JPS5835966B2 - Oral composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an oral composition, and more particularly to an oral composition that has the effect of suppressing the adhesion of dental plaque to teeth, and decomposing and removing dental plaque adhering to teeth.

In general, dental plaque is a dental deposit made up of various bacterial groups, bacterial metabolites, saliva components, etc., and the acids and proteolytic enzymes produced by oral bacteria in plaque are a cause of tooth decay and alveolar pyorrhea. has been done.

Therefore, suppressing the adhesion of dental plaque to teeth and removing dental plaque adhering to teeth are very effective means for preventing and treating dental caries and alveolar pyorrhea.

Conventionally, attempts have been made to decompose and dissolve dextran, which is a component of dental plaque, using dextran-degrading enzymes and dextranases for this purpose, but none have yet been found to be fully satisfactory.

While carrying out various studies on dextranase, the present inventors learned that a specific N-acyl-β-alanine salt has the effect of activating dextranase, and when it is allowed to coexist with dextranase. It was discovered that the adhesion of dental plaque to teeth can be suppressed and that dental plaque adhering to teeth can be efficiently removed, and the present invention was established.

That is, the present invention uses N cocoyl- as an enzyme activator.
One or more N-acyl- selected from the group consisting of N-methyl-β-alanine salt, N-lauroyl-N-methyl-β-alanine salt, and N-myristoyl-N-methyl-β-alanine salt. The purpose of the present invention is to provide a dextranase-containing oral composition containing β-alanine salt. It is extremely effective in preventing cavities and alveolar pyorrhea.

Next, the results of testing the dextranase activation activity of these N-acyl-β-alanine salts are shown.

Test 1 Dextranase 17V (100,000 units/su) was added at 0.
The enzyme solution was dissolved in 100 ml of 2M acetic acid buffer (1) H5,5), and various compounds shown in Table 1 below were added to each 1 ml portion of this enzyme solution, followed by heating at 40° C. for 10 minutes.

Next, 0.5% dextran (manufactured by Sigma,
After adding 1rrLl of a solution (molecular weight: 2 million) and reacting at 40°C for 10 minutes, the liberated reducing sugars were quantified by the Nomogyinelson method.

Reducing sugar was measured in the same manner when no compound was added, and the increase rate (%) in the amount of reducing sugar in the compound-added area relative to the non-additive area was defined as the activation rate.

Note that a heat-inactivated enzyme solution was used as a control.

The results are shown in Table 1 below. The N-acyl-β-alanine salt specifically activates dextranase, especially at relatively low concentrations, although it does not show or on the contrary reduces its activity.

Test 2 Arakashi female, 3 with Frayn heart infusion medium
Streptococcus mutans (S tr , mutans) cultured at 7°C for 18 hours was cultured in the following second step.
5% sucrose containing dissolved enzymes and/or sodium N-lauroyl N-methyl-β-alanine shown in the table.
- The seeds were placed in a test tube containing 5rr11 of infusion medium, and the test tube was held at an angle of 85° and cultured at 37° C. for 18 hours.

After culturing, the medium is discarded, the test tube is washed with physiological saline, and then 0.5N sodium hydroxide solution and 5 vinegar are added to uniformly suspend the bacterial cells attached to the tube wall, and the turbidity (550 m
μ transmittance) was measured.

Similarly, the turbidity was also measured for a control without the addition of the enzyme and the compound, and the ratio of the turbidity of the turbidity in the area to which the enzyme and/or the compound had been added was calculated when the turbidity of the control was set as 100.

The results are shown in Table 2. The bacterial cells attached to the tester wall are thought to be similar to dental plaque, and the lower the turbidity ratio, the stronger the plaque decomposition and removal effect.

As is clear from Table 2, dextranase and the N-
By using acyl-β-alanine salt in combination, dextranase is activated and decomposition of dental plaque is significantly promoted.

Thus, the oral composition of the present invention contains dextranase and the specific N-acyl-β-alanine salt as essential active ingredients.

