JPH0755896B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to cosmetics containing a complex protein obtained from the parotid gland of cattle.

More specifically, the present invention provides a novel cosmetic composition capable of imparting an excellent moisturizing effect, protective effect, and skin beautiful effect to the skin by the action of the complex protein contained in the parotid gland of cattle. Is.

<Problems to be Solved by Conventional Techniques and Inventions> Conventionally, the amount of moisture in the skin has been deeply involved in maintaining the flexibility and beautiful skin of the skin, and also has a central role in the function of retaining the skin moisture. Is said to be the stratum corneum.

However, the water-retaining function of the skin, especially the stratum corneum, is that the barrier function of the stratum corneum decreases with the decrease in the secretion of the skin when the environment changes, for example, when the air is dried in the winter, and the skin's water retention ability If the transepidermal water loss (abbreviated as TWL hereafter) becomes greater than the above, or if water in the epidermis (especially the stratum corneum) is drawn out due to a physical cause (for example, washing), it will decrease significantly. It is known to end up.

On the other hand, even in a normal state, a state in which the water retention function of the skin is deteriorated due to changes in skin proteins with age, that is, hardening of collagen is called dry skin, and senile xerosis and the like are preferable examples. . Thus, the water content of the stratum corneum of the epidermis normally binds to the components of the stratum corneum to give the skin a moisturizing and beautiful skin, but the moisture content of the stratum corneum decreases due to environmental changes and aging, etc. The decrease in volume is
It is closely related to the dryness and brittleness of the skin, which causes rough skin. Therefore, various methods for improving these skin conditions and maintaining the normal water retention function of the skin have been studied.

Conventional methods have been to suppress TWL using occlusive agents and moisturizers, to enhance skin hydration effects, and to administer metabolic stimulants against skin aging to restore its function.・ There was a way to normalize.

Examples of the former include petrolatum ointment and a composition similar to the sebum found in JP-A-54-86630 to prevent the skin from drying by covering the skin surface, or ethylene glycol, polyhydric alcohol such as glycerin, PCA. -There is a method of increasing skin moisture by mixing mainly water-retaining components such as soda, saccharides, hyaluronic acid and various natural moisturizing factor (NMF) components. In any of these cases, in order to enhance the effect, The amount used must be increased, which causes an uncomfortable feeling such as stickiness, and conversely, if the amount used is suppressed, the effect is weak and the durability is not long-lasting. Furthermore, it could not be expected that these act directly on the cells of the skin to restore their function and develop the skin-beautifying effect.

On the other hand, as the latter example, steroid hormones typified by estrogen and adrenocortical hormone are generally used, but although they have an effect on the function recovery of skin cells, they are used in large amounts or for a long time. When it is used over a long period of time, there are problems such that local or systemic side effects are caused and the epidermis is thickened.

<Means for Solving the Problems> Therefore, the present inventor has solved these problems in the above-mentioned conventional methods, has a safe and excellent water retention ability, and activates skin cells to give a beautiful skin effect. As a result of intensive research to find a substance, the present inventors have found that the above-mentioned effect is present in a specific complex protein contained in the parotid gland of cattle, and completed the present invention.

That is, according to the present invention, 0.001 to 1 of the complex protein obtained as a precipitate was obtained by extracting water from the parotid gland of cattle with water under a weak alkaline condition of pH 7 to 9 and then adjusting the pH of the extract to 5.4.
The present invention relates to a cosmetic material characterized by being contained in a weight percentage.

The complex protein applied to the present invention is water-based extraction from the parotid gland of cattle, then concentrated if necessary, and further the pH of the extract.
It was obtained as a precipitate by adjusting to 5.4, which contains abundant various complex proteins with a molecular weight of several hundred thousand.

Many findings have already been obtained for such complex proteins as described above. For example, an extract extracted from water of the parotid gland of a cow is a complex protein having a molecular weight of about 130,000, and the same substance is obtained from the submandibular gland of a cow. Also,
JP-A-53-104714 discloses an aqueous extract of the parotid gland of a cow.
It has been reported that the extract obtained by adding acetone to the residual liquid from which the precipitate generated by adjusting the pH to 5.4 is removed and separating it is a complex protein having a molecular weight of about 66,000. on the other hand,
Extract from human saliva (molecular weight about 15,900) (Y.It
oJBiochem.1960,475) and complex protein E-3 (molecular weight approx.
55,000) (pharmaceutical journal 89.482,1969) 100-1 fraction (molecular weight about 350,000) (pharmaceutical journal 92 (7) 796-800,1972) and the like have been reported. Further, Japanese Patent Laid-Open No. 52-72887 discloses a novel peptide (active peptide H-1, molecular weight 740 and AA-1, obtained by reacting a salivary gland extract with a protease.
A molecular weight of 9,000) has also been reported.

The complex proteins obtained from these salivary glands or saliva have been previously administered intramuscularly or orally to reduce serum calcium, increase circulating white blood cell count, decrease serum citrate, and decrease serum acid phosphatase. It has been known to have physiological actions such as action, immune function enhancing action (eg, anti-cancer action), promoting histamine stress (central system) expression, and further promoting testosterone biosynthesis. Has hardly been studied, as it is in the cosmetic of the present invention,
Nothing was known about having a water-retaining function or a skin-beautifying effect on the skin.

Next, the method for producing the complex protein applied to the present invention will be described. Extraction of the complex protein from the parotid gland of bovine can be performed by a method known per se. In other words, the fresh glandular body collected from the parotid gland of a cow was chopped into
7-9 Preferably weakly alkaline water adjusted to pH 8 is added and extraction is carried out with stirring. Stirring is generally under cooling,
It will take several hours. When the extraction is complete, the mixture is filtered and the aqueous extract is separated. An inorganic acid such as hydrochloric acid may be added to the obtained aqueous extract to adjust the pH to 5.4, and the obtained precipitate may be separated and dried by a centrifugation method or the like, or
Furthermore, after adjusting the pH of the supernatant recovered by centrifugation to 5.4, acetone was added to this to cause precipitation and separation.
Use the dried one.

The thus obtained bovine parotid gland complex protein contains a high-molecular-weight complex protein that exerts a great effect on the skin as a main component, and also contains saccharides, lipids and the like.

Next, the content of the complex protein in the cosmetic of the present invention is
0.001% by weight or more of the total amount of cosmetics is selected. If the amount is less than the above range, the effects of water retention and skin beautifying effect are weak and the object of the present invention cannot be achieved. On the other hand, there is no particular restriction on the upper limit of the content, but even if the content is increased, a phenomenon of reaching the ceiling of the effect is seen and there is no effect of increasing the amount, and it is not economical, Depending on the type, there is a limit of dissolution (blending), etc., so that the content is usually 0.01 to 1% by weight.

Further, in the cosmetic of the present invention, in addition to the above complex protein, oils, surfactants, moisturizers containing polyhydric alcohols, oxidation stabilizers, pigments, ultraviolet absorbers, preservatives, and fragrances which are usually used in cosmetics. And the like can be blended in such a range that the protein in the complex protein does not deteriorate. The cosmetics of the present invention thus provided include, in addition to lotions, milks, creams, a wide range of undermakes, foundations and the like. The method for producing cosmetics is not particularly limited, and may be prepared, for example, by blending the above-mentioned complex protein as one of the aqueous phase components together with other components.

Here, in order to explain the present invention in more detail, an example of producing the composite protein according to the present invention will be shown.

Production Example 1 To 500 g of shredded parotid gland was added water 1 and a sodium hydroxide solution was added to adjust the pH to 8.0. This was stirred at 5 ° C. for 5 hours and then filtered to collect a filtrate. Water was added to the residue 1 again to adjust the pH to 8.0, and then 2 at 5 ° C.
The mixture was stirred for a period of time and then filtered to collect a filtrate, which was combined with the previous filtrate.

Next, 0.1N hydrochloric acid was added to this filtrate to adjust the pH to 5.4, and the mixture was left in a refrigerator overnight and then centrifuged at 4,000 rpm for 10 minutes to collect a precipitate. The supernatant was concentrated under reduced pressure to 1, the pH was adjusted to 5.4 again, and the mixture was left overnight in the refrigerator and then centrifuged to collect the precipitate. The obtained precipitates were combined and then dehydrated and dried to obtain 2.2 g of a complex protein.

Preparative Example 2 The supernatant obtained by removing the precipitate in Preparative Example 1 was reused with 0.1N.
Adjust the pH to 5.4 with hydrochloric acid, add acetone 1 to this,
I left it in the refrigerator overnight. The resulting precipitate was collected by centrifugation at 4,000 rpm for 10 minutes, dehydrated and dried to obtain 14 g of a complex protein.

In the present invention, by blending the complex protein obtained as described above, the water hydration effect of the complex protein is utilized to enhance the skin hydration effect to give a fresh skin, and to accompany aging. It is expected to have an effect of acting on the aging of skin cells and preventing the aging of the skin.

