such as an antiviral action, an anticancer action and an immune action.

The present inventor has found that an active substance obtained by adding ferrous sulfate to a large amount of aqueous hydrochloric acid solution is dissolved in water to convert the water into a nonionic reaction system, and suppresses various ionic reactions to suppress the reaction in normal water systems. It has been found that it induces a reaction that is significantly different from the case of using an ionic reaction system. And it is clear that all normal in-vivo reactions are carried out in the same nonionic reaction system as that formed by the active substance. Therefore, if the above substance is introduced into an abnormal living body, the nonionic reaction system in the living body is restored and the living body can be returned to normal. Thus, the above-mentioned active substance obtained according to the present invention has a physiological action such as a metal corrosion inhibition action based on an ionic reaction inhibition action, a salt damage removal action, a soil damage removal action as well as an antiseptic action, an antiviral action, an anticancer action and an immune action. Is to have.

The active substance obtained according to the present invention is obtained when a divalent iron salt is added to a large amount of aqueous hydrochloric acid solution. Specific examples of the method for producing the active substance of the present invention are shown below.

1.0mg of ferrous sulfate (FeSO ₄ · 7H _{2 O)} to stand overnight and dissolved with stirring was poured into 0.5N aqueous hydrochloric acid solution of 100 ml. The resulting insoluble substance is a biproduct, which is filtered off,
The filtrate is concentrated under reduced pressure and dried in a desiccator. 10 ml of 80% aqueous isopropyl alcohol solution is added to the obtained dry powder, and the eluted components are collected and concentrated under reduced pressure to remove the solvent and dry. By repeating the above extraction-concentration-drying operation several times, 0.6 mg of crystals are obtained.

In the present method, divalent iron salts such as ferrous chloride, ferrous nitrate, ferrous phosphate, ferrous formate, and ferrous acetate can be used in addition to ferrous sulfate.

A 5% by weight aqueous solution of the crystals was prepared, and 0.01 ml thereof was spotted at a position 3 cm inward from the lower end of the filter paper for paper chromatography No. 51A (2 cm × 40 cm), and n-butanol: acetic acid: water = 5: 1. : 4 volume ratio Mixture as developing solvent at 20 ℃, 15
Perform an upward expansion of time. After the development, the filter paper was dried and then sprayed with a 1 wt% potassium ferricyanide aqueous solution as a coloring reagent to the filter paper to confirm that the developed position of the crystal was Rf = 0.07 at one spot.

Then, a similar paper chromatographic test was conducted on a 1: 1 equivalent mixture of ferrous chloride and ferric chloride, and the result of development was 2 spots and Rf = 0.095 (FeC
l ₂ ) and Rf = 0.36 (FeCl ₃ ) were confirmed.

From the above paper chromatographic test, it is presumed that the crystal shows a property intermediate between ferrous chloride and ferric chloride and is not a mixture but a single compound.

The active substances of the present invention include, for example, sodium chloride, sodium sulfate, ammonium chloride, ammonium sulfate, diatomaceous earth,
It may be supported on an inorganic compound such as bentonite, silica or alumina, or an organic compound such as vitamins, hormones, proteins or lipids, and even in that case, the action of the active substance of the present invention does not change.

Examples of the present invention will be shown below.

Example 1 (Corrosion protection of metal) This example shows the corrosion resistance of the active substance according to the present invention. Corrosion of metals is caused by the generation of a corrosion current between the same kind of metal or different kinds of metal on the metal surface. Therefore, corrosion protection can be achieved by surface-treating the metal with a solution containing the active substance of the present invention.

A 0.2 cm × 5 cm × 5 cm iron piece was pre-cured, washed with diluted hydrochloric acid and distilled water, and dried, and then the active substance of the present invention (2.5 × 10 5
^-5 g / ml), hydrofluoric acid (1.2 x 10 ^-4 g / ml) and glucose (10 ^-3 g / ml) in a mixed solution of 200 ml,
It was treated at 30 ° C. for 30 minutes.

When the treated iron piece was subjected to a corrosion test in an HC1 stream,
The untreated iron pieces already showed significant corrosion after 1 hour, but the treated iron pieces did not show any corrosion in the 6-day corrosion test.

Example 2 (Removal of Salt Obstruction) This example shows the action of the active substance of the present invention to eliminate salt obstruction. Electrolyte solutions, especially seawater, are a major obstacle to ships and the maritime and coastal industries because of the metal ions they contain. Application of the active substances according to the invention makes it possible to eliminate these obstacles.

When the active substance of the present invention was added to natural seawater so that the ^{concentration} was 10 ⁻¹² g / ml, and iron powder, manganese powder, and copper powder were added and allowed to stand, untreated seawater contained all chlorides within 1 day. However, there was no change in the treated seawater for more than one year.

Example 3 (Modification of soil with continuous cropping failure) This example shows the effect of the active substance of the present invention to modify soil with continuous cropping failure. Depending on the crop, the continuous propagation of the same crop may cause violent growth of pathogenic bacteria in the soil, resulting in almost impossible harvesting. The root cause is the ionic reaction of inorganic and organic substances accumulated in soil. Therefore, these obstacles can be eliminated by introducing the active substance of the present invention.

The active substance of the present invention having a carrier of NaCl as a carrier was diluted with water to a ^{concentration} of 10 ⁻¹² g / ml in soil with strong continuous cropping accompanied by the breeding of Fusarium that occurred in the radish cultivated area (under Gifu prefecture). The diluted solution was applied to the extent that the soil was moistened, and daikon radish was planted in the usual manner.
As a result, all the crops in the treated soil grew healthy, and a yield index of 230 was obtained with respect to 100 in the control plot.

