JPH0774122B2 - How to store platelets - Google Patents

Description

TECHNICAL FIELD The present invention relates to an improvement in a method for preserving platelets, and in particular, it enables preservation for a long period of time while maintaining the aggregating ability of platelets.

[Prior Art] Platelets are made of flexible polyvinyl chloride as shown in FIG. 7, and whole blood is once collected using a parent bag 82 and a plurality of child bags 83. After collecting in the child bag 83 by a predetermined method, or by collecting directly in the child bag 83 by a predetermined method, or by collecting directly by a predetermined component blood sampling method, it may be similar to the child bag 83 shown in FIG. It is poured into a flexible vinyl chloride bag and stored with gentle shaking.

However, with these methods of preserving platelets, the maximum amount of platelets that can be preserved is 72 hours in Japan, and if the time is longer than this, the aggregating ability of platelets decreases and it is impossible to preserve them.

Thus, due to the short shelf life of storage, platelets had to be transfused to patients within 1 to 3 days after collection.

In particular, when supplying an HLA-compatible platelet transfusion to a patient, it was necessary to secure an HLA-compatible donor within 1 to 3 days, which was extremely difficult.

Therefore, as a result of intensive studies to solve the above problems, the present invention has found a method for preserving platelets that allows long-term preservation while maintaining the aggregating ability of platelets.

[Means for Solving the Problems] The present invention is a method for storing platelets using a platelet storage bag composed of an outer bag 1 containing a platelet preservation solution and a semipermeable membrane inner bag 2 containing a platelet. That is, after injecting platelets into the inner bag of the semipermeable membrane and injecting a predetermined amount of the platelet preservation solution into the outer bag 1, the platelet preservation bag is attached to the rotary agitator and shaken by rotation. The present invention provides a method for preserving platelets, in which the platelets are suspended while being applied and the nutrients are supplemented while being preserved.

[Operation] Platelets are enclosed in a semipermeable membrane inner bag having a predetermined volume. Therefore, platelets can be supplied with sufficient nutrition from the platelet preserving solution in the outer bag via the semipermeable membrane, and can be stored for a long period of time while maintaining a predetermined aggregation ability.

Furthermore, long-term storage can be surely performed by applying a fresh platelet preserving solution while appropriately shaking the platelet preserving bag by appropriate rotation.

[Embodiment] FIG. 1 shows a platelet storage bag used in the present invention, which is composed of an outer bag 1 and a semipermeable membrane inner bag 2 housed therein.

The outer bag 1 is formed by sealing the periphery of two soft synthetic resins in a bag shape, and a plurality of external liquid operation ports 3 are provided at the lower end portion.

The operation port 3 is sealed with a protector if necessary.

Further, a mounting hole 4 for a rotary agitator, which will be described later, is opened in a seal portion at a corner portion of the outer bag 1.

The inner bag 2 is made by sealing the openings of both ends of a tubular semipermeable membrane, and an inner liquid operation port 6 is provided at the upper end.

The inner liquid operation port 6 projects outside the outer bag 1.

The inner bag 2 is covered with a protective net 5.

The outer bag 1 of this embodiment is made of a comparative plastic such as a polyvinyl chloride / vinyl acetate copolymer or a polyolefin, and has a sheet thickness of 0.4 mm and a capacity of 2.000.

The inner bag 2 is made of, for example, regenerated cellulose, cellulose acetate, or other cellulose thread film or polyacrylonitrile, polymethylmethacrylate, polysulfone, polycarbonate, polyamide, polyethylene, polypropylene, or other synthetic film, and has a film thickness of 20 μm and a molecular weight cutoff. 1
The volume is 0,000 and 100 ml.

Next, a method for storing platelets using the platelet storage bag will be described.

First, platelets obtained by collecting a predetermined component of blood or the like are injected into the inner bag 2. This operation is performed in a clean bench.

First, as shown in FIG. 2, a connecting tube 11 is connected to a bag 10 in which 100 ml of concentrated platelet plasma (hereinafter abbreviated as “platelet”) is enclosed, and the other is connected to the internal liquid operation port 6.

This connecting tube 11 has infusion needles 12, 13 on both ends of the tube.
There is a clamp 14 in the middle of the tube.
Is attached.

The clamp 14 is first closed, the operation port 6 and the bag 10 are connected, and then the clamp 14 is opened to inject platelets by a drop.

Next, as shown in FIG.
The connection tube 11 having the same structure as the above is connected to the preservation solution bag 50 in which the MCDB-15 medium is encapsulated and injected by a drop.

Use a rotary agitator like the one shown in Fig. 4 at 22 ℃.
Install it in the incubator set to and set the above-mentioned platelet storage bag.

In this agitator, a rotary plate 60 of a box 61 is provided with an appropriate inclination, and the rotary plate 60 can be rotated by a drive mechanism built in the box 61. Further, stoppers 62 for fixing the storage bag are provided at four corners of the rotating plate 60, and the mounting holes 4 of the outer bag 1 can be fixed to the rotating plate 60 by the stoppers 62. .

After mounting the storage bag on the rotary plate 60, the rotary plate is gently rotated to start storage.

After the 120-hour storage is completed, the platelet storage bag is removed from the rotary agitator, and a recovery bag 70 as shown in FIG. 5 is prepared.

A guide tube 71 is connected to the recovery bag 70, and an infusion needle 72 is attached to the tip of the guide tube 71.

The guide tube 71 is loosely knotted at a portion near the bag as shown in the figure to form a loop 73 in advance.

Next, connect the infusion needle 72 of the collection bag 70 to the inner liquid operation port 6,
Collection bag 70 for collecting the internal liquid through the guide tube 71 due to the drop
To collect.

After the collection, the loop 73 of the guide tube 71 is tightly tied and sealed, and then the infusion needle 72 is removed from the inner fluid operation port 6.

Make another knot just next to the knot and seal it to remove unwanted parts.

Based on the examples carried out as described above, a platelet aggregation test was conducted to confirm the storage condition of platelets, and the results are shown in FIG. 6 and Table 1.

From these results, it is understood that the present invention has a higher platelet aggregating ability than the comparative example, and the platelets can be stored for a long time.

In the comparative example, 100 ml (5 units) obtained by blood sampling
The concentrated platelet plasma obtained in (1) was injected into a blood bag made of polyvinyl chloride with a volume of 300 ml, and gently shaken to preserve.

[Effects of the Invention] As described above, the present invention encloses platelets in a semipermeable membrane bag. To give shaking by rotation.
Due to such synergistic effects, platelets can be supplemented with sufficient nutrients from a platelet preservation solution through the semipermeable membrane, and can be stored for a long period of time while maintaining a predetermined aggregation ability.

Further, the present invention can be expected to further improve the platelet preservation effect by further researching the platelet preservation solution.

[Brief description of drawings]

FIG. 1 is a schematic view of a platelet storage bag used in the present invention, FIG. 2 is a schematic view of injecting platelets into an inner bag of the platelet storage bag, and FIG. 3 is a platelet storage in an outer bag of the platelet storage bag. FIG. 4 is a schematic view of injecting a liquid, FIG. 4 is a schematic view of a rotary agitator used in the present invention, FIG. 5 is a schematic view showing a state in which a cell recovery bag is connected to an inner bag, and FIG. FIG. 7 is a schematic diagram of a blood bag showing the results of the agglutination test. In the figure, 1 is an outer bag, 2 is an inner bag, 3 is an outer solution operation port, 5 is an inner bag protection net, 6 is an inner solution operation port, 10 is a bag containing platelets,
11 is a connecting tube, 50 is a preservation solution bag, and 70 is a platelet collection bag.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Mako Takeshi 1-26-7 Yokoyamadai, Sagamihara-shi, Kanagawa Kawasumi Chemical Co., Ltd. Saichihara Office Zenichi Wakimura

Claims (1)

[Claims] 1. A method of preserving platelets using a platelet storage bag comprising an outer bag containing a platelet preservation solution and an inner bag of a semipermeable membrane containing the platelets. After injecting into the inner bag and injecting a predetermined amount of the platelet preservation solution into the outer bag, the platelet preservation bag is attached to the rotary agitator, and the platelets are suspended while being shaken by rotation to replenish nutrition. Meanwhile, a method for preserving platelets, characterized by preserving.