The dextranase used may be any ordinary one, such as F
Lie, Penicillium genus, Aspergillus, ;< (Aspergillus)
Genus 1. Verticillium (Verticillium)
Microorganisms such as the genus M), the genus Spicaria, and the genus Streptomyces are cultured in a medium supplemented with a normal nutrient source or Textlan, and the supernatant is subjected to salting-out method, adsorption method,
Obtained by fractionation using solvent fractionation methods.

Although the properties vary slightly depending on the type of microorganism that produces the enzyme, the optimum pH is generally on the acidic side, and both degrade dextran into endo-type and exo-type, liberate reducing sugars, and reduce viscosity. It has the effect of reducing

As the N-acyl-β-alanine salt, salts such as alkali metal salts such as sodium and potassium salts, alkaline earth metal salts, and ammonium salts are used.

These may be used alone or in combination of two or more.

The blending ratio of dextranase and the N-acyl-β-alanine salt can be selected as appropriate depending on the desired effect;
From the viewpoint of efficacy and stability, 1.0 to 20% by weight of dextranase (as 5000 to 1 million units/l) based on the total amount of the composition, and o and o of N-acyl-β-alanine salts were added.
It is preferable to set it as ooi - 1.0 weight%.

Note that 1 unit of dextranase means the amount of enzyme that decomposes dextran and releases 1 μg/min of reducing sugar in terms of glucose.

The oral composition of the present invention can be prepared using conventional methods such as toothpaste, powdered toothpaste,
It can be in the form of dentifrices such as liquid toothpaste, oral cleansers such as mouthwash, denture cleansers, troches, and the like.

Other ingredients may be those that are normally added to these, for example, in the case of toothpaste, wetting agents such as glycerin and sorbitol, calcium diphosphate, calcium carbonate, silicic anhydride, aluminum hydroxide, calcium pyrophosphate, and insoluble metaphosphoric acid. Abrasives such as sodium, blowing agents such as sodium lauryl sulfate, sodium acylsarcosinate, and sucrose fatty acid esters, binders such as sodium carboxymethyl cellulose, carrageenan, sodium alginate, and bentonite, sweeteners, flavoring agents, and other monofluorophosphates. Contains sodium, bactericides, anti-inflammatory agents, etc.

Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A toothpaste was obtained by a conventional method according to the following recipe.

Actual row 2 According to the following prescription, I got Shu.

Mouthwatch by conventional method Growth minutes weight% saccharin sodium 0.05 ethanol 25.0 glycerin 5.0 Polyoxyethylene hardened castor oil 2.0 Incense fee 1.0 Dextranase (500,000 units) rank/2) 1, O N-lauroyl-N-methyl β-alanine sodium 0.0 water Adjust to ioo% Implementation row 3 Troche 2 was obtained by a conventional method according to the following recipe.

Growth minutes weight% gum arabic 6.0 glucose 72.0 Incense fee 1.0 Dexto place/g) Lanase (500,000 units) 0.1 N-lauroyl-N-methyl β-alanine sodium 1.0 water Adjust to 00%

Claims (1)

[Scope of Claims] 1. In a dextranase-containing oral composition, N-cocoyl-N-methyl-β-alanine salt, N-lauroyl-N-methyl-β-alanine salt, and N-myristoyl-N-methyl- An oral composition comprising one or more N-acyl-β-alanine salts selected from the group consisting of β-alanine salts. 2. Add 1.0 to 2 dextranase based on the total amount of the composition.
0% by weight (as 5000-100,000 units/g), the N
- The composition according to item 1 above, which contains 0.0001 to i,0% by weight of acyl-β-alanine salt. 3. The composition of item 1 or 2 above, which is in the form of a toothpaste. 4. The composition of item 1 or 2 above, which is in the form of a mouthwash. 5. The composition of item 1 or 2 above, which is in the form of a denture cleaning agent. 6. The composition of item 1 or 2 above, which is in the form of a troche.