Example 4 (Keeping Freshness) This example shows the biological tissue preserving action of the active substance of the present invention.

Once the living tissue is separated from the living individual, the biological system is destroyed and the function of the tissue is lost, so that biological components such as proteins and carbohydrates immediately start to decompose. Since the active substance of the present invention is a basic substance that establishes a biological system,
Tissue disintegration does not occur in the solution containing the active substance of the present invention even after cutting out the living tissue from the living body.

Α-tocoph was added to 10 ml of a ^10-6 g / ml aqueous solution of the active substance of the present invention.
A mixture of 0.1 g each of erol and Ubiquinone (Co-enzymeQ ₇ ) was added and suspended, and then the above lipid portion was collected with ethanol. Thus, the above-mentioned lipid carrying the active substance of the present invention is obtained. 0.1% Tween-20 was added to this lipid as an interfacial agent.
g was added and dispersed in water, and diluted successively with distilled water to prepare a preparation solution having a lipid concentration of 2 × 10 ⁻¹² M / L.

Immediately after the white rat was slaughtered, the muscle tissue was placed in a bottle, the above-mentioned treatment solution was added, and the vessel was sealed and left at room temperature while leaving a part of the air layer. At the same time, as a control, muscle tissue was added with distilled water and tightly stoppered. As a result, in the control area, the tissue collapsed after 1 week, the microorganisms propagated, and the water became violently suspended. However, the tissue of the treated sample did not break down, no microbial growth occurred, and the liquid remained in the initial state for 1 month or longer while remaining transparent.

Example 5 (Survival of cuttings) This example shows the plant tissue regeneration effect of the active substance of the present invention.

The active substance of the present invention was treated in the same manner as in Example 4 with lipid (α-to
Copherol and Ubiquinone) were used as carriers and prepared with a solution having a lipid concentration of 10 ⁻⁷ M / L, and then cut branches of Japanese black pine were soaked in the prepared solution for 30 minutes, and then cut into a pot containing quartz sand. All the control plots died, but the treated black pine survived.

Example 6 (antiseptic and antifungal action) This example shows antiseptic and antifungal action of the active substance of the present invention. When the active substance of the present invention comes into contact with a microorganism, it acquires a new protein synthesis function according to the information possessed by the microorganism. This phenomenon is conventionally understood as an antigen-antibody reaction. This antibody can be used for antiseptic and antifungal of foods.

The active substance of the present invention was synthesized using magnesium chloride as a carrier to prepare a solution having a magnesium chloride concentration of 10 ⁻⁶ g / ml, which was used as a treatment liquid.

The pre-cured clams and rice cake pieces were allowed to stand at 32 ° C. for 3 days in an open system to propagate microorganisms. The generated microorganisms are treated with the above treatment liquid 10
Into a test tube containing ml, 0.5 g each of starch and peptone was placed, and the mixture was allowed to stand at 32 ° C. for 5 days. 100 ml of the resulting suspension 0.1 ml
It was added to ml of water, and this solution was irrigated with fresh clams and rice cake, sealed, and stored at room temperature. In each of the control plots, spoilage and mold formation were observed, but no microbial growth occurred in the treated samples for 3 weeks or longer.

Example 7 (antiviral action) This example shows the antiviral action of the active substance of the present invention.

When a substance or factor that modifies the biological system is externally invaded into the biological system maintained by the active substance of the present invention, the biological function is deteriorated and appears as a so-called lesion. The viral infection disorder is caused by the introduction of nucleic acid from the outside to destroy the biological system. Therefore, infection can be eliminated by effectively introducing the active substance of the present invention.

After ingesting TMV in tomato leaves using pre-cured tomato as a host plant and allowing it to grow in vivo, a juice was collected from the tomato leaves immediately before the test and diluted with water 500 times to prepare a test TMV suspension.

After applying carborundum to leaves of a tobacco plant cultivated after about 1 month, TMV suspension was diluted 2-fold with water as a control for one half of the test drug, and TMV suspension was applied to the other half of the same leaf. Each of the two-fold diluted with a test solution was applied to cotton by invading it.
The test solution used was 2.9 × 10 ⁻⁴ g / aqueous solution of the active substance of the present invention to which MgCl ₂ −6H ₂ O was added so as to be 1%. When the leaves were dry (approx. 30 minutes) after application, the remaining carborundum was washed with water and the plants were cultivated in a Koitron at 26 ° C.

The number of spots formed on each test leaf after 3 days of culture and the inhibition rate of the test substance were determined and the results are shown in Table 1.

[Brief description of drawings]

FIG. 1 shows the growth index in a culture solution containing the active substance of the present invention. In the figure, the-line shows normal cells ... the line shows abnormal cells

Continuation of the front page (51) Int.Cl. ⁶ Identification number Office reference number FI technical display location A23L 3/358 C09K 3/00 Z 17/02 H C23F 11/18 8414-4K

Claims (1)

[Claims] 1. A step of adding a salt of divalent iron to an aqueous hydrochloric acid solution to dissolve it, a step of removing the insoluble substance generated by allowing the solution to stand, and a step of boiling the solution from which the insoluble substance has been removed to obtain a dry matter. 3 Step 4 of extracting the obtained dried solid matter with a mixed solvent of isopropyl alcohol and water at room temperature and concentrating the extract to obtain crystals 4. Method for producing an active substance comprising the above steps 1, 2, 3, 4