Therefore, the present inventor manufactured a cream containing the complex protein of the present invention shown in Example 1 described later, and conducted a practical use test to confirm its efficacy in preventing aging. As a comparative product, a cream prepared by removing the complex protein of the present invention from the cream shown in Example 1 (to increase the amount of water) was used. The test methods are shown below, and the results are shown in Table-1.

<Method> Twelve subjects with senile xerosis are extracted,
Randomly divided into A group (6 people) and B group (6 people),
The group A was made to use the cream of the present invention and the group B was made to use the cream of the comparative product three times a day (morning, noon and evening) for 3 months, and the skin condition after 3 months was confirmed.

Similarly, the present inventors confirmed the effect on the water retention effect by the following method using the lotion of Example 3 described later. The results are shown in Table-2.

<Method> 60 women with dry skin were extracted and
30 people were randomly divided into groups A and B, and the lotion of the present invention was added to group A, and the lotion of comparative product (without complex protein) was added to group B three times a day (morning, noon). , Night) and as needed for 1 month, and observed the feeling of use, the condition of the skin after use, and the morphology of the epidermis after 1 month.

As is clear from the results of Tables 1 and 2, the cosmetic containing the complex protein of the present invention improves the water retention of the skin, imparts a moist feeling, and has the effect of improving the aging of the skin. It is an excellent product that gives a beautiful skin effect. Further, no abnormality was found in the skin condition of the subject in any of the continuous tests, and it was confirmed that there was no problem in safety.

In addition, regarding the skin beautifying effect derived from this complex protein, it has not been clarified whether the cell activation effect based on the physiological activity of the complex protein is the main, or whether the water retention due to the high molecular weight protein is the main one. Is presumed to be due to these synergistic effects.

<Examples> Examples are shown below. The mixing ratio is% by weight.

Example 1, Cream (A) Cetanol 5.0 Beeswax 5.0 Microcrystalline Wax 5.0 Vaseline 5.0 Squalane 10.0 Glycerin Monostearate 2.0 PEO (20) Sorbitan Monostearate 2.0 (B) 1,3-Butylene Glycol 5.0 Glycerin 4.0 Ethylparaben 0.1 Complex Protein (Production Example 1) 0.1 Purified water 56.8 (Method) (A) and (B) are individually heated to 80 ° C. to dissolve them, and both are mixed and emulsified. Then cool to 30 ° C to make a cream.

Example 2, Emulsion (A) Cetanol 1.0 Vaseline 2.0 Beeswax 2.0 Squalane 10.0 PEO (20) Sorbitan monostearate 3.0 Sorbitan monostearate 1.5 (B) Carboxyl vinyl polymer (1% aq) 15.0 Potassium hydroxide 0.05 1,3 -Butylene glycol 8.0 Methylparaben 0.1 Royal jelly (raw) 0.1 Complex protein (Production Example 2) 0.05 Purified water 57.2 (Method) An emulsion was obtained in the same manner as in Example 1.

Example 3, lotion lotion Ethanol 7.0 1,3-butylene glycol 3.0 glycerin 2.0 POE (40) hydrogenated castor oil 0.5 citric acid 0.01 sodium citrate 0.1 methylparaben 0.05 complex protein (Production Example 1) 0.01 fragrance 0.1 purified water 87.23 (method ) The mixture was mixed at room temperature to obtain a lotion.

Example 4, Foundation (A) Stearic Acid 5.0 Glycerin Monostearate 2.5 Cetanol 1.0 Squalane 3.0 Liquid Paraffin 7.0 Isopropyl myristate 8.0 (B) 1,3-Butylene Glycol 3.0 Triethanolamine 1.2 Methylparaben 0.2 Complex Protein (Production Example 2) 0.001 Purified water 52.119 (C) Titanium oxide 8.0 Kaolin 5.0 Talc 2.0 Bentonite 1.0 Bengala 0.16 Yellow iron oxide 0.5 Black iron oxide 0.02 (D) Perfume 0.3 (Method) (C) is mixed and ground. (B) is mixed and dissolved, (C) is added and dispersed therein, and then heated to 75 ° C. (A) 80
The mixture was heated to ℃, added to (B) and emulsified. afterwards,
After cooling to 45 ° C and adding (D), the mixture was cooled to 30 ° C to obtain a creamy foundation.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-50-160413 (JP, A) JP-A-59-98727 (JP, A) JP-A-61-5006 (JP, A) JP-A-61- 210013 (JP, A)

Claims (1)

[Claims] 1. A composite protein obtained as a precipitate by water-extracting from the parotid gland of a bovine under mild alkaline condition of pH 7 to 9 and then adjusting the pH of the extract to 5.4 to 0.001 to 1% by weight.
Cosmetics characterized by